Papers - HIRAI Takuya
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Degeneration of the octavus nerve observed in a stranded Risso's dolphin (Grampus griseus), infested with Crassicauda grampicola Reviewed
T. Morimitsu, M. Koono, T. Hirai and T. Kurita
Japan Cetology 20 7 - 12 2010.6
Language：Japanese Publishing type：Research paper (scientific journal)
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Hepatic Myelolipoma with Systemic Amyloidosis in a Goose (Anser cygnoides Reviewed
Suzuki T, Uetsuka K, Kusanagi K, Hirai T, Nunoya T, Doi K.
J Vet Med Sci 72 ( 5 ) 669 - 671 2010.1
Language：English Publishing type：Research paper (scientific journal)
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Hepatic Myelolipoma with Systemic Amyloidosis in a Goose (<i>Anser cygnoides domesticus</i>) Reviewed
SUZUKI Takayuki, UETSUKA Kouji, KUSANAGI Kouichi, HIRAI Takuya, NUNOYA Tetsuo, DOI Kunio
Journal of Veterinary Medical Science 72 ( 5 ) 669 - 671 2010
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
DOI： 10.1292/jvms.09-0457
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Multiplex PCR and multiplex RT-PCR for inclusive detection of major swine DNA and RNA viruses in pigs with multiple infections. Reviewed
Ogawa H, Taira O, Hirai T, Takeuchi H, Nagao A, Ishikawa Y, Tuchiya K, Nunoya T, Ueda S.
J Virol Methods 160 210 - 214 2009.9
Language：English Publishing type：Research paper (scientific journal)
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Ogawa H., Taira O., Hirai T., Takeuchi H., Nagao A., Ishikawa Y., Tuchiya K., Nunoya T., Ueda S.
Journal of Virological Methods 160 ( 1-2 ) 210 - 214 2009.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Virological Methods
Multiplex PCR and multiplex RT-PCR were developed to identify nine viruses in pigs with multiple infections. These viruses are: porcine circovirus type 2 (PCV2), suid herpesvirus 1, porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), Japanese encephalitis virus, porcine rotavirus A (PoRV-A), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and Getah virus. These methods were shown to be high specificity and sensitivity. In the clinical application, a total of 75 field samples were examined by our methods and previously reported methods for PCV2, PRRSV, TGEV, and PEDV. As a result, the detection rates of our multiplex PCR and multiplex RT-PCR were higher than those of the previously reported methods. Furthermore, it was confirmed that 24 PCV2 positive samples were co-infected with other viruses, 11 with PRRSV, 10 with PPV, 2 with PoRV-A, and 1 with TGEV by a combination of multiplex PCR and multiplex RT-PCR. PPV and PoRV-A were newly detected by multiplex PCR and multiplex RT-PCR. These results suggest that the combination of our multiplex PCR and multiplex RT-PCR is useful for rapid and accurate identification of nine major pathogenic viruses in pigs with multiple infections. © 2009 Elsevier B.V. All rights reserved.
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LAN Nguyen Thi, YAMAGUCHI Ryoji, HIRAI Takuya, KAI Kazushige, MORISHITA Kazuhiro
Journal of Veterinary Medical Science 71 ( 4 ) 457 - 461 2009.5
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
DOI： 10.1292/jvms.71.457
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Relationship between growth behavior in vero cells and the molecular characteristics of recent isolated classified in the Asia 1 and 2 groups of canine distemper virus. Reviewed
Lan NT, Yamaguchi R, Hirai T, Kai K, Morishita K.
J Vet Med Sci 71 ( 4 ) 457 - 461 2009.4
Language：English Publishing type：Research paper (scientific journal)
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Lan N.T., Yamaguchi R., Kien T.T., Hirai T., Hidaka Y., Nam N.H.
Journal of Veterinary Medical Science 71 ( 2 ) 155 - 162 2009.3
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Veterinary Medical Science
Canine distemper caused by canine distemper virus (CDV) is a contagious, incurable, often fatal, multisystemic viral disease that affects the respiratory gastrointestinal and central nervous system. Strains Vn86 and Vn99 of CDV were isolated, we believe for the first time, in Vietnam from two 4-month-old autopsied dogs pathologically showing non-suppurative encephalitis with pneumonia, lymphoid depletion and severe gastroenteritis. These strains caused syncytium cytopathic effect in Vero cells and Vero cells expressing canine signaling lymphocyte activation molecules. The titers of cell-associated viruses of both strains were higher than for released viruses. Molecular analysis showed that both new isolates of CDV joined to the group of classic type that is far from the Asia 1 and Asia 2 groups. These results indicated that first isolation and characterization of canine distemper virus in Vietnam with the immunohistochemical examination of the dog.
