論文 - 永田 賢治
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[A case of gastric endocrine cell carcinoma with liver metastasis showing rapid growth during pregnancy and marked reduction by systemic chemotherapy after delivery].
Shirado A, Uto H, Kusumoto K, Kurogi J, Sakamoto H, Hasuike S, Nagata K, Hayashi K, Iwamitsu A, Hori T, Ibusuki K, Ido A, Tsubouchi H
Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology 103 ( 7 ) 827 - 32 2006年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Effect of hepatocyte growth factor on endogenous hepatocarcinogenesis in rats fed a choline-deficient L-amino acid-defined diet.
Nakanishi C, Moriuchi A, Ido A, Numata M, Kim ID, Kusumoto K, Hasuike S, Abe H, Nagata K, Akiyama Y, Uto H, Kataoka H, Tsubouchi H
Oncology reports 16 ( 1 ) 25 - 31 2006年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Nakanishi C., Moriuchi A., Ido A., Numata M., Kim I., Kusumoto K., Hasuike S., Abe H., Nagata K., Akiyama Y., Uto H., Kataoka H., Tsubouchi H.
Oncology Reports 16 ( 1 ) 25 - 31 2006年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Oncology Reports
Hepatocyte growth factor (HGF) is a promising agent for the treatment of intractable liver disease, due to its mitogenic, anti-apoptotic, and anti-fibrotic effects. We investigated the effect of recombinant human HGF (rh-HGF) on the development of both hepatocellular carcinoma (HCC) and preneoplastic nodules in rats fed a choline-deficient L-amino acid-defined (CDAA) diet, an animal model of hepatocarcinogenesis resembling human development of HCC with cirrhosis. From weeks 13 to 48 of the CDAA diet, rh-HGF (0.1 or 0.5 mg/kg/day) was administered intravenously to rats in four-week cycles, with treatment for five consecutive days of each week for two weeks, followed by a two-week washout period. Treatment with rh-HGF significantly inhibited the development of preneoplastic nodules in a dose-dependent manner at 24 weeks. Although the numbers and areas of the preneoplastic nodules in rats treated with rh-HGF were equivalent to those in mock-treated rats by 60 weeks, the incidence of HCC was reduced by HGF treatment. Although one rat treated with low-dose rh-HGF exhibited a massive HCC, which occupied almost the whole liver, and lung metastases, HGF treatment did not increase the overall frequency of HCC. Administration of high-dose rh-HGF, however, induced an increase in the urinary excretion of albumin, leading to decreased serum albumin at 60 weeks. These results indicate that long-term administration of rh-HGF does not accelerate hepatocarcinogenesis in rats fed a CDAA diet. However, these findings do not completely exclude the potential of HGF-induced hepatocarcinogenesis; this issue must be resolved before rh-HGF can be used for patients with intractable liver diseases, especially those with cirrhosis.
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Effect of hepatocyte growth factor on endogenous hepatocarcinogenesis in rats fed a choline-deficient L-amino acid-defined diet（共著） 査読あり
Nakanishi Chihiro 他
Oncology Reports 16 25 - 31 2006年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Repeated intravenous injection of recombinant human hepatocyte growth factor ameliorates liver cirrhosis but causes albuminuria in rats（共著） 査読あり
Kusumoto Kazunori 他
International Journal of Molecular Medicine 17 503 - 509 2006年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Repeated intravenous injection of recombinant human hepatocyte growth factor ameliorates liver cirrhosis but causes albuminuria in rats.
Kusumoto K, Ido A, Moriuchi A, Katsura T, Kim I, Takahama Y, Numata M, Kodama M, Hasuike S, Nagata K, Uto H, Inui K, Tsubouchi H
International journal of molecular medicine 17 ( 3 ) 503 - 9 2006年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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UTO Hirofumi, HAYASHI Katsuhiro, KUSUMOTO Kazunori, HASUIKE Satoru, NAGATA Kenji, KODAMA Mayumi, IDO Akio, KOHARA Michinori, STUVER Sherri O., TSUBOUCHI Hirohito
Hepatology research : the official journal of the Japan Society of Hepatology 34 ( 1 ) 28 - 34 2006年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Spontaneous elimination of hepatitis C virus RNA in individuals with persistent infection in a hyperendemic area of Japan（共著） 査読あり
Uto hirofumi 他
Hepatology Research 34 28 - 34 2006年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Spontaneous elimination of hepatitis C virus RNA in individuals with persistent infection in a hyperendemic area of Japan.
Uto H, Hayashi K, Kusumoto K, Hasuike S, Nagata K, Kodama M, Ido A, Kohara M, Stuver SO, Tsubouchi H
Hepatology research : the official journal of the Japan Society of Hepatology 34 ( 1 ) 28 - 34 2006年1月
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UTO Hirofumi, IDO Akio, KUSUMOTO Kazunori, HASUIKE Satoru, NAGATA Kenji, HAYASHI Katsuhiro, YAMAGISHI Toshiya, GOHDA Eiichi, TSUBOUCHI Hirohito
Hepatology research : the official journal of the Japan Society of Hepatology 33 ( 4 ) 272 - 276 2005年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Uto H., Ido A., Kusumoto K., Hasuike S., Nagata K., Hayashi K., Yamagishi T., Gohda E., Tsubouchi H.
