Papers - UEMURA Ryoko
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A highly sensitive method for detecting African swine fever virus in oral fluids from naturally infected pigs in Northern Vietnam Reviewed International coauthorship
Thi Ngan Mai, Thi Huong Giang Tran, Van Hieu Dong, Van Phan Le, Thi My Le Huynh, Tran Anh Dao Bui, Ryoko Uemura, Yasuko Yamazaki, Thi Lan Nguyen & Wataru Yamazaki
Scientific Reports 15 27855 2025.7
Language：English Publishing type：Research paper (scientific journal)
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Comprehensive serotyping of Mannheimia haemolytica by a PCR system using the diversity of capsule biosynthesis genes. Reviewed
Iguchi A, Ueno Y, Hoshinoo K, Okuno M, Uemura R, You G, Ogura Y, Takamatsu D
Scientific reports 15 ( 1 ) 11970 2025.4
Language：English Publishing type：Research paper (scientific journal)
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Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes Reviewed
Nishi K., Gondaira S., Hirano Y., Ohashi M., Sato A., Matsuda K., Iwasaki T., Kanda T., Uemura R., Higuchi H.
Veterinary research 56 ( 1 ) 22 2025.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Veterinary research
Mycoplasma pneumonia, caused by Mycoplasma bovis (Mycoplasmopsis bovis; M. bovis), is linked with severe inflammatory reactions in the lungs and can be challenging to treat with antibiotics. Biofilms play a significant role in bacterial persistence and contribute to the development of chronic lesions. A recent study has shown that polymicrobial interactions between species are an important factor in biofilm formation, yet the precise mechanism of biofilm formation in M. bovis remains unknown. By assuming multiple pathogen infections in the bovine respiratory disease complex (BRDC), this study examined the characterisation of the polymicrobial relationship between M. bovis and Trueperella pyogenes (T. pyogenes) during biofilm formation. Autopsies were performed on four Holstein calves (two chronic Mycoplasma pneumonia calves and two control calves). Bacterium-like aggregation structures (> 10 μm), which were assumed to be biofilms of M. bovis in vivo, were observed adhering to the cilia in calves with Mycoplasma pneumonia. M. bovis released an extracellular matrix to connect with neighbouring bacteria and form a mature biofilm on the plate. Biofilm formation in the co-culture of M. bovis and T. pyogenes (strain T1: 1 × 105 and 1 × 106 CFU/well) significantly increased (p < 0.05 and p < 0.01; 64.1% and 64.8% increase) compared to that in a single culture of these bacteria. Furthermore, some large aggregates (> 40 μm), composed of M. bovis and T. pyogenes, were observed. The morphological characteristics of this biofilm were similar to those observed in vivo compared to a single culture. In conclusion, the polymicrobial interaction between M. bovis and T. pyogenes induces biofilm formation, which is associated with increased resistance to antimicrobial agents, and this exacerbates the progression of chronic Mycoplasma pneumonia.
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Efficacy of Treating Bacterial Bioaerosols with Weakly Acidic Hypochlorous Water: A Simulation Chamber Study Reviewed
Norkaew S, Narikawa S, Nagashima U, Uemura R, Noda J
Heliyon 2024.1
Language：English Publishing type：Research paper (scientific journal)
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A case of urachal abscess resection via colpotomy in a cow Reviewed
SATO Reiichiro, SATO Yukiko, FUKE Naoyuki, UEMURA Ryoko, STEINER Adrian
Journal of Veterinary Medical Science 86 ( 11 ) 1156 - 1161 2024
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
This study describes a novel surgical method to remove urinary bladder-related urachal abscesses in a cow. Traditional methods, such as the midline or paramedian approach in calves and the preinguinal approach, can be challenging in adult cattle, especially for lesions situated near the pelvic cavity. In this case, a 4-year-old cow had a urachal abscess attached to the bladder apex, making it difficult to approach via abdominal wall incision. Colpotomy and pulling the abscess through a vaginal wall incision allowed for complete exteriorization of the lesion and comfortable accessibility of the abscess and the resection site. This report suggests that colpotomy is an effective new route in adult cattle for accessing urachal abscesses not accessible via traditional surgical approaches.
DOI： 10.1292/jvms.24-0256
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Sukmawinata E., Uemura R., Nishiki I., Yoshida T., Sueyoshi M., Kikuchi T.
Veterinarski Glasnik 78 ( 1 ) 81 - 87 2024
Publishing type：Research paper (scientific journal) Publisher：Veterinarski Glasnik
Plasmids play an important role in the spread of antimicrobial resistance genes (ARGs) in bacteria. This study reports the complete sequence of the IncX3 plasmid identified in Escherichia coli isolated from faeces of a Japanese racehorse. Sequence analysis revealed that this plasmid harbours the blaSHV-12 gene, which encodes an extended spectrum β-lactamase, and the quinolone resistance gene qnrS1. The IncX3 plasmids carrying ARGs have been previously identified in bacteria isolated from humans, animals and a variety of environments. This is the first report of the complete sequence of the IncX3 plasmid carrying blaSHV-12 and qnrS1 genes from a Japanese racehorse, which provides insights into understanding the spread and mechanism of antimicrobial resistance particularly in Japanese racehorses.
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Shitamori F., Uemura R., Kanda T., Sueyoshi M.
Open Veterinary Journal 12 ( 6 ) 782 - 786 2022.11
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Open Veterinary Journal
Background: Mycoplasma bovis causes various diseases such as bronchopneumonia, otitis media, arthritis, and mastitis in cattle. Mycoplasma bovis is often isolated from the deep pharynges of healthy cattle and is generally considered not to cause clinical symptoms while in the upper respiratory tract. In mycoplasma infections, adhesion to the host cells is a crucial step. In recent years, five new adhesins, NOX, α-enolase, TrmFO, P27, and VpmaX, have been reported in M. bovis strains from pneumonia cases. However, the presence of these adhesins in wild isolates has not been established. Aim: This study aimed to investigate the presence of these adhesin genes in wild isolates isolated from cattle nasal cavities and lesion sites (pneumonia, otitis media, arthritis, and mastitis) in various regions in Japan and clarify the relationship between adhesion and the symptoms caused by M. bovis infection. Methods: A total of 141 M. bovis wild isolates isolated from nasal cavities (healthy or sick cattle), lungs with pneumonia, ears with otitis media, joint fluids of arthritic animals, and milk of mastitic animals. Mycoplasma bovis type strain PG45 was also used. Specific polymerase chain reaction reactions were performed to detect nox, α-enolase, trmFO, P27, and vpmaX, which are adhesins of M. bovis. Results: This study reports 139 M. bovis wild isolates were positive for nox, α-enolase, trmFO, P27, and vpmaX, while two isolates each lacked α-enolase or P27 genes. Mycoplasma bovis PG45 also had all five adherens genes. Conclusion: Almost all M. bovis wild isolates possessed all nox, α-enolase, trmFO, P27, and vpmaX genes regardless of the lesion site or region of origin. This means no relationship was found between the presence of the five adhesins and lesion sites in M. bovis and M. bovis isolated from the nasal cavities of asymptomatic cattle have the same numbers and types of adhesins as isolates from symptomatic lesion sites (pneumonia, otitis media, arthritis, and mastitis). This suggests that not only M. bovis isolates from pulmonary lesions, but also M. bovis existing in the nasal cavity has the potential to causes symptoms in the host.
