論文 - 榊原 陽一
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Structural insights into tyrosine sulfation of CCR5 by human tyrosylprotein sulfotransferase-1 査読あり
Tanaka S., Asano H., Toyoda K., Nishiyori T., Kojo H., Kiyomatsu K., Kurogi K., Sakakibara Y., Nishimoto E., Teramoto T., Kakuta Y.
FEBS Journal 2026年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：FEBS Journal
Post-translational sulfation of the chemokine receptor CCR5 is involved in crucial biological processes such as viral infection and chemokine signaling. This sulfation can occur at the N-terminal tyrosine residues (Y3, Y10, Y14, and Y15) of CCR5 and is catalyzed by human tyrosylprotein sulfotransferases (hTPSTs) within the Golgi lumen. However, the detailed molecular mechanism by which these tyrosine residues are sulfated remains unresolved. To elucidate the mechanism, we determined the crystal structure of a soluble domain of hTPST1 bound to the sulfate donor product 3′-phosphoadenosine 5′-phosphate (PAP) and a modified 18-residue CCR5 peptide designed to isolate the Y3-centered binding mode, at 3.2 Å resolution, with six peptide residues ordered. This structure defines key interactions consistent with Y3 sulfation and is consistent with previous biochemical data. Based on the crystal structure and prior knowledge, we constructed peptide docking models for Y10, Y14, and Y15 sulfation, as well as full-length hTPST1-PAP-CCR5 docking models. The crystal structure provides experimental insight into Y3 recognition, whereas the docking models provide testable hypotheses for how the other sulfation sites may be accommodated in the context of CCR5.
DOI： 10.1111/febs.70597
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翻訳後修飾としてのチロシン硫酸化の機能解明 招待あり 査読あり
黒木 勝久, 奥田 菜月, 三城 恵美, 上地 珠代, 榊原 陽一
電気泳動 70 ( 1 ) 33 - 37 2026年
担当区分：責任著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本電気泳動学会
タンパク質の翻訳後修飾の一つであるチロシン硫酸化は，多細胞真核生物に広く存在し，分泌・膜タンパク質で重要な役割を担う．本研究ではまず，[<sup>35</sup>S]-硫酸標識法と電気泳動を組み合わせた古典的かつ確実な解析法を紹介した．さらに，ゼブラフィッシュを用いてチロシン硫酸化酵素TPSTの機能を解析した．ゼブラフィッシュには3種類の<i>tpst</i>遺伝子が存在し，ノックダウン実験によりTPST2が発生初期の体節形成に特に重要であることが示された．二次元電気泳動と質量分析により，50個のタンパク質スポットに変動が確認され，そのうち24種を同定した．中でもWnt-4aやSemaphorin-3aaが新規のチロシン硫酸化候補であり，特にWnt-4aの減少が体幹異常に関与する可能性が示唆された．本研究はTPSTと発生過程の関連を示唆するものである．
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細胞質硫酸転移酵素の新たな生理機能 査読あり
黒木 勝久, 榊原 陽一
生化学 97 ( 2 ) 214 - 219 2025年4月
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Iwamoto W., Ikeda T., Nishikawa H., Hirano M., Kinoshita H., Ono M., Kurogi K., Sakakibara Y., Suiko M., Yasuda S.
Bioscience, Biotechnology and Biochemistry 88 ( 9 ) 1081 - 1089 2024年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Indoxyl sulfate (IS), a uremic toxin, is a physiologically active sulfated metabolite, specifically in kidney failure patients. Our previous studies have shown that IS downregulates phagocytic immune function in a differentiated HL-60 human macrophage cell model. However, it remains unclear whether IS exerts similar effects on macrophage function in other cell types or in lipopolysaccharide (LPS)-sensitive immune cell models. Therefore, this study aimed to investigate the effects of IS on intracellular oxidation levels and phagocytic activity in a differentiated U937 human macrophage cell model, both in the absence and presence of LPS. Our results demonstrated that IS significantly increases intracellular oxidation levels and decreases phagocytic activity, particularly in cells activated by LPS. Furthermore, we found that 2-acetylphenothiazine, an NADH oxidase inhibitor, attenuates the effects of IS in LPS-activated macrophage cells. Representative antioxidants, trolox, α-tocopherol, and ascorbic acid, significantly mitigated the effects of IS on the macrophages responding to LPS.
DOI： 10.1093/bbb/zbae077
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A new type of sulfation reaction: C-sulfonation for α,β-unsaturated carbonyl groups by a novel sulfotransferase SULT7A1 査読あり
Katsuhisa Kurogi, Yoichi Sakakibara, Takuyu Hashiguchi, Yoshimitsu Kakuta, Miho Kanekiyo, Takamasa Teramoto, Tsuyoshi Fukushima, Takeshi Bamba, Jin Matsumoto, Eiichiro Fukusaki, Hiroaki Kataoka, Masahito Suiko
PNAS Nexas 3 ( 3 ) 097 2024年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Evolution and multiple functions of sulfonation and cytosolic sulfotransferases across species 査読あり
Katsuhisa Kurogi, Masahito Suiko, Yoichi Sakakibara
Bioscience, Biotechnology, and Biochemistry 88 ( 4 ) 368 - 380 2024年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effects of sturgeon fillet intake on top-ranked Japanese female long-distance runners 査読あり
Haraguchi, Naoki, Nakao, Hiroyuki, Sakakibara, Yoichi, Tamura, Hiroki, Nagahama, Kiyoko, Sakurai, Keiko, Sameshima, Hiroshi, Schauerte, Michael, Ikenoue, Tsuyomu, Katsuragi, Shinji
Journal of Obstetrics and Gynaecology Research 49 ( 8 ) 2164 - 2174 2023年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1111/jog.15711
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高速液体クロマトグラフィーを用いたアセトアミノフェン O- 硫酸体とチロシン O- 硫酸体の UV 検出による測定法 査読あり
森田千紘、元山優作、谷口玲央真、上田裕人、木下英樹、小野政輝、黒木勝久、榊原陽一、水光正仁、安田伸
東海大農紀要 42 1 - 8 2023年3月
記述言語：日本語 掲載種別：研究論文（大学，研究機関等紀要）
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チロシン硫酸化研究から食品機能評価など農学分野へのプロテオミクスの応用
榊原 陽一
日本プロテオーム学会誌 7 ( 2 ) 27 - 35 2022年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本プロテオーム学会
翻訳後修飾としてのチロシン硫酸化はタンパク質の分泌のためのシグナルや活性調節機構として考えられている．我々は長年このチロシン硫酸化に関与する酵素Tyrosylprotein Sulfotransferaseを研究し，その立体構造を解明し基質タンパク質認識機構などに新たな知見を得た．宮崎県地域結集型共同研究事業において，「食の機能を中心としたがん予防基盤技術創出」に関わり，新規食品機能評価技術として，プロテオミクスによるバイオマーカーの探索と定量，情報科学的なニューラルネットワーク解析による機能性推定を組み合わせた革新的な技術を確立した．さらに，タンパク質の修飾と食品機能の関係に着目し，食品の抗酸化作用をタンパク質の酸化傷害レベルを指標に評価するという考えに着想し，タンパク質のカルボニル化や<i>S</i>-ニトロシル化などのレドックスバランスに関連したタンパク質修飾の解析法を開発した．ブランド豚肉，地鶏などの地域の食材のプロテオーム解析や成分分析にも貢献した．
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プロテオミクス基盤技術を活用したブランド豚肉と個体識別の検討
黒木 勝久, 秋山 克樹, 榊原 陽一
電気泳動 66 ( 2 ) 97 - 102 2022年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本電気泳動学会
黒毛和牛やブランド豚肉を始めとした高付加価値食肉は系統種の交配と飼育条件の工夫により開発される．食肉偽装などの問題もあり，遺伝的・環境的要因を一度に解析できる手法を確立することで，効率的な優良育種とブランド肉の偽装鑑定への応用に期待できる．その一つとして，我々はプロテオミクス基盤技術を活用した解析を行っている．本稿では，豚肉に焦点を当てブランド肉鑑定および優良種豚選抜法への可能性を二次元電気泳動と質量分析計を用いて検討した結果を報告する．プロテオーム解析の結果，ブランド豚肉では解糖系に関するタンパク発現が大きく変動しており，環境要因が糖代謝に与える影響を見出すことが出来たと共に，ブランド豚肉を判別できるマーカータンパク質・ペプチドの候補を見出すことが出来た．さらに，優良育種に用いられるデュロック種と大ヨークシャー種の血清サンプルを用いた解析より，従来の系統的な選抜方法とは異なる新たな個体識別方法への可能性が示された．
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Kurogi K., Cao Y., Segawa K., Sakakibara Y., Suiko M., Uetrecht J., Liu M.C.
Biochemical Pharmacology 204 2022年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochemical Pharmacology
Nevirapine (NVP) is an effective drug for the treatment of HIV infections, but its use is limited by a high incidence of severe skin rash and liver injury. 12-Hydroxynevirapine (12-OH-NVP) is the major metabolite of nevirapine. There is strong evidence that the sulfate of 12-OH-NVP is responsible for the skin rash. While several cytosolic sulfotransferases (SULTs) have been shown to be capable of sulfating 12-OH-NVP, the exact mechanism of sulfation in vivo is unclear. The current study aimed to clarify human SULT(s) and human organs that are capable of sulfating 12-OH-NVP and investigate the metabolic sulfation of 12-OH-NVP using cultured HepG2 human hepatoma cells. Enzymatic assays revealed that of the thirteen human SULTs, SULT1A1 and SULT2A1 displayed strong 12-OH-NVP-sulfating activity. 1-Phenyl-1-hexanol (PHHX), which applied topically prevents the skin rash in rats, inhibited 12-OH-NVP sulfation by SULT1A1 and SULT2A1, implying the involvement of these two enzymes in the sulfation of 12-OH-NVP in vivo. Among five human organ cytosols analyzed, liver cytosol displayed the strongest 12-OH-NVP-sulfating activity, while a low but significant activity was detected with skin cytosol. Cultured HepG2 cells were shown to be capable of sulfating 12-OH-NVP. The effects of genetic polymorphisms of SULT1A1 and SULT2A1 genes on the sulfation of 12-OH-NVP by SULT1A1 and SULT2A1 allozymes were investigated. Two SULT1A1 allozymes, Arg37Asp and Met223Val, showed no detectable 12-OH-NVP-sulfating activity, while a SULT2A1 allozyme, Met57Thr, displayed significantly higher 12-OH-NVP-sulfating activity compared with the wild-type enzyme. Collectively, these results contribute to a better understanding of the involvement of sulfation in NVP-induced skin rash and provide clues to the possible role of SULT genetic polymorphisms in the risk of this adverse reaction.
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Investigation of radical scavenging effects of acetaminophen, p-aminophenol and their O-sulfated conjugates 国際共著
Morita Chihiro, Tokunaga Yuki, Ueda Yuto, Ono Masateru, Kinoshita Hideki, Kurogi Katsuhisa, Sakakibara Yoichi, Suiko Masahito, Liu Ming-Cheh, Yasuda Shin
The Journal of Toxicological Sciences 47 ( 10 ) 421 - 428 2022年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：一般社団法人 日本毒性学会
Acetaminophen (APAP) and <i>p</i>-aminophenol (<i>p</i>-AP) are the analogous simple phenolic compounds that undergo sulfate conjugation (sulfation) by cytosolic sulfotransferases. Sulfation is generally thought to lead to the inactivation and disposal of endogenous as well as xenobiotic compounds. This study aimed to investigate the antioxidative effects of <i>O</i>-sulfated form of APAP and <i>p</i>-AP, i.e., APAPS and <i>p</i>-APS, in comparison with their unsulfated counterparts. Using a 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay, the antioxidant capacity of APAPS was shown to be approximately 126-times lower than that of APAP. In contrast, <i>p</i>-APS displayed comparable activity as unsulfated <i>p</i>-AP. Similar trends concerning the suppressive effects of these chemicals on cellular O<sub>2</sub><sup>-</sup> radical generation were found using an activated granulocytic neutrophil cell model. Collectively, these results indicated that, depending on the presence of an additional “active site”, sulfation may not always decrease the antioxidant activities of phenolic compounds.
DOI： 10.2131/jts.47.421
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Yamamoto K., Yamada N., Endo S., Kurogi K., Sakakibara Y., Suiko M.
PLoS ONE 17 ( 8 August ) 2022年8月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：PLoS ONE
Polyphenols in plants are important for defense responses against microorganisms, insect herbivory, and control of feeding. Owing to their antioxidant, anti-cancer, and anti-inflammatory activities, their importance in human nutrition has been acknowledged. However, metabolism of polyphenols derived from mulberry leaves in silkworms (Bombyx mori) remains unclear. Sulfotransferases (SULT) are involved in the metabolism of xenobiotics and endogenous compounds. The purpose of this study is to investigate the metabolic mechanism of polyphenols mediated by B. mori SULT. Here, we identified a novel SULT in silkworms (herein, swSULT ST3). Recombinant swSULT ST3 overexpressed in Escherichia coli effectively sulfated polyphenols present in mulberry leaves. swSULT ST3 showed high specific activity toward genistein among the polyphenols. Genistein-7-sulfate was produced by the activity of swSULT ST3. Higher expression of swSULT ST3 mRNA was observed in the midgut and fat body than in the hemocytes, testis, ovary, and silk gland. Polyphenols inhibited the aldo-keto reductase detoxification of reactive aldehydes from mulberry leaves, and the most noticeable inhibition was observed with genistein. Our results suggest that swSULT ST3 plays a role in the detoxification of polyphenols, including genistein, and contributes to the effects of aldo-keto reductase in the midgut of silkworms. This study provides new insight into the functions of SULTs and the molecular mechanism responsible for host plant selection in lepidopteran insects.
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Acetaminophen 硫酸体の有機合成 査読あり
森田千紘、谷口玲央真、吉田実央、德永祐希、木下英樹＊、小野政輝、黒木勝久、榊原陽一、水光正仁、安田伸
東海大農紀要 41 39 - 46 2022年3月
記述言語：日本語 掲載種別：研究論文（大学，研究機関等紀要）
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Molecular cloning and characterization of common marmoset SULT1C subfamily members that catalyze the sulfation of thyroid hormones. 査読あり
Kurogi, K., Manabe, Y., Liu, M.-C., Suiko, M., Sakakibara, Y.
Biosci. Biotechnol. Biochem. 85 2113 - 2120 2021年8月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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The crystal structure of mouse SULT2A8 reveals the mechanism of 7α-hydroxyl, bile acid sulfation
Teramoto T., Nishio T., Kurogi K., Sakakibara Y., Kakuta Y.
Biochemical and Biophysical Research Communications 562 15 - 20 2021年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochemical and Biophysical Research Communications
Bile acids play essential roles in facilitating the intestinal absorption of lipophilic nutrients as well as regulation of glucose, lipid, and energy homeostasis via activation of some receptors. Bile acids are cytotoxic, and consequently their concentrations are tightly controlled. A critical pathway for bile acid elimination and detoxification is sulfation. The pattern of bile acid sulfation differs by species. Sulfation preferentially occurs at the 3α-OH of bile acids in humans, but at the 7α-OH in mice. A recent study identified mouse cytosolic sulfotransferase 2A8 (mSULT2A8) as the major hepatic 7α-hydroxyl bile acid-sulfating enzyme. To elucidate the 7α-OH specific sulfation mechanism of mSULT2A8, instead of 3α-OH specific sulfation in humans, we determined a crystal structure of mSULT2A8 in complex with cholic acid, a major bile acid, and 3′-phosphoadenosine-5′-phosphate, the sulfate donor product. Our study shows that bile acid-binding mode of mSULT2A8 and how the enzyme holds the 7α-OH group of bile acids at the catalytic center, revealing that the mechanism underlying 7α-OH specific sulfation. The structure shows the substrate binds to mSULT2A8 in an orientation perpendicular to that of human 3α-hydroxyl bile acid-sulfotransferase (hSULT2A1). The structure of the complex provides new insight into species different bile acid metabolism.
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Proteolytic maturation of the Outer Membrane c-type cytochrome OmcZ by Subtilisin-like Serine Protease is Essential for Optimal Current Production by Geobacter sulfurreducens 査読あり
Kai A, Tokuishi T, Fujikawa T, Kawano Y, Ueki T, Nagamine M, Sakakibara Y, Suiko M, Inoue K.
Appl Environ Microbiol. AEM.02617-20 2021年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1128/AEM.02617-20
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Varietal differences in flavonoid and antioxidant activity in Japanese soybean accessions 査読あり
Arifin, H.A, Hashiguchi, T, Nagahama, K, Hashiguchi, M., Muguerza, M., Sakakibara, Y., Tanaka, H., Akashi, R.
Bioscience, biotechnology, and biochemistry 85 ( 4 ) 916 - 922 2021年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effects of indole and indoxyl on the intracellular oxidation level and phagocytic activity of differentiated hl-60 human macrophage cells 査読あり
Tsutsumi, S, Tokunaga, Y, Shimizu, S, Kinoshita, H., Ono, M., Kurogi, K, Sakakibara, Y, Suiko, M, Liu, M.-C, Yasuda, S
Journal of Toxicological Sciences 45 ( 9 ) 569 - 579 2020年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Investigation of the effects of indoxyl sulfate, a uremic toxin, on the intracellular oxidation level and phagocytic activity using an HL-60-differentiated human macrophage cell model. 査読あり
Tsutsumi, S., Tokunaga, Y., Shimizu, S., Kinoshita, H., Ono, M., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C., Yasuda, S.
Biosci. Biotechnol. Biochem. 84 1023 - 1029 2020年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effect of SULT2B1 genetic polymorphisms on the sulfation of dehydroepiandrosterone and pregnenolone by SULT2B1b allozymes 査読あり
Alherz, F.A., El Daibani, A.A., Sakakibara, Y. Suiko, M., Kurogi, K., Liu, M.-C.
Molecular and Cellular Endocrinology 496 110535 2019年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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レトルト加工及び保蔵期間が鶏肉のイミダゾールジペプチド含有量に及ぼす影響 査読あり
神力(長友)はるな、近藤知巳、永濵清子、福井敬一、黒木勝久、水光正仁、榊原陽一.
日本食品科学工学会誌 66 210 - 214 2019年6月
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Identification of zebrafish steroid sulfatase and comparative analysis of the enzymatic properties with human steroid sulfatase. 査読あり
Kurogi K, Yoshihama M, Williams FE, Kenmochi N, Sakakibara Y, Suiko M, Liu MC.
