論文 - 大澤 健司
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IRSHAD Abdul Razaq, SASAKI Taihei, KUBO Tomoaki, ODASHIMA Naoyuki, KATANO Keiji, OSAWA Takeshi, TAKAHASHI Toru, IZAIKE Yoshiaki
Journal of Reproduction and Development 61 ( 5 ) 485 - 488 2015年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT
The objectives of the present study were to develop a programmable piggyback syringe pump for bovine superovulation and to evaluate the effects of a four-times-a-day injection regimen using the pump. Non-lactating Holstein cows were treated with a total of 30 armour units of porcine FSH by injection four times a day with the pump (study, n = 9) or injection twice a day manually (control, n = 9) for four consecutive days from D10 of the estrous cycle. The pump-driven program successfully induced superovulation in all cows tested. The numbers of small (3– < 5 mm in diameter) and large (≥ 10 mm in diameter) follicles were greater in the study group on D11-13 and D14, respectively. There were fewer unovulated follicles detected on D21 (7 days after estrus) in the study group than in the control group (1.2 ± 0.4 and 3.2 ± 0.6, respectively).
DOI： 10.1262/jrd.2015-047
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EL-SHEIKH ALI Hossam, KITAHARA Go, TAMURA Youji, KOBAYASHI Ikuo, HEMMI Koichiro, ZAABEL Samy, OSAWA Takeshi
日本繁殖生物学会 講演要旨集 108 ( 0 ) P - 21-P-21 2015年
記述言語：英語 掲載種別：研究論文（その他学術会議資料等） 出版者・発行元：日本繁殖生物学会
During summer, the cow is 3.7 times more likely to lose embryo in comparison to winter. The present study aimed to investigate the effect of heat stress on the thermal environment of different portions of the female genital tract in comparison to winter and spring. Three non-pregnant Japanese Black cows were investigated using a digital thermometer with special design to record the temperature of vagina (VT), cervix (CT), uterine body (UBT) and uterine horns (UHT) on D0 (D0 = heat), 1, 2, 3 and 8 of the estrous cycle (between 0630–0800 h), D0 and D8 was selected to represent the day of artificial insemination (AI) and embryo transfer (ET), respectively, under field condition. Moreover, D1, 2 and 3 were selected to represent the process of fertilization and early embryogenesis. During the experiment, temperature humidity index (THI) was recorded. This experimental design was carried out in winter, spring and summer in Miyazaki, Japan. The THI in summer was higher than the alert THI (i.e. THI > 74), indicating heat stress and was significantly higher (P ˂ 0.001) than winter and spring (78.45 ± 0.32 <i>vs</i>. 60.26 ± 1.20 and 68.51 ± 0.80, respectively). The VT, CT, UBT and UHT were significantly (P < 0.001) elevated during summer in comparison to winter and spring. Moreover, THI was positively correlated (P < 0.001) with VT, CT, UBT and UHT. Based on this correlation, we developed a mathematical model to predict the temperature of different portions of the female genital tract based on THI. In conclusion, heat stress compromises the thermal environment of different portions of the female genital tract which could passively affect gametes and/or embryo with subsequent reduction of pregnancy rate in the cow.
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Persistence of uterine bacterial infection, and associations with endometritis and ovarian function in postpartum dairy cows 査読あり
Ghanem ME, Tezuka E, Devkota B, Izaike Y, Osawa T
Journal of Reproduction and Development 61 ( 1 ) 54 - 60 2014年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1262/jrd.2014-051
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Effects of stillbirth and dystocia on subsequent reproductive performance in Japanese Black cattle
Sasaki Y., Uematsu M., Kitahara G., Osawa T., Osawa T., Sueyoshi M., Sueyoshi M.
Veterinary Journal 200 ( 3 ) 462 - 463 2014年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Journal
The objective of this study was to determine the effects of stillbirth and dystocia on subsequent reproductive performance in Japanese Black cattle. Data were collected for 34,763 calvings from 13,186 animals on 826 farms. Stillbirth was associated with a decreased first service conception rate compared to cows with a normal calving (44.4 versus 54.0%, respectively, P < 0.001). Cows with a normal calving required fewer artificial inseminations per conception and had a lower stillbirth rate at subsequent parity than those with stillbirth and dystocia (P < 0.001). Japanese Black cows with stillbirth and dystocia had inferior subsequent reproductive performance compared with those with a normal calving. © 2014 Elsevier Ltd.
