Papers - OSHIMA Tatsuya
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Hirano S., Oshima T., Inada A., Tsuruda T.
ACS Applied Bio Materials 9 ( 7 ) 3564 - 3572 2026.3
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：ACS Applied Bio Materials
Amyloid protein aggregates pose a significant challenge due to their extreme insolubility and association with neurodegenerative diseases. To identify effective solvents for dissolving amyloid-forming proteins, a screening of deep eutectic solvents (DESs), including natural DESs (NADES), and related solvents was conducted. Aggregated lysozyme (LA) was reproducibly prepared under acidic and thermal conditions as a model of poorly soluble protein aggregates. Spectroscopic analyses using Thioflavin T fluorescence and circular dichroism (CD), along with morphological observations under polarized light and SEM, confirmed the formation of β-sheet-rich amyloid-like structures. The citric acid−glycerol DES showed the highest solubility for LA, outperforming other DESs and DMSO. Its performance was further enhanced by the addition of ∼5% water. This result also suggests a strong contribution of multivalent organic acids, such as citric and malic acid, to protein solubilization. CD analysis indicated that lysozyme recovered a native-like secondary structure, suggesting partial structural recovery after dissolution in this DES. These findings highlight the potential of DESs as effective media for dissolving protein aggregates and suggest their possible utility in protein refolding and related biotechnological applications, including potential future biomedical applications.
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Two-step selective extraction of Ga(III) and in(III) from hydrochloric acid media using different ketones Reviewed International coauthorship
Stankovic N., Oshima T.
Separation and Purification Technology 386 2025.12
Authorship：Corresponding author Publishing type：Research paper (scientific journal) Publisher：Separation and Purification Technology
Indium and gallium are critical metals with increasing demand in advanced electronics and photovoltaics but a limited primary supply. This work presents a two-step solvent extraction process using neutral ketones for their selective recovery from hydrochloric acid leachates. Gallium (Ga(III)) was first separated using 2-nonanone, achieving >99 % extraction at 5 mol·L<sup>−1</sup> HCl with <1 % indium (In(III)) co-extraction. In(III) was then recovered using MIBK, reaching 85 % in a single stage and up to 99.7 % after three stages. Stripping tests showed that dilute hydrochloric acid (0.01 mol·L<sup>−1</sup>) enabled >92 % In(III) recovery, while deionized water achieved >99 % across all O/A ratios. Ga(III) was quantitatively stripped at moderate HCl concentrations (0.5–1.0 mol·L<sup>−1</sup>) and reached approximately 89 % recovery with water at O/A = 2:1. The use of water eliminates acid neutralization steps, reduces chemical consumption, and produces low-ionic-strength solutions that are ideal for downstream recovery operations. Process reproducibility was confirmed by low standard deviation and RSD values (<3 %) under all tested conditions. The integrated two-step flowsheet thus provides a cost-effective, environmentally friendly alternative to traditional systems based on organophosphorus extractants or ionic liquids. Its closed-loop design enables solvent regeneration and aqueous phase recycling, offering a scalable and sustainable approach for Ga(III) and indium recovery from secondary resources and electronic waste.
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Oshima T., Iwakiri Y., Yokota H., Inada A.
ACS Sustainable Chemistry and Engineering 13 ( 24 ) 9110 - 9118 2025.6
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：ACS Sustainable Chemistry and Engineering
Selective recovery of gold from waste electrical and electronic equipment has attracted increasing attention. Au(III), which is present as tetrachloroauric acid (HAuCl<inf>4</inf>) in hydrochloric acid media, can be extracted by using various organic solvents, such as ketones and ethers. However, the decisive factors of the solvent for predicting Au(III) extractability have not been confirmed. In the present study, the relationship between the extraction percentage of Au(III) and the properties of the solvent was investigated for 79 types of solvents. Based on the relationships between the Hansen solubility parameters of the solvent and the extraction percentage of Au(III) in 5.0 M HCl with a threshold of 80%, the extractability was classified with 93.7% accuracy. To predict the Au(III) extractability, a machine learning model was constructed using the relationship between the molecular descriptors of the solvents obtained from RDKit as explanatory variables and the extraction percentage of Au(III) as the objective variable. By selection of the descriptors that showed a strong correlation with the Au(III) extraction percentage, the squared correlation coefficient (R<sup>2</sup>) of the test data for the nonlinear support vector regression model using 35 descriptors was 0.943. Machine learning was performed again with the addition of surface charge density descriptors obtained from the solvent structure using the conductor-like screening model for real solvents. The R<sup>2</sup> value of the test data for the nonlinear support vector regression model using 28 descriptors, including the hydrogen bond acceptor descriptor as an additional descriptor, was 0.899, which was slightly lower than that of the model with 35 descriptors. Experimental validation using six additional solvents confirmed the model’s accuracy, with predicted values closely matching the measured extraction percentages.
