論文 - 目堅 博久
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Comparison of two agar gel immunodiffusion protocols for diagnosing equine infectious anemia. 査読あり 国際誌
Nemoto M, Yamanaka T, Bannai H, Tsujimura K, Ueno T, Mekata H, Yoshida A, Koyama A, Kokado H
Journal of Veterinary Medical Science 80 ( 8 ) 1245 - 1247 2018年6月
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Molecular epidemiological survey and phylogenetic analysis of bovine influenza D virus in Japan. 査読あり 国際誌
Mekata, H., Yamamoto, M., Hamabe, S., Tanaka, H., Omatsu, T., Mizutani, T., Hause, B.M., Okabayashi, T.
Transboundary and emerging diseases 65 ( 2 ) e355 - e360 2018年4月
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New hematological key for bovine leukemia virus-infected Japanese Black cattle. 査読あり 国際誌
Mekata H, Yamamoto M, Kirino Y, Sekiguchi S, Konnai S, Horii Y, Norimine J
Journal of Veterinary Medical Science 80 ( 2 ) 316 - 319 2018年2月
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Dembo-PCR technique for the detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs. 査読あり 国際誌
Rahpaya, SS, Tsuchiaka S, Kishimoto M, Oba M, Katayama Y, Nunomura Y, Kokawa S, Kimura T, Kobayashi A, Kirino Y, Okabayashi T, Nonaka N, Mekata H, Aoki H, Shiokawa M, Umetsu M, Morita T, Hasebe A, Otsu K, Asai T, Yamaguchi T, Makino S, Murata Y, Abi AJ, Omatsu T, Mizutani T
Journal of veterinary science 2017年12月
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Detection and molecular characterization of equine infectious anemia virus in Mongolian horses. 査読あり 国際誌
Sharav, T., Konnai, S., Ochirkhuu, N., Ts, E.O., Mekata, H., Sakoda, Y., Umemura, T., Murata, S., Chultemdorj, T., Ohashi, K.
Journal of Veterinary Medical Science 79 ( 11 ) 1884 - 1888 2017年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
The genetic characterization and actual prevalence of EIAV in Mongolian horse in the disease endemic region is currently unknown. Here, 11 of 776 horse serum samples from four Mongolian provinces tested positive on agar gel immunodiffusion test. Genomic DNA extracted from all seropositive samples was subjected to nested PCR assay. Among these, three samples tested positive with nested PCR assay and were identified by sequencing analysis based on long termination repeat and tat gene of the virus. Two of the three sequences were identical, with 94.0% identity with the third. These two independent Mongolian EIAV sequences were retained functional motifs, with no dramatic changes but some variability in the U5 region; they were clustered with genotypes from European countries but not with those from China, U.S.A., or Japan.
DOI： 10.1292/jvms.17-0202
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Intrauterine infection with bovine leukemia virus in pregnant dam with high viral load. 査読あり 国際誌
Sajiki, Y., Konnai, S., Nishimori, A., Okagawa, T., Maekawa, N., Goto, S., Nagano, M., Kohara, J., Kitano, N., Takahashi, T., Tajima, M., Mekata, H., Horn, Y., Murata, S., Ohashi, K.
Journal of Veterinary Medical Science 79 ( 12 ) 2036 - 2039 2017年11月
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Hayashi, T., Mekata, H., Sekiguchi, S., Kirino, Y., Mitoma, S., Honkawa, K., Horii, Y., Norimine, J.
Journal of Veterinary Medical Science 12 ( 79 ) 1552 - 1555 2017年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本獣医学会
The bovine MHC (BoLA) class II <i>DRB3</i> alleles are associated with polyclonal expansion of lymphocytes caused by bovine leukemia virus (BLV) infection in cattle. To examine whether the <i>DRB3*0902</i> allele, one of the resistance-associated alleles, is associated with the proviral load, we measured BLV proviral load of BLV-infected cattle and clarified their <i>DRB3</i> alleles. Fifty-seven animals with <i>DRB3*0902</i> were identified out of 835 BLV-infected cattle and had significantly lower proviral load (<i>P</i><0.000001) compared with the rest of the infected animals, in both Japanese Black and Holstein cattle. This result strongly indicates that the BoLA class II DRA/DRB3*0902 molecule plays an important immunological role in suppressing viral replication, resulting in resistance to the disease progression.