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Effect of postbreeding intramuscular administration of deslorelin acetate on plasma LH and progesterone concentrations and conception rate in mares 査読あり
Kino Satoshi, Osawa Takeshi, Mizuno Shuji, Watanabe Gen, Taya Kazuyoshi, Izaike Yoshiaki
Journal of Equine Science 25 ( 1 ) 23 - 27 2014年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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International perspectives on the impacts of reproductive technologies on food production in Asia 招待あり
Takeshi Osawa
Advances in Experimental Medicine and Biology 752 213 - 228 2014年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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EL-SHEIKH ALI Hossam, KITAHARA Go, NIBE Kazumi, YAMAGUCHI Ryoji, OSAWA Takeshi
日本繁殖生物学会 講演要旨集 107 ( 0 ) P - 26-P-26 2014年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本繁殖生物学会
The current study aimed to evaluate the diagnostic value of plasma anti-Müllerian hormone (AMH) as a biomarker for sex cord-stromal tumors (SCST) in cattle in comparison to immunoreactive inhibin (ir-INH), estradiol-17β (E<sub>2</sub>) and testosterone (T). Therefore, we conducted two experiments. In experiment 1, four clinically healthy Japanese Black cows had blood samples taken daily over one estrous cycle to characterize their AMH profiles throughout the normal estrous cycle. Additionally, single blood samples were collected from 21 cyclic cows to clarify the physiological range of AMH. In experiment 2, animals with histologically confirmed SCST (SCST group, n = 10) and cows affected with cystic ovarian disease (COD group, n = 8) had one blood sample taken before extraction of SCST or treatment of COD. Blood samples (n = 105) from cyclic cows (n = 25) in experiment 1 were assigned as a physiologically cyclic group (PC group). Plasma AMH, ir-INH, E<sub>2</sub> and T concentrations were assayed in all samples. Plasma AMH concentrations did not show substantial fluctuation throughout the estrous cycle. Only plasma AMH, ir-INH and E<sub>2</sub> were significantly elevated in SCST group in comparison to PC group. The area under the receiver operating characteristic curve (AUC<sub>ROC</sub>) of AMH for diagnosis of SCST was 0.99 and was significantly (P < 0.01) higher than that of ir-INH and E<sub>2</sub>. Plasma AMH at a cutoff point of ≥ 0.36 ng/mL had the highest diagnostic accuracy (99.2%), sensitivity (100%), and specificity (99.1%) compared with ir-INH and E<sub>2</sub>. In conclusion, unilateral ovarian enlargement accompanied with plasma AMH concentrations ≥ 0.36 ng/mL is considered diagnostic for bovine SCST.
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Effect of Postbreeding Intramuscular Administration of Deslorelin Acetate on Plasma LH and Progesterone Concentrations and Conception Rate in Mares.
Kino S, Osawa T, Mizuno S, Watanabe G, Taya K, Izaike Y
Journal of equine science 25 ( 1 ) 23 - 7 2014年
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Plasma anti-Mullerian hormone as a biomarker for bovine granulosa-theca cell tumors: comparison with immunoreactive inhibin and ovarian steroid concentrations 査読あり
El-Sheikh Ali Hossam, Kitahara Go, Nibe Kazumi, Yamaguchi Ryoji, Horii Yoichiro, Zaabel Samy, Osawa Takeshi
Theriogenology 90 940 - 949 2013年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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El-Sheikh Ali H., Kitahara G., Nibe K., Yamaguchi R., Horii Y., Zaabel S., Osawa T.