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Effects of Probiotics on Growth and Immunity of Piglets. Reviewed
Azizi AFN, Uemura R, Omori M, Sueyoshi M, Yasuda M
Animals : an open access journal from MDPI 12 ( 14 ) 2022.7
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.3390/ani12141786
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Mathurot Suwanruengsri Ryoko Uemura Uda Zahli Izzati Takuya Kanda Naoyuki Fuke Masahiro Yasuda Takuya Hirai Ryoji Yamaguchi
Thai J Vet Med. 52 ( 1 ) 33 - 44 2022.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Comparative Pathology
Mycoplasma hyopneumoniae (Mhp) is the primary pathogen of porcine enzootic pneumonia (PEP). Consolidated lung tissue from the cranioventral lung lobes of 15 pigs with PEP was collected for quantitative polymerase chain reaction, histopathology and immunohistochemistry. Histopathology revealed the co-existence of bronchial-associated lymphoid tissue hyperplasia with intra-alveolar neutrophils and macrophage infiltration in lesions of suppurative bronchopneumonia. Immunolabelling of infiltrated macrophages with CD163/CD204 indicated the presence of M2-polarized macrophages. Mhp antigen was detected on respiratory epithelial cells and in phagocytosed neutrophils. The intensity of Mhp immunolabelling and number of CD163/CD204-positive macrophages were correlated with the Mhp load in lung tissue (r = 0.87, 0.56, P <0.05). IL-8 immunolabelling was mainly found in neutrophils and correlated with Mhp load, Mhp immunolabelling and histological lesion score (r = 0.70, 0.66, 0.64, P <0.05), respectively. Apoptosis was seen in intra-alveolar cells and was correlated with Mhp load (r = 0.62, P <0.05). It is postulated that IL-8 attracts neutrophils to the lesions, while M2-polarized macrophages are a major source of IL-10 and promote a Th2-type immune response.
DOI： 10.14456/tjvm.2022.4
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Optimizing antimicrobial use by improving medication adherence among pig producers Reviewed
Toya, Ryohei; Sasaki, Yosuke; Uemura, Ryoko; Sueyoshi, Masuo
Animal Science Journal 2022
Language：English Publishing type：Research paper (scientific journal)
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Antibiotic-resistant Escherichia coli isolated from dairy cows and their surrounding environment on a livestock farm practicing prudent antimicrobial use Reviewed
Yoshihiro Suzuki*, Hayate Hiroki, Hui Xie, Masateru Nishiyama, Shinsuke H. Sakamoto, Ryoko Uemura, Kei Nukazawa, Yoshitoshi Ogura, Toru Watanabe, and Ikuo Kobayashi
International Journal of Hygiene and Environmental Health 2022
Language：English Publishing type：Research paper (scientific journal)
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Eimeria pragensis infection alters the gut microenvironment to favor extrinsic shiga toxin-producing Escherichia coli O157:H7 colonization in mice Reviewed
Ryoko Uemura, Miki Kawakado, Masuo Sueyoshi, Nariaki Nonaka and Yoichiro Horii
Parasitology International 2022
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Interferon gamma, lipopolysaccharide, and modified-live viral vaccines stimulation alter the mRNA expression of tumor necrosis factor α, inducible nitric oxide synthase, and interferon β in bovine alveolar macrophages Reviewed
Shingo Ishikawa, Masataka Miyazawa, Chiho Tanaka, Ryoma Uesawa, Juri Nishizawa, Ryoko Uemura, Ikuo Kobayashi, Seiji Hobo
Veterinary Immunology and Immunopathology 2022
Language：English Publishing type：Research paper (scientific journal)
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Protective effects of probiotics against tannin-induced immunosuppression in broiler chickens Reviewed
RAMAH Amany, YASUDA Masahiro, OHASHI Yuki, IMATAKE Shoichiro, IMAIZUMI Noriko, KIDA Tetsuo, YANAGITA Tenya, UEMURA Ryoko, BAAKHTARI Mahmoud, BAKRY Hatem H., ABDELALEEM Nabila M., EL-SHEWY Elham A.
Bifidobacteria and Microflora advpub ( 0 ) 2022
Language：English Publishing type：Research paper (scientific journal) Publisher：BMFH出版会
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Production of granulomas in <i>Mycoplasma bovis</i> infection associated with meningitis-meningoencephalitis, endocarditis, and pneumonia in cattle. Reviewed
Suwanruengsri M, Uemura R, Kanda T, Fuke N, Nueangphuet P, Pornthummawat A, Yasuda M, Hirai T, Yamaguchi R
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 10406387211053254 2021.11
Language：English Publishing type：Research paper (scientific journal)
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Nueangphuet P., Suwanruengsri M., Fuke N., Uemura R., Hirai T., Yamaguchi R.
Journal of Comparative Pathology 189 31 - 44 2021.11
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Comparative Pathology
Mycoplasma hyopneumoniae (Mhp) is the primary pathogen of porcine enzootic pneumonia (PEP). Consolidated lung tissue from the cranioventral lung lobes of 15 pigs with PEP was collected for quantitative polymerase chain reaction, histopathology and immunohistochemistry. Histopathology revealed the co-existence of bronchial-associated lymphoid tissue hyperplasia with intra-alveolar neutrophils and macrophage infiltration in lesions of suppurative bronchopneumonia. Immunolabelling of infiltrated macrophages with CD163/CD204 indicated the presence of M2-polarized macrophages. Mhp antigen was detected on respiratory epithelial cells and in phagocytosed neutrophils. The intensity of Mhp immunolabelling and number of CD163/CD204-positive macrophages were correlated with the Mhp load in lung tissue (r = 0.87, 0.56, P <0.05). IL-8 immunolabelling was mainly found in neutrophils and correlated with Mhp load, Mhp immunolabelling and histological lesion score (r = 0.70, 0.66, 0.64, P <0.05), respectively. Apoptosis was seen in intra-alveolar cells and was correlated with Mhp load (r = 0.62, P <0.05). It is postulated that IL-8 attracts neutrophils to the lesions, while M2-polarized macrophages are a major source of IL-10 and promote a Th2-type immune response.
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Mycoplasma bovis may travel along the eustachian tube to cause meningitis in Japanese black cattle Reviewed
Suwanruengsri M., Uemura R., Izzati U.Z., Kanda T., Fuke N., Yasuda M., Hirai T., Yamaguchi R.
Journal of Comparative Pathology 188 13 - 20 2021.10
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of Comparative Pathology
Mycoplasma bovis (M. bovis) is a common inhabitant of the upper and lower respiratory tracts of cattle and is considered to be the main aetiological agent of otitis media in calves. The eustachian tube appears to be the most common portal for pathogens to enter the middle ear. We investigated the transmission route of M. bovis causing otitis media that progressed to meningitis or meningoencephalitis in Japanese Black cattle. M. bovis was detected in 10 cases by a loop-mediated isothermal amplification method or by immunohistochemistry. One case of caseonecrotic granulomatous meningoencephalitis, one case of caseonecrotic granulomatous meningitis, one case of suppurative meningoencephalitis, eight cases of eustachitis, nine cases of tonsillitis and six cases of suppurative bronchopneumonia were identified by histopathological examination. M. bovis antigen was detected in the eustachian tubes of eight cases. In nine cases, M. bovis was also detected in tonsillar epithelial crypts and lumina, in intraluminal inflammatory cells and in the epithelial cells of minor salivary glands located around the eustachian tubes and tonsils. The results suggest that M. bovis can infect and colonize the tonsils and enter the eustachian tubes, causing otitis media, which, in cases of chronic infection, can progress to meningitis.
