Papers - UEMURA Ryoko
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Body surface temperature of suckling piglets measured by infrared thermography and its association with body weight change. Reviewed
Sasaki, Y., Furusho, K., Ushijima, R., Tokunaga, T., Uemura, R., Sueyoshi, M.
Japan Agricultural Research Quarterly 50 ( 4 ) 361 - 368 2016.8
Language：English Publishing type：Research paper (scientific journal)
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2010年に宮崎で発生した口蹄疫の防疫措置終了後に農場内に留置された家畜排泄物のウイルス残存性調査. Reviewed
國保健浩，真瀬昌司，亀山健一郎，池口厚男，田中康男，吉田和生，山田俊治，大橋誠一，深井克彦，森岡一樹，小野里洋行，堀井 洋一郎，西脇亜也，飛佐学，佐伯雄一，井戸田幸子，上村涼子，末吉益雄，川嶌健司.
産業動物臨床獣医学雑誌 7 ( 1 ) 1 - 8 2016.8
Language：Japanese Publishing type：Research paper (scientific journal)
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An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis. Reviewed
Higa, Y., Uemura, R., Yamazaki, W., Goto, S., Goto, Y., Sueyoshi, M.
The Journal of Veterinary Medical Science 78 ( 8 ) 1343 - 1346 2016.8
Language：English Publishing type：Research paper (scientific journal)
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Yamazaki, W., Uemura, R., Sekiguchi, S., Dong, J., Watanabe, S., Kirino, Y., Mekata, H., Nonaka, N., Norimine, J., Sueyoshi, M., Goto, Y., Horii, Y., Kurogi, M., Yoshino, S., Misawa, N.
Journal of Applied Microbiology 120 ( 6 ) 1711 - 1722 2016.6
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Applied Microbiology
© 2016 The Society for Applied Microbiology. Aim: To elucidate the distribution and circulation dynamics of Campylobacter and Salmonella in Japanese chicken broiler flocks. Methods and Results: A 2-year investigation of the distribution of Campylobacter and Salmonella was conducted in 25 broiler flocks at nine farms in Japan from 2013 to 2014. Campylobacter and Salmonella tested positive in 11 (44·0%) and 24 (96·0%) broiler flocks respectively. One hundred and ninety-five Campylobacter and 184 Salmonella isolates were characterized into 12 Campylobacter (including two novel genotypes) and three Salmonella MLST genotypes. Only Salmonella isolation between caecal and environmental samples were significantly correlated. Further, one litter sample tested positive for Salmonella before new chicks were introduced. The Campylobacter strains rapidly lost culturability within 2-18 days; in contrast, the Salmonella strains survived from 64-211 days in artificially inoculated water samples. Conclusion: No persistent circulation-mediated Campylobacter contamination was observed. In contrast, circulation of Salmonella in broiler houses was seen, apparently due to the litter excreted from broiler flocks, as well as Salmonella-contaminated water and feed. Significance and Impact of the Study: This paper provides the distribution, genotypic data and circulation dynamics of Campylobacter and Salmonella as recently observed in Japanese chicken broiler farms.
DOI： 10.1111/jam.13141
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SASAKI Yosuke, SEKIGUCHI Satoshi, UEMURA Ryoko, SUEYOSHI Masuo
Journal of Veterinary Medical Science 78 ( 2 ) 333 - 335 2016
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF VETERINARY SCIENCE
This study compared the reproductive and growth performances of pigs before and after depopulation and restocking after a foot-and-mouth disease outbreak in Japan. Data for the time period before and after depopulation and restocking were obtained from three farrow-to-finish farms. As a result of depopulation and restocking, hygiene levels were improved, and common infectious diseases, such as porcine reproductive and respiratory syndrome and Aujeszky's disease, remained undetected on the farms. Compared with before depopulation, reproductive and growth performances improved after depopulation; the number of total pigs born was higher, the postweaning mortality rate was lower, and the age at slaughter was lower (<i>P</i><0.05). In summary, depopulation and restocking improved the reproductive and growth performances of pigs.
