論文 - 目堅 博久
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Transcriptional profiling of inflammatory cytokine genes in African buffaloes (Syncerus caffer) infected with Theileria parva. 査読あり 国際誌
Okagawa T, Konnai S, Mekata H, Githaka N, Suzuki S, Kariuki E, Gakuya F, Kanduma E, Shirai T, Ikebuchi R, Ikenaka Y, Ishizuka M, Murata S, Ohashi K
Veterinary immunology and immunopathology 148 ( 3-4 ) 373 - 9 2012年8月
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Kinetics of regulatory dendritic cells in inflammatory responses during Trypanosoma evansi infection. 査読あり 国際誌
Mekata H, Konnai S, Mingala CN, Abes NS, Gutierrez CA, Dargantes AP, Witola WH, Inoue N, Onuma M, Murata S, Ohashi K
Parasite immunology 34 318 - 329 2012年6月
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Molecular detection of trypanosomes in cattle in South America and genetic diversity of Trypanosoma evansi based on expression-site-associated gene 6. 査読あり 国際誌
Mekata H, Konnai S, Witola WH, Inoue N, Onuma M, Ohashi K
Infection, genetics and evolution 9 ( 6 ) 1301 - 5 2009年12月
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Development and application of a quantitative real-time PCR for the diagnosis of Surra in water buffaloes. 査読あり 国際誌
Konnai S, Mekata H, Mingala CN, Abes NS, Gutierrez CA, Herrera JR, Dargantes AP, Witola WH, Cruz LC, Inoue N, Onuma M, Ohashi K
Infection, genetics and evolution 9 ( 4 ) 449 - 52 2009年7月
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Prevalence and source of trypanosome infections in field-captured vector flies (Glossina pallidipes) in southeastern Zambia. 査読あり 国際誌
Mekata H, Konnai S, Simuunza M, Chembensofu M, Kano R, Witola WH, Tembo ME, Chitambo H, Inoue N, Onuma M, Ohashi K
Journal of Veterinary Medical Science 70 ( 9 ) 923 - 8 2008年9月
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Konnai, S., Mekata, H., Odbileg, R., Simuunza, M., Chembensof, M., Witola, W.H., Tembo, M.E., Chitambo, H., Inoue, N., Onuma, M., Ohashi, K.
Vector borne and zoonotic diseases 8 ( 4 ) 565 - 73 2008年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：4
The prevalence of trypanosome infections in tsetse flies in the Chiawa area of Lower Zambezi in Zambia, with endemic trypanosomosis, was determined by a polymerase chain reaction (PCR) method that allowed the detection of trypanosome DNA and determination of the type of animal host fed on by the tsetse fly Glossina pallidipes, using tsetse-derived DNA extracts as templates. Ninety G. pallidipes (82 females and 8 males; 18.3%) of the 492 flies captured by baited biconical traps tested positive for the presence of Trypanosoma brucei species genomic DNA. Of the 90 T. brucei-positive flies, 47 (52.2%) also tested positive for vertebrate mitochondrial DNA. Sequence analysis of the vertebrate mitochondrial DNA amplicons established that they originated from 8 different vertebrate species, namely, human (Homo sapiens), African elephant (Loxodonta cyclotis), African buffalo (Syncerus caffer), waterbuck (Kobus ellipsiprymnus), roan antelope (Hippotragus equinus), greater kudu (Tragelaphus strepsiceros), warthog (Phacochoerus africanus), and goat (Capra hircus). Furthermore, to investigate the prevalence of trypanosome infections in domestic goats in the same area where trypanosomes had been detected in tsetse files, a total of 86 goats were randomly selected from 6 different herds. Among the selected goats, 36 (41.9%) were found to be positive for T. brucei species. This combined detection method would be an ideal approach not only for mass screening for infection prevalence in tsetse populations, but also for the prediction of natural reservoirs in areas endemic for trypanosomosis.