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Fujimoto Y., Inoue H., Kanda T., Ijiri M., Uemura R.
Avian diseases 65 ( 3 ) 401 - 405 2021.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Avian diseases
Colibacillosis, an infectious disease of chickens, is caused by avian pathogenic Escherichia coli (APEC); however, in addition to APEC, other pathogens are also frequently isolated from chickens affected with colibacillosis. Therefore, experimental infections in chickens are necessary to evaluate the pathogenicity of APEC isolates. Recent studies have shown that embryo lethality assays can be used as an alternative method to evaluate the pathogenicity of E. coli. In this study, to determine the important virulence genes associated with the pathogenicity of E. coli, 67 strains of E. coli that possessed different combinations of eight representative virulence genes (cva/cvi, vat, tsh, iucD, papC, irp2, iss, and astA) were isolated from broilers with colibacillosis in Japan, and the chicken embryo lethal assay was conducted. The genes vat, papC, and irp2 showed strong correlation with the level of virulence in E. coli. Our study provides useful information about the important virulence-associated genes in relation to the pathogenicity of E. coli in Japanese chickens.
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Toya R, Sasaki Y, Uemura R, Sueyoshi M
The Journal of veterinary medical science 83 ( 2 ) 322 - 328 2021.2
Language：English Publishing type：Research paper (scientific journal) Publisher：公益社団法人 日本獣医学会
In Japan, reducing the use of antimicrobials in pig production is a significant problem. However, there are no published reports concerning porcine disease treatment, as related to the age of the pigs and the indications (e.g., organ system) in Japan. In this study, we analyzed the prescription records of 17 farrow-to-finish farms from 2014 to 2018 in southern Kyushu, Japan. The farms' antimicrobial usage was calculated as the active ingredient per population correction unit (PCU) or the number of treated pigs per PCU using the defined daily dose. All data were analyzed according to the indications and production stages (sows, suckling pigs, post-weaning pigs, and fattening pigs). In terms of active ingredients/PCU, tetracyclines were the most commonly used (43.2–59.3%), and the largest amounts of antimicrobials administered through feeds were for treating the respiratory organs of fattening pigs. In terms of the number of treated pigs/PCU, tetracyclines were most frequently used (16.3–31.1%), and a high frequency of antimicrobials administered through feeds was used for the treatment of respiratory organs in post-weaning pigs. In this study, it was confirmed that tetracyclines were used frequently as a herd treatment for respiratory diseases in post-weaning and fattening pigs in southern Kyushu, Japan. The findings suggest that it is necessary to improve the treatment and prevention of respiratory diseases in post-weaning and fattening pigs in order to reduce the frequencies of antimicrobial treatments.
DOI： 10.1292/jvms.20-0436
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Radiographic diagnosis and surgical management for successful outcomes for osteochondrosis in Japanese Black calves. Reviewed
Vishwanath, NM., Kirino, Y., Uemura, R., Kim, S., Inoue, I., Hidaka, Y.
The Journal of Veterinary Medical Science. 83 ( 1 ) 151 - 157 2021.1
Language：English Publishing type：Research paper (scientific journal)
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細菌性皮膚感染症の犬から分離したStaphylococcus pseudintermediusの2008～2018年におけるセフォベシンナトリウム（コンベニア®注）に対する薬剤感受性調査成績 Reviewed
松本哲、裵東焄、上村涼子、三澤尚明、橋口順子、岩花倫生、藤原すばる、島田尚樹、西藤公司.
獣医臨床皮膚科 2021
Language：Japanese Publishing type：Research paper (scientific journal)
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Swine consolidated pneumonia in natural infection associated with Mycoplasma hyopneumoniae demonstrating neutrophil and M2-polarized macrophage infiltration and expression of IL-8 and apoptosis. Reviewed
Nueangphuet, P., Suwanruengsri, M., Fuke, N., Uemura, R., Hirai, T., Yamaguchi R.
Journal of Comparative Pathology 2021
Language：English Publishing type：Research paper (scientific journal)
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Antimicrobial Resistance Profiles and Phylogenetic Groups of Escherichia coli Isolated from Healthy Thoroughbred Racehorses in Japan Reviewed
Wataru Sato, Eddy Sukmawinata, Ryoko Uemura, Takuya Kanda, Kanichi Kusano, Yoshinori Kambayashi, Takashi Sato, Yuhiro Ishikawa, Ryohei Toya, Masuo Sueyoshi
Journal of Equine Science 2020.12
Language：English Publishing type：Research paper (scientific journal)
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Sukmawinata E., Sato W., Uemura R., Kanda T., Kusano K., Kambayashi Y., Sato T., Ishikawa Y., Toya R., Sueyoshi M.
Journal of Equine Veterinary Science 94 2020.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of Equine Veterinary Science
© 2020 Elsevier Inc. In this study, the occurrence of antimicrobial-resistant (AMR) enterococci was evaluated in Thoroughbred (TB) racehorses in Japan. Fecal samples were collected from 212 healthy TB racehorses at the Miho and Ritto Training Centers of the Japan Racing Association from March 2017 to August 2018. Isolation and identification were performed by enterococcus selective medium and confirmed to the species using MALDI-TOF MS. Enterococcus faecium and E. faecalis isolates were subjected to antimicrobial susceptibility test against 11 antimicrobials by minimum inhibitory concentration based on recommendation from Clinical and Laboratory Standards Institute guidelines. Among 583 enterococcus isolates, E. faecium and E. faecalis were identified for 48.2% (281/583) and 7.4% (43/583), respectively. One isolate that was representing E. faecium (153 isolates) and E. faecalis (31 isolates) from each sample was selected for antimicrobial susceptibility test. The highest rate of resistance for E. faecium isolates was observed against enrofloxacin (57.5%; 88/153), followed by streptomycin (32.0%; 49/153), kanamycin (18.3%; 28/153), gentamycin (5.9%; 9/153), erythromycin (5.9%; 9/153), and oxytetracycline (4.6%; 7/153). For E. faecium isolates, the highest resistance was observed against streptomycin (90.3%; 28/31), followed by kanamycin (41.9%; 13/31), gentamycin (29.0%; 9/31), lincomycin (9.7%; 3/31), oxytetracycline (6.5%; 2/31), erythromycin (6.5%; 2/31), tylosin (6.5%; 2/31), enrofloxacin (6.5%; 2/31), and chloramphenicol (3.2%; 1/31). The results indicated that enrofloxacin and aminoglycosides were highly resistant among tested antimicrobials. Continuous monitoring studies are useful to increase the awareness of the potential for AMR bacteria to arise from imprudent use of antimicrobials in TB racehorses in Japan.
