MARUYAMA Haruhiko

写真a

Affiliation

Faculty of Medicine School of Medicine Department of Infectious Diseases, Parasitology

Title

Professor

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Degree 【 display / non-display

  • 医学博士 ( 1990.3   宮崎医科大学 )

Research Areas 【 display / non-display

  • Life Science / Parasitology

 

Papers 【 display / non-display

  • Four successive cases of human fasciolosis in Japan. Reviewed

    Kumabe A, Doi A, Kitaura T, Katayama A, Harada T, Ueda M, Matsuda R, Ichikawa-Seki M, Tanaka M, Kaneko C, Yoshida A, Chikumi H, Maruyama H

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   31 ( 2 )   102480   2025.2

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Infection and Chemotherapy  

    Fasciolosis is a food-borne parasitic disease, caused by the large liver fluke, Fasciola. Humans acquire infection by ingesting fresh or undercooked water plants, on which infective metacercaria encyst. In spite of the rarity of the disease in Japan, we encountered four successive fasciolosis patients within a short period, who were all living in the same area. The patients were 70–82 years old, three females and the husband of one of the female patients. They started complaining of non-specific symptoms, such as fever, general fatigue, appetite loss, and abdominal pain, almost at the same time. All patients showed prominent peripheral blood eosinophilia, and the medical imaging indicated multiple hepatic lesions. No parasite eggs or worms were detected in any of the patients. Diagnosis was made serologically and they were treated with praziquantel and/or triclabendazole. No cattle or sheep were farmed in the area, but the wild sika deer, Cervus nippon, inhabited adjacent to the residential area. The intermediate host snail, Austropeplea ollula, were found near the residence of the patients, and one of the collected snails was positive for F. hepatica/gigantica hybrid type rediae. Our report should alarm the medical professionals for this rare and unfamiliar parasitic disease.

    DOI: 10.1016/j.jiac.2024.07.020

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  • Detection and analysis of Serpin and RP26 specific antibodies for monitoring Schistosoma haematobium transmission. Reviewed International coauthorship

    Kokubo-Tanaka M, Kildemoes AO, Chadeka EA, Cheruiyot BN, Moriyasu T, Sassa M, Nakamura R, Kikuchi M, Fujii Y, de Dood CJ, Corstjens PLAM, Kaneko S, Maruyama H, Njenga SM, de Vrueh R, Hokke CH, Hamano S

    PLoS neglected tropical diseases   19 ( 1 )   e0012813   2025.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PLoS Neglected Tropical Diseases  

    Background Schistosoma haematobium is the causative pathogen for urogenital schistosomiasis. To achieve progress towards schistosomiasis elimination, there is a critical need for developing highly sensitive and specific tools to monitor transmission in near-elimination settings. Although antibody detection is a promising approach, it is usually unable to discriminate active infections from past ones. Moreover, crude antigens such as soluble egg antigen (SEA) show cross-reactivity with other parasitic infections, and it is difficult to formulate the standard preparations. To resolve these issues, the performances of recombinant antigens have been evaluated. The antibody responses against recombinant S. haematobium ser-ine-protease inhibitor (ShSerpin) and RP26 were previously shown to reflect active schisto-some infection in humans. Furthermore, antibody detection using multiple recombinant antigens has been reported to improve the accuracy of antibody-based assays compared to single-target assays. Therefore, we examined the performances of ShSerpin, RP26 and the mixture of these antigens for detecting S. haematobium low-intensity infection and assessed the potential for transmission monitoring. Methodology/Principal findings We collected urine and plasma samples from school-aged children in Kwale, Kenya and evaluated S. haematobium prevalence by number of eggs in urine and worm-derived circulating anodic antigen (CAA) in plasma. Among 269 pupils, 50.2% were CAA-positive by the lateral flow test utilizing up-converting phosphor particles (UCP-LF CAA), while only 14.1% were egg-positive. IgG levels to S. haematobium SEA (ShSEA), ShSerpin, RP26, and the mixture of ShSerpin and RP26 were measured by ELISA. The mixture of ShSerpin and RP26 showed the highest sensitivity, 88.7%(125/141)among the four antigens in consider-ing indecisive UCP-LF CAA results as negative. Conclusion/Significance IgG detection against the ShSerpin-RP26 mixture demonstrated better sensitivity for detection of active S. haematobium infection. This recombinant antigen mixture is simpler to pro-duce with higher reproducibility and can potentially replace ShSEA in monitoring transmission under near-elimination settings.

