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Affiliation |
Faculty of Medicine School of Medicine Department of Anatomy, Ultrastructural Cell Biology |
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SAWAGUCHI Akira
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Degree 【 display / non-display 】
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博士（医学） ( 2001.3 宮崎医科大学 )
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学士（医学） ( 1996.3 宮崎医科大学 )
Research Areas 【 display / non-display 】
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Life Science / Morphology and anatomical structure
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Life Science / Anatomy
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Life Science / Cell biology
Papers 【 display / non-display 】
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In situ strategy for biomedical target localization via nanogold nucleation and secondary growth. Reviewed International journal
Akira Sawaguchi, Takeshi Kamimura, Nobuyasu Takahashi, Atsushi Yamashita, Yujiro Asada, Hiroyuki Imazato, Fumiyo Aoyama, Akiko Wakui, Takeshi Sato, Narantsog Choijookhuu, Yoshitaka Hishikawa
Communications biology 4 ( 1 ) 710 - 710 2021.6
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)
Immunocytochemistry visualizes the exact spatial location of target molecules. The most common strategy for ultrastructural immunocytochemistry is the conjugation of nanogold particles to antibodies as probes. However, conventional nanogold labelling requires time-consuming nanogold probe preparation and ultrathin sectioning of cell/tissue samples. Here, we introduce an in situ strategy involving nanogold nucleation in immunoenzymatic products on universal paraffin/cryostat sections and provide unique insight into nanogold development under hot-humid air conditions. Nanogold particles were specifically localized on kidney podocytes to target synaptopodin. Transmission electron microscopy revealed secondary growth and self-assembly that could be experimentally controlled by bovine serum albumin stabilization and phosphate-buffered saline acceleration. Valuable retrospective nanogold labelling for gastric H+/K+-ATPase was achieved on vintage immunoenzymatic deposits after a long lapse of 15 years (i.e., 15-year-old deposits). The present in situ nanogold labelling is anticipated to fill the gap between light and electron microscopy to correlate cell/tissue structure and function.
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Makoto Ikenoue, Narantsog Choijookhuu, Koichiro Yano, Fidya, Nobuyasu Takahashi, Takumi Ishizuka, Shinichiro Shirouzu, Yu Yamaguma, Kengo Kai, Kazuhiro Higuchi, Akira Sawaguchi, Atsushi Nanashima, Yoshitaka Hishikawa
Histochemistry and Cell Biology 2024
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC
DOI： 10.1007/s00418-023-02263-9
Other Link： https://link.springer.com/article/10.1007/s00418-023-02263-9/fulltext.html
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Insertion sites of the muscles attached to the clavicle: a cadaveric study of the clavicle Reviewed
Imazato H., Takahashi N., Sawaguchi A., Hirakawa Y., Yamaguchi Y., Hiyoshi M., Tajima T., Chosa E.
BMC Musculoskeletal Disorders 24 ( 1 ) 160 2023.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：BMC Musculoskeletal Disorders
Background: Clavicle fractures are common injuries, especially in young, active individuals. Operative treatment is recommended for completely displaced clavicle shaft fractures, and plate fixation is stronger than the use of intramedullary nails. Few studies have reported on iatrogenic injuries to the muscle attached to the clavicle during fracture surgery. The aim of this study was to clarify the area of the insertion sites of muscles attached to the clavicle in Japanese cadavers using gross anatomy and three-dimensional (3D) analysis. We also aimed to compare the effects of anterior plate templating and superior plate templating on clavicle shaft fractures using 3D images. Methods: Thirty-eight clavicles from Japanese cadavers were analyzed. We removed all clavicles to identify the insertion sites and measured the size of the insertion area of each muscle. Three-dimensional templating was performed on both the superior and anterior plates of the clavicle using data obtained from computed tomography. The areas covered by these plates on the muscles attached to the clavicle were compared. Histological examination was performed on four randomly selected specimens. Results: The sternocleidomastoid muscle was attached proximally and superiorly; the trapezius muscle was attached posteriorly and partly superiorly; and the pectoralis major muscle and deltoid muscles were attached anteriorly and partially superiorly. The non-attachment area was located mainly in the posterosuperior part of the clavicle. It was difficult to distinguish the borders of the periosteum and pectoralis major muscles. The anterior plate covered a significantly broader area (mean 6.94 ± 1.36 cm2) of the muscles attached to the clavicle than did the superior plate (mean 4.11 ± 1.52 cm2) (p < 0.0001). On microscopy, these muscles were inserted directly into the periosteum. Conclusion: Most of the pectoralis major and deltoid muscles were attached anteriorly. The non-attachment area was located mainly from the superior to posterior part of the clavicle midshaft. Both macroscopically and microscopically, the boundaries between the periosteum and these muscles were difficult to demarcate. The anterior plate covered a significantly broader area of the muscles attached to the clavicle than that by the superior plate.
