HAYASHI Masahiro

写真a

Affiliation

Faculty of Agriculture Department of Marine Biological and Environmental Science

Title

Professor

External Link

Degree 【 display / non-display

  • Doctor(Agriculture) ( 1994.12   Osaka Prefecture University )

  • Bachelor(Education) ( 1986.3   Kyoto University of Education )

Research Areas 【 display / non-display

  • Life Science / Applied microbiology

  • Life Science / Aquatic life science

 

Papers 【 display / non-display

  • PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA Reviewed

    Yohei Ishibashi, Hatsumi Goda, Rie Hamaguchi, Keishi Sakaguchi, Takayoshi Sekiguchi, Yuko Ishiwata, Yuji Okita, Seiya Mochinaga , Shingo Ikeuchi , Takahiro Mizobuchi , Yoshitake Takao, Kazuki Mori, Kosuke Tashiro, Nozomu Okino, Daiske Honda, Masahiro Hayashi, Makoto Ito

    Communications Biology   2021.10

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    Language:English   Publishing type:Research paper (scientific journal)  

  • PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA

    Ishibashi Y., Goda H., Hamaguchi R., Sakaguchi K., Sekiguchi T., Ishiwata Y., Okita Y., Mochinaga S., Ikeuchi S., Mizobuchi T., Takao Y., Mori K., Tashiro K., Okino N., Honda D., Hayashi M., Ito M.

    Communications Biology   4 ( 1 )   2021.12

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Communications Biology  

    The demand for n-3 long-chain polyunsaturated fatty acids (n-3LC-PUFAs), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), will exceed their supply in the near future, and a sustainable source of n-3LC-PUFAs is needed. Thraustochytrids are marine protists characterized by anaerobic biosynthesis of DHA via polyunsaturated fatty acid synthase (PUFA-S). Analysis of a homemade draft genome database suggested that Parietichytrium sp. lacks PUFA-S but possesses all fatty acid elongase (ELO) and desaturase (DES) genes required for DHA synthesis. The reverse genetic approach and a tracing experiment using stable isotope-labeled fatty acids revealed that the ELO/DES pathway is the only DHA synthesis pathway in Parietichytrium sp. Disruption of the C20 fatty acid ELO (C20ELO) and ∆4 fatty acid DES (∆4DES) genes with expression of ω3 fatty acid DES in this thraustochytrid allowed the production of EPA and n-3docosapentaenoic acid (n-3DPA), respectively, at the highest level among known microbial sources using fed-batch culture.

    DOI: 10.1038/s42003-021-02857-w

    Scopus

  • C4-monomethylsterol β-glucoside and its synthase in Aurantiochytrium limacinum mh0186 Reviewed

    Endo I., Watanabe T., Miyamoto T., Monjusho-Goda H., Ohara J., Hayashi M., Hama Y., Ishibashi Y., Okino N., Ito M.

    Glycobiology   31 ( 10 )   1350 - 1363   2021.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Glycobiology  

    Thraustochytrids, unicellular marine protists, synthesize polyunsaturated fatty acids (PUFAs) and PUFA-containing phospholipids; however, little is known about their glycolipids and their associated metabolism. Here, we report two glycolipids (GL-A, B) and their synthases in Aurantiochytrium limacinum mh0186. Two glycolipids were purified from A. limacinum mh0186, and they were determined by gas chromatography, mass spectrometry and 2D nuclear magnetic resonance to be 3-O-β-D-glucopyranosyl-stigmasta-5,7,22-triene (GL-A) and 3-O-β-D-glucopyranosyl-4α-methyl-stigmasta-7,22-diene (GL-B), both of which are sterol β-glucosides (β-SGs); the structure of GL-B has not been reported thus far. Seven candidate genes responsible for the synthesis of these β-SGs were extracted from the draft genome database of A. limacinum using the yeast sterol β-glucosyltransferase (SGT; EC 2.4.1.173) sequence as a query. Expression analysis using Saccharomyces cerevisiae revealed that two gene products (AlSGT-1 and 2) catalyze the transfer of glucose from uridine diphosphate (UDP)-glucose to sterols, generating sterylglucosides (SGs). Compared to AlSGT-1, AlSGT-2 exhibited wide specificity for sterols and used C4-monomethylsterol to synthesize GL-B. The disruption of alsgt-2 but not alsgt-1 in strain mh0186 resulted in a decrease in the total SG and an almost complete loss of GL-B, indicating that AlSGT-2 is responsible for the synthesis of β-SGs in A. limacinum mh0186, especially GL-B, which possesses a unique sterol structure.