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IncI1 Plasmid Associated with blaCTX-M-2 Transmission in ESBL producing E. coli Isolated from Healthy Thoroughbred Racehorse, Japan Reviewed
Eddy Sukmawinata, Ryoko Uemura, Wataru Sato, Shuya Mitoma, Takuya Kanda and Masuo Sueyoshi
Antibiotics 2020.4
Language：English Publishing type：Research paper (scientific journal)
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Sukmawinata E., Uemura R., Sato W., Htun M.T., Sueyoshi M.
Animals 10 ( 3 ) 2020.3
Language：English Publishing type：Research paper (scientific journal) Publisher：Animals
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Extended-spectrum β-lactamase (ESBL)- and AmpC β-lactamase (AmpC)-producing Klebsiella spp. have become a major health problem, leading to treatment failure in humans and animals. This study aimed to evaluate the presence of ESBL/AmpC-producing Klebsiella spp. isolated from racehorses in Japan. Feces samples from 212 healthy Thoroughbred racehorses were collected from the Japan Racing Association Training Centers between March 2017 and August 2018. ESBL/AmpC-producing Klebsiella spp. were isolated using selective medium containing 1 μg/mL cefotaxime. All isolates were subjected to bacterial species identification (MALDI-TOF MS), antimicrobial susceptibility test (disk diffusion test), characterization of resistance genes (PCR), conjugation assay, and genetic relatedness (multilocus sequence typing/MLST). Twelve ESBL/AmpC-producing Klebsiella pneumoniae (ESBL/AmpC-KP) were isolated from 3.3% of horse samples. Antimicrobial resistance profiling for 17 antimicrobials showed all ESBL/AmpC-KP were multidrug-resistant (MDR). Only 1 isolate was confirmed as an ESBL producer (blaCTX-M-2-positive), whereas the other 11 isolates were plasmid-mediated AmpC (pAmpC) producers (blaCMY positive). On the basis of MLST analysis, the ESBL-KP isolate was identified as sequence type (ST)-133 and four different STs among AmpC-KP isolates, ST-145, ST-4830, ST-4831, and ST-4832, were found to share six of the seven loci constituting a single-locus variant. This is the first study to show K. pneumoniae carrying MDR pAmpC isolated from a racehorse.
DOI： 10.3390/ani10030369
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Goto S., Konnai S., Hirano Y., Kohara J., Okagawa T., Maekawa N., Sajiki Y., Watari K., Minato E., Kobayashi A., Gondaira S., Higuchi H., Koiwa M., Tajima M., Taguchi E., Uemura R., Yamada S., Kaneko M.K., Kato Y., Yamamoto K., Toda M., Suzuki Y., Murata S., Ohashi K.
Frontiers in Veterinary Science 7 12 2020.2
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Frontiers in Veterinary Science
© Copyright © 2020 Goto, Konnai, Hirano, Kohara, Okagawa, Maekawa, Sajiki, Watari, Minato, Kobayashi, Gondaira, Higuchi, Koiwa, Tajima, Taguchi, Uemura, Yamada, Kaneko, Kato, Yamamoto, Toda, Suzuki, Murata and Ohashi. Bovine mycoplasmosis caused by Mycoplasma bovis results in pneumonia and mastitis in cattle. We previously demonstrated that the programmed death 1 (PD-1)/PD-ligand 1 (PD-L1) pathway is involved in immune dysfunction during M. bovis infection and that prostaglandin E2 (PGE2) suppressed immune responses and upregulated PD-L1 expression in Johne's disease, a bacterial infection in cattle. In this study, we investigated the role of PGE2 in immune dysfunction and the relationship between PGE2 and the PD-1/PD-L1 pathway in M. bovis infection. In vitro stimulation with M. bovis upregulated the expressions of PGE2 and PD-L1 presumably via Toll-like receptor 2 in bovine peripheral blood mononuclear cells (PBMCs). PGE2 levels of peripheral blood in infected cattle were significantly increased compared with those in uninfected cattle. Remarkably, plasma PGE2 levels were positively correlated with the proportions of PD-L1+ monocytes in M. bovis-infected cattle. Additionally, plasma PGE2 production in infected cattle was negatively correlated with M. bovis-specific interferon (IFN)-γ production from PBMCs. These results suggest that PGE2 could be one of the inducers of PD-L1 expression and could be involved in immunosuppression during M. bovis infection. In vitro blockade assays using anti-bovine PD-L1 antibody and a cyclooxygenase 2 inhibitor significantly upregulated the M. bovis-specific IFN-γ response. Our study findings might contribute to the development of novel therapeutic strategies for bovine mycoplasmosis that target PGE2 and the PD-1/PD-L1 pathway.
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Clinical efficacy of the combined treatment of anti-PD-L1 rat-bovine chimeric antibody with a COX-2 inhibitor in calves infected with Mycoplasma bovis. Reviewed
Goto, S., Konnai, S., Hirano, Y., Kohara, J., Okagawa, T., Maekawa, N., Sajiki, Y., Watari, K., Minato, E., Kobayashi, A., Gondaira, S., Higuchi, H., Koiwa, M., Tajima, M., Taguchi, E., Ishida, M., Uemura, R., Yamada, S., Kaneko, M., Kato, Y., Yamamoto, K., Toda, M., Suzuki, Y., Murata, S., Ohashi, K.
Japanese Journal of Veterinary Research. 2020.2
Language：English Publishing type：Research paper (scientific journal)
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Neuromedin U suppresses prolactin secretion via dopamine neurons of the arcuate nucleus. Reviewed
Nakahara K, Maruyama K, Ensho T, Mori K, Miyazato M, Kangawa K, Uemura R, Sakoda H, Nakazato M, Murakami N
Biochemical and biophysical research communications 521 ( 2 ) 521 - 526 2020.1
Language：English Publishing type：Research paper (scientific journal)
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Assessment of reproductive and growth performance of pigs on commercial swine farms in southern Kyushu, Japan. Reviewed
Sasaki, Y., Kawabata, T., Nakatake, S., Kohigashi, T., Toya, R., Uemura, R., Sueyoshi, M.
Animal Science Journal 91 ( 1 ) e13492 2020.1
Language：English Publishing type：Research paper (scientific journal)
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Different doses of tannin reflect a double-edged impact on broiler chicken immunity. Reviewed
Ramah A, Yasuda M, Ohashi Y, Urakawa M, Kida T, Yanagita T, Uemura R, Bakry HH, Abdelaleem NM, El-Shewy EA
Veterinary immunology and immunopathology 220 109991 2019.12
Language：English Publishing type：Research paper (scientific journal)
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Ensho T., Maruyama K., Qattali A., Yasuda M., Uemura R., Murakami N., Nakahara K.
