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Affiliation |
Faculty of Medicine School of Medicine Department of Infectious Diseases, Parasitology |
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Professor |
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External Link |
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MARUYAMA Haruhiko
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Papers 【 display / non-display 】
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Michiko Koga, Akihiko Suganuma, Tadashi Kikuchi, Yukihiro Yoshimura, Kensuke Shoji, Ichiro Kobayashi, Shunichiro Takezaki, Yasuyuki Kato, Mikio Kimura, Haruhiko Maruyama
Journal of Infection and Chemotherapy 27 ( 6 ) 924 - 928 2021.6
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV
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Sugiyama T, Ichikawa-Seki M, Sato H, Kounosu A, Tanaka M, Maruyama H
Parasitology international 82 102311 2021.6
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Parasitology International
Recombinant Fasciola cathepsin L-1 (rCatL1) was evaluated in enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of human fasciolosis in Japan. Quality characteristics of the test were accessed by receiver operating characteristic (ROC) analysis, with sera from fasciolosis patients (n = 10), patients with no evidence of parasitic infections (n = 29), and patients with other helminth infections (n = 119). Both the sensitivity and specificity of the test achieved 100% with the control samples. To test the performance of the assay in an authentic situation, 311 serum samples, which had been sent to our laboratory for the diagnosis of parasitic infections from January 2018 to February 2019, were re-assessed using the rCatL1 ELISA. In this case, the sensitivity of the rCatL1 ELISA was 100%, giving positive results to all fasciolosis sera (n = 7), and the specificity was 99.0%, in which three of the 304 non-fasciolosis samples were judged positive. Careful re-examination of the laboratory data and medical imaging of these three patients revealed that one of the patients, who had been diagnosed as having larva migrans syndrome, was judged to be infected with Fasciola, in addition to ascarid nematodes. Thus the true specificity of the assay in the authentic reached 99.3% (302/304). As the rCatL1 ELISA exhibited a highly significant positive likelihood ratio (152.0) and negative likelihood ratio (0.0), calculated from the 311 sample data, this rCatL1 ELISA can be used for routine screening and definitive diagnosis test for fasciolosis in reference laboratories.
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Genome of the fatal tapeworm Sparganum proliferum uncovers mechanisms for cryptic life cycle and aberrant larval proliferation. Reviewed International coauthorship International journal
Taisei Kikuchi, Mehmet Dayi, Vicky L Hunt, Kenji Ishiwata, Atsushi Toyoda, Asuka Kounosu, Simo Sun, Yasunobu Maeda, Yoko Kondo, Belkisyole Alarcon de Noya, Oscar Noya, Somei Kojima, Toshiaki Kuramochi, Haruhiko Maruyama
Communications biology 4 ( 1 ) 649 - 649 2021.5
Authorship：Last author Language：English Publishing type：Research paper (scientific journal)
The cryptic parasite Sparganum proliferum proliferates in humans and invades tissues and organs. Only scattered cases have been reported, but S. proliferum infection is always fatal. However, S. proliferum's phylogeny and life cycle remain enigmatic. To investigate the phylogenetic relationships between S. proliferum and other cestode species, and to examine the mechanisms underlying pathogenicity, we sequenced the entire genomes of S. proliferum and a closely related non-life-threatening tapeworm Spirometra erinaceieuropaei. Additionally, we performed larvae transcriptome analyses of S. proliferum plerocercoid to identify genes involved in asexual reproduction in the host. The genome sequences confirmed that the S. proliferum has experienced a clearly distinct evolutionary history from S. erinaceieuropaei. Moreover, we found that nonordinal extracellular matrix coordination allows asexual reproduction in the host, and loss of sexual maturity in S. proliferum are responsible for its fatal pathogenicity to humans. Our high-quality reference genome sequences should be valuable for future studies of pseudophyllidean tapeworm biology and parasitism.
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Nakagawa Y, Ikematsu Y, Nakanishi T, Ogawa Y, Taen R, Nakashima Y, Okabe H, Yoshida A, Maruyama H
Parasitology international 81 102279 2021.4
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Parasitology International
We encountered an outbreak of paragonimiasis among Cambodian technical intern trainees (TITs) at a food-processing factory in Fukuoka, Japan. The patients were 20–28 years old, seven females and two males, who had been in Japan for one to four years. All of them had consumed raw or undercooked Japanese mitten crab they purchased at a local grocery store near their training place. CT images showed multiple lesions not only in the lungs but in the extrapulmonary organs as well, such as subcutaneous tissues, abdominal muscles, and mesentery, in most of the patients. Their medical records indicated that all of them acquired infection in Japan, not in Cambodia. Diagnosis was made serologically and the patients were treated with praziquantel successfully. Foreign workers and TITs are increasing in Japan so rapidly, that food borne-infections, including paragonimiasis, should be considered in people from developing countries who have exotic dietary habits.
