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Faculty of Medicine School of Medicine Department of Anatomy, Ultrastructural Cell Biology |
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SAWAGUCHI Akira
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Degree 【 display / non-display 】
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博士（医学） ( 2001.3 宮崎医科大学 )
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学士（医学） ( 1996.3 宮崎医科大学 )
Research Areas 【 display / non-display 】
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Life Science / Cell biology
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Life Science / Anatomy
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Life Science / Morphology and anatomical structure
Papers 【 display / non-display 】
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KMnO4/Pb staining allows uranium free imaging of tissue architectures in low vacuum scanning electron microscopy Reviewed
Sawaguchi, A., Kamimura, T., Kitagawa, K., Nagashima, Y., Takahashi, N.
npj Imaging 2 40 2025.10
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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In situ strategy for biomedical target localization via nanogold nucleation and secondary growth. Reviewed International journal
Akira Sawaguchi, Takeshi Kamimura, Nobuyasu Takahashi, Atsushi Yamashita, Yujiro Asada, Hiroyuki Imazato, Fumiyo Aoyama, Akiko Wakui, Takeshi Sato, Narantsog Choijookhuu, Yoshitaka Hishikawa
Communications biology 4 ( 1 ) 710 - 710 2021.6
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)
Immunocytochemistry visualizes the exact spatial location of target molecules. The most common strategy for ultrastructural immunocytochemistry is the conjugation of nanogold particles to antibodies as probes. However, conventional nanogold labelling requires time-consuming nanogold probe preparation and ultrathin sectioning of cell/tissue samples. Here, we introduce an in situ strategy involving nanogold nucleation in immunoenzymatic products on universal paraffin/cryostat sections and provide unique insight into nanogold development under hot-humid air conditions. Nanogold particles were specifically localized on kidney podocytes to target synaptopodin. Transmission electron microscopy revealed secondary growth and self-assembly that could be experimentally controlled by bovine serum albumin stabilization and phosphate-buffered saline acceleration. Valuable retrospective nanogold labelling for gastric H+/K+-ATPase was achieved on vintage immunoenzymatic deposits after a long lapse of 15 years (i.e., 15-year-old deposits). The present in situ nanogold labelling is anticipated to fill the gap between light and electron microscopy to correlate cell/tissue structure and function.
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Informative three-dimensional survey of cell/tissue architectures in thick paraffin sections by simple low-vacuum scanning electron microscopy. Reviewed
Sawaguchi A., Kamimura T., Yamashita A., Takahashi N., Ichikawa K., Aoyama F., Asada Y.
Scientific Reports 8 ( 1 ) 7479 2018.12
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Oguri N., Gi T., Nakamura E., Maekawa K., Furukoji E., Okawa H., Kouyama S., Horiuchi S., Sawaguchi A., Sakae T., Azuma M., Asada Y., Yamashita A.
Research and Practice in Thrombosis and Haemostasis 9 ( 2 ) 102720 2025.2
Language：English Publishing type：Research paper (scientific journal) Publisher：Research and Practice in Thrombosis and Haemostasis
Background: Novel anticoagulants targeting coagulation factor (F)XI/activated FXI (FXIa) are currently under development. However, whether FXI is present in human deep vein thrombosis (DVT) and whether FXIa and activated FX (FXa) play different roles in venous thrombus formation and hemostasis remain unclear. Objectives: To determine the presence of FXI in DVT and the effects of direct oral FXIa and FXa inhibitors on venous thrombus formation and hemostasis in rabbits and on in vitro thrombus formation. Methods: We immunohistochemically assessed FXI localization in human-aspirated DVT (n = 15). Additionally, we compared thrombus formation induced by endothelial denudation and stenosis or stasis in the jugular vein and skin bleeding time and volume between rabbits treated with direct FXIa inhibitors (ONO-1600586) and FXa inhibitors (rivaroxaban). Ex vivo rabbit and human blood were perfused in a flow chamber under low-shear rates (70/s). Results: FXI was localized in all DVT, predominantly in fibrin-rich areas. The FXI immunopositive area in the nonorganizing area was greater than that in the organizing area. Although FXIa and FXa inhibitors comparably inhibited venous thrombus formation, FXIa inhibitors did not affect bleeding time or volume in rabbits. FXIa or FXa inhibitors mildly or strongly inhibited fibrin formation at low-shear rates, respectively. Furthermore, the FXIa inhibitor suppressed human FXIa activity, thrombin generation, and fibrin formation during perfusion. Conclusion: The pathologic findings of human DVT suggest FXI's role in human DVT. FXIa inhibitors may inhibit less fibrin formation than FXa inhibitors and may explain the minor role of FXIa in hemostasis.