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Development of a Pillar[6]arene Carboxylic Acid Derivative for the Extraction of a Cationic Protein Reviewed
Y. Ikushima, T. Oshima
Biomacromolecules 26 ( 3 ) 1571 - 1579 2025.2
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Enhanced water dispersibility and permeability through a Caco-2 cell monolayer of β-cryptoxanthin extracted from kumquats by complexation with casein Reviewed
T. Oshima, K. Takahashi, A. Inada, M. Yamasaki, Y. Yamasaki, N. Eto
Food Chemistry 360 12982 2021.10
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Au(III) extraction using ketone compounds with physical properties superior to current commercial extractants Reviewed
T. Oshima, K. Miyake
AICHE Journal 67 e17214 2021.7
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Morita S., Machida K., Yamasaki Y., Sato M., Oshikawa H., Oshima T., Ogawa K., Nishiyama K., Tachibana H., Yamasaki M.
Bioscience Biotechnology and Biochemistry 90 ( 4 ) 561 - 570 2026.3
Language：English Publishing type：Research paper (scientific journal) Publisher：Bioscience Biotechnology and Biochemistry
We prepared nanovesicles (NVs) derived from broccoli using ultracentrifugation and evaluated their anti-inflammatory properties. Two distinct NV populations were isolated as precipitates from broccoli homogenates following centrifugation at 20 000 × g and 200 000 × g. These NVs contained RNAs, proteins, isothiocyanates, and chlorophylls. Dynamic light scattering analysis confirmed their nanoscale size. The NVs were internalized by RAW264 cells and significantly inhibited nitric oxide production and NF-κB pathway activation under lipopolysaccharide (LPS) stimulation. Comprehensive analysis of inflammatory cytokine expression revealed strong suppression of interleukin-6 (IL-6) by both NV types, which was further validated by ELISA. Additionally, IL-1β and tumor necrosis factor-α production were also reduced. Notably, the anti-inflammatory effects were partially attributed to small RNAs (<200 nt) present within the NVs. Collectively, these findings suggest that broccoli-derived NVs possess potent anti-inflammatory activity.
DOI： 10.1093/bbb/zbag009
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Design of Gold Extraction Solvents Using Machine Learning Models Reviewed
Tsunemi T., Oshima T., Yokota H., Kaneko H.
ACS Omega 11 ( 4 ) 6560 - 6570 2026.2
Publishing type：Research paper (scientific journal) Publisher：ACS Omega
Dibutyl carbitol (DBC) has been conventionally used for solvent extraction of Au(III), which exists as tetrachloroauric acid (HAuCl<inf>4</inf>), leached by chlorination. However, the water solubility of DBC (3 g/dm<sup>3</sup>) leads to organic contamination of the aqueous phase. To identify solvents with both high gold extractability and low water solubility, a model Y = f(X) was constructed to predict extractability and water solubility Y from molecular descriptors X of chemical structures using experimental data for various compounds. The charge density profile calculated with COSMO-RS was used as X in the extractability prediction model and genetic algorithm-based wavelength selection, which allows variable selection in regions, was applied. The constructed models showed high prediction accuracy, with correlation coefficients exceeding 0.9 for both extractability and water solubility. The inverse analysis of these models was used to search for solvents with both high extractability and low water solubility. Solvents including (−)-fenchone and 4-phenyl-2-butanone were identified as promising solvents exhibiting comparable extractability to DBC but lower water solubility.
