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Affiliation |
Faculty of Medicine School of Medicine Department of Social Medicine, Legal Medicine |
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Professor |
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YUKAWA Nobuhiro
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Degree 【 display / non-display 】
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博士（医学） ( 1992.11 宮崎医科大学 )
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医学士 ( 1985.3 宮崎医科大学 )
Research Areas 【 display / non-display 】
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Life Science / Forensics medicine
Papers 【 display / non-display 】
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A clear presentation of intracranial hypostasis on PMCT
Shinkawa N., Imada M., Azuma M., Shinkawa N., Yukawa N.
Journal of Forensic and Legal Medicine 97 102540 2023.7
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Forensic and Legal Medicine
Intracranial hypostasis is a common postmortem change evident on postmortem CT (PMCT), but can be readily misinterpreted as subdural hematoma by inexperienced physicians. Although PMCT is necessarily lacking contrast enhancement, we reconstructed hypostatic sinuses into three-dimensional images resembling the results of in vivo venography. This simple methodology facilitates easy recognition of intracranial hypostasis.
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A Suggested Mechanism for Green Discoloration of the Postmortem Brain.
Shinkawa N, Takahashi N, Yano K, Sawaguchi A, Sonoda A, Kakizaki E, Yukawa N
The American journal of forensic medicine and pathology 44 ( 2 ) 132 - 135 2023.3
Language：English Publishing type：Research paper (scientific journal) Publisher：American Journal of Forensic Medicine and Pathology
In the putrefied brain, the cortex and basal ganglia show dark-grayish to green discoloration due to sulfhemoglobin formed from hydrogen sulfide (H2S) produced by endogenous bacteria and hemoglobin. In this study, we propose and demonstrate another mechanism of green discoloration in the brain. The formalin-fixed brain of a cadaver donated for medical education with no putrefaction was used. Half of the brain was immersed in sodium hydrosulfide solution, to imitate the H2S produced by bacteria. This half showed greenish discoloration, mainly in the basal ganglia and cortex. The other half showed positive Perls' Prussian blue staining, mainly in the basal ganglia and cortex. The area of greenish discoloration due to H2S and the region positive for Perls' Prussian blue staining coincided. Tissue treatment with strong oxidizing agents is required to liberate heme iron. The positive Perls' Prussian blue staining in this study thus does not reflect heme iron. In conclusion, we considered that non-heme iron compounds physiologically present in the brain and H2S represent sources of putrefactive greenish discoloration in the brain.
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Immunohistochemical study of chicken fat clots: Investigation of the formation mechanism
Matsuda M., Murase T., Komaki M., Shinba Y., Mitsuma M., Abe Y., Yamashita H., Yukawa N., Ikematsu K.
Legal Medicine 61 102208 2023.3
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Legal Medicine
In forensic practice, the presence of chicken fat clots (CFCs) in the heart and/or large blood vessels of cadavers has been empirically used to estimate the time from the onset of fatal events to death. However, little scientific evidence of its significance exists, and the mechanism of its formation has not been elucidated. CFCs contain large amounts of leukocytes; thus, we hypothesized that leukocytes might contribute to their formation. Since leukocytes, especially neutrophils, are considered to be involved in blood coagulation through the formation of neutrophil extracellular traps (NETs), we aimed to investigate whether NETs are related to the formation of CFCs through immunohistochemistry. Most cells in the CFCs were myeloperoxidase- and neutrophil elastase-positive, strongly suggesting that they were neutrophils. Since chromatin is released extracellularly during NET formation, immunostaining was performed against some types of histones in CFCs. A certain number of neutrophils in CFCs showed positive extra-nuclear and extracellular signals of histones. In addition, citrullination of histone H3, which is considered important for histone release, was immunohistochemically detected in some neutrophils. These results suggest that neutrophils may affect the formation of CFCs through histone release. Although it was not clear how and when citrullination and extracellular release of histones in CFCs occur in this study, our findings provide insights into the events occurring at the time of death in a human body.
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Shinkawa N., Kakizaki E., Sonoda A., Yukawa N.
Radiology Case Reports 18 ( 3 ) 1190 - 1196 2023.3
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Radiology Case Reports
We describe herein an autopsy case involving fatal hemorrhagic shock due to idiopathic sigmoid colonic intramural hematoma rupturing into the abdominal cavity. Antemortem computed tomography revealed a mass lesion in the sigmoid colon and a large amount of hemoperitoneum. On forensic autopsy, intramural hematoma of the sigmoid colon with ruptured serosa was identified, while the mucosa remained intact. Microscopically, hematomas were apparent, mainly in the muscularis propria. We diagnosed the cause of death as hemorrhagic shock due to idiopathic intramural hematoma of the sigmoid colon rupturing into the abdominal cavity. Although the patient had been receiving peritoneal dialysis, no relationship was identified between dialysis catheters and sigmoid colon intramural hematoma rupture. On computed tomography, the mass lesion was initially considered a submucosal neoplastic lesion or endometriotic lesion. Intramural hematoma should be considered as a differential diagnosis.