Journal of Steroid Biochemistry and Molecular Biology 185 110 - 117 2019年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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完熟きんかん摂食に伴う血清βクリプトキサンチン濃度の検討-ランダム化非盲検非摂食同時対照群間比較試験- 査読あり
有村 保次, 武安 智樹, 米澤 ゆう子, 永濵 清子, 神力 はるな, 近藤 知巳, 上野 浩晶, 松元 信弘, 江藤 望, 榊原 陽一, 水光 正仁, 片岡 寛章
薬理と治療 47 ( 1 ) 65 - 75 2019年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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The critical role of His48 in mouse cytosolic sulfotransferase SULT2A8 for the 7-hydroxyl sulfation of bile acids. 査読あり
Shimohira, T., Kurogi, K., Liu, M.-C., Suiko, M., Sakakibara, Y.
Biosci. Biotechnol. Biochem. 82 1359 - 1365 2018年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sulfation of catecholamines and serotonin by SULT1A3 allozymes. 査読あり
Bairam, A.F., Rasool, M.I., Alherz, F.A., Abunnaja, M.S., El Daibani, A.A., Gohal, S.A., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
Biochem. Pharmacol. 151 104 - 113 2018年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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On the Role of Genetic Polymorphisms in the Sulfation of Cholesterol by Human Cytosolic Sulfotransferase SULT2B1b. 査読あり
Alherz, F.A., El Daibani, A.A., Bairam, A.F., Abunnaja. M.S., Rasool, M.I., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
J. Biochem. 164 215 - 221 2018年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1093/jb/mvy042
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Effects of Genetic Polymorphisms on the Sulfation of Dehydroepiandrosterone and Pregnenolone by Human Cytosolic Sulfotransferase SULT2A1. 査読あり
Abunnaja, M.S.、Alherz, F.A.、El Daibani, A.A.、Bairam, A.F.、Rasool, M.I.、Gohal, S.A.、Kurogi, K.、Suiko, M.、Sakakibara, Y.、Liu, Mi.-C.
Biochem. Cell Biol. 96 655 - 662 2018年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Radical scavenging effects of 1-naphthol, 2-naphthol, and their sulfate-conjugates 査読あり
Sugahara Shintaro, Fukuhara Kumiko, Tokunaga Yuki, Tsutsumi Shuhei, Ueda Yuto, Ono Masateru, Kurogi Katsuhisa, Sakakibara Yoichi, Suiko Masahito, Liu Ming-Cheh, Yasuda Shin
Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｔｏｘｉｃｏｌｏｇｉｃａｌ Ｓｃｉｅｎｃｅｓ 43 ( 3 ) 213 - 221 2018年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：一般社団法人 日本毒性学会
1-Naphthol (1-Nap) and 2-naphthol (2-Nap) are phenolic isomers that may be subjected to sulfate conjugation <i>in vivo</i>. Phase-II sulfate conjugation of phenolic compounds is generally thought to result in their inactivation. This study aimed to investigate the antioxidative effects of 1-NapS and 2-NapS, in comparison with their unsulfated counterparts, using established free radical scavenging assays. Based on the calculated EC<sub>50</sub> values, 1-NapS resulted in 5.60 to 7.35-times lower antioxidative activity than 1-Nap. In contrast, 2-NapS showed comparable activities as did the unsulfated 2-Nap. Collectively, the results obtained indicated that sulfate conjugation of the Nap isomers did not always result in the decrease of their antioxidant activity, and the antioxidant activity that remained appeared to depend on the position of sulfation.
DOI： 10.2131/jts.43.213
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Effects of Human SULT2A1 Genetic Polymorphisms on the Sulfation of Tibolone 査読あり
Miller, E., Zalzala, M.H., Abunnaja, M.S., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
Eur. J. Drug Metab. Pharmacokinetics 2018年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Structural basis for the broad substrate specificity of the human tyrosylprotein sulfotransferase-1 査読あり
Tanaka S., Nishiyori T., Kojo H., Otsubo R., Tsuruta M., Kurogi K., Liu M., Suiko M., Sakakibara Y., Kakuta Y.
Scientific Reports 7 ( 1 ) 2017年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Scientific Reports
© 2017 The Author(s). Tyrosylprotein sulfotransferases (TPSTs) are enzymes that catalyze post-translational tyrosine sulfation of proteins. In humans, there are only two TPST isoforms, designated TPST1 and TPST2. In a previous study, we reported the crystal structure of TPST2, which revealed the catalytic mechanism of the tyrosine sulfation reaction. However, detailed molecular mechanisms underlying how TPSTs catalyse a variety of substrate proteins with different efficiencies and how TPSTs catalyze the sulfation of multiple tyrosine residues in a substrate protein remain unresolved. Here, we report two crystal structures of the human TPST1 complexed with two substrate peptides that are catalysed by human TPST1 with significantly different efficiencies. The distinct binding modes found in the two complexes provide insight into the sulfation mechanism for these substrates. The present study provides valuable information describing the molecular mechanism of post-translational protein modifications catalysed by TPSTs.
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完熟きんかん摂食に伴う抗疲労効果の検討—ランダム化非盲検非摂食同時対照比較群間試験— 査読あり
武安智樹、有村保次、永濵清子、神力はるな、榊原陽一、水光正仁、片岡寛章
薬理と治療 45 1967 - 1975 2017年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Kurogi K., Yoshihama M., Horton A., Schiefer I., Krasowski M., Hagey L., Williams F., Sakakibara Y., Kenmochi N., Suiko M., Liu M.
Journal of Steroid Biochemistry and Molecular Biology 174 120 - 127 2017年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Steroid Biochemistry and Molecular Biology
© 2017 Elsevier Ltd 5α-Cyprinol 27-sulfate is the major biliary bile salt present in cypriniform fish including the zebrafish (Danio rerio). The current study was designed to identify the zebrafish cytosolic sulfotransferase (Sult) enzyme(s) capable of sulfating 5α-cyprinol and to characterize the zebrafish 5α-cyprinol-sulfating Sults in comparison with human SULT2A1. Enzymatic assays using zebrafish homogenates showed 5α-cyprinol-sulfating activity. A systematic analysis, using a panel of recombinant zebrafish Sults, revealed two Sult2 subfamily members, Sult2st2 and Sult2st3, as major 5α-cyprinol-sulfating Sults. Both enzymes showed higher activities using 5α-cyprinol as the substrate, compared to their activity with DHEA, a representative substrate for mammalian SULT2 family members, particularly SULT2A1. pH-Dependence and kinetics experiments indicated that the catalytic properties of zebrafish Sult2 family members in mediating the sulfation of 5α-cyprinol were different from those of either zebrafish Sult3st4 or human SULT2A1. Collectively, these results imply that both Sult2st2 and Sult2st3 have evolved to sulfate specifically C 27 -bile alcohol, 5α-cyprinol, in Cypriniform fish, whereas the enzymatic characteristics of zebrafish Sult3 members, particularly Sult3st4, correlated with those of human SULT2A1.
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Δ<sup>4</sup>-3-ketosteroids as a new class of substrates for the cytosolic sulfotransferases 査読あり
Hashiguchi T., Kurogi K., Shimohira T., Teramoto T., Liu M., Suiko M., Sakakibara Y.
Biochimica et Biophysica Acta - General Subjects 1861 ( 11 ) 2883 - 2890 2017年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochimica et Biophysica Acta - General Subjects
© 2017 Elsevier B.V. Cytosolic sulfotransferase (SULT)-mediated sulfation is generally known to involve the transfer of a sulfonate group from the active sulfate, 3′-phosphoadenosine 5′-phosphosulfate (PAPS), to a hydroxyl group or an amino group of a substrate compound. We report here that human SULT2A1, in addition to being able to sulfate dehydroepiandrosterone (DHEA) and other hydroxysteroids, could also catalyze the sulfation of Δ 4 -3-ketosteroids, which carry no hydroxyl groups in their chemical structure. Among a panel of Δ 4 -3-ketosteroids tested as substrates, 4-androstene-3,17-dione and progesterone were found to be sulfated by SULT2A1. Mass spectrometry analysis and structural modeling supported a reaction mechanism which involves the isomerization of Δ 4 -3-ketosteroids from the keto form to an enol form, prior to being subjected to sulfation. Results derived from this study suggested a potential role of SULT2A1 as a Δ 4 -3-ketosteroid sulfotransferase in steroid metabolism.
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疲労感を有する健常人に対する完熟きんかんの有効性を探索する非対照・非盲検試験 査読あり
永濵清子、有村保次、神力はるな、米澤ゆう子、松尾彩子、上野浩晶、松元信弘、江藤望、榊原陽一、水光正仁、片岡寛章
薬理と治療 45 1831 - 1842 2017年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Identification and characterization of the zebrafish glutathione S-transferase Pi-1
Abunnaja M., Kurogi K., Mohammed Y., Sakakibara Y., Suiko M., Hassoun E., Liu M.
Journal of Biochemical and Molecular Toxicology 31 ( 10 ) 2017年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Biochemical and Molecular Toxicology
© 2017 Wiley Periodicals, Inc. Zebrafish has in recent years emerged as a popular vertebrate model for use in pharmacological and toxicological studies. While there have been sporadic studies on the zebrafish glutathione S-transferases (GSTs), the zebrafish GST gene superfamily still awaits to be fully elucidated. We report here the identification of 15 zebrafish cytosolic GST genes in NCBI GenBank database and the expression, purification, and enzymatic characterization of the zebrafish cytosolic GST Pi-1 (GSTP1). The cDNA encoding the zebrafish GSTP1 was cloned from a 3-month-old female zebrafish, expressed in Eschelichia coli host cells, and purified. Purified GSTP1 displayed glutathione-conjugating activity toward 1-chloro-2,4-dinitrobenzene as a representative substrate. The enzymatic characteristics of the zebrafish GSTP1, including pH-dependency, effects of metal cations, and kinetic parameters, were studied. Moreover, the expression of zebrafish GSTP1 at different developmental stages during embryogenesis, throughout larval development, onto maturity was examined.
DOI： 10.1002/jbt.21948
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食品成分の抗酸化能の複合的評価について 査読あり
近藤 知巳, 上橋 朋佳, 渡辺 朋子, 河野 朝美, 黒木 勝久, 福井 敬一, 水光 正仁, 榊原 陽一
日本食品科学工学会誌 64 ( 9 ) 457 - 463 2017年9月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：社団法人 日本食品科学工学会
近年，食品成分による抗酸化に関する研究は盛んに行われており，抗酸化能を評価する手法についても多くの手法が開発されている．化学反応を用いた評価手法は，簡便な操作で検討できることや特殊な機器を必要としないことなどのメリットがある一方で必ずしも，実際の生体内における抗酸化活性と同等の評価になりえないなどのデメリットがあると言われている．そこで本研究では，比較的簡便に抗酸化能を評価できる化学的手法とより生体条件に近いと考えられる細胞応答を基盤としたルシフェラーゼレポーターアッセイ系による抗酸化ストレス作用を抗酸化能の評価系として用い，食品成分の抗複合的な抗酸化活性の評価の可能性について検討を行った．検討の結果，抗酸化活性の高いポリフェノール類であっても，評価法の選択により抗酸化活性に差が生じることや化学的な評価法とレポーターアッセイのような細胞応答を基盤とした評価法を組み合わせることで，複合的な抗酸化活性の知見を得ることが出来る可能性が示された．今後，食品の抗酸化活性の評価は単に抗酸化活性を有する化合物の有無だけでなく，生体内での抗酸化酵素誘導活性を含む評価をすることで，多角的な抗酸化活性を評価出来る可能性があるのではないかと考えられる．
DOI： 10.3136/nskkk.64.457
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Sulfation of vitamin D3-related compounds: Identification and characterization ofthe responsible human cytosolic sulfotransferases. 査読あり
Kurogi, K., Sakakibara, Y., Suiko M, Liu MC.
FEBS Lett. 591 2417 - 2425 2017年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Shimohira, T., Kurogi, K., Hashiguchi, T., Liu, M-C., Suiko, M., Sakakibara, Y.
Journal of Bioscience and Bioengineering 124 ( 1 ) 84 - 90 2017年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Bioscience and Bioengineering
© 2017 The Society for Biotechnology, Japan Dietary polyphenols present in fruits and vegetables have been reported to manifest beneficial health effects on humans. Polyphenol metabolites including their sulfated derivatives have been shown to be biologically active. Primarily due to the difficulty in preparing regiospecific sulfated polyphenols for detailed investigations, the exact functions of sulfated polyphenols, however, remain unclear. The current study aimed to develop a procedure for the regioselective production of sulfated polyphenols using Escherichia coli cells expressing human cytosolic sulfotransferases (SULTs). Two regioisomers of sulfated genistein were produced by E. coli cells expressing human SULT1A3, SULT1C4, or SULT1E1, and purified using Diaion HP20 resin, followed by high pressure liquid chromatography (HPLC). Structural analysis using mass spectrometry (MS) and nuclear magnetic resonance (NMR) revealed that E. coli cells expressing SULT1A3 preferentially produced genistein 4′-sulfate, whereas E. coli cells expressing SULT1C4 preferentially produced genistein 7-sulfate. To improve the bioproductivity, the effects of several factors including the concentrations of glucose and SO 4 2− , and growth temperature were investigated. The bioproduction procedure established in this study will be valuable for the production of regioselective sulfated polyphenols for use in future studies on their biological functions.
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Characterization of a novel allergenic protein from the octocoral Scleronephthya gracillima (Kuekenthal) that corresponds to a new GFP-like family named Akane. 査読あり
Kato, Y., Jimbo, M., Sakakibara, Y., Onizuka, R., Takahashi, T., Matsuhashi, S., Mita, H., Amada, K., Imahara, Y., Tanabe, K., Toda, A., Kamiya, H.
Luminescence 2017年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Takao H., Takao H., Hirabayashi K., Nishigaya Y., Kouriki H., Nakaniwa T., Hagiwara Y., Harada J., Sato H., Yamazaki T., Sakakibara Y., Suiko M., Asada Y., Takahashi Y., Yamamoto K., Fukuyama K., Fukuyama K., Sugishima M., Wada K.
Nature Communications 8 2017年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nature Communications
© The Author(s) 2017.Biliverdin reductase catalyses the last step in haem degradation and produces the major lipophilic antioxidant bilirubin via reduction of biliverdin, using NAD(P)H as a cofactor. Despite the importance of biliverdin reductase in maintaining the redox balance, the molecular details of the reaction it catalyses remain unknown. Here we present the crystal structure of biliverdin reductase in complex with biliverdin and NADP+. Unexpectedly, two biliverdin molecules, which we designated the proximal and distal biliverdins, bind with stacked geometry in the active site. The nicotinamide ring of the NADP+ is located close to the reaction site on the proximal biliverdin, supporting that the hydride directly attacks this position of the proximal biliverdin. The results of mutagenesis studies suggest that a conserved Arg185 is essential for the catalysis. The distal biliverdin probably acts as a conduit to deliver the proton from Arg185 to the proximal biliverdin, thus yielding bilirubin.
DOI： 10.1038/ncomms14397
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Tokumaru M., Adachi F., Toda M., Ito-Inaba Y., Ito-Inaba Y., Yazu F., Hirosawa Y., Sakakibara Y., Suiko M., Kakizaki T., Inaba T.
Plant Physiology 173 ( 1 ) 524 - 535 2017年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plant Physiology
© 2017 American Society of Plant Biologists. All rights reserved.Arabidopsis (Arabidopsis thaliana) GOLDEN2-LIKE (GLK) transcription factors promote chloroplast biogenesis by regulating the expression of photosynthesis-related genes. Arabidopsis GLK1 is also known to participate in retrograde signaling from chloroplasts to the nucleus. To elucidate the mechanism by which GLK1 is regulated in response to plastid signals, we biochemically characterized Arabidopsis GLK1 protein. Expression analysis of GLK1 protein indicated that GLK1 accumulates in aerial tissues. Both tissue-specific and Suc-dependent accumulation of GLK1 were regulated primarily at the transcriptional level. In contrast, norflurazon-or lincomycin-treated gun1-101 mutant expressing normal levels of GLK1 mRNA failed to accumulate GLK1 protein, suggesting that plastid signals directly regulate the accumulation of GLK1 protein in a GUN1-independent manner. Treatment of the glk1glk2 mutant expressing functional GFP-GLK1 with a proteasome inhibitor, MG-132, induced the accumulation of polyubiquitinated GFP-GLK1. Furthermore, the level of endogenous GLK1 in plants with damaged plastids was partially restored when those plants were treated with MG-132. Collectively, these data indicate that the ubiquitin-proteasome system participates in the degradation of Arabidopsis GLK1 in response to plastid signals.
DOI： 10.1104/pp.16.01546
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Yoshimura Toshihiro, Kurogi Katsuhisa, Liu Ming-Cheh, Suiko Masahito, Sakakibara Yoichi
Journal of Electrophoresis 60 ( 1 ) 5 - 14 2016年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本電気泳動学会
<i>S</i>-nitrosylation, a post-translational modification of the thiol group of cysteine residues by nitric oxide (NO), has emerged as a new mode of signal transduction and regulation of protein function. It has recently been shown that <i>S</i>-nitrosylation may result in various protein dysfunctions. However, an improved <i>S</i>-nitrosylation analysis method is needed to achieve high sensitivity and quantitative accuracy. We hypothesized that an analysis method using fluorescence dye could detect <i>S</i>-nitrosylated proteins at a higher sensitivity than that of the conventional method. In this study, we developed a procedure for analyzing <i>S</i>-nitrosylated proteins using CyDye (the CyDye switch method). This CyDye switch method for detecting <i>S</i>-nitrosylated proteins was developed based on the biotin-switch method for analyzing <i>S</i>-nitrosylated proteins. We analyzed NO donor-induced <i>S</i>-nitrosylated proteins in a model protein and at the cellular level. We demonstrated that this CyDye switch method could detect specific <i>S</i>-nitrosylated proteins using SDS-PAGE and mass spectrometry. Our results indicate that the optimized CyDye switch method is suitable for the detection of the post-translational <i>S</i>-nitrosylation of proteins.
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Sulfation of benzyl alcohol by the human cytosolic sulfotransferases (SULTs): a systematic analysis 査読あり
Zhang L., Kurogi K., Kurogi K., Liu M., Liu M., Schnapp A., Williams F., Sakakibara Y., Suiko M., Liu M.
