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Affiliation |
Frontier Science Research Center Living thing of experiment support section resource field |
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KOSHIMOTO Chihiro
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Degree 【 display / non-display 】
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博士（農学） ( 1992.3 京都大学 )
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農学修士 ( 1988.3 京都大学 )
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農学士 ( 1986.3 京都大学 )
Research Areas 【 display / non-display 】
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Life Science / Laboratory animal science / リサーチバイオリソース
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Life Science / Animal production science
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Life Science / Developmental biology
Papers 【 display / non-display 】
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Rina Ukyo, Akio Shinohara, Chihiro Koshimoto, Goro A. Nagura-Kato, Seiji Ieiri, Yasuhiro Tsuzuki, Shinsuke H. Sakamoto
Scientific Reports 13 ( 1 ) 9518 2023.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC
Abstract
Social separation is thought to induce a strong stress response in social juvenile mammals, but little is known about how this response might vary throughout the development. The present study examines the long-term effects of early-life stress (ELS) induced by social separation on individual behaviors later in life using the social and precocious species Octodon degus. Four experimental groups were established a positive control group of mothers and siblings from six litters comprised the socially housed (SH) group, while pups from seven litters were randomly assigned to three treatments: pups experiencing no separation (NS) treatment while their siblings did; repeated bouts of consecutive separation (CS); intermittent separation (IS). We analyzed the effects of separation treatment on the frequency and duration of freezing, rearing and grooming behaviors. ELS was correlated with higher hyperactivity, and hyperactivity increased with more frequent separation. However, the behavioral trend of the NS group changed to hyperactive in long-term observation. The findings suggest that the NS group was indirectly affected by ELS. In addition, suggesting ELS acts to converge an individual’s behavioral tendencies in a certain direction.DOI： 10.1038/s41598-023-36745-6
Other Link： https://www.nature.com/articles/s41598-023-36745-6
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Uno Y., Matsubara K., Inoue J., Inazawa J., Shinohara A., Koshimoto C., Ichiyanagi K., Matsuda Y.
Cytogenetic and Genome Research 163 ( 1-2 ) 42 - 51 2023.11
Language：English Publishing type：Research paper (scientific journal) Publisher：Cytogenetic and Genome Research
Constitutive heterochromatin, consisting of repetitive sequences, diverges very rapidly; therefore, its nucleotide sequences and chromosomal distributions are often largely different, even between closely related species. The chromosome C-banding patterns of two Gerbillinae species, Meriones unguiculatus and Gerbillus perpallidus, vary greatly, even though they belong to the same subfamily. To understand the evolution of C-positive heterochromatin in these species, we isolated highly repetitive sequences, determined their nucleotide sequences, and characterized them using chromosomal and filter hybridization. We obtained a centromeric repeat (MUN-HaeIII) and a chromosome 13-specific repeat (MUN-EcoRI) from M. unguiculatus. We also isolated a centromeric/ pericentromeric repeat (GPE-MBD) and an interspersed-type repeat that was predominantly amplified in the X and Y chromosomes (GPE-EcoRI) from G. perpallidus. GPE-MBD was found to contain a 17-bp motif that is essential for binding to the centromere-associated protein CENP-B. This indicates that it may play a role in the formation of a specified structure and/or function of centromeres. The nucleotide sequences of the three sequence families, except GPE-EcoRI, were conserved only in Gerbillinae. GPE-EcoRI was derived from the long interspersed nuclear elements 1 retrotransposon and showed sequence homology throughout Muridae and Cricetidae species, indicating that the repeat sequence occurred at least in the common ancestor of Muridae and Cricetidae. Due to a lack of assembly data of highly repetitive sequences constituting heterochromatin in whole-genome sequences of vertebrate species published to date, the knowledge obtained in this study provides useful information for a deep understanding of the evolution of repetitive sequences in not only rodents but also in mammals.