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Kazuhiro Higuchi, Makoto Ikenoue, Takumi Ishizuka, Kengo Kai, Nobuyasu Takahashi, Toshiki Kubota, Shinichiro Shirouzu, Baljinnyam Lkham-Erdene, Kham Mo Aung, Michikazu Nakai, Akira Sawaguchi, Atsushi Nanashima, Yoshitaka Hishikawa
Acta histochemica et cytochemica 58 ( 1 ) 9 - 18 2025.1
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
SET domain bifurcated 1 (SETDB1), a histone H3K9-specific methyltransferase, is crucial for heterochromatin formation and intestinal homeostasis, but its role in intestinal ischemia-reperfusion injury (IRI) remains unclear. This study investigated changes in SETDB1-mediated nuclear chromatin regulation in intestinal epithelial cells (IECs) using an IRI mouse model. Jejunal samples were collected after 75 min of ischemia followed by 24 hr of reperfusion. Sinefungin was administered as a histone methyltransferase inhibitor. Morphologic changes were evaluated using hematoxylin-eosin staining and electron microscopy, and cell-adhesion molecule expression, including ZO-1, E-cadherin, integrin-β4, and laminin, was evaluated using immunohistochemistry. Super-resolution microscopy analyzed intranuclear SETDB1 localization and heterochromatin formation in IECs. IRI-affected jejunum exhibited massive IEC detachment, dilated intercellular spaces, basement membrane damage, and decreased expression of E-cadherin and integrin-β4. Sinefungin prevented these changes, however. The proportion of IECs expressing nuclear SETDB1 throughout the euchromatin was significantly higher in IRI-affected jejunum (77.8%) than sham-treated (3.0%) or sinefungin-treated, IRI-affected jejunum (2.7%). The proportion of IECs with decreased heterochromatin was significantly higher in sinefungin-treated, IRI-affected jejunum (84.3%) than untreated IRI-affected jejunum (15.6%). These findings suggest that SETDB1-mediated chromatin regulation is pivotal in intestinal IRI and represents a potential therapeutic target.
DOI： 10.1267/ahc.24-00061
Books 【 display / non-display 】
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Herring Nicole R, 荒川 高光, 池上 浩司, 浦川 将, 澤口 朗, 坂井 建雄（ Role： Joint translator）
丸善出版 2021 （ ISBN:9784621306284 ）
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Schünke Michael, Schulte Erik, Schumacher Udo, 青山 裕彦, 坂井 建雄, 大谷 修, 伊藤 正裕, 大谷 裕子, 大塚 愛二, 澤口 朗, 仙波 恵美子, 橋本 尚詞, 依藤 宏（ Role： Joint translator）
医学書院 2020 （ ISBN:9784260039277 ）
Language：Japanese Book type：Scholarly book
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ライフサイエンス顕微鏡学ハンドブック
澤口 朗（ Role： Contributor）
朝倉書店 2018.1
Language：Japanese
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標準生理学 第9版
澤口 朗（ Role： Contributor）
医学書院 2018.1
Language：Japanese
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Immunoelectron microscopy of cryofixed freeze substituted mammalian tissue culture cells. In. Immuno-electron Microscopy: Methods and Protocols.
Sawaguchi, A.（ Role： Sole author , 10）
Methods in Molecular Biology 2010.6
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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高圧凍結・凍結置換法による形態と組織化学
菅沼龍夫, 澤口 朗, 津山新一郎
電子顕微鏡 36 99 - 101 2001.1
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：中西印刷株式会社
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凍結技法における新展開
菅沼龍夫, 澤口 朗
細胞 35 32 - 35 2003.3
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：ニュー・サイエンス社
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高圧凍結技法の新展開－培養細胞への応用
澤口 朗, 菅沼 龍夫
顕微鏡 40 ( 3 ) 204 - 206 2005.12
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：中西印刷株式会社
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Remote image-sharing by the application of internet-video conference system to electron microscopy. ["jointly worked"]
50 ( 2 ) 137 - 139 2015.8
Language：Japanese Publishing type：Research paper, summary (national, other academic conference)
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Functional transformation of gastric parietal cells and intracellular trafficking of ion channels/transporters in the apical canalicular membrane associated with acid secretion.（共著）
Aoyama, F., Sawaguchi, A.
Biological & Pharmaceutical Bulletin 34 813 - 816 2011.6
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：日本薬学会
Presentations 【 display / non-display 】
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New informative three-dimensional survey of cell/tissue architectures in thick conventional paraffin sections by simple low-vacuum scanning electron microscopy. International conference
Sawaguchi, A., Kamimura, T., Yamashita, A., Takahashi, N., Ichikawa, K., Aoyama, F., Asada, Y.
