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Affiliation |
Faculty of Medicine School of Medicine Department of Medical Sciences, Cardiovascular Physiology |
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Associate Professor |
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External Link |
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NAKAJIMA Yuichi
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Degree 【 display / non-display 】
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博士（獣医学） ( 1999.3 北里大学 )
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学士（獣医学） ( 1995.3 北里大学 )
Research Areas 【 display / non-display 】
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Life Science / Clinical pharmacy
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Life Science / Experimental pathology
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Life Science / Physiology
Papers 【 display / non-display 】
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2-Deoxy-L-ribose inhibits the invasion of thymidine phosphorylase-overexpressing tumors by suppressing matrix metalloproteinase-9 Reviewed
Yuichi Nakajima, Misako Haraguchi, Tatsuhiko Furukawa, Masatatsu Yamamoto, Hayao Nakanishi, Masae Tatematsu, Shin-ichi Akiyama
International Journal of Cancer 119 ( 7 ) 1710 - 1716 2006.10
Language：English Publishing type：Research paper (scientific journal)
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Inhibition of metastasis of tumor cells overexpressing thymidine phosphorylase by 2-deoxy-L-ribose. Reviewed
Nakajima Y,Gotanda T,Uchimiya H,Furukawa T,Haraguchi M,Ikeda R,Sumizawa T,Yoshida H,Akiyama S
Cancer Research 64 ( 5 ) 1794 - 1801 2004.3
Language：English Publishing type：Research paper (scientific journal)
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Madhyastha H., Madhyastha R., Chakraborty E., Banerjee K., Shah K., Nakajima Y., Chauhan N.S., Sudhakaran S.L., Ohe K., Muthukaliannan G.K., Gopalakrishnan A.V., Maruyama M., Watanabe N.
International Journal of Molecular Sciences 24 ( 4 ) 2023.2
Language：English Publishing type：Research paper (scientific journal) Publisher：International Journal of Molecular Sciences
Currently, there is a great demand for the development of nanomedicine aided wound tissue regeneration via silver doped nanoceuticals. Unfortunately, very little research is being carried out on antioxidants-doped silver nanometals and their interaction on the signaling axis during the bio-interface mechanism. In this study, c-phycocyanin primed silver nano hybrids (AgcPCNP) were prepared and analyzed for properties such as cytotoxicity, metal decay, nanoconjugate stability, size expansion, and antioxidant features. Fluctuations in the expression of marker genes during cell migration phenomena in in vitro wound healing scenarios were also validated. Studies revealed that physiologically relevant ionic solutions did not exhibit any adverse effects on the nanoconjugate stability. However, acidic, alkali, and ethanol solutions completely denatured the AgcPCNP conjugates. Signal transduction RT2PCR array demonstrated that genes associated with NFĸB- and PI3K-pathways were significantly (p < 0.5%) altered between AgcPCNP and AgNP groups. Specific inhibitors of NFĸB (Nfi) and PI3K (LY294002) pathways confirmed the involvement of NFĸB signaling axes. In vitro wound healing assay demonstrated that NFĸB pathway plays a prime role in the fibroblast cell migration. In conclusion, the present investigation revealed that surface functionalized AgcPCNP accelerated the fibroblast cell migration and can be further explored for wound healing biomedical applications.
DOI： 10.3390/ijms24043184
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The emerging role of exosomes in innate immunity, diagnosis and therapy. Reviewed
Gangadaran P, Madhyastha H, Madhyastha R, Rajendran RL, Nakajima Y, Watanabe N, Velikkakath AKG, Hong CM, Gopi RV, Muthukalianan GK, Valsala Gopalakrishnan A, Jeyaraman M, Ahn BC
Frontiers in immunology 13 1085057 2022
Language：English Publishing type：Research paper (scientific journal)
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Chromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del Reviewed International coauthorship
Panchariya V., Bhati V., Madhyastha H., Madhyastha R., Prasad J., Sharma P., Sharma P., Harish , Saini M.K., Rajput V.D., Nakajima Y., Kothari S.L., Gour V.S.
Scientific Reports 11 ( 1 ) 4186 2021.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Scientific Reports
Extraction of biosurfactants from plants is advantageous than from microbes. The properties and robustness of biosurfactant derived from the mesocarp of Balanites aegyptiaca have been reported. However, the dark brown property of biosurfactant and lack of knowledge of its biocompatibility limits its scope. In the present work, the decolorization protocol for this biosurfactant was optimized using hydrogen peroxide. The hemolytic potential and biocompatibility based on cell toxicity and proliferation were also investigated. This study is the first report on the decolorization and toxicity assay of this biosurfactant. For decolorization of biosurfactant, 34 full factorial design was used, and the data were subjected to ANOVA. Results indicate that 1.5% of hydrogen peroxide can decolorize the biosurfactant most efficiently at 40 °C in 70 min at pH 7. Mitochondrial reductase (MTT) and reactive oxygen species (ROS) assays on M5S mouse skin fibroblast cells revealed that decolorized biosurfactant up to 50 µg/mL for 6 h had no significant toxic effect. Hemolysis assay showed ~ 2.5% hemolysis of human RBCs, indicating the nontoxic effect of this biosurfactant. The present work established a decolorization protocol making the biosurfactant chromatically acceptable. Biocompatibility assays confirm its safer use as observed by experiments on M5S skin fibroblast cells under in vitro conditions.