Journal of Applied Toxicology 36 ( 9 ) 1090 - 1094 2016年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Applied Toxicology
Copyright © 2015 John Wiley & Sons, Ltd.The aim of the present study was to identify human cytosolic sulfotransferases (SULTs) that are capable of sulfating benzyl alcohol and to examine whether benzyl alcohol sulfation may occur in cultured human cells as well as in human organ homogenates. A systematic analysis revealed that of the 13 known human SULTs, SULT1A1 SULT1A2, SULTA3, and SULT1B1 are capable of mediating the sulfation of benzyl alcohol. The kinetic parameters of SULT1A1 that showed the strongest benzyl alcohol-sulfating activity were determined. HepG2 human hepatoma cells were used to demonstrate the generation and release of sulfated benzyl alcohol under the metabolic settings. Moreover, the cytosol or S9 fractions of human liver, lung, kidney and small intestine were examined to verify the presence of benzyl alcohol sulfating activity in vivo. Copyright © 2015 John Wiley & Sons, Ltd.
DOI： 10.1002/jat.3268
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Yamamoto A., Yamamoto A., Debrah-Pinamang M., DiModica N., Kurogi K., Kurogi K., Naqvi A., Hui Y., Hui Y., Sakakibara Y., Suiko M., Liu M.
Drug Metabolism Letters 10 ( 3 ) 200 - 205 2016年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Drug Metabolism Letters
© 2016 Bentham Science Publishers.Objective: The aim of the current study was to identify the human cytosolic sulfotransferases (SULTs) that are capable of sulfating clioquinol and iodoquinol, and to verify the presence of clioquinol/ iodoquinol-sulfating activity in human organ homogenates and cultured cells. Method: An established sulfotransferase assay was employed to analyze clioquinol/iodoquinolsulfating activity of thirteen known human SULTs, as well as cytosols of human kidney, liver, lung, and small intestine. Metabolic labeling with [35S]sulfate in the presence of different concentrations of clioquinol/iodoquinol was performed using cultured HepG2 human hepatoma cells and Caco-2 human colon carcinoma cells. Results: A systematic analysis revealed that six of the thirteen known human SULTs, SULT1A1 SULT1A2, SULTA3, SULT1B1, SULT1C4, and SULT1E1 showed considerable clioquinol/ iodoquinol-sulfating activity. Kinetic parameters of the sulfation of clioquinol and iodoquinol by three SULTs, SULT1A1, SULT1A3, and SULT1C4, that showed the strongest clioquinol/iodoquinolsulfating activity were determined. Moreover, clioquinol/iodoquinol-sulfating activity was detected in the cytosol fractions of human liver, lung, kidney, and small intestine. Cultured HepG2 and Caco-2 cells were shown to be capable of sulfating clioquinol/iodoquinol under metabolic conditions. Conclusion: Collectively, these results provided a molecular basis underling the metabolism of clioquinol and iodoquinol through sulfation.
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Human Cytosolic Sulfotransferase SULT1A3 Mediates the Sulfation of Dextrorphan. 査読あり
Yamamoto, A., Kurogi, K., Schiefer, I.T., Liu, M.-Y., Sakakibara, Y., Suiko, M., Liu, M.-C.
Biol. Pharm. Bull. 39 ( 9 ) 1432 - 1436 2016年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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A novel procedure for the assessment of the antioxidant capacity of food components 査読あり
Yoshimura T., Harashima M., Kurogi K., Kurogi K., Suiko M., Suiko M., Liu M., Sakakibara Y., Sakakibara Y.
Analytical Biochemistry 507 7 - 12 2016年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Analytical Biochemistry
© 2016 Elsevier Inc.Carbonylation, an oxidative modification of the amino group of arginine and lysine residues caused by reactive oxygen species, has emerged as a new type of oxidative damage. Protein carbonylation has been shown to exert adverse effects on various protein functions. Recently, the role of food components in the attenuation of oxidative stress has been the focus of many studies. Most of these studies focused on the chemical properties of food components. However, it is also important to determine their effects on protein functions via post-translational modifications. In this study, we developed a novel procedure for evaluating the antioxidant capacity of food components. Hydrogen peroxide (H2O2)-induced protein carbonylation in HL-60 cells was quantitatively analyzed by using fluorescent dyes (Cy5-hydrazide dye and IC3-OSu dye), followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fluorescence determination. Among a panel of food components tested, quinic acid, kaempferol, saponin, squalene, trigonelline, and mangiferin were shown to be capable of suppressing protein carbonylation in HL-60 cells. Our results demonstrated that this fluorescence labeling/SDS-PAGE procedure allows for the detection of oxidative stress-induced protein carbonylation with high sensitivity and quantitative accuracy. This method should be useful for the screening of new antioxidant food components as well as the analysis of their suppression mechanism.
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Sulfate conjugation of daphnetin by the human cytosolic sulfotransferases 査読あり
Han Z., Xi Y., Luo L., Luo L., Zhou C., Zhou C., Kurogi K., Kurogi K., Sakakibara Y., Suiko M., Liu M.
Journal of Ethnopharmacology 189 250 - 252 2016年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Ethnopharmacology
© 2016 Elsevier Ireland Ltd.Ethnopharmacological relevance In Turkey, daphnetin-containing Daphne oleoides is used as a folk medicine for treating rheumatic pain and lumbago. A daphnetin-containing traditional Chinese medicine tablet, named Zushima-Pian, is available in China for treating rheumatoid arthritis. The present study aimed to investigate the metabolism of daphnetin through sulfation in cultured human cells and to identify the human cytosolic sulfotransferase(s) (SULT(s)) that is(are) capable of mediating the sulfation of daphnetin. Materials and methods Cultured HepG2 human hepatoma cells and Caco-2 human colon carcinoma cells were labeled with [35S]sulfate in the presence of different concentrations of daphnetin. Thirteen known human SULTs, previously expressed and purified, as well as cytosols of human kidney, liver, lung, and small intestine, were examined for daphnetin-sulfating activity using an established sulfotransferase assay. Results [35S]sulfated daphnetin was found to be generated and released by HepG2 cells and Caco-2 cells labeled with [35S] sulfate in the presence of daphnetin. Among the 13 known human SULTs, SULT1A1, SULT1A2, SULT1A3, SULT1B1, and SULT1C4 displayed significant sulfating activity toward daphnetin. Of the four human organ samples later tested, small intestine and liver cytosols displayed considerably higher daphnetin-sulfating activity than those of lung and kidney. Conclusion The results derived from the present study showed unequivocally that daphnetin could be sulfated in cultured human cells and by purified human SULT enzymes as well as human organ cytosols. The information obtained provided a basis for further studies on the metabolism of daphnetin through sulfation in vivo.
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Luo L., Luo L., Zhou C., Zhou C., Kurogi K., Sakakibara Y., Suiko M., Liu M.
Xenobiotica 46 ( 7 ) 612 - 619 2016年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Xenobiotica
© 2015 Taylor & Francis.1. This study aimed to investigate the involvement of sulfation in the metabolism of 6-hydroxymelatonin (6-OH-Mel), N-acetylserotonin (NAS) and 4-hydroxyramelteon (4-OH-Ram), and to identify and characterize the human cytosolic sulfotransferases (SULTs) capable of sulfating these drug compounds.2. A systematic analysis using 13 known human SULTs revealed that SULT1A1 displayed the strongest activity in catalyzing the sulfation of 6-OH-Mel and 4-OH-Ram, whereas SULT1C4 exhibited the strongest sulfating-activity towards NAS. pH-dependence and kinetic parameters of these SULT enzymes in mediating the sulfation of respective drug compounds were determined. A metabolic labeling study showed the generation and release of [35S]sulfated 6-OH-Mel, NAS and 4-OH-Ram by HepG2 human hepatoma cells and Caco-2 human colon adenocarcinoma cells labeled with [35S]sulfate in the presence of these drug compounds. Cytosols of human lung, liver, kidney and small intestine were examined to verify the presence of 6-OH-Mel-, NAS- and 4-OH-Ram-sulfating activity in vivo. Of the four human organ samples tested, small intestine and liver cytosols displayed considerably higher 6-OH-Mel-, NAS- and 4-OH-Ram-sulfating activities than those of lung and kidney.3. Collectively, these results provided a molecular basis for the metabolism of 6-OH-Mel, NAS and 4-OH-Ram through sulfation.
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Sulfate Conjugation of Daphnetin by the Human Cytosolic Sulfotransferases. 査読あり
Han, Z., Xi, Y., Luo, L., Zhou, C., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
J。 Ethnopharmacol. S0378-8741 ( 16 ) 30311 - 30317 2016年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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シイタケおよびその加工品による抗酸化ストレス作用 査読あり
近藤知巳,中島有紀子,渡辺朋子,吉山佳世,内田飛香, 黒木勝久,福井敬一,水光正仁,榊原陽一
日本食品科学工学会誌 63 ( 5 ) 199 - 208 2016年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Human cytosolic sulfotransferase SULT1C4 mediates the sulfation of doxorubicin and epirubicin 査読あり
Luo L., Luo L., Zhou C., Zhou C., Hui Y., Hui Y., Kurogi K., Kurogi K., Sakakibara Y., Suiko M., Liu M.
Drug Metabolism and Pharmacokinetics 31 ( 2 ) 163 - 166 2016年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Drug Metabolism and Pharmacokinetics
Copyright © 2016, The Japanese Society for the Study of Xenobiotics.Doxorubicin, an anthracycline, has been reported to be excreted in sulfate conjugated form. The current study aimed to identify the human cytosolic sulfotransferase(s) (SULT(s)) that is(are) capable of sulfating doxorubicin and its analog epirubicin, and to verify whether sulfation of doxorubicin and epirubicin may occur under metabolic conditions. A systematic analysis of thirteen known human SULTs, previously cloned, expressed, and purified, revealed SULT1C4 as the only human SULT capable of sulfating doxorubicin and epirubicin. Cultured HepG2 human hepatoma cells and Caco-2 human colon carcinoma cells were labeled with [35S]sulfate in the presence of different concentrations of doxorubicin or epirubicin. Analysis of spent labeling media showed the generation and release of [35S]sulfated doxorubicin and epirubicin by HepG2 cells and Caco-2 cells. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed the expression of SULT1C4 in both HepG2 cells and Caco-2 cells. These results provided a molecular basis underlying the previous finding that sulfate-conjugated doxorubicin was excreted in the urine of patients treated with doxorubicin.
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Sulfation of benzyl alcohol by the human cytosolic sulfotransferases (SULTs): a systematic analysis. 査読あり
Zhang, L., Kurogi, K., Liu, M.-Y., Schnapp, A.M., Williams, F.E., Sakakibara, Y., Suiko, M., Liu, M.-C.
J. Appl. Toxicol. 2015年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sulphation of acetaminophen by the human cytosolic sulfotransferases: A systematic analysis 査読あり
Yamamoto A., Yamamoto A., Liu M., Kurogi K., Kurogi K., Sakakibara Y., Saeki Y., Suiko M., Liu M.
Journal of Biochemistry 158 ( 6 ) 497 - 504 2015年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Biochemistry
© 2015 The Authors 2015. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.Sulphation is known to be critically involved in the metabolism of acetaminophen in vivo. This study aimed to systematically identify the major human cytosolic sulfotransferase (SULT) enzyme(s) responsible for the sulphation of acetaminophen. A systematic analysis showed that three of the twelve human SULTs, SULT1A1, SULT1A3 and SULT1C4, displayed the strongest sulphating activity towards acetaminophen. The pH dependence of the sulphation of acetaminophen by each of these three SULTs was examined. Kinetic parameters of these three SULTs in catalysing acetaminophen sulphation were determined. Moreover, sulphation of acetaminophen was shown to occur in HepG2 human hepatoma cells and Caco-2 human intestinal epithelial cells under the metabolic setting. Of the four human organ samples tested, liver and intestine cytosols displayed considerably higher acetaminophen-sulphating activity than those of lung and kidney. Collectively, these results provided useful information concerning the biochemical basis underlying the metabolism of acetaminophen in vivo previously reported.
DOI： 10.1093/jb/mvv062
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Sulfation of 6-Gingerol by the Human Cytosolic Sulfotransferases: A Systematic Analysis 査読あり
Luo L., Luo L., Mei X., Mei X., Xi Y., Zhou C., Zhou C., Hui Y., Hui Y., Kurogi K., Sakakibara Y., Suiko M., Liu M.
Planta Medica 82 ( 3 ) 238 - 243 2015年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Planta Medica
© Georg Thieme Verlag KG Stuttgart · New York .Previous studies have demonstrated the presence of the sulfated form of 6-gingerol, a major pharmacologically active component of ginger, in plasma samples of normal human subjects who were administered 6-gingerol. The current study was designed to systematically identify the major human cytosolic sulfotransferase enzyme(s) capable of mediating the sulfation of 6-gingerol. Of the 13 known human cytosolic sulfotransferases examined, six (SULT1A1, SULT1A2, SULT1A3, SULT1B1, SULT1C4, SULT1E1) displayed significant sulfating activity toward 6-gingerol. Kinetic parameters of SULT1A1, SULT1A3, SULT1C4, and SULT1E1 that showed stronger 6-gingerol-sulfating activity were determined. Of the four human organ samples tested, small intestine and liver cytosols displayed considerably higher 6-gingerol-sulfating activity than those of the lung and kidney. Moreover, sulfation of 6-gingerol was shown to occur in HepG2 human hepatoma cells and Caco-2 human colon adenocarcinoma cells under the metabolic setting. Collectively, these results provided useful information relevant to the metabolism of 6-gingerol through sulfation both in vitro and in vivo.
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Sulfation of 6-hydroxymelatonin, N-acetylserotonin and 4-hydroxyramelteon by the human cytosolic sulfotransferases (SULTs). 査読あり
Luo, L., Zhou, C., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
Xenobiotica 46 ( 7 ) 612 - 619 2015年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Hui Y., Hui Y., Luo L., Luo L., Zhang L., Kurogi K., Zhou C., Zhou C., Sakakibara Y., Suiko M., Liu M.
Journal of Pharmacological Sciences 128 ( 3 ) 144 - 149 2015年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Pharmacological Sciences
© 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society. This is an open access article under the CC BY-NC-ND license.Previous studies demonstrated that sulfate conjugation is involved in the metabolism of three commonly used breast cancer drugs, tamoxifen, raloxifene and fulvestrant. The current study was designed to systematically identify the human cytosolic sulfotransferases (SULTs) that are capable of sulfating raloxifene, fulvestrant, and two active metabolites of tamoxifen, afimoxifene and endoxifen. A systematic analysis using 13 known human SULTs revealed SULT1A1 and SULT1C4 as the major SULTs responsible for the sulfation of afimoxifene, endoxifen, raloxifene and fulvestrant. Kinetic parameters of these two human SULTs in catalyzing the sulfation of these drug compounds were determined. Sulfation of afimoxifene, endoxifen, raloxifene and fulvestrant under metabolic conditions was examined using HepG2 human hepatoma cells and MCF-7 breast cancer cells. Moreover, human intestine, kidney, liver, and lung cytosols were examined to verify the presence of afimoxifene/endoxifen/raloxifene/fulvestrant-sulfating activity.
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Abe A., Uchida A., Hoshino Y., Sakakibara Y., Suiko M., Kunitake H.
Acta Horticulturae 1065 1267 - 1274 2015年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Acta Horticulturae
To analyze the protein expression of pollen grains and tubes having self-incompatible reactions in Hyuganatsu (Citrus tamurana Hort. ex Tanaka), we cultured mature pollen and treated it with crude extracts from the styles using a mass or single liquid culture system, and we collected pollen grains and tubes for proteome analysis. The relative expression of each identified protein was quantified by an imager and revealed as a value relative to the pollen grain. Eleven identified proteins were remarkably up-regulated (above 1.2-fold) or down-regulated (under 0.8-fold) in treatment with self-incompatible reactions. Eight of these proteins were predicted to be self-incompatible-related proteins with the reported functions. In this study, F-box protein was identified as showing minimum expression in the treatment with crude extracts from styles of Hyuganatsu (self-incompatible reactions). The S-locus gene product expressed in pollen has been identified as an F-box protein in Solanaceae and Rosaceae. It is not clear whether a mechanism similar to that of Solanaceae and Rosaceae exists in the self-incompatible reaction in Hyuganatsu; however, the reduced expression of F-box protein may induce a self-incompatible reaction.
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タンパク質蛍光標識技術を用いた<i>S-</i>ニトロシル化タンパク質の解析法開発 査読あり
芳村 俊広, 黒木 勝久, 水光 正仁, 榊原 陽一
電気泳動 59 ( 2 ) 123 - 125 2015年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japanese Electrophoresis Society
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Nagahama K., Eto N., Eto N., Shimojo T., Kondoh T., Kondoh T., Nakahara K., Sakakibara Y., Sakakibara Y., Fukui K., Suiko M., Suiko M.
Bioscience, Biotechnology and Biochemistry 79 ( 8 ) 1327 - 1336 2015年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
© 2015 Japan Society for Bioscience, Biotechnology, and Agrochemistry.Natural killer (NK) cells play a key role in innate immune defense against infectious disease and cancer. A reduction of NK activity is likely to be associated with increased risk of these types of disease. In this study, we investigate the activation potential of kumquat pericarp acetone fraction (KPAF) on NK cells. It is shown to significantly increase IFN-γ production and NK cytotoxic activity in human KHYG-1 NK cells. Moreover, oral administration of KP-AF significantly improves both suppressed plasma IFN-γ levels and NK cytotoxic activity per splenocyte in restraint-stressed mice. These results indicate that raw kumquat pericarp activates NK cells in vitro and in vivo. To identify the active constituents, we also examined IFN-γ production on KHYG-1 cells by the predicted active components. Only β-cryptoxanthin increased IFN-γ production, suggesting that NK cell activation effects of KP-AF may be caused by carotenoids such as β-cryptoxanthin.
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Yamasaki M., Nishimura M., Sakakibara Y., Suiko M., Morishita K., Nishiyama K.
Food and Function 5 ( 11 ) 2842 - 2849 2014年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Food and Function
© 2014 the Partner Organisations.Here, we examined the effect of tocotrienols (T3) on the growth of adult T-cell leukemia (ATL) cells. All three forms (β-, γ-, and δ-T3) inhibited cell proliferation in a dose-dependent manner; δ-T3 showed the strongest growth-inhibitory effect. δ-T3 increased the G1, G2/M, and subG1 populations and induced internucleosomal DNA fragmentation. δ-T3 treatment also increased the levels of cleaved caspase-3, -6, -7, -9, and poly-ADP ribose polymerase (PARP), and this was accompanied by downregulation of Bcl-2, Bcl-xL, and XIAP. Moreover, δ-T3 decreased nuclear p65 NF-κB levels, indicating downregulation of NF-κB activity. This cytotoxic effect of δ-T3 was abrogated by squalene (SQL) but not mevalonate (MVL), farnesyl diphosphate (FPP), geranylgeranyl diphosphate (GGPP), or cholesterol (CL). δ-T3 decreased intracellular SQL levels, and inhibition of de novo cholesterol synthesis did not affect the action of SQL. Furthermore, δ-T3 significantly decreased farnesyl-diphosphate farnesyltransferase 1 (FDFT1) expression. Taken together, it is evident that δ-T3, due to its ability to potently induce apoptosis via the depletion of intracellular SQL, shows the potential to be considered a therapeutic agent in patients with ATL.
