論文 - 山﨑 正夫
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食品中のナノ粒子に関する機能性研究 査読あり
山﨑正夫、山﨑有美、大島達也
化学と生物 61 ( 2 ) 78 - 84 2023年2月
担当区分：筆頭著者, 責任著者 記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Nanovesicles derived from edible plants: a new player that contributes to the function of foods
Yamasaki M., Yamasaki Y., Oshima T.
Bioscience, Biotechnology and Biochemistry 89 ( 2 ) 179 - 186 2025年2月
担当区分：筆頭著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Nano-sized vesicles are ubiquitous in vegetables, fruits, and other edible plants. We have successfully prepared nanovesicles (NVs) from over 150 edible plants. These results suggest that the daily intake of NVs from various foods and their roles in food function are promising novel approaches for explaining the health-promoting properties of edible plants. These vesicles contain RNAs, including miRNAs, similar to extracellular NVs, which play pivotal roles in cell-cell communication. Intriguingly, NVs also contain phytochemicals such as polyphenols and carotenoids that are specific to each edible plant. In conclusion, these dietary NVs have the potential to serve as functional packages to deliver RNAs or phytochemicals to target cells across species from plants to humans.
DOI： 10.1093/bbb/zbae198
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Nei S., Matsusaki T., Kawakubo H., Ogawa K., Nishiyama K., Tsend-Ayush C., Nakano T., Takeshita M., Shinyama T., Yamasaki M.
Nutrients 16 ( 17 ) 2024年9月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nutrients
ATP-binding cassette transporter subfamily G member 2 (ABCG2) is responsible for the excretion of foreign substances, such as uric acid (UA) and indoxyl sulfate (IS), from the body. Given the importance of increased ABCG2 expression in UA excretion, we investigated the enhancement of intestinal ABCG2 expression using Lactiplantibacillus plantarum 06CC2 (LP06CC2). Mice were reared on a potassium oxonate-induced high-purine model at doses of 0.02% or 0.1% LP06CC2 for three weeks. Results showed that LP06CC2 feeding resulted in increased ABCG2 expression in the small intestine. The expression level of large intestinal ABCG2 also showed a tendency to increase, suggesting upregulation of the intestinal excretion transporter ABCG2 by LP06CC2. Overall, LP06CC2 treatment increased fecal UA excretion and showed a trend towards increased fecal excretion of IS, suggesting that LP06CC2 treatment enhanced the expression of intestinal ABCG2, thereby promoting the excretion of UA and other substances from the intestinal tract.
DOI： 10.3390/nu16173042
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Takeguchi T., Kai H., Takeshita M., Miura M., Ogawa K., Nishiyama K., Yamasaki M.
Journal of Oleo Science 73 ( 7 ) 991 - 999 2024年7月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oleo Science
In this study, we evaluated the cancer cell killing activity of koji mold-derived extracts using several solvents. The koji mold lipid extract (KML) exhibited potent cytotoxicity against a human leukemia cell line. Fractionation of the KML via silica gel chromatography revealed the presence of active components in fraction (Fr.) 6. Cytotoxic effects of Fr. 6 were inhibited by the ferroptosis inhibitors, ferrostatin-1 and SRS11-92, and the iron chelator, deferoxamine. Interestingly, ferroptosis inhibitors failed to prevent the KML-induced cell death. Fr. 6 decreased the expression of glutathione peroxidase 4 (GPx4) and increased the level of peroxidized plasma membrane lipids. Furthermore, Fr. 6 decreased the intracellular glutathione levels. Overall, our results suggest that Fr. 6 included in KML induces ferroptosis in HL-60 cells.
DOI： 10.5650/jos.ess24043
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山﨑 有美, 河野 愛未, 松本 朋子, 大島 達也, 山﨑 正夫
日本食品科学工学会誌 71 ( 5 ) 181 - 181 2024年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本食品科学工学会
第71巻3号P.83–91 単位修正p.84右側51行目 （誤）100 mg/mL →（正）100 μg/mLp.85左側16行目 （誤）0.45 mm →（正）0.45 μmp.85左側30行目 （誤）1 mL →（正）1 μLp.85右側10行目 （誤）100 mg/mL →（正）100 μg/mLp.85右側20行目 （誤）2.1 mL →（正）2.1 μLp.85右側25行目 （誤）300 mL →（正）300 μLp.85右側35行目 （誤）0.4 mm →（正）0.4 μmp.86左側12行目 （誤）750 mL →（正）750 μLp.86左側30行目 （誤）188.5 mg/100 g →（正）188.5 μg/100 gp.86左側31行目 （誤）248.2 mg/100 g →（正）248.2 μg/100 gp.86右側7行目 （誤）123.7 mg →（正）123.7 μgp.86右側9行目 （誤）112.2 mg →（正）112.2 μgp.87左側1行目 （誤）149.4 mg →（正）149.4 μgp.87左側3行目 （誤）138.0 mg →（正）138.0 μgp.87左側5行目 （誤）111.7 mg →（正）111.7 μgp.88上 図6・y軸単位 （誤）(μg) →（正）(ng)p.88左側29行目 （誤）1.02 mg →（正）1.02 ngp.88右側2行目 （誤）15.62 mg →（正）15.62 ngp.88右側6行目 （誤）13.38 mg →（正）13.38 ng
DOI： 10.3136/nskkk.71.181
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ヒト腸管上皮細胞モデル (Caco-2) におけるキンカン由来β-クリプトキサンチンの膜透過性に及ぼす麹菌発酵乳飲料の影響 査読あり
山﨑 有美, 河野 愛未, 松本 朋子, 大島 達也, 山﨑 正夫
日本食品科学工学会誌 71 ( 3 ) 83 - 91 2024年3月
担当区分：最終著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本食品科学工学会
キンカンを加工し機能性成分であるβ-クリプトキサンチンの含有量を比較したところ, ペーストにより多くのβ-クリプトキサンチンが含まれ, ペーストと麹菌発酵乳飲料を混合しても, β-クリプトキサンチン含有量は変化しなかった. また, キンカンペースト添加麹菌発酵乳飲料を65 °C 及び 85 °Cで殺菌処理したところ, 65 °C試験区において多くのβ-クリプトキサンチンが残存することが示された. キンカン由来β-クリプトキサンチン－麹菌発酵乳飲料混合物のβ-クリプトキサンチン腸管吸収能を評価した結果, β-クリプトキサンチン単独区と比較し, 麹菌発酵乳飲料混合区の腸管吸収量は約14倍に上昇することが明らかとなった.
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<i>Lactiplantibacillus plantarum</i> 06CC2 upregulates intestinal ZO-1 protein and bile acid metabolism in Balb/c mice fed high-fat diet 査読あり
YAMASAKI Masao, MIYAMOTO Yuko, OGAWA Kenjirou, NISHIYAMA Kazuo, TSEND-AYUSH Chuluunbat, LI Yiran, MATSUSAKI Tatsuya, NAKANO Tomoki, TAKESHITA Masahiko, ARIMA Yuo
Bioscience of Microbiota, Food and Health advpub ( 0 ) 2024年1月
担当区分：最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：BMFH出版会
The effects of <i>Lactiplantibacillus plantarum </i>06CC2 (LP06CC2), which was isolated from a Mongolian dairy product, on lipid metabolism and intestinal tight junction–related proteins in Balb/c mice fed a high-fat diet (HFD) were evaluated. The mice were fed the HFD for eight weeks, and the plasma and hepatic lipid parameters, as well as the intestinal tight junction–related factors, were evaluated. LP06CC2 slightly reduced the adipose tissue mass. Further, it dose-dependently decreased plasma total cholesterol (TC). The HFD tended to increase the plasma level of endotoxin and suppressed intestinal ZO-1 expression, whereas a low LP06CC2 dose increased ZO-1 expression and tended to reduce the plasma lipopolysaccharide level. Furthermore, a low LP06CC2 dose facilitated a moderate accumulation of Lactobacillales, a significant decrease in <i>Clostridium </i>cluste<i>r </i>IV, and an increase in <i>Clostridium </i>cluster<i></i>XVIII. The results obtained from analyzing the bile acids (BAs) in feces and cecum contents exhibited a decreasing trend for secondary and conjugated BAs in the low LP06CC2–dose group. Moreover, a high LP06CC2 dose caused excess accumulation of Lactobacillales and failed to increase intestinal ZO-1 and occludin expression, while the fecal butyrate level increased dose dependently in the LP06CC2-fed mice. Finally, an appropriate LP06CC2 dose protected the intestinal barrier function from the HFD and modulated BA metabolism.
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Role of Extracellular Vesicles in Absorption and Functional Mechanisms of Quercetin 査読あり
Ishisaka A., Sugimoto R., Marumo H., Doi T., Hamada K., Fujimoto M., Fujiwara N., Yamasaki M., Murakami A.
Molecular Nutrition and Food Research 67 ( 21 ) e2300225 2023年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Molecular Nutrition and Food Research
Scope: Quercetin (QUE), a phytochemical found in various plant foods, has been shown to have a variety of physiological activities in vivo, though biological sites where it has activities and the mechanisms of transport have not been fully elucidated. Methods and results: In the present study, intracellular uptake of QUE into HT-29 human colon adenocarcinoma cells is found to result in spontaneous release of extracellular vesicles (EVs), which are subsequently embedded with QUE. In addition, QUE-embedded EVs are detected in serum of QUE-administered Sprague-Dawley rats. Interestingly, the rate of cellular uptake of QUE-encapsulated EVs (EV-QUE) into RAW264.7 macrophages is markedly higher than that of free QUE. Moreover, EV-QUE suppresses lipopolysaccharide (LPS)-induced nitric oxide at a lower concentration than free QUE. Conclusion: The present findings suggest that QUE may be embedded in EVs in the gastrointestinal tract, then become absorbed and enter the bloodstream to exhibit biological activities.
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Ogawa Kenjirou, Karitani Takara, Otsu Wataru, Nishiyama Kazuo, Kunitake Hisato, Goto Yo, Nomiyama Shota, Hara Hideaki, Yamasaki Masao
BPB Reports 6 ( 3 ) 87 - 97 2023年5月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本薬学会
<i>Background</i>: Blue light causes retinal photoreceptor damage via oxidative and endoplasmic reticulum (ER) stress. A previous study showed that blueberry stem extract (BStEx) and its active components have cytoprotective effects against blue-light-induced photoreceptor cell damage by suppressing oxidative stress. This study demonstrated the inhibitory effect of BStEx against blue light-induced ER stress in photoreceptor cells. <i>Methods</i>: The photoreceptor cells treated with BStEx or the antioxidant <i>N</i>-Acetyl-L-cysteine (NAC) as a positive control were used and then exposed to blue light. The cytoprotective effects of BStEx and NAC were evaluated using CCK-8. The ER stress-related protein expression changes over time, and its levels were measured after each exposure time to blue light in photoreceptor cells treated with BStEx or NAC. <i>Results</i>: BStEx and NAC showed protective effects against blue-light-induced photoreceptor morphological abnormalities and cell damage. Although blue light triggered ER stress factors such as BiP, PERK, ATF6, eIF2α, ATF4, and CHOP, which in turn stimulated cell cycle arrest factors p53 and p21 and upregulation of apoptosis-inducing factors caspase-3. However, BStEx suppressed the increase in expression of BiP, ATF4, ATF6, CHOP, p53, p21, and caspase-3, but not mitochondrial apoptotic factors Bax and cytochrome c. Furthermore, the antioxidant NAC showed similar suppressive effects on BStEx. <i>Conclusion</i>: Our findings suggest that blue light-induced ER stress is primarily caused by oxidative stress, and BStEx might suppress ER stress via an antioxidant effect. The antioxidant NAC contributes to the cell proliferative capacity and suppression of apoptosis in photoreceptor cells.
