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Faculty of Medicine School of Medicine Department of Infectious Diseases, Parasitology |
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MARUYAMA Haruhiko
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Papers 【 display / non-display 】
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Ishida M, Irie T, Tanaka R, Maruyama H, Yoshida A
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 135 105839 2025.11
Language：English Publishing type：Research paper (scientific journal) Publisher：Infection Genetics and Evolution
Strongyloides ratti is an intestinal nematode commonly found in rats. Unlike other Strongyloides species, the tissue-migrating third-stage larvae in S. ratti follow a unique route of invasion via the nasofrontal region before reaching the gut. Despite its importance in host invasion, the transcriptomic profile of this larval stage has not been characterized. In this study, we performed RNA sequencing (RNA-seq) to examine gene expression in head-derived tissue-migrating third-stage larvae (hL3) and infective third-stage larvae (iL3) of the S. ratti Tokyo strain. hL3 were collected from rat heads at 30 h post-infection. Differential expression analysis revealed 664 upregulated genes in hL3. Functional annotation showed enrichment of genes encoding astacin metalloproteases and sperm-coating protein/Tpx-1/Ag5/PR-1/Sc7 (SCP/TAPS) protein families—both associated with tissue invasion and immune modulation. Quantitative RT-PCR was used to validate selected differentially expressed genes. Seven hL3-specific astacin genes were identified, of which six belonged to the M12A group. One hL3-specific astacin gene showed domain similarity to strongylastacin, a known tissue-penetration protein. Two SCP/TAPS genes were unique to hL3 and were absent from parasitic females, suggesting distinct roles in larval migration. By contrast, G protein-coupled receptor genes, particularly those related to chemosensory functions, were not upregulated in hL3, indicating that these pathways may be less important during this stage. These results provide the first transcriptomic profile of hL3 in S. ratti, and identify potential molecular mechanisms driving larval migration and immune evasion during host infection.
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A Case of Fasciolosis Reinfection in Japan Reviewed
Akiyoshi K, Kokubo-Tanaka M, Yamauchi S, Yonezu K, Abe I, Tawara K, Saito S, Kondo H, Fukuda T, Fukui A, Akioka H, Shinohara T, Teshima Y, Yufu K, Shimono N, Mizuno Y, Irie T, Yoshida A, Takahashi N, Maruyama H.
Internal medicine 65 ( 6 ) 921 - 925 2025.8
Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Internal Medicine
Fasciolosis is a major cause of food-borne parasitic zoonosis. It primarily affects ruminants but also infects humans. Clinically, it is characterized by abnormal liver images with prominent eosinophilia. We herein report a 77-year-old Japanese livestock farmer, who experienced fasciolosis reinfection. Environmental investigation of his residence revealed that both intermediate host snails and sika deer, a natural final host, inhabited his house. In addition, cattle were infected with Fasciola. Based on these findings, we concluded that his work place was accidentally contaminated with Fasciola metacercariae.
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Down-regulation of colon mucin production induced by Eimeria pragensis infection in mice Reviewed International coauthorship
https://pubmed.ncbi.nlm.nih.gov/40630939/#:~:text=Setia%20YD%2C%20Kokubo%2DTanaka%20M%2C%20Tanaka%20R%2C%20Yoshida%20A%2C%20Nagayasu%20E%2C%20Ahmadi%20P%2C%20Yoshida%20A%2C%20Maruyama%20H.
Frontiers in Parasitology 4 1621486 2025.6
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Frontiers Media S.A.
Introduction: Eimeria pragensis, an intestinal protozoa infecting mice, induces colitis and reduces goblet cell numbers in the large intestine. In the present study, we investigated the pathogenesis and the mechanisms underlying goblet cell down-regulation in the early phase of infection.
Methods: Male C57BL/6 mice were orally infected with 300 oocysts. Fecal oocyst shedding and body weight were monitored daily. Colon tissues were collected at 3, 8, and 13 days post-infection (dpi) to assess pathological changes. Parasite burden was assessed by histological analysis (H&E staining) and qPCR targeting 5S rRNA. Goblet cells were visualized using PAS-Alcian Blue staining and Muc2 immunohistochemistry. To elucidate mechanisms of goblet cell dysfunction, we performed RNA sequencing of large intestine tissue to examine host as well as parasite transcriptomes.