DOI： 10.1039/c4fo00635f
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Kurogi K., Chepak A., Hanrahan M., Liu M., Sakakibara Y., Suiko M., Liu M.
European Journal of Pharmaceutical Sciences 62 40 - 48 2014年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：European Journal of Pharmaceutical Sciences
The current study was designed to examine the sulfation of eight opioid drugs, morphine, hydromorphone, oxymorphone, butorphanol, nalbuphine, levorphanol, nalorphine, and naltrexone, in HepG2 human hepatoma cells and human organ samples (lung, liver, kidney, and small intestine) and to identify the human SULT(s) responsible for their sulfation. Analysis of the spent media of HepG2 cells, metabolically labeled with [35S]sulfate in the presence of each of the eight opioid drugs, showed the generation and release of corresponding [35S]sulfated derivatives. Five of the eight opioid drugs, hydromorphone, oxymorphone, butorphanol, nalorphine, and naltrexone, appeared to be more strongly sulfated in HepG2 cells than were the other three, morphine, nalbuphine, and levorphanol. Differential sulfating activities toward the opioid drugs were detected in cytosol or S9 fractions of human lung, liver, small intestine, and kidney, with the highest activities being found for the liver sample. A systematic analysis using eleven known human SULTs and kinetic experiment revealed SULT1A1 as the major responsible SULTs for the sulfation of oxymorphone, nalbuphine, nalorphine, and naltrexone, SULT1A3 for the sulfation of morphine and hydromorphone, and SULT2A1 for the sulfation of butorphanol and levorphanol. Collectively, the results obtained imply that sulfation may play a significant role in the metabolism of the tested opioid drugs in vivo. © 2014 Elsevier Inc. All rights reserved.
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Identification of a novel flavonoid glycoside sulfotransferase in Arabidopsis thaliana 査読あり
Hashiguchi T., Hashiguchi T., Sakakibara Y., Sakakibara Y., Shimohira T., Shimohira T., Kurogi K., Kurogi K., Yamasaki M., Yamasaki M., Nishiyama K., Nishiyama K., Akashi R., Liu M., Suiko M., Suiko M.
Journal of Biochemistry 155 ( 2 ) 91 - 97 2014年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Biochemistry
The discovery of sulfated flavonoids in plants suggests that sulfation may play a regulatory role in the physiological functions of flavonoids. Sulfation of flavonoids is mediated by cytosolic sulfotransferases (SULTs), which utilize 3′-phosphoadenosine 5′-phosphosulfate (PAPS) as the sulfate donor. A novel SULT from Arabidopsis thaliana, designated AtSULT202B7 (AGI code: At1g13420), was cloned and expressed in Escherichia coli. Using various compounds as potential substrates, we demonstrated, for the first time, that AtSULT202B7 displayed sulfating activity specific for flavonoids. Intriguingly, the recombinant enzyme preferred flavonoid glycosides (e.g. kaempferol-3- glucoside and quercetin-3-glucoside) rather than their aglycone counterparts. Among a series of hydroxyflavones tested, AtSULT202B7 showed the enzymatic activity only for 7-hydroxyflavone. pH-dependency study showed that the optimum pH was relatively low (pH 5.5) compared with those (pH 6.0-8.5) previously reported for other isoforms. Based on the comparison of high performance (pressure) liquid chromatography (HPLC) retention times between sulfated kaempferol and the deglycosylated product of sulfated kaempferol-3-glucoside, the sulfation site in sulfated kaempferol-3-glucoside appeared to be the hydroxyl group of the flavonoid skeleton. In addition, by using direct infusion mass spectrometry, it was found that the sulfated product had one sulfonate group within the molecule. These results indicated that AtSULT202B7 functions as a flavonoid glycoside 7-sulfotransferase. © 2013 The Authors.
DOI： 10.1093/jb/mvt102
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Sulfation of phenylephrine by the human cytosolic sulfotransferases 査読あり
Yamamoto A., Yamamoto A., Kim J., Liu M., Kurogi K., Kurogi K., Sakakibara Y., Suiko M., Liu M.
Drug Metabolism Letters 8 ( 2 ) 96 - 100 2014年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Drug Metabolism Letters
© 2014 Bentham Science Publishers.Previous studies had demonstrated that sulfation constituted a major pathway for the metabolism of phenylephrine in vivo. The current study was designed to identify the major human SULT(s) responsible for the sulfation of phenylephrine. Of the twelve human SULTs analyzed, SULT1A3 displayed the strongest sulfating activity toward phenylephrine. The enzyme exhibited a pH optimum spanning 7 – 10.5. Kinetic analysis revealed that SULT1A3-mediated sulfation of phenylephrine occurred in the same order of magnitude compared with that previously reported for SULT1A3-mediated sulfation of dopamine. Moreover, sulfation of phenylephrine was shown to occur in HepG2 cells under metabolic setting. Collectively, these results provided useful information concerning the biochemical basis underlying the metabolism of phenylephrine in vivo as previously reported.
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Yamasaki M., Iwase M., Kawano K., Sakakibara Y., Suiko M., Ikeda M., Nishiyama K.
Journal of Clinical Biochemistry and Nutrition 54 ( 1 ) 18 - 25 2014年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Clinical Biochemistry and Nutrition
Our previous study showed α-lipoic and (LA) downregulated cell surface β1-integrin expression of v-H-ras-transformed derivative of rat fibroblast with amelioration of their malignant phenotype. Here, we evaluated the ameliorating effect of LA on the malignant characters in H-ras-transformed bladder cancer cells. H-ras mutated bladder cancer line, T24 cells were incubated with LA to evaluate the inhibitory effect on proliferation, migration, invasion and β1-integrin expression. Fluorescence staining of F-actin and western blotting analyses of the related signaling pathways were also performed. LA inhibited the proliferation of T24 cells. Cell adhesion to collagen IV and fibronectin was strikingly inhibited by LA treatment accompanied by downregulation of cell surface but not whole cell β1-integrin expression. LA clearly inhibited cell migration and invasion of T24 cells, which were mimicked by extra-cellular signal-regulated kinase (ERK) and Akt pathway inhibition. Actually, LA significantly downregulated the phosphorylated ERK and Akt levels. Moreover, LA downregulated phosphorylated focal adhesion kinase level with disappearance of stress fiber formation. Finally, although LA induced the internalization of cell surface β1-integrin, disruption of the raft did not affect the action of LA. Taken together, LA is a promising agent to improve malignant character of bladder cancer cells through regulation of cellular β1-integrin localization. ©2014 JCBN.
DOI： 10.3164/jcbn.13-57
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Nagahama K., Eto N., Sakakibara Y., Matsusita Y., Sugamoto K., Morishita K., Suiko M.
Journal of Functional Foods 6 ( 1 ) 356 - 366 2014年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Functional Foods
Adult T-cell leukemia/lymphoma (ATLL) is an aggressive disease caused by human T-cell lymphotropic virus type 1 (HTLV-1). As ATLL develops over many years, daily diet may play an important role in its occurrence. In this study, we investigated the growth inhibitory effect of extracts of blueberry leaf fractions on HTLV-1-associated cell lines. The most effective fraction contained highly oligomeric proanthocyanidins (OPA) with a mean degree of polymerization of 3.1. Cell growth inhibition by the OPA fraction was higher in all HTLV-1-associated cell lines than in the MOLT-4 HTLV-1-negative T-cells. It inhibited growth of the HTLV-1-infected T-cell line MT2 and ATLL-derived cell line Su9T01 by inducing apoptosis and G2/M cell cycle arrest. OPA fraction-induced apoptosis of HTLV-1-associated cells required death receptor-mediated caspase-dependent pathways. Treatment-induced G2/M arrest resulted from down-regulation of cyclin B1 and cdc2. The blueberry leaf OPA fraction may be a source of novel compounds for reducing the risk of developing ATLL. © 2013 Elsevier Ltd.
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Yamasaki M., Soda S., Sakakibara Y., Suiko M., Nishiyama K.
Bioscience, Biotechnology and Biochemistry 78 ( 11 ) 1939 - 1942 2014年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
© 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry.Here, we show that cell surface β1-integrin expression, cell adhesion to fibronectin, migration, and invasion were all significantly inhibited by α-lipoic acid. These effects were not observed when cells were treated with dihydrolipoic acid or caprylic acid. These data reveal that the 1,2-dithiolane structure plays an important role in the action of α-lipoic acid.
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Induction of apoptotic cell death in HL-60 cells by jacaranda seed oil derived fatty acids 査読あり
Yamasaki M., Motonaga C., Yokoyama M., Ikezaki A., Kakihara T., Hayasegawa R., Yamasaki K., Sakono M., Sakakibara Y., Suiko M., Nishiyama K.
Journal of Oleo Science 62 ( 11 ) 925 - 932 2013年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oleo Science
Various fatty acids are attracting considerable interest for their anticancer effects. Among them, fatty acids containing conjugated double bonds show one of the most potent cytotoxic effects on cancer cells. Here, we focused on the cancer cell killing activity of jacaranda seed oil. The seed oil of jacaranda harvested from Miyazaki in Japan contained 30.9% cis-8, trans-10, cis-12 octadecatrienoic acid, called jacaric acid (JA). Fatty acid prepared from this oil (JFA) and JA strongly induced cell death in human leukemia HL-60 cells. On the other hand, linoleic acid and trans-10, cis-12 conjugated linoleic acid (<10 μM) did not affect cell proliferation and viability. An increase in the sub-G1 population and internucleosomal fragmentation of DNA was observed in JA- and JFA-treated cells, indicating induction of apoptotic cell death. Finally, the cytotoxic effects of JA and JFA were completely abolished by α-tocopherol. Taken together, these data suggest that jacaranda seed oil has potent apoptotic activity in HL-60 cells through induction of oxidative stress. © 2013 by Japan Oil Chemists' Society.
DOI： 10.5650/jos.62.925
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Kurogi K., Kurogi K., Liu T., Sakakibara Y., Suiko M., Liu M.
Drug Metabolism Reviews 45 ( 4 ) 431 - 440 2013年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Drug Metabolism Reviews
In recent years, zebrafish has emerged as a useful animal model for biomedical research. The deciphering of the zebrafish genome has revealed that many of the enzymes involved in the metabolism of endogenous compounds and xenobiotics are conserved between zebrafish and humans. This review summarizes the information currently available concerning the zebrafish cytosolic sulfotransferases (SULTs), a group of phase II enzymes that have been proposed to be involved in the regulation and homeostasis of key endogenous compounds and the detoxification of xenobiotics. To date, 20 zebrafish SULTs that fall into six major SULT gene families have been identified. Of the 20 SULTs 18 have been cloned, expressed, purified and characterized. These zebrafish SULTs were shown to exhibit differential substrate specificities for endogenous compounds such as monoamine transmitters, steroid/thyroid hormones and bile salts, as well as xenobiotics including environmental toxicants and drugs. These findings provide a foundation for using zebrafish as a model for investigating further the physiological, pharmacological, and toxicological involvement of the SULTs. © 2013 Informa Healthcare USA, Inc.
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Yamasaki M., Mine Y., Nishimura M., Fujita S., Sakakibara Y., Suiko M., Morishita K., Nishiyama K.
Cell Biology International 37 ( 7 ) 742 - 747 2013年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cell Biology International
We have shown that genistein inhibits the growth of adult T-cell leukemia (ATL) cells in vitro and in vivo, and this leads to pronounced G2/M arrest. This report shows that genistein induces apoptotic death in ATL cells. Although the pan-caspase inhibitor, Z-VAD-fmk, did not inhibit genistein-induced apoptosis, release of apoptosis-inducing factor (AIF) into the cytosol occurred. Poly-ADP ribose polymerase inhibition also abrogated genistein-induced apoptosis. Genistein decreased nuclear p65 translocation and IkBα phosphorylation, and downregulated the anti-apoptotic proteins, XIAP, cIAP and survivin, NF-kBresponsive gene products. Thus, genistein is a promising agent for ATL that induces caspase-independent apoptosis through inhibition of the NF-kB pathway. © 2013 International Federation for Cell Biology.
DOI： 10.1002/cbin.10104
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Identification and characterization of a novel kaempferol sulfotransferase from Arabidopsis thaliana 査読あり
Hashiguchi T., Hashiguchi T., Sakakibara Y., Sakakibara Y., Hara Y., Shimohira T., Kurogi K., Kurogi K., Akashi R., Akashi R., Liu M., Suiko M., Suiko M.
Biochemical and Biophysical Research Communications 434 ( 4 ) 829 - 835 2013年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochemical and Biophysical Research Communications
In plants, flavonoids have been shown to be subjected to conjugation modifications such as glycosylation, methylation, and sulfation. Among these modifications, sulfation is known as an important pathway in the regulation of the levels of endogenous compounds such as steroids. Although a large variety of flavonoid sulfates also exist in plants, the detailed biochemical characterization of Arabidopsis thaliana sulfotransferases (AtSULTs) remains to be fully clarified. We report here that uncharacterized AtSULT202E1 (AGI code: At2g03770), a SULT202E subfamily member, shows the sulfating activity toward flavonoids. The general characteristics of the enzyme were studied on the optimum temperature and pH, the effect of divalent cations, and the thermal stability with kaempferol as substrate. A comparative analysis of the sulfation of flavonoids by AtSULT202E1, AtSULT202B1 and AtSULT202A1 revealed that three AtSULTs have differential substrate specificities. Surprisingly, 3-hydroxyflavone was sulfated only by AtSULT202A1 while 7-hydroxyflavone was highly sulfated by AtSULT202E1 and AtSULT202B1. These results indicate that flavonols might be sulfated in a position specific manner. In conclusion, our studies indicate that a novel AtSULT202E1 has the sulfating activity toward flavonoids together with AtSULT202B1 and AtSULT202A1. The existence of three flavonoid sulfotransferases in A. thaliana suggests that sulfation of flavonoids have an important role in regulation of their functions. © 2013 Elsevier Inc.
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Teramoto T., Fujikawa Y., Kawaguchi Y., Kurogi K., Soejima M., Adachi R., Nakanishi Y., Mishiro-Sato E., Liu M., Sakakibara Y., Suiko M., Kimura M., Kakuta Y.
Nature Communications 4 2013年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nature Communications
Post-translational protein modification by tyrosine sulfation has an important role in extracellular protein-protein interactions. The protein tyrosine sulfation reaction is catalysed by the Golgi enzyme called the tyrosylprotein sulfotransferase. To date, no crystal structure is available for tyrosylprotein sulfotransferase. Detailed mechanism of protein tyrosine sulfation reaction has thus remained unclear. Here we present the first crystal structure of the human tyrosylprotein sulfotransferase isoform 2 complexed with a substrate peptide (C4P5Y3) derived from complement C4 and 3′- phosphoadenosine-5′-phosphate at 1.9 Å resolution. Structural and complementary mutational analyses revealed the molecular basis for catalysis being an S N 2-like in-line displacement mechanism. Tyrosylprotein sulfotransferase isoform 2 appeared to recognize the C4 peptide in a deep cleft by using a short parallel β-sheet type interaction, and the bound C4P5Y3 forms an L-shaped structure. Surprisingly, the mode of substrate peptide recognition observed in the tyrosylprotein sulfotransferase isoform 2 structure resembles that observed for the receptor type tyrosine kinases. © 2013 Macmillan Publishers Limited. All rights reserved.
DOI： 10.1038/ncomms2593
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Ethanol sulfation by the human cytosolic sulfotransferases: a systematic analysis. 査読あり
Kurogi K, Davidson G, Mohammed YI, Williams FE, Liu MY, Sakakibara Y, Suiko M, Liu MC.
Biol Pharm Bull. 35 ( 12 ) 2180 - 2185 2012年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Nagatomo T., Matsuyama T., Ikeho Y., Kato E., Nishiyama K., Sakakibara Y., Suiko M., Nishiyama K.
Journal of Oleo Science 61 ( 9 ) 491 - 496 2012年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oleo Science
The anti-cancer effects of various fatty acids are drawing a lot of attention. To determine whether different fatty acids affect the hypoxic response of liver cancer cells, we examined the effects of various fatty acids on the stabilization of the hypoxia-inducible factor (HIF)-1α protein in the HepG2 human hepatocellular carcinoma (HCC) cell line under condition containing 1% O 2. Of the fatty acids examined, only 9cis, 11trans (c9, t11)-conjugated linoleic acid (CLA) and 10trans, 12cis (t10, c12)-CLA inhibited hypoxia-induced HIF-1α stabilization. In addition, HIF-1α prolyl hydroxylase or proteasome inhibition abrogated the effects of c9, t11- and t10, c12-CLA. Moreover, c9, t11- and t10, c12-CLA significantly inhibited cell proliferation and induced apoptotic cell death under hypoxia. This is the first study showing that c9, t11- and t10, c12-CLA inhibit the hypoxic response in HCC cells. ©2012 by Japan Oil Chemists' Society.
DOI： 10.5650/jos.61.491
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Kurogi K., Alazizi A., Liu M., Sakakibara Y., Suiko M., Sugahara T., Liu M.
Biochemical Pharmacology 84 ( 9 ) 1186 - 1195 2012年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochemical Pharmacology
Catecholic drugs had been reported to be metabolized through conjugation reactions, particularly methylation and sulfation. Whether and how these two Phase II conjugation reactions may occur in a concerted manner, however, remained unclear. The current study was designed to investigate the methylation and/or sulfation of five catecholic drugs. Analysis of the spent media of HepG2 cells metabolically labeled with [35S]sulfate in the presence of individual catecholic drugs revealed the presence of two [35S] sulfated metabolites for dopamine, epinephrine, isoproterenol, and isoetharine, but only one [35S]sulfated metabolite for apomorphine. Further analyses using tropolone, a catechol O-methyltransferase (COMT) inhibitor, indicated that one of the two [35S]sulfated metabolites of dopamine, epinephrine, isoproterenol, and isoetharine was a doubly conjugated (methylated and sulfated) product, since its level decreased proportionately with increasing concentrations of tropolone added to the labeling media. Moreover, while the inhibition of methylation resulted in a decrease of the total amount of [ 35S]sulfated metabolites, sulfation appeared to be capable of compensating the suppressed methylation in the metabolism of these four catecholic drugs. A two-stage enzymatic assay showed the sequential methylation and sulfation of dopamine, epinephrine, isoproterenol, and isoetharine mediated by, respectively, the COMT and the cytosolic sulfotransferase SULT1A3. Collectively, the results from the present study implied the concerted actions of the COMT and SULT1A3 in the metabolism of catecholic drugs. © 2012 Elsevier Inc. All rights reserved.