    DOI: 10.1371/journal.pntd.0012813

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  • Syntenic relationship of chromosomes in Strongyloides species and Rhabditophanes diutinus based on the chromosome-level genome assemblies Reviewed International coauthorship

    Kounosu Asuka, Sun Simo, Maeda Yasunobu, Dayi Mehmet, Yoshida Akemi, Maruyama Haruhiko, Hunt Vicky, Sugimoto Asako, Kikuchi Taisei

    Philosophical Transactions of the Royal Society B: Biological Sciences   379 ( 1894 )   20220446   2024.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    The Strongyloides clade, to which the parasitic nematode genus Strongyloides belongs, contains taxa with diverse lifestyles, ranging from free-living to obligate vertebrate parasites. Reproductive strategies are also diverse in this group of nematodes, employing not only sexual reproduction but also parthenogenesis, making it an attractive group to study genome adaptation to specific conditions. An in-depth understanding of genome evolution, however, has been hampered by fragmented genome assemblies. In this study, we generated chromosome-level genome assemblies for two Strongyloides species and the outgroup species Rhabditophanes diutinus using long-read sequencing and high‐throughput chromosome conformation capture (Hi-C). Our synteny analyses revealed a clearer picture of chromosome evolution in this group, suggesting that a functional sex chromosome has been maintained throughout the group. We further investigated sex chromosome dynamics in the lifecycle of Strongyloides ratti and found that bivalent formation in oocytes appears to be important for male production in the mitotic parthenogenesis.

    This article is part of the Theo Murphy meeting issue ‘Strongyloides: omics to worm-free populations’.

    DOI: 10.1098/rstb.2022.0446

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  • Primaquine plus clindamycin as a promising salvage therapy for Pneumocystis jirovecii pneumonia: A retrospective analysis in Japanese patients Reviewed

    Michiko Koga, Akihiko Suganuma, Tadashi Kikuchi, Yukihiro Yoshimura, Kensuke Shoji, Ichiro Kobayashi, Shunichiro Takezaki, Yasuyuki Kato, Mikio Kimura, Haruhiko Maruyama

    Journal of Infection and Chemotherapy   27 ( 6 )   924 - 928   2021.6

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.jiac.2021.03.001

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  • Enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola cathepsin L1 for the diagnosis of human fasciolosis caused by Fasciola hepatica/gigantica hybrid type. Reviewed

    Sugiyama T, Ichikawa-Seki M, Sato H, Kounosu A, Tanaka M, Maruyama H

    Parasitology international   82   102311   2021.6

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Parasitology International  

    Recombinant Fasciola cathepsin L-1 (rCatL1) was evaluated in enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of human fasciolosis in Japan. Quality characteristics of the test were accessed by receiver operating characteristic (ROC) analysis, with sera from fasciolosis patients (n = 10), patients with no evidence of parasitic infections (n = 29), and patients with other helminth infections (n = 119). Both the sensitivity and specificity of the test achieved 100% with the control samples. To test the performance of the assay in an authentic situation, 311 serum samples, which had been sent to our laboratory for the diagnosis of parasitic infections from January 2018 to February 2019, were re-assessed using the rCatL1 ELISA. In this case, the sensitivity of the rCatL1 ELISA was 100%, giving positive results to all fasciolosis sera (n = 7), and the specificity was 99.0%, in which three of the 304 non-fasciolosis samples were judged positive. Careful re-examination of the laboratory data and medical imaging of these three patients revealed that one of the patients, who had been diagnosed as having larva migrans syndrome, was judged to be infected with Fasciola, in addition to ascarid nematodes. Thus the true specificity of the assay in the authentic reached 99.3% (302/304). As the rCatL1 ELISA exhibited a highly significant positive likelihood ratio (152.0) and negative likelihood ratio (0.0), calculated from the 311 sample data, this rCatL1 ELISA can be used for routine screening and definitive diagnosis test for fasciolosis in reference laboratories.