Results: Fecal oocyst excretion peaked at 8–9 dpi. Body weight decreased from 6 to 11 dpi, with recovery after 12 dpi. Maximal parasite accumulation in the proximal colon was observed at 8 dpi in histological examination as well as qPCR. Colon length was significantly shortened at 3 dpi. Goblet cell area significantly reduced at 8 dpi (p < 0.05). RNA sequencing of infected large intestines revealed that E. pragensis produced enzymes that were known to degrade mucin and tight junctions, and proteins that could activate the Notch–Hes1 signaling pathway. As for host responses, genes associated with Th1-type inflammation, epithelial barrier disruption, and immune regulation were up-regulated as early as 3 dpi.
Discussion: Our findings suggested that E. pragensis infection induces a mucosal barrier dysfunction in the early phase of the infection, which possibly causes the tissue invasion of bacteria in the large intestine. Th1-type inflammatory response, thus induced, reduces goblet cell numbers and mucin production. This model provides valuable insight into the mechanisms of mucosal barrier disruption during protozoan infection. -
Four successive cases of human fasciolosis in Japan Reviewed
Ueda Michimasa, Katayama Atsushi, Matsuda Risa, Kumabe Ayako, Ichikawa-Seki Madoka, Harada Takanori, Yoshida Ayako, Kaneko Chiho, Doi Asako, Kitaura Tsuyoshi, Maruyama Haruhiko, Chikumi Hiroki
Journal of Infection and Chemotherapy 31 ( 2 ) 102480 2025.2
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
Fasciolosis is a food-borne parasitic disease, caused by the large liver fluke, Fasciola. Humans acquire infection by ingesting fresh or undercooked water plants, on which infective metacercaria encyst. In spite of the rarity of the disease in Japan, we encountered four successive fasciolosis patients within a short period, who were all living in the same area. The patients were 70–82 years old, three females and the husband of one of the female patients. They started complaining of non-specific symptoms, such as fever, general fatigue, appetite loss, and abdominal pain, almost at the same time. All patients showed prominent peripheral blood eosinophilia, and the medical imaging indicated multiple hepatic lesions. No parasite eggs or worms were detected in any of the patients. Diagnosis was made serologically and they were treated with praziquantel and/or triclabendazole. No cattle or sheep were farmed in the area, but the wild sika deer, Cervus nippon, inhabited adjacent to the residential area. The intermediate host snail, Austropeplea ollula, were found near the residence of the patients, and one of the collected snails was positive for F. hepatica/gigantica hybrid type rediae. Our report should alarm the medical professionals for this rare and unfamiliar parasitic disease.
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Detection and analysis of Serpin and RP26 specific antibodies for monitoring Schistosoma haematobium transmission. Reviewed International coauthorship
Kokubo-Tanaka M, Kildemoes AO, Chadeka EA, Cheruiyot BN, Moriyasu T, Sassa M, Nakamura R, Kikuchi M, Fujii Y, de Dood CJ, Corstjens PLAM, Kaneko S, Maruyama H, Njenga SM, de Vrueh R, Hokke CH, Hamano S
PLoS neglected tropical diseases 19 ( 1 ) e0012813 2025.1
Language：English Publishing type：Research paper (scientific journal) Publisher：PLoS Neglected Tropical Diseases