    DOI: 10.1093/glycob/cwab070

    Scopus

  • C4-monomethylsterol β-glucoside and its synthase in Aurantiochytrium limacinum mh0186 Reviewed

    Ikumi Endo, Takashi Watanabe, Tomofumi Miyamoto, Hatsumi Monjusho-Goda, Junichiro Ohara, Masahiro Hayashi, Yoichiro Hama, Yohei Ishibashi, Nozomu Okino, Makoto Ito

    Glycobiology   2021.7

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    Language:English   Publishing type:Research paper (scientific journal)  

  • A thraustochytrid-specific lipase/phospholipase with unique positional specificity contributes to microbial competition and fatty acid acquisition from the environment Reviewed

    Ishibashi Y., Aoki K., Okino N., Hayashi M., Ito M.

    Scientific Reports   9 ( 1 )   2019.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Scientific Reports  

    © 2019, The Author(s). Thraustochytrids are heterotrophic marine protists that are considered as important decomposers in the marine ecosystem; however, how they digest and uptake lipid nutrients from the environment is largely unknown. Genomic clustering analysis using thraustochytrid draft genome databases revealed that novel proteins with a Lipase_3 domain are commonly present in thraustochytrids, including Aurantiochytrium limacinum. After heterologous expression and His tag-based purification, protein ID: 145138 was identified as lipase/phospholipase capable of hydrolyzing triacylglycerol (TG) and phosphatidylcholine (PC). 145138 was secreted into the medium, and deletion of the 145138 gene in A. limacinum reduced the degradation of extracellular lipids. Fatty acids generated by 145138 were reused for the biosynthesis of PC and TG, and 145138 allowed A. limacinum to survive in the medium containing TG as a sole carbon source. 145138 hydrolyzed all the acyl-ester linkages of TG; however, the enzyme showed strict positional specificity toward phospholipids, generating 2-acyl lysophospholipids. The 2-acyl lysophospholipids showed stronger antimicrobial activity compared with 1-acyl lysophospholipids. These results suggested that 145138 is a bifunctional enzyme that contributes to the acquisition of lipid nutrients from the environment, as well as to generate antimicrobial lysophospholipids that are beneficial for competition with bacteria over lipid nutrients in the marine environment.

    DOI: 10.1038/s41598-019-52854-7

    Scopus

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Books 【 display / non-display

  • 藻類培養技術

    林 雅弘( Role: Joint author ,  藻類の濃縮と乾燥技術(ユーグレナの工業化を例に))

    株式会社 情報機構  2021.10 

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    Language:Japanese Book type:Scholarly book

  • 脂質・脂肪酸関連物質の使いこなしかたー素材開発・機能創生・応用技術ー

    林 雅弘・松田綾子( Role: Joint author)

    株式会社テクノシステム  2020.9 

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    Language:Japanese Book type:Scholarly book

  • Electron-Based Bioscience and Biotechnology

    Masahiro Hayashi and Ayako Matsuda( Role: Contributor)

    Springer  2020.9 

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    Language:English Book type:Scholarly book

  • セルロースナノファイバー製造・利用の最新動向

    芝上基成、林 雅弘( Role: Joint author)