Journal of Veterinary Medical Science 81 ( 9 ) 1305 - 1312 2019.9
Language：English Publishing type：Research paper (scientific journal) Publisher：公益社団法人 日本獣医学会
Recently, it has been proposed that neuromedin U (NMU) is "decretin", which suppresses insulin secretion from the pancreas <i>in vitro</i>. Here we examined the possible involvement of NMU in insulin secretion <i>in vivo</i> by comparing the plasma glucose and insulin levels of wild-type mice with those of double knockout (D-KO) of the NMU and neuromedin S (NMS) genes, as NMS binds to the neuromedin U receptor. If NMU is, in fact, "decretin", which inhibits insulin secretion from the pancreas, then NMU-deficient mice might result in higher plasma insulin levels than is the case in wild-type mice, or injection of NMU lead to suppression of plasma insulin level. In this study, we found that the fasting plasma level of insulin was not increased in D-KO mice. Glucose tolerance tests revealed no significant difference in plasma insulin levels between wild-type mice and D-KO mice under non-fasting conditions. After peripheral injection of NMU, plasma glucose and insulin levels did not show any significant changes in either wild-type or D-KO mice. Glucose tolerance testing after 3 weeks of high fat feeding revealed no significant difference in plasma insulin levels during 60 min after glucose injection between wild-type and D-KO mice. These results suggest that even if NMU is a decretin candidate, its physiological involvement in suppression of insulin secretion may be very minor <i>in vivo</i>.
DOI： 10.1292/jvms.19-0320
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Ueno Y., Uemura R., Niwa H., Higuchi T., Sekiguchi S., Sasaki Y., Sueyoshi M.
Journal of Equine Science 30 ( 3 ) 63 - 67 2019.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Equine Science
© 2019 Japanese Society of Equine Science. Equine proliferative enteropathy (EPE) caused by Lawsonia intracellularis is characterized by hypoproteinemia. There are currently no reliable reports that provide a reference value for the total serum protein (TP) concentration to clinically diagnose EPE. The objective of this study was to statistically determine the reference value. Feces and sera of 99 foals with EPE-like clinical signs and of 35 healthy foals were obtained. The samples were used for specific-gene detection of L. intracellularis, TP measurement, and specific-antibody detection against L. intracellularis. Based on these results, the optimal reference value for the TP concentration as a clinical diagnostic index of EPE was found to be ≤ 4.8 g/dl. This clinical diagnostic index will provide an effective approach for diagnosing EPE.
DOI： 10.1294/jes.30.63
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Sukmawinata E., Sato W., Mitoma S., Kanda T., Kusano K., Kambayashi Y., Sato T., Ishikawa Y., Goto Y., Uemura R., Sueyoshi M.
Journal of Equine Science 30 ( 3 ) 47 - 53 2019.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Equine Science
© 2019 Japanese Society of Equine Science. Extended-spectrum β-lactamase-producing Escherichia coli (ESBLEC) have become a major health concern in both human and veterinary medicine. These bacteria could become a critical problem in equine medicine due to the limited number of antimicrobial drugs available. However, there are no previous reports of ESBLEC isolated from horses in Japan. The objectives of this study were to investigate the occurrence of ESBLEC isolated from feces in healthy Thoroughbred racehorses in Japan. Feces samples were collected from 147 healthy Thoroughbred racehorses by equine veterinarians at the Japan Racing Association (103 from Miho Training Center and 44 from Ritto Training Center) between March 2017 and April 2018. Samples were screened for ESBLECs using MacConkey agar supplemented with 1 µg/ml cefotaxime. Detection of ESBL genes was performed by PCR and confirmed by DNA sequencing. Horizontal transmission was demonstrated by conjugation assay. In this study, 24 ESBLECs were isolated from twelve horse feces samples (8.2%). All ESBLECs harbored blaCTX-M-2, and both blaTEM-1 and blaCTX-M-2 were detected in nine isolates (37.5%). ESBLECs showed resistance to all β-lactam antibiotics (100%) tested, followed by trimethoprim (66.7%), streptomycin (62.5%), tetracycline (25.0%), and oxytetracycline (25.0%). Horizontal transmission was successfully demonstrated by conjugation assay in eight of 13 isolates, and blaCTX-M-2 was detected by PCR in all transconjugants. This study showed that racehorses in Japan are potential reservoirs of ESBLECs.
DOI： 10.1294/jes.30.47
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Bovine Endocarditis Associated with Mycoplasma bovis Reviewed
Kanda T., Tanaka S., Suwanruengsri M., Sukmawinata E., Uemura R., Yamaguchi R., Sueyoshi M.
Journal of Comparative Pathology 171 53 - 58 2019.8
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Comparative Pathology
© 2019 Elsevier Ltd Mycoplasma bovis is a microorganism associated with pneumonia, mastitis, arthritis and otitis media of cattle; however, there are no reports of this organism causing bovine endocarditis. Five adult cattle with endocarditis characterized by caseated lesions (diameter 5–12 cm) of the endocardial surface of the left atrium, but without lesions in heart valves or affecting the right side of the heart, were identified in slaughterhouses in Japan. M. bovis was successfully isolated from the lesions and M. bovis antigen was detected immunohistochemically within the lesions. The results suggest that the lesions may have been associated with M. bovis alone. To our knowledge, this is the first demonstration of bovine endocarditis associated with M. bovis.
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HIROKI Hayate, KUROYANAGI Aakira, KOBAYASHI Ikuo, UEMURA Ryoko, NUKAZAWA Kei, SUZUKI Yoshihiro
Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research) 74 ( 7 ) III_231 - III_238 2018.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society of Civil Engineers
Livestock that have been administered an antibiotic treatment are highly likely to carry antibiotic-resistant bacteria. Because small animals (mice) live alongside livestock in stock farms and consume their feed and feces, it is possible that these antibiotic-resistant bacteria are transmitted to them. In this study, cattle and mice feces collected from the Sumiyoshi Livestock Science Station, University of Miyazaki, Japan, were tested for the presence of antibiotic-resistant <i>Escherichica coli</i>. Susceptibility of the <i>E. coli</i> isolates from the fecal samples to 11 antibiotics was evaluated using the disk diffusion method. The resistance profiles of <i>E. coli</i> isolates to various antibiotics were evaluated and compared between cattle and mice feces. Antibiotic-resistant <i>E. coli</i> were detected in 50% of cattle (5/10 animals) and 41% of mice (7/17 animals). In addition, all isolates from both cattle and mice feces had high detection rates of antibiotic resistance for ampicillin and tetracycline, and one isolate from the mice feces contained multidrug-resistant <i>E. coli</i>. Examination of the distribution of mice with antibiotic-resistant <i>E. coli</i> suggested that these bacteria are transmitted by mice and can spread to the stock farm.
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Sukmawinata E., Sato W., Uemura R., Sueyoshi M.
