Affiliation |
Faculty of Agriculture Department of Animal and Grassland Sciences |
Title |
Associate Professor |
External Link |
ISHIDA Takafumi
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Research Areas 【 display / non-display 】
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Life Science / Animal production science
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Life Science / Animal production science
Papers 【 display / non-display 】
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Metabolic profiles and their correlation with carcass traits in Japanese Black steers under progeny testing Reviewed International coauthorship
Tokunaga T., Jomane F.N., Takahashi T., Katamoto H., Kobayashi I., Yoshimuta K., Okuno R., Ishida T.
Journal of Warm Regional Society of Animal Science, Japan 64 ( 2 ) 89 - 95 2021.9
Authorship:Corresponding author Language:English Publishing type:Research paper (scientific journal) Publisher:Animal science journal = Nihon chikusan Gakkaiho
The objective of this study was to develop mathematical equations for describing the change in marbling in Japanese Black steers using longitudinal measurements. Serial ultrasound measurements were taken at 14, 16, 20, and 26 months of age and analyzed using an image analysis software. The longitudinal marbling measurements from the ultrasound images and carcasses were fitted into a nonlinear logistic curve. Data used for the analysis consisted of 749 steers that converged in nonlinear curve fitting and showed reasonable estimated parameters of the logistic curves. The average predicted mature beef marbling score (BMS) and maturation rate were 6.26 and 0.353, respectively, and the average maturity levels at 24 months of age were 83.9%. The heritability estimates for the predicted maturity traits were moderate, indicating that these traits may have potential for genetic improvement. There was a negative relationship between the expected progeny differences between carcass BMS and maturity traits, suggesting that genetic improvement by carcass BMS may lead to the selection of bulls with late maturity for marbling. The results indicate that ultrasound and model building for marbling can be useful tools to correctly select candidate bulls with high marbling in the early fattening period.
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Estimation of the marbling development pattern in Japanese Black cattle by using serial ultrasound measurement data Reviewed International coauthorship
Tokunaga T., Jomane F.N., Mandai S., Ishida T., Hirooka H.
Animal Science Journal 92 ( 1 ) e13533 2021.1
Language:English Publishing type:Research paper (scientific journal) Publisher:Animal science journal = Nihon chikusan Gakkaiho
The objective of this study was to develop mathematical equations for describing the change in marbling in Japanese Black steers using longitudinal measurements. Serial ultrasound measurements were taken at 14, 16, 20, and 26 months of age and analyzed using an image analysis software. The longitudinal marbling measurements from the ultrasound images and carcasses were fitted into a nonlinear logistic curve. Data used for the analysis consisted of 749 steers that converged in nonlinear curve fitting and showed reasonable estimated parameters of the logistic curves. The average predicted mature beef marbling score (BMS) and maturation rate were 6.26 and 0.353, respectively, and the average maturity levels at 24 months of age were 83.9%. The heritability estimates for the predicted maturity traits were moderate, indicating that these traits may have potential for genetic improvement. There was a negative relationship between the expected progeny differences between carcass BMS and maturity traits, suggesting that genetic improvement by carcass BMS may lead to the selection of bulls with late maturity for marbling. The results indicate that ultrasound and model building for marbling can be useful tools to correctly select candidate bulls with high marbling in the early fattening period.
DOI: 10.1111/asj.13533
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Nakanishi T., Tokunaga T., Ishida T., Kobayashi I., Katahama Y., Yano A., Erickson L., Kawahara S.
Asian-Australasian Journal of Animal Sciences 32 ( 4 ) 592 - 598 2019.4
Language:English Publishing type:Research paper (scientific journal) Publisher:Asian-Australasian Journal of Animal Sciences
Copyright © 2019 by Asian-Australasian Journal of Animal Sciences. Objective: Autophagy is a bulk degradation system for intracellular proteins which contributes to skeletal muscle homeostasis, according to previous studies in humans and rodents. However, there is a lack of information on the physiological role of autophagy in the skeletal muscle of meat animals. This study was planned as a pilot study to investigate changes in expression of two major autophagy-related genes, microtubule-associated protein 1 light chain 3β (MAP1LC3B) and autophagy related 7 (ATG7) in fattening beef cattle, and to compare them with skeletal muscle growth. Methods: Six castrated Japanese Black cattle (initial body weight: 503±20 kg) were enrolled in this study and fattened for 7 months. Three skeletal muscles, M. longissimus, M. gluteus medius, and M. semimembranosus, were collected by needle biopsy three times during the observation period, and mRNA levels of MAP1LC3B and ATG7 were determined by quantitative reverse-transcription polymerase chain reaction. The expression levels of genes associated with the ubiquitin-proteasome system, another proteolytic mechanism, were also analyzed for comparison with autophagy-related genes. In addition, ultrasonic scanning was repeatedly performed to measure M. longissimus area as an index of muscle growth. Results: Our results showed that both MAP1LC3B and ATG7 expression increased over the observation period in all three skeletal muscles. Interestingly, the increase in expression of these two genes in M. longissimus was highly correlated with ultrasonic M. longissimus area and body weight. On the other hand, the expression of genes associated with the ubiquitin-proteasome system was unchanged during the same period. Conclusion: These findings suggest that autophagy plays an important role in the growth of skeletal muscle of fattening beef cattle and imply that autophagic activity affects meat productivity.