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Meningitis patients with Angiostrongylus cantonensis may present without eosinophilia in the cerebrospinal fluid in northern Vietnam. Reviewed International coauthorship International journal
Tomoko Hiraoka, Ngo Chi Cuong, Sugihiro Hamaguchi, Mihoko Kikuchi, Shungo Katoh, Le Kim Anh, Nguyen Thi Hien Anh, Dang Duc Anh, Chris Smith, Haruhiko Maruyama, Lay-Myint Yoshida, Do Duy Cuong, Pham Thanh Thuy, Koya Ariyoshi
PLoS neglected tropical diseases 14 ( 12 ) e0008937 - 15 2020.12
Language：English Publishing type：Research paper (scientific journal)
BACKGROUND: Eosinophilic meningitis (EM) is a rare clinical syndrome caused by both infectious and noninfectious diseases. In tropical pacific countries, Angiostrongylus cantonensis is the most common cause. However, the EM definition varies in the literature, and its relation to parasitic meningitis (PM) remains unclear. METHODOLOGY/PRINCIPAL FINDINGS: Adult and adolescent patients of 13 years old or above with suspected central nervous system (CNS) infections with abnormal CSF findings were prospectively enrolled at a tertiary referral hospital in Hanoi, Vietnam from June 2012 to May 2014. Patients with EM or suspected PM (EM/PM) were defined by the presence of either ≥10% eosinophils or an absolute eosinophil cell counts of ≥10/mm3 in the CSF or blood eosinophilia (>16% of WBCs) without CSF eosinophils. In total 679 patients were enrolled: 7 (1.03%) had ≥10% CSF eosinophilia, 20 (2.95%) had ≥10/mm3 CSF eosinophilia, and 7 (1.03%) had >16% blood eosinophilia. The patients with ≥10% CSF eosinophilia were significantly younger (p = 0.017), had a lower body temperature (p = 0.036) than patients with ≥10/mm3 CSF eosinophilia among whom bacterial pathogens were detected in 72.2% (13/18) of those who were tested by culture and/or PCR. In contrast, the characteristics of the patients with >16% blood eosinophilia resembled those of patients with ≥10% CSF eosinophilia. We further conducted serological tests and real-time PCR to identify A. cantonensis. Serology or real-time PCR was positive in 3 (42.8%) patients with ≥10% CSF eosinophilia and 6 (85.7%) patients with >16% blood eosinophilia without CSF eosinophils but none of patients with ≥10/mm3 CSF eosinophilia. CONCLUSIONS: The etiology of PM in northern Vietnam is A. cantonensis. The eosinophil percentage is a more reliable predictor of parasitic EM than absolute eosinophil count in the CSF. Patients with PM may present with a high percentage of eosinophils in the peripheral blood but not in the CSF.
Books 【 display / non-display 】
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臨床検査法提要 改訂第35版
丸山 治彦（ Role： Joint author , Ⅲ．寄生虫・原虫検査、Ⅳ．衛生動物）
金原出版株式会社 2020.5
Responsible for pages：1269-1300 Language：Japanese Book type：Dictionary, encyclopedia
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今日の診断指針 第8版
丸山 治彦（ Role： Joint editor , 糞線虫症）
医学書院 2020.3
Responsible for pages：1362-1364 Language：Japanese Book type：Dictionary, encyclopedia
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治療薬UP-TO-DATE 2020
丸山 治彦（ Role： Joint author）
メディカルレビュー社 2020.1
Language：Japanese Book type：Dictionary, encyclopedia
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治療薬ハンドブック 2020
丸山 治彦（ Role： Joint author）
じほう 2020.1
Language：Japanese Book type：Dictionary, encyclopedia
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今日の治療方針 2020
丸山 治彦（ Role： Joint editor）
医学書院 2020.1
Language：Japanese Book type：Dictionary, encyclopedia
MISC 【 display / non-display 】
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Strongyloidiasis in recently arrived captive-bred meerkats imported to Japan
Nagayasu E., Takaki Y., Takami Y., Yoshida A., Une Y., Maruyama H.