DOI： 10.1159/000533716
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Yasunori Abe, Kensuke Toyama, Akio Shinohara, Goro A. Nagura-Kato, Yuki Ikai, Chihiro Koshimoto, Joshua M. Spin, Naohito Hato
Anatomical Science International 98 ( 3 ) 426 - 433 2023.7
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC
DOI： 10.1007/s12565-022-00698-z
Other Link： https://link.springer.com/article/10.1007/s12565-022-00698-z/fulltext.html
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Pivotal role of High-Mobility Group Box 2 in ovarian folliculogenesis and fertility. Reviewed International journal
Shinichiro Shirouzu, Naohiro Sugita, Narantsog Choijookhuu, Yu Yamaguma, Kanako Takeguchi, Takumi Ishizuka, Mio Tanaka, Fidya Fidya, Kengo Kai, Etsuo Chosa, Yoshihiro Yamashita, Chihiro Koshimoto, Yoshitaka Hishikawa
Journal of ovarian research 15 ( 1 ) 133 - 133 2022.12
Language：English Publishing type：Research paper (scientific journal)
BACKGROUND: High-Mobility Group Box 1 (HMGB1) and HMGB2 are chromatin-associated proteins that belong to the HMG protein family, and are involved in the regulation of DNA transcription during cell differentiation, proliferation and regeneration in various tissues. However, the role of HMGB2 in ovarian folliculogenesis is largely unknown. METHODS: We investigated the functional role of HMGB1 and HMGB2 in ovarian folliculogenesis and fertilization using C57BL/6 wild type (WT) and HMGB2-knockout (KO) mice. Ovarian tissues were obtained from WT and HMGB2-KO mice at postnatal days 0, 3, 7, and 2, 6 months of age, then performed immunohistochemistry, qPCR and Western blotting analyses. Oocyte fertilization capability was examined by natural breeding and in vitro fertilization experiments. RESULTS: In HMGB2-KO mice, ovary weight was decreased due to reduced numbers of oocytes and follicles. Natural breeding and in vitro fertilization results indicated that HMGB2-KO mice are subfertile, but not sterile. Immunohistochemistry showed that oocytes expressed HMGB2, but not HMGB1, in neonatal and adult WT ovaries. Interestingly, in HMGB2-KO ovaries, a compensatory increase in HMGB1 was found in oocyte nuclei of neonatal and 2-month-old mice; however, this was lost at 6 months of age. CONCLUSIONS: The depletion of HMGB2 led to alterations in ovarian morphology and function, suggesting that HMGB2 plays an essential role in ovarian development, folliculogenesis and fertilization.
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Translocator protein imaging with 18F-FEDAC-positron emission tomography in rabbit atherosclerosis and its presence in human coronary vulnerable plaques. Reviewed International journal
Kazunari Maekawa, Atsushi B Tsuji, Atsushi Yamashita, Aya Sugyo, Chietsugu Katoh, Minghui Tang, Kensaku Nishihira, Yoshisato Shibata, Chihiro Koshimoto, Ming-Rong Zhang, Ryuichi Nishii, Keiichiro Yoshinaga, Yujiro Asada
Atherosclerosis 337 7 - 17 2021.11
Language：English Publishing type：Research paper (scientific journal)
BACKGROUND AND AIMS: This study aimed to investigate whether N-benzyl-N-methyl-2-[7,8-dihydro-7-(2-[18F]fluoroethyl)-8-oxo-2-phenyl-9H-purin-9-yl]acetamide (18F-FEDAC), a probe for translocator protein (TSPO), can visualize atherosclerotic lesions in rabbits and whether TSPO is localized in human coronary plaques. METHODS: 18F-FEDAC-PET of a rabbit model of atherosclerosis induced by a 0.5% cholesterol diet and balloon injury of the left carotid artery (n = 7) was performed eight weeks after the injury. The autoradiography intensity of 18F-FEDAC in carotid artery tissue sections was measured, and TSPO expression was evaluated immunohistochemically. TSPO expression was examined in human coronary arteries obtained from autopsy cases (n = 16), and in human coronary plaques (n = 12) aspirated from patients with acute myocardial infarction (AMI). RESULTS: 18F-FEDAC-PET visualized the atherosclerotic lesions in rabbits as high-uptake areas, and the standard uptake value was higher in injured arteries (0.574 ± 0.24) than in uninjured arteries (0.277 ± 0.13, p < 0.05) or myocardium (0.189 ± 0.07, p < 0.05). Immunostaining showed more macrophages and more TSPO expression in atherosclerotic lesions than in uninjured arteries. TSPO was localized in macrophages, and arterial autoradiography intensity was positively correlated with macrophage concentration (r = 0.64) and TSPO (r = 0.67). TSPO expression in human coronary arteries was higher in AMI cases than in non-cardiac death, or in the vulnerable plaques than in early or stable lesions, respectively. TSPO was localized in macrophages in all aspirated coronary plaques with thrombi. CONCLUSIONS: 18F-FEDAC-PET can visualize atherosclerotic lesions, and TSPO-expression may be a marker of high-risk coronary plaques.