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Taniguchi A., Oshima T., Inada A.
Journal of Food Science and Technology 2026
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Food Science and Technology
A solid dispersion of curcumin in cocoa butter was prepared. The dispersion was prepared by emulsification followed by lyophilization. The apparent solubility of curcumin was increased by formulation with cocoa butter. The formulation was dispersed in an aqueous medium as a hydrocolloid. The formulation increased the accumulation of curcumin in Caco-2 cells.
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Ohe K., Yamaguchi Y., Oshima T.
Proceedings of International Conference on Artificial Life and Robotics 462 - 465 2026
Publishing type：Research paper (scientific journal) Publisher：Proceedings of International Conference on Artificial Life and Robotics
Sericin is an underutilized biomass of hydrophilic protein removal during the refining process of silk yarn. Processing abundant functional groups such as hydroxyl, carboxyl, amide, and amino groups, sericin exhibits affinity for metal ions, making it a promising biosorbent. This study investigated the adsorption behavior of metal ions using sericin and glutaraldehyde-crosslinked sericin(Glu-sericin). Glu-sericin selectively adsorbed precious metals over Cu(II) in 0.01 M HCl. In contrast, sericin showed a red color change after Au(III) adsorption from 0.01 M and 0.1 M HCl, indicating the reduction of Au(III) to Au(0). These results show that sericin non-crosslinked treatment, both adsorption and reduction functions, making it a unique biomass material for gold recovery.
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Inada A., Hoshihara N., Oshima T.
Colloids and Surfaces A Physicochemical and Engineering Aspects 723 137412 2025.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Colloids and Surfaces A Physicochemical and Engineering Aspects
In this study, nanoparticles (NPs) were synthesized by the coordination-bond-driven self-assembly of two tripeptides, H-Trp-His-Phe-OH (WHF) and H-Trp-His-Tyr-OH (WHY), with zinc ions. In addition, a one-pot method was developed to synthesize NP complexes with the anticancer drug doxorubicin (DOX) for application as drug delivery carriers. DOX release from DOX@NP-Zn(WHF) and DOX@NP-Zn(WHY) was enhanced under pH conditions mimicking the tumor microenvironment compared to physiological conditions. Cell viability assays demonstrated that NP-Zn(WHF) and NP-Zn(WHY) carriers exhibited minimal toxicity, whereas DOX@NP-Zn(WHF) and DOX@NP-Zn(WHY) showed excellent anticancer activity against tumor cells in a DOX concentration-dependent manner. NP-Zn(WH-X) could be a promising new carrier for controlled drug delivery in cancer therapy.
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Cancer cell microenvironment-responsive bio-metal-organic frameworks for anticancer drug delivery Reviewed
Inada A., Yoshinaka H., Kikuchi M., Oshima T.
Jcis Open 19 100149 2025.10
Language：English Publishing type：Research paper (scientific journal) Publisher：Jcis Open
A glutathione (GSH)-responsive metal-organic framework (MOF) was developed using biocompatible L-cystine and Zn for targeted doxorubicin (DOX) delivery. This strategy enhances drug release in the microenvironment while minimizing systemic toxicity. Disulfide-containing MOFs were synthesized using L-cystine as the ligand and Zn as the metal node. A one-pot method was developed to encapsulate DOX during synthesis. The redox-responsive behavior of the resulting DOX@MOF-Zn(cystine) was evaluated under physiological and tumor-mimicking conditions. In vitro assays revealed that the disulfide bonds in L-cystine ligand facilitate MOF degradation in the presence of GSH, triggering DOX release. DOX@MOF-Zn(cystine) exhibited significantly enhanced release under conditions mimicking the tumor microenvironment (pH 5.4, 20 mM GSH) compared to physiological conditions (pH 7.4, no GSH). Cell viability assays demonstrated minimal toxicity for blank MOFs and strong DOX-dependent anticancer effects from DOX@MOF-Zn(cystine). These findings suggest DOX@MOF-Zn(cystine) is an effective GSH-responsive drug delivery system for targeted cancer therapy.
