KOSHIMOTO Chihiro

写真a

Affiliation

Frontier Science Research Center Living thing of experiment support section resource field

Title

Professor

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Degree 【 display / non-display

  • 博士(農学) ( 1992.3   京都大学 )

  • 農学修士 ( 1988.3   京都大学 )

  • 農学士 ( 1986.3   京都大学 )

Research Areas 【 display / non-display

  • Life Science / Laboratory animal science  / リサーチバイオリソース

  • Life Science / Animal production science

  • Life Science / Developmental biology

 

Papers 【 display / non-display

  • Translocator protein imaging with <sup>18</sup>F-FEDAC-positron emission tomography in rabbit atherosclerosis and its presence in human coronary vulnerable plaques Reviewed

    Maekawa K., Tsuji A.B., Yamashita A., Sugyo A., Katoh C., Tang M., Nishihira K., Shibata Y., Koshimoto C., Zhang M.R., Nishii R., Yoshinaga K., Asada Y.

    Atherosclerosis   337   7 - 17   2021.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Atherosclerosis  

    Background and aims: This study aimed to investigate whether N-benzyl-N-methyl-2-[7,8-dihydro-7-(2-[18F]fluoroethyl)-8-oxo-2-phenyl-9H-purin-9-yl]acetamide (18F-FEDAC), a probe for translocator protein (TSPO), can visualize atherosclerotic lesions in rabbits and whether TSPO is localized in human coronary plaques. Methods: 18F-FEDAC-PET of a rabbit model of atherosclerosis induced by a 0.5% cholesterol diet and balloon injury of the left carotid artery (n = 7) was performed eight weeks after the injury. The autoradiography intensity of 18F-FEDAC in carotid artery tissue sections was measured, and TSPO expression was evaluated immunohistochemically. TSPO expression was examined in human coronary arteries obtained from autopsy cases (n = 16), and in human coronary plaques (n = 12) aspirated from patients with acute myocardial infarction (AMI). Results: 18F-FEDAC-PET visualized the atherosclerotic lesions in rabbits as high-uptake areas, and the standard uptake value was higher in injured arteries (0.574 ± 0.24) than in uninjured arteries (0.277 ± 0.13, p < 0.05) or myocardium (0.189 ± 0.07, p < 0.05). Immunostaining showed more macrophages and more TSPO expression in atherosclerotic lesions than in uninjured arteries. TSPO was localized in macrophages, and arterial autoradiography intensity was positively correlated with macrophage concentration (r = 0.64) and TSPO (r = 0.67). TSPO expression in human coronary arteries was higher in AMI cases than in non-cardiac death, or in the vulnerable plaques than in early or stable lesions, respectively. TSPO was localized in macrophages in all aspirated coronary plaques with thrombi. Conclusions: 18F-FEDAC-PET can visualize atherosclerotic lesions, and TSPO-expression may be a marker of high-risk coronary plaques.

    DOI: 10.1016/j.atherosclerosis.2021.10.003

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  • Testosterone interrupts binding of Neurexin and Neuroligin that are expressed in a highly socialized rodent, Octodon degus. Reviewed International journal

    Nan Yagishita-Kyo, Yuki Ikai, Tomoko Uekita, Akio Shinohara, Chihiro Koshimoto, Keisuke Yoshikawa, Kei Maruyama, Sosuke Yagishita

    Biochemical and biophysical research communications   551   54 - 62   2021.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    Octodon degus is said to be one of the most human-like rodents because of its improved cognitive function. Focusing on its high sociality, we cloned and characterized some sociality-related genes of degus, in order to establish degus as a highly socialized animal model in molecular biology. We cloned degus Neurexin and Neuroligin as sociality-related genes, which are genetically related to autism spectrum disorder in human. According to our results, amino acid sequences of Neurexin and Neuroligin expressed in degus brain, are highly conserved to that of human sequences. Most notably, degus Neuroligin4 is highly similar to human Neuroligin4X, which is one of the most important autism-related genes, whereas mouse Neuroligin4 is known to be poorly similar to human Neuroligin4X. Furthermore, our work also indicated that testosterone directly binds to degus Neurexin and intercepts intercellular Neurexin-Neuroligin binding. Moreover, it is of high interest that testosterone is another key molecule of the higher incidence of autism in male. These results indicated that degus has the potential for animal model of sociality, and furthermore may promote understanding toward the pathogenic mechanism of autism.