DOI： 10.1292/jvms.71.155
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First isolation and characterization of canine distemper virus in Vietnam with the immunohistochemical examination of the dog. Reviewed
Lan NT, Yamaguchi R, Kien TT, Hirai T, Hidaka Y, Nam NH.
J Vet Med Sci 71 ( 2 ) 155 - 162 2009.2
Language：English Publishing type：Research paper (scientific journal)
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Nakamura K., Shibuya K., Saito N., Shimada K., Ohshima A., Hirai T., Nunoya T.
Journal of Poultry Science 45 ( 4 ) 298 - 302 2008.11
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Poultry Science
Japanese quail (Cotumix coturnix japonica) is widely used in avian reproduction tests and avian sex reversal screening tests for evaluating adverse effects of endocrine disrupting chemicals (EDCs). However, there are few reports on the molecular biological profiles during the development and sexual differentiation of the gonads in Japanese quail. In the present study, we investigated mRNA expression of cytochrome P450 17αhydroxylase (P450c17), cytochrome P450 aromatase (P450 arom), anti-Mttllerian hormone (AMH), estrogen receptor α (ERα) and androgen receptor (AR) in the embryonic and post-hatching gonads of Japanese quail by using real-time RT-FCR. The left gonads of male and female Japanese quail at 16 days of incubation and 3, 7 and 14 days after hatching were used, in the study. P450c17 mRNA expression in females was significantly higher than that in males at 16 days of incubation, 3 and 7 days after hatching. P450arom mRNA expression in females was markedly higher than that in males and there were significant differences at 3, 7 and 14 days after hatching. AMH mRNA expression in males was apparently higher than that in females and there were significant differences at 16 days of incubation and 3 days after hatching. ERα mRNA was highly expressed not only in females but also in males at 16 days of incubation. There were no meaningful trends of AR mRNA expressions in male and female gonads during the observation periods. It is likely that the profiles of mRNA expression of P450c17, P450arom, AMH, ERα and AR in the embryonic and post-hatching gonads of normal Japanese quail in the present study provide basic and useful information. Copyright © 2008, Japan Poultry Science Association.
DOI： 10.2141/jpsa.45.298
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An undifferentiated sarcoma in a rat resembling extraskeletal myxoid chondrosarcoma in man.
Nakamura K, Shibuya K, Hirai T, Nunoya T.
Toxocol Pathol 35 ( 5 ) 672 - 675 2007.10
Language：English Publishing type：Research paper (scientific journal)
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An undifferentiated sarcoma in a rat resembling extraskeletal myxoid chondrosarcoma in man Reviewed
Nakamura K., Shibuya K., Hirai T., Nunoya T.
Toxicologic Pathology 35 ( 5 ) 672 - 675 2007.8
Language：English Publishing type：Research paper (scientific journal) Publisher：Toxicologic Pathology
A soft tissue tumor occurring in the inguinal subcutaneous tissue was detected in a 109-week-old male F344 rat. Macroscopically, the tumor mass showed no skeletal relationship and a gelatinous multinodular appearance. Histologically, the tumor consisted of irregular lobules separated by scant fibrous septa. In each lobule, tumor cells were arranged in cords and strands in the plentiful myxoid stroma. The tumor cells showed marked pleomorphism, and had large, round to ovoid nuclei and abundant eosinophilic cytoplasm. Mitotic figures were often seen. Myxoid stroma of the tumor contained a large amount of acid mucopolysaccharides and collagen type II, which were demonstrated by histochemistry and immunohistochemistry respectively. The tumor cells showed positive immunoreactivities for vimentin and S100 protein. Ultrastructural examination revealed that the tumor cells had a large amount of mitochondria, Golgi complex and dilated rough endoplasmic reticulum containing amorphous material, and the myxoid stroma contained collagenous fibrils and proteoglycan particles. Based on these results, the present tumor in a rat resembled extraskeletal myxoid chondrosarcoma in man. Copyright © by the Society of Toxicologic Pathology.