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Imazato H., Takahashi N., Hirakawa Y., Yamaguchi Y., Hiyoshi M., Tajima T., Chosa E., Sawaguchi A.
Scientific Reports 13 ( 1 ) 6352 2023.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Scientific Reports
Recent physiological studies have shown that the deep fascia has received much attention concerning clinical medicine; however, histological examination of the deep fascia has not been well established. In this study, we aimed to clarify and visualize the structure of the deep fascia by taking advantage of cryofixation techniques and low-vacuum scanning electron microscopy. As a result, the ultrastructural observations revealed three-dimensional stratification of the deep fascia composed of three layers: the first superficial layer consisting of collagen fibers extending in various directions with blood vessels and peripheral nerves; the second intermediate layer formed by single straight and thick collagen fibers with flexibility; and the third deepest layer, consisting of relatively straight and thin collagen fibers. We explored the use of two hooks to hold a piece of deep fascia in place through the course of cryo-fixation. A comparative observation with or without the hook-holding procedure would indicate the morphological adaptation to physiological stretch and contraction of the deep fascia. The present morphological approach paves the way to visualize three-dimensional ultrastructures for future biomedical studies including clinical pathophysiology.
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A Suggested Mechanism for Green Discoloration of the Postmortem Brain. Reviewed
Shinkawa N, Takahashi N, Yano K, Sawaguchi A, Sonoda A, Kakizaki E, Yukawa N
The American journal of forensic medicine and pathology 44 ( 2 ) 132 - 135 2023.3
Language：English Publishing type：Research paper (scientific journal) Publisher：American Journal of Forensic Medicine and Pathology
In the putrefied brain, the cortex and basal ganglia show dark-grayish to green discoloration due to sulfhemoglobin formed from hydrogen sulfide (H2S) produced by endogenous bacteria and hemoglobin. In this study, we propose and demonstrate another mechanism of green discoloration in the brain. The formalin-fixed brain of a cadaver donated for medical education with no putrefaction was used. Half of the brain was immersed in sodium hydrosulfide solution, to imitate the H2S produced by bacteria. This half showed greenish discoloration, mainly in the basal ganglia and cortex. The other half showed positive Perls' Prussian blue staining, mainly in the basal ganglia and cortex. The area of greenish discoloration due to H2S and the region positive for Perls' Prussian blue staining coincided. Tissue treatment with strong oxidizing agents is required to liberate heme iron. The positive Perls' Prussian blue staining in this study thus does not reflect heme iron. In conclusion, we considered that non-heme iron compounds physiologically present in the brain and H2S represent sources of putrefactive greenish discoloration in the brain.