2018 Cell Biology ASCB Annual Meeting
Event date： 2018.12.8 - 2018.12.12
Language：English Presentation type：Poster presentation
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グリオキサール系ホルマリン代替固定液・アルテフィックスⓇの短期および長期使用に関する評価
豊嶋（青山）典世、髙橋 伸育、澤口 朗
第74回日本解剖学会九州支部学術集会
Event date： 2018.10.27
Language：Japanese Presentation type：Oral presentation (general)
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ホルマリン代替固定液としてのアルテフィックスⓇの使用経験と組織学的検討
豊嶋（青山）典世、髙橋 伸育、澤口 朗
第59回日本組織細胞化学会総会・学術集会
Event date： 2018.9.29 - 2018.9.30
Language：Japanese Presentation type：Poster presentation
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低真空走査電子顕微鏡の特性を活かした厚切りパラフィン切片立体解析手法の開発と組織化学的応用
澤口 朗、高橋伸育、上村 健、市川 薫、山下 篤、豊嶋典世、浅田祐士郎
第59回日本組織細胞化学会総会・学術集会
Event date： 2018.9.29 - 2018.9.30
Language：Japanese Presentation type：Oral presentation (general)
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Informative Three-dimensional Survey of Cell/tissue Architectures in Thick Paraffin Sections by Simple Low-vacuum Scanning Electron Microscopy. International conference
Sawaguchi, A., Kamimura, T., Yamashita, A., Takahashi, N., Ichikawa, K., Aoyama, F., Asada, Y.
19th International Microscopy Congress
Event date： 2018.9.9 - 2018.9.14
Language：English Presentation type：Poster presentation
Awards 【 display / non-display 】
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日本解剖学会奨励賞
2004.8 日本解剖学会
澤口 朗
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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日本顕微鏡学会奨励賞
2007.5 日本顕微鏡学会
澤口 朗
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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日本組織細胞化学会 若手研究者学術奨励賞
2010.9 日本組織細胞化学会
澤口 朗
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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高圧凍結技法によるラット単離胃底腺粘液・漿液流動動態の組織化学的研究
Grant number：16790127 2004.04 - 2006.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
新しい凍結技法として注目される高圧凍結技法を応用したラット単離胃底腺における粘液・漿液流動動態の組織化学的研究
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高圧凍結技法を用いたラット単離胃粘膜・酸分泌後回復期壁細胞の膜動態研究
Grant number：18790145 2006.04 - 2008.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
新しい凍結技法として注目される高圧凍結技法を応用し、独自に開発したラット単離胃粘膜モデルにおける胃酸分泌後回復期にある壁細胞の膜動態に関する超微形態学的研究
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胃底腺壁細胞における胃酸分泌反復機構の解明をめざした細胞膜動態研究
Grant number：21790185 2009.04 - 2011.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
摂食後の酸分泌状態から休止状態に戻る胃底腺壁細胞が細胞膜とそれに付随するプロトンポンプをいかにして更新するかという未解明かつ重要な課題の全容解明を目指した超微形態学的研究
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新たな腰痛治療法の開発研究に資する深筋膜及び周辺組織の超微形態解析
Grant number：24K12379 2024.04 - 2027.03
日本学術振興会 科学研究費助成事業 基盤研究(C)
高橋 伸育, 澤口 朗
Authorship：Principal investigator
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Molecular mechanism of aortic aneurysm development
Grant number：19K08521 2019.04 - 2022.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
Tsuruda Toshihiro
Authorship：Principal investigator
This study aimed at evaluating the association between bone and abdominal aortic aneurysm using human aorta and mouse model. We focused on the receptor activator of nuclear factor kappaB ligand (RANKL) and osteoprotegerin (OPG). We found that OPG immunoreactivity was decreased in the medial layer of abdominal aortic aneurysm. Based on the finding, we administered the angiotensin II to wild-type (WT) and OPG knockout mice, and we found that OPG knockout mice exhibited the increased incidence of aortic rupture and dissection, compared with WT mice. The morphological changes were associated with the decreased medial and adventitial thickness, and increased the number of elastin fragmentation. On the other hand, recombinant OPG administration rescued the detrimental effects in these mice. This study suggests an important role for RANKL/OPG to preserve the aortic structure.
Available Technology 【 display / non-display 】
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iPS細胞由来血小板製剤開発と臨床応用に資する電子顕微鏡解析
標的生体物質の局在を可視化する新たな金ナノ粒子標識法の開発Related fields where technical consultation is available：医学生物学全般
Message：医学生物学分野における電子顕微鏡レベルの微細構造解析や、標的生体物質の局在解析に関するご相談に応じます。