Background Schistosoma haematobium is the causative pathogen for urogenital schistosomiasis. To achieve progress towards schistosomiasis elimination, there is a critical need for developing highly sensitive and specific tools to monitor transmission in near-elimination settings. Although antibody detection is a promising approach, it is usually unable to discriminate active infections from past ones. Moreover, crude antigens such as soluble egg antigen (SEA) show cross-reactivity with other parasitic infections, and it is difficult to formulate the standard preparations. To resolve these issues, the performances of recombinant antigens have been evaluated. The antibody responses against recombinant S. haematobium ser-ine-protease inhibitor (ShSerpin) and RP26 were previously shown to reflect active schisto-some infection in humans. Furthermore, antibody detection using multiple recombinant antigens has been reported to improve the accuracy of antibody-based assays compared to single-target assays. Therefore, we examined the performances of ShSerpin, RP26 and the mixture of these antigens for detecting S. haematobium low-intensity infection and assessed the potential for transmission monitoring. Methodology/Principal findings We collected urine and plasma samples from school-aged children in Kwale, Kenya and evaluated S. haematobium prevalence by number of eggs in urine and worm-derived circulating anodic antigen (CAA) in plasma. Among 269 pupils, 50.2% were CAA-positive by the lateral flow test utilizing up-converting phosphor particles (UCP-LF CAA), while only 14.1% were egg-positive. IgG levels to S. haematobium SEA (ShSEA), ShSerpin, RP26, and the mixture of ShSerpin and RP26 were measured by ELISA. The mixture of ShSerpin and RP26 showed the highest sensitivity, 88.7%(125/141)among the four antigens in consider-ing indecisive UCP-LF CAA results as negative. Conclusion/Significance IgG detection against the ShSerpin-RP26 mixture demonstrated better sensitivity for detection of active S. haematobium infection. This recombinant antigen mixture is simpler to pro-duce with higher reproducibility and can potentially replace ShSEA in monitoring transmission under near-elimination settings.
Books 【 display / non-display 】
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臨床検査法提要 改訂第35版
丸山 治彦（ Role： Joint author , Ⅲ．寄生虫・原虫検査、Ⅳ．衛生動物）
金原出版株式会社 2020.5
Responsible for pages：1269-1300 Language：Japanese Book type：Dictionary, encyclopedia
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今日の診断指針 第8版
丸山 治彦（ Role： Joint editor , 糞線虫症）
医学書院 2020.3
Responsible for pages：1362-1364 Language：Japanese Book type：Dictionary, encyclopedia
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治療薬UP-TO-DATE 2020
丸山 治彦（ Role： Joint author）
メディカルレビュー社 2020.1
Language：Japanese Book type：Dictionary, encyclopedia
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治療薬ハンドブック 2020
丸山 治彦（ Role： Joint author）
じほう 2020.1
Language：Japanese Book type：Dictionary, encyclopedia
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今日の治療方針 2020
丸山 治彦（ Role： Joint editor）
医学書院 2020.1
Language：Japanese Book type：Dictionary, encyclopedia
MISC 【 display / non-display 】
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Identification of a bacteria-like ferrochelatase in Strongyloides venezuelensis, an animal parasitic nematode. Reviewed
Nagayasu E, Ishikawa SA, Taketani S, Chakraborty G, Yoshida A, Inagaki Y, Maruyama H.
PLoS One 8 ( 3 ) e58458 2013.3
Authorship：Last author, Corresponding author Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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Strongyloidiasis in recently arrived captive-bred meerkats imported to Japan
Nagayasu E., Takaki Y., Takami Y., Yoshida A., Une Y., Maruyama H.
