Papers - HIRAI Takuya
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Histopathological changes in the pancreas of cattle with abdominal fat necrosis
TANI Chikako, PRATAKPIRIYA Watanyoo, TANI Mineto, YAMAUCHI Takenori, HIRAI Takuya, YAMAGUCHI Ryoji, ANO Hitoshi, KATAMOTO Hiromu
Journal of Veterinary Medical Science 79 ( 1 ) 52 - 59 2017.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
DOI： 10.1292/jvms.16-0282
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Hidaka Yuichi, Tsuzuki Nao, Hagio Mitsuyoshi, Koike Kouji, Misumi Shun, Kikkawa Lisa, Konishi Yuko, Satoh Hiroyuki, Hirai Takuya, Mido Shogo, Horii Yoichiro
Journal of Japan Veterinary Cancer Society 6 ( 1 ) 5 - 12 2017
Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Veterinary Cancer Society
Non-selective intra-arterial (I-A) chemotherapy of paclitaxel plus platinum combination was performed in two dogs with cancers of the nasal cavity, including undifferentiated (Case 1) and transitional (Case 2) carcinomas. The drug dosages were reduced to 25% to 35.5% of the typical doses for systemic chemotherapy, and they were perfused via common carotid arteries. Case 1 had marked regression of the cancer but survived for only 53 days after the therapy. Case 2 had partial remission for approximately 3 months and survived for 126 days. Adverse events of the chemotherapies were not induced in Case 1, but mild adverse events were seen in Case 2. Non-selective I-A chemotherapy in this report required easy and simple procedures and could be safely performed via a reduction of the dosages of the drugs. Although future research will address issues concerning the intervals, frequencies, and drug dosages, the I-A chemotherapy was suggested as being able to contribute to palliative remission in canine intranasal cancers.
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Non-tuberculous Mycobacteriosis with T-cell Lymphoma in a Red Panda (Ailurus fulgens). Reviewed
Fuke N, Hirai T, Makimura N, Goto Y, Habibi WA, Ito S, Trang NT, Koshino K, Takeda M, Yamaguchi R
Journal of comparative pathology 155 ( 2-3 ) 263 - 266 2016.8
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Comparative Pathology
A 9-year-old male red panda (Ailurus fulgens) became emaciated and died. Necropsy examination revealed systemic lymphadenomegaly. The liver, lungs and left kidney contained multifocal yellow nodules. Microscopical examination revealed granulomatous inflammation in the liver, lungs, kidney, spleen and lymph nodes, with numerous acid-fast bacilli. Sequencing of genetic material isolated from the tissues classified the pathogen as Mycobacterium gastri. Lymphoma was found in the liver, lungs, kidney and lymph nodes. The neoplastic cells were strongly labelled for expression of CD3, Ki67 and proliferating cell nuclear antigen by immunohistochemistry. This is the first report of M. gastri infection with T-cell lymphoma in a red panda.
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Non-tuberculous Mycobacteriosis with T-cell Lymphoma in a Red Panda (Ailurus fulgens). Reviewed
Fuke N, Hirai T, Makimura N, Goto Y, Habibi WA, Ito S, Trang NT, Koshino K,
J Comp Pathol. 155 263 - 266 2016.7
Language：English Publishing type：Research paper (scientific journal)
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Pathological Characterization of an Outbreak of Porcine Reproductive and Respiratory Syndrome in Northern Vietnam. Reviewed
Huong Giang NT, Lan NT, Nam NH, Hirai T, Yamaguchi R.
J Comp Pathol. 154 135 - 149 2016.6
Language：English Publishing type：Research paper (scientific journal)
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Huong Giang N.T., Lan N.T., Nam N.H., Hirai T., Yamaguchi R.