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[Letter to Editor] On Baecchi's staining method with reference to Nakagawa et al. (2021): “Advantages of filtration method for sperm-DNA genotyping in sexual assault cases” Reviewed
Yukawa N., Shinkawa N., Yano K., Tachibana M., Kakizaki E., Sonoda A.
Legal Medicine 2023.2
Authorship：Lead author Publishing type：Research paper (scientific journal)
Books 【 display / non-display 】
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Esentials of Autopsy Practice. Vol. 5 Innovations, Updates and Advances in Practice. 分担章の題名: Diatom and Laboratory Tests to Support a Conclusion of Death by Drowning.（共著）
Yukawa N, Kakizaki E, Kozawa S（ Role： Joint author , 内容に係わる解剖をし、執筆した。）
Springer-Verlag (London) 2012.10
Language：English Book type：Scholarly book
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Mitochondrial analysis in forensic science. In Rapley R, Whitehouse D, ediotrs.
Matsuda H, Yukawa N.（ Role： Joint author , 14）
John Weiley & Sons, Ltd 2007.4
Language：English Book type：Scholarly book
MISC 【 display / non-display 】
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特集 次世代シーケンサーが可能にした感染学の新しい展開 Invited
柿崎英二,湯川修弘
化学療法の領域 2017.6
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Presentations 【 display / non-display 】
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Degree of reduction を用いたアルコール代謝の法医学教育
湯川修弘、新川慶明、林 里采、園田 愛、柿崎英二
第70回日本法医学会学術九州地方集会
Event date： 2020.10.16 - 2020.10.17
Presentation type：Oral presentation (general)
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血液水溶液への可視光照射によるルミノール反応の加速効果
林 里采，白上 努，柿崎英二，新川慶明，園田 愛，湯川修弘
第69回日本法医学会学術九州地方集会
Event date： 2019.10.25 - 2019.10.26
Language：Japanese Presentation type：Oral presentation (general)
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ボタン電池の誤飲や嵌頓において，低電圧1.5 Vの電池でも組織傷害性のアルカリが生成される理由について
新川慶明，柿崎英二，林 里采，園田 愛，湯川修弘
第68回日本法医学会学術九州地方集会
Event date： 2018.10.19 - 2018.10.20
Language：Japanese Presentation type：Oral presentation (general)
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右手に笹の葉を固く握った状態で発見され即時性死体硬直と思われた溺死の一解剖例
湯川修弘、新川慶明、柿崎英二、園田 愛、林 里采、小片 守
第68回日本法医学会学術九州地方集会
Event date： 2018.10.19 - 2018.10.20
Presentation type：Oral presentation (general)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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溺死診断におけるプランクトン検査の意義：既成概念の大幅な変革に挑戦する
Grant number：22K19675 2022.04 - 2025.03
独立行政法人日本学術振興会 科学研究費補助金 挑戦的研究（萌芽）
園田 愛、
Authorship：Coinvestigator(s)
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最も少ない労力で行える溺死の補助診断検査：法医実務に合わせた最も効果的な活用法
Grant number：21H03215 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
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溺死の診断のためのLAMP法を用いた簡易迅速スクリーニング検査法の開発
2013.04 - 2016.03
科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
溺死の診断のためのLAMP法を用いた簡易迅速スクリーニング検査法の開発
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溺死体の血液及び諸臓器中に存在する水棲微生物のメタゲノム解析
2013.04 - 2016.03
科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
溺死体の血液及び諸臓器中に存在する水棲微生物のメタゲノム解析
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高度に腐乱した水中死体でも溺死の推定が行える検査法の開発
2008.04 - 2011.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
高度に腐乱した水中死体でも溺死の推定が行える検査法の開発
Available Technology 【 display / non-display 】
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教科書・文献のアルコールや中毒に関する化学反応式の点検
酸化数によらないアルコールやグルコースの代謝経路の俯瞰的理解
アセトアミノフェンの毒性機序についての再検討Home Page： 宮崎大学医学部医学科社会医学講座法医学分野
Related fields where technical consultation is available：大学教養課程レベルでの解糖やクエン酸回路など主要代謝経路の説明の仕方についてのアドバイス
Message：ライフサイエンス ボタン型電池の誤飲や鼻腔への嵌頓は小児や高齢者の緊急事態です。私たちは障がい機序（強アルカリ生成）と治療法についてインビトロの小実験と物理化学的な考察から理解を深めたいと思っています。