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Faculty of Agriculture Region of Marine Life Science |
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HAYASHI Masahiro
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Degree 【 display / non-display 】
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Doctor(Agriculture) ( 1994.12 Osaka Prefecture University )
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Bachelor(Education) ( 1986.3 Kyoto University of Education )
Research Areas 【 display / non-display 】
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Life Science / Applied microbiology
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Life Science / Aquatic life science
Papers 【 display / non-display 】
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PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA Reviewed
Yohei Ishibashi, Hatsumi Goda, Rie Hamaguchi, Keishi Sakaguchi, Takayoshi Sekiguchi, Yuko Ishiwata, Yuji Okita, Seiya Mochinaga , Shingo Ikeuchi , Takahiro Mizobuchi , Yoshitake Takao, Kazuki Mori, Kosuke Tashiro, Nozomu Okino, Daiske Honda, Masahiro Hayashi, Makoto Ito
Communications Biology 2021.10
Language：English Publishing type：Research paper (scientific journal)
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Solitary and Synergistic Effects of Different Hydrophilic and Hydrophobic Phospholipid Moieties on Rat Behaviors Reviewed International coauthorship
Kikuchi S., Iwasaki Y., Yoshioka M., Hino K., Morita S.Y., Tada R., Uchimura Y., Kubo Y., Kobayashi T., Kinoshita Y., Hayashi M., Furusho Y., Tamiaki H., Ishiyama H., Kuroda M., Udagawa J.
Pharmaceutics 16 ( 6 ) 2024.6
Language：English Publishing type：Research paper (scientific journal) Publisher：Pharmaceutics
Glycerophospholipids have hydrophobic and hydrophilic moieties. Previous studies suggest that phospholipids with different moieties have different effects on rodent behavior; however, the relationship between chemical structures and behavioral effects remains unclear. To clarify the functions of phospholipid moieties, we injected male rats with phospholipids with different moieties and conducted behavioral tests. Exploratory activity was reduced by phosphatidylethanolamine (PE)(18:0/22:6) but not PE(18:0/18:0) or PE(18:0/20:4). Conversely, exploratory activity was increased by plasmanyl PE(16:0/22:6), which harbors an alkyl–ether linkage, but not by phosphatidylcholine (PC)(16:0/22:6) or plasmanyl PC(16:0/22:6). Docosahexaenoic acid (DHA)(22:6) and an alkyl–ether linkage in PE were thus postulated to be involved in exploratory activity. Anxiety-like behavior was reduced by plasmenyl PC(18:0/20:4), which harbors a vinyl–ether linkage, but not by PC(18:0/20:4) or plasmanyl PC(18:0/20:4), suggesting the anxiolytic effects of vinyl–ether linkage. The activation of social interaction was suppressed by PE(18:0/18:0), PE(18:0/22:6), PC(16:0/22:6), plasmanyl PE(16:0/22:6), and plasmanyl PC(16:0/22:6) but not by PE(18:0/20:4), plasmenyl PE(18:0/20:4), or plasmanyl PC(18:0/22:6). DHA may suppress social interaction, whereas arachidonic acid(20:4) or a combination of alkyl–ether linkage and stearic acid(18:0) may restore social deficits. Our findings indicate the characteristic effects of different phospholipid moieties on rat behavior, and may help to elucidate patterns between chemical structures and their effects.
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The Utilization of Heterotrophic Cultivation for Large Scale Production of Microalgae Invited Reviewed
HAYASHI Masahiro
31 ( 1 ) 4 - 7 2024.4
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)
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Fukuda M., Kinkawa M., Hayashi M.
Bioscience, Biotechnology and Biochemistry 88 ( 2 ) 206 - 211 2024.4
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Bioscience, Biotechnology and Biochemistry
Paramylon, a starch-like carbohydrate accumulated in Euglena gracilis cells, could be a potential source as a raw material for chemical products; its high-yield production would thus be highly desired. Although the molecular weight and polymerization degree of paramylon are important properties for its use as a raw material for chemical products, the available information about paramylon molecular weight remains insufficient. Therefore, in this study, we investigated a high-density E. gracilis culture approach and how culture conditions affect paramylon molecular weight. The nitrogen source, cultivation temperature, and nutrient feeding were optimized for maximum biomass and paramylon productivity. The maximum dry cell weight and paramylon content yields reached 108.9 g/L and 87.2%, respectively. Paramylon molecular weight was in the range of 220 000–320 000 Da. Our gel permeation chromatography analysis showed that the cells with a higher paramylon content tended to contain paramylon of a higher molecular weight.