Journal of Comparative Pathology 154 ( 2-3 ) 135 - 149 2016.2
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Comparative Pathology
In 2007, a highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) emerged in Vietnam and spread to nearly all regions of the country by 2010. Ten representative pigs of different age groups, infected naturally with HP-PRRSV in northern Vietnam in 2010, were used to characterize the pathological features of the infection. Infection was confirmed using reverse transcriptase polymerase chain reaction and viral isolation. The clinical signs and gross findings in these pigs included high fever (>40.2°C), red skin, blue ears, anorexia, respiratory distress, diarrhoea, haemorrhagic pleurisy and lymphadenopathy. Reproductive failure was the main clinical feature in sows. PRRSV infection-associated microscopical lung and lymph node lesions were observed frequently, regardless of age of the animals. Lung lesions were characterized by interstitial pneumonia and were occasionally associated with haemorrhage and fluid exudation following alveolar collapse. Lymph nodes exhibited characteristic haemorrhage and apoptosis, lymphocytic depletion and disorganization secondary to fibrosis and capillary formation. Haematoxylin and eosin staining or caspase-3 immunohistochemistry revealed apoptosis induction in various tissues and organs, particularly the lymph nodes and lungs. Primarily haemorrhagic microscopical lesions were observed commonly in other organs including the spleen, liver, heart and kidney. Immunohistochemical examination revealed HP-PRRS antigen in the lung, lymph node, liver and kidney macrophages, and lung and kidney epithelial cells. Pigs infected naturally with HP-PRRS in the field have multisystemic disease characterized by marked apoptotic cell death.
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US-like isolates of porcine epidemic diarrhea virus from Japanese outbreaks between 2013 and 2014.
Van Diep N, Norimine J, Sueyoshi M, Lan NT, Hirai T, Yamaguchi R
SpringerPlus 4 756 2015
Language：Japanese Publishing type：Research paper (scientific journal)
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Membranoproliferative glomerulonephritis in a calf with nephrotic syndrome. Reviewed
Trang NT, Hirai T, Nabeta R, Fuke N, Yamaguchi R.
J Comp Pathol. 151 162 - 165 2014.7
Language：English Publishing type：Research paper (scientific journal)
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A Canine Case of Ovarian Carcinoma with Pulmonary Metastasis Treated with Surgery and Paclitaxel / Platinum Combination Chemotherapy. Reviewed
Hidaka Y., Kodama A., Tamura R., Ito M., Koike K., Hirai T., Satoh H. and Hagio M.
J. Jpn. Vet. Cancer. Soc. 5 ( 1 ) 6 - 11 2014.2
Language：English Publishing type：Research paper (scientific journal)
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Trang N.T., Hirai T., Yamamoto T., Matsuda M., Okumura N., Giang N.T.H., Lan N.T., Yamaguchi R.
Journal of Veterinary Science 15 ( 3 ) 361 - 367 2014
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Veterinary Science
The objectives of the present study were to evaluate the anatomic localization of porcine reproductive and respiratory syndrome virus (PRRSV) in naturally infected pigs and to determine whether oral fluid could be used to detect the virus in infected animals. Two sows, seven 2-month-old grower pigs, and 70 6-month-old gilts were included in this study. PRRSV in sera and oral fluid were identified by nested reverse transcription PCR (nRT-PCR) while lung, tonsil, and tissue associated with oral cavity were subjected to nRT-PCR, immunohistochemistry, and in situ hybridization. In sows, PRRSV was identified in oral fluid and tonsils. PRRSV was also detected in oral fluid, tonsils, salivary glands, oral mucosa, and lungs of all seven grower pigs. However, viremia was observed in only two grower pigs. Double staining revealed that PRRSV was distributed in macrophages within and adjacent to the tonsillar crypt epithelium. In gilts, the North American type PRRSV field strain was detected 3 to 8 weeks after introducing these animals onto the farm. These results confirm previous findings that PRRSV primarily replicates in tonsils and is then shed into oral fluid. Therefore, oral fluid sampling may be effective for the surveillance of PRRSV in breeding herds.
