Papers - UEMURA Ryoko
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A highly sensitive method for detecting African swine fever virus in oral fluids from naturally infected pigs in Northern Vietnam Reviewed International coauthorship
Thi Ngan Mai, Thi Huong Giang Tran, Van Hieu Dong, Van Phan Le, Thi My Le Huynh, Tran Anh Dao Bui, Ryoko Uemura, Yasuko Yamazaki, Thi Lan Nguyen & Wataru Yamazaki
Scientific Reports 15 27855 2025.7
Language：English Publishing type：Research paper (scientific journal)
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Comprehensive serotyping of Mannheimia haemolytica by a PCR system using the diversity of capsule biosynthesis genes. Reviewed
Iguchi A, Ueno Y, Hoshinoo K, Okuno M, Uemura R, You G, Ogura Y, Takamatsu D
Scientific reports 15 ( 1 ) 11970 2025.4
Language：English Publishing type：Research paper (scientific journal)
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Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes Reviewed
Nishi K., Gondaira S., Hirano Y., Ohashi M., Sato A., Matsuda K., Iwasaki T., Kanda T., Uemura R., Higuchi H.
Veterinary research 56 ( 1 ) 22 2025.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Veterinary research
Mycoplasma pneumonia, caused by Mycoplasma bovis (Mycoplasmopsis bovis; M. bovis), is linked with severe inflammatory reactions in the lungs and can be challenging to treat with antibiotics. Biofilms play a significant role in bacterial persistence and contribute to the development of chronic lesions. A recent study has shown that polymicrobial interactions between species are an important factor in biofilm formation, yet the precise mechanism of biofilm formation in M. bovis remains unknown. By assuming multiple pathogen infections in the bovine respiratory disease complex (BRDC), this study examined the characterisation of the polymicrobial relationship between M. bovis and Trueperella pyogenes (T. pyogenes) during biofilm formation. Autopsies were performed on four Holstein calves (two chronic Mycoplasma pneumonia calves and two control calves). Bacterium-like aggregation structures (> 10 μm), which were assumed to be biofilms of M. bovis in vivo, were observed adhering to the cilia in calves with Mycoplasma pneumonia. M. bovis released an extracellular matrix to connect with neighbouring bacteria and form a mature biofilm on the plate. Biofilm formation in the co-culture of M. bovis and T. pyogenes (strain T1: 1 × 105 and 1 × 106 CFU/well) significantly increased (p < 0.05 and p < 0.01; 64.1% and 64.8% increase) compared to that in a single culture of these bacteria. Furthermore, some large aggregates (> 40 μm), composed of M. bovis and T. pyogenes, were observed. The morphological characteristics of this biofilm were similar to those observed in vivo compared to a single culture. In conclusion, the polymicrobial interaction between M. bovis and T. pyogenes induces biofilm formation, which is associated with increased resistance to antimicrobial agents, and this exacerbates the progression of chronic Mycoplasma pneumonia.
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Efficacy of Treating Bacterial Bioaerosols with Weakly Acidic Hypochlorous Water: A Simulation Chamber Study Reviewed
Norkaew S, Narikawa S, Nagashima U, Uemura R, Noda J
Heliyon 2024.1
Language：English Publishing type：Research paper (scientific journal)
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A case of urachal abscess resection via colpotomy in a cow Reviewed
SATO Reiichiro, SATO Yukiko, FUKE Naoyuki, UEMURA Ryoko, STEINER Adrian
Journal of Veterinary Medical Science 86 ( 11 ) 1156 - 1161 2024
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
This study describes a novel surgical method to remove urinary bladder-related urachal abscesses in a cow. Traditional methods, such as the midline or paramedian approach in calves and the preinguinal approach, can be challenging in adult cattle, especially for lesions situated near the pelvic cavity. In this case, a 4-year-old cow had a urachal abscess attached to the bladder apex, making it difficult to approach via abdominal wall incision. Colpotomy and pulling the abscess through a vaginal wall incision allowed for complete exteriorization of the lesion and comfortable accessibility of the abscess and the resection site. This report suggests that colpotomy is an effective new route in adult cattle for accessing urachal abscesses not accessible via traditional surgical approaches.
DOI： 10.1292/jvms.24-0256
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Sukmawinata E., Uemura R., Nishiki I., Yoshida T., Sueyoshi M., Kikuchi T.