Books 【 display / non-display 】
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Herring Nicole R, 荒川 高光, 池上 浩司, 浦川 将, 澤口 朗, 坂井 建雄（ Role： Joint translator）
丸善出版 2021 （ ISBN:9784621306284 ）
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Schünke Michael, Schulte Erik, Schumacher Udo, 青山 裕彦, 坂井 建雄, 大谷 修, 伊藤 正裕, 大谷 裕子, 大塚 愛二, 澤口 朗, 仙波 恵美子, 橋本 尚詞, 依藤 宏（ Role： Joint translator）
医学書院 2020 （ ISBN:9784260039277 ）
Language：Japanese Book type：Scholarly book
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ライフサイエンス顕微鏡学ハンドブック
澤口 朗（ Role： Contributor）
朝倉書店 2018.1
Language：Japanese
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標準生理学 第9版
澤口 朗（ Role： Contributor）
医学書院 2018.1
Language：Japanese
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Immunoelectron microscopy of cryofixed freeze substituted mammalian tissue culture cells. In. Immuno-electron Microscopy: Methods and Protocols.
Sawaguchi, A.（ Role： Sole author , 10）
Methods in Molecular Biology 2010.6
Language：Japanese Book type：Scholarly book
MISC 【 display / non-display 】
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高圧凍結・凍結置換法による形態と組織化学
菅沼龍夫, 澤口 朗, 津山新一郎
電子顕微鏡 36 99 - 101 2001.1
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：中西印刷株式会社
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凍結技法における新展開
菅沼龍夫, 澤口 朗
細胞 35 32 - 35 2003.3
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：ニュー・サイエンス社
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高圧凍結技法の新展開－培養細胞への応用
澤口 朗, 菅沼 龍夫
顕微鏡 40 ( 3 ) 204 - 206 2005.12
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：中西印刷株式会社
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Remote image-sharing by the application of internet-video conference system to electron microscopy. ["jointly worked"]
50 ( 2 ) 137 - 139 2015.8
Language：Japanese Publishing type：Research paper, summary (national, other academic conference)
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Functional transformation of gastric parietal cells and intracellular trafficking of ion channels/transporters in the apical canalicular membrane associated with acid secretion.（共著）
Aoyama, F., Sawaguchi, A.
Biological & Pharmaceutical Bulletin 34 813 - 816 2011.6
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：日本薬学会
Presentations 【 display / non-display 】
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New informative three-dimensional survey of cell/tissue architectures in thick conventional paraffin sections by simple low-vacuum scanning electron microscopy. International conference
Sawaguchi, A., Kamimura, T., Yamashita, A., Takahashi, N., Ichikawa, K., Aoyama, F., Asada, Y.
2018 Cell Biology ASCB Annual Meeting
Event date： 2018.12.8 - 2018.12.12
Language：English Presentation type：Poster presentation
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グリオキサール系ホルマリン代替固定液・アルテフィックスⓇの短期および長期使用に関する評価
豊嶋（青山）典世、髙橋 伸育、澤口 朗
第74回日本解剖学会九州支部学術集会
Event date： 2018.10.27
Language：Japanese Presentation type：Oral presentation (general)
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ホルマリン代替固定液としてのアルテフィックスⓇの使用経験と組織学的検討
豊嶋（青山）典世、髙橋 伸育、澤口 朗
第59回日本組織細胞化学会総会・学術集会
Event date： 2018.9.29 - 2018.9.30
Language：Japanese Presentation type：Poster presentation
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低真空走査電子顕微鏡の特性を活かした厚切りパラフィン切片立体解析手法の開発と組織化学的応用
澤口 朗、高橋伸育、上村 健、市川 薫、山下 篤、豊嶋典世、浅田祐士郎
第59回日本組織細胞化学会総会・学術集会
Event date： 2018.9.29 - 2018.9.30
Language：Japanese Presentation type：Oral presentation (general)
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Informative Three-dimensional Survey of Cell/tissue Architectures in Thick Paraffin Sections by Simple Low-vacuum Scanning Electron Microscopy. International conference
Sawaguchi, A., Kamimura, T., Yamashita, A., Takahashi, N., Ichikawa, K., Aoyama, F., Asada, Y.