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Hidaka Yuichi, Kodama Akihito, Tamura Ryota, Ito Masafumi, Koike Kouji, Hirai Takuya, Satoh Hiroyuki, Hagio Mitsuyoshi
Journal of Japan Veterinary Cancer Society 5 ( 1 ) 6 - 11 2014
Language：English Publishing type：Research paper (scientific journal) Publisher：Japan Veterinary Cancer Society
A five-year-old female Miniature Dachshund was presented with abdominal distension and dyspnea. Physical, radiographical, and ultrasound examinations detected an abdominal mass and pleural effusion. The cytology of the mass and effusion indicated a malignant tumor, possibly of ovarian origin, with intrathoracic metastasis. Laparotomy demonstrated that the mass originated from the left ovary, and a complete ovariohysterectomy was performed. The histopathological examination revealed papillary adenocarcinoma in both ovaries. Paclitaxel/platinum combination chemotherapy was administered nine times to control pleural effusion, and the dog survived 623 days after the surgery. The postmortem examination confirmed that the metastatic tumor had spread to the lungs. Paclitaxel/platinum combination chemotherapy may be an acceptable option for treatment in dogs with metastatic ovarian adenocarcinoma.
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Antimicrobial resistance in Salmonella serovars isolated from meat shops at the markets in North Vietnam.
Thai TH, Lan NT, Hirai T, Yamaguchi R
Foodborne pathogens and disease 9 ( 11 ) 986 - 91 2012.11
Language：Japanese Publishing type：Research paper (scientific journal)
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Pathological effects in lymphoid tissues of the spleen, lymph nodes, and Peyer's patches in cyclosporin-treated cynomolgus monkeys.
Moriyama A, Maeda H, Hirai T, Yamaguchi R
The Journal of veterinary medical science 74 ( 11 ) 1487 - 91 2012.11
Language：Japanese Publishing type：Research paper (scientific journal)
DOI： 10.1292/jvms.12-0155
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Nectin4 is an epithelial cell receptor for canine distemper virus and involved in neurovirulence.
Pratakpiriya W, Seki F, Otsuki N, Sakai K, Fukuhara H, Katamoto H, Hirai T, Maenaka K, Techangamsuwan S, Lan NT, Takeda M, Yamaguchi R
Journal of virology 86 ( 18 ) 10207 - 10 2012.9
Language：Japanese Publishing type：Research paper (scientific journal)
DOI： 10.1128/JVI.00824-12
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Antimicrobial resistance of Salmonella serovars isolated from beef at retail markets in the north Vietnam. Reviewed
Thai TH, Hirai T, Lan NT, Shimada A, Ngoc PT, Yamaguchi R
The Journal of veterinary medical science 74 ( 9 ) 1163 - 9 2012.9
Language：English Publishing type：Research paper (scientific journal)
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Thai T.H., Hirai T., Lan N.T., Shimada A., Ngoc P.T., Yamaguchi R.
Journal of Veterinary Medical Science 74 ( 9 ) 1163 - 1169 2012.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Veterinary Medical Science
Approximately 39.9% (63/158) of beef samples collected from retail markets in Hanoi from January to June 2009 were Salmonella- positive. Nine Salmonella serovars, Anatum (28.6%), Rissen (25.4%), Weltevreden (12.7%), Typhimurium (7.9%), Derby (7.9%), Lexington (7.9%), Dublin (4.6%), Newport (3.2%) and London (1.8%), were identified. Thirty-seven (58.7%) of the 63 Salmonella isolates were resistant to at least one antimicrobial tested, of which 29 (46%) isolates showed multidrug resistance (MDR). The isolates were commonly resistant to tetracycline (46.0%), sulphonamide (39.7%), ampicilline (31.7%), streptomycin (30.2%), trimethoprim (28.6%), kanamycin (28.6%) and chloramphenicol (22.2%). Fourteen (bla , bla , aadA1, aadA2, sul1, tetA, tetB, tetG, cmlA1, floR, dfrA1, dfrA12, aac (3)-IV and aphA1-1AB) out of 22 antimicrobial resistance genes were detected by PCR from the resistant isolates. The catA1, Kn, bla genes and plasmid-mediated quinolones resistance (PMQR) genes such as qnrA, qnrB, qnrS, qepA and acc (6')-ib-cr were not detected. Mutations in the gyrA gene leading to the amino acid changes Ser83Phe and/or Asp87Asn were found in 6 out of the 11 quinolone-resistant isolates. The data revealed that multidrug resistant Salmonella strains were widely distributed in north Vietnam via the food chain and might contain multiple genes specifying identical resistant phenotypes. Thus, continuous studies are necessary to clarify the mechanisms of MDR in Salmonella and its spread in the livestock market. ©2012 The Japanese Society of Veterinary Science. TEM OXA-1 PSE-1
DOI： 10.1292/jvms.12-0053
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Antibiotic resistance profiles of Salmonella serovars isolated from retail pork and chicken meat in North Vietnam.