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Ogawa K., Ohno Y., Tagashira A., Urata K., Satoh K., Fujimoto N., Sonoda H., Ikeda M., Matsuzaki T., Nishiyama K., Kunitake H., Goto Y., Yamasaki M.
In Vivo 37 ( 3 ) 1003 - 1015 2023年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：In Vivo
Background/Aim: Tears secreted from the lacrimal gland are essential for preserving the ocular surface. Thus, dysfunction of the lacrimal gland in Sjögren’s syndrome (SS) can lead to dry eye, resulting in a reduced quality of life. We previously reported that blueberry ‘leaf’ water extract prevents lacrimal hyposecretion in male non-obese diabetic (NOD) mice in a SS-like model. In this study, we investigated the effect of blueberry ‘stem’ water extract (BStEx) on lacrimal hyposecretion in NOD mice. Materials and Methods: Male NOD mice were fed 1% BStEx or control (AIN-93G) for 2, 4, or 6 weeks from 4 weeks of age. Pilocarpine-induced tear secretion was measured using a phenol red-impregnated thread. The lacrimal glands were histologically evaluated by HE staining. Inflammatory cytokine levels in the lacrimal glands were measured using ELISA. Immunostaining was performed to examine aquaporin 5 (AQP5) localization. The expression levels of autophagy-related proteins, AQP5, and phosphorylated AMPK were measured using western blotting. Results: After feeding BStEx to mice for 4 or 6 weeks, tear volume was observed to have increased in the BStEx group compared with that in the control group. There were no significant differences in inflammatory cell infiltration, autophagy-related protein expression, or the localization and expression of AQP5 in the lacrimal glands between the two groups. In contrast, AMPK phosphorylation increased in the BStEx group. Conclusion: BStEx prevented lacrimal hyposecretion in the SS-like model of male NOD mice, probably by opening tight junctions via the activation of AMPK in lacrimal acinar cells.
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Toyama Y., Fujita Y., Toshima S., Hirano T., Yamasaki M., Kunitake H.
Plants 12 ( 4 ) 2023年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plants
Polyphenol-rich rabbiteye blueberry (Vaccinium virgatum Aiton) leaves have attracted attention as a food material. In this study, we compared the total polyphenols, total proanthocyanidin content, and antioxidant activity of the leaves of 18 blueberry varieties and investigated the seasonal variation in polyphenols. We also evaluated the anti-cancer cell proliferation properties of the rabbiteye blueberry leaf specific cultivar ‘Kunisato 35 Gou’. Rabbiteye blueberry leaves had significantly higher total polyphenol and total proanthocyanidin values than northern highbush blueberry and southern highbush blueberry leaves. The antioxidant activity of blueberry leaves was highly positively correlated with both the total polyphenol and total proanthocyanidin content. Variations were observed in the total polyphenol and total proanthocyanidin content of rabbiteye blueberry leaves harvested at different points in the growing season; leaves collected in fall to winter contained more epicatechin in addition to proanthocyanidins. In the evaluation of anti-cancer cell proliferation properties against HL-60 promyelocytic leukemia cells, the September-harvested extracts of rabbiteye blueberry ‘Kunisato 35 Gou’ showed strong properties, and the use of an FITC Annexin V apoptosis detection kit with propidium iodide confirmed that this HL-60 cell death occurred via apoptosis. Limiting the harvest time would make rabbiteye blueberry leaves a more functional food ingredient.
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Blueberry stem extract and stem active components prevent blue light-emitting diode light-induced retinal photoreceptor cell damage in vitro 査読あり
Kenjirou Ogawa, Karin Urata, Yosuke Suzuki, Kazuhiro Sugamoto, Yo Goto, Takayuki Nakayama, Kazuo Nishiyama, Hisato Kunitake, Masao Yamasaki
Bioscience, Biotechnology, and Biochemistry 2023年1月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1093/bbb/zbad001
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Ogawa K., Urata K., Maeda S., Ohno Y., Satoh K., Yamada Y., Suzuki Y., Koga Y., Sugamoto K., Kawaguchi M., Kunitake H., Nishiyama K., Goto Y., Nakayama T., Yamasaki M.
In Vivo 37 ( 1 ) 149 - 162 2023年1月
担当区分：最終著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：In Vivo
Background/Aim: This study evaluated the effect of blueberry leaf hot water extract (BLEx) on Sjögren's syndrome (SS)-like lacrimal hyposecretion in male non-obese diabetic (NOD) mice. Materials and Methods: NOD or BALB/c mice were fed 1% BLEx or control (AIN-93G) for 2 weeks from the age of 4 to 6 weeks. Pilocarpine-induced tear volume was measured using a phenol red-impregnated thread. The lacrimal glands were evaluated histologically by H&E staining. The IL-1β and TNF-α levels in the lacrimal gland tissue were measured by ELISA. The mRNA expression levels of secretion-related proteins were measured by real-time PCR. LC3 I/II and arginase 1 expression levels were measured by western blot. Results: After feeding with BLEx, pilocarpine-induced tear secretion in NOD mice was increased. In contrast, the mRNA expression levels of the cholinergic muscarinic M3 receptor, aquaporin 5, and ion channels related to lacrimal secretion were not changed by BLEx administration. In addition, the protein expression of arginase 1, which was recently reported to be involved in tear hyposecretion in NOD mice, was also not improved by BLEx administration. Although infiltration in the lacrimal gland of NOD mice was not decreased, the levels of TNF-α and the autophagy-related protein LC3 were significantly suppressed by BLEx treatment. Conclusion: BLEx treatment may ameliorate lacrimal hyposecretion in NOD mice by delaying the progression of autoimmune disease by suppressing autophagy in lacrimal glands.
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Autophagy in adipogenesis: Molecular mechanisms and regulation by bioactive compounds 査読あり 国際共著
Khan F, Khan H, Khan A, Yamasaki M, Moustaid-Moussa N, Al-Harrasi A, Rahman SM.
Biomed Pharmacother 113715 2022年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Kai H., Okada Y., Goto Y., Nakayama T., Sugamoto K., Ogawa K., Yamasaki M., Morishita K., Matsuno K., Kunitake H.
Plants 11 ( 10 ) 2022年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plants
Although Vaccinium virgatum Aiton leaves and stems inhibit adult T-cell leukemia (ATL) cells, leaves and stems can differ between individual plants and by time and location. In this study, leaf and stem components were profiled in the same individual plant using direct-injection electron ionization-mass spectrometry (DI-EI-MS) metabolomics, with the aims of analyzing the anti-ATL activity, and quantifying proanthocyanidins (PACs). Leaves, stems, and leaf/stem mixtures showed distinct and characteristic spectra. Anti-ATL activity was stronger in stems than leaves, and the PAC content was higher in stems than leaves. These data were subjected to bivariate analysis to identify the factor (m/z) responsible for the inhibitory effect of ATL based on the highest coefficient of determination (R2 ). The results of this DI-EI-MS metabolomics analysis suggest that among PACs contained in V. virgatum stems and leaves, the fragment ion at m/z 149 contributes significantly to anti-ATL activity.
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Yamasaki M., Kiue Y., Fujii K., Sushida M., Yamasaki Y., Sugamoto K., Suzuki Y., Koga Y., Kunitake H., Kai H., Ogawa K., Nishiyama K., Goto Y., Nakayama T.
Plants 10 ( 12 ) 2021年12月
担当区分：筆頭著者, 責任著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plants
Blueberry (Vaccinium virgatum Aiton; Kinisato 35 Gou) leaves have recently attracted in-creasing attention as a useful material for the prevention of lifestyle diseases. Here, we examined the effects of the hot water extract of blueberry leaves (BLEx) on lipogenesis and uric acid production in 3T3-L1 adipocytes. The results showed that BLEx suppressed lipid accumulation and the mRNA expression of differentiation markers in 3T3-L1 adipocytes. A fractionation study showed that the highly polymerized proanthocyanidin-rich fraction was responsible for this effect. Upon maturation to adipocytes, 3T3-L1 cells produced uric acid and tumor necrosis factor-α, and hypoxia stimulated the production of uric acid and xanthine oxidoreductase activity. BLEx suppressed the production of uric acid under these conditions. Although BLEx inhibited the enzymatic activity of xanthine oxidase, this activity was observed in several fractions containing catechin, epicatechin, chlorogenic acid, rutin, and low molecular weight proanthocyanidins. Taken together, these results indicate that BLEx contains various compounds with the ability to suppress lipid accumulation and uric acid production in adipocytes.
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Enhanced water dispersibility and permeability through a Caco-2 cell monolayer of β-cryptoxanthin extracted from kumquats by complexation with casein 査読あり
Ohima T, Takanashi K, Inada A, Yamasaki M, Yamasaki Y, Eto N
Food Chemistry 360 129822 2021年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, however, Their Intracellular Incorporation by Endocytosis Was Not Involved in this Effect on RAW264 Cells. 査読あり
Yamasaki M, Yamasaki Y, Furusho R, Kimura H, Kamei I, Sonoda H, Ikeda M, Oshima T, Ogawa K, Nishiyama K
Molecules 26 ( 9 ) 2763 2021年5月
担当区分：筆頭著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Role of macrophage autophagy in atherosclerosis: modulation by bioactive compounds. 査読あり
Zahid MK, Sufian HB, Choudhury M, Yamasaki M, Al-Harrasi A, Moustaid-Moussa N, Rahman SM
Biochemical Journal 478 1359 - 1375 2021年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1042/BCJ20200894
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地域素材を活用した高機能性食品の開発 -宮崎完熟きんかん「たまたま」を使用した加工特性解析と加工品の開発-
山﨑 有美, 坂元 亜美, 河野 愛未, 大島 達也, 髙橋 幸毅, 篠原 有紀子, 福井 敬一, 吉山 佳世, 近藤 知巳, 山﨑 正夫
宮崎大学地域資源創成学部紀要 ( 4 ) 129 - 142 2021年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：宮崎大学地域資源創成学部
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Toyama Y., Toshima S., Hirano T., Yamasaki M., Kunitake H.