DOI： 10.1292/jvms.15-0013
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口蹄疫等の防疫用移動式レンダリング装置から排出される加熱破砕物の充填・運搬手法の検討. Reviewed
田中康男，八木行雄，末吉益雄，勝田 賢，宮崎綾子，鈴木亨，池口厚男，會田紀雄，石田三佳，中久保亮，上村涼子，橋本仁康，弓削正昭，大崎慎人，犬丸茂樹，荻野暁史。山下恭広.
家畜衛生学雑誌 40 ( 4 ) 203 - 213 2015.4
Language：Japanese Publishing type：Research paper (scientific journal)
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Sasaki Y., Uemura R., Sekiguchi S., Takahashi T., Fujii Y., Sueyoshi M.
British Poultry Science 55 ( 6 ) 737 - 744 2014.11
Language：English Publishing type：Research paper (scientific journal) Publisher：British Poultry Science
© 2014, © 2014 British Poultry Science Ltd. Abstract: 1. The present study determined descriptive values of the main production measurements of flocks and assessed the relationship between these measurements and related management factors in Japanese commercial broiler farms. 2. The data set included 5060 flock records from 183 farms. The production index was calculated as follows: liveability × average daily gain/feed conversion ratio × 10. Management factors included in the analysis were broiler breeder age, the time interval between successive flocks, the season of placement and stocking density. 3. The mean (±SD) production index was 283.9 ± 28.83. Management factors significantly associated with a decreased production index were low broiler breeder age, flocks placed in summer and high stocking density (P < 0.05). 4. In regard to an interaction for the production index, flocks with high stocking density had a lower production index than those with low stocking density in flocks with a low broiler breeder age (P < 0.05). In summer, flocks with a short time interval between successive flocks had a lower production index than those with an intermediate or long time interval (P < 0.05). 5. The present study identified factors related to flock performance. The knowledge obtained from this analysis will contribute to improve flock performance by optimising management.
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口蹄疫等の防疫における移動式レンダリング装置活用のための技術開発. Reviewed
八木行雄, 末吉益雄, 勝田賢, 宮崎綾子, 鈴木亨, 田中康男, 池口厚男, 會田紀雄, 石田三佳, 中久保亮, 上村涼子, 橋本仁康, 弓削正昭, 大崎慎人, 犬丸茂樹.
家畜衛生学雑誌 39 149 - 156 2014
Language：Japanese Publishing type：Research paper (scientific journal)
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An assessment of reproductive and lifetime performances of Kagoshima Berkshire gilts and sows. Reviewed
Sasaki, Y., Tokunaga, T., Uemura, R., and Sueyoshi, M.
Animal Science Journal 59 ( 10 ) 601 - 606 2013.10
Language：English Publishing type：Research paper (scientific journal)
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口蹄疫防疫措置の終了した発生農場に留置された家畜排泄物の状態
１１） 井戸田幸子, 飛佐 学, 上村涼子, 堀井洋一郎, 末吉益雄, 石井康之, 西脇亜也, 山本直之
日本草地学会誌 58 277 - 279 2013.1
Language：Japanese Publishing type：Research paper (scientific journal)
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The investigation of respiratory infectious mycoplasmas of cattle in Japan. Reviewed
R. Uemura, K. Nakamori, M. Sueyoshi
J. Jpn. Vet. Med. Assoc. 62 ( 11 ) 871 - 875 2012.11
Language：Japanese Publishing type：Research paper (scientific journal)
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Yamazaki W., Kumeda Y., Uemura R., Misawa N.