Journal of Equine Veterinary Science 63 51 - 54 2018.4
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Equine Veterinary Science
© 2018 Elsevier Inc. Antimicrobial resistant (AMR) Enterococci are an emerging problem for human and animal health. Few studies have focused on the detection of AMR in foals; therefore, we conducted this study to investigate the occurrence of AMR Enterococci in foal feces in Japan. A total of 72 fecal samples from healthy foals were collected from seven horse stables, in Hokkaido Prefecture, from June 2015 to January 2016. Enterococci were isolated and identified using EF Agar Base selective media, and the species confirmed using MALDI Biotyper systems. The minimum inhibitory concentration of nine antimicrobial agents for the isolates was determined based on the Clinical Laboratory Standard Institute guidelines. A total of 183 isolates of Enterococci were identified as Enterococcus faecium 54.1% (99/183), Enterococcus faecalis 16.4% (30/183), and other species 29.5% (54/183). The highest occurrence of E. faecium antibiotic resistant was to erythromycin at 55.6% (55/99), followed by enrofloxacin, kanamycin, and oxytetracycline, at 30.3%, 7.1%, and 4.0%, respectively. In contrast, E. faecalis isolates showed higher resistant to oxytetracycline 76.7%, kanamycin 46.7%, gentamycin 30.0%, chloramphenicol 16.7%, lincomycin 30.0%, and tylosin 30.0%. E. faecium highly resistant to erythromycin and enrofloxacin but lowly resistant to gentamycin and tylosin, and E. faecalis was highly resistant to kanamycin, oxytetracycline, and lincomycin. Antimicrobial resistant E. faecalis (30.0%) and E. faecium (4.0%) isolates show multidrug resistant to at least three different classes of antibiotics. Our study indicated that foal feces may act as a source of AMR genes for Enterococci that may be transmitted to other animals, humans, and the environment.
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Effects of zinc supplementation on Shiga toxin 2e-producing Escherichia coli in vitro. Reviewed
Uemura, R., Katsuge T., Sasaki, Y., Goto, S., Sueyoshi, M.
Journal of Veterinary Medical Science 79 ( 10 ) 1637 - 1643 2017.10
Language：English Publishing type：Research paper (scientific journal) Publisher：公益社団法人 日本獣医学会
Swine edema disease is caused by Shiga toxin (Stx) 2e–producing <i>Escherichia coli</i> (STEC). Addition of highly concentrated zinc formulations to feed has been used to treat and prevent the disease, but the mechanism of the beneficial effect is unknown. The purpose of the present study was to investigate the effects of highly concentrated zinc formulations on bacterial growth, hemolysin production, and an Stx2e release by STEC <i>in vitro</i>. STEC strain MVH269 isolated from a piglet with edema disease was cultured with zinc oxide (ZnO) or with zinc carbonate (ZnCO<sub>3</sub>), each at up to 3,000 ppm. There was no effect of zinc addition on bacterial growth. Nonetheless, the cytotoxic activity of Stx2e released into the supernatant was significantly attenuated in the zinc-supplemented media compared to that in the control, with the 50% cytotoxic dose values of 163.2 ± 12.7, 211.6 ± 33.1 and 659.9 ± 84.2 after 24 hr of growth in the presence of ZnO, ZnCO<sub>3</sub>, or no supplemental zinc, respectively. The hemolytic zones around colonies grown on sheep blood agar supplemented with zinc were significantly smaller than those of colonies grown on control agar. Similarly, hemoglobin absorbance after exposure to the supernatants of STEC cultures incubated in sheep blood broth supplemented with zinc was significantly lower than that resulting from exposure to the control supernatant. These <i>in vitro</i> findings indicated that zinc formulations directly impair the factors associated with the virulence of STEC, suggesting a mechanism by which zinc supplementation prevents swine edema disease.
DOI： 10.1292/jvms.16-0471
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Sabike, I., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Farid, A., Goto, Y., Horii, Y., Yamazaki, W.
Food Control 74 107 - 111 2017.4
Language：English Publishing type：Research paper (scientific journal) Publisher：Food Control
© 2016 Elsevier Ltd In a two-year survey of the 24 Japanese broiler chicken flocks at 9 farms from 2013 to 2014, C. jejuni/C. coli prevalence was assessed in a total of 131 slaughtered broiler chicken cecal samples by conventional culture methods and loop-mediated isothermal amplification (LAMP) assay. While 93 samples were C. jejuni/C. coli-negative, 38 (29.0%) showed Campylobacter loads of between 6.4 and 9.0 log CFU/g of ceca in conventional culture methods. The performance of LAMP assay was 100% accurate in terms of diagnostic sensitivity (38/38), specificity (93/93). Furthermore, LAMP assay enabled direct screening of C. jejuni and C. coli in cecal samples from broiler chicken chickens as rapid and cost-effective detection within 90 min and less than 1 US dollar, which can help monitor release of Campylobacter-contaminated chicken into the food chain, thereby reducing the incidence and public health risk of campylobacteriosis. Seasonal changes in C. jejuni and C. coli prevalence in broiler chicken ceca were significantly correlated with the frequency of food poisoning incidents caused by these bacteria in Japan.
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Sabike, I., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Okabayashi, T., Goto, Y., Yamazaki, W.
Frontiers in Microbiology 7 1582 2016.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Frontiers in Microbiology
© 2016 Sabike, Uemura, Kirino, Mekata, Sekiguchi, Okabayashi, Goto and Yamazaki. Rapid identification of Campylobacter-positive flocks before slaughter, following freezing and heat treatment for the Campylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. We evaluated a loop-mediated isothermal amplification (LAMP) assay for the direct screening of naturally contaminated chicken cloacal swabs for C. jejuni/C. coli to compare this assay with conventional qu antitative culture methods. In a comparison study of 165 broilers, the LAMP assay showed 82.8% (48/58 by conventional culture) sensitivity, 100% (107/107) specificity, 100% (48/48) positive predictive value (PPV), and 91.5% (107/117) negative predictive value (NPV). In a comparison of 55 flocks, LAMP showed 90.5% (19/21) sensitivity, 100% (34/34) specificity, 100% (19/19) PPV, and 94.4% (34/36) NPV. In the cumulative total of 28 farm-level comparisons, LAMP showed 100% (12/12) sensitivity, 100% (16/16) specificity, 100% (12/12) PPV, and 100% (16/16) NPV. The LAMP assay required less than 90 min from the arrival of the fecal samples to final results in the laboratory. This suggests that the LAMP assay will facilitate the identification of C. jejuni/C. coli-positive broiler flocks at the farm level or in slaughterhouses before slaughtering, which would make it an effective tool in preventing the spread of Campylobacter contamination.
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Body surface temperature of suckling piglets measured by infrared thermography and its association with body weight change. Reviewed
Sasaki, Y., Furusho, K., Ushijima, R., Tokunaga, T., Uemura, R., Sueyoshi, M.
Japan Agricultural Research Quarterly 50 ( 4 ) 361 - 368 2016.8
Language：English Publishing type：Research paper (scientific journal)
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2010年に宮崎で発生した口蹄疫の防疫措置終了後に農場内に留置された家畜排泄物のウイルス残存性調査. Reviewed
國保健浩，真瀬昌司，亀山健一郎，池口厚男，田中康男，吉田和生，山田俊治，大橋誠一，深井克彦，森岡一樹，小野里洋行，堀井 洋一郎，西脇亜也，飛佐学，佐伯雄一，井戸田幸子，上村涼子，末吉益雄，川嶌健司.
産業動物臨床獣医学雑誌 7 ( 1 ) 1 - 8 2016.8
Language：Japanese Publishing type：Research paper (scientific journal)
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An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis. Reviewed
Higa, Y., Uemura, R., Yamazaki, W., Goto, S., Goto, Y., Sueyoshi, M.
