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Degree 【 display / non-display 】
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Doctor (Agriculture) ( 1991.3 Kagoshima University )
Research Areas 【 display / non-display 】
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Environmental Science/Agriculture Science / Science in plant genetics and breeding
Papers 【 display / non-display 】
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Rahman M.M., Mat K., Ishigaki G., Akashi R.
Animal science journal = Nihon chikusan Gakkaiho 92 ( 1 ) 2021.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Animal science journal = Nihon chikusan Gakkaiho
Year by year, huge quantities of by-products are generated during the manufacturing process of soybean-based products. Okara is one of the by-products, and it is an insoluble portion of the soybean. It consists of high moisture (8.4-22.9%); on dry matter basis, it contains high metabolizable energy (9.0-14.2 MJ/kg) and other components that include crude protein (20.9-39.1%), crude fiber (12.2-61.3%), crude fat (4.9-21.5%), and ash (3.4-5.3%). Fermentation of okara improves its nutritional quality and reduces its anti-nutrient contents. Due to animals' palatability, okara can be used to replace the soybean meal/concentrate feed partially or completely in ruminant's diet and partially in nonruminant's diet. Okara feeding does not depress the intake, digestibility, growth, milk production, blood metabolic profiles, and meat quality of animals. However, this by-product decays quickly due to its high moisture content, and its heavy weight and sticky nature make it difficult to process and expensive to dry using conventional methods. This paper thoroughly summarizes the utilization of okara as animal feed in the cause of developing a general guideline with favorable levels of inclusion in the diets of animals for its exploitation and valorization. This review will encourage further research to develop eco-friendly and value added feed for animals.
DOI： 10.1111/asj.13594
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Varietal differences in flavonoid and antioxidant activity in Japanese soybean accessions Reviewed
Arifin H.A., Hashiguchi T., Nagahama K., Hashiguchi M., Muguerza M., Sakakibara Y., Tanaka H., Akashi R.
Bioscience, biotechnology, and biochemistry 85 ( 4 ) 916 - 922 2021.3
Language：English Publishing type：Research paper (scientific journal) Publisher：Bioscience, biotechnology, and biochemistry
Soybean seeds contain many antioxidants, including flavonoids and other phytochemicals. Isoflavone is a phytoestrogen that mimics estrogenic effects on target tissues and also exerts antioxidant activity by sequestering free radicals. Despite many cultivars developed to date, varietal differences in flavonoid content and antioxidant activity in Japanese soybean accessions remain less well characterized. Here, we evaluated the seed content of isoflavones, total flavonoids, and total phenolics in 26 soybean accessions. Next, the antioxidant activity of each accession was examined using antioxidant responsive element (ARE) linked to a luciferase reporter in human HepG2 stable cells. The relative ARE luciferase activity rate of all soybean accessions varied up to 4-fold which ranged from 1.00 to 4.02; and 22 accessions exhibited significant antioxidant activities. Correlation analysis indicated that the level of total isoflavone moderately correlated with antioxidant activity.
DOI： 10.1093/bbb/zbaa104
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Quantitative analysis of seven plant hormones in Lotus japonicus using standard addition method Reviewed
Hashiguchi T., Hashiguchi M., Tanaka H., Fukushima K., Gondo T., Akashi R.
PLoS ONE 16 ( 2 February ) 2021.2
Language：English Publishing type：Research paper (scientific journal) Publisher：PLoS ONE
Plant hormones have been identified to be versatile signaling molecules essential for plant growth, development, and stress response. Their content levels vary depending on the species, and they also change in response to any external stimuli. Thus, simultaneous quantification of multiple plant hormones is required to understand plant physiology. Sensitive and quantitative analysis using liquid chromatography-linked mass spectrometry (LC-MS/MS) has been used in detecting plant hormones; however, quantification without stable isotopes is yet to be established. In this study, we quantified seven representative plant hormones of Lotus japonicus, which is a model legume for standard addition method. Accurate masses for monoisotopic ions of seven phytohormones were determined for high-resolution mass spectrometry (HR-MS). Selected ion monitoring (SIM) mode based on accurate masses was used in detecting phytohormones in the roots, stems, and leaves. Evaluation of matrix effects showed ion suppression ranging from 10.2% to 87.3%. Both stable isotope dilution and standard addition methods were able to detect plant hormones in the roots, stems, and leaves, with no significant differences in using both approaches and thus a standard addition method can be used to quantify phytohormones in L. japonicus. The method will be effective, especially when stable isotopes are not available to correct for matrix effects.