MISC 【 display / non-display 】
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Deciphering the molecular events during arsenic induced transcription signal cascade activation in cellular milieu. Reviewed
Madhyastha H., Madhyastha R., Nakajima Y., Maruyama M.
BioMetals 2018.2
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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2-Deoxy-D-ribose, a downstream mediator of thymidine phosphorylase, regulates tumor angiogenesis and progression.
Nakajima Y, Madhyastha R, Maruyama M.
Anticancer Agents Med Chemistry 9 ( 2 ) 239 - 245 2009.2
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Bentham Science Publishers
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Thymidine phosphorylase
Haraguchi M., Nakajima Y., Ikeda R., Furukawa T, Sumisawa T.and Akiyama S
Molecular Target Therapy of Cancer 3 ( 2 ) 32 - 39 2005.4
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：Medical Review
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The role of thymidine phosphorylase,an anglogenic enzyme,in tumor progression.
Akiyama S, Furukawa T, Sumizawa T, Takebayashi Y, Nakajima Y, Shimaoka S, Haraguchi M
Cancer Scienｃe 95 ( 11 ) 851 - 857 2004.11
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Blackwell Publishing Asia Pty Lｔｄ
Presentations 【 display / non-display 】
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Silver primed nutraceutical hybrids: cellular and hematological evaluation Invited International conference
Madhyastha H, Madhyastha r., Nakajima Y., Maruyama M.
BioTERM
Event date： 2019.11.30 - 2019.12.3
Language：English Presentation type：Oral presentation (invited, special)
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Phycocyanin hybridized silver conjugates in wound healing-cell toxicity evaluation Invited International conference
Madhyastha H, Madhyastha R., Nakajima Y., Maruyama M.
New Horizon in Biotechnology
Event date： 2019.11.20 - 2019.11.29
Language：English Presentation type：Oral presentation (invited, special)
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Potential of intracellular protein and its hydrolysates from epiphytic bacteria associated with brown algae, Sargassum sp. as anticancer agents
Nur Asmi 他
第70回 西日本生理学会 (宮崎県) 日本生理学会
Event date： 2019.11.1 - 2019.11.2
Language：English Presentation type：Oral presentation (general)
Venue：宮崎県
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Characterization of curcumin and isoniazid surface modified gold and silver nanoparticles with biomedical relevance
Akhela Umapathi 他
第70回 西日本生理学会 (宮崎県) 日本生理学会
Event date： 2019.11.1 - 2019.11.2
Language：English Presentation type：Oral presentation (general)
Venue：宮崎県
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Characterization of curcumin and isoniazid surface modified gold and silver nanoparticles with biomedical relevance.
Akhela Umapathi, Radha Madhyastha, Mandeep Singh, Vipul Bansal, Navya PN, Devendra Jain, Harishkumar Madhyastha, Yuichi Nakajima, Masugi Maruyama, Hemant Kumar Daima.
西日本生理学会
Event date： 2019.11.1 - 2019.11.2
Language：Japanese Presentation type：Oral presentation (general)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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細胞老化と個体老化へのチミジンホスホリラーゼの影響
Grant number：21K07392 2021.04 - 2024.03
日本学術振興会 科学研究費助成事業 基盤研究（C）（一般）
Authorship：Principal investigator
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腫瘍内微小環境における２－デオキシ－Ｄ－リボースの影響
Grant number：19790292 2007.04 - 2009.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
近年、腫瘍の形成・増悪度の亢進に対して、腫瘍組織内に存在する間質系細胞の関わり合いが研究され始めている。この腫瘍組織内に存在するマクロファージが産生するとされる２－デオキシ－Ｄ－リボースの影響を調べ、新しい抗腫瘍薬の開発につながる知見を見出す。
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FMR治療における病理、遺伝子発現機構からの検証に基づいた左房機能評価の意義の確立
Grant number：23K08239 2023.04 - 2027.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究（C）
Authorship：Coinvestigator(s)
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腫瘍増殖・浸潤・転移を抑制する新規薬剤の開発
Grant number：12217121 2000.10 - 2003.09
科学研究費補助金 特定領域研究
Authorship：Coinvestigator(s)
L-dRibがTPを発現している腫瘍の浸潤をin vitroでもin vivoでも阻止すること、L-dRibがTP発現腫瘍で発現の亢進しているMMP-1とMMP-9の発現を抑制することを示した。