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Sulfation of ractopamine and salbutamol by the human cytosolic sulfotransferases. 査読あり
Ko K, Kurogi K, Davidson G, Liu MY, Sakakibara Y, Suiko M, Liu MC.
J Biochem. 152 ( 3 ) 275 - 283 2012年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Uchida A., Sassa H., Takenaka S., Takenaka S., Sakakibara Y., Suiko M., Kunitake H.
Plant OMICS 5 ( 4 ) 320 - 325 2012年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plant OMICS
The differences between stylar protein of the Japanese pear (Pyrus pyrifolia (Burm.f.)) cultivars 'Kosui' (S4S5) and 'Kikusui' (S2S4) were compared by two-dimensional difference gel electrophoresis (2-D DIGE), and were labelled and visualized with different fluorescent dyes (IC3-OSu, IC5-OSu) on a single 2-D gel. The individual different expressed proteins spots were subjected to identification. The proteins were analyzed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF/MS) to identify proteins related to gametophytic self-incompatibility (GSI). S4-RNase and thaumatin-like protein 1 were successfully detected as expected in the pistils of 'Kosui' and 'Kikusui'. S5-RNase was also detected in the pistils of 'Kosui'. However, we could not detect S2-RNase in 'Kikusui' in this study, possibly because the level of expression of S2-RNase might be minuscule, or the estimated isoelectric point (pI) of S2-RNase (pI:9.26) was more basic than S4-RNase (pI: 9.17) and S5-RNase (pI: 9.01). These results indicate that proteomic studies are effective tools for detection of the expected proteins and might be helpful for finding the unknown key proteins related to the mechanism of self-incompatibility (SI) in many other SI plants.
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Yamasaki M., Iwase M., Kawano K., Sakakibara Y., Suiko M., Nishiyama K.
Bioscience, Biotechnology and Biochemistry 76 ( 6 ) 1177 - 1181 2012年6月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
We determined the effects of apocynin, a representative inhibitor of NADPH oxidase, on the proliferative and adhesive properties of 3Y1 rat fibroblasts and the 3Y1 v-H-ras-transformed derivative, HR-3Y1-2. Apocynin inhibited the proliferation of HR-3Y1-2 but not 3Y1 cells at 10μM and 100μM. Apocynin also decreased the intracellular reactive oxygen species (ROS) level in HR-3Y1-2 but not 3Y1 cells. We also evaluated the effects of apocynin on cell adhesion to fibronectin and found decreased adhesion of HR-3Y1-2 cells to fibronectincoated plates. Our results indicate that apocynin selectively down-regulated β1-integrin cell surface expression on the HR-3Y1-2 cells. It also inhibited the migration and invasion of these cells. These data suggest that reducing the production of NADPH oxidasemediated ROS could be an effective means for ameliorating the abnormal growth, adhesion and motility of v-H-ras-transformed cells.
DOI： 10.1271/bbb.120061
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Apocynin Selectively Inhibits Proliferation, Adhesion to Fibronectin of v-H-ras-transformed 3Y1 Cells. 査読あり
4) Yamasaki, M., Iwase, M., Kawano, K., Sakakibara, Y., Suiko, M., and K. Nishiyama.
Biosci. Biotechnol. Biochem. 76 1177 - 1181 2012年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sulfation of Buprenorphine, Pentazocine, and Naloxone by Human Cytosolic Sulfotransferases 査読あり
Kurogi, K., Chen, M., Lee, Y., Shi, B., Yang, T., Liu, M.-Y., Sakakibara, Y., Suiko, M., Liu, M.-C.
Drug Metab. Lett. 6 109 - 115 2012年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Identification and characterization of zebrafish SULT1 ST9, SULT3 ST4, and SULT3 ST5 査読あり
Mohammed Y., Kurogi K., Shaban A., Xu Z., Liu M., Williams F., Sakakibara Y., Suiko M., Bhuiyan S., Liu M.
Aquatic Toxicology 112-113 11 - 18 2012年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Aquatic Toxicology
By searching the GenBank database, we identified sequences encoding three new zebrafish cytosolic sulfotransferases (SULTs). These three new zebrafish SULTs, designated SULT1 ST9, SULT3 ST4, and SULT3 ST5, were cloned, expressed, purified, and characterized. SULT1 ST9 appeared to be mostly involved in the metabolism and detoxification of xenobiotics such as β-naphthol, β-naphthylamine, caffeic acid and gallic acid. SULT3 ST4 showed strong activity toward endogenous compounds such as dehydroepiandrosterone (DHEA), pregnenolone, and 17β-estradiol. SULT3 ST5 showed weaker, but significant, activities toward endogenous compounds such as DHEA and corticosterone, as well as xenobiotics including mestranol, β-naphthylamine, β-naphthol, and butylated hydroxyl anisole (BHA). pH-dependency and kinetic constants of these three enzymes were determined with DHEA, β-naphthol, and 17β-estradiol as substrates. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to examine the expression of these three new zebrafish SULTs at different developmental stages during embryogenesis, through larval development, and on to maturity. © 2012 Elsevier B.V..
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Yamasaki M., Iwase M., Kawano K., Sakakibara Y., Suiko M., Nishiyama K.
Journal of Clinical Biochemistry and Nutrition 50 ( 3 ) 234 - 240 2012年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Clinical Biochemistry and Nutrition
Here, we focused on the effects of racemic α-lipoic acid on pro-liferation and adhesion properties of 3Y1 rat fibroblasts and the v-H-ras-transformed derivative, HR-3Y1-2 cells. Racemic α-lipoic acid inhibited proliferation of HR-3Y1-2 but not 3Y1 cells at 0.3 and 1.0 mM. R-(+)-α-lipoic acid also inhibited proliferation of HR-3Y1-2 cells equivalent to that of racemic α-lipoic acid. In addition, racemic α-lipoic acid decreased intracellular reactive oxygen species levels in HR-3Y1 cells but not 3Y1 cells. Next, we evaluated the effects of racemic α-lipoic acid on cell adhesion to fibronectin. The results indicated that racemic α-lipoic acid decreased adhesive ability of HR-3Y1-2 cells to fibronectin-coated plates. As blocking antibody experiment revealed that β1-integrin plays a key role in cell adhesion in this experimental system, the effects of racemic α-lipoic acid on the expression of β1-integrin were examined. The results indicated that racemic α-lipoic acid selectively downregulated the expression of cell surface β1-integrin expression in HR-3Y1-2 cells. Intriguingly, exogenous hydrogen peroxide upregulated cell surface β1-integrin expression in 3Y1 cells. Taken together, these data suggest that reduction of intracellular reactive oxygen species levels by α-lipoic acid could be an effective means of ameliorating abnormal growth and adhesive properties in v-H-ras transformed cells. ©2012 JCBN.
DOI： 10.3164/jcbn.11-67
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Uchida A., Takenaka S., Takenaka S., Sakakibara Y., Kurogi S., Honsho C., Sassa H., Suiko M., Kunitake H.
Journal of the Japanese Society for Horticultural Science 81 ( 2 ) 150 - 158 2012年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of the Japanese Society for Horticultural Science
In Citrus, self-incompatibility (SI) regulation is gametophytic, and this phenomenon is an economically critical problem for some Citrus cultivars without high parthenocarpic ability. Few molecular biological studies of SI in Citrus have been performed, and the molecular mechanism of SI has not been clarified. To investigate the effect of different stages of style development on pollen tube behavior, flower buds of self-incompatible 'Hyuganatsu' (Citrus tamurana hort. ex Tanaka) were histologically assayed. When the flower buds were observed 168 hours after pollination, pollen tubes in the self-pollinated flower buds 1 and 3 days before anthesis (DBA) were arrested in the upper part of the styles, while those in flower buds self-pollinated 5 DBA reached the base of the styles. These results revealed that SI in 'Hyuganatsu' has not yet occurred in the flower buds 5 DBA, but generated in the flower buds 3 DBA. To search SI-related pistil proteins in Citrus, we profiled a number of protein expressions in 'Hyuganatsu' styles of 1, 3, and 5 DBA by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. One-hundred thirty-eight protein spots were significantly different in abundance among the three stages, and 17 up-regulated and 26 down-regulated protein spots could be identified. Among the 17 up-regulated proteins, nine up-regulated proteins exhibited the expression pattern of 1 DBA ≥ 3 DBA > 5 DBA, a pattern which reflected the transmission from SC to SI, evaluated by pollen tube growth. BLASTP homology search against the peptide sequences of these proteins was carried out and predicted the proteins related to the maintenance of cell shape, the signaling of pollen tube growth, the flavonol biosynthesis, the responses to various stresses, photosynthesis, and the methionine metabolic process. Among the nine proteins, some may be the SI-related pistil proteins; however, it was not possible to determine the SI-related key protein of the style in Citrus. © 2012.
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Alpha Lipoic Acid Selectively Inhibits Proliferation and Adhesion to Fibronectin of v-H-ras-transformed 3Y1 Cells. 査読あり
Yamasaki, M., Iwase, M., Kawano, K., Sakakibara, Y., Suiko, M., and K. Nishiyama.
J. Clin. Biochem. Nutr 50 234 - 240 2012年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Conjugated linoleic acids inhibit hypoxia inducible factor-1alpha stabilization under hypoxic condition in human hepatocellular carcinoma cells. 査読あり
Yamasaki, M., Nagatomo, T., Matsuyama, T., Ikeho, Y., Kato, E., Nishiyama, K., Sakakibara, Y., Suiko, M., Nishiyama, N.
J. Oleo Sci. 61 ( 9 ) 491 - 496 2012年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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自家不和合性の'ヒュウガナツ'（<i>Citrus tamurana</i> hort. ex Tanaka）花柱の異なる発達ステージにおける発現タンパク質の網羅的解析
内田 飛香, 竹中 聡, 榊原 陽一, 黒木 重文, 本勝 千歳, 佐々 英徳, 水光 正仁, 國武 久登
Journal of the Japanese Society for Horticultural Science 81 ( 2 ) 150 - 158 2012年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：一般社団法人 園芸学会
これまでにカンキツにおける自家不和合性の分子生物学的研究が行われてきたが，そのメカニズムについては未だ明らかになっていない．花柱の成育ステージの違いが引き起こす花粉管の挙動の経時的変化を調査するため，自家不和合性品種'ヒュウガナツ'の花蕾の組織学的調査を行った．自家受粉 168 時間後において，開花 1 日前と 3 日前に受粉した花蕾の花粉管は花柱上部に停滞していた．一方，開花 5 日前に受粉した花蕾では花粉管が花柱基部まで到達していた．これらの結果，'ヒュウガナツ'の開花 5 日前の花蕾においては自家不和合性反応は生じていないが，開花 3 日前の花蕾においては自家不和合性反応が生じていることが明らかとなった．そこで，カンキツにおける自家不和合性に関連する雌ずいタンパク質を探索するため，'ヒュウガナツ'の開花 1，3，5 日前の花蕾における発現タンパク質のプロファイリングを二次元電気泳動法と MALDI-TOF/MS 法を用いて行った．3 つの異なるステージにおいて，138 個のタンパク質の発現量に有意な差が観察され，そのうち 17 個の発現量が上昇しているタンパク質と，26 個の発現量が減少しているタンパク質が同定された．17 個の発現量が上昇していたタンパク質のうち，9 個のタンパク質が示す開花 1 日前 ≧ 開花 3 日前 ＞ 開花 5 日前の発現パターンが，花粉管の挙動に基づく自家和合性から自家不和合性への移行を反映していると思われた．BLASTP によるペプチド配列の相同性探索により，これらは細胞形成の維持や花粉管伸長におけるシグナル，フラボノイド生合成系，ストレス応答，光合成系やメチオニン代謝経路に関するタンパク質であると推定された．これら 9 個のタンパク質のうち，いずれかが自家不和合性と関連を持つ花柱タンパク質である可能性があると考えられた．しかしながら，これらの中からカンキツにおける自家不和合性反応の鍵となるタンパク質を明らかにすることはできなかった．<br>
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A comparative study of the sulfation of bile acids and a bile alcohol by the Zebra danio (Danio rerio) and human cytosolic sulfotransferases (SULTs).
Kurogi K, Krasowski MD, Injeti E, Liu MY, Williams FE, Sakakibara Y, Suiko M, Liu MC.
J. Steroid Biochem. Mol. Biol. 127 ( 3-5 ) 307 - 314 2011年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Kurogi K., Krasowski M., Injeti E., Liu M., Williams F., Sakakibara Y., Suiko M., Liu M.
Journal of Steroid Biochemistry and Molecular Biology 127 ( 3-5 ) 307 - 314 2011年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Steroid Biochemistry and Molecular Biology
The current study was designed to examine the sulfation of bile acids and bile alcohols by the Zebra danio (Danio rerio) SULTs in comparison with human SULTs. A systematic analysis using the fifteen Zebra danio SULTs revealed that SULT3 ST2 and SULT3 ST3 were the major bile acid/alcohol-sulfating SULTs. Among the eleven human SULTs, only SULT2A1 was found to be capable of sulfating bile acids and bile alcohols. To further investigate the sulfation of bile acids and bile alcohols by the two Zebra danio SULT3 STs and the human SULT2A1, pH-dependence and kinetics of the sulfation of bile acids/alcohols were analyzed. pH-dependence experiments showed that the mechanisms underlying substrate recognition for the sulfation of lithocholic acid (a bile acid) and 5α-petromyzonol (a bile alcohol) differed between the human SULT2A1 and the Zebra danio SULT3 ST2 and ST3. Kinetic analysis indicated that both the two Zebra danio SULT3 STs preferred petromyzonol as substrate compared to bile acids. In contrast, the human SULT2A1 was more catalytically efficient toward lithocholic acid than petromyzonol. Collectively, the results imply that the Zebra danio and human SULTs have evolved to serve for the sulfation of, respectively, bile alcohols and bile acids, matching the cholanoid profile in these two vertebrate species. © 2010 Elsevier Ltd. All rights reserved.
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Enzymatic sulfation of tocopherols and tocopherol metabolites by human cytosolic sulfotransferases 査読あり
Hashiguchi T., Hashiguchi T., Kurogi K., Kurogi K., Sakakibara Y., Sakakibara Y., Yamasaki M., Yamasaki M., Nishiyama K., Yasuda S., Liu M., Suiko M., Suiko M.
Bioscience, Biotechnology and Biochemistry 75 ( 10 ) 1951 - 1956 2011年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Tocopherols are essential micronutrients for mammals widely known as potent lipid-soluble antioxidants that are present in cell membranes. Recent studies have demonstrated that most of the carboxychromanol (CEHC), a tocopherol metabolite, in the plasma exists primarily in sulfate- and glucuronide- conjugated forms. To gain insight into the enzymatic sulfation of tocopherols and their metabolites, a systematic investigation was performed using all 14 known human cytosolic sulfotransferases (SULTs). The results showed that the members of the SULT1 family displayed stronger sulfating activities toward tocopherols and their metabolites. These enzymes showed a substrate preference for γ-tocopherol over α-tocopherol and for γ-CEHC over other CEHCs. Using A549 human lung epithelial cells in a metabolic labeling study, a similar trend in the sulfation of tocopherols and CEHCs was observed. Collectively, the results obtained indicate that SULTmediated enzymatic sulfation of tocopherols and their metabolites is a significant pathway for regulation of the homeostasis and physiological functions of these important compounds.
DOI： 10.1271/bbb.110352
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プロテオーム解析による食品機能の研究(<特集>食の機能・安全を科学する-おいしさから生理活性,安全性の評価まで)
榊原 陽一, 中原 幸太, 森永 浩通, 山崎 正夫, 西山 和夫, 水光 正仁
生物工学会誌 : seibutsu-kogaku kaishi 89 ( 10 ) 593 - 596 2011年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人日本生物工学会
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食品成分の網羅的な機能性評価法(<特集>食の機能・安全を科学する-おいしさから生理活性,安全性の評価まで)
江藤 望, 山森 一人, 永濱 清子, 榊原 陽一, 西山 和夫, 吉原 郁夫, 水光 正仁
生物工学会誌 : seibutsu-kogaku kaishi 89 ( 10 ) 589 - 592 2011年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人日本生物工学会
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Efficient Approach for Simultaneous Estimation of the Multiple Health-Promoting Effects of Foods 査読あり
Nagahama, Kiyoko; Eto, Nozomu; Yamamori, Kunihito; Nishiyama, Kazuo; Sakakibara, Yoichi; Iwata, Takako; Uchida, Asuka; Yoshihara, Ikuo; Suiko, Masahito
Journal of Agricultural and Food Chemistry 59 ( 16 ) 8575 - 8588 2011年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Efficient approach for simultaneous estimation of multiple health-promoting effects of foods 査読あり
Nagahama K., Eto N., Eto N., Yamamori K., Nishiyama K., Sakakibara Y., Sakakibara Y., Iwata T., Iwata T., Uchida A., Uchida A., Yoshihara I., Suiko M., Suiko M.
Journal of Agricultural and Food Chemistry 59 ( 16 ) 8575 - 8588 2011年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Agricultural and Food Chemistry
The investigation of new food constituents for purposes of disease prevention or health promotion is an area of increasing interest in food science. This paper proposes a new system that allows for simultaneous estimation of the multiple health-promoting effects of food constituents using informatics. The model utilizes expression data of intracellular marker proteins as descriptors that reply to stimulation of a constituent. To estimate three health-promoting effects, namely, cancer cell growth suppression activity, antiviral activity, and antioxidant stress activity, each model was constructed using expression data of marker proteins as input data and health-promoting effects as the output value. When prediction performances of three types of mathematical models constructed by simple, multiple regressions, or artificial neural network (ANN), were compared, the most adequate model was the one constructed using an ANN. There were no statistically significant differences between the actual data and estimated values calculated by the ANN models. This system was able to simultaneously estimate health-promoting effects with reasonable precision from the same expression data of marker proteins. This novel system should prove to be an interesting platform for evaluation of the health-promoting effects of food. © 2011 American Chemical Society.