Journal of Exotic Pet Medicine 40 10 - 11 2022.1
Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：Journal of Exotic Pet Medicine
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肝蛭症の1例 肝好酸球性膿瘍を来す稀な寄生虫感染症 Reviewed
樺澤 崇允, 山谷 英之, 丸山 治彦, 浦野 友佳, 鈴木 一司, 北岡 匠, 玉澤 暢之, 宇都宮 文, 大江 倫太郎, 山川 光徳
診断病理 38 ( 1 ) 40 - 43 2021.1
Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：(一社)日本病理学会
中南米在住の20代の女性。日本に帰国し、難治性の肝膿瘍を切除された。病理組織学的には寄生虫感染による好酸球性膿瘍であり、免疫検査や遺伝子検査の結果を総合して肝蛭症と診断した。肝蛭症は本邦では稀な感染症であり、肝好酸球性膿瘍を形成することがある。病理組織標本で虫卵を伴った肝好酸球性膿瘍がみられた場合には、鑑別すべき疾患は肝蛭症か回虫移行症に限定される。虫体が検出できない場合は免疫検査や遺伝子検査を併用することで肝蛭症の最終診断に至ることが可能である。(著者抄録)
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治療法の再整理とアップデートのために 専門家による私の治療 顎口虫症 Invited
丸山 治彦
日本医事新報 ( 5033 ) 40 - 41 2020.10
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：(株)日本医事新報社
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感染症の今日的問題点 寄生虫症からかいま見える現代日本社会 Invited
丸山 治彦
感染症学雑誌 94 ( 臨増 ) 106 - 106 2020.3
Authorship：Lead author Language：Japanese Publishing type：Rapid communication, short report, research note, etc. (scientific journal) Publisher：(一社)日本感染症学会
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病原診断法ー寄生虫感染症
丸山 治彦
小児科 57 ( 6 ) 591 - 598 2016.5
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：金原出版株式会社
Presentations 【 display / non-display 】
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わが国における食肉由来寄生虫症 Invited
丸山治彦
第160回日本獣医学会集会 食肉の安全性に関する市民公開シンポジウム 「食肉に由来する感染症とその予防」
Event date： 2017.9.13 - 2017.9.15
Language：Japanese Presentation type：Oral presentation (invited, special)
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わが国における食肉由来寄生虫症 International conference
丸山治彦
第160回日本獣医学会集会 食肉の安全性に関する市民公開シンポジウム 「食肉に由来する感染症とその予防」
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血清疫学的手法による鶏肉・内臓を介したヒトの動物由来回虫症への感染リスク評価
吉田彩子, Yen Thi HoangNguyen, 丸山治彦, 堀井洋一郎, 野中成晃
第159回日本獣医学会学術集会
Event date： 2016.9.6 - 2016.9.8
Language：Japanese Presentation type：Oral presentation (general)
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血清疫学的手法による鶏肉・内臓を介したヒトの動物由来回虫症への感染リスク評価 International conference
吉田彩子, Yen Thi HoangNguyen, 丸山治彦, 堀井洋一郎, 野中成晃
第159回日本獣医学会学術集会
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ウエステルマン肺吸虫症の原因食品としてのシカ肉の可能性
吉田彩子, 松尾加代子, 長安英治, 丸山治彦
第26回日本臨床寄生虫学会大会
Event date： 2016.6.20
Language：Japanese Presentation type：Oral presentation (general)
Grant-in-Aid for Scientific Research 【 display / non-display 】
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磁性ナノ粒子を用いた蠕虫のin vivoイメージングによる体内移行動態の解析
Grant number：21K06995 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(C)
吉田 彩子,
Authorship：Coinvestigator(s)
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寄生虫の分子同定と系統解析のための統一標準法の確立
Grant number：21H02725 2021.04 - 2024.03
独立行政法人日本学術振興会 科学研究費補助金 基盤研究(B)
Authorship：Principal investigator
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実臨床データを利用した寄生虫症最適検査診断システムの構築
2018 - 2021.03
科学研究費補助金 基盤研究(C)
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芽殖孤虫のゲノム解読による条虫類幼虫における無性増殖機構の解明
2014.04 - 2017.03
科学研究費補助金 基盤研究(C)
Authorship：Principal investigator
芽殖孤虫のゲノム解読による条虫類幼虫における無性増殖機構の解明
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出芽酵母を用いた寄生線虫由来の長寿遺伝子の単離
2012.04 - 2014.03
科学研究費補助金 挑戦的萌芽研究
Authorship：Principal investigator
出芽酵母を用いた寄生線虫由来の長寿遺伝子の単離
Other research activities 【 display / non-display 】
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ウガンダ
2013.05
国際協同研究打合せ（Gulu大学）
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寄生虫症薬物治療の手引き2010
2009.11 - 2010.04
熱帯病治療薬研究班の知見をまとめた、熱帯病および寄生虫疾患の治療ガイドラインの編集。わが国においては寄生虫病や熱帯病に対する薬剤は国内未承認あるいは保険適用外であることが多く、ガイドラインの編集作製が急務であったことに対応したもの。
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寄生虫症の血清疫学
2006.10
日本各地の医療機関からの寄生虫病血清診断依頼に対応しわが国の寄生虫病相を明らかにする