Papers - KAWAHARA Satoshi
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Development of an industrial processing method for removing contaminating mucopolysaccharides from low molecular weight heparin preparations Reviewed
Murata H., Shimamura H., Matsuhisa A., Yamamoto K., Sugiyama K., Kawakami T., Yoden H., Uemura H., Toda T., Muguruma M., Kawahara S.
Nippon Shokuhin Kagaku Kougaku Kaishi 61 ( 2 ) 85 - 94 2014.2
Language：Japanese Publishing type：Research paper (scientific journal)
DOI： 10.3136/nskkk.61.85
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Takeda S., Fujimoto R., Takenoyama S., Takeshita M., Kikuchi Y., Tsend-Ayush C., Dashnyam B., Muguruma M., Kawahara S.
Food Science and Technology Research 19 ( 2 ) 245 - 253 2013.7
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Food Science and Technology Research
We screened suitable lactic acid bacteria (LAB) strains for use in fermented dairy products by analyzing milk fermented with 10 probiotic LAB strains from Mongolian dairy products. Lactobacillus paracasei paracasei strain 06TCa19 was selected because of its favorable effects on pH, lactic acid production, and viable bacterial numbers after fermentation of skim milk. Then, we prepared 06TCa19 and control fermented milks and conducted a randomized, double-blind crossover study with 46 healthy women to determine the effects of the strain on human defecation. The ingestion of 06TCa19 fermented milk improved the subjects' fecal characteristics, including shape and color. Analysis of stool samples from 8 subjects revealed that l-lactic acid levels and Lactobacillus and Bifidobacterium numbers increased. Moreover, strain 06TCa19 was suggested to reach and survive in the intestines, and is, therefore, suitable for fermented dairy products and can potentially improve human defecation.
DOI： 10.3136/fstr.19.245
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Effects of oral administration of probiotics from Mongolian dairy products on the Th1 immune response in mice Reviewed
Takeda S., Kawahara S., Hidake M., Yoshida H., Watanabe W., Takeshita M., Kikuchi Y., Bumbein D., Muguruma M., Kurokawa M.
Bioscience, Biotechnology, and Biochemistry 77 ( 7 ) 1372 - 1378 2013.7
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.1271/bbb.120624
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Cytokine mediated immune responses in the Japanese pufferfish (Takifugu rubripes) administered with heat-killed Lactobacillus paracasei spp. paracasei (06TCa22) isolated from the Mongolian dairy product Reviewed
Biswas G., Korenaga H., Nagamine R., Kawahara S., Takeda S., Kikuchi Y., Dashnyam B., Yoshida T., Kono T, Sakai M.
International Immunopharmacology 17 ( 2 ) 358 - 365 2013.7
Language：English Publishing type：Research paper (scientific journal)
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Elevated cytokine responses to Vibrio harveyi infection in the Japanese pufferfish (Takifugu rubripes) treated with Lactobacillus paracasei spp. paracasei (06CTa22) isolated from the Mongolian dairy products Reviewed
Biswas G., Korenaga H., Nagamine R., Kawahara S., Takeda S., Kikuchi Y., Dashnyam B., Yoshida T., Kono T, Sakai M.
Fish & Shellfish immunology 35 ( 3 ) 756 - 765 2013.6
Language：English Publishing type：Research paper (scientific journal)
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Cytokine responses in the Japanese pufferfish (Takifugu rubripes) head kidney cells induced with heat-killed probiotics isolated from the Mongolian dairy products Reviewed
Biswas G., Korenaga H., Nagamine R., Takayama H., Kawahara S., Takeda S., Kikuchi Y., Dashnyam B., Kono T, Sakai M.
Fish & Shellfish immunology 34 ( 5 ) 1170 - 1177 2013.5
Language：English Publishing type：Research paper (scientific journal)
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Application of probiotics from Mongolian dairy products to fermented dairy products and its effects on human defecation Reviewed
Takeda S., Fujimoto R., Takenoyama S., Takeshita M., Kikuchi Y., Tsend-Ayush C., Dashnyam B., Muguruma M., Kawahara S.