The Journal of Veterinary Medical Science 78 ( 8 ) 1343 - 1346 2016.8
Language：English Publishing type：Research paper (scientific journal)
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Yamazaki, W., Uemura, R., Sekiguchi, S., Dong, J., Watanabe, S., Kirino, Y., Mekata, H., Nonaka, N., Norimine, J., Sueyoshi, M., Goto, Y., Horii, Y., Kurogi, M., Yoshino, S., Misawa, N.
Journal of Applied Microbiology 120 ( 6 ) 1711 - 1722 2016.6
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Applied Microbiology
© 2016 The Society for Applied Microbiology. Aim: To elucidate the distribution and circulation dynamics of Campylobacter and Salmonella in Japanese chicken broiler flocks. Methods and Results: A 2-year investigation of the distribution of Campylobacter and Salmonella was conducted in 25 broiler flocks at nine farms in Japan from 2013 to 2014. Campylobacter and Salmonella tested positive in 11 (44·0%) and 24 (96·0%) broiler flocks respectively. One hundred and ninety-five Campylobacter and 184 Salmonella isolates were characterized into 12 Campylobacter (including two novel genotypes) and three Salmonella MLST genotypes. Only Salmonella isolation between caecal and environmental samples were significantly correlated. Further, one litter sample tested positive for Salmonella before new chicks were introduced. The Campylobacter strains rapidly lost culturability within 2-18 days; in contrast, the Salmonella strains survived from 64-211 days in artificially inoculated water samples. Conclusion: No persistent circulation-mediated Campylobacter contamination was observed. In contrast, circulation of Salmonella in broiler houses was seen, apparently due to the litter excreted from broiler flocks, as well as Salmonella-contaminated water and feed. Significance and Impact of the Study: This paper provides the distribution, genotypic data and circulation dynamics of Campylobacter and Salmonella as recently observed in Japanese chicken broiler farms.
DOI： 10.1111/jam.13141
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SASAKI Yosuke, SEKIGUCHI Satoshi, UEMURA Ryoko, SUEYOSHI Masuo
Journal of Veterinary Medical Science 78 ( 2 ) 333 - 335 2016
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
This study compared the reproductive and growth performances of pigs before and after depopulation and restocking after a foot-and-mouth disease outbreak in Japan. Data for the time period before and after depopulation and restocking were obtained from three farrow-to-finish farms. As a result of depopulation and restocking, hygiene levels were improved, and common infectious diseases, such as porcine reproductive and respiratory syndrome and Aujeszky's disease, remained undetected on the farms. Compared with before depopulation, reproductive and growth performances improved after depopulation; the number of total pigs born was higher, the postweaning mortality rate was lower, and the age at slaughter was lower (<i>P</i><0.05). In summary, depopulation and restocking improved the reproductive and growth performances of pigs.
DOI： 10.1292/jvms.15-0013
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口蹄疫等の防疫用移動式レンダリング装置から排出される加熱破砕物の充填・運搬手法の検討. Reviewed
田中康男，八木行雄，末吉益雄，勝田 賢，宮崎綾子，鈴木亨，池口厚男，會田紀雄，石田三佳，中久保亮，上村涼子，橋本仁康，弓削正昭，大崎慎人，犬丸茂樹，荻野暁史。山下恭広.
家畜衛生学雑誌 40 ( 4 ) 203 - 213 2015.4
Language：Japanese Publishing type：Research paper (scientific journal)
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Sasaki Y., Uemura R., Sekiguchi S., Takahashi T., Fujii Y., Sueyoshi M.
British Poultry Science 55 ( 6 ) 737 - 744 2014.11
Language：English Publishing type：Research paper (scientific journal) Publisher：British Poultry Science
© 2014, © 2014 British Poultry Science Ltd. Abstract: 1. The present study determined descriptive values of the main production measurements of flocks and assessed the relationship between these measurements and related management factors in Japanese commercial broiler farms. 2. The data set included 5060 flock records from 183 farms. The production index was calculated as follows: liveability × average daily gain/feed conversion ratio × 10. Management factors included in the analysis were broiler breeder age, the time interval between successive flocks, the season of placement and stocking density. 3. The mean (±SD) production index was 283.9 ± 28.83. Management factors significantly associated with a decreased production index were low broiler breeder age, flocks placed in summer and high stocking density (P < 0.05). 4. In regard to an interaction for the production index, flocks with high stocking density had a lower production index than those with low stocking density in flocks with a low broiler breeder age (P < 0.05). In summer, flocks with a short time interval between successive flocks had a lower production index than those with an intermediate or long time interval (P < 0.05). 5. The present study identified factors related to flock performance. The knowledge obtained from this analysis will contribute to improve flock performance by optimising management.
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口蹄疫等の防疫における移動式レンダリング装置活用のための技術開発. Reviewed
八木行雄, 末吉益雄, 勝田賢, 宮崎綾子, 鈴木亨, 田中康男, 池口厚男, 會田紀雄, 石田三佳, 中久保亮, 上村涼子, 橋本仁康, 弓削正昭, 大崎慎人, 犬丸茂樹.
家畜衛生学雑誌 39 149 - 156 2014
Language：Japanese Publishing type：Research paper (scientific journal)
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An assessment of reproductive and lifetime performances of Kagoshima Berkshire gilts and sows. Reviewed
Sasaki, Y., Tokunaga, T., Uemura, R., and Sueyoshi, M.
Animal Science Journal 59 ( 10 ) 601 - 606 2013.10
Language：English Publishing type：Research paper (scientific journal)
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口蹄疫防疫措置の終了した発生農場に留置された家畜排泄物の状態
１１） 井戸田幸子, 飛佐 学, 上村涼子, 堀井洋一郎, 末吉益雄, 石井康之, 西脇亜也, 山本直之
日本草地学会誌 58 277 - 279 2013.1
Language：Japanese Publishing type：Research paper (scientific journal)
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The investigation of respiratory infectious mycoplasmas of cattle in Japan. Reviewed
R. Uemura, K. Nakamori, M. Sueyoshi
J. Jpn. Vet. Med. Assoc. 62 ( 11 ) 871 - 875 2012.11
Language：Japanese Publishing type：Research paper (scientific journal)
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Yamazaki W., Kumeda Y., Uemura R., Misawa N.
Food Microbiology 28 ( 6 ) 1238 - 1241 2011.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Food Microbiology
We investigated the efficacy of a loop-mediated isothermal amplification (LAMP) assay for rapid screening of seafood samples naturally contaminated with Vibrio parahaemolyticus. A total of 171 seafood samples enriched in alkaline peptone water (APW) were assessed by LAMP assay and conventional culture methods, which consist of a combination of APW enrichment culture and plating onto CHROMagar Vibrio and TCBS agars. Compared with V. parahaemolyticus isolation using the conventional culture test, LAMP results showed 100% (30/30) and 90.8% (128/141) sensitivity and specificity, respectively. The conventional culture test required more than 3 days to isolate and identify V. parahaemolyticus in the APW enrichment culture. In contrast, the LAMP assay was markedly faster, requiring less than 60 min from the beginning of DNA extraction to final detection of V. parahaemolyticus. In total, the LAMP assay required 17-19 h from the beginning of enrichment culture to final determination. This is the first report of the LAMP assay for rapid screening of seafood samples naturally contaminated by V. parahaemolyticus. © 2011 Elsevier Ltd.