Theriogenology 80 ( 8 ) 940 - 949 2013年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Theriogenology
Granulosa-theca cell tumors (GTCTs) are the most frequently reported ovarian tumors in cattle. Clinically, GTCTs could be confused with other ovarian abnormalities; therefore, the only definitive diagnosis for such tumors is histopathology of a biopsy from the affected ovary. However, this is an invasive technique and unsuitable for farm conditions. As a result, the key aim of this study was to evaluate the diagnostic value of anti-Müllerian hormone (AMH), a glycoprotein hormone that is synthesized exclusively by ovarian granulosa cells, as a sensitive noninvasive biomarker for diagnosing GTCTs in cattle. To achieve this aim, we conducted two experiments. In experiment 1, four clinically healthy Japanese Black cows had blood samples taken daily over one estrous cycle to characterize their AMH profiles throughout the estrous cycle. Additionally, single blood samples were collected from 21 cyclic cows to clarify the physiological range of AMH. In experiment 2, cows with histologically confirmed GTCT (GTCT group, n = 9) and cows affected with cystic ovarian disease (COD group, n = 8) had one blood sample taken before extraction of the tumorous ovary or therapeutic treatment for the COD. Blood samples (n = 105) from cyclic cows (n = 25) in experiment 1 were assigned as a physiologically cyclic group (PC group). Plasma AMH, immunoreactive inhibin (ir-INH), estradiol-17β (E 2 ), testosterone (T), and progesterone (P 4 ) concentrations were assayed in all samples. In experiment 1, the mean plasma AMH concentration was 0.09 ± 0.003 ng/mL and did not show substantial fluctuation throughout the estrous cycle. In experiment 2, plasma AMH, ir-INH, and E 2 concentrations were significantly elevated in the GTCT group in comparison with the PC group; among these parameters, only the AMH concentrations were significantly higher in the GTCT group than in the COD group. The area under the receiver operating characteristic curve of AMH for diagnosis of GTCT was 0.99 and was significantly higher than that of ir-INH (P < 0.001) and E 2 (P < 0.01). Moreover, the AMH at a cutoff point of ≥0.36 ng/mL had the highest diagnostic accuracy (99.2%), sensitivity (100%), and specificity (99.1%) compared with the other tested parameters. In conclusion, plasma AMH concentration is probably a more reliable and sensitive biomarker for bovine GTCTs than the concentrations of ir-INH or ovarian steroids. © 2013 Elsevier Inc.
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Risk factors for stillbirth and dystocia in Japanese Black cattle 査読あり
Uematsu Mizuho, Sasaki Yosuke, Kitahara, Go, Sameshima Hiroshi, Osawa Takeshi
The Veterinary Journal 198 212 - 216 2013年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Prevalence of antibodies against Parainfluenza virus type 3, Respiratory syncitial virus and bovine Herpesvirus type 1 in sheep from Northern Prefectures of Japan 査読あり
Massimo Giangaspero, Giovanni Savini, Riccardo Orusa, Takeshi Osawa, Ryô Harasawa
Veterinaria Italiana 49 ( 3 ) 285 - 289 2013年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Fluctuation in plasma bone metabolic markers in multiparous and primiparous Holstein cows during early to peak lactation 査読あり
Devkota, B., Takahashi, M., Sasaki, K., Osawa, T., Izaike, Y., Yamagishi, N.
Journal of Veterinary Medical Science 2013年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Giangaspero, M., Orusa, R., Savini, G., Di Gennaro, A., Takeshi Osawa, T., Harasawa, R.
Clinical Microbiology 2013年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.4172/cmo.1000104
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Serological survey to determine the occurrence of malignant catarrhal fever infection in the Japanese small ruminant population from northern districts 査読あり
Giangaspero, M., Savini, G., Osawa, T., Harasawa, R.
Journal of Veterinary Medical Science 2013年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Epidemiological survey for Toxoplasma gondii, Chlamydia psittaci var. ovis, Mycobacterium paratuberculosis, Coxiella burnetii, Brucella spp., Leptospirosis, and orf virus among sheep from northern districts of Japan 査読あり
Giangaspero, M., Bonfini, B., Orusa, R., Osawa, T., Harasawa, R.
Journal of Veterinary Medical Science 75 ( 5 ) 679 - 684 2013年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Epidemiological survey for Toxoplasma gondii, Chlamydia psittaci var. ovis, Mycobacterium paratuberculosis, Coxiella burnetii, Brucella spp., leptospirosis and Orf virus among sheep from northern districts of Japan.
Giangaspero M, Bonfini B, Orusa R, Savini G, Osawa T, Harasawa R
The Journal of veterinary medical science 75 ( 5 ) 679 - 84 2013年
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Fluctuation in plasma bone metabolic markers in multiparous and primiparous Holstein cows during early to peak lactation.
Devkota B, Takahashi M, Sasaki K, Osawa T, Izaike Y, Yamagishi N
The Journal of veterinary medical science 75 ( 9 ) 1257 - 60 2013年
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Serological survey to determine the occurrence of malignant catarrhal fever infection in the Japanese small ruminant population from northern districts. 査読あり
Giangaspero M, Savini G, Osawa T, Harasawa R
The Journal of veterinary medical science 75 ( 6 ) 815 - 8 2013年
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Experimental infection of pregnant cows with noncytopathogenic bovine viral diarrhea virus between days 26 and 50 postbreeding 査読あり
57 Tsuboi, T., Osawa, T., Hirata, T-I., Kawashima, K., Kimura, K., Haritani, M.
Research in Veterinary Science 94 ( 3 ) 803 - 805 2012年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）