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Chotekajorn A., Hashiguchi T., Hashiguchi M., Tanaka H., Akashi R.
Plant Genetic Resources: Characterisation and Utilisation 19 ( 1 ) 35 - 43 2021.2
Language：English Publishing type：Research paper (scientific journal) Publisher：Plant Genetic Resources: Characterisation and Utilisation
Wild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): Asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.
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Kajiya-Kanegae H., Nagasaki H., Kaga A., Hirano K., Ogiso-Tanaka E., Matsuoka M., Ishimori M., Ishimoto M., Hashiguchi M., Tanaka H., Akashi R., Isobe S., Iwata H.
DNA research : an international journal for rapid publication of reports on genes and genomes 28 ( 1 ) 2021.1
Language：English Publishing type：Research paper (scientific journal) Publisher：DNA research : an international journal for rapid publication of reports on genes and genomes
We performed whole-genome Illumina resequencing of 198 accessions to examine the genetic diversity and facilitate the use of soybean genetic resources and identified 10 million single nucleotide polymorphisms and 2.8 million small indels. Furthermore, PacBio resequencing of 10 accessions was performed, and a total of 2,033 structure variants were identified. Genetic diversity and structure analysis congregated the 198 accessions into three subgroups (Primitive, World, and Japan) and showed the possibility of a long and relatively isolated history of cultivated soybean in Japan. Additionally, the skewed regional distribution of variants in the genome, such as higher structural variations on the R gene clusters in the Japan group, suggested the possibility of selective sweeps during domestication or breeding. A genome-wide association study identified both known and novel causal variants on the genes controlling the flowering period. Novel candidate causal variants were also found on genes related to the seed coat colour by aligning together with Illumina and PacBio reads. The genomic sequences and variants obtained in this study have immense potential to provide information for soybean breeding and genetic studies that may uncover novel alleles or genes involved in agronomically important traits.
Books 【 display / non-display 】
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形質転換プロトコール【植物編】「2章2-5セイヨウミヤコグサの形質転換プロトコール」
田中 秀典, 橋口 正嗣, 明石 良（ Role： Joint author）
化学同人，東京 2012.9
Language：Japanese Book type：Scholarly book
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形質転換プロトコール【植物編】「2章2-3バヒアグラスの形質転換プロトコール」
権藤 崇裕, 明石 良（ Role： Joint author）
化学同人，東京 2012.9
Language：Japanese Book type：Scholarly book
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形質転換プロトコール【植物編】「2章2-4ルジグラスの形質転換プロトコール」
石垣 元気, 明石 良（ Role： Joint author）
化学同人，東京 2012.9
Language：Japanese Book type：Scholarly book
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Field and Laboratory Methods for Grassland Science
（ Role： Joint author）
2004.3
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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NBRPミヤコグサ・ダイズにおけるバイオリソース整備 Invited
橋口 正嗣，田中 秀典，橋口 拓勇，明石 良
農業および園芸 96 ( 2 ) 153 - 162 2021.2
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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マメ科植物研究のためのミヤコグサバイオリソース Invited Reviewed
橋口正嗣，佐藤修正，橋口拓勇，田中秀典，明石 良
植物科学の最前線（BSJ-Review） 10 179 - 189 2019.7
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：日本植物学会
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Hashiguchi Masatsugu, Tanaka Hidenori, Abe Jun, Aoki Toshio, Anai Toyoaki, Suzuki Akihiro, Yamazaki Yukio, Akashi Ryo
Abstracts of Meeting of the CSSJ 242 ( 0 ) 2016
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：CROP SCIENCE SOCIETY OF JAPAN