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Uemura R., Sueyoshi M., Nagatomo H.
Journal of Veterinary Medical Science 72 ( 12 ) 1661 - 1663 2010.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Veterinary Medical Science
We examined the susceptibility in vitro of 99 isolates of Mycoplasma (M. bovirhinis, M. bovis, M. alkalescens and M. bovoculi) and one of Acholeplasma laidlawii collected in 2008 and 2009 from Japanese homebred cattle and cows imported from Australia to 13 antimicrobial agents. Mycoplasma species isolated from homebred cattle were susceptible to spectinomycin and fluoroquinolones but resistant to macrolides, tetracyclines, thiamphenicol and flumequine. Kanamycin, lincomycin and chloramphenicol maintained intermediate effectiveness against these species. The isolates from the imported cows maintained sufficient susceptibility to all antimicrobials examined except for erythromycin and thiamphenicol. These results showed that four species of Mycoplasma isolated from Japanese homebred cattle had obviously higher drug resistance compared with previous reports, even within this decade.
DOI： 10.1292/jvms.10-0165
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An atypical porcine proliferative enteropathy of malgrowth piglets. Reviewed
M. Sueyoshi, A. Arikawa, R. Uemura K. Nakamori and H. Nagatomo
Journal of Veterinary Medical Science 72 ( 11 ) 1483 - 1485 2010.11
Language：English Publishing type：Research paper (scientific journal)
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Secondary Mass-Outbreak of Enterotoxaemia with Escherichia coli on a Pig Farm
YUJI Hiroyuki, SUEYOSHI Masuo, NAGATOMO Hiroshi, UEMURA Ryoko, TAURA Yasuho
Journal of the Japan Veterinary Medical Association 62 ( 3 ) 211 - 214 2009.3
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Veterinary Medical Association
The acute death of postweaning piglets occurred persistently from October 1997 until June 2005 on a pig farm. The appearance of the affected piglets showed remarkable palpebral edema in the first peak, and diarrhea was confirmed as a prodromal symptom in the latter peak. The piglets were diagnosed as having enterotoxaemia with <i>Escherichia coli</i>. A total of approximately 12,000 piglets died in this case.
DOI： 10.12935/jvma.62.211
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Uemura R., Sueyoshi M., Taura Y., Nagatomo H.
Journal of Veterinary Medical Science 66 ( 8 ) 899 - 903 2004.8
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Veterinary Medical Science
Edema disease (ED) of pigs is an enterotoxaemic disease caused by enterotoxaemic Escherichia coli (ETEEC) infection. Antimicrobial therapy for pigs with ED is controversial because it may induce death of sickish piglets. In this study, we investigated the effects in vitro of 7 antimicrobial agents, ampicillin, gentamicin, colistin, bicozamycin, fosfomycin, sulfamethoxazole- trimethoprim and enrofloxacin, on the release and production of shiga toxin (Stx) 2e by ETEEC strains. We found that more Stx 2e accumulated in the bacterial cells than was released into supernatant. Associated with inhibition of cell wall synthesis, the exposure to ampicillin or fosfomycin increased the release of Stx 2e. The production levels of Stx 2e in all antimicrobial-treated cultures were equal to the level in the control or less than in the control. These results suggest that cell wall synthesis inhibitors, such as ampicillin and fosfomycin, may change for the worse in the signs in ETEEC infectious pigs. On the other hand, gentamicin, colistin, bicozamycin and enrofloxac in may be useful for the treatment of pigs with ED.
DOI： 10.1292/jvms.66.899
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Protracted Mass Occurrences of Sudden Death among Piglets with Escherichia coli Enterotoxaemia Reviewed
UEMURA Ryoko, SUEYOSHI Masuo, TAURA Yasuho, NAGATOMO Hiroshi
Journal of the Japan Veterinary Medical Association 57 ( 4 ) 231 - 234 2004.4
Language：Japanese Publishing type：Research paper (scientific journal)
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Uemura R., Sueyoshi M., Nagayoshi M., Nagatomo H.
Microbiology and Immunology 47 ( 1 ) 57 - 61 2003.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Microbiology and Immunology
Fifty-seven Shiga toxin-producing Escherichia coli (STEC) strains isolated from pigs with edema disease (ED) from 1997 to 2001 in Japan were examined for antimicrobial susceptibilities. The susceptibilities were compared with those of E. coli ATCC 23546 isolated from pig with ED in the 1950's. Consequently, the isolated STECs showed high susceptibility to peptides and bicozamycin in a way similar to the reference strain. On the other hand, the STECs showed low susceptibility to beta-lactams, tetracyclines, novobiocin, fosfomycin, trimethoprim, and old quinolones. It became clear that the susceptibilities of the isolated STECs had diminished in regard to antimicrobials.
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Nagatomo H., Takegahara Y., Sonoda T., Yamaguchi A., Uemura R., Hagiwara S., Sueyoshi M.
Veterinary Microbiology 82 ( 3 ) 223 - 232 2001.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Veterinary Microbiology
A comparison of the persistence of mycoplasmas in animals was carried out. When inoculated into liquid media, strains of Mycoplasma bovis, M. arginini, Acholeplasma laidlawii, and A. axanthum persisted for 59-185 days post-inoculation. The survival periods were not significantly influenced by temperature (4, 30, 37°C, and room temperature). The survival periods for M. bovigenitalium, M. gallisepticum, M. bovirhinis, and M. gateae ranged from < 7 to 185 days depending on medium components and temperature. Further, it was determined that strains of M. bovigenitalium, M. bovis, M. bovirhinis, M. arginini, and A. laidlawii persisted in a dry paper disc for at most 28, 126, 154, 56 and over > 168 days at 4°C, respectively. At 4°C, strains of M. gallisepticum, M. columborale, M. edwardii, M. felis, and M. gateae survived for at most 28, 21, 42, 28, 28 and 70 days, respectively. At 30°C, strains of M. bovis, M. bovirhinis, M. arginini, A. laidlawii, and M. gallisepticum persisted for at most 28, 84, 56, > 168 and 14 days, respectively, but strains of M. gallisepticum, M. columborale, M. edwardii, M. felis, M. gateae, and U. diversum did not survive for more than 14 days. In an outdoor environment, strains of M. bovirhinis and A. laidlawii survived for at most 28 and 14 days, respectively. Finally, it was found that 14 isolates of M. gallisepticum persisted for periods similar to those of the reference strains. The results under dry conditions at a variety of temperatures presented contribute to understanding the epizootiology of mycoplasmal infections in the field. © 2001 Elsevier Science B.V. All rights reserved.
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Effect of Bicozamycin on the Eradication of Shiga Toxin-Producing Escherichia coli in Calves Reviewed
Misawa Naoaki, Sueyoshi Masuo, Uemura Ryoko, KAKEMIZU Yoshihiro, KAWASHIMA Kumiko, NAGATOMO Hiroshi, KONDO Fusao, MURAKAMI Takayuki, TAKAHASHI Yoshihiro
Microbiology and immunology 44 ( 11 ) 891 - 896 2000.11
Language：English Publishing type：Research paper (scientific journal) Publisher：Center for Academic Pub. Japan