Veterinarski Glasnik 78 ( 1 ) 81 - 87 2024
Publishing type：Research paper (scientific journal) Publisher：Veterinarski Glasnik
Plasmids play an important role in the spread of antimicrobial resistance genes (ARGs) in bacteria. This study reports the complete sequence of the IncX3 plasmid identified in Escherichia coli isolated from faeces of a Japanese racehorse. Sequence analysis revealed that this plasmid harbours the blaSHV-12 gene, which encodes an extended spectrum β-lactamase, and the quinolone resistance gene qnrS1. The IncX3 plasmids carrying ARGs have been previously identified in bacteria isolated from humans, animals and a variety of environments. This is the first report of the complete sequence of the IncX3 plasmid carrying blaSHV-12 and qnrS1 genes from a Japanese racehorse, which provides insights into understanding the spread and mechanism of antimicrobial resistance particularly in Japanese racehorses.
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Shitamori F., Uemura R., Kanda T., Sueyoshi M.
Open Veterinary Journal 12 ( 6 ) 782 - 786 2022.11
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Open Veterinary Journal
Background: Mycoplasma bovis causes various diseases such as bronchopneumonia, otitis media, arthritis, and mastitis in cattle. Mycoplasma bovis is often isolated from the deep pharynges of healthy cattle and is generally considered not to cause clinical symptoms while in the upper respiratory tract. In mycoplasma infections, adhesion to the host cells is a crucial step. In recent years, five new adhesins, NOX, α-enolase, TrmFO, P27, and VpmaX, have been reported in M. bovis strains from pneumonia cases. However, the presence of these adhesins in wild isolates has not been established. Aim: This study aimed to investigate the presence of these adhesin genes in wild isolates isolated from cattle nasal cavities and lesion sites (pneumonia, otitis media, arthritis, and mastitis) in various regions in Japan and clarify the relationship between adhesion and the symptoms caused by M. bovis infection. Methods: A total of 141 M. bovis wild isolates isolated from nasal cavities (healthy or sick cattle), lungs with pneumonia, ears with otitis media, joint fluids of arthritic animals, and milk of mastitic animals. Mycoplasma bovis type strain PG45 was also used. Specific polymerase chain reaction reactions were performed to detect nox, α-enolase, trmFO, P27, and vpmaX, which are adhesins of M. bovis. Results: This study reports 139 M. bovis wild isolates were positive for nox, α-enolase, trmFO, P27, and vpmaX, while two isolates each lacked α-enolase or P27 genes. Mycoplasma bovis PG45 also had all five adherens genes. Conclusion: Almost all M. bovis wild isolates possessed all nox, α-enolase, trmFO, P27, and vpmaX genes regardless of the lesion site or region of origin. This means no relationship was found between the presence of the five adhesins and lesion sites in M. bovis and M. bovis isolated from the nasal cavities of asymptomatic cattle have the same numbers and types of adhesins as isolates from symptomatic lesion sites (pneumonia, otitis media, arthritis, and mastitis). This suggests that not only M. bovis isolates from pulmonary lesions, but also M. bovis existing in the nasal cavity has the potential to causes symptoms in the host.
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Effects of Probiotics on Growth and Immunity of Piglets. Reviewed
Azizi AFN, Uemura R, Omori M, Sueyoshi M, Yasuda M
Animals : an open access journal from MDPI 12 ( 14 ) 2022.7
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
DOI： 10.3390/ani12141786
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Mathurot Suwanruengsri Ryoko Uemura Uda Zahli Izzati Takuya Kanda Naoyuki Fuke Masahiro Yasuda Takuya Hirai Ryoji Yamaguchi
Thai J Vet Med. 52 ( 1 ) 33 - 44 2022.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Comparative Pathology
Mycoplasma hyopneumoniae (Mhp) is the primary pathogen of porcine enzootic pneumonia (PEP). Consolidated lung tissue from the cranioventral lung lobes of 15 pigs with PEP was collected for quantitative polymerase chain reaction, histopathology and immunohistochemistry. Histopathology revealed the co-existence of bronchial-associated lymphoid tissue hyperplasia with intra-alveolar neutrophils and macrophage infiltration in lesions of suppurative bronchopneumonia. Immunolabelling of infiltrated macrophages with CD163/CD204 indicated the presence of M2-polarized macrophages. Mhp antigen was detected on respiratory epithelial cells and in phagocytosed neutrophils. The intensity of Mhp immunolabelling and number of CD163/CD204-positive macrophages were correlated with the Mhp load in lung tissue (r = 0.87, 0.56, P <0.05). IL-8 immunolabelling was mainly found in neutrophils and correlated with Mhp load, Mhp immunolabelling and histological lesion score (r = 0.70, 0.66, 0.64, P <0.05), respectively. Apoptosis was seen in intra-alveolar cells and was correlated with Mhp load (r = 0.62, P <0.05). It is postulated that IL-8 attracts neutrophils to the lesions, while M2-polarized macrophages are a major source of IL-10 and promote a Th2-type immune response.