Hepatology Research 33 ( 4 ) 272 - 276 2005年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Hepatology Research
Measurement of serum human hepatocyte growth factor (HGF) by enzyme-linked immunosorbent assay (ELISA) is useful for the early diagnosis and prediction of prognosis of patients with acute liver failure (ALF). This ELISA methodology, however, is neither rapid nor convenient for use at the bedside. In this study, we have developed a rapid semi-quantitative immunochromatographic (IC) assay and evaluated its usefulness in assessing patients with acute hepatic injury. Only 100 μl of serum is required; the assay can be easily completed in 20 min. The values obtained using this novel assay correlated well with the values obtained using the standard ELISA protocol. In addition, the values obtained in the IC assay correlated with clinical course; increased serum HGF levels were associated with an increased frequency of ALF and death. These results indicate that this rapid semi-quantitative IC assay for HGF is useful for the early diagnosis of ALF and prediction of clinical outcome in acute hepatic injury. © 2005 Elsevier Ireland Ltd. All rights reserved.
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Hepatocyte growth factor accelerates the proliferation of hepatic oval cells and possibly promotes the differentiation in a 2-acetylaminofluorene/partial hepatectomy model in rats.
Hasuike S, Ido A, Uto H, Moriuchi A, Tahara Y, Numata M, Nagata K, Hori T, Hayashi K, Tsubouchi H
Journal of gastroenterology and hepatology 20 ( 11 ) 1753 - 61 2005年11月
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The peroxisome proliferator-activated receptor-gamma agonist, pioglitazone, inhibits fat accumulation and fibrosis in the livers of rats fed a choline-deficient, l-amino acid-defined diet.
Uto H, Nakanishi C, Ido A, Hasuike S, Kusumoto K, Abe H, Numata M, Nagata K, Hayashi K, Tsubouchi H
Hepatology research : the official journal of the Japan Society of Hepatology 32 ( 4 ) 235 - 42 2005年8月
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Hepatocyte growth factor facilitates the repair of large colonic ulcers in 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats.
Numata M, Ido A, Moriuchi A, Kim I, Tahara Y, Yamamoto S, Hasuike S, Nagata K, Miyata Y, Uto H, Tsubouchi H
Inflammatory bowel diseases 11 ( 6 ) 551 - 8 2005年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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NAGATA TSUBOUCHI Yoshiko, IDO Akio, UTO Hirofumi, NUMATA Masatsugu, MORIUCHI Akihiro, KIM Ildeok, HASUIKE Satoru, NAGATA Kenji, SEKIYA Toru, HAYASHI Katsuhiro, TSUBOUCHI Hirohito
Hepatology research : the official journal of the Japan Society of Hepatology 31 ( 2 ) 79 - 87 2005年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Molecular mechanisms of hereditary persistence of alpha-fetoprotein (AFP) in two Japanese families A hepatocyte nuclear factor-1 site mutation leads to induction of the AFP gene expression in adult livers.
Nagata-Tsubouchi Y, Ido A, Uto H, Numata M, Moriuchi A, Kim I, Hasuike S, Nagata K, Sekiya T, Hayashi K, Tsubouchi H
Hepatology research : the official journal of the Japan Society of Hepatology 31 ( 2 ) 79 - 87 2005年2月
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Hypermethylation of the wild-type ferrochelatase allele is closely associated with severe liver complication in a family with erythropoietic protoporphyria.
Onaga Y, Ido A, Uto H, Hasuike S, Kusumoto K, Moriuchi A, Numata M, Nagata K, Hori T, Hayashi K, Tsubouchi H
Biochemical and biophysical research communications 321 ( 4 ) 851 - 8 2004年9月
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Ido A., Uto H., Moriuchi A., Nagata K., Onaga Y., Onaga M., Hori T., Hirono S., Hayashi K., Tamaoki T., Tsubouchi H.
Cancer Research 61 ( 7 ) 3016 - 3021 2001年4月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cancer Research
We previously reported that the retroviral vector expressing the herpes simplex virus-thymidine kinase gene under the control of 0.3-kb human α-fetoprotein (AFP) gene promoter (AF0.3) provided the cytotoxicity to ganciclovir (GCV) in high-AFP-producing human hepatoma cells but not in low-AFP-producing cells. Therefore, specific enhancement of AFP promoter activity is likely to be required to induce enough cytotoxicity in low-AFP-producing hepatoma cells. In this study, we constructed a hybrid promoter, [HRE] AF, in which a 0.4-kb fragment of human vascular endothelial growth factor 5′-flanking sequences containing hypoxia-responsive element (HRE) was fused to AF0.3 promoter. By means of the reporter gene transfection assay, hypoxia-inducible transcriptions that were mediated by [HRE] AF promoter were detected in low- and non-AFP-producing human hepatoma cells, but not in nonhepatoma cells. When the herpes simplex virus-thymidine kinase gene controlled by [HRE]AF promoter was transduced into hepatoma and nonhepatoma cells by a retroviral vector, the exposure to 1% O 2 induced GCV cytotoxicity specifically in the hepatoma cells. Moreover, in nude mice bearing solid tumor xenografts, only the tumors consisting of the virus-infected hepatoma cells gradually disappeared by GCV administration. These results indicate that the hypoxia-inducible enhancer of the human vascular endothelial growth factor gene, which is directly linked to human AFP promoter, involves selective and enhanced tumoricidal activity in gene therapy for hepatocellular carcinoma.