    DOI: 10.1016/j.bbrc.2021.03.015

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  • Equilibrium vitrification of mouse embryos using low concentrations of cryoprotectants. Reviewed International journal

    Juan Qiu, Ayumi Hasegawa, Keiji Mochida, Atsuo Ogura, Chihiro Koshimoto, Kazutsugu Matsukawa, Keisuke Edashige

    Cryobiology   98   127 - 133   2021.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    Previously, we developed a method for vitrification of mouse embryos in a near-equilibrium state using EFS35c, PB1 medium containing 35% (v/v) ethylene glycol, and 0.98 M sucrose. This method has advantages in both slow freezing and vitrification. However, since the vitrification solution in this method contains high concentrations of cryoprotectants and thus has high osmolality, the solution would injure oocytes and embryos with high sensitivity to chemical toxicity and high osmolality. In this study, we examined whether embryos could be vitrified in a near-equilibrium state using a solution containing low concentrations of cryoprotectants and thus with low osmolality. To investigate whether embryos were vitrified in a near-equilibrium state, 2-cell mouse embryos were vitrified with EDFS10/10a, PB1 medium containing 10% (v/v) ethylene glycol, 10% (v/v) DMSO, and 0.4 M sucrose, in liquid nitrogen, stored at -80 °C for 4-28 days, and warmed in water at 25 °C. The viability of the embryos was evaluated by the appearance of embryos after warming and developmental ability. When embryos were vitrified in liquid nitrogen using EDFS10/10a, the survival and developmental ability into blastocysts after storage at -80 °C for 7 days were high, indicating that embryos were vitrified in a near-equilibrium state. A high proportion of embryos vitrified with EDFS10/10a developed to term after transportation with dry ice, re-cooling in liquid nitrogen, and transfer to recipients. Therefore, new equilibrium vitrification developed in this study may be useful for oocytes and embryos that are highly sensitive to the toxicity of cryoprotectants and high osmolality.

    DOI: 10.1016/j.cryobiol.2020.11.015

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  • Evaluation index of epilepsy-like seizures observed in common degu (<i>Octodon degus</i>) Reviewed

    Ikai Yuki, Shinohara Akio, Nagura-Kato Goro A., Shichijo Hiroki, Koshimoto Chihiro

    Honyurui Kagaku (Mammalian Science)   61 ( 1 )   3 - 11   2021

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    Authorship:Last author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:The Mammal Society of Japan  

    <p>The common degu (<i>Octodon degus</i>), originally native to South America, is now widely used as an experimental animal owing to several unique characteristics: common degus are highly social and use vocal sounds as communication tools. Importantly, their brain function is more complex than other laboratory rodents, making them a good model in the field of neuroscience. We established a colony of laboratory common degus and observed epilepsy-like seizures. We subsequently analyzed the process, strength, and duration of seizures by video recording. We examined 35 video images from 16 individuals (7 females and 9 males). The seizures began as abnormalities in the body parts (step 1), and then systemic tonic-clonic seizure (step 2) was observed. Following step 2, a short period of stillness (step 3) usually occurred, and then systemic tonic seizure occurred again (step 4). Ultimately, stillness (step 5) continued until recovery. We found sex-based differences in the strength and duration of seizures and observed large individual differences as well. Our results suggest that common degus have the potential to be used as a disease model for epilepsy.</p>

    DOI: 10.11238/mammalianscience.61.3

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  • Equilibrium vitrification of mouse embryos at various developmental stages using low concentrations of cryoprotectants

    Juan QIU, Kazutsugu MATSUKAWA, Chihiro KOSHIMOTO, Keisuke EDASHIGE

    Journal of Reproduction and Development   67 ( 2 )   109 - 114   2021

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japanese Society of Animal Reproduction  