19th International Microscopy Congress
Event date： 2018.9.9 - 2018.9.14
Language：English Presentation type：Poster presentation
Awards 【 display / non-display 】
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日本解剖学会奨励賞
2004.8 日本解剖学会
澤口 朗
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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日本顕微鏡学会奨励賞
2007.5 日本顕微鏡学会
澤口 朗
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
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日本組織細胞化学会 若手研究者学術奨励賞
2010.9 日本組織細胞化学会
澤口 朗
Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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高圧凍結技法によるラット単離胃底腺粘液・漿液流動動態の組織化学的研究
2004.04 - 2006.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
新しい凍結技法として注目される高圧凍結技法を応用したラット単離胃底腺における粘液・漿液流動動態の組織化学的研究
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高圧凍結技法を用いたラット単離胃粘膜・酸分泌後回復期壁細胞の膜動態研究
2006.04 - 2008.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
新しい凍結技法として注目される高圧凍結技法を応用し、独自に開発したラット単離胃粘膜モデルにおける胃酸分泌後回復期にある壁細胞の膜動態に関する超微形態学的研究
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胃底腺壁細胞における胃酸分泌反復機構の解明をめざした細胞膜動態研究
2009.04 - 2011.03
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
摂食後の酸分泌状態から休止状態に戻る胃底腺壁細胞が細胞膜とそれに付随するプロトンポンプをいかにして更新するかという未解明かつ重要な課題の全容解明を目指した超微形態学的研究
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Molecular mechanism of aortic aneurysm development
Grant number：19K08521 2019.04 - 2022.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
Tsuruda Toshihiro
Authorship：Principal investigator
This study aimed at evaluating the association between bone and abdominal aortic aneurysm using human aorta and mouse model. We focused on the receptor activator of nuclear factor kappaB ligand (RANKL) and osteoprotegerin (OPG). We found that OPG immunoreactivity was decreased in the medial layer of abdominal aortic aneurysm. Based on the finding, we administered the angiotensin II to wild-type (WT) and OPG knockout mice, and we found that OPG knockout mice exhibited the increased incidence of aortic rupture and dissection, compared with WT mice. The morphological changes were associated with the decreased medial and adventitial thickness, and increased the number of elastin fragmentation. On the other hand, recombinant OPG administration rescued the detrimental effects in these mice. This study suggests an important role for RANKL/OPG to preserve the aortic structure.
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Analysis of inter-phage interaction and its impacts on the evolution of enterohemorrhagic E. coli
Grant number：18K07116 2018.04 - 2021.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Nakamura Keiji
Authorship：Principal investigator
Bacteriophages (or phages) play major roles in the evolution of bacterial pathogens. Multiple phages are often integrated in a host chromosome as prophages, providing various possibilities for prophage-prophage interactions in bacterial cells. The present study investigated an inter-phage interaction between "phage in phage" newly identified in the enterohemorrhagic E. coli (EHEC) O145 strains, and revealed (i) that this coexistence of phages could affect phenotypes of the phages, ii) that phages integrating another prophage may be occurred in strains belonging to various serotypes of EHEC and enteropathogenic E. coli (EPEC), and iii) that this system possibly promote evolution as pathogenic bacteria and genetic diversity in host E. coli. These findings obtained here provide novel insights for not only the research communities working on EHEC/EPEC and their phages but also a wide range of researchers in basic and medical microbiology as well as bacterial evolution.
Available Technology 【 display / non-display 】
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iPS細胞由来血小板製剤開発と臨床応用に資する電子顕微鏡解析
標的生体物質の局在を可視化する新たな金ナノ粒子標識法の開発Related fields where technical consultation is available：医学生物学全般
Message：医学生物学分野における電子顕微鏡レベルの微細構造解析や、標的生体物質の局在解析に関するご相談に応じます。