Journal of Berry Research 11 ( 4 ) 689 - 704 2021年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Berry Research
BACKGROUND: In blueberries, the total polyphenol contents, total anthocyanin contents, and antioxidant activities of fruits in blueberries and their relatives are becoming important targets for breeders. Recently, intersectional hybrids between Japanese wild species Shashanbo and highbush blueberry were produced using polyploid breeding for the first time. However, the polyphenol contents, antioxidant activities, and anti-cancer cell proliferation properties of the fruits of these intersectional hybrids have not been studied sufficiently. OBJECTIVE: The objectives of this study were to determine the anthocyanin contents, polyphenol contents, and antioxidant activities at each stage of fruit development in intersectional hybrids, and to examine the effects of the fruit extracts on anti-cancer cell proliferation properties using human promyelocytic leukemia (HL-60) cells. METHODS: Freeze-dried fruit samples of three intersectional hybrids and their parents at five developmental stages were used to evaluate their polyphenol contents, anthocyanin contents, and levels of two types of antioxidant activities (DPPH and ORAC). The anti-cancer cell proliferation properties of each extract were also evaluated according to the survival rate of HL-60 cells. RESULTS: The total polyphenol contents in fruits of the intersectional hybrids gradually decreased as the fruits grew, but the contents increased slightly at the mature stage. Meanwhile, the total anthocyanin contents increased strongly to the mature stage. In addition, the fruit extract of intersectional hybrid JM1 was found to be more effective for inhibiting the growth of HL-60 human leukemia cells in vitro. Total polyphenol content correlated highly with antioxidant activities and anti-cancer cell proliferation properties. CONCLUSIONS: We clarified that the intersectional hybrids had produced have abundant bioactive compounds, high levels of antioxidant activities, and anti-cancer cell proliferation properties. The results proved the effectiveness of the use of Japanese wild Vaccinium species for the strategic breeding of blueberries with high levels of bioactive compounds.
DOI： 10.3233/JBR-210713
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Yamasaki M., Minesaki M., Iwakiri A., Miyamoto Y., Ogawa K., Nishiyama K., Tsend-Ayush C., Oyunsuren T., Li Y., Nakano T., Takeshita M., Arima Y.
Food Science and Nutrition 8 ( 11 ) 6164 - 6173 2020年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Food Science and Nutrition
© 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC Previous study suggested that dietary intake of Lactobacillus plantarum 06CC2 (LP06CC2) isolated from Mongolian dairy products showed various health beneficial effects. Here, the effect of LP06CC2 on the cholesterol metabolism in mice fed a cholesterol-loaded diet was evaluated. Cholesterol and LP06CC2 were incorporated into the AIN93G-based diet to evaluate the effect on cholesterol metabolism in Balb/c mice. Serum and liver cholesterol levels were significantly increased in mice fed a cholesterol-loaded diet whereas the LP06CC2 ingestion suppressed the increase of liver cholesterol. LP06CC2 suppressed the increase of the hepatic damage indices. The increase of the cecal content and fecal butyrate were observed in mice fed LP06CC2. The analysis of bile acids clearly showed that LP06CC2 increased their deconjugation indicating the decrease of bile acid absorption. The protein expression of hepatic Cyp7A1 was also suppressed by LP06CC2 in mice fed cholesterol. Finally, in vitro studies showed that LP06CC2 had the most potent ability to deconjugate bile acids using glycocholate among the tested probiotic lactic acid bacteria isolated from Mongolian dairy products. Taken together, LP06CC2 is a promising microorganism for the reduction of the cholesterol pool via modulation of bile acid deconjugation.
DOI： 10.1002/fsn3.1909
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奄美島豚の肉質評価に関する研究 査読あり
叶内宏明、 山﨑正夫、鞍掛文、木村郁夫、大塚彰
鹿児島大学紀要 70 1 - 6 2020年3月
記述言語：日本語 掲載種別：研究論文（大学，研究機関等紀要）
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Shoji K., Yamasaki M., Kunitake H.
Journal of Oleo Science 69 ( 2 ) 143 - 151 2020年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oleo Science
©2020 by Japan Oil Chemists’ Society. Prevention of postprandial hypertriglyceridemia is an important consideration for reducing the risk of developing cardiovascular disease. While blueberry fruits have been reported to ameliorate lipid metabolism in humans, there are only few research reports on the effects of blueberry leaves (BL). Here, we investigated the efficacy of BL on postprandial hyperlipidemia in subjects with high fasting triacylglycerol (TG) concentrations. Randomized, double-blind, cross-over design study was conducted. The subjects consumed a BL containing beverage or a placebo beverage before a fat-enriched test meal. Blood samples were collected prior to and 1, 2, 3, 4, and 5 hours after consuming the test beverage. The postprandial serum TG and remnant-like particle cholesterol (RLP-C) concentrations were significantly lower in the BL beverage compared with those in the placebo beverage. Additionally, BL was more effective in subjects with high fasting ghrelin with gastric emptying function. In current study, fasting ghrelin correlated with the increase in postprandial serum TG, suggesting that BL ameliorates hypertriglyceridemia through delayed gastric emptying. In conclusion, this pilot study suggests that BL may be useful as an early dietary therapy for treating postprandial hyperlipidemia.
DOI： 10.5650/jos.ess19201
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エタノール・アセトアルデヒド併用処理によるアルコール性肝細胞毒性の誘導効果と酸化ストレスとの関係
山﨑 楓, 西山 和夫, 山﨑 正夫
日本醸造協会誌 115 ( 7 ) 419 - 428 2020年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益財団法人 日本醸造協会
本研究は，エタノールとアセトアルデヒドの併用処理による肝細胞への影響とそのメカニズムを解明することが目的である。ラット正常肝細胞において，エタノールとアセトアルデヒドを併用処理すると，生細胞数の有意な減少，G0/G1期細胞の割合の有意な増加，G2/M期細胞の割合の有意な減少が確認された。さらに，細胞内ROS量の有意な増加も確認された。このことから，エタノールとアセトアルデヒドの共存によって酸化ストレス，細胞周期の遅延を介して，肝細胞毒性が誘導されることが示唆された。しかし，細胞内ROS量の増加においては，エタノール単独処理でも同レベルの変化が確認されたことから，エタノールとアセトアルデヒドの共存下における肝細胞毒性の増強には，細胞内ROS量は関与していないが，アセトアルデヒドが細胞内ROS量以外の部分で補助的に作用している可能性がある。また，エタノール・アセトアルデヒド併用処理による細胞内ROS量の増加に対して，CYP2E1の阻害剤を処理すると，有意に改善させたが，NADPHオキシダーゼの阻害剤を処理すると変化しなかったことから，エタノール・アセトアルデヒド併用処理による酸化ストレスの誘導は，CYP2E1の活性が関与していることが示唆された。以上のことから，エタノールとアセトアルデヒドの併用処理は，酸化ストレスの誘導と細胞周期の遅延を介して，アルコール性肝細胞毒性を誘導することが示唆された。そして，エタノール・アセトアルデヒド併用処理による酸化ストレスの誘導は，CYP2E1によるものであることが分かった。
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Kosakai T., Kato H., Sho C., Kawano K., Iwai K., Takase Y., Ogawa K., Nishiyama K., Yamasaki M.
PeerJ 2019 ( 9 ) 2019年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：PeerJ
Copyright © 2019 Kosakai et al. It has been reported that fermented products (FPs) prepared from sweet potatoshochu distillery by-product suppressed weight gain and decreased serum cholesterol levels in mice under normal dietary conditions. Furthermore, from the information gained from the above data regarding health benefits of the FPs, the aim of this study was evaluating the effects of dietary FPs on lipid accumulation and gut microbiota in mice with or without cholesterol-load in the diet. C57BL/6N mice were fed normal (CO) diet, CO with 10% FPs (CO + FPs) diet, cholesterol loaded (HC) diet, or HC with 10% FPs (HC + FPs) diet for 8 weeks. The mice were then euthanized, and blood samples, tissue samples, and feces were collected. The adipose tissue weight and liver triglyceride levels in the HC + FPs diet groups were significantly reduced compared to that in the HC diet groups. However, FPs significantly increased the serum non-high-density lipoprotein cholesterol (HDL-C) levels, the ratio of non-HDL-C to HDL-C and hepatic total cholesterol levels in mice fed cholesterolloaded diet compared with that of the HC diet group. Since dietary FPs significantly decreased the protein expression levels of cholesterol 7 alpha-hydroxylase 1 in the HC + FPs diet groups, the cholesterol accumulation in FPs group may be explained by insufficient catabolism from cholesterol to bile acid. In addition, the dietary FPs tended to increase Clostridium cluster IV and XIVa, which are butyrate-producing bacteria. Related to the result, n-butyrate was significantly increased in the CO + FPs and the HC + FPs diet groups compared to their respective control groups. These findings suggested that dietary FPs modulated the lipid pool and gut microbiota.
DOI： 10.7717/peerj.7671
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Inada A., Oue T., Yamashita S., Yamasaki M., Oshima T., Matsuyama H.
European Journal of Pharmaceutical Sciences 136 2019年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：European Journal of Pharmaceutical Sciences
© 2019 Elsevier B.V. The water-solubility of coenzyme Q10 (CoQ10) is extremely low because of its hydrophobicity, which results in low bioavailability. In this study, peptide mixtures derived from different proteins (casein, albumin, gelatin, lysozyme, zein and hemoglobin) were prepared and used as dispersants ofCoQ10. Most peptide mixtures, except for that derived from lysozyme, enhanced the water-dispersibility of CoQ10 by forming complexes with CoQ10. In particular, the apparent solubility of CoQ10complexed with the casein hydrolysate (Pepcas) was the highest. Pepcas was fractionated by ammonium sulfate precipitation and ultrafiltration to find the most effective peptides for enhancing water-dispersibility of CoQ10. The peptide fraction that had a relatively hydrophobic nature and peptides of higher-than-average molecular weights (PepcasA) was found to be the most effective. Results from differential thermal analysis and powder X-ray diffraction revealed that the complex between CoQ10 and PepcasA in the solid state was amorphous. In solution, the complex is a hydrocolloid with particle sizes of 154–279 nm.
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Novel fermented products made from sweet potato-shochu distillery by-products reduces body fat and serum cholesterol in mice. 査読あり
T. Kosakai, R. Nobetsu, C. Sho, K. Kawano, K. Iwai, Y. Takase, K. Nishiyama, M. Yamasaki
Journal of The Brewing Society of Japan 114 ( 5 ) 294 - 301 2019年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Chronic intake of high-dose of blueberry leaf extract does not augment the harmful effects of ethanol in rats. 査読あり
K. Yamasaki, K. Sugamoto, T. Arakawa, K. Nishiyama, M. Yamasaki
PeerJ 2019年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Blueberry Leaf Polyphenols Prevent Body Fat Accumulation in Mice Fed High-fat, High-sucrose Diet
Fujii K., Ota Y., Nishiyama K., Kunitake H., Yamasaki Y., Tari H., Araki K., Arakawa T., Yamasaki M.
Journal of oleo science 68 ( 5 ) 471 - 479 2019年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of oleo science
Blueberry leaf is currently a popular dietary supplement. Effects of dietary blueberry leaf and its active components on body fat accumulation were examined. C57BL/6J mice were fed high-fat, high-sucrose diet with or without 3% blueberry leaf extract (BLEx) or 3% concentrated-polyphenolic BLEx (CP BLEx) for 8 weeks. Compared to mice fed a high-fat, high-sucrose diet without blueberry leaf, BLEx and CP BLEx significantly reduced body weight and adipose tissue weight gain. Adipocytes were also smaller and and liver lipid accumulatioin was significantly inhibited in mice fed either BLEx or CP BLEx. These effects tended to be more pronounced in mice fed CP BLEx compared to in mice fed BLEx. Together, results suggest that blueberry leaf inhibits body fat accumulation typically observed in mice fed a high-fat, high-sucrose diet, and that inhibition is attributable to polyphenolic components in leaf extracts.