DOI: 10.5713/ajas.18.0370
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Polymorphisms of RDH16 and VEGFR1 influence M. trapezius steatosis in Japanese Black carcass Reviewed
Ishida T., Kosuke N., Jomane F., Tokunaga T.
Animal Science Journal 88 ( 8 ) 1037 - 1041 2017.8
Language:English Publishing type:Research paper (scientific journal)
DOI: 10.1111/asj.12746
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Jomane, F.N., Ishida, T., Tokunaga, T., Morita, T.
Animal Science Journal 88 ( 3 ) 413 - 420 2017.3
Language:English Publishing type:Research paper (scientific journal) Publisher:Animal Science Journal
© 2016 Japanese Society of Animal Science Polymorphisms in genes involved in lipid metabolism have been reported to be associated with fatty acid composition of adipose tissue. However, the relationship of these polymorphisms with premortem ultrasonic traits is unknown. The objective of this study, therefore, was to assess the association between polymorphisms in fatty acid synthase, stearoyl-coenzyme A desaturase (SCD), sterol regulatory element-binding protein 1 (SREBP1), diacylglycerol acyltransferase 1, and nuclear receptor subfamily 1, group H, number 3 genes with ultrasonic and carcass traits in Japanese Black steers (n = 300) under progeny testing at the Livestock Improvement Association of Miyazaki. To have a comprehensive analysis of the association between the aforementioned genetic polymorphisms and ultrasonic traits, longitudinal measurements of ultrasonic traits were taken. Furthermore, the association of these genetic polymorphisms and carcass traits was evaluated. The polymorphisms in the SCD gene and SREBP1 were associated (P < 0.05) with some ultrasonic traits at multiple stages. To add to that, the polymorphisms were associated (P < 0.05) with some carcass traits. These findings suggest that the polymorphisms in SCD and SREBP1 are functional mutations or could be related to mutations that can aid in selection to improve some ultrasonic and carcass traits.
DOI: 10.1111/asj.12650
Presentations 【 display / non-display 】
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SNP情報に基づく家畜改良センター繋養黒毛和種雌牛の系統分類
井上慶一、竹田将悠規、一関可純、石田孝史、藤原信一
第60回肉用牛研究会福島大会 (福岡) 2023.10 日本暖地畜産学会
Event date: 2023.10
Language:Japanese Presentation type:Oral presentation (general)
Venue:福岡
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宮崎県における直接検定不合格牛の検定後肥育管理が産肉成績に及ぼす影響
木村 萌・田尻優生・蔭山みなみ・萬代慎太郎・石田孝史・徳永忠昭
第14回日本暖地畜産学会大会 (福岡) 2021.10.23 日本暖地畜産学会
Event date: 2021.10.23 - 2021.10.24
Language:Japanese Presentation type:Oral presentation (general)
Venue:福岡
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黒毛和種肥育牛における肥育中期の皮下脂肪厚が産肉形質に及ぼす影響
蔭山みなみ・日高祐輝・萬代慎太郎・石田孝史・徳永忠昭
第14回日本暖地畜産学会大会 (福岡) 2021.10.23 日本暖地畜産学会
Event date: 2021.10.23 - 2021.10.24
Language:Japanese Presentation type:Oral presentation (general)
Venue:福岡
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免疫学的去勢効果の違いがバークシャー種肥育豚の発育および枝肉に及ぼす影響
寺本真理子・村松蓮太・浦川啓介・石田孝史・徳永忠昭
第14回日本暖地畜産学会大会 (福岡) 2021.10.23 日本暖地畜産学会
Event date: 2021.10.23 - 2021.10.24
Language:Japanese Presentation type:Oral presentation (general)
Venue:福岡
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バークシャー種肥育豚における飼料給与プログラムの違いによる成長への影響
村松蓮太・寺本真理子・有馬義之・石田孝史・徳永忠昭
第14回日本暖地畜産学会大会 (福岡) 2021.10.23 日本暖地畜産学会
Event date: 2021.10.23 - 2021.10.24
Language:Japanese Presentation type:Oral presentation (general)
Venue:福岡
Available Technology 【 display / non-display 】
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黒毛和種の受胎成績に関与する遺伝子型多型に関する研究
黒毛和種枝肉における瑕疵、特にシコリ発生要因の解明に関する研究
黒毛和種の生産関連形質に関与する遺伝子型多型の探索Home Page: 動物遺伝育種学研究室
Related fields where technical consultation is available:・黒毛和種の育種改良
・ウシの遺伝子解析Message:・共同研究の希望テーマ:肉用牛の育種改良のための遺伝子マーカー探索