DOI： 10.1292/jvms.16-0601
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Phylogenetic analysis of env gene of bovine leukemia virus strains spread in Miyazaki prefecture, Japan 査読あり 国際誌
Marawan, M.A., Mekata, H., Hayashi, T., Sekiguchi, S., Kirino, Y., Horii, Y., Moustafa, A.M., Arnaout, F.K., Galila, E.S.M., Norimine, J.
Journal of Veterinary Medical Science 79 ( 5 ) 912 - 916 2017年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1292/jvms.17-0055
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Sabine, I., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Farid, A., Goto, Y., Horii, Y., Yamazaki, W.
Food Control 74 107 - 111 2017年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Food Control
© 2016 Elsevier Ltd In a two-year survey of the 24 Japanese broiler chicken flocks at 9 farms from 2013 to 2014, C. jejuni/C. coli prevalence was assessed in a total of 131 slaughtered broiler chicken cecal samples by conventional culture methods and loop-mediated isothermal amplification (LAMP) assay. While 93 samples were C. jejuni/C. coli-negative, 38 (29.0%) showed Campylobacter loads of between 6.4 and 9.0 log CFU/g of ceca in conventional culture methods. The performance of LAMP assay was 100% accurate in terms of diagnostic sensitivity (38/38), specificity (93/93). Furthermore, LAMP assay enabled direct screening of C. jejuni and C. coli in cecal samples from broiler chicken chickens as rapid and cost-effective detection within 90 min and less than 1 US dollar, which can help monitor release of Campylobacter-contaminated chicken into the food chain, thereby reducing the incidence and public health risk of campylobacteriosis. Seasonal changes in C. jejuni and C. coli prevalence in broiler chicken ceca were significantly correlated with the frequency of food poisoning incidents caused by these bacteria in Japan.
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Sabike, I., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Okabayashi, T., Goto, Y., Yamazaki, W.
Frontiers in Microbiology 7 ( SEP ) 1582 2016年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Microbiology
© 2016 Sabike, Uemura, Kirino, Mekata, Sekiguchi, Okabayashi, Goto and Yamazaki. Rapid identification of Campylobacter-positive flocks before slaughter, following freezing and heat treatment for the Campylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. We evaluated a loop-mediated isothermal amplification (LAMP) assay for the direct screening of naturally contaminated chicken cloacal swabs for C. jejuni/C. coli to compare this assay with conventional qu antitative culture methods. In a comparison study of 165 broilers, the LAMP assay showed 82.8% (48/58 by conventional culture) sensitivity, 100% (107/107) specificity, 100% (48/48) positive predictive value (PPV), and 91.5% (107/117) negative predictive value (NPV). In a comparison of 55 flocks, LAMP showed 90.5% (19/21) sensitivity, 100% (34/34) specificity, 100% (19/19) PPV, and 94.4% (34/36) NPV. In the cumulative total of 28 farm-level comparisons, LAMP showed 100% (12/12) sensitivity, 100% (16/16) specificity, 100% (12/12) PPV, and 100% (16/16) NPV. The LAMP assay required less than 90 min from the arrival of the fecal samples to final results in the laboratory. This suggests that the LAMP assay will facilitate the identification of C. jejuni/C. coli-positive broiler flocks at the farm level or in slaughterhouses before slaughtering, which would make it an effective tool in preventing the spread of Campylobacter contamination.
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Nationwide distribution of bovine influenza D virus infection in Japan 査読あり 国際誌
Horimoto, T., Hiono, T., Mekata, H., Odagiri, T., Lei, Z., Kobayashi, T., Norimine, J., Inoshima, Y., Hikono, H., Murakami, K., Sato, R., Murakami, H., Sakaguchi, M., Ishii, K., Ando, T., Otomaru, K., Ozawa, M., Sakoda, Y., Murakami, S.