DOI： 10.5650/jos.ess18226
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Yamasaki M., Hamada K., Fujii K., Nishiyama K., Yamasaki Y., Tari H., Araki K., Arakawa T.
Biochemistry and Biophysics Reports 14 182 - 187 2018年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochemistry and Biophysics Reports
© 2018 The Authors Blueberry (Vaccinium ashei) leaf has recently attracted attention as an anti-obesity food component. In this study, we examined the effects of blueberry leaf extract (BLEx) on insulin signaling in C2C12 differentiated myoblasts. The results showed that BLEx promotes the intracellular uptake of 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]−2-deoxy-D-glucose (2-NBDG) and phosphorylation of Akt under insulin stimulation. In addition, pretreatment with BLEx ameliorated TNF-α-nduced insulin resistance with regard to 2-NBDG uptake and Akt phosphorylation. Moreover, BLEx prevented the TNF-α−induced activation of JNK and NF-kB pathways and phosphorylation of IRS-1 at serine residue. BLEx failed to induce phosphorylation of AMPK as well as did not prevent the restoration of 2-NBDG uptake under TNF-α–induced insulin resistance. Overall, skeletal muscle is a putative target for the anti-diabetic effect of BLEx by amelioration of insulin resistance.
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Dietary borage oil promotes gamma-linoleic acid accumulation in dextran sulfate sodium-treated mice but does not manipulate the severity of colitis 査読あり
R. Fujioka, K. Nishiyama, M. Yamasaki
Journal of Food and Nutrition Research 5 ( 3 ) 151 - 155 2017年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Nakanishi T., Tokunaga Y., Yamasaki M., Erickson L., Kawahara S.
Animal Science Journal 87 ( 12 ) 1554 - 1561 2016年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Animal Science Journal
© 2016 Japanese Society of Animal Science Conjugated linoleic acid (CLA) is one of the constituents of animal products with possible health benefits such as anti-carcinogenic and anti-obesity effects. In this study, we investigated the immunomodulatory effects of CLA using a mouse model of allergic dermatitis. Mice were orally administered either a CLA mixture containing equal amounts of 9c, 11 t-CLA and 10 t, 12c-CLA, or high linoleic acid safflower oil, and allergic dermatitis was induced on the ear by repeated topical applications of oxazolone. Oral administration of the CLA mixture but not the high linoleic safflower oil attenuated the symptoms of allergic dermatitis in both ear weights and clinical scores. This effect was associated with decreased levels of ear interleukin-4 (IL-4) and plasma immunoglobulin E. The immunomodulatory effects of the CLA isomers were compared by an in vitro cytokine production assay. The results showed that 9c, 11 t-CLA, the most predominant isomer in animal products, significantly inhibited IL-4 and interferon-γ production from mouse splenocytes with similar potency to 10 t, 12c-CLA. These findings suggest that CLA, a constituent of animal products, has a potentially beneficial effect for amelioration of allergic dermatitis.
DOI： 10.1111/asj.12603
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共役脂肪酸の多様な機能性とその利用 臨床利用への足掛かり 招待あり 査読あり
木下和昭、野見山将太、山崎正夫
日本農芸化学会誌「化学と生物」 2016年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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A single dose of blueberry leaf extract suppresses serum ethanol levels after oral ethanol administration in rats. 査読あり
Yamasaki K, Nagatomo H, Kawamura Y, Sugamoto K, Kai T, Kamenaga K, Takeshita M, Kikuchi Y, Matsuura Y, Yukizaki C, Nishiyama K, Yamasaki M
European Journal of Scientific Research 2016年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Preparation of conjugated linoleic acid nanoemulsions and their biodistribution. 査読あり
K. Kishita, K. Ibaraki, S. Itakura, Y. Yamasaki, N. Nishikata, K. Yamamoto, M. Shimizu, K. Nishiyama, M. Yamasaki
Journal of Oleo Science 65 ( 11 ) 949 - 954 2016年
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Blueberry (Vaccinium virgatum Aiton) leaf extract ameliorates insulin resistance in mice fed a high-fat, high-sucrose diet. 査読あり
Yamasaki M., Matsuyama Y., Hayasegawa R., Hamada K., Nishiyama K., Kai T., Kamenaga K., Arakawa T., Tari H., Shimizu Y., Matsuura Y., Yukizaki C
Food Science and Technology Research 21 ( 6 ) 827 - 833 2015年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Nano-emulsions of conjugated linoleic acid suppresses inflammatory response in macrophage but not in experimental inflammatory bowel disease condition. 査読あり
M. Yamasaki, H. Kanazaki, R. Fujioka, K. Kishita, K. Nishiyama, K. Yamamoto, S. Hamayama, M. Shimizu
European Journal of. Scientific Research 2015年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Imamura K., Asai M., Sugamoto K., Matsumoto T., Yamasaki Y., Kamei I., Hattori T., Kishimoto M., Niisaka S., Kubo M., Nishiyama K., Yamasaki M.
Bioscience, Biotechnology and Biochemistry 79 ( 6 ) 1021 - 1025 2015年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
© 2015 Japan Society for Bioscience, Biotechnology, and Agrochemistry. In this study, we focused on the anti-inflammatory effect of cordycepin, 3′-deoxyadenosine. Cordycepin potently suppressed nitric oxide production in lipo-polysaccharide-stimulated RAW 264.7 cells in an adenosine receptor-independent manner. In addition, inhibitors for adenosine kinase and nucleoside transporter abrogated the action of cordycepin. Thus, we considered that intracellular metabolism cordycepin is important for the anti-inflammatory effect of cordycepin.
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Yamasaki M., Nishimura M., Sakakibara Y., Suiko M., Morishita K., Nishiyama K.
Food and Function 5 ( 11 ) 2842 - 2849 2014年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Food and Function
© 2014 the Partner Organisations. Here, we examined the effect of tocotrienols (T3) on the growth of adult T-cell leukemia (ATL) cells. All three forms (β-, γ-, and δ-T3) inhibited cell proliferation in a dose-dependent manner; δ-T3 showed the strongest growth-inhibitory effect. δ-T3 increased the G1, G2/M, and subG1 populations and induced internucleosomal DNA fragmentation. δ-T3 treatment also increased the levels of cleaved caspase-3, -6, -7, -9, and poly-ADP ribose polymerase (PARP), and this was accompanied by downregulation of Bcl-2, Bcl-xL, and XIAP. Moreover, δ-T3 decreased nuclear p65 NF-κB levels, indicating downregulation of NF-κB activity. This cytotoxic effect of δ-T3 was abrogated by squalene (SQL) but not mevalonate (MVL), farnesyl diphosphate (FPP), geranylgeranyl diphosphate (GGPP), or cholesterol (CL). δ-T3 decreased intracellular SQL levels, and inhibition of de novo cholesterol synthesis did not affect the action of SQL. Furthermore, δ-T3 significantly decreased farnesyl-diphosphate farnesyltransferase 1 (FDFT1) expression. Taken together, it is evident that δ-T3, due to its ability to potently induce apoptosis via the depletion of intracellular SQL, shows the potential to be considered a therapeutic agent in patients with ATL.
DOI： 10.1039/c4fo00635f
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The importance of 1,2-dithiolane structure in α-lipolic acid for the downregulation of cell surface β1-integrin expression of human bladder cancer cells. 査読あり
M. Yamasaki, S. Soda, Y. Sakakibara, M. Suiko, K. Nishiyama
Bioscience Biotechnology Biochemistry 78 ( 11 ) 1939 - 1942 2014年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Ishida Y., Yamasaki M., Yukizaki C., Nishiyama K., Tsubouchi H., Okayama A., Kataoka H.
Human Cell 27 ( 2 ) 68 - 77 2014年4月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Human Cell
Adult T-cell leukemia/lymphoma (ATL) is a fatal malignancy caused by infection with human T-lymphotropic virus type-1 and there is no accepted curative therapy for ATL. We searched for biological active substances for the prevention and treatment of ATL from several species of herbs. The ATL cell growth-inhibitory activity and apoptosis assay showed that carnosol, which is an ingredient contained in rosemary (Rosmarinus officinalis), induced apoptosis in ATL cells. Next, to investigate the apoptosis-inducing mechanism of carnosol, we applied proteomic analysis using fluorescent two-dimensional differential gel electrophoresis and mass spectrometry. The proteomic analysis showed that the expression of reductases, enzymes in glycolytic pathway, and enzymes in pentose phosphate pathway was increased in carnosol-treated cells, compared with untreated cells. These results suggested that carnosol affected the redox status in the cells. Further, the quantitative analysis of glutathione, which plays the central role for the maintenance of intracellular redox status, indicated that carnosol caused the decrease of glutathione in the cells. Further, N-acetyl-l-cystein, which is precursor of glutathione, canceled the efficiency of carnosol. From these results, it was suggested that the apoptosis-inducing activity of carnosol in ATL cells was caused by the depletion of glutathione. © 2013 Japan Human Cell Society and Springer Japan.
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Identification of a novel flavonoid glycoside sulfotransferase in Arabidopsis thaliana. 査読あり
Hashiguchi T, Sakakibara Y, Shimohira T, Kurogi K, Yamasaki M, Nishiyama K, Akashi R, Liu MC, Suiko M.
Journal of Biochemistry 155 ( 2 ) 91 - 97 2014年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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α-Lipoic acid suppresses migration and invasion via downregulation of cell surface β1-integrin expression in bladder cancer cells. 査読あり
Yamasaki M, Iwase M, Kawano K, Sakakibara Y, Suiko M, Ikeda M, Nishiyama K.
Journal of Clinical Biochemistry and Nutrition 54 ( 1 ) 18 - 25 2014年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Induction of apoptotic cell death in HL-60 cells by jacaranda seed oil derived fatty acids
Yamasaki M., Motonaga C., Yokoyama M., Ikezaki A., Kakihara T., Hayasegawa R., Yamasaki K., Sakono M., Sakakibara Y., Suiko M., Nishiyama K.
Journal of Oleo Science 62 ( 11 ) 925 - 932 2013年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oleo Science
Various fatty acids are attracting considerable interest for their anticancer effects. Among them, fatty acids containing conjugated double bonds show one of the most potent cytotoxic effects on cancer cells. Here, we focused on the cancer cell killing activity of jacaranda seed oil. The seed oil of jacaranda harvested from Miyazaki in Japan contained 30.9% cis-8, trans-10, cis-12 octadecatrienoic acid, called jacaric acid (JA). Fatty acid prepared from this oil (JFA) and JA strongly induced cell death in human leukemia HL-60 cells. On the other hand, linoleic acid and trans-10, cis-12 conjugated linoleic acid ( < 10 μM) did not affect cell proliferation and viability. An increase in the sub-G 1 population and internucleosomal fragmentation of DNA was observed in JA- and JFA-treated cells, indicating induction of apoptotic cell death. Finally, the cytotoxic effects of JA and JFA were completely abolished by α-tocopherol. Taken together, these data suggest that jacaranda seed oil has potent apoptotic activity in HL-60 cells through induction of oxidative stress. © 2013 by Japan Oil Chemists' Society.