Food Science and Technology Research 19 ( 2 ) 245 - 253 2013.3
Language：English Publishing type：Research paper (scientific journal)
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Effects of liver hydrolysate on the blood glucose in metabolic syndrome model rats (SHR/NDmcr-cp) Reviewed
Inoue I., Hidaka S., Miura N., Yamada K., Fukahori K., Maruyama M., Kawahara S., Ohta K., Muguruma M.
Yakugaku Zasshi 133 ( 1 ) 117 - 123 2013.1
Language：Japanese Publishing type：Research paper (scientific journal)
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Analysis of the components of porcine liver hydrolysate and examination of the antioxidant activity and angiotensin converting enzyme (ACE) -inhibiting activity Reviewed
Inoue I., Hamasaki A., Hidaka S., Miura N., Fukahori K., Maruyama M., Kawahara S., Ohta K., Muguruma M.
Yakugaku Zasshi 133 ( 1 ) 107 - 115 2013.1
Language：Japanese Publishing type：Research paper (scientific journal)
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Inoue Naonori, Hamasaki Atsuko, Hidaka Shuji, Miura Naoyoshi, Fukahori Masahiro, Maruyama Masugi, Kawahara Satoshi, Ohta Kazuyoshi, Muguruma Michio
YAKUGAKU ZASSHI 133 ( 1 ) 107 - 115 2013
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Pharmaceutical Society of Japan
Hypertension and oxidant stress predispose to the onset and progression of arteriosclerotic diseases. In this study, the components of two kinds of porcine liver hydrolysates (LH-I and LH-II) were analyzed, and the antioxidant effects and angiotensin converting enzyme (ACE)-inhibiting effects of LH-I and LH-II were examined <i>in vitro</i>. Furthermore, the effects of LH-I and LH-II on the blood pressure were examined in spontaneously hypertensive rats (SHR). The results showed that peptides and amino acids accounted for 70% or more of the constituents of both LH-I and LH-II. The results of gel filtration HPLC showed that most of the nitrogen-containing components were peptides or amino acids with molecular weights of 6000 or less. The DPPH radical scavenging activities of LH-I and LH-II were 55.6 and 38.1 μ<small>M</small> Trolox Equivalent/g, respectively. The IC<sub>50</sub> values for the ACE-inhibiting activity of LH-I and LH-II were 0.18 and 0.31 mg/mL, respectively. Oral administration of 1 g/rat of LH-I or LH-II to SHR resulted in significant lowering of the blood pressure. These findings indicate that both LH-I and LH-II have antioxidant activity and ACE-inhibiting activity. Moreover, both exerted a blood pressure-lowering effect in SHR. The antioxidant activity and ACE-inhibiting activity of LH-I and LH-II are presumed to be based on the actions of the component peptides.<br>
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Effects of Liver Hydrolysate on the Blood Glucose in Metabolic Syndrome Model Rats (SHR/NDmcr-cp)
Inoue Naonori, Hidaka Shuji, Miura Naoyoshi, Yamada Kotaro, Fukahori Masahiro, Maruyama Masugi, Kawahara Satoshi, Ohta Kazuyoshi, Muguruma Michio
YAKUGAKU ZASSHI 133 ( 1 ) 117 - 123 2013
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Pharmaceutical Society of Japan
Insulin resistance associated with visceral fat obesity has been suggested to be the pathological basis of metabolic syndrome. Many studies have demonstrated increased oxidant stress in diabetic patients and animal models of diabetes mellitus. In this study, the effect of liver hydrolysate administration on the blood glucose was examined in SHR/NDmcr-cp (SHR-cp) rats that show spontaneously occurring metabolic syndrome-like abnormalities. The SHR-cp rats were fed diets containing 5% liver hydrolysate for 12 weeks, and the fasting blood glucose and HbA1c were determined every 3 weeks. After administration of the liver hydrolysate-containing feed for 12 weeks, an oral glucose tolerance test was conducted and the plasma angiotensin II (AngII) concentrations were determined. The liver hydrolysate administration had no effect on the blood insulin levels in the oral glucose tolerance test, but significantly inhibited the <i>d</i>-glucose-induced increases of the blood glucose levels. Furthermore, the liver hydrolysate had almost no effect on the fasting blood glucose level, but tended to inhibit the increase of HbA1c. The plasma AngII concentration after the 12-week administration of liver hydrolysate remained significantly lower than that in the control group. These results indicate that a component of liver hydrolysate inhibits <i>d</i>-glucose-induced increase of the blood glucose level, and may improve insulin resistance. The angiotensin converting enzyme (ACE)-inhibiting effect and antioxidant effect of liver hydrolysate may be involved in this effect.<br>
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A method for analyzing the purity of un-fractionated heparin preparations and the industrial-scale removal of their impurities Reviewed
Murata H., Matsuhisa A., Yamamoto K., Toda T., Muguruma M., Kawahara S.