PLoS ONE 11 ( 9 ) e0163828 2016年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：PLoS ONE
© 2016 Horimoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Cattle are major reservoirs of the provisionally named influenza D virus, which is potentially involved in the bovine respiratory disease complex. Here, we conducted a serological survey for the influenza D virus in Japan, using archived bovine serum samples collected during 2010-2016 from several herds of apparently healthy cattle in various regions of the country. We found sero-positive cattle across all years and in all the prefectural regions tested, with a total positivity rate of 30.5%, although the positivity rates varied among regions (13.5-50.0%). There was no significant difference in positivity rates for Holstein and Japanese Black cattle. Positivity rates tended to increase with cattle age. The herds were clearly divided into two groups: those with a high positive rate and those with a low (or no) positive rate, indicating that horizontal transmission of the virus occurs readily within a herd. These data demonstrate that bovine influenza D viruses have been in circulation for at least 5 years countrywide, emphasizing its ubiquitous distribution in the cattle population of Japan.
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Yamazaki, W., Uemura, R., Sekiguchi, S., Dong, J., Watanabe, S., Kirino, Y., Mekata, H., Nonaka, N., Norimine, J., Sueyoshi, M., Goto, Y., Horii, Y., Kurogi, M., Yoshino, S., Misawa, N.
Journal of Applied Microbiology 120 ( 6 ) 1711 - 1722 2016年6月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Applied Microbiology
© 2016 The Society for Applied Microbiology. Aim: To elucidate the distribution and circulation dynamics of Campylobacter and Salmonella in Japanese chicken broiler flocks. Methods and Results: A 2-year investigation of the distribution of Campylobacter and Salmonella was conducted in 25 broiler flocks at nine farms in Japan from 2013 to 2014. Campylobacter and Salmonella tested positive in 11 (44·0%) and 24 (96·0%) broiler flocks respectively. One hundred and ninety-five Campylobacter and 184 Salmonella isolates were characterized into 12 Campylobacter (including two novel genotypes) and three Salmonella MLST genotypes. Only Salmonella isolation between caecal and environmental samples were significantly correlated. Further, one litter sample tested positive for Salmonella before new chicks were introduced. The Campylobacter strains rapidly lost culturability within 2-18 days; in contrast, the Salmonella strains survived from 64-211 days in artificially inoculated water samples. Conclusion: No persistent circulation-mediated Campylobacter contamination was observed. In contrast, circulation of Salmonella in broiler houses was seen, apparently due to the litter excreted from broiler flocks, as well as Salmonella-contaminated water and feed. Significance and Impact of the Study: This paper provides the distribution, genotypic data and circulation dynamics of Campylobacter and Salmonella as recently observed in Japanese chicken broiler farms.
DOI： 10.1111/jam.13141
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Six novel O genotypes from Shiga toxin-producing Escherichia coli 査読あり 国際誌
Iguchi, A., Iyoda, S., Seto, K., Nishii, H., Ohnishi, M., Mekata, H., Ogura, Y., Hayashi, T.
Frontiers in Microbiology 7 765 2016年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Microbiology
© 2016 Iguchi, Iyoda, Seto, Nishii, Ohnishi, Mekata, Ogura and Hayashi. Serotyping is one of the typing techniques used to classify strains within the same species. O-serogroup diversification shows a strong association with the genetic diversity of O-antigen biosynthesis genes. In a previous study, based on the O-antigen biosynthesis gene cluster (O-AGC) sequences of 184 known Escherichia coli O serogroups (from O1 to O187), we developed a comprehensive and practical molecular O serogrouping (O genotyping) platform using a polymerase chain reaction (PCR) method, named E. coli O-genotyping PCR. Although, the validation assay using the PCR system showed that most of the tested strains were successfully classified into one of the O genotypes, it was impossible to classify 6.1% (35/575) of the strains, suggesting the presence of novel O genotypes. In this study, we conducted sequence analysis of O-AGCs from O-genotype untypeable Shiga toxin-producing E. coli (STEC) strains and identified six novel O genotypes; OgN1, OgN8, OgN9, OgN10, OgN12 and OgN31, with unique wzx and/or wzy O-antigen processing gene sequences. Additionally, to identify these novel O-genotypes, we designed specific PCR primers. A screen of O genotypes using O-genotype untypeable strains showed 13 STEC strains were classified into five novel O genotypes. The O genotyping at the molecular level of the O-AGC would aid in the characterization of E. coli isolates and will assist future studies in STEC epidemiology and phylogeny.