DOI： 10.14456/tjvm.2022.4
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Optimizing antimicrobial use by improving medication adherence among pig producers Reviewed
Toya, Ryohei; Sasaki, Yosuke; Uemura, Ryoko; Sueyoshi, Masuo
Animal Science Journal 2022
Language：English Publishing type：Research paper (scientific journal)
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Antibiotic-resistant Escherichia coli isolated from dairy cows and their surrounding environment on a livestock farm practicing prudent antimicrobial use Reviewed
Yoshihiro Suzuki*, Hayate Hiroki, Hui Xie, Masateru Nishiyama, Shinsuke H. Sakamoto, Ryoko Uemura, Kei Nukazawa, Yoshitoshi Ogura, Toru Watanabe, and Ikuo Kobayashi
International Journal of Hygiene and Environmental Health 2022
Language：English Publishing type：Research paper (scientific journal)
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Eimeria pragensis infection alters the gut microenvironment to favor extrinsic shiga toxin-producing Escherichia coli O157:H7 colonization in mice Reviewed
Ryoko Uemura, Miki Kawakado, Masuo Sueyoshi, Nariaki Nonaka and Yoichiro Horii
Parasitology International 2022
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
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Interferon gamma, lipopolysaccharide, and modified-live viral vaccines stimulation alter the mRNA expression of tumor necrosis factor α, inducible nitric oxide synthase, and interferon β in bovine alveolar macrophages Reviewed
Shingo Ishikawa, Masataka Miyazawa, Chiho Tanaka, Ryoma Uesawa, Juri Nishizawa, Ryoko Uemura, Ikuo Kobayashi, Seiji Hobo
Veterinary Immunology and Immunopathology 2022
Language：English Publishing type：Research paper (scientific journal)
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Protective effects of probiotics against tannin-induced immunosuppression in broiler chickens Reviewed
RAMAH Amany, YASUDA Masahiro, OHASHI Yuki, IMATAKE Shoichiro, IMAIZUMI Noriko, KIDA Tetsuo, YANAGITA Tenya, UEMURA Ryoko, BAAKHTARI Mahmoud, BAKRY Hatem H., ABDELALEEM Nabila M., EL-SHEWY Elham A.
Bifidobacteria and Microflora advpub ( 0 ) 2022
Language：English Publishing type：Research paper (scientific journal) Publisher：BMFH出版会
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Production of granulomas in <i>Mycoplasma bovis</i> infection associated with meningitis-meningoencephalitis, endocarditis, and pneumonia in cattle. Reviewed
Suwanruengsri M, Uemura R, Kanda T, Fuke N, Nueangphuet P, Pornthummawat A, Yasuda M, Hirai T, Yamaguchi R
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 10406387211053254 2021.11
Language：English Publishing type：Research paper (scientific journal)
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Nueangphuet P., Suwanruengsri M., Fuke N., Uemura R., Hirai T., Yamaguchi R.
Journal of Comparative Pathology 189 31 - 44 2021.11
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Comparative Pathology
Mycoplasma hyopneumoniae (Mhp) is the primary pathogen of porcine enzootic pneumonia (PEP). Consolidated lung tissue from the cranioventral lung lobes of 15 pigs with PEP was collected for quantitative polymerase chain reaction, histopathology and immunohistochemistry. Histopathology revealed the co-existence of bronchial-associated lymphoid tissue hyperplasia with intra-alveolar neutrophils and macrophage infiltration in lesions of suppurative bronchopneumonia. Immunolabelling of infiltrated macrophages with CD163/CD204 indicated the presence of M2-polarized macrophages. Mhp antigen was detected on respiratory epithelial cells and in phagocytosed neutrophils. The intensity of Mhp immunolabelling and number of CD163/CD204-positive macrophages were correlated with the Mhp load in lung tissue (r = 0.87, 0.56, P <0.05). IL-8 immunolabelling was mainly found in neutrophils and correlated with Mhp load, Mhp immunolabelling and histological lesion score (r = 0.70, 0.66, 0.64, P <0.05), respectively. Apoptosis was seen in intra-alveolar cells and was correlated with Mhp load (r = 0.62, P <0.05). It is postulated that IL-8 attracts neutrophils to the lesions, while M2-polarized macrophages are a major source of IL-10 and promote a Th2-type immune response.