    DOI: 10.1016/j.parint.2021.102311

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Books 【 display / non-display

  • 臨床検査法提要 改訂第35版

    丸山 治彦( Role: Joint author ,  Ⅲ.寄生虫・原虫検査、Ⅳ.衛生動物)

    金原出版株式会社  2020.5 

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    Responsible for pages:1269-1300   Language:Japanese Book type:Dictionary, encyclopedia

  • 今日の診断指針 第8版

    丸山 治彦( Role: Joint editor ,  糞線虫症)

    医学書院  2020.3 

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    Responsible for pages:1362-1364   Language:Japanese Book type:Dictionary, encyclopedia

  • 治療薬UP-TO-DATE 2020

    丸山 治彦( Role: Joint author)

    メディカルレビュー社  2020.1 

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    Language:Japanese Book type:Dictionary, encyclopedia

  • 治療薬ハンドブック 2020

    丸山 治彦( Role: Joint author)

    じほう  2020.1 

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    Language:Japanese Book type:Dictionary, encyclopedia

  • 今日の治療方針 2020

    丸山 治彦( Role: Joint editor)

    医学書院  2020.1 

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    Language:Japanese Book type:Dictionary, encyclopedia

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MISC 【 display / non-display

  • Identification of a bacteria-like ferrochelatase in Strongyloides venezuelensis, an animal parasitic nematode. Reviewed

    Nagayasu E, Ishikawa SA, Taketani S, Chakraborty G, Yoshida A, Inagaki Y, Maruyama H.

    PLoS One   8 ( 3 )   e58458   2013.3

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: 10.1371/journal.pone.0058458.

  • Strongyloidiasis in recently arrived captive-bred meerkats imported to Japan

    Nagayasu E., Takaki Y., Takami Y., Yoshida A., Une Y., Maruyama H.

    Journal of Exotic Pet Medicine   40   10 - 11   2022.1

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    Language:Japanese   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)   Publisher:Journal of Exotic Pet Medicine  

    DOI: 10.1053/j.jepm.2021.10.002

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  • 肝蛭症の1例 肝好酸球性膿瘍を来す稀な寄生虫感染症 Reviewed

    樺澤 崇允, 山谷 英之, 丸山 治彦, 浦野 友佳, 鈴木 一司, 北岡 匠, 玉澤 暢之, 宇都宮 文, 大江 倫太郎, 山川 光徳

    診断病理   38 ( 1 )   40 - 43   2021.1

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    Language:Japanese   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)   Publisher:(一社)日本病理学会  

    中南米在住の20代の女性。日本に帰国し、難治性の肝膿瘍を切除された。病理組織学的には寄生虫感染による好酸球性膿瘍であり、免疫検査や遺伝子検査の結果を総合して肝蛭症と診断した。肝蛭症は本邦では稀な感染症であり、肝好酸球性膿瘍を形成することがある。病理組織標本で虫卵を伴った肝好酸球性膿瘍がみられた場合には、鑑別すべき疾患は肝蛭症か回虫移行症に限定される。虫体が検出できない場合は免疫検査や遺伝子検査を併用することで肝蛭症の最終診断に至ることが可能である。(著者抄録)

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  • 治療法の再整理とアップデートのために 専門家による私の治療 顎口虫症 Invited

    丸山 治彦

    日本医事新報   ( 5033 )   40 - 41   2020.10

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)   Publisher:(株)日本医事新報社  

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  • 感染症の今日的問題点 寄生虫症からかいま見える現代日本社会 Invited

    丸山 治彦

    感染症学雑誌   94 ( 臨増 )   106 - 106   2020.3

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    Authorship:Lead author   Language:Japanese   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)   Publisher:(一社)日本感染症学会  

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Presentations 【 display / non-display