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Horizontal transmission and phylogenetic analysis of bovine leukemia virus in two districts of Miyazaki, Japan. 査読あり 国際誌
Mekata H, Sekiguchi S, Konnai S, Kirino Y, Horii Y, Norimine J.
Journal of Veterinary Medical Science 77 ( 9 ) 1115 - 1120 2015年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1292/jvms.14-0624
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Expression of regulatory dendritic cell-related cytokines in cattle experimentally infected with Trypanosoma evansi. 査読あり 国際誌
Mekata H, Murata S, Mingala CN, Ohashi K, Konnai S.
Journal of Veterinary Medical Science 77 ( 8 ) 1017 - 1019 2015年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1292/jvms.15-0066
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Evaluation of the natural perinatal transmission of bovine leukaemia virus 査読あり 国際誌
Mekata H, Sekiguchi S, Konnai S, Kirino Y, Honkawa K, Nonaka N, Horii Y, Norimine J.
Veterinary Record 176 ( 10 ) 254 2015年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1136/vr.102464
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Identification of O Serotypes, Genotypes, and Virulotypes of Shiga Toxin–Producing Escherichia coli Isolates, Including Non-O157 from Beef Cattle in Japan. 査読あり 国際誌
Mekata H, Iguchi A, Kawano K, Kirino Y, Kobayashi I, Misawa N.
Journal of Food Protection 77 ( 8 ) 1269 - 1274 2014年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Imamura S., Mekata H., Kirino Y., Nakamizo M., Hirano F., Kawanishi M., Yamamoto T., Nagai H., Kijima M., Kobayashi I.
International Journal of Applied Research in Veterinary Medicine 11 ( 3 ) 189 - 193 2013年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：International Journal of Applied Research in Veterinary Medicine
To analyze the factors associated with an appearance of adverse events in calves following Histophilus somni vaccination, we investigated changes in rectal temperature, tumor necrosis factor-alpha (TNF-α) levels, and clinical signs in vaccinated Japanese Black calves (n=19). The average rectal temperature 24 hr post-vaccination was significantly higher in male calves compared with pre-vaccination. In contrast, the rectal temperature of female calves remained constant during the observation period. Febrile male calves aged over 9 months had inappetence, while the appetite of calves aged less than 8 months was normal. These results suggest that sex and age are associated with adverse effects elicited by H. somni vaccination. The amount of TNF-α in the peripheral blood derived from male calves was significantly lower than that derived from steer and female calves. Sex and/or age-related effects were not identified in reported cases of adverse events. Acute shock was the most common presentation in reported cases, typically when the vaccine was administered with another multivalent vaccine. Therefore, H. somni vaccination is considered safe when administered according to the manufacturer's instructions.
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Bovine whole-blood culture as a tool for the measurement of endotoxin activities in Gram-negative bacterial vaccines. 査読あり 国際誌
Imamura S, Nakamizo M, Kawanishi M, Nakajima N, Yamamoto K, Uchiyama M, Hirano F, Nagai H, Kijima M, Ikebuchi R, Mekata H, Murata S, Konnai S, Ohashi K
Veterinary immunology and immunopathology 153 ( 1-2 ) 153 - 8 2013年5月
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Isolation, cloning, and pathologic analysis of Trypanosoma evansi field isolates. 査読あり 国際誌
Mekata H, Konnai S, Mingala CN, Abes NS, Gutierrez CA, Dargantes AP, Witola WH, Inoue N, Onuma M, Murata S, Ohashi K
Parasitology Research 112 ( 4 ) 1513 - 1521 2013年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）