DOI： 10.1093/bbb/zbad152
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Characterization of thraustochytrid-specific sterol O-acyltransferase: modification of DGAT2-like enzyme to increase the sterol production in Aurantiochytrium limacinum mh0186 Reviewed
石橋 洋平, 定光 翔平, 深堀 義朝, タノガシラ リン, 渡邉 昂, 林 雅弘, 伊東 信, 沖野 望
Applied and Environmental Microbiology 89 ( 11 ) 2023.11
Language：English Publishing type：Research paper (scientific journal) Publisher：American Society for Microbiology
Thraustochytrids are marine microorganisms expected to produce useful lipids. They synthesize polyunsaturated fatty acids and sterols and store them in lipid droplets as a form of triacylglycerol (TG) and sterol ester (SE), respectively. TG is synthesized by diacylglycerol O-acyltransferase (DGAT). There are several DGAT2 homologs in Aurantiochytrium limacinum. This study indicated that DGAT2C and DGAT2D are SE synthase and TG synthase, respectively, by disrupting their corresponding genes in A. limacinum mh0186. DGAT2C is revealed as thraustochytrid-specific acyl-CoA:sterol- O-acyltransferase by performing in vivo and in vitro assays after heterologous expression in Saccharomyces cerevisiae. DGAT2C and DGAT2D localized mainly to the endoplasmic reticulum (ER) and the lipid droplet, respectively, and the two of the N-terminal domains unique to DGAT2C were essential for ER localization and SE synthesis in A. limacinum mh0186. Interestingly, the study also found that deletion of the first eight transmembrane domains in the unique N-terminal region of DGAT2C increased SE and total sterol productivity when expressed in DGAT2C-deficient A. limacinum mh0186. In addition, intermediates such as Δ7-cholesterol (provitamin D3) also accumulated in SE by expression of the N-terminal-truncated DGAT2C, along with cholesterol and Δ7-stigmasterol, a major phytosterol in A. limacinum. This study proves that DGAT2C is an ER-localized SE synthase and that its suitable N-terminal deletion can increase sterol production in thraustochytrids.
Books 【 display / non-display 】
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藻類による二酸化炭素回収・固定化と有用物質生産
林 雅弘（ Role： Joint author , 従属栄養培養方式によるユーグレナの高密度培養）
シーエムシー出版 2025.1
Language：Japanese Book type：Scholarly book
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バイオマス材料の開発と応用
林 雅弘（ Role： Sole author , ユーグレナの産業利用ー特にパラミロンとそれを原料とするバイオプラスチックの開発）
株式会社 技術情報協会 2023.3
Language：Japanese Book type：Scholarly book
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カーボンニュートラル燃料最新動向
林 雅弘 松田綾子（ Role： Joint author , 藻類によるバイオ製造製造）
株式会社 情報機構 2022.7
Language：Japanese Book type：Scholarly book
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藻類培養技術
林 雅弘（ Role： Joint author , 藻類の濃縮と乾燥技術（ユーグレナの工業化を例に））
株式会社 情報機構 2021.10
Language：Japanese Book type：Scholarly book
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フードテックの最新動向
親泊政二三 林 雅弘（ Role： Joint author , 海産微生物ラビリンチュラの食品への利用）
シーエムシー出版 2021.7
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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微細藻類の大量培養における従属栄養培養の利用 Invited
林 雅弘 松田綾子
研究開発リーダー 20 ( 5 ) 37 - 42 2023.10
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：日本化学工学会
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Abe E., Ikeda K., Nutahara E., Hayashi M., Yamashita A., Taguchi R., Doi K., Honda D., Okino N., Ito M.