DOI： 10.1021/jf201836g
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Enzymatic Sulfation of Tocopherols and Tocopherol Metabolites by Human Cytosolic Sulfotransferases. 査読あり
Hashiguchi, T., Kurogi, K., Sakakibara, Y.ほか
Bioscience, Biotechnology, and Biochemistry 75 ( 10 ) 1951 - 1956 2011年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Identification of self-incompatibility related proteins in the pistil of Japanese pear (Pyrus pyrifolia (Burm.f.)) by proteome analysis 査読あり
Uchida, A., Sassa, H., Takenaka, S., Sakakibara, Y., Suiko, M., Kunitake, H.
Plant Omics J. 2011年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Inhibition of proliferation by agricultural plant extracts in seven human adult T-cell leukaemia (ATL)-related cell lines. 査読あり
Kai H, Akamatsu E, Torii E, Kodama H, Yukizaki C, Sakakibara Y, Suiko M, Morishita K, Kataoka H, Matsuno K.
J. Nat. Med. 65 ( 3-4 ) 651 - 655 2011年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sulfation of chlorotyrosine and nitrotyrosine by human lung endothelial and epithelial cells: Role of the human SULT1A3. 査読あり
Yasuda S, Yasuda T, Liu MY, Shetty S, Idell S, Boggaram V, Suiko M, Sakakibara Y, Fu J, Liu MC.
Toxicol. Appl. Pharmacol. 251 ( 2 ) 104 - 109 2011年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yasuda S., Yasuda S., Yasuda T., Liu M., Shetty S., Idell S., Boggaram V., Suiko M., Sakakibara Y., Fu J., Liu M.
Toxicology and Applied Pharmacology 251 ( 2 ) 104 - 109 2011年3月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Toxicology and Applied Pharmacology
During inflammation, potent reactive oxidants formed may cause chlorination and nitration of both free and protein-bound tyrosine. In addition to serving as biomarkers of inflammation-mediated oxidative stress, elevated levels of chlorotyrosine and nitrotyrosine have been linked to the pathogenesis of lung and vascular disorders. The current study was designed to investigate whether the lung cells are equipped with mechanisms for counteracting these tyrosine derivatives. By metabolic labeling, chlorotyrosine O-[35S]sulfate and nitrotyrosine O-[35S]sulfate were found to be generated and released into the labeling media of human lung endothelial and epithelial cells labeled with [35S]sulfate in the presence of added chlorotyrosine and nitrotyrosine. Enzymatic assays using the eleven known human cytosolic sulfotransferases (SULTs) revealed SULT1A3 as the enzyme responsible for catalyzing the sulfation of chlorotyrosine and nitrotyrosine. Reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated the expression of SULT1A3 in the lung endothelial and epithelial cells used in this study. Kinetic constants of the sulfation of chlorotyrosine and nitrotyrosine by SULT1A3 were determined. Collectively, these results suggest that sulfation by SULT1A3 in lung endothelial and epithelial cells may play a role in the inactivation and/or disposal of excess chlorotyrosine and nitrotyrosine generated during inflammation. © 2010 Elsevier Inc.
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Identification, characterization, and ontogenic study of a catechol O-methyltransferase from zebrafish. 査読あり
Alazizi A, Liu MY, Williams FE, Kurogi K, Sakakibara Y, Suiko M, Liu MC.
Aquat. Toxicol. 102 ( 1-2 ) 18 - 23 2011年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Alazizi A., Liu M., Williams F., Kurogi K., Kurogi K., Sakakibara Y., Suiko M., Liu M.
Aquatic Toxicology 102 ( 1-2 ) 18 - 23 2011年3月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Aquatic Toxicology
To establish the zebrafish as a model for investigating the methylation pathway of drug metabolism, we embarked on the molecular cloning of the zebrafish catechol O-methyltransferase (COMT). By searching the GenBank database, a zebrafish nucleotide sequence encoding a putative COMT was identified. Based on the sequence information, we designed and synthesized oligonucleotides corresponding to its 5'- and 3'-coding regions of this zebrafish COMT. Using the first-strand cDNA reverse-transcribed from the total RNA isolated from a 3-month-old adult female zebrafish as the template, the cDNA encoding the zebrafish COMT was PCR-amplified. The recombinant zebrafish COMT protein was subsequently expressed in and purified from BL21 (DE3) Escherichia coli cells transformed with the pGEX-2TK expression vector harboring the zebrafish COMT cDNA. Upon enzymatic characterization, purified COMT displayed methylating activity toward dopamine, dopa, and catecholestrogens, as well as three representative catechol drugs, methyldopa, dobutamine, and isoproterenol. A reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed developmental stage-dependent expression of the zebrafish COMT during embryonic development and throughout the larval stage onto maturity. These results provide a foundation for investigating the involvement of COMT-mediated methylation in protection against the adverse effects of catechol drugs and other xenobiotic catechols during the developmental process. © 2010 Elsevier B.V.
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中国餅麹(曲)由来の新規黒色系糸状菌の焼酎醸造特性 査読あり
野崎 直樹ほか6名
生物工学会誌 89 ( 1 ) 20 - 26 2011年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Novel screening method for potential skin-whitening compounds by a luciferase reporter assay. 査読あり
Shirasugi I, Sakakibara Y, Yamasaki M, Nishiyama K, Matsui T, Liu MC, Suiko M.
Biosci. Biotechnol. Biochem. 74 ( 11 ) 2253 - 2258 2010年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Genistein induced apoptotic cell death in adult t-cell leukemia cells through estrogen receptors 査読あり
Yamasaki M., Mukai A., Ohba M., Mine Y., Sakakibara Y., Suiko M., Morishita K., Nishiyama K.
Bioscience, Biotechnology and Biochemistry 74 ( 10 ) 2113 - 2115 2010年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Adult T-cell leukemia (ATL) occurs in human T-lymphotropic virus type I-infected individuals and is endemic to the southwestern area of Kyushu in Japan. Here, we found that nM levels of genistein and 17-estradiol had cytotoxic effects on ATL cells and activated caspase-3. The estrogen receptor antagonist ICI182780 negated the cytotoxic effect of genistein. In addition, G protein-coupled estrogen receptor agonist G-1 also had a cytotoxic effect on ATL cells. This is the first report suggesting that estrogen receptors are a molecular target for ATL therapy.
DOI： 10.1271/bbb.100359
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Genistein induced apoptotic cell death in adult T-cell leukemia cells through estrogen receptors. 査読あり
Yamasaki M, Mukai A, Ohba M, Mine Y, Sakakibara Y, Suiko M, Morishita K, Nishiyama K.
Biosci. Biotechnol. Biochem. 74 ( 10 ) 2113 - 2115 2010年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Hui Y., Hui Y., Yasuda T., Yasuda S., Liu M., Sakakibara Y., Suiko M., Wall K., Liu M.
Biological and Pharmaceutical Bulletin 33 ( 9 ) 1633 - 1637 2010年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biological and Pharmaceutical Bulletin
Prolonged exposure to high level of estrogen is a known risk factor for breast carcinogenesis. It has been suggested recently that nitrative stress may be an etiologic factor for breast carcinogenesis. Since sulfation plays a major role in the homeostasis of estrogens and their metabolites, we attempted in the present study to find out whether nitrative stress may affect the homeostasis of estrogens through sulfation. Metabolic labeling experiments revealed that the amount of sulfated 17β-estradiol or 4-methoxyestradiol decreased dramatically in MCF- 10A mammary epithelial cells incubated in the presence of 3-morpholinosydnonimine (SIN-1) or diethylenetriamine NONOate (DETA NONOate), two nitric oxide donors commonly used to simulate nitrative stress conditions. In searching for the mechanism underlying the decrease of the sulfation of 17β-estradiol and 4- methoxyestradiol, we demonstrated in an in vitro nitration experiment, that the human cytosolic sulfotransferase isoform 1E1 (SULT1E1), a major estrogen-sulfating enzyme, lost its estrogen-sulfating activity proportionately to the degree of nitration on tyrosine residues. Moreover, cell lysates prepared from MCF-10A cells treated with SIN-1 or DETA NONOate also showed much lower 4-methoxyestradiol-sulfating activities, compared with those determined with cell lysate prepared from control MCF-10A cells. © 2010 Pharmaceutical Society of Japan.
DOI： 10.1248/bpb.33.1633
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Inhibitory effects of nitrative stress on the sulfation of 17beta-estradiol and 4-methoxyestradiol by human MCF 10A mammary epithelial cells. 査読あり
Hui Y, Yasuda T, Yasuda S, Liu MY, Sakakibara Y, Suiko M, Wall KA, Liu MC.
Biol. Pharm. Bull. 33 ( 9 ) 1633 - 1637 2010年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Kurogi K., Sakakibara Y., Kamemoto Y., Takahashi S., Yasuda S., Liu M., Suiko M.
FEBS Journal 277 ( 18 ) 3804 - 3811 2010年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：FEBS Journal
Cytosolic sulfotransferase (SULT) SULT2B1b had previously been characterized as a cholesterol sulfotransferase. Like human SULT2B1, mouse SULT2B1b contains a unique, 31 amino acid C-terminal sequence with a proline/serine-rich region, which is not found in members of other SULT families. To gain insight into the functional relevance of this proline/serine-rich region, we constructed a truncated mouse SULT2B1b lacking the 31 C-terminal amino acids, and compared it with the wild-type enzyme. Enzymatic characterization indicated that the catalytic activity was not significantly affected by the absence of those C-terminal residues. Glutathione S-transferase pulldown assays showed that several proteins interacted with mouse SULT2B1b specifically through this C-terminal proline/serine-rich region. Peptide mass fingerprinting revealed that of the five SULT2B1b-binding proteins analyzed, three were cytoskeletal proteins and two were cytoskeleton-binding molecular chaperones. Furthermore, wild-type mouse SULT2B1b, but not the truncated enzyme, was associated with the cytoskeleton in experiments with a cytoskeleton-stabilizing buffer. Collectively, these results suggested that the unique, extended proline/serine-rich C-terminus of mouse SULT2B1b is important for its interaction with cytoskeletal proteins. Such an interaction may allow the enzyme to move along microfilaments such as actin filaments, and catalyze the sulfation of hydroxysteroids, such as cholesterol and pregnenolone, at specific intracellular locations. Structured digital abstract : Sult2B1b (uniprotkb:) physically interacts () with Myosin-Ic (uniprotkb:), Alpha-actinin-1 (uniprotkb:), Alpha-actinin-4 (uniprotkb:), HSP 90-beta (uniprotkb:), Hsc70, (uniprotkb:), Beta-actin (uniprotkb:) and Gamma-actin (uniprotkb:) by pull down. © 2010 FEBS.
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Mouse cytosolic sulfotransferase SULT2B1b interacts with cytoskeletal proteins via a proline/serine-rich C-terminus. 査読あり
Kurogi K, Sakakibara Y, Kamemoto Y, Takahashi S, Yasuda S, Liu MC, Suiko M.
FEBS J. 277 ( 18 ) 3804 - 3811 2010年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Zebrafish as a Model for the Study of the Phase II Cytosolic Sulfotransferases. 招待あり 査読あり
Liu TA, Bhuiyan S, Liu MY, Sugahara T, Sakakibara Y, Suiko M, Yasuda S, Kakuta Y, Kimura M, Williams FE, Liu MC.
Curr Drug Metab. 11 ( 6 ) 538 - 546 2010年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Identification of A Novel Biomarker for Oxidative Stress Induced by Hydrogen Peroxide in Primary Human Hepatocytes Using The 2-nitrobenzenesulfenyl Chloride Isotope Labeling Method. 査読あり
Takami, Y., Uto, H., Tamai, T., Sato, Y., Ishida, Y., Morinaga, H., Sakakibara, Y., Moriuchi, A., Oketani, M., Ido, A., Nakajima, T., Okanoue, T., Tsubouchi, H.
Hepatol. Res. 40 438 - 445 2010年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sulfation of drug compounds by the zebrafish cytosolic sulfotransferases (SULTs). 査読あり
Kurogi, K., Dillon, J., Nasser, A., Liu, M.-Y., Williams, F.E., Sakakibara, Y., Suiko, M., Liu, M.-C.
Drug Metab. Lett. 4 62 - 68 2010年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Shirasugi I., Shirasugi I., Kamada M., Matsui T., Sakakibara Y., Sakakibara Y., Liu M., Suiko M., Suiko M.
Bioscience, Biotechnology and Biochemistry 74 ( 3 ) 579 - 582 2010年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Sulforaphane is a compound widely present in consumed vegetables, particularly broccoli. Previous studies have demonstrated that sulforaphane has many physiological effects including anti-cancer, anti-oxidation, and detoxification. In this study, we found that sulforaphane inhibited melanogenesis and tyrosinase expression. The inhibitory effect of 5μM sulforaphane on melanogenesis was determined to be equivalent to that of 100μM arbutin. In addition, sulforaphane induced phosphorylated extracellular signal-regulated kinase (ERK) and inhibited phosphorylated p38. It has been reported that the phosphorylated mitogenactivated protein (MAP) kinase family (ERK and p38) controls tyrosinase expression. Our data indicate that sulforaphane inhibited melanogenesis and tyrosinase expression by affecting the phosphorylated MAP kinase family. These findings indicate that sulforaphane might be an effective skin-whitening agent.
DOI： 10.1271/bbb.90778
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Sulforaphane Inhibited Melanin Synthesis by Regulating Tyrosinase Gene Expression in B16 Mouse Melanoma Cells. 査読あり
Shirasugi, I., Kamada, M., Matsui, T., Sakakibara, Y., Liu, M.-C., Suiko, M.
Biosci. Biotechnol. Biochem. 74 579 - 582 2010年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Molecular Cloning and Characterization of a Novel Canine Sulfotransferase
Kurogi, K., Sakakibara, Y., Yasuda, S., Liu, M.-C., Suiko, M.
Animal Cell Technology: Basic & Applied Aspects 16 221 - 229 2010年1月
記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス）
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Sulfation of drug compounds by the zebrafish cytosolic sulfotransferases (SULTS) 査読あり
Kurogi K., Kurogi K., Dillon J., Nasser A., Liu M., Williams F., Sakakibara Y., Suiko M., Liu M.
Drug Metabolism Letters 4 ( 2 ) 62 - 68 2010年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Drug Metabolism Letters
To establish the zebrafish as a model to investigate drug metabolism through sulfation, we had previous cloned, expressed, and purified fourteen distinct zebrafish cytosolic sulfotransferases (SULTs). In the present study, we carried a systematic analysis of the sulfating activities of these fourteen zebrafish SULTs toward a panel of drug compounds. Results showed that four of the fourteen zebrafish SULTs showed no detectable activities toward any of the tested drugs. Among the other ten zebrafish SULTs, three SULT1 enzymes (SULT1 ST1, SULT1 ST2, and SULT1 ST3) displayed considerably stronger activities than the others toward the majority of the drug compounds tested. Specifically, SULT1 ST1, SULT1 ST2, and SULT1 ST3 showed the highest specific activities, at 26.9, 29.3, and 31.5 nmol/min/mg, toward aesculetin, 4-methylembelliferone, and dobutamine, respectively. To further investigate the sulfation of tested drugs by the responsible zebrafish SULT enzymes, the kinetics of the sulfation reactions were analyzed. Kinetic constants determined indicated that the sulfation of these drugs by the SULT enzymes tested is likely to be physiologically relevant. A metabolic labeling experiment using cultured zebrafish liver cells and HepG2 human hepatoma cells was performed. Results showed that zebrafish liver cells displayed a similar pattern of sulfation of the drugs tested as that of HepG2 cells, implying that human and zebrafish liver cells may share considerable similarities with regard to their constituent drugsulfating SULT enzymes. © 2010 Bentham Science Publishers Ltd.
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A novel hydroxysteroid-sulfating cytosolic sulfotransferase, SULT3 ST3, from zebrafish: Identification, characterization, and ontogenic study. 査読あり
Yasuda, S., Burgess, M., Yasuda, T., Liu, M.-Y., Bhuiyan, S., Williams, F.E., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
Drug Metab. Lett. 3 217 - 227 2009年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yasuda S., Burgess M., Yasuda T., Liu M., Bhuiyan S., Williams F., Kurogi K., Sakakibara Y., Suiko M., Liu M., Liu M.
Drug Metabolism Letters 3 ( 4 ) 217 - 227 2009年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Drug Metabolism Letters
To establish the zebrafish as a model for investigating the drug metabolism through sulfation, we had embarked on establishing a complete repertoire of the zebrafish Phase II cytosolic sulfotransferases (SULTs). By searching the expressed sequence tag database, a zebrafish cDNA encoding a putative cytosolic sulfotransferase (SULT) was identified. Based on the sequence analysis, this zebrafish SULT was found to belong to the SULT3 gene family. The recombinant protein of the zebrafish SULT, designated SULT3 ST3, was expressed in and purified from BL21 (DE3) Escherichia coli cells transformed with the pGEX-2TK expression vector harboring SULT3 ST3 cDNA. Upon enzymatic characterization, purified SULT3 ST3 displayed sulfating activity toward hydroxysteroids, particularly pregnenolone and dehydroepiandrosterone (DHEA), as well as several drugs among various endogenous and xenobiotic compounds tested as substrates. The pH-dependence and kinetic constants of this enzyme with DHEA were determined. The regulatory effects of various divalent metal cations on the DHEA-sulfating activity of SULT3 ST3 were quantitatively evaluated. A reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed developmental stage-dependent expression of SULT3 ST3 during embryonic development and throughout the larval stage onto maturity. Collectively, these results suggest a possible involvement of the newly discovered SULT3 ST3 in the metabolism of hydroxysteroids and xenobiotics including drugs in zebrafish. © 2009 Bentham Science Publishers Ltd.
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Yamasaki M., Omi Y., Fujii N., Ozaki A., Nakama A., Sakakibara Y., Suiko M., Nishiyama K.
Bioscience, Biotechnology and Biochemistry 73 ( 10 ) 2217 - 2221 2009年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Cruciferous vegetables and their isothiocyanates are promising foods and agents for cancer prevention. We focus here on the effects of mustard oil (SMO) in a variety of the Japanese radish, Shibori Daikon (Raphanus sativus), on the proliferation of 3Y1 rat fibroblasts and the H-ras-transformed derivative, HR-3Y1-2. SMO (1.6 μ, g/ml) inhibited the proliferation of HR-3Y1-2, but not 3Y1 after 24h after treatment. A cell cycle analysis showed that SMO induced G2/M arrest after 6 h, although this effect was not observed 24 h after the treatment. SMO transiently decreased the cellular reduced glutathione level accompanied with up-regulation of the intracellular reactive oxygen species 2-3 h post-treatment. Glutathione ethyl ester and N- acetyl-L-cysteine prevented the growth inhibitory effect of SMO. This mustard oil extract consisted of 95.6% 4-methylthio-3-butenyl isothiocyanate and 4.4% 4-methylthiobutyl isothiocyanate. SMO selectively inhibited H-ras-transformed 3Y1 cells associated with transient oxidative stress via reduced glutathione (GSH) depletion.