Food Microbiology 28 ( 6 ) 1238 - 1241 2011.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Food Microbiology
We investigated the efficacy of a loop-mediated isothermal amplification (LAMP) assay for rapid screening of seafood samples naturally contaminated with Vibrio parahaemolyticus. A total of 171 seafood samples enriched in alkaline peptone water (APW) were assessed by LAMP assay and conventional culture methods, which consist of a combination of APW enrichment culture and plating onto CHROMagar Vibrio and TCBS agars. Compared with V. parahaemolyticus isolation using the conventional culture test, LAMP results showed 100% (30/30) and 90.8% (128/141) sensitivity and specificity, respectively. The conventional culture test required more than 3 days to isolate and identify V. parahaemolyticus in the APW enrichment culture. In contrast, the LAMP assay was markedly faster, requiring less than 60 min from the beginning of DNA extraction to final detection of V. parahaemolyticus. In total, the LAMP assay required 17-19 h from the beginning of enrichment culture to final determination. This is the first report of the LAMP assay for rapid screening of seafood samples naturally contaminated by V. parahaemolyticus. © 2011 Elsevier Ltd.
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Uemura R., Sueyoshi M., Nagatomo H.
Journal of Veterinary Medical Science 72 ( 12 ) 1661 - 1663 2010.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Veterinary Medical Science
We examined the susceptibility in vitro of 99 isolates of Mycoplasma (M. bovirhinis, M. bovis, M. alkalescens and M. bovoculi) and one of Acholeplasma laidlawii collected in 2008 and 2009 from Japanese homebred cattle and cows imported from Australia to 13 antimicrobial agents. Mycoplasma species isolated from homebred cattle were susceptible to spectinomycin and fluoroquinolones but resistant to macrolides, tetracyclines, thiamphenicol and flumequine. Kanamycin, lincomycin and chloramphenicol maintained intermediate effectiveness against these species. The isolates from the imported cows maintained sufficient susceptibility to all antimicrobials examined except for erythromycin and thiamphenicol. These results showed that four species of Mycoplasma isolated from Japanese homebred cattle had obviously higher drug resistance compared with previous reports, even within this decade.
DOI： 10.1292/jvms.10-0165
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An atypical porcine proliferative enteropathy of malgrowth piglets. Reviewed
M. Sueyoshi, A. Arikawa, R. Uemura K. Nakamori and H. Nagatomo
Journal of Veterinary Medical Science 72 ( 11 ) 1483 - 1485 2010.11
Language：English Publishing type：Research paper (scientific journal)
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Secondary Mass-Outbreak of Enterotoxaemia with Escherichia coli on a Pig Farm
YUJI Hiroyuki, SUEYOSHI Masuo, NAGATOMO Hiroshi, UEMURA Ryoko, TAURA Yasuho
Journal of the Japan Veterinary Medical Association 62 ( 3 ) 211 - 214 2009.3
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Veterinary Medical Association
The acute death of postweaning piglets occurred persistently from October 1997 until June 2005 on a pig farm. The appearance of the affected piglets showed remarkable palpebral edema in the first peak, and diarrhea was confirmed as a prodromal symptom in the latter peak. The piglets were diagnosed as having enterotoxaemia with <i>Escherichia coli</i>. A total of approximately 12,000 piglets died in this case.
DOI： 10.12935/jvma.62.211
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Uemura R., Sueyoshi M., Taura Y., Nagatomo H.
Journal of Veterinary Medical Science 66 ( 8 ) 899 - 903 2004.8
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Veterinary Medical Science
Edema disease (ED) of pigs is an enterotoxaemic disease caused by enterotoxaemic Escherichia coli (ETEEC) infection. Antimicrobial therapy for pigs with ED is controversial because it may induce death of sickish piglets. In this study, we investigated the effects in vitro of 7 antimicrobial agents, ampicillin, gentamicin, colistin, bicozamycin, fosfomycin, sulfamethoxazole- trimethoprim and enrofloxacin, on the release and production of shiga toxin (Stx) 2e by ETEEC strains. We found that more Stx 2e accumulated in the bacterial cells than was released into supernatant. Associated with inhibition of cell wall synthesis, the exposure to ampicillin or fosfomycin increased the release of Stx 2e. The production levels of Stx 2e in all antimicrobial-treated cultures were equal to the level in the control or less than in the control. These results suggest that cell wall synthesis inhibitors, such as ampicillin and fosfomycin, may change for the worse in the signs in ETEEC infectious pigs. On the other hand, gentamicin, colistin, bicozamycin and enrofloxac in may be useful for the treatment of pigs with ED.