  • わが国における食肉由来寄生虫症 Invited

    丸山治彦

    第160回日本獣医学会集会 食肉の安全性に関する市民公開シンポジウム 「食肉に由来する感染症とその予防」 

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    Event date: 2017.9.13 - 2017.9.15

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  • わが国における食肉由来寄生虫症 International conference

    丸山治彦

    第160回日本獣医学会集会 食肉の安全性に関する市民公開シンポジウム 「食肉に由来する感染症とその予防」 

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    Event date: 2017.9.13

    Language:Japanese  

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  • 血清疫学的手法による鶏肉・内臓を介したヒトの動物由来回虫症への感染リスク評価

    吉田彩子, Yen Thi HoangNguyen, 丸山治彦, 堀井洋一郎, 野中成晃

    第159回日本獣医学会学術集会 

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    Event date: 2016.9.6 - 2016.9.8

    Language:Japanese   Presentation type:Oral presentation (general)  

  • 血清疫学的手法による鶏肉・内臓を介したヒトの動物由来回虫症への感染リスク評価 International conference

    吉田彩子, Yen Thi HoangNguyen, 丸山治彦, 堀井洋一郎, 野中成晃

    第159回日本獣医学会学術集会 

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    Event date: 2016.9.6

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  • ウエステルマン肺吸虫症の原因食品としてのシカ肉の可能性

    吉田彩子, 松尾加代子, 長安英治, 丸山治彦

    第26回日本臨床寄生虫学会大会 

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    Event date: 2016.6.20

    Language:Japanese   Presentation type:Oral presentation (general)  

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Grant-in-Aid for Scientific Research 【 display / non-display

  • 芽殖孤虫症様マンソン孤虫症のゲノムリシーケンスと発現遺伝子解析による病態解明

    Grant number:24K10192  2024.04 - 2027.03

    独立行政法人日本学術振興会  科学研究費基金  基盤研究(C)

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    Authorship:Principal investigator 

  • 磁性ナノ粒子を用いた蠕虫のin vivoイメージングによる体内移行動態の解析

    Grant number:21K06995  2021.04 - 2024.03

    独立行政法人日本学術振興会  科学研究費補助金  基盤研究(C)

    吉田 彩子,

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    Authorship:Coinvestigator(s) 

  • 寄生虫の分子同定と系統解析のための統一標準法の確立

    Grant number:21H02725  2021.04 - 2024.03

    独立行政法人日本学術振興会  科学研究費補助金  基盤研究(B)

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    Authorship:Principal investigator 

  • 実臨床データを利用した寄生虫症最適検査診断システムの構築

    Grant number:18K07090  2018 - 2021.03

    科学研究費補助金  基盤研究(C)

  • 芽殖孤虫のゲノム解読による条虫類幼虫における無性増殖機構の解明

    Grant number:26460510  2014.04 - 2017.03

    科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

    芽殖孤虫のゲノム解読による条虫類幼虫における無性増殖機構の解明

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Other research activities 【 display / non-display

  • ウガンダ

    2013.05

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    国際協同研究打合せ(Gulu大学)

  • 寄生虫症薬物治療の手引き2010

    2009.11 - 2010.04

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    熱帯病治療薬研究班の知見をまとめた、熱帯病および寄生虫疾患の治療ガイドラインの編集。わが国においては寄生虫病や熱帯病に対する薬剤は国内未承認あるいは保険適用外であることが多く、ガイドラインの編集作製が急務であったことに対応したもの。

  • 寄生虫症の血清疫学

    2006.10

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    日本各地の医療機関からの寄生虫病血清診断依頼に対応しわが国の寄生虫病相を明らかにする

Available Technology 【 display / non-display

  • 真核生物の網羅的検出法の開発
    国内寄生虫症の抗体検査系開発

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    Related fields where technical consultation is available:次世代DNAシーケンサを用いた病原微生物の塩基配列解析

    Message:寄生虫症は数は少ないながら日本から消滅することはありません。また海外には莫大な数の感染者がおります。グローバルヘルスを考えた場合には、とても大事な研究分野なのです。