DOI： 10.1271/bbb.90322
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Mustard Oil in "Shibori Daikon" a Variety of Japanese Radish, Selectively Inhibits the Proliferation of H-ras-Transformed 3Y1 Cells. 査読あり
Yamasaki, M., Omi, Y., Fujii, N., Ozaki, A., Nakama, A., Sakakibara, Y., Suiko, M., Nishiyama, K.
Biosci. Biotechnol. Biochem. 73 2217 - 2221 2009年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Molecular Cloning, Expression and Characterization of A Novel Mouse SULT6 Cytosolic Sulfotransferase 査読あり
Takahashi Saki, Sakakibara Yoichi, Mishiro Emi, KOURIKI Haruna, NOBE Rika, KUROGI Katsuhisa, YASUDA Shin, LIU Ming-Cheh, SUIKO Masahito
The journal of biochemistry 146 ( 3 ) 399 - 405 2009年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japanese Biochemical Society
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On the similar spatial arrangement of active site residues in PAPS-dependent and phenolic sulfate-utilizing sulfotransferases. 査読あり
Teramoto, T., Adachi, R., Sakakibara, Y., Liu, M.-C., Suiko, M., Kimura, M.,Kakuta, Y.
FEBS Lett. 583 3091 - 3094 2009年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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A target-specific approach for the identification of tyrosine-sulfated hemostatic proteins 査読あり
Liu T., Liu T., Yasuda S., Williams F., Liu M., Suiko M., Sakakibara Y., Yang Y., Liu M.
Analytical Biochemistry 390 ( 1 ) 88 - 90 2009年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Analytical Biochemistry
A simple methodology for the identification of hemostatic proteins that are subjected to posttranslational tyrosine sulfation was developed. The procedure involves sequence analysis of members of the three hemostatic pathways using the Sulfinator prediction algorithm, followed by [35S]sulfate labeling of cultured HepG2 human hepatoma cells, immunoprecipitation of targeted [35S]sulfate-labeled hemostatic proteins, and tyrosine O-[35S]sulfate analysis of immunoprecipitated proteins. Three new tyrosine-sulfated hemostatic proteins-protein S, prekallikrein, and plasminogen-were identified. Such a target-specific approach will allow investigation of tyrosine-sulfated proteins of other biochemical/physiological pathways/processes and contribute to a better understanding of the functional role of posttranslational tyrosine sulfation. © 2009 Elsevier Inc. All rights reserved.
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Concerted action of the cytosolic sulfotransferase, SULT1A3, and catechol-O-methyltransferase in the metabolism of dopamine in SK-N-MC human neuroblastoma cells
Shin Yasudaa, 1, Tomoko Yasudaa, Ying Huia, b, Ming-Yih Liuc, Masahito Suikod, Yoichi Sakakibarad and Ming-Cheh Liu
Neuroscience Research 64 ( 3 ) 273 - 279 2009年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Molecular Cloning, Expression, and Characterization of A Novel Mouse SULT6 Cytosolic Sulfotransferase 査読あり
Saki Takahashi, Yoichi Sakakibara, Emi Mishiro, Haruna Kouriki, Rika Nobe, Katsuhisa Kurogi, Shin Yasuda, Ming-Cheh Liu, Masahito Suiko
Journal of Biochemistry 2009年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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A target-specific approach for the identification of tyrosine-sulfated hemostatic protein 査読あり
Liu TA, Yasuda S, Williams FE, Liu MY, Suiko M, Sakakibara Y, Yang YS, Liu MC.
Anal Biochem. 2009年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Snapshot of a Michaelis complex in a sulfuryl transfer reaction: Crystal structure of a mouse sulfotransferase, mSULT1D1, complexed with donor substrate and accepter substrate. 査読あり
Teramoto T, Sakakibara Y, Liu MC, Suiko M, Kimura M, Kakuta Y.
Biochem Biophys Res Commun. 2009年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Structural basis for the broad range substrate specificity of a novel mouse cytosolic sulfotransferase--mSULT1D1. 査読あり
Teramoto T, Sakakibara Y, Liu MC, Suiko M, Kimura M, Kakuta Y.
Biochem Biophys Res Commun. 379 ( 1 ) 76 - 80 2009年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Dihydro-alpha-lipoic acid has more potent cytotoxicity than alpha-lipoic acid. 査読あり
amasaki M, Kawabe A, Nishimoto K, Madhyastha H, Sakakibara Y, Suiko M, Okamoto T, Suda T, Uehira K, Nishiyama K.
n Vitro Cell Dev Biol Anim. 2009年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Dihydro-alpha-lipoic acid has more potent cytotoxicity than alpha-lipoic acid 査読あり
Yamasaki M., Kawabe A., Nishimoto K., Madhyastha H., Sakakibara Y., Suiko M., Okamoto T., Suda T., Uehira K., Nishiyama K.
In Vitro Cellular and Developmental Biology - Animal 45 ( 5-6 ) 275 - 280 2009年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：In Vitro Cellular and Developmental Biology - Animal
Alpha-lipoic acid has been shown to possess cancer-cell-killing activity via activation of the apoptosis pathway. In this study, the cytotoxic activities of alpha-lipoic and dihydro-alpha-lipoic acid were compared in HL-60 cells. The cell-killing activity of dihydro-alpha-lipoic acid was higher than that of alpha-lipoic acid. Both alpha-lipoic and dihydro-alpha-lipoic acid induced caspase-3 cleavage and internucleosomal DNA fragmentation in treated cells. On the other hand, apparent necrotic or late-stage apoptotic cell populations could be detected in dihydro-alpha-lipoic acid cells but not in those treated with alpha-lipoic acid. Moreover, dihydro-alpha-lipoic acid, but not alpha-lipoic acid, induced marked mitochondrial permeability transition. Antioxidants could not prevent dihydro-alpha-lipoic- or alpha-lipoic-acidinduced cell death. In addition, dihydro-alpha-lipoic and alpha-lipoic acid did not up-regulate cellular reactive oxygen level. These results indicated that dihydro-alpha-lipoic acid exerts more potent cytotoxicity than alpha-lipoic acid through different cytotoxic actions. © The Society for In Vitro Biology 2008.
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Crystal sturucture of mSULT1D1, a mouse catecholamine sulfotransferase. 査読あり
Teramoto, T., Sakakibara, Y., Inaba, K., Kurogi, K., Liu, M.-C., Suiko, M., Kimura, M., Kakuta, Y.
FEBS Letter 582 3909 - 3914 2008年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Molecular cloning, expression, and characterization of mouse amine N-sulfotransferases. 査読あり
Takahashi S, Sakakibara Y, Mishiro E, Kouriki H, Nobe R, Kurogi K, Yasuda S, Liu MC, Suiko M.
Biochemical Biophysical Research Communication 375 ( 4 ) 531 - 535 2008年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Molecular cloning, expression, and characterization of mouse amine N-sulfotransferases 査読あり
Takahashi S., Takahashi S., Sakakibara Y., Sakakibara Y., Mishiro E., Mishiro E., Kouriki H., Nobe R., Nobe R., Kurogi K., Yasuda S., Yasuda S., Liu M., Suiko M., Suiko M.
Biochemical and Biophysical Research Communications 375 ( 4 ) 531 - 535 2008年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochemical and Biophysical Research Communications
By searching the GenBank database, we recently identified a novel mouse cytosolic sulfotransferase (SULT) cDNA (IMAGE Clone ID 679629) and a novel mouse SULT gene (LOC 215895). Sequence analysis revealed that both mouse SULTs belong to the cytosolic SULT3 gene family. The recombinant form of these two newly identified SULTs, designated SULT3A1 and SULT3A2, were expressed using the pGEX-4T-1 glutathione S-transferase fusion system and purified from transformed BL21 Escherichia coli cells. Both purified SULT3A1 and SULT3A2 exhibited strong amine N-sulfonating activities toward 1-naphthylamine among a variety of endogenous and xenobiotic compounds tested as substrates. Kinetic constants of the sulfation of 1-naphthylamine and 1-naphthol by these two enzymes were determined. Collectively, these results imply that these two amine-sulfonating SULT3s may play essential roles in the metabolism and detoxification of aromatic amine compounds in the body. © 2008 Elsevier Inc.
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Characterization and ontogenic study of novel steroid-sulfating SULT3 sulfotransferases from zebrafish 査読あり
Tomoko Yasuda, Shin Yasuda, Frederick E. Williams, Ming-Yih Liu, Yoichi Sakakibara, Shakhawat Bhuiyan, Rhodora Snow, Glendora Carter, Ming-Cheh Liu
Molecular Cellular Endocrinology 2008年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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抗変異原活性をもつ宮崎県産農産物の探索 査読あり
岩田喬子、榊原陽一、赤松絵奈、酒井美穂、柚木崎千鶴子、山崎正夫、西山和夫、水光正仁
宮崎大学農学部報 54 69 - 76 2007年12月
記述言語：日本語 掲載種別：研究論文（大学，研究機関等紀要）
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Soy-derived isoflavones inhibit the growth of adult T-cell leukemia cells in vitro and in vivo 査読あり
Yamasaki, M., Fujita, S., Ishiyama, E., Mukai, A., Madhyastha, H., Sakakibara, Y., Suiko, M., Hatakeyama, K., Nemoto, T., Morishita, K., Kataoka, H., Tsubouchi, H. and Nishiyama, K.
Cancer Science 98 ( 11 ) 1740 - 1746 2007年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Hydroxylated serotonin and dopamine as substrates and inhibitors for human cytosolic SULT1A3. 査読あり
Yasuda S., Liu M.Y., Suiko M., Sakakibara Y., Liu M.C.
Journal of Neurochemistry 103 ( 6 ) 2679 - 2689 2007年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Differential enzymatic characteristics and tissue-specific expression of human TPST-1 and TPST-2. 査読あり
Mishiro E, Sakakibara Y, Liu MC, Suiko M.
Journal of Biochemistry 140 ( 5 ) 731 - 737 2006年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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リポ酸によるヒト白血病細胞HL-60のアポトーシス誘導効果
山崎正夫、山根理学、西本健太郎、榊原陽一、水光正仁、西山和夫
FOOD FUNCTION 2 ( 1 ) 1 - 5 2006年1月
記述言語：日本語 掲載種別：研究論文（その他学術会議資料等）
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本格焼酎の基本味を識別する脂質膜センサ
中原徳昭、榊原陽一、西山和夫、水光正仁 その他３名
日本食品科学工学会誌 52 ( 8 ) 355 - 365 2005年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Identification of a novel thyroid hormone-sulfating cytosolic sulfotransferase, SULT1 ST5, from zebrafish
Yasuda, S., Kumar, A.P., Liu, M.-Y., Sakakibara, Y. その他３名
FEBS Journal 272 ( 15 ) 3828 - 3837 2005年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Taste evaluation of Honkakushochu with taste sensor (research on the taste of Honkakushochu part I)
Nakahara N., Nakahara N., Sakaida H., Sakaida H., Kai T., Sakakibara Y., Nishiyama K., Fukuda N., Suiko M.
Nippon Shokuhin Kagaku Kogaku Kaishi 52 ( 4 ) 145 - 153 2005年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nippon Shokuhin Kagaku Kogaku Kaishi
The possibility of use of taste sensors equipped with lipid/polymer membranes as one of the techniques which evaluate the taste of Honkakushochu was investigated. First, in order to determine chemical composition of a standard sample, the inorganic component of Honkakushochu was analyzed. The most abundant cation was sodium ion and the richest anion was chloride ion. These ions are considered to be brought in from the mother water used for dilution of Honkakushochu. Next, the measurement with sufficient reproducibility became possible by preparing the standard sample for the taste sensor suitable for evaluation of Honkakushochu. Measuring 41 kinds of Honkakushochu including rice, barley, sweet potato, buckwheat, brown sugar shochu and awamori using 8 taste sensors enabled to classify them based on their materials. Moreover, visual expression was able to be obtained about the taste patterns. Analyses of the data obtained from the taste sensor using the statistical techniques such as principal component analysis and cluster analysis suggested that the taste sensor could be useful for new product development or process control of Honkakushochu.
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味覚センサを用いた本格焼酎の味評価
中原徳昭、榊原陽一、西山和夫、水光正仁 その他３名
日本食品科学工学会誌 52 ( 4 ) 145 - 153 2005年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Biocides, tributyltin and triphenyltin, as possible inhibitors of the human sulfotransferase involved in the estrogen homeostasis
Ohkimoto, K., Sakakibara, Y., Suiko, M., Yoshikawa, H.その他２名
Pesticide Biochemistry and Physiology 81 132 - 138 2005年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Retronasal Aroma Simulatorを用いた穀類本格焼酎の含み香と立ち香分析
境田博至、榊原陽一、西山和夫、水光正仁 その他３名
日本食品科学工学会誌第52巻1号19-26頁 2005年1月
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Cloning and expression of a novel Trichoderma viride laminarinase AI gene (lamAI)
Nobe, R., Sakakibara, Y., Ogawa, K., Suiko, M.
Bioscience,Biotechnologyand Biochemistry 68 102111 - 102119 2004年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Zebrafish tyrosylprotein sulfotransferase: molecular cloning, expression,and functional characterization
Mishiro, E., Liu, M.-Y., Sakakibara, Y.Suiko, M., Liu, M.-C.
Biochemistry and Cell Biology 82 ( 2 ) 295 - 303 2004年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Characterization of a putative zebrafish estrogen sulfotransferase: inhibitory effects and mechanism of action of phytoestrogens
Ohkimoto, K., Liu, M.-Y., Suiko, M.Sakakibara, Y., Liu, M.-C.
Chemico-Biological InteractionsVol. 147, No. 1, 1-7 2004年1月
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蕎麦焼酎の特徴香及び穀類本格焼酎の揮発性成分分析について
境田博至、榊原陽一、西山和夫、水光正仁 その他５名
日本食品科学工学会誌第50巻12号555-562頁 2003年12月
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Sulfonation of environmental estrogens by zebrafish cytosolic sulfotransferases
Ohkimoto, K., Sugahara, T.,Sakakibara, Y., Suiko, M.その他３名
Biochemical and Biophysiccal ResearchCommunications Vol. 309, No. 17-11 2003年9月
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Purification and characterization of laminaran hydrolases from Trichoderma viride
Nobe, R., Sakakibara, Y., Fukuda, N.Yoshida, N., Ogawa, K., Suiko, M.
Bioscience,Biotechnologyand Biochemistry Vol. 67, No. 21349-1357 2003年6月
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Sulfation of hydroxychlorobiphenyls: Molecular cloning, expression, and functional characterization of zebrafish SULT1 sulfotransferases
Sugahara, T., Liu, C.-C., Suiko, M.Sakakibara, Y.その他４名
European Journal of BiochemistryVol. 270, No. 112404-2411 2003年6月
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Differential roles of human monoamine (M)-form and simplephenol (P)-form phenol sulfotransferases in drug metabolism
Sugahara, T., Pai, T.G., Suiko, M.Sakakibara, Y., Liu, M.-C.
Journal of BiochemistryVol. 133. No. 2259-262 2003年2月
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Structure-function relationships in the stereospecific and manganese-dependent dopa/tyrosine-sulfating activity of human monoamine-form phenol sulfotransferase, SULT1A3
Pai, T.G., Sugahara, T., Suiko, M.Sakakibara, Y.その他２名
The Journal ofBiological Chemistry Vol. 278, No.31525-1532 2003年1月
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Manganese stimulation and stereospecificity of the dopa (3,4-dihydroxyphenylalanine)/tyrosine-sulfating activity of human monoamine-form phenol sulfotransferase. kinetic studies of the mechanism using wild-type and mutant enzymes
Pai, T.G., Ohkimoto, K., Sakakibara, Y.Suiko, M.その他２名
The Journal ofBiological Chemistry Vol.277, No.4643813-43820 2002年11月
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Differential xenoestrogen-sulfating activities of the human cytosolic sulfotransferases: molecular cloning, expression, and purification of human SULT2B1a and SULT2B1b sulfotransferases
Pai, T.G., Sugahara, T., Suiko, M.Sakakibara, Y.その他２名
BiochimicaBiophysicaActaVol. 1573, No. 2165-170 2002年11月
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Regulatory effects of divalent metal cations on human cytosolic sulfotransferases
Xu, F., Suiko, M., Sakakibara, Y.Pai, T.G., Liu, M.-C.
Journal of BiochemistryVol.132, No.3457-462 2002年9月
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Highly conserved mouse and human brain sulfotransferases: molecular cloning, expression, and functional characterization
Sakakibara, Y., Suiko, M., Pai, T.G.Nakayama, T.その他３名
GeneVol. 285, No. 1-239-47 2002年2月
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Sulfation of flavonoids and other phenolic dietary compounds by the human cytosolic sulfotransferases
Pai, T.G., Suiko, M., Sakakibara, Y.Liu, M.-C
Biochemical and Biophysiccal ResearchCommunications Vol. 285, No. 31175-1179 2001年8月
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Ranasinghe J., Liu M., Sakakibara Y., Sakakibara Y., Takeshita Y., Fukuda N., Nasu T., Suiko M.
Journal of Molecular Catalysis - B Enzymatic 12 ( 1-6 ) 131 - 136 2001年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Molecular Catalysis - B Enzymatic
In our previous studies, we have demonstrated the stereoselective and manganese-dependent sulfation of tyrosine and Dopa isomers by human monoamine (M)-form phenol sulfotransferase (PST). In the present study, we investigated the occurrence of these phenomena in vivo using Sprague-Dawley rats as an experimental model. Three groups of six male rats were orally introduced with 1ml of, respectively, 3mM, 10mM and 30mM MnCl2 with a constant level of 0.2mM DL-m-tyrosine per day for 7 days. Their urine was collected and analyzed for the presence of sulfated DL-m-tyrosine (DL-m-TyrS) by ion-pair HPLC using a C18 reversed-phase column. The level of urinary DL-m-TyrS, which was detected in the urine of the MnCl2-treated rats but not control rats, appeared to increase proportionally to the amount of MnCl2 administered. Chiral HPLC was employed to differentiate the D-form and L-form m-TyrS present in the urine sample of MnCl2-treated rats. Both D-m-TyrS and L-m-TyrS were detected, with the D-form being present at significantly higher level than the L-form. Copyright © 2001 Elsevier Science B.V.