DOI： 10.1292/jvms.66.899
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Protracted Mass Occurrences of Sudden Death among Piglets with Escherichia coli Enterotoxaemia Reviewed
UEMURA Ryoko, SUEYOSHI Masuo, TAURA Yasuho, NAGATOMO Hiroshi
Journal of the Japan Veterinary Medical Association 57 ( 4 ) 231 - 234 2004.4
Language：Japanese Publishing type：Research paper (scientific journal)
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Uemura R., Sueyoshi M., Nagayoshi M., Nagatomo H.
Microbiology and Immunology 47 ( 1 ) 57 - 61 2003.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Microbiology and Immunology
Fifty-seven Shiga toxin-producing Escherichia coli (STEC) strains isolated from pigs with edema disease (ED) from 1997 to 2001 in Japan were examined for antimicrobial susceptibilities. The susceptibilities were compared with those of E. coli ATCC 23546 isolated from pig with ED in the 1950's. Consequently, the isolated STECs showed high susceptibility to peptides and bicozamycin in a way similar to the reference strain. On the other hand, the STECs showed low susceptibility to beta-lactams, tetracyclines, novobiocin, fosfomycin, trimethoprim, and old quinolones. It became clear that the susceptibilities of the isolated STECs had diminished in regard to antimicrobials.
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Nagatomo H., Takegahara Y., Sonoda T., Yamaguchi A., Uemura R., Hagiwara S., Sueyoshi M.
Veterinary Microbiology 82 ( 3 ) 223 - 232 2001.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Veterinary Microbiology
A comparison of the persistence of mycoplasmas in animals was carried out. When inoculated into liquid media, strains of Mycoplasma bovis, M. arginini, Acholeplasma laidlawii, and A. axanthum persisted for 59-185 days post-inoculation. The survival periods were not significantly influenced by temperature (4, 30, 37°C, and room temperature). The survival periods for M. bovigenitalium, M. gallisepticum, M. bovirhinis, and M. gateae ranged from < 7 to 185 days depending on medium components and temperature. Further, it was determined that strains of M. bovigenitalium, M. bovis, M. bovirhinis, M. arginini, and A. laidlawii persisted in a dry paper disc for at most 28, 126, 154, 56 and over > 168 days at 4°C, respectively. At 4°C, strains of M. gallisepticum, M. columborale, M. edwardii, M. felis, and M. gateae survived for at most 28, 21, 42, 28, 28 and 70 days, respectively. At 30°C, strains of M. bovis, M. bovirhinis, M. arginini, A. laidlawii, and M. gallisepticum persisted for at most 28, 84, 56, > 168 and 14 days, respectively, but strains of M. gallisepticum, M. columborale, M. edwardii, M. felis, M. gateae, and U. diversum did not survive for more than 14 days. In an outdoor environment, strains of M. bovirhinis and A. laidlawii survived for at most 28 and 14 days, respectively. Finally, it was found that 14 isolates of M. gallisepticum persisted for periods similar to those of the reference strains. The results under dry conditions at a variety of temperatures presented contribute to understanding the epizootiology of mycoplasmal infections in the field. © 2001 Elsevier Science B.V. All rights reserved.
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Effect of Bicozamycin on the Eradication of Shiga Toxin-Producing Escherichia coli in Calves Reviewed
Misawa Naoaki, Sueyoshi Masuo, Uemura Ryoko, KAKEMIZU Yoshihiro, KAWASHIMA Kumiko, NAGATOMO Hiroshi, KONDO Fusao, MURAKAMI Takayuki, TAKAHASHI Yoshihiro
Microbiology and immunology 44 ( 11 ) 891 - 896 2000.11
Language：English Publishing type：Research paper (scientific journal) Publisher：Center for Academic Pub. Japan