Journal of Exotic Pet Medicine 40 10 - 11 2022.1
Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：Journal of Exotic Pet Medicine
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肝蛭症の1例 肝好酸球性膿瘍を来す稀な寄生虫感染症 Reviewed
樺澤 崇允, 山谷 英之, 丸山 治彦, 浦野 友佳, 鈴木 一司, 北岡 匠, 玉澤 暢之, 宇都宮 文, 大江 倫太郎, 山川 光徳
診断病理 38 ( 1 ) 40 - 43 2021.1
Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：(一社)日本病理学会
中南米在住の20代の女性。日本に帰国し、難治性の肝膿瘍を切除された。病理組織学的には寄生虫感染による好酸球性膿瘍であり、免疫検査や遺伝子検査の結果を総合して肝蛭症と診断した。肝蛭症は本邦では稀な感染症であり、肝好酸球性膿瘍を形成することがある。病理組織標本で虫卵を伴った肝好酸球性膿瘍がみられた場合には、鑑別すべき疾患は肝蛭症か回虫移行症に限定される。虫体が検出できない場合は免疫検査や遺伝子検査を併用することで肝蛭症の最終診断に至ることが可能である。(著者抄録)
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治療法の再整理とアップデートのために 専門家による私の治療 顎口虫症 Invited
丸山 治彦
日本医事新報 ( 5033 ) 40 - 41 2020.10
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：(株)日本医事新報社
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感染症の今日的問題点 寄生虫症からかいま見える現代日本社会 Invited
丸山 治彦
感染症学雑誌 94 ( 臨増 ) 106 - 106 2020.3
Authorship：Lead author Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：(一社)日本感染症学会
Presentations 【 display / non-display 】
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わが国における食肉由来寄生虫症 Invited
丸山治彦
第160回日本獣医学会集会 食肉の安全性に関する市民公開シンポジウム 「食肉に由来する感染症とその予防」
Event date： 2017.9.13 - 2017.9.15
Language：Japanese Presentation type：Oral presentation (invited, special)
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わが国における食肉由来寄生虫症 International conference
丸山治彦
第160回日本獣医学会集会 食肉の安全性に関する市民公開シンポジウム 「食肉に由来する感染症とその予防」
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血清疫学的手法による鶏肉・内臓を介したヒトの動物由来回虫症への感染リスク評価
吉田彩子, Yen Thi HoangNguyen, 丸山治彦, 堀井洋一郎, 野中成晃
第159回日本獣医学会学術集会
Event date： 2016.9.6 - 2016.9.8
Language：Japanese Presentation type：Oral presentation (general)
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血清疫学的手法による鶏肉・内臓を介したヒトの動物由来回虫症への感染リスク評価 International conference
吉田彩子, Yen Thi HoangNguyen, 丸山治彦, 堀井洋一郎, 野中成晃
第159回日本獣医学会学術集会
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ウエステルマン肺吸虫症の原因食品としてのシカ肉の可能性
吉田彩子, 松尾加代子, 長安英治, 丸山治彦
第26回日本臨床寄生虫学会大会
Event date： 2016.6.20
Language：Japanese Presentation type：Oral presentation (general)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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芽殖孤虫症様マンソン孤虫症のゲノムリシーケンスと発現遺伝子解析による病態解明
Grant number：24K10192 2024.04 - 2027.03
独立行政法人日本学術振興会 科学研究費基金 基盤研究(C)
Authorship：Principal investigator
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磁性ナノ粒子を用いた蠕虫のin vivoイメージングによる体内移行動態の解析
Grant number：21K06995 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
吉田 彩子,
Authorship：Coinvestigator(s)
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寄生虫の分子同定と系統解析のための統一標準法の確立
Grant number：21H02725 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
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実臨床データを利用した寄生虫症最適検査診断システムの構築
Grant number：18K07090 2018 - 2021.03
科学研究費補助金 基盤研究(C)
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芽殖孤虫のゲノム解読による条虫類幼虫における無性増殖機構の解明
Grant number：26460510 2014.04 - 2017.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
芽殖孤虫のゲノム解読による条虫類幼虫における無性増殖機構の解明
Other research activities 【 display / non-display 】
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ウガンダ
2013.05
国際協同研究打合せ（Gulu大学）
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寄生虫症薬物治療の手引き2010
2009.11 - 2010.04
熱帯病治療薬研究班の知見をまとめた、熱帯病および寄生虫疾患の治療ガイドラインの編集。わが国においては寄生虫病や熱帯病に対する薬剤は国内未承認あるいは保険適用外であることが多く、ガイドラインの編集作製が急務であったことに対応したもの。
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寄生虫症の血清疫学
2006.10
日本各地の医療機関からの寄生虫病血清診断依頼に対応しわが国の寄生虫病相を明らかにする
Available Technology 【 display / non-display 】
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Related fields where technical consultation is available：次世代DNAシーケンサを用いた病原微生物の塩基配列解析
Message：寄生虫症は数は少ないながら日本から消滅することはありません。また海外には莫大な数の感染者がおります。グローバルヘルスを考えた場合には、とても大事な研究分野なのです。