Books 【 display / non-display 】
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大・中・小動物実験プロトコール
越本知大・村上昇・他31名（ Role： Joint editor）
宮日文化情報センター 2016.3
Language：Japanese Book type：Textbook, survey, introduction
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猫の行動学
斉藤徹, 久原孝俊, 片平清昭, 村中志朗 監訳 （ Role： Joint translator , 第7章 ネコの摂食行動 pp255-296）
インターズ 2009.10
Language：Japanese Book type：Scholarly book
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Several contributors affecting mouse sperm cryopreservation
C. Koshimoto, P. Mazur（ Role： Joint author）
Hokuto Shobo 2001.10
Language：English Book type：Scholarly book
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小児における晩発性確定的影響；放射線の線源と影響 原子放射線の影響に関する国連科学委員会の総会に対する1993年報告書 科学的付属書 I
国連科学委員会監修、放射線医学総合研究所監訳（ Role： Joint translator , pp873-929）
実業公報社 1995.10
Language：Japanese Book type：Report
MISC 【 display / non-display 】
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Shirouzu S., Sugita N., Choijookhuu N., Yamaguma Y., Takeguchi K., Ishizuka T., Tanaka M., Fidya , Kai K., Chosa E., Yamashita Y., Koshimoto C., Hishikawa Y.
Journal of Ovarian Research 16 ( 1 ) 21 2023.12
Language：English Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：Journal of Ovarian Research
Following publication of the original article [1], the uthors identified an error in authorgroup. Author name Fidya Fidya should be Fidya. The original article has been corrected.
Presentations 【 display / non-display 】
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冷蔵サンプルの輸送事故を防ぐための簡便な輸送方法に関する検討
七條宏樹・増元圭三・泉 孝一・篠原明男・坂本信介・越本知大
第34回日本実験動物技術者協会九州支部研究発表会 (宮崎市) 日本実験動物技術者協会九州支部
Event date： 2014.10.25 - 2014.10.26
Language：Japanese Presentation type：Oral presentation (general)
Venue：宮崎市
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ヨーロッパモリネズミに観察される糖尿病様個体の選抜方法の改善
冨山小百合・加藤悟郎・七條宏樹・坂本信介・篠原明男・越本知大、
第32回九州実験動物研究会 (宮崎市) 九州実験動物研究会
Event date： 2014.10.25 - 2014.10.26
Language：Japanese Presentation type：Oral presentation (general)
Venue：宮崎市
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高脂血症を自然発症するヨーロッパモリネズミの血中脂質への自発運動の影響
猪口瑞穂・坂本信介・篠原明男・越本知大、
第32回九州実験動物研究会 (宮崎市) 九州実験動物研究会
Event date： 2014.10.25 - 2014.10.26
Language：Japanese Presentation type：Oral presentation (general)
Venue：宮崎市
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BALB/c系統マウスの休眠発現は成長初期の栄養獲得量により調節される
加藤悟郎・坂本信介・篠原明男・森田哲夫・越本知大、
第32回九州実験動物研究会 (宮崎市) 九州実験動物研究会
Event date： 2014.10.25 - 2014.10.26
Language：Japanese Presentation type：Oral presentation (general)
Venue：宮崎市
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野生アカネズミの盲腸内微生物は飼育により変化する
井上比加里・酒井悠輔・坂本信介・越本知大・篠原明男
日本哺乳類学会2014年度大会 (京都市) 日本哺乳類学会
Event date： 2014.9.4 - 2014.9.7
Language：Japanese Presentation type：Poster presentation
Venue：京都市
Awards 【 display / non-display 】
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日本哺乳類学会優秀ポスター賞
2013.9 日本哺乳類学会
井上比加里・酒井悠輔・坂本信介・森田哲夫・越本知大・篠原明男