DOI： 10.5650/jos.62.925
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山崎 正夫
日本栄養・食糧学会誌 : Nippon eiy◆U014D◆ shokury◆U014D◆ gakkaishi = Journal of Japanese Society of Nutrition and Food Science 66 ( 5 ) 241 - 247 2013年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本栄養・食糧学会
共役リノール酸を始めとする共役脂肪酸は, 天然界においては比較的まれな脂肪酸であるが, その極めて強い生理機能が細胞, 動物レベルで示されてきた。このような機能性は私たちの健康に大いに貢献できる可能性を秘めており, 共役脂肪酸は機能性食品素材の候補として期待される。著者らは共役脂肪酸の機能性食品への応用を目指し, その体内動態を解析するため, ガスクロマトグラフィーによる分析法の確立を目的とした前処理方法を確立した。さらに, 細胞試験および動物試験により機能性の探索を進め, 共役脂肪酸は脾臓や腸間膜リンパ節リンパ球の抗体産生増強効果や, アポトーシス誘導による肝ガン細胞の致死活性があることを明らかとした。これらの作用は共役リノール酸の異性体により活性が大きく異なり, 共存するモノ不飽和脂肪酸によって機能性が影響を受けることを示した。本総説では共役リノール酸を中心とするこれらの機能性に関する知見を紹介したい。
DOI： 10.4327/jsnfs.66.241
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Inhibitionof cell motility by fucoidan derived from Cladosiphon okamuranus
Yamasaki Y., Ando Y., Yamasaki M., Tachibana H., Yamada K.
Nippon Shokuhin Kagaku Kogaku Kaishi 60 ( 7 ) 357 - 361 2013年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nippon Shokuhin Kagaku Kogaku Kaishi
Fucoidanis anactive component of seaweed that has beenshownto exhibit a number of biological regulation effects, such as inhibition of proliferation and induction of apoptosis, invarious tumor cells. Inthis study, we demonstrated that fucoidanisolated from Cladosiphon okamuranus significantly inhibited cell motility in several cancer cells in a dose-dependent manner. Fucoidan induced the disruption of stress fibers at concentrations greater than 10 μg/mL. However, fucoidanwas not shownto decrease the phosphorylationof the myosinII regulatory light chain(MRLC) at Thr18/Ser19 inB16 cells. These data suggested that the inhibition of cell motility by treatment with fucoidan was induced via a pathway other than MRLC.
DOI： 10.3136/nskkk.60.357
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Genistein induces apoptotic cell death associated with inhibition of the NF-kB pathway in adult T-cell leukemia cells 査読あり
M. Yamasaki, Y. Mine, M. Nishinura, S. Fujita, Y. Sakakibara, M. Suiko, K.Morishita, K. Nishiyama.
Cell Biology international 37 ( 7 ) 742 - 747 2013年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Adipocyte response to conjugated linoleic acid (Review) 査読あり
M. Yamasaki, T. Yanagita
Obesity Research & Clinical Practice 7 e235 - e242 2013年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Jacaric acid is rapidly metabolized to conjugated linoleic acid in rats
Kijima R., Honma T., Ito J., Yamasaki M., Ikezaki A., Motonaga C., Nishiyama K., Tsuduki T.
Journal of Oleo Science 62 ( 5 ) 305 - 312 2013年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oleo Science
We have shown previously that jacaric acid (JA; 8c,10t,12c-18:3), which has a conjugated triene system, has a strong anti-tumor effect. However, the characteristics of absorption and metabolism of JA have yet to be determined in vivo, and the details of absorption and metabolism of JA in the small intestine are particularly unclear. This information is required for effective use of JA in humans. Therefore, in this study we examined absorption and metabolism of JA using cannulation of the thoracic duct in rats. Emulsions of two test oils, jacaranda seed oil and tung oil, which contain JA and α-eleostearic acid (α-ESA; 9c,11t,13t-18:3), respectively, were administered to rats and lymph from the thoracic duct was collected over 24 h. We examined the rate of absorption of JA and possible conversion to a conjugated linoleic acid (CLA)containing a conjugated diene system. The positional isomerism of the CLA produced by JA metabolism was determined using gas chromatography-electron impact/mass spectrometry. The rate of absorption and percentage conversion of JA were compared with those of α-ESA. We found that JA is rapidly absorbed and converted to a CLA in rats and that the percentage conversion of JA was lower than that of α-ESA. This is the first report on the absorption and metabolism of JA and this information may be important for application of JA as a functional food. ©2013 by Japan Oil Chemists' Society.
DOI： 10.5650/jos.62.305
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Tsuda H., Kunitake H., Kawasaki-Takaki R., Nishiyama K., Yamasaki M., Komatsu H., Yukizaki C.
Plants 2 ( 1 ) 57 - 71 2013年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plants
© 2013 by the authors; licensee MDPI, Basel, Switzerland. Antioxidants are abundant in blueberries, and while there are many studies concerning the bioactive compound of fruit, it is only recently that the wild Vaccinium species has attracted attention for their diverse and abundant chemical components. The aim of this study was to investigate the bioactive compounds of blueberry cultivars and wild species found in Japan. Among the five extracts of the Vaccinium species, Natsuhaze (Vaccinium oldhamii Miq.) was found to be the most effective at inhibiting the growth of HL-60 human leukemia cells in vitro. Although all ethanol extracts showed a growth inhibitory effect on HL-60 cells, the degree of the effects differed among the species. The extract of Natsuhaze induced apoptotic bodies and nucleosomal DNA fragmentation in the HL-60 cells. Of the extracts tested, that of Natsuhaze contained the largest amount of total polyphenols and showed the greatest antioxidant activity, but the anthocyanin content of Natsuhaze was similar to that of rabbiteye blueberry (V. virgatum Ait.). The results showed that total polyphenols contributed to the high antioxidant activity and growth inhibitory effect on HL-60 human leukemia cells of Natsuhaze extract.
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Yamasaki M., Mine Y., Nishimura M., Fujita S., Sakakibara Y., Suiko M., Morishita K., Nishiyama K.
Cell Biology International 37 ( 7 ) 742 - 747 2013年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cell Biology International
We have shown that genistein inhibits the growth of adult T-cell leukemia (ATL) cells in vitro and in vivo, and this leads to pronounced G2/M arrest. This report shows that genistein induces apoptotic death in ATL cells. Although the pan-caspase inhibitor, Z-VAD-fmk, did not inhibit genistein-induced apoptosis, release of apoptosis-inducing factor (AIF) into the cytosol occurred. Poly-ADP ribose polymerase inhibition also abrogated genistein-induced apoptosis. Genistein decreased nuclear p65 translocation and IkBα phosphorylation, and downregulated the anti-apoptotic proteins, XIAP, cIAP and survivin, NF-kBresponsive gene products. Thus, genistein is a promising agent for ATL that induces caspase-independent apoptosis through inhibition of the NF-kB pathway. © 2013 International Federation for Cell Biology.
DOI： 10.1002/cbin.10101
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Shinohara N., Ito J., Tsuduki T., Honma T., Kijima R., Sugawara S., Arai T., Yamasaki M., Ikezaki A., Yokoyama M., Nishiyama K., Nakagawa K., Miyazawa T., Ikeda I.
Journal of Oleo Science 61 ( 8 ) 433 - 441 2012年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oleo Science
Conjugated fatty acid is a collective term used for fatty acids with conjugated double bond systems. Seed oils from certain plants include conjugated linolenic acids, which have a conjugated triene system and are geometrical and positional isomers of α-linolenic acid. One of these isomers, jacaric acid (JA, 8c, 10t, 12c-18:3), has not been examined widely. Therefore, we investigated the absorption and metabolism of JA in normal animals (ICR mice). An oral dose of JA of 5 mg/day for 1 week had no effects on body weight, food intake and tissue weight of mice. JA was detected in the serum, kidney, liver, lung and epididymal white adipose tissue. Analysis of the fatty acid composition in liver and white adipose tissue showed a tendency to increase levels of saturated fatty acids (SFAs) such as palmitic acid (16:0) and stearic acid (18:0) and to decrease levels of monounsaturated fatty acids (MUFAs) such as palmitoleic acid (16:1) and oleic acid (18:1). Thus, JA treatment decreased the desaturation index (16:1/16:0, 18:1/18:0) in liver and white adipose tissue. This index is used as an indicator of the activity of stearoyl coenzyme A desaturase (SCD), an endoplasmic reticulum enzyme that catalyzes the biosynthesis of MUFAs from SFAs. The change in this index indicates that JA inhibited SCD activity in ICR mice, and further experiments showed that JA also decreased the expression level of SCD-1 mRNA. Inhibition of SCD activity may have anti-obesity and anti-diabetes effects, and therefore the findings in this study suggest that JA may be effective for preventing obesity and diabetes. ©2012 by Japan Oil Chemists' Society.
DOI： 10.5650/jos.61.433
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Conjugated linoleic acids inhibit hypoxia inducible factor-1alpha stabilization under hypoxic condition in human hepatocellular carcinoma cells. 査読あり
M. Yamasaki, T Nagatomo, T Matsuyama, Y Ikeho, E Kato, K Nishiyama, Y. Sakakibara, M Suiko, K Nishiyama
Journal of oleo science 61 ( 9 ) 491 - 496 2012年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Jacaric acid, a linolenic acid isomer with a conjugated triene system, has a strong antitumor effect in vitro and in vivo. 査読あり
N Shinohara, T Tsuduki, J Ito, T Honma, R Kijima, S Sugawara, T Arai, M Yamasaki, A Ikezaki, M Yokoyama, K Nishiyama, K Nakagawa, T Miyazawa, I Ikeda.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 1821 ( 7 ) 980 - 988 2012年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Important role of β 1-integrin in fucoidan-induced apoptosis via caspase-8 activation
Yamasaki Y., Yamasaki M., Tachibana H., Yamada K.
Bioscience, Biotechnology and Biochemistry 76 ( 6 ) 1163 - 1168 2012年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Fucoidan induces apoptosis by activating caspase-8 in human MCF-7 breast cancer cells, but the detailed mechanism for this is not understood. We demonstrate here that fucoidan interacted with the cell surface, and silencing the β 1 -integrin gene expression inhibited fucoidan-induced apoptosis accompanied by caspase-8 activation. Fucoidan induced formation of the β 1 -integrin-caspase-8 complex. These data indicate that β 1 -integrin is an important factor for the cell-surface binding of fucoidan and plays an important role in fucoidan-induced apoptosis. Fucoidan also induced recruitment of caspase-8 to the β 1 -integrin intracellular domain, cleaved it into the activated protein by direct combination with β 1 -integrin, and induced apoptosis via the caspase cascade in MCF-7 cells.
DOI： 10.1271/bbb.111001
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Yamasaki M., Iwase M., Kawano K., Sakakibara Y., Suiko M., Nishiyama K.
Bioscience, Biotechnology and Biochemistry 76 ( 6 ) 1177 - 1181 2012年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
We determined the effects of apocynin, a representative inhibitor of NADPH oxidase, on the proliferative and adhesive properties of 3Y1 rat fibroblasts and the 3Y1 v-H-ras-transformed derivative, HR-3Y1-2. Apocynin inhibited the proliferation of HR-3Y1-2 but not 3Y1 cells at 10μM and 100μM. Apocynin also decreased the intracellular reactive oxygen species (ROS) level in HR-3Y1-2 but not 3Y1 cells. We also evaluated the effects of apocynin on cell adhesion to fibronectin and found decreased adhesion of HR-3Y1-2 cells to fibronectincoated plates. Our results indicate that apocynin selectively down-regulated β1-integrin cell surface expression on the HR-3Y1-2 cells. It also inhibited the migration and invasion of these cells. These data suggest that reducing the production of NADPH oxidasemediated ROS could be an effective means for ameliorating the abnormal growth, adhesion and motility of v-H-ras-transformed cells.