    <p> We previously developed a new vitrification method (equilibrium vitrification) by which two-cell mouse embryos can be vitrified in liquid nitrogen in a highly dehydrated/concentrated state using low concentrations of cryoprotectants. In the present study, we examined whether this method is effective for mouse embryos at multiple developmental stages. Four-cell embryos, eight-cell embryos, morulae, and blastocysts were vitrified with EDFS10/10a, 10% (v/v) ethylene glycol and 10% (v/v) DMSO in FSa solution. The FSa solution was PB1 medium containing 30% (w/v) Ficoll PM-70 plus 0.5 M sucrose. The state of dehydration/concentration was assessed by examining the survival of vitrified embryos after storage at –80°C. When four-cell embryos and eight-cell embryos were vitrified with EDFS10/10a in liquid nitrogen and then stored at –80°C, the survival rate was high, even after 28 days, with relatively high developmental ability. On the other hand, the survival of morulae and blastocysts vitrified in liquid nitrogen and stored at –80°C for four days was low. Therefore, morulae and blastocysts cannot be vitrified in a highly dehydrated/concentrated state using the same method as with two-cell embryos. However, when blastocysts were shrunken artificially before vitrification, survival was high after storage at –80°C for four days with high developmental ability. In conclusion, the equilibrium vitrification method using low concentrations of cryoprotectants, which is effective for two-cell mouse embryos, is also useful for embryos at multiple stages. This method enables the convenient transportation of vitrified embryos using dry ice.</p>

    DOI: 10.1262/jrd.2020-152

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Books 【 display / non-display

  • 大・中・小動物実験プロトコール

    越本知大・村上昇・他31名( Role: Joint editor)

    宮日文化情報センター  2016.3 

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    Language:Japanese Book type:Textbook, survey, introduction

  • 猫の行動学

    斉藤徹, 久原孝俊, 片平清昭, 村中志朗 監訳 ( Role: Joint translator ,  第7章 ネコの摂食行動 pp255-296)

    インターズ  2009.10 

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    Language:Japanese Book type:Scholarly book

  • Several contributors affecting mouse sperm cryopreservation

    C. Koshimoto, P. Mazur( Role: Joint author)

    Hokuto Shobo  2001.10 

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    Language:English Book type:Scholarly book

  • 小児における晩発性確定的影響;放射線の線源と影響 原子放射線の影響に関する国連科学委員会の総会に対する1993年報告書 科学的付属書 I

    国連科学委員会監修、放射線医学総合研究所監訳( Role: Joint translator ,  pp873-929)

    実業公報社  1995.10 

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    Language:Japanese Book type:Report

Presentations 【 display / non-display

  • 冷蔵サンプルの輸送事故を防ぐための簡便な輸送方法に関する検討

    七條宏樹・増元圭三・泉 孝一・篠原明男・坂本信介・越本知大

    第34回日本実験動物技術者協会九州支部研究発表会  (宮崎市)  日本実験動物技術者協会九州支部

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    Event date: 2014.10.25 - 2014.10.26

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎市  

  • ヨーロッパモリネズミに観察される糖尿病様個体の選抜方法の改善

    冨山小百合・加藤悟郎・七條宏樹・坂本信介・篠原明男・越本知大、

    第32回九州実験動物研究会  (宮崎市)  九州実験動物研究会

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    Event date: 2014.10.25 - 2014.10.26

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎市  

  • 高脂血症を自然発症するヨーロッパモリネズミの血中脂質への自発運動の影響

    猪口瑞穂・坂本信介・篠原明男・越本知大、

    第32回九州実験動物研究会  (宮崎市)  九州実験動物研究会

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    Event date: 2014.10.25 - 2014.10.26

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎市  

  • BALB/c系統マウスの休眠発現は成長初期の栄養獲得量により調節される

    加藤悟郎・坂本信介・篠原明男・森田哲夫・越本知大、

    第32回九州実験動物研究会  (宮崎市)  九州実験動物研究会

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    Event date: 2014.10.25 - 2014.10.26

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎市  

  • 野生アカネズミの盲腸内微生物は飼育により変化する

    井上比加里・酒井悠輔・坂本信介・越本知大・篠原明男

    日本哺乳類学会2014年度大会  (京都市)  日本哺乳類学会

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    Event date: 2014.9.4 - 2014.9.7

    Language:Japanese   Presentation type:Poster presentation  

    Venue:京都市  

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Awards 【 display / non-display