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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九州実験動物研究会 山内半田賞
2012.11 九州実験動物研究会
酒井悠輔・坂本信介・森田哲夫・篠原明男・越本知大
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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日本哺乳類学会優秀ポスター賞
2010.9 日本哺乳類学会
坂本信介・鈴木惟司・篠原明男・越本知大
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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日本哺乳類学会優秀ポスター賞
2009.11 日本哺乳類学会
篠原明男・越本知大
Award type：Award from international society, conference, symposium, etc. Country：Taiwan, Province of China
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Biology of the Cell Young Investigator Awards
2007.7 Biology of the Cell
83) K. Edashige, S. Ohta, M. Tanaka, T. Kuwano, D. M. Valdez Jr., T. Hara, B. Jin, S. Takahashi, S. Seki, C. Koshimoto, and M. Kasai
Award type：Award from international society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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日本固有種と欧州種が並在する独創的な野生由来アカネズミ属バイオリソースの質的評価
2014.04 - 2018.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
日本固有種および欧州種のアカネズミ属2種を対象に実験室繁殖コロニーを確立し、それぞれの質的評価を行うことで新規バイオリソースを創成する
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温度センサーチャンネル制御による生殖細胞と胚の低温/高温障害の克服
2014.04
科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
環境温度の変動によって発現する細胞膜表面の低分子輸送チャンネルの発現メカニズムを解析し、その人為制御を介して、生殖細胞凍結保存時の冷却もしくは加温ストレスの低減を試みる。
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動物接種とゲノム解析の組み合わせによる画期的な難同定病原微生物診断法の開発
2013.04
科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
未同定感染症患者の検体を培地もしくは実験動物に接種して培養して増幅し、大規模ゲノム解析にて確定診断する一連のプロトコルの開発
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生命現象の多様性を科学する新しい研究基盤の開発：日本固有齧歯類の実験動物化の試み
2011.04 - 2014.03
科学研究費補助金
Authorship：Principal investigator
日本固有の野生齧歯類であるApodemus属二種の実験動物化による研究基盤の創出の試み
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内在性水チャンネルの人為誘導と開閉制御による哺乳動物卵子の耐凍性向上
2011.04 - 2014.03
科学研究費補助金 基盤研究(B)
Authorship：Coinvestigator(s)
哺乳類卵子（未受精卵）は受精卵とは異なる凍結特性を有している。そこで卵子細胞膜上に低分子耐凍剤を透過するチャンネルを人為誘導し、その開閉を制御することで耐凍性を改善し、卵子凍結法の改善を試みる。
Other research activities 【 display / non-display 】
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絶滅危惧種 オキナワトゲネズミ 棲息調査
2013.03
トゲネズミ属3種の内2種は哺乳類でほぼ唯一Y染色体を欠く清家って一期校を有し、その仕組みが破壊召されていないため、本種の実験動物化とiPS細胞自立を目指し、その生息調査と試料採取を行った
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絶滅危惧種 アマミトゲネズミ 棲息調査
2013.02
トゲネズミ属3種の内2種は哺乳類でほぼ唯一Y染色体を欠くせい決定機構を有し、その仕組みや進化が解明されていないため、本種の実験動物化とiPS細胞自立を目指し、その生息調査と試料採取を継続した
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絶滅危惧種 トクノシマトゲネズミ 棲息調査
2012.12
トゲネズミ属3種の内2種は哺乳類でほぼ唯一Y染色体を欠くせい決定機構を有し、その仕組みや進化が解明されていないため、本種の実験動物化とiPS細胞自立を目指し、その生息調査と試料採取を継続した
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絶滅危惧種 アマミトゲネズミ 棲息調査
2012.11
トゲネズミ属3種の内2種は哺乳類でほぼ唯一Y染色体を欠くせい決定機構を有し、その仕組みや進化が解明されていないため、本種の実験動物化とiPS細胞自立を目指し、その生息調査と試料採取を行った
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天然記念物ニホンカモシカ九州内合同調査
2012.01 - 2012.10
環境省委託ニホンカモシカ調査に参加