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Actinomycosis of the brain and temporal bone in a goat.
Hirai T, Nunoya T, Azuma R.
J Vet Med Sci 69 ( 6 ) 641 - 643 2007.6
Language：English Publishing type：Research paper (scientific journal)
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Actinomycosis of the brain and temporal bone in a goat Reviewed
Hirai T., Nunoya T., Azuma R.
Journal of Veterinary Medical Science 69 ( 6 ) 641 - 643 2007.6
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Veterinary Medical Science
A goat with neurologic signs had multifocal abscesses containing sulfur granules in the right brain and temporal bone. Histologically, the lesions consisted of pyogranulomas with several radiating bacterial colonies of various sizes. A tangled mass of filamentous and gram-positive bacteria was recognized in the central part of the colony. Actinomyces naeslundii antigen was detected in the colonies of bacteria in the brain and neighboring bone tissue by immunohistochemistry. Actinomycosis involving the central nervous system (CNS) and temporal bone is rare in animals. Cerebral infection with A. naeslundii may have resulted from direct extension from cervicofacial regions because the CNS lesions were distributed asymmetrically and were continuous with the right temporal bone.
DOI： 10.1292/jvms.69.641
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Use of miniature pig for biomedical research, with reference to toxicologic studies Reviewed
Tetsuo Nunoya, Kazumoto Shibuya, Toshiki Saitoh, Hajime Yazawa, Keigo Nakamura, Yasuko Baba, Takuya Hirai
J Toxicol Pathol 20 125 - 132 2007.5
Language：English Publishing type：Research paper (scientific journal)
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Koyama T., Hirai T., Nagai S.
Journal of Veterinary Medical Science 68 ( 6 ) 609 - 613 2006.7
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Veterinary Medical Science
Lawsonia intracellularis isolated from a Japanese field case of porcine proliferative enteropathy (PPE) was cultivated and partially characterized. The bacterial cells isolated from the intestinal mucosa of a pig suffering from the acute form of PPE were used to inoculate rat small intestine cells (IEC-18) and human epithelial cells (HEp-2). Infected foci, which were stained with L. intracellularis-specific rabbit antiserum, were observed in the cell culture at 5 days post inoculation. The DNA sequence of several genes in the Japanese isolate had high similarity with those of the L. intracellularis type strain, suggesting the genetically close relationship of the two strains. This is the first report describing the cultivation and partial characterization of L. intracellularis originated in Japan.
DOI： 10.1292/jvms.68.609
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Infectivity of porcine circovirus 1 and circovirus 2 in primary porcine hepatocyte and kidney cell cultures.
Hirai T, Nunoya T, Ihara T, Saitoh T, Shibuya K, Nakamura K.
J Vet Med Sci 68 ( 2 ) 179 - 182 2006.2
Language：English Publishing type：Research paper (scientific journal)
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Hirai T., Nunoya T., Ihara T., Saitoh T., Shibuya K., Nakamura K.
Journal of Veterinary Medical Science 68 ( 2 ) 179 - 182 2006.2
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Veterinary Medical Science
Infectivity of porcine circovirus (PCV) 1 and PCV2 was examined in primary porcine hepatocyte culture by comparing that of PCV in primary kidney cell culture. The virus titer of PCV2-infected hepatocyte cultures was higher than that of the PCV1-infected hepatocyte cultures and the PCV-infected kidney cell cultures. The number of virus-positive cells was most abundant in PCV2-infected hepatocyte cultures as determined by immunohistochemistry and/or in situ hybridization. The results of our data suggest that PCV2 preferably infects cultured hepatocytes as observed in the liver of pigs with postweaning multisystemic wasting syndrome.
DOI： 10.1292/jvms.68.179
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In vitro cultivation and partial characterization of Lawsonia intracellularis from a Japanese field case of porcine proliferative enteropathy.
Koyama T, Hirai T, Nagai S.
J Vet Med Sci 68 ( 6 ) 609 - 613 2006.1
Language：English Publishing type：Research paper (scientific journal)
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Acute hepatitis in a piglet experimentally inoculated with tissue homogenates from pigs with postweaning multisystemic wasting syndrome.
Hirai T, Nunoya T, Ihara T, Kusanagi K, Kato T, Shibuya K.
J Vet Med Sci 65 ( 9 ) 1041 - 1045 2003.9
Language：English Publishing type：Research paper (scientific journal)