  • 日本哺乳類学会優秀ポスター賞

    2013.9   日本哺乳類学会  

    井上比加里・酒井悠輔・坂本信介・森田哲夫・越本知大・篠原明男

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 九州実験動物研究会 山内半田賞

    2012.11   九州実験動物研究会  

    酒井悠輔・坂本信介・森田哲夫・篠原明男・越本知大

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 日本哺乳類学会優秀ポスター賞

    2010.9   日本哺乳類学会  

    坂本信介・鈴木惟司・篠原明男・越本知大

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 日本哺乳類学会優秀ポスター賞

    2009.11   日本哺乳類学会  

    篠原明男・越本知大

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    Award type:Award from international society, conference, symposium, etc.  Country:Taiwan, Province of China

  • Biology of the Cell Young Investigator Awards

    2007.7   Biology of the Cell  

    83) K. Edashige, S. Ohta, M. Tanaka, T. Kuwano, D. M. Valdez Jr., T. Hara, B. Jin, S. Takahashi, S. Seki, C. Koshimoto, and M. Kasai

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    Award type:Award from international society, conference, symposium, etc.  Country:Japan

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Grant-in-Aid for Scientific Research 【 display / non-display

  • 日本固有種と欧州種が並在する独創的な野生由来アカネズミ属バイオリソースの質的評価

    2014.04 - 2018.03

    科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

    日本固有種および欧州種のアカネズミ属2種を対象に実験室繁殖コロニーを確立し、それぞれの質的評価を行うことで新規バイオリソースを創成する

  • 温度センサーチャンネル制御による生殖細胞と胚の低温/高温障害の克服

    2014.04

    科学研究費補助金  基盤研究(B)

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    環境温度の変動によって発現する細胞膜表面の低分子輸送チャンネルの発現メカニズムを解析し、その人為制御を介して、生殖細胞凍結保存時の冷却もしくは加温ストレスの低減を試みる。

  • 動物接種とゲノム解析の組み合わせによる画期的な難同定病原微生物診断法の開発

    2013.04

    科学研究費補助金  基盤研究(C)

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    未同定感染症患者の検体を培地もしくは実験動物に接種して培養して増幅し、大規模ゲノム解析にて確定診断する一連のプロトコルの開発

  • 生命現象の多様性を科学する新しい研究基盤の開発:日本固有齧歯類の実験動物化の試み

    2011.04 - 2014.03

    科学研究費補助金 

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    Authorship:Principal investigator 

    日本固有の野生齧歯類であるApodemus属二種の実験動物化による研究基盤の創出の試み

  • 内在性水チャンネルの人為誘導と開閉制御による哺乳動物卵子の耐凍性向上

    2011.04 - 2014.03

    科学研究費補助金  基盤研究(B)

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    哺乳類卵子(未受精卵)は受精卵とは異なる凍結特性を有している。そこで卵子細胞膜上に低分子耐凍剤を透過するチャンネルを人為誘導し、その開閉を制御することで耐凍性を改善し、卵子凍結法の改善を試みる。

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Other research activities 【 display / non-display

  • 絶滅危惧種 オキナワトゲネズミ 棲息調査

    2013.03

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    トゲネズミ属3種の内2種は哺乳類でほぼ唯一Y染色体を欠く清家って一期校を有し、その仕組みが破壊召されていないため、本種の実験動物化とiPS細胞自立を目指し、その生息調査と試料採取を行った

  • 絶滅危惧種 アマミトゲネズミ 棲息調査

    2013.02

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    トゲネズミ属3種の内2種は哺乳類でほぼ唯一Y染色体を欠くせい決定機構を有し、その仕組みや進化が解明されていないため、本種の実験動物化とiPS細胞自立を目指し、その生息調査と試料採取を継続した

  • 絶滅危惧種 トクノシマトゲネズミ 棲息調査

    2012.12

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    トゲネズミ属3種の内2種は哺乳類でほぼ唯一Y染色体を欠くせい決定機構を有し、その仕組みや進化が解明されていないため、本種の実験動物化とiPS細胞自立を目指し、その生息調査と試料採取を継続した

  • 絶滅危惧種 アマミトゲネズミ 棲息調査

    2012.11

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    トゲネズミ属3種の内2種は哺乳類でほぼ唯一Y染色体を欠くせい決定機構を有し、その仕組みや進化が解明されていないため、本種の実験動物化とiPS細胞自立を目指し、その生息調査と試料採取を行った

  • 天然記念物ニホンカモシカ九州内合同調査

    2012.01 - 2012.10

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    環境省委託ニホンカモシカ調査に参加