Thai TH, Hirai T, Lan NT, Yamaguchi R
International journal of food microbiology 156 ( 2 ) 147 - 51 2012.5
Language：Japanese Publishing type：Research paper (scientific journal)
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Quantitative analyses of lymphoid tissue in the spleen, lymph nodes and Peyer's patches in cynomolgus monkeys.
Moriyama A, Fujishima J, Furukawa T, Yoshikawa T, Kodama R, Sasaki Y, Nagaoka T, Kamimura Y, Maeda H, Hirai T, Yamaguchi R
The Journal of veterinary medical science 73 ( 11 ) 1459 - 64 2011.11
Language：Japanese Publishing type：Research paper (scientific journal)
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Yamaguchi R., Moriyama A., Fujishima J., Furukawa T., Yoshikawa T., Kodama R., Sasaki Y., Nagaoka T., Kamimura Y., Maeda H., Hirai T.
Journal of Veterinary Medical Science 73 ( 11 ) 1459 - 1464 2011.11
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Veterinary Medical Science
To clarify the morphological characteristics of the cynomolgus monkey immune system, we analyzed quantitative data on their lymphoid organs. Spleens, major lymph nodes and Peyer's patches were sampled from cynomolgus monkeys, and the lymphoid follicle and germinal center areas and percentages of CD3- and CD20-positive areas were calculated. All the organs analyzed showed large inter-individual variations in the sizes of lymphoid follicles and germinal centers. Lymphoid follicle in the spleen, submandibular lymph nodes and Peyer's patches showed no marked difference in size. Germinal center size in the mesenteric lymph nodes and Peyer's patches were significantly smaller than those in the spleen. Areas containing T cells were largest in the lymph nodes, while those containing B cells were largest in the spleen and Peyer's patches. The mean size of the splenic lymphoid follicle in cynomolgus monkeys is larger than that in rats and similar to that in humans. Based on the large individual variation and the characteristics of lymphoid organs, it is important to use cynomolgus monkeys in standard toxicity studies. Taking advantage of the characteristics of each species enables reliable evaluation of the immunologic system in standard toxicity studies.
DOI： 10.1292/jvms.11-0207
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Hepatic myelolipoma with systemic amyloidosis in a goose (Anser cygnoides domesticus) Reviewed
Suzuki T., Uetsuka K., Kusanagi K., Hirai T., Nunoya T., Doi K.
Journal of Veterinary Medical Science 72 ( 5 ) 670 - 671 2010.6
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Veterinary Medical Science
We report here a case of hepatic myelolipoma with systemic amyloidosis in a goose (Anser cygnoides domesticus), which died suddenly following the short history of weakness and greenish diarrhea. At necropsy, multiple yellowish-white foci were observed on the surface of the prominently enlarged liver. Histologically, there were multiple foci of adipose tissue admixed with myeloid elements in various proportions in the liver as well as amyloid deposition in several organs including the liver, intestine, spleen, kidney, and ovary. Ultrastructurally, erythroblast-like cells and myelocytes, which showed various stages of differentiation, were observed in the foci of the liver. These findings shared characteristics of hepatic myelolipoma which is very rare in birds.