DOI： 10.1271/bbb.120061
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Effect of highly lipolyzed goat cheese on HL-60 human leukemia cells: antiproliferative activity and induction of apoptotic DNA damage. 査読あり
asuda, S., Kuwata, H., Kawamoto, K., Shirakawa, J., Hoshi, J., Yamasaki M., Nishiyama K., Tachibana,H., Yamada, K., Kobayashi, H., Igoshi, K.
Journal of Dairy Science 95 ( 5 ) 2248 - 2260 2012年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Alpha Lipoic Acid Selectively Inhibits Proliferation and Adhesion to Fibronectin of v-H-ras-transformed 3Y1 Cells. 査読あり
M. Yamasaki, M. Iwase, K. Kawano, Sakakibara, M. Suiko, and K. Nishiyama.
Journal of Clinical Biochemistry and Nutrition 50 ( 3 ) 234 - 240 2012年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Enzymatic sulfation of tocopherols and tocopherol metabolites by human cytosolic sulfotransferases
Hashiguchi T., Kurogi K., Sakakibara Y., Yamasaki M., Nishiyama K., Yasuda S., Liu M., Suiko M.
Bioscience, Biotechnology and Biochemistry 75 ( 10 ) 1951 - 1956 2011年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Tocopherols are essential micronutrients for mammals widely known as potent lipid-soluble antioxidants that are present in cell membranes. Recent studies have demonstrated that most of the carboxychromanol (CEHC), a tocopherol metabolite, in the plasma exists primarily in sulfate- and glucuronide- conjugated forms. To gain insight into the enzymatic sulfation of tocopherols and their metabolites, a systematic investigation was performed using all 14 known human cytosolic sulfotransferases (SULTs). The results showed that the members of the SULT1 family displayed stronger sulfating activities toward tocopherols and their metabolites. These enzymes showed a substrate preference for γ-tocopherol over α-tocopherol and for γ-CEHC over other CEHCs. Using A549 human lung epithelial cells in a metabolic labeling study, a similar trend in the sulfation of tocopherols and CEHCs was observed. Collectively, the results obtained indicate that SULTmediated enzymatic sulfation of tocopherols and their metabolites is a significant pathway for regulation of the homeostasis and physiological functions of these important compounds.
DOI： 10.1271/bbb.110352
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榊原 陽一, 中原 幸太, 森永 浩通, 山崎 正夫, 西山 和夫, 水光 正仁
生物工学会誌 : seibutsu-kogaku kaishi 89 ( 10 ) 593 - 596 2011年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本生物工学会
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Novel screening method for potential skin-whitening compounds by a luciferase reporter assay
Shirasugi I., Sakakibara Y., Yamasaki M., Nishiyama K., Matsui T., Liu M., Suiko M.
Bioscience, Biotechnology and Biochemistry 74 ( 11 ) 2253 - 2258 2010年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Measurement of the melanin content by using B16 melanoma cells is generally applied to find novel skinwhitening agents. However, this measurement method using B16 melanoma cells has such disadvantages, as the time taken, its sensitivity, and troublesomeness. We therefore attempted in the present study to establish a reporter assay system by measuring the tyrosinase promoter activity to use for convenient, high-throughput screening of new melanogenesis inhibitors. We first confirmed the validity of this reporter assay system by using such known skin-whitening agents, as arbutin, sulforaphane, and theaflavin 3,3'-digallate. We then compared the effect of 56 compounds on the tyrosinase promoter activity to test this reporter assay system. Carnosol, and rottlerin strongly inhibited the tyrosinase promoter activity. Moreover, carnosol and rottlerin decreased melanin synthesis and tyrosinase expression in a dose-dependent manner when using B16 melanoma cells. These results indicate this new luciferase reported assay system to be an effective and convenient method for screening potential skin-whitening compounds.
DOI： 10.1271/bbb.100466
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Genistein induced apoptotic cell death in adult t-cell leukemia cells through estrogen receptors
Yamasaki M., Mukai A., Ohba M., Mine Y., Sakakibara Y., Suiko M., Morishita K., Nishiyama K.
Bioscience, Biotechnology and Biochemistry 74 ( 10 ) 2113 - 2115 2010年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Adult T-cell leukemia (ATL) occurs in human T-lymphotropic virus type I-infected individuals and is endemic to the southwestern area of Kyushu in Japan. Here, we found that nM levels of genistein and 17-estradiol had cytotoxic effects on ATL cells and activated caspase-3. The estrogen receptor antagonist ICI182780 negated the cytotoxic effect of genistein. In addition, G protein-coupled estrogen receptor agonist G-1 also had a cytotoxic effect on ATL cells. This is the first report suggesting that estrogen receptors are a molecular target for ATL therapy.
DOI： 10.1271/bbb.100359
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山崎 正夫, 西山 和夫
バイオサイエンスとインダストリー = Bioscience & industry 68 ( 5 ) 332 - 335 2010年9月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：バイオインダストリー協会
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Effects of highly ripened cheeses on HL-60 human leukemia cells: antiproliferative activity and induction of apoptotic DNA damage. 査読あり
Yasuda S, Ohkura N, Suzuki K, Yamasaki M, Nishiyama K, Kobayashi H, Hoshi Y, Kadooka Y, Igoshi K.
Journal of Dairy Science 93 ( 4 ) 1393 - 1400 2010年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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スイス留学体験記
山崎正夫
生物機能研究会誌 2009年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Omi Y., Fujii N., Ozaki A., Nakama A., Sakakibara Y., Suiko M., Nishiyama K.
Bioscience, Biotechnology and Biochemistry 73 ( 10 ) 2217 - 2221 2009年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Cruciferous vegetables and their isothiocyanates are promising foods and agents for cancer prevention. We focus here on the effects of mustard oil (SMO) in a variety of the Japanese radish, Shibori Daikon (Raphanus sativus), on the proliferation of 3Y1 rat fibroblasts and the H-ras-transformed derivative, HR-3Y1-2. SMO (1.6 μ, g/ml) inhibited the proliferation of HR-3Y1-2, but not 3Y1 after 24h after treatment. A cell cycle analysis showed that SMO induced G2/M arrest after 6 h, although this effect was not observed 24 h after the treatment. SMO transiently decreased the cellular reduced glutathione level accompanied with up-regulation of the intracellular reactive oxygen species 2-3 h post-treatment. Glutathione ethyl ester and N- acetyl-L-cysteine prevented the growth inhibitory effect of SMO. This mustard oil extract consisted of 95.6% 4-methylthio-3-butenyl isothiocyanate and 4.4% 4-methylthiobutyl isothiocyanate. SMO selectively inhibited H-ras-transformed 3Y1 cells associated with transient oxidative stress via reduced glutathione (GSH) depletion.
DOI： 10.1271/bbb.90322
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Fucoidan induces apoptosis through activation of caspase-8 on human breast cancer MCF-7 cells
Yamasaki-Miyamoto Y., Yamasaki M., Tachibana H., Yamada K.
Journal of Agricultural and Food Chemistry 57 ( 18 ) 8677 - 8682 2009年9月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Agricultural and Food Chemistry
Fucoidan is an active component of seaweed that has been shown to inhibit proliferation and induce apoptotic cell death in several tumor cells. However, the detailed mechanisms underlying this process have not yet been elucidated. In the present report, we investigated the effect of fucoidan on the induction of apoptosis in human breast cancer MCF-7 cells. Our data demonstrated that fucoidan reduced the viable cell number of MCF-7 cells in a dose- and time-dependent manner. In contrast, fucoidan did not affect the viable cell number of normal human mammary epithelial cells. Results from the apoptosis assay demonstrated that fucoidan induced internucleosomal DNA fragmentation, chromatin condensation, activation of caspase-7, -8, and -9, and cleavage of poly(ADP ribose) polymerase. Furthermore, expression of Bid was decreased, whereas truncated Bid was increased by fucoidan treatment. There was also a decline in cytosolic Bax and a striking increase of cytosolic cytochrome c. Caspase-8-specific inhibitor, z-ITED-fmk, canceled the cytotoxicity of fucoidan, activation of caspase-7, -8, and -9, and a series of changes in Bax, Bid, and cytochrome c. However, caspase-9-specific inhibitor exerted a moderate inhibitory effect on the cytotoxicity of fucoidan. These data indicated that fucoidan could induce apoptotic cell death through a caspase-8-dependent pathway in MCF-7 cells. © 2009 American Chemical Society.
DOI： 10.1021/jf9010406
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Dihydro-alpha-lipoic acid has more potent cytotoxicity than alpha-lipoic acid. 査読あり
M. Yamasaki, A. Kawabe, K. Nishimoto, H. Madhyastha, Y. Sakakibara, M. Suiko, T. Okamoto, T. Suda, K. Uehira, and K. Nishiyama.
In Vitro Cellular Develomental Biology-Animal 45 ( 5-6 ) 275 - 280 2009年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Tachibana H., Yamada A., Ochi Y., Madhyastha H., Nishiyama K., Yamada K.
In Vitro Cellular and Developmental Biology - Animal 44 ( 7 ) 290 - 294 2008年8月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：In Vitro Cellular and Developmental Biology - Animal
Our previous study indicated that oleic acid prevented apoptotic cell death induced by trans10, cis12 (t10, c12)-conjugated linoleic acid in rat hepatoma dRLh-84 cells. The intracellular mechanism of action oleic acid is still unknown. Here, we showed that p38 mitogen-activated protein kinase (MAPK) inhibition using its specific inhibitor SB203580 cancelled the ameliorative effect of oleic acid on the cytotoxicity of t10, c12-conjugated linoleic acid. In addition, SubG1 cell population analysis showed that p38 MAPK played an essential role in the prevention of apoptotic cell death by oleic acid. In fact, p38 phosphorylation level was upregulated in cells treated with oleic acid irrespective of t10, c12-conjugated linoleic acid stimulation. Interestingly, t10, c12-conjugated linoleic acid increased intracellular triglyceride accumulation. However, oleic acid completely inhibited this effect. These observations indicated the involvement of blockade of a p38 MAPK pathway in the ameliorative effect of oleic acid on apoptosis induced by t10, c12-conjugated linoleic acid. © 2008 The Society for In Vitro Biology.