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Mycoplasma bovis may travel along the eustachian tube to cause meningitis in Japanese black cattle Reviewed
Suwanruengsri M., Uemura R., Izzati U.Z., Kanda T., Fuke N., Yasuda M., Hirai T., Yamaguchi R.
Journal of Comparative Pathology 188 13 - 20 2021.10
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of Comparative Pathology
Mycoplasma bovis (M. bovis) is a common inhabitant of the upper and lower respiratory tracts of cattle and is considered to be the main aetiological agent of otitis media in calves. The eustachian tube appears to be the most common portal for pathogens to enter the middle ear. We investigated the transmission route of M. bovis causing otitis media that progressed to meningitis or meningoencephalitis in Japanese Black cattle. M. bovis was detected in 10 cases by a loop-mediated isothermal amplification method or by immunohistochemistry. One case of caseonecrotic granulomatous meningoencephalitis, one case of caseonecrotic granulomatous meningitis, one case of suppurative meningoencephalitis, eight cases of eustachitis, nine cases of tonsillitis and six cases of suppurative bronchopneumonia were identified by histopathological examination. M. bovis antigen was detected in the eustachian tubes of eight cases. In nine cases, M. bovis was also detected in tonsillar epithelial crypts and lumina, in intraluminal inflammatory cells and in the epithelial cells of minor salivary glands located around the eustachian tubes and tonsils. The results suggest that M. bovis can infect and colonize the tonsils and enter the eustachian tubes, causing otitis media, which, in cases of chronic infection, can progress to meningitis.
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Fujimoto Y., Inoue H., Kanda T., Ijiri M., Uemura R.
Avian diseases 65 ( 3 ) 401 - 405 2021.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Avian diseases
Colibacillosis, an infectious disease of chickens, is caused by avian pathogenic Escherichia coli (APEC); however, in addition to APEC, other pathogens are also frequently isolated from chickens affected with colibacillosis. Therefore, experimental infections in chickens are necessary to evaluate the pathogenicity of APEC isolates. Recent studies have shown that embryo lethality assays can be used as an alternative method to evaluate the pathogenicity of E. coli. In this study, to determine the important virulence genes associated with the pathogenicity of E. coli, 67 strains of E. coli that possessed different combinations of eight representative virulence genes (cva/cvi, vat, tsh, iucD, papC, irp2, iss, and astA) were isolated from broilers with colibacillosis in Japan, and the chicken embryo lethal assay was conducted. The genes vat, papC, and irp2 showed strong correlation with the level of virulence in E. coli. Our study provides useful information about the important virulence-associated genes in relation to the pathogenicity of E. coli in Japanese chickens.
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Toya R, Sasaki Y, Uemura R, Sueyoshi M
The Journal of veterinary medical science 83 ( 2 ) 322 - 328 2021.2
Language：English Publishing type：Research paper (scientific journal) Publisher：公益社団法人 日本獣医学会
In Japan, reducing the use of antimicrobials in pig production is a significant problem. However, there are no published reports concerning porcine disease treatment, as related to the age of the pigs and the indications (e.g., organ system) in Japan. In this study, we analyzed the prescription records of 17 farrow-to-finish farms from 2014 to 2018 in southern Kyushu, Japan. The farms' antimicrobial usage was calculated as the active ingredient per population correction unit (PCU) or the number of treated pigs per PCU using the defined daily dose. All data were analyzed according to the indications and production stages (sows, suckling pigs, post-weaning pigs, and fattening pigs). In terms of active ingredients/PCU, tetracyclines were the most commonly used (43.2–59.3%), and the largest amounts of antimicrobials administered through feeds were for treating the respiratory organs of fattening pigs. In terms of the number of treated pigs/PCU, tetracyclines were most frequently used (16.3–31.1%), and a high frequency of antimicrobials administered through feeds was used for the treatment of respiratory organs in post-weaning pigs. In this study, it was confirmed that tetracyclines were used frequently as a herd treatment for respiratory diseases in post-weaning and fattening pigs in southern Kyushu, Japan. The findings suggest that it is necessary to improve the treatment and prevention of respiratory diseases in post-weaning and fattening pigs in order to reduce the frequencies of antimicrobial treatments.
DOI： 10.1292/jvms.20-0436
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Radiographic diagnosis and surgical management for successful outcomes for osteochondrosis in Japanese Black calves. Reviewed
Vishwanath, NM., Kirino, Y., Uemura, R., Kim, S., Inoue, I., Hidaka, Y.
The Journal of Veterinary Medical Science. 83 ( 1 ) 151 - 157 2021.1
Language：English Publishing type：Research paper (scientific journal)