    CMC  2019.6 

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    Total pages:42-56   Language:Japanese Book type:Scholarly book

  • 多文化共生社会に生きる : グローバル時代の多様性・人権・教育

    李 修京, 権 五定, 鷲山 恭彦

    明石書店  2019 

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    Language:Japanese

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MISC 【 display / non-display

  • Correction: Novel lysophospholipid acyltransferase plat1 of aurantiochytrium limacinum f26-b responsible for generation of palmitate-docosahexaenoate-phosphatidylcholine and phosphatidylethanolamine (PLoS ONE (2014) 9: 8 (e102377) DOI: 10.1371/journal.pone.0102377) Invited Reviewed

    Abe E., Ikeda K., Nutahara E., Hayashi M., Yamashita A., Taguchi R., Doi K., Honda D., Okino N., Ito M.

    PLoS ONE   13 ( 8 )   2018.8

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:PLoS ONE  

    © 2018 Abe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The titles and legends for Figs 2 and 3 are swapped. Please see the correct Figs 2 and 3 here. (Figure Presented).

    DOI: 10.1371/journal.pone.0203016

    Scopus

  • 微細藻類プラスチックの開発 Invited Reviewed

    林 雅弘、芝上 基成

    化学工学   80 ( 5 )   2 - 4   2016.1

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:日本化学工学会  

  • 微細藻類プラスチックの開発

    林 雅弘、芝上 基成

    化学工学   80 ( 5 )   2 - 4   2016.1

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)   Publisher:日本化学工学会  

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  • 油糧海洋微生物ラビリンチュラの産業利用

    林 雅弘、松田綾子、松田高宜、泉 可也

    酵素工学ニュース   ( 74 )   6 - 9   2015.10

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    Language:Japanese   Publishing type:Research paper, summary (national, other academic conference)   Publisher:酵素工学研究会  

  • 油糧海洋微生物ラビリンチュラの産業利用

    林 雅弘、松田綾子、松田高宜、泉 可也

    酵素工学ニュース   ( 74 )   6 - 9   2015.10

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)   Publisher:酵素工学研究会  

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Presentations 【 display / non-display

  • 魚粉/魚油代替飼料の開発 Invited

    林 雅弘

    SBIR研究発表会  2022.3.2 

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    Event date: 2022.3.2

    Language:Japanese   Presentation type:Oral presentation (keynote)  

  • 光合成なしで脱炭素は可能か? Invited

    林 雅弘

    OPERAシンポジウム   2022.2.28 

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    Event date: 2022.2.28

    Language:Japanese   Presentation type:Oral presentation (keynote)  

  • 微細藻類の産業利用とその課題 Invited

    林 雅弘

    情報機構講習会  2022.1.17 

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    Event date: 2022.1.17

    Language:Japanese   Presentation type:Oral presentation (keynote)  

  • ユーグレナナノファイバーの開発 Invited

    林 雅弘

    SBIRシンポジウム   2022.1.14 

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    Event date: 2022.1.14

    Language:Japanese   Presentation type:Oral presentation (keynote)  

  • 微細藻類の産業利用 Invited

    林 雅弘

    情報機構講演会  2021.12.24 

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    Event date: 2021.12.24

    Language:Japanese   Presentation type:Oral presentation (keynote)  

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Awards 【 display / non-display

  • 日本農芸化学会2016年度大会トピックス賞

    2016.4   日本農芸化学会  

    合田初美,持永聖也,濱口理恵,坂口圭史,伊東信,関口俊,石渡夕子,沖田裕司,本多大輔,林 雅弘

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

Grant-in-Aid for Scientific Research 【 display / non-display

  • 情動制御作用を有するプラズマローゲンのデザインと脳内輸送経路および標的細胞の 解明

    Grant number:20K07918  2020.04 - 2023.03

    科学研究費補助金  基盤研究(C)

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    Authorship:Coinvestigator(s)