PLoS ONE 13 ( 8 ) 2018.8
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：PLoS ONE
© 2018 Abe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The titles and legends for Figs 2 and 3 are swapped. Please see the correct Figs 2 and 3 here. (Figure Presented).
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微細藻類プラスチックの開発
林 雅弘、芝上 基成
化学工学 80 ( 5 ) 2 - 4 2016.1
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：日本化学工学会
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油糧海洋微生物ラビリンチュラの産業利用
林 雅弘、松田綾子、松田高宜、泉 可也
酵素工学ニュース ( 74 ) 6 - 9 2015.10
Language：Japanese Publishing type：Research paper, summary (national, other academic conference) Publisher：酵素工学研究会
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油糧微生物ラビリンチュラ
林 雅弘
生物工学 93 ( 7 ) 426 - 429 2015.7
Language：Japanese Publishing type：Research paper, summary (national, other academic conference) Publisher：日本生物工学会
Presentations 【 display / non-display 】
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葉緑体欠損ユーグレナ Euglena gracilis SM-ZK株の高密度培養時における培養特性
FUKUDA Mizuki, HAYASHI Masahiro
日本農芸化学会2025年度大会 (北海道大学) 2025.3.4 日本農芸化学会
Event date： 2025.3.3 - 2025.3.8
Language：English Presentation type：Poster presentation
Venue：北海道大学
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インベルターゼによる廃糖蜜中のスクロース加水分解反応と Euglena gracilisによるパラミロン生産の並行複発酵の検討
山本 彩加 、林 雅弘 、泉 可也
日本農芸化学会2025年度大会 (北海道大学) 2025.3.4 日本農芸化学会
Event date： 2025.3.3 - 2025.3.8
Language：English Presentation type：Poster presentation
Venue：北海道大学
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従属栄養培養Euglena gracilisによる 嫌気的ワックスエステル生産に 好適な流加培養条件の検討とその代謝解析
金川真人・林雅弘
日本生物工学会2024年度九州支部大会 (シーガイアコンベンションセンター) 2024.12.1 マリンバイオテクノロジー学会
Event date： 2024.11.30 - 2024.12.1
Language：Japanese Presentation type：Oral presentation (general)
Venue：シーガイアコンベンションセンター
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Euglena gracilisによるパラミロン生産における並行複発酵の試み
山本彩加・福田美月・松田綾子 ・泉 可也・林 雅弘
日本生物工学会2024年度九州支部大会 (宮崎大学) 2024.12.1 日本生物工学会
Event date： 2024.11.30 - 2024.12.1
Language：Japanese Presentation type：Oral presentation (general)
Venue：宮崎大学
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非DNA組換えEPA生産性ラビリンチュラの特徴
下田匠平、松田綾子、関口峻允、本多大輔、林雅弘
第9回ラビリンチュラ・シンポジウム (九州大学医学部百年講堂) 2024.8.30
Event date： 2024.8.30
Language：Japanese Presentation type：Poster presentation
Venue：九州大学医学部百年講堂
Awards 【 display / non-display 】
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AgriFood SBIRピッチ2023 ビジネス構想優秀賞
2023.3 ⽣物系特定産業技術研究⽀援センター
林 雅弘
Award type：Award from Japanese society, conference, symposium, etc.
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日本農芸化学会2016年度大会トピックス賞
2016.4 日本農芸化学会
合田初美，持永聖也，濱口理恵，坂口圭史，伊東信，関口俊，石渡夕子，沖田裕司，本多大輔，林 雅弘
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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情動制御作用を有するプラズマローゲンのデザインと脳内輸送経路および標的細胞の 解明
Grant number：20K07918 2020.04 - 2023.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
Authorship：Coinvestigator(s)
Available Technology 【 display / non-display 】
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海洋微生物ラビリンチュラの産業利用
ユーグレナ（ミドリムシ）の産業利用
海洋マイクロプラスチック問題に関する要素技術の開発Related fields where technical consultation is available：新奇微生物の探索、育種、高密度培養や産業利用全般
Message： 新奇海洋微生物の探索のためなら、潜水作業もやります。
（本人及びスタッフ、院生は潜水士免許を取得）