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抗変異原性活性をもつ宮崎県産農産物の探索 査読あり
岩田 喬子、榊原 陽一、赤松 絵奈、酒井 美穂、柚木崎 千鶴子、山崎 正夫、西山 和夫、水光 正仁
宮崎大学農学部研究報告 54 69 - 76 2008年1月
記述言語：日本語 掲載種別：研究論文（大学，研究機関等紀要）
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Induction of endoreduplication by a JNK inhibitor SP600125 in human lung carcinoma A549 cells. 査読あり
Y. Miyamoto-Yamasaki, M. Yamasaki, H. Tachibana, and K. Yamada.
Cell Biology International 31 ( 12 ) 1501 - 1506 2007年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Soy-derived isoflavones inhibit the growth of adult T-cell leukemia cells in vitro and in vivo
YAMASAKI Masao, FUJITA Satoshi, ISHIYAMA Erina, MUKAI Ayako, MADHYASTHA Harishkumar, SAKAKIBARA Yoichi, SUIKO Masahito, HATAKEYAMA Kinta, NEMOTO Takayuki, MORISHITA Kazuhiro, KATAOKA Hiroaki, TSUBOUCHI Hirohito, NISHIYAMA Kazuo
Cancer science 98 ( 11 ) 1740 - 1746 2007年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Reduced expression of perchloric acid-soluble protein after partial hepatectomy in rats
Kanouchi H., Taga M., Okamoto T., Yamasaki M., Oka T., Yamada K., Tone S., Minatogawa Y.
Bioscience, Biotechnology and Biochemistry 70 ( 1 ) 290 - 292 2006年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
We examined the expression of perchloric acid-soluble protein (PSP) during liver regeneration after partial hepatectomy (PH) in rats. Liver regeneration was almost complete at 7-d after PH. Expression of PSP protein and mRNA decreased and then gradually increased during liver regeneration. An immunohistochemical study showed that PSP is distributed in cytosol and nuclei in normal liver, but localization in the nuclei was not be recognized in the regenerated liver.
DOI： 10.1271/bbb.70.290
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Dietary effect of pomegranate seed oil on immune function and lipid metabolism in mice. 査読あり
M. Yamasaki, T. Kitagawa, H. Chujo, N. Koyanagi, H. Maeda, J. Kohno-Murase, J. Imamura, H. Tachibana, and K. Yamada
Nutrition 22 ( 1 ) 54 - 59 2006年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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リポ酸による白血病細胞HL-60のアポトーシス誘導効果 招待あり
山崎正夫、山根理学、西本健太朗、榊原陽一、水光正仁、西山和夫
Food Function 2 ( 1 ) 1 - 5 2006年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Miyamoto Y., Chujo H., Nishiyama K., Tachibana H., Yamada K.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 1735 ( 3 ) 176 - 184 2005年8月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Conjugated linoleic acid (CLA) is a powerful anti-carcinogenic fatty acid. Previously, we showed that 10trans 12cis (10t, 12c) CLA induced apoptotic cell death in rat hepatoma. Here, we demonstrated significant cytotoxic effects of 1 μM 10t, 12c-CLA, but not 9c, 11t-CLA, on dRLh-84 rat hepatoma cells. 9t, 11t and 9c, 11c-CLA also showed low levels of cytotoxic activity. 10t, 12c-CLA activated caspase-3, 9 followed by cytochrome c release from mitochondria into the cytosol. Inhibitors of caspase-3, 9 blocked the cytotoxicity of 10t, 12c-CLA. 10t, 12c-CLA also induced translocation of Bax protein into the mitochondrial membrane and cleavage of Bid protein. Lysosomal destabilization induced by 10t, 12c-CLA was observed by monitoring the re-localization of Acridine Orange and the leakage of β-hexosaminidase from lysosomes. 10t, 12c-CLA directly degraded the isolated lysosomes from the rat liver. Our observations indicate that 10t, 12c-CLA induces mitochondria-related apoptosis accompanied by lysosomal destabilization in rat hepatoma cells. © 2005 Elsevier B.V. All rights reserved.
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Cytotoxity of the trans10, cis12 isomer of conjugated linoleic acid on rat hepatoma and its modulation by other fatty acids and tocopherol and tocotrienol. 査読あり
M. Yamasaki, E. Nishida, S. Nou, H. Tachibana, K. Yamada.
In Vitro Cellular Develomental Biology-Animal 41 ( 7 ) 239 - 244 2005年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Nakaya M., Yamasaki M., Tachibana H., Yamada K.
Immunology Letters 98 ( 2 ) 225 - 231 2005年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Immunology Letters
Estrogens have diverse effects on cell growth, differentiation and homeostatic functions, and have been shown to play an important role in regulating immune system. In this study, we examined the effect of 17β-estradiol (E2) on antibody production by splenocytes isolated from C57BL/6N mice. Our results suggest that the activation of immunoglobulin (Ig) M production by E2 requires direct cell-cell interaction between adherent and non-adherent cells in mouse splenocyte population, and the primary target of E2 is adherent cell population. In addition, we indicated that ER antagonist ICI 182780 suppressed this enhancing effect of E2. Both ERα agonist and ERα agonist enhanced IgM production of mouse splenocytes. ERs are expressed on plasma membrane as well as in nucleus. However, a plasma membrane-associated ER specific ligand has no stimulation effect on IgM production. In conclusion, our results indicate that adherent cells stimulated by E2 up-regulate IgM production of lymphocytes through the direct cell-cell interactions, and the enhancing effect of E2 is arouse through ERα and ERβ on these cells. © 2004 Elsevier B.V. All rights reserved.
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Gamma-linoleic acid(GLA)による肝再生促進効果に関する研究 査読あり
辻田英司, 島田光生、武冨紹信、祇園智信、調憲、田中真二、伊藤心二、山崎正夫、山田耕路、前原喜彦
外科と代謝・栄養 38 ( 3 ) 119 2004年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Kitagawa T., Chujo H., Koyanagi N., Nishida E., Nakaya M., Yoshimi K., Maeda H., Nou S., Iwata T., Ogita K., Tachibana H., Yamada K.
Journal of Agricultural and Food Chemistry 52 ( 11 ) 3644 - 3648 2004年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Agricultural and Food Chemistry
Previous studies have shown the physiological significance of dietary conjugated linoleic acid (CLA) in various experimental animals and in human beings. One of the important problems to better elucidate is the difference between triglyceride (TG) and free (FFA) dietary CLA. Here, using splenocytes, this study assesses how TG- and FFA-CLA modulate immunoglobulin and various cytokine productions. In this study, C57BL/6N mice were fed an experimental diet containing 0% CLA, 0.1 or 1% FFA-CLA, or 0.1 or 1% TG-CLA for 3 weeks. The production of immunoglobulin tended to be up-regulated by 1% FFA-CLA. As a result of protein array analysis using the supernatant from splenocytes cultured with no CLA, 1% FFA-CLA, and TG-CLA, some cytokine production was shown to be remarkably regulated by dietary FFA- and TG-CLA. A total of 32 cytokines were examined, and 11-14 produced cytokines that were 2-fold up-regulated as compared with control for FFA- or TG-CLA, respectively. Especially, the production of IL-9 and MCP-5 and other cytokines was remarkably up-regulated by both FFA- and TG-CLA. In addition, seven cytokines were 2-fold down-regulated by TG-CLA. These data show that there is a slight but significant difference between the functionalities of FFA- and TG-CLA.
DOI： 10.1021/jf049929j
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Induction of apoptosis by tocotrienol in rat hepatoma dRLh-84 cells. 査読あり
M. Sakai, M. Okabe, M. Yamasaki, H. Tachibana, K. Yamada.
Anticancer Research 24 ( 3a ) 1683 - 1688 2004年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effect of dietary conjugated linoleic acid on liver regeneration after a partial hepatectomy in rats. 査読あり
A. Hirao, M. Yamasaki, H. Chujo, N. Koyanagi, H. Kanouchi, S. Yasuda, A. Matsuo, E. Nishida, E. Tsujita, T. Rikimaru, M. Shimada, Y. Maehara, H. Tachibana and K. Yamada.
Journal of Nutritional Science and Vitaminology 50 ( 1 ) 9 - 12 2004年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Dietary protein modulates the effect of CLA on lipid metabolism in rats 査読あり
A. Akahoshi., K. Koba., F. Ichinose., M. Kaneko., A. Shimoda., K. Nonaka., M. Yamasaki., T. Iwata., Y. Yamauchi., K. Tsutsumi., M. Sugano
Lipids 39 ( 1 ) 25 - 30 2004年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Chujo H., Yamasaki M., Nou S., Koyanagi N., Tachibana H., Yamada K.
Cancer Letters 202 ( 1 ) 81 - 87 2003年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cancer Letters
We evaluated the effect of conjugated linoleic acid (CLA) isomers on the growth factor-induced proliferation of human breast cancer MCF-7 cells. When MCF-7 cells were cultured in RPMI 1640 medium supplemented with 1% fetal bovine serum (FBS), CLA inhibited the proliferation and notably cis9, trans11 (c9,t11)-CLA showed the strongest effect. However, cells barely grew when cultured with 1% charcoal-treated FBS (cFBS). Proliferation was promoted in cFBS cultured cells by the addition of 17β-estradiol (E2), insulin, and epidermal growth factor (EGF). Trans10, cis12 (T10,c12)-CLA inhibited cell proliferation induced by E2 and insulin, but not by EGF. T10,c12-CLA also exhibited cell-killing activity when cells were induced with insulin. On the other hand, c9,t11-CLA was shown to have no effect on MCF-7 cell proliferation induced by and of these three growth factors. In conclusion, although both c9, t11 and t10, c12-CLA can inhibit the proliferation of MCF-7 cells, our results suggested that they have separate mechanisms and different targets of actions. © 2003 Elsevier Ireland Ltd. All rights reserved.
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Estrogenic compounds suppressed interferon-gamma production in mouse splenocytes through direct cell-cell interaction. 査読あり
M. Nakaya, M. Yamasaki, Y. Miyazaki, H. Tachibana, and K. Yamada.
In Vitro Cellular Develomental Biology-Animal 39 ( 8 ) 383 - 387 2003年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Antioxidant and anti-inflammatory effects of a diet supplemented with sesamin on hepatic ischemia-referfusion injury in rats. 査読あり
T. Utsunomiya, M. Shimada, T. Rikimaru, H. Hasegawa, Y. Yamashita, T. Hamatsu, M. Yamasaki, S. Kaku, K. Yamada and K.Sugimachi.
Hepato-Gastroenterology 50 ( 53 ) 1609 - 1613 2003年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Chujo H., Nou S., Tachibana H., Yamada K.
Cancer Letters 196 ( 2 ) 187 - 196 2003年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cancer Letters
We showed the inhibitory effect of oleic and palmitoleic acids (OA and POA) on this trans10, cis12 (10t, 12c)-CLA induced cytotoxic activity. When cells were cultured in the presence of 10t, 12c-CLA, this potent cytotoxic effect on dRLh-84 cells was clearly obvious when compared to the control vehicle group. It was revealed that the levels of cellular OA and POA levels decreased upon cis9, trans11 (9c,11t) or 10t, 12c-CLA treatment in a time course dependent manner. OA or POA demonstrates a dose dependent inhibition of the 10t, 12c-CLA induced cytotoxicity. Notable nuclear fragmentation or activation of caspase-3 and 9 by 10t, 12c-CLA in dRLh-84 was counteracted by treatment with OA or POA. Results also suggest that 10t, 12c-CLA induced apoptosis can be inhibited by treatment with OA or POA. © 2003 Elsevier Science Ltd. All rights reserved.