Nippon Shokuhin Kagaku Kougaku Kaishi 59 ( 12 ) 628 - 636 2012.12
Language：Japanese Publishing type：Research paper (scientific journal)
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MURATA Hiroshi, MATSUHISA Akio, YAMAMOTO Keiichi, TODA Takao, MUGURUMA Michio, KAWAHARA Satoshi
NIPPON SHOKUHIN KOGYO GAKKAISHI 59 ( 12 ) 628 - 636 2012.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Society for Food Science and Technology
We have developed an analytical method for mucopolysaccharides (MSs) and a simple method for removing their impurities from un-fractionated heparin (UFH) preparations. An over-sulfated chondroitin sulfate standard (OSCS-STD) was de-polymerized with nitrous acid, as were UFH sodium salt containing OSCS and other MSs (OSHP-SH), OSCS reference standards containing heparin (CSMS-CE1 and CSMS-CE2), and various UFH products. Following de-polymerization, the molecular weight distributions of these samples were analyzed by gel permeation HPLC. The molecular weight distribution of OSCS-STD remained unchanged before and after nitrous acid de-polymerization, and the molecular weight distribution of OSCS-STD was similar to that of OSHP-SH de-polymerized with nitrous acid. On the other hand, the molecular weight distributions of CSMS-CE1 and CSMS-CE2 were similar to that of standard chondroitin sulfate B. UFH sodium salt and several drug substances containing heparin and other MSs were then purified by ethanol precipitation. After nitrous acid de-polymerization, gel permeation HPLC and <SUP>1</SUP>H-NMR spectroscopy revealed that MSs resistant to de-polymerization with nitrous acid were concentrated in the supernatant and heparin was concentrated in the colloidal precipitate.
DOI： 10.3136/nskkk.59.628
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A combination of soybean and skimmed milk reduces osteoporosis in rats Reviewed
Muguruma M., Ahhmed A.M., Kawahara S., Kusumegi K., Hishinuma T., Ohya K., Nakamura T.
Journal of Functional Foods 4 ( 4 ) 810 - 818 2012.10
Language：English Publishing type：Research paper (scientific journal)
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Efficacy of oral administration of heat-killed probiotics from Mongolian dairy products against influenza infection in mice: Alleviation of influenza infection by its immunomodulatory activity through intestinal immunity Reviewed
Takeda S., Takeshita M., Kikuchi Y., Dashnyam B., Kawahara S., Yoshida H., Watanabe W., Muguruma M., Kurokawa M.