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Stereoselective and manganese-dependent sulfation and urinary excretion of D-form and L-form meta-tyrosine O-sulfate by Sprague-Dawly rats
Ranasinghe, J.G.S., Liu, M.-C.Sakakibara, Y., Takeshita, Y.その他３名
Journal of Molecular Catalysis B:EnzymaticVol. 12, 131-136 2001年2月
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Manganese administration induces the increased production of dopamine sulfate and depletion of dopamine in Sprague-Dawley rats
Ranasinghe, J.G.S., Liu, M.-C.Sakakibara, Y., Suiko, M.
Journal of BiochemistryVol. 128, No. 3477-480 2000年9月
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Mutational analysis of the substrate-binding/catalytic domains of human M-form and P-form phenol sulfotransferases
Liu, M.-C., Suiko, M., Sakakibara, Y.
The Journal ofBiological ChemistryVol. 275, No. 1813460-13464 2000年5月
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Sulfation of environmental estrogen-like chemicals by human cytosolic sulfotransferases
Suiko, M., Sakakibara, Y., Liu, M.-C.
Biochemical and Biophysiccal ResearchCommunications Vol. 267, No. 180-84 2000年1月
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Maturation activation and protection of dendritic cells induced by double-stranded RNA
Cella, M. , Salio, M., Sakakibara, Y. Langen, H.その他２名
Journal of Experimental MedicineVol. 189, No. 5821-829 1999年3月
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Bacterial expression, purification, and characterization of a novel mouse sulfotransferase that catalyzes the sulfation of eicosanoids
Liu, M.-C., Sakakibara, Y., Liu, C.-C.
Biochemical and Biophysiccal ResearchCommunicationsVol. 254, No. 165-69 1999年1月
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T lymphocyte costimulation mediated byreorganization of membrane microdomains
Viola, A., Schroeder, S., Sakakibara, Y. Lanzavecchia, A.
ScienceVol. 283, No. 5402680-682 1999年1月
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Structural identification of sulfated tyrosine in human urine
Ranasinghe, J.G.S., Sakakibara, Y.Harada, M., Nishiyama, K.その他２名
Bioscience,Biotechnologyand BiochemistryVol. 63, No. 1229-231 1999年1月
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Sakakibara Y., Yanagisawa K., Katafuchi J., Ringer D., Takami Y., Nakayama T., Suiko M., Liu M., Liu M.
Journal of Biological Chemistry 273 ( 51 ) 33929 - 33935 1998年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Biological Chemistry
Upon sulfonation, carcinogenic hydroxyarylamines such as N-hydroxy-2- acetylaminofiuorene (N-OH-2AAF) can be further activated to form ultimate carcinogens in vivo. Previous studies have shown that a SULT1C1 sulfotransferase is primarily responsible for the sulfonation of N-OH-2AAF in rat liver. In the present study, two novel human sulfotransferases shown to be members of the SULT1C sulfotransferase subfamily based on sequence analysis have been cloned, expressed, and characterized. Comparisons of the deduced amino acid sequence encoded by the human SULTIC sulfotransferase cDNA 1 reveal 63.7, 61.6, and 85.1% identity to the amino acid sequences of rat SULT1C1 sulfotransferase, mouse SULTIC1 sulfotransferase, and rabbit SULT1C sulfotransferase. In contrast, the deduced amino acid sequence of the human SULT1C sulfotransferase 2 cDNA displays 62.9, 63.1, 63.1, and 62.5% identity to the amino acid sequences of the human SULT1C sulfotransferase 1, rat SULT1C1 sulfotransferase, mouse SULT1C1 sulfotransferase, and rabbit SULT1C sulfotransferase. Recombinant human SULT1C sulfotransferases 1 and 2, expressed in Escherichia coli and purified to near electrophoretic homogeneity, were shown to cross-react with the antiserum against the rat liver SULT1C1 sulfotransferase and exhibited sulfonating activities with N- OH-2AAF as substrate. Tissue-specific expression of these novel human SULT1C sulfotransferases were examined by employing the Northern blotting technique. The results provide a foundation for the investigation into the functional relevance of these new SULT1C sulfotransferases in different human tissues/organs.
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Molecular cloning, expression, and characterization of novel human SULT1C sulfotransferases that catalyze the sulfonation of N-hydroxy-2-acetylaminofluorene
Sakakibara, Y., Yanagisawa, K.Katafuchi, J., Ringer, D.P.その他４名
The Journal ofBiological ChemistryVol. 273, No. 5133929-33935 1998年12月
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Substrate specificity of human monoamine (M)-form phenol sulfotransferase: Preparation and analysis of dopa 3-O-sulfate and dopa 4-O-sulfate
Suiko, M., Sakakibara, Y.Awan-Khan, R., Sakaida, H.その他３名
Journal ofBiochemistryVol. 124, No. 4707-711 1998年10月
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Molecular cloning, expression, and characterization of a novel mouse liver SULT1B1 sulfotransferase
Saeki, Y., Sakakibara, Y., Araki, Y.Yanagisawa, K.その他３名
Journal ofBiochemistryVol. 124, No. 155-64 1998年7月
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Molecular cloning, expression, and functional characterization of novel mouse sulfotransferases
Sakakibara, Y., Yanagisawa, K.Takami, Y., Nakayama, T.その他２名
Biochemical and Biophysiccal ResearchCommunicationsVol. 247, No. 3681-686 1998年6月
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cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase enzyme
Yanagisawa, K., Sakakibara, Y.Suiko, M., Takami, Y.その他５名
Bioscience, Biotechnologyand BiochemistryVol. 62, No. 51037-1040 1998年5月
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Localization and functional analysis of the substrate specificity/catalytic domains of human M-form and P-form phenol sulfotransferases
Sakakibara, Y., Takami, Y., Nakayama, T.Suiko, M., Liu, M.-C.
The Journal ofBiological ChemistryVol. 273, No. 116242-6247 1998年3月
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Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites
Gowda, K., Black, S., Moeller, I.Sakakibara, Y.その他２名
Gene Vol. 207, No. 2197-207 1998年1月
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Yanagisawa K., Sakakibara Y., Suiko M., Takami Y., Nakayama T., Nakajima H., Takayanagi K., Natori Y., Liu M.
Bioscience, Biotechnology and Biochemistry 62 ( 5 ) 1037 - 1040 1998年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
A cDNA encoding the human bifunctional ATP sulfurylase/adenosine 5′-phosphosulfate (APS) kinase was cloned and sequenced. The enzyme contains an APS kinase domain in its N-terminal portion and an ATP sulfurylase domain in its C-terminal portion. Recombinant full-length enzyme and its constituent APS kinase and ATP sulfurylase domains were individually expressed, purified, and shown to have their respective enzymatic activities. © 1998, Taylor & Francis Group, LLC. All rights reserved.
DOI： 10.1271/bbb.62.1037
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Characterization of bovine heart sulfotransferase catalyzing the sulfation of tyrosine-containing peptides
Suiko, M., Fern o, P.H.P.Sakakibara, Y., Kudo, H.その他２名
Journal of Nutritional Science andVitaminologyVol. 43, No. 3485-490 1997年8月
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Tissue-specific and developmental stage-dependent expression of a novel rat dopa/tyrosine sulfotransferase
Araki, Y., Sakakibara, Y., Boggaram, B.Katafuchi, J.その他３名
International Journal of Biochemistry andCell BiologyVol. 29, No. 5801-806 1997年5月
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Biochemistry of the sulfation of dopa and tyrosine isomers: investigation of a novel dopa/tyrosine sulfotransferase
Sakakibara, Y., Suiko, M., Nishiyama, K.Nakajima, H., Liu, M.-C.
Animal CellTechnology:Basic & Applied AspectsVol. 8309-314 1997年4月
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Role of a putative tyrosine-O-sulfate receptor in the targeting and/or intracellular transport of tyrosine-sulfated proteins
Liu, M.-C., Sakakibara, Y., Suiko, M.
CytotechnologyVol. 23, No. 1-3143-149 1997年3月
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Sakakibara Y., Katafuchi J., Katafuchi J., Takami Y., Nakayama T., Suiko M., Suiko M., Nakajima H., Liu M.
Biochimica et Biophysica Acta - Molecular Cell Research 1355 ( 2 ) 102 - 106 1997年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochimica et Biophysica Acta - Molecular Cell Research
Human monoamine (M)-form phenol sulfotransferase (PST) was PCR-cloned and transiently expressed in COS-7 cells. The recombinant enzyme was demonstrated to display not only the previously reported sulfotransferase activity toward dopamine, but also novel manganese-dependent Dopa/tyrosine sulfotransferase activities. These results imply a new functional role of the human M-form PST in the homeostatic regulation of Dopa and tyrosine.
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Manganese-dependent dopa/tyrosine sulfation in HepG2 human hepatoma cells: novel dopa/tyrosine sulfotransferase activities associated with the human monoamine-form phenol sulfotransferase
Sakakibara, Y., Katafuchi, J.Takami, Y., Nakayama, T.その他２名
BiochimicaBiophysicaActaVol. 1355, No. 2102-106 1997年2月
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Suiko Masahito, Fernando P.H.Prasantha, Sakakibara Yoichi, KUDO Hisao, NAKAMURA Toyohiko, LIU Ming-Cheh
Journal of Nutritional Science and Vitaminology 43 ( 4 ) 485 - 490 1997年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本ビタミン学会
Using [35S]PAPS as the sulfate donor, we have detected a sulfotransferase from bovine heart which catalyzes the sulfation of tyrosine-containing peptides. The enzyme displayed optimal activity at pH 5.75 and 35°C in a one-hour reaction. The addition of 10 mM Mn2+ or Co2+ to the reaction mixture increased the sulfotransferase activity by 3.4 and 3.5-fold, respectively. In contrast, the maximum increment stimulated by Mg2+ was only 1.75-fold at 15 mM concentration, and instead of exerting an enhancement effect, Ca2+ was found to be a potent inhibitor. The addition of 50 mM NaF to the reaction mixture resulted in an increase in sulfotransferase activity of 3.3-fold. The Km for 3'-phosphoadenosine 5'-phosphosulfate (PAPS) was determined to be 2μM at a constant 0.5 mM Boc-Glu-Asp-Tyr-Val. Among the 10 peptides tested as substrates, Boc-Glu-Asp-Tyr-Val and Boc-Asp-Asp-Tyr-Val provided the highest activities.
DOI： 10.3177/jnsv.43.485
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Suiko M., Suiko M., Sakakibara Y., Sakakibara Y., Nakajima H., Sakaida H., Sakaida H., Liu M.
Biochemical Journal 314 ( 1 ) 151 - 158 1996年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochemical Journal
HepG2 human hepatoma cells, labelled with [35S]sulphate in media containing L-3,4-dihydroxyphenylalanine (L-dopa), (D-dopa), DL-m-tyrosine or D-p-tyrosine, were found to produce the [35S]sulphated forms of these compounds. Addition to the labelling media of m-hydroxybenzylhydrazine, an aromatic amino acid decarboxylase inhibitor, greatly enhanced the production of L-dopa O-[35S]sulphate and DL-m-tyrosine O-[35S]sulphate, with a concomitant decrease in the formation of dopamine O-[35S]sulphate and m-tyramine O-[35S]sulphate. With 3′-phosphoadenosine 5′-phospho[35S]sulphate as the sulphate donor, HepG2-cell cytosol was shown to contain enzymic activity catalysing the sulphation of L-dopa, D-dopa, L-m-tyrosine, D-m-tyrosine, L-p-tyrosine and D-p-tyrosine. The pH optimum of the enzyme, designated dopa/tyrosine sulphotransferase, was determined to be 8.75 with D-m-tyrosine as the substrate. The enzyme exhibited stereoselectivity for the D-form of dopa or tyrosine isomers. Addition of 10 mM MnCl2 to the reaction mixture resulted in a remarkable stimulation of dopa/tyrosine sulphotransferase activity, being as high as 267.8 times with D-p-tyrosine as the substrate. Quantitative assays revealed L-dopa, D-dopa and D-m-tyrosine to be better substrates than L-p-tyrosine. When the HepG2-cell cytosol was subjected to DEAE Bio-Gel and hydroxyapatite column chromatography, dopa/tyrosine sulphotransferase was co-eluted with the thermolabile 'M-form' phenol sulphotransferase. Furthermore dopa/tyrosine sulphotransferase displayed properties similar to that of the M-form phenol sulphotransferase with respect to thermostability and sensitivity to 2,6-dichloro-4-nitrophenol. Whether the M-form phenol sulphotransferase is truly (solely) responsible for the dopa/tyrosine sulphotransferase activity present in HepG2 cells remains to be clarified.
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Enzymic sulphation of dopa and tyrosine isomers by HepG2 human hepatoma cells: stereoselectivity and stimulationby Mn2+
Suiko, M., Sakakibara, Y., Nakajima, H.Sakaida, H., Liu, M.-C.
Biochemical JournalVol. 314, No. 1151-158 1996年2月
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Desulfation of tyrosine-O-sulfated peptides by some eukaryotic sulfatases
Suiko, M., Tojo, T., Fern o, P.H.P.Sakakibara, Y.その他２名
BioscienceBiotechnologyBiochemistryVol. 60, No. 1137-138 1996年1月
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Purification, characterization, and molecular cloning of a novel rat liver dopa/tyrosine sulfotransferase
Sakakibara, Y., Takami, Y., Zwieb, C.Nakayama, T.その他３名
The Journal of Biological ChemistryVol. 270, No. 5130470-30478 1995年12月
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Sulfation of L-tyrosine in mammalian cells: a comparative study
Sakakibara, Y., Suiko, M., Nakajima, H., Liu, M.-C.
Biochemical JournalVol. 305, No. 3993-998 1995年2月
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De novo sulfation of L-tyrosine in HepG2 human hepatoma cells and its possible functional implication
Sakakibara, Y., Suiko, M., Liu, M.-C.
European Journalof BiochemistryVol. 226, No. 2293-301 1994年12月
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Sulfation of L-tyrosine in higher plants and mammalian animals
Suiko, M., Sakakibara, Y.
Proceeding of IPBARogla, Slovenia211-222 1994年12月
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Purification and characterization of two molecular forms of bovine complement factor H
Sakakibara, Y., Suiko, M.Fern o, P.H.P., Miura, M., Liu, M.-C.
Animal CellTechnology: Basic & Applied AspectsVol. 6581-588 1994年8月
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Sakakibara Y., Sakakibara Y., Suiko M., Fernando P., Ohashi T., Liu M.
Cytotechnology 14 ( 2 ) 97 - 107 1994年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cytotechnology
A major tyrosine-O-sulfate (TyrS)-binding protein present in bovine serum was purified to electrophoretic homogeneity using a combination of TyrS-Affi-Gel 10 affinity chromatographyy, DEAE-Bio-Gel A ion-exchange chromatography, and hydroxylapatite chromatography. The purified TyrS-binding protein migrated as doublet protein bands with apparent molecular weights of ca. 160, 000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. N-termini of the two forms of purified TyrS-binding protein contain most likely identical sequence for the first fifteen amino acids residues, which displays a high degree of homology to those of human and mouse complement factor H. Furthermore, the purified TyrS-binding protein exhibited immunologic cross-reactivity with anti-human complement factor H. These results indicate the identity of the purified TyrS-binding protein being bovine complement factor H. The two forms of the purified bovine factor H were investigated with respect to the sensitivity to limited trypsin digestion. The high-molecular weight form was cleaved into two fragments with apparent molecular masses of, respectively, 45 kD and 125 kD. The low-molecular weight form was cleaved in a different manner to generate three major fragments with molecular masses of 25 kD, 45 kD and 100 kD, respectively. Limited V8 protease mapping of the two forms yielded similar, yet unidentical, peptide band patterns. Purified bovine factor H appeared to bind agarose-bonded heparin through its anion-binding domain and the binding was inhibited by the presence of free heparin or dextran sulfate. © 1994 Kluwer Academic Publishers.
DOI： 10.1007/BF00758174
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Preparation of 3’-phosphoadenosine 5’-phospho35S-sulfate using ATP sulfurylase and APS kinase from Bacillus stearothermophilus: enzymatic synthesis and purification
Fern o, P.H.P., Karakawa, A.Sakakibara, Y., Ibuki, H. その他3名
Bioscience,Biotechnologyand BiochemistryVol. 57, No. 111974-1975 1993年11月
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Isolation and characterization of a novel microsomal membrane-bound phenol sulfotransferase from bovine liver
Fern o, P.H.P., Sakakibara, Y.Nakatsu, S., Suiko, M. その他2名
Biochemistryand MolecularBiologyInternationalVol. 30, No. 3433-441 1993年3月
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Suiko M., Fernando P., Sakakibara Y., Nakajima H., Liu M., Abe S., Nakatsu S.
Nucleic acids symposium series ( 27 ) 183 - 184 1992年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nucleic acids symposium series
In vitro tyrosine sulfation of recombinant proteins would be a valuable tool in converting those proteins expressed in prokaryotic vectors to their natural form. For this purpose tyrosylprotein sulfotransferase (TPST), the enzyme responsible for tyrosine sulfation of proteins, was characterized from a bovine liver Golgi preparation. TPST was active in a acidic environment with a pH optimum of 6.25, and displayed a stimulation by the Mn2+, with the optimum activity in the presence of 5mM MnCl2. TPST was able to sulfate recombinant hirudin variant 1 (rHV-1) expressed in Escherichia coli and the C-terminal hirudin fragment 54-65 but not the N-terminal hirudin fragment 1-15 by using 3'-phosphoadenosine 5'-phosphosulfate (PAPS), indicating its specificity for the naturally sulfated tyrosine 63. Comparison of the reaction kinetics on synthetic peptides showed that the bovine liver TPST has a higher affinity and reaction rates for those peptides with a aspartyl residue on the N-terminal side of the tyrosine when compared with a glutamyl residue.
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Detoxication of phenolic xenobiotics in microsomal membranes: a novel phenol sulfotransferase activity in bovine liver microsomes
Suiko, M., Fern o, P.H.P.Sakakibara, Y., Liu, M.-C.
Proceeding of the6th China-Japan Symposiumon PesticideScience,Fukuoka, Japan151-155 1992年11月
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Protein tyrosine sulfation in animal cells and its simulation in vitro in the downstream processing of proteins
Fern o, P.H.P., Sakakibara, Y.Abe, S., Liu, M.-C. その他２名
Animal Cell Technology: Basic & Applied AspectsVol. 4473-479 1992年8月