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Akashi Ryo, Ishii Yasuyuki
Japanese Journal of Grassland Science 61 ( 1 ) 20 - 20 2015
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：Japanese Society of Grassland Science
DOI： 10.14941/grass.61.20
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Zoysia属における遺伝的多様性と進化に関する研究
田中 秀典, 橋口 正嗣, 北﨑 康生, 明石 良
「芝草研究」学会誌 41 137 - 143 2013.3
Language：Japanese Publishing type：Research paper, summary (national, other academic conference) Publisher：日本芝草学会
Presentations 【 display / non-display 】
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Fourth Phase National Bioresource Project (NBRP) in Japan -“Legumebase” for Lotus and Glycine Plant Research in Japan International conference
M. Hashiguchi, S. Sato, T. Hashiguchi, H. Tanaka, R. Akashi
Plant and Animal Genome Asia 2019 (Shenzhen, China)
Event date： 2019.6.6 - 2019.6.8
Language：English Presentation type：Poster presentation
Venue：Shenzhen, China
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ミヤコグサ関連リソースの最新状況
佐藤修正，二反田正悟，日下部翔平，Stig Andersen，Vikas Gupta，Nadia Kamal，Klaus Mayer，橋口正嗣，田中 秀典，明石良
第60回日本植物生理学会年会
Event date： 2019.3
Language：Japanese Presentation type：Poster presentation
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ミヤコグサの成長に関連する量的形質を推定する線形混合モデル
2. 長谷川 舞衣, 菊地 正隆, 小林 香織，市原 寿子, 佐藤 修正, 権藤 崇裕, 橋口 正嗣, 橋口拓勇，田中 秀典, 磯部 祥子, 明石 良, 中谷 明弘
第41回日本分子生物学会年会
Event date： 2018.12
Language：Japanese Presentation type：Poster presentation
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Phenotype substitute Environment (PE) value: Toward a G x E research in legumes International conference
4. Isobe S, Tanabata T, Hayashi A, Tanaka H, Hashiguchi M, Sato S, Nakaya A, Hasegawa M, Tanabata S and Akashi R
International Plant Phenotyping Symposium 2018
Event date： 2018.10
Language：English Presentation type：Oral presentation (general)
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マメ科植物における基礎から応用研 究のためのミヤコグサ・ダイズバイオリソース Invited
橋口 正嗣、佐藤 修正、橋口 拓勇、田中 秀典、明石 良
日本育種学会134回講演会
Event date： 2018.9.22 - 2018.9.24
Language：Japanese Presentation type：Symposium, workshop panel (nominated)
Awards 【 display / non-display 】
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草類における遺伝資源とその育種学的応用に関する研究
2020.3 日本草地学会
明石 良
Award type：International academic award (Japan or overseas) Country：Japan
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暖地型牧草を主とした飼料作物における組織培養とこれに基づく品質向上のための分子育種
1998.8 日本草地学会
明石 良
Award type：International academic award (Japan or overseas) Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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FOXハンティングシステムを用いたダイズのファンクショナルゲノミックス研究
Grant number：26450006 2014.04 - 2017.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
本研究は、遺伝子機能獲得型変異体の一つであるFOXハンティングシステム（Full-length over expressor gene hunting system)を用いてシロイヌナズナ由来完全長cDNAアグロライブラリーを西洋ミヤコグサ由来のユニークなべ根培養系（スーパールート）へランダムに導入するファンクショナルゲノミックスのための技術を確立するとともに、シロイヌナズナ由来の遺伝子が、マメ科植物における根の成長と分化および根粒形成に対して影響をもらたす新規な遺伝子の探索とそれを用いて生産性を向上させる分子育種を展開するための基盤整備と、有用遺伝子をダイズヘアグロバクテリウム法により導入し、その機能性を確認するものである。
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マメ科植物における遺伝子機能獲得型変異体を用いた根の特性に関する遺伝学的解析
Grant number：23580010 2011.04 - 2014.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
本研究は、遺伝子機能獲得型変異体の一つであるFOXハンティングシステム（Full-length over expressor gene hunting system)を用いてシロイヌナズナ由来完全長cDNAアグロライブラリーを西洋ミヤコグサ由来のユニークなべ根培養系（スーパールート）へランダムに導入するファンクショナルゲノミックスのための技術を確立するとともに、シロイヌナズナ由来の遺伝子が、マメ科植物における根の成長と分化および根粒形成に対して影響をもらたす新規な遺伝子の探索を行うものである。
本研究の成果は、ダイズ等マメ科作物の分子育種へ利用できるものと考えられる。 -
培養変異を利用した高消化性（飼料イネ）の作出
Grant number：13460119 2001.04 - 2004.03
科学研究費補助金 基盤研究(B)
本研究は、日本型イネ品種「日本晴れ」を用いて、種子由来カルスからの植物体再分化過程で出現する培養変異を利用することで、イネ品種における「高消化性イネ」を作出するものである。その結果、培養変異当代およびF2世代での消化性を確認したところ、通常に品種に比べ消化率が約10％向上した系統が見いだされた。