International Immunopharmacology 11 ( 12 ) 1976 - 1983 2011.12
Language：English Publishing type：Research paper (scientific journal)
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TAKEDA Shiro, YAMASAKI Keiko, TAKESHITA Masahiko, KIKUCHI Yukiharu, TSEND-AYUSH Chuluunbat, DASHNYAM Bumbein, AHHMED Abdulatef M., KAWAHARA Satoshi, MUGURUMA Michio
Animal science journal 82 ( 4 ) 571 - 579 2011.8
Language：Japanese Publishing type：Research paper (scientific journal)
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Expression and postmortem proteolysis of cytoskeletal protein talin in chicken meat Reviewed
Yoshito Tomisaka, Abdulatef M. Ahhmed, Satoshi Kawahara, Michio Muguruma
Journal of Warm Regional Society of Animal Science, Japan 54 ( 1 ) 39 - 48 2011.4
Language：English Publishing type：Research paper (scientific journal)
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Expression and Postmortem Proteolysis of Cytoskeletal Protein Talin in Chicken Meat
TOMISAKA Yoshito, AHHMED Abdulatef M, KAWAHARA Satoshi, MUGURUMA Michio
Nihon Souchi Gakkai Kyushu Shibukaiho 54 ( 1 ) 39 - 48 2011
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Warm Regional Society of Animal Science, Japan
The cytoskeletal protein talin acts as an essential link between integrins and the actin cytoskeleton in costameres. Some studies have reported that talin may contribute to the water-holding capacity of aged meat. Talin has two isoforms, talin1 (T1) and talin2 (T2), but their respective biological roles are not fully understood. Furthermore, the specific roles of T1 and T2 in determining meat quality are unclear. In this study, we analyzed the expression of T1 and T2 in the pectoralis major, biceps femoris, gizzard, and heart in chickens. Additionally, we examined the postmortem changes in talin in the biceps femoris. Expression of T1 mRNA was significantly higher while that of T2 was significantly lower in the gizzard than in the pectoralis major (<I>P</I> < 0.01). Expression of T1 in the gizzard was also signifi cantly higher than in the biceps femoris and heart (<I>P</I> < 0.01). In western blotting using 8d4, anti-talin2 or TA205 antibody, only anti-talin2 could detect talin in the pectoralis major and biceps femoris. Microscopy also showed that talin labeled by anti-talin2, but not 8d4 was located around muscle fibers in the biceps femoris. Furthermore, intact talin band labeled by anti-talin2 within chicken biceps femoris muscle was rapidly diminished during postmortem storage. Our findings propose that the degradation of T2, but not T1, in chicken skeletal muscle may influence the quality of chicken meat.
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Effects of osmotic dehydration sheets on freshness parameters of Thunnus thynnus stored at cold temperatures Reviewed
K. Saito, N. Hamada-Sato, A.M. Ahhmed, S. Kawahara, M. Muguruma
Journal of Food Processing and Preservation 34 1103 - 1120 2010.12
Language：English Publishing type：Research paper (scientific journal)
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Saito K., Hamada-Sato N., Ahhmed A., Ahhmed A., Kawahara S., Muguruma M., Muguruma M.
Journal of Food Processing and Preservation 34 ( 6 ) 1103 - 1120 2010.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of Food Processing and Preservation
In this study, we evaluated the effects of osmotic dehydration sheets (ODSs) on quality parameters and morphological observation of tuna stored at 4C for 4 days. The use of ODSs did not affect Hunter L*and b*values, although use of low performance ODSs (ODS-L) gave higher a*values and lower metmyoglobin percentage (Met-Mb%) than other samples (P < 0.05). As increasingly higher-performance ODSs were used, the total sample content of ATP-related compounds and IMP (a component of umami) increased and the K values decreased (P < 0.05), indicating maintenance of tuna freshness. However, Hunter color values and Met-Mb% revealed that the optimum dehydration level exists at low dehydration performance. ODS was effective in removing intercellular water of thawed tuna tissue. We therefore suggest ODS-L as a functional tool for maintaining the color of tuna, and high performance ODS as a functional tool for maintaining freshness and reform watery texture of chilled stored tuna. © 2009 Wiley Periodicals, Inc.