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Extraction of Amino Acid Esters Using a Pillar[6]arene Carboxylic Acid Derivative as a Novel Acidic Macrocyclic Compound Reviewed
Y. Ikushima,T. Oshima, A. Inada
32 ( 2 ) 79 - 87 2025.7
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Sequence optimization of lipid-modified amphiphilic tetrapeptides as anticancer drug carriers Reviewed
114 103768 2025.6
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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膜乳化法を利用して調製したβ-クリプトキサンチン製剤の水分散性および細胞蓄積性の評価 Reviewed
谷口 愛里沙, 越智 貴大, 大島 達也, 稲田 飛鳥, 赤木 剛, 山本 建次, 清水 正高
化学工学論文集 51 ( 1 ) 12 - 17 2025.1
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)
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Effect of Acid Treatment on a λ-MnO2 Granulated Adsorbent for Adsorptive Recovery of Lithium Reviewed
T. Oshima, Y. Kadogawa, K. Shiraishi, K. Ohe, S. Nishihama, K. Yoshizuka
Solvent Extraction and Ion Exchange 43 79 - 93 2024.12
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Comprehensive extraction study using N,N-dioctylthiodiglycolamic acid: effect of S donor on metal extraction Reviewed
K. Shimojo, I. Fujiwara, T. Saito, T. Oshima
Analytical Sciences 40 1429 - 1436 2024.5
Authorship：Last author Language：English Publishing type：Research paper (scientific journal)
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Kawano Ami, Yamasaki Masao, Oshima Tatsuya, Matsumoto Tomoko, Yamasaki Yumi
Nippon Shokuhin Kagaku Kogaku Kaishi 71 ( 5 ) 181 - 181 2024.5
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Society for Food Science and Technology
DOI： 10.3136/nskkk.71.181
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Separation of gallium(III) using 2-nonanone based on ion solvation in acidic chloride media Reviewed
Hashizume M., Oshima T., Inada A.
Separation and Purification Technology 335 126156 - 126156 2024.5
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Separation and Purification Technology
Gallium (Ga) is an indispensable electronic material for devices such as light-emitting diodes, and the demand for Ga is expected to increase. In this study, a comparative analysis was carried out on Ga(III) extraction by neutral ketones and ethers in the presence of highly concentrated hydrochloric acid (HCl). Generally, Ga(III) extractability was high using aliphatic and aromatic ketones. By comparison, Ga(III) extractability was lower using monoether compounds. High Ga(III) extractability was obtained using aromatic 1,2-diethers. Suitable solvents for Ga extraction were screened based on Hansen solubility parameters. 2-Nonanone (2-NON), an aliphatic ketone, was found to have a high extraction capacity (more than 95 mM) competitive with dibutyl carbitol. The physical properties of 2-NON a low water solubility (0.5 g dm−3), low viscosity (1.3 mPa·s), and a sufficiently high flash point (76 °C) are suitable for industrial processes. Ga(III) and Fe(III) were selectively extracted using 2-NON and other solvents. Ga(III) extracted using 2-NON was quantitatively stripped by contact with aqueous solutions with low HCl concentrations. 2-NON was used to separate Ga(III) from a mixed solution of metal ions to investigate recycling copper indium gallium diselenide solar cells. Ga(III) was selectively extracted from the mixture with over 90 % stripping. The concentrations of the metal ions in the stripping solutions were 1.0 mM for Cu(II), 9.9 mM for In(III), 56.7 mM for Ga(III), and 0.02 mM for Se(IV), suggesting that 2-NON can be used for Ga(III) separation.
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A Comparative Study of Extraction of Selenium(IV) Using Various Organic Solvents in Hydrochloric Acid Media Reviewed
T. Oshima, M. Hashizume, Y. Kawaguchi, N. Matsuo, K. Ohe, A. Inada
Solvent Extraction Research and Development, Japan 31 ( 2 ) 41 - 48 2024.4
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.15261/serdj.31.41