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共役脂肪酸の免疫調節機能 査読あり
山崎正夫、山田耕路
食品工業 46 ( 6 ) 31 - 35 2003年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Chujo H., Hirao A., Koyanagi N., Okamoto T., Tojo N., Oishi A., Iwata T., Yamauchi-Sato Y., Yamamoto T., Tsutsumi K., Tachibana H., Yamada K.
Journal of Nutrition 133 ( 3 ) 784 - 788 2003年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Nutrition
We evaluated the effect of cis-9, trans-11 (9c, 11t) and trans-10, cis-12 (10t, 12c) conjugated linoleic acid (CLA) on the immune system in C57BL/6J mice. Mice were fed experimental diets containing 0% CLA (controls), 1% 9c, 11t-CLA, 1% 10t, 12c-CLA or a 1:1 mixture (0.5% + 0.5%) of these two CLA isomers for 3 wk. Relative spleen weights of all CLA fed mice were greater than the controls. Spleen lymphocytes isolated from the mice fed 10t, 12c-CLA produced more immunoglobulin (Ig)A and IgM but not IgG when stimulated with concanavalin A (ConA) compared with controls. IgA production from unstimulated spleen lymphocytes was greater in the 10t, 12c-CLA group than in controls. Conversely, 9c, 11t-CLA did not affect the production of any of the Ig subclasses. Lymphocytes isolated from 9c, 11t-CLA fed mice produced more tumor necrosis factor-α than the control group. The proportion of B cells in the spleen lymphocyte population was significantly lower in the 9c, 11t-CLA group, and higher in the 10t, 12c-CLA group than in the controls. Compared with the control group, the percentage of CD4 + T cells was lower in the 10t, 12c-CLA group, and the percentage of CD8 + T cells was higher in the 9c, 11t-CLA group. Furthermore, the percentage of CD8 + T cells was higher in the 1:1 mixture group than in controls. The CD4 + /CD8 + ratio was lower in the 1:1 mixture group than in controls. These results suggest that 9c, 11t and 10t, 12c-CLA can stimulate different immunological effects and that the simultaneous intake of the two isomers can change the T cell population.
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Yamasaki M., Ikeda A., Oji M., Tanaka Y., Hirao A., Kasai M., Iwata T., Tachibana H., Yamada K.
Nutrition 19 ( 1 ) 30 - 35 2003年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nutrition
OBJECTIVES: In this study, we examined the effect of dietary conjugated linoleic acid (CLA) on body fat levels in Sprague-Dawley rats. Methods: Rats were fed AIN-93G type diets containing 4%, 7%, and 10% fats with or without 1.5% CLA. Results: Three weeks after the onset of the experimental period, the weights of perirenal white adipose tissue were lower in CLA-fed rats. The weights of epididymal white adipose tissue also were lower in CLA-fed rats than in control rats, but this effect disappeared with increased dietary fat level. Serum leptin levels tended to be lower in the CLA group, especially the low-fat diet group, than in the control group. There were significant positive correlations between serum leptin level and weights of perirenal and epididymal white adipose tissues in control groups, but these correlations were weaker in the CLA groups. Serum tumor necrosis factor-α levels also tended to be lower in CLA-fed rats, and this tendency was most remarkable in the rats fed 7% fat diets. Conclusions: In conclusion, dietary CLA, especially the low-fat diet, reduced body fat without hepatic injury to Sprague-Dawley rats. © Elsevier Science Inc. 2003.
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Yamasaki M., Chujo H., Koga Y., Oishi A., Rikimaru T., Shimada M., Sugimachi K., Tachibana H., Yamada K.
Cancer Letters 188 ( 1-2 ) 171 - 180 2002年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cancer Letters
We evaluated the cytotoxic effect of conjugated linoleic acid (CLA) on rat hepatoma dRLh-84 cells in vitro. When cells were cultured in the presence of CLA, strong cytotoxic effect on dRLh-84 cells was recognized at 1μM level compared to the control vehicle group, and trans10, cis12-CLA but not cis9, trans11-CLA was shown to be an active isomer for inducing this effect. Increase of the sub-G1 population and activation of caspase-3 and 9 accompanied with a time-dependent cleavage of poly(ADP-ribose) polymerase were recognized in dRLh-84 cells treated with trans10, cis12-CLA. In addition, we could see nuclear fragmentation in dRLh-84 cells treated with trans10, cis12-CLA by laser scanning confocal microscopy observation. Cytotoxic effect of trans10, cis12-CLA on normal hepatocytes was weaker than on dRLh-84 cells. These data indicate trans10, cis12-CLA has a potent cytotoxic effect on dRLh-84 cells through at least in part by an apoptotic pathway. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
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Inhibitory effect of conjugated linoleic acid isomers on the proliferation of human breast cancer cells
H. Chujo, M. Yamasaki, N. Koyanagi, H. Tachibana, and K. Yamada
Animal Cell Technology for creation of new era 13 465 - 470 2002年12月
記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス）
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Dose dependent effect of dietary conjugated linoleic acid on the growth of rat hepatoma dRLh-84 cells in vivo. 査読あり
M. Yamasaki, A. Ikeda, A. Hirao, Y. Tanaka, T. Rikimaru, M. Shimada, K. Sugimachi, H. Tachibana, and K. Yamada.
Journal of Nutritional Science and Vitaminology 48 ( 6 ) 505 - 511 2002年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Conjugated linoleic acid acid reduces body fats and cytokine level of mice. 査読あり
Akahoshi, Y. Goto, K. Murao, T. Miyazaki, M. Yamasaki, M. Nonaka, K. Yamada and M. Sugano
Bioscience Biotechnology Biochemistry 66 ( 4 ) 916 - 920 2002年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Dietary conjugated linolenic acid in relation to CLA differently modifies body fat mass and serum and liver lipid levels in rats. 査読あり
K. Koba, A. Akahoshi, M. Yamasaki, K. Tanaka, K. Yamada, T. Iwata, T. Kamegai, K. Tsutsumi, and M. Sugano.
Lipids 37 ( 4 ) 343 - 350 2002年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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共役リノール酸の免疫調節効果 招待あり
山崎正夫、山田耕路
日本食品化学工学会誌 49 ( 2 ) 130 - 134 2002年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Dietary manipulations of body fat-reducing potential of conjugated linoleic acid in rats 査読あり
M. Sugano, A. Akahoshi, K. Koba, K. Tanaka, T. Okumura, H. Matsuyama, Y. Goto, T. Miyazaki, K. Murao, M. Yamasaki, M. Nonaka, and Koji Yamada.
Bioscience Biotechnology Biochemistry 2001年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Oxidative stress by visible right irradiation suppresses immunoglobulin production in mouse spleen lymphocytes. 査読あり
Y. Miyazaki, M. Yamasaki, H. Mishima, K. Mansho, H. Tachibana, and K. Yamada
Bioscience Biotechnology Biochemistry 2001年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effect of dietary conjugated linoleic acid on the in vivo growth of rat hepatoma dRLh-84
Yamasaki M., Ikeda A., Hirao A., Tanaka Y., Miyazaki Y., Rikimaru T., Shimada M., Sugimachi K., Tachibana H., Yamada K.
Nutrition and Cancer 40 ( 2 ) 140 - 148 2001年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nutrition and Cancer
We examined the effect of dietary conjugated linoleic acid (CLA) on the growth of injected hepatoma dRLh-84 in Donryu rats. After experimental diets containing 0% or 2% CLA were given to male Donryu rats for 3 wk, dRLh-84 cells were injected into the left lobe of the hepatic capsule, and the experimental diet was continued. The cells formed a solid tumor ≥1 wk after the injection, and thereafter the tumor grew with feeding duration. In a morphological study, this tumor appeared to be a low-differentiated hepatoma, and there was no remarkable difference in the morphology of the tumor between 0% and 2% CLA groups. Tumor weight was significantly higher in the 2% CLA group than in the 0% CLA group throughout the feeding period after the injection. Serum glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase activities were significantly higher in 2% CLA-injected rats than in 0% CLA-injected rats at 3 wk after the injection. CLA upregulated acyl-CoA oxidase activity, especially 1 wk after the injection. However, dietary CLA did not activate carnitine palmitoyl transferase II, which is a rate-limiting enzyme in the mitochondrial β-oxidation pathway. Natural killer cell activity in the spleen tended to be higher in injected rats, but a significant effect of dietary CLA was not recognized. Serum interferon-γ and tumor necrosis factor-α levels were higher in injected than in sham rats. More-over, these levels were higher in 2% CLA groups than in the respective 0% CLA groups.
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Acute reduction of serum leptin level by dietary conjugated linoleic acid in Sprague-Dawley rats
Yamasaki M., Mansho K., Ogino Y., Kasai M., Tachibana H., Yamada K.
Journal of Nutritional Biochemistry 11 ( 9 ) 467 - 471 2000年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Nutritional Biochemistry
The purpose of this study was to clarify the effect of conjugated linoleic acid on lipid accumulation in adipose tissue. Sprague-Dawley rats were fed a diet containing 2% conjugated linoleic acid for 1, 3, 6, and 12 weeks. In rats fed 2% conjugated linoleic acid, the weight of perirenal white adipose tissue was comparable with that of rats fed a conjugated linoleic acid-free diet. For fatty acid composition of perirenal white adipose tissue, both 16:1/16:0 and 18:1/18:0 ratios were significantly lower in the conjugated linoleic acid-fed group than the control group. Although there was no remarkable difference in serum triglyceride, total cholesterol, and phospholipid levels between dietary groups, serum leptin level was significantly lower than the control group, and lipid content in the perirenal white adipose tissue exerted a tendency toward low compared to the control value at 1-week feeding. On the other hand, leptin level in perirenal white adipose tissue was significantly lower in the conjugated linoleic acid-fed group than the control group at 12-week feeding. In conclusion, these observations suggest dietary conjugated linoleic acid is an acute reducer of serum leptin level. This may afford an explanation of the mechanism of anti-obesity effect in conjugated linoleic acid. (C) Elsevier Science Inc. 2000.
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Effect of dietary conjugated linoleic acid on lipid peroxidation and histological change in rat liver 査読あり
M. Yamasaki, K. Mansho, H. Mishima, G. Kimura, M. Sasaki, M. Kasai, H. Tachibana, and K. Yamada
Journal of Agricultural and Food Chemistry 2000年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Regulation of serum leptin level by dietary conjugated linoleic acid.
M. Yamasaki, K. Mansho, Y. Ogino, M. Kasai, H. Tachibana, and K. Yamada.
Animal Cell Technology for creation of new era 12 467 - 472 2000年12月
記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス）
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Dietary conjugated linoleic acid increases immunoglobulin productivity of Sprague-Dawley rat spleen lymphocytes 査読あり
M. Yamasaki, K. Kishihara, K. Mansho, Y. Ogino, M. Kasai, M. Sugano, H. Tachibana and K. Yamada
Bioscience Biotechnology Biochemistry 2000年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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A recommended esterification method for gas chromatographic measurement of conjugated linoleic acid. 査読あり
M. Yamasaki, K. Kishihara, I. Ikeda, M. Sugano, and K. Yamada
Journal of American Oil Chemists Society 1999年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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O-412 肝虚血再灌流障害 : Nutritional immunomodulationの応用 査読あり
宇都宮 徹、島田 光生、祇園 智信、長谷川 博文、山崎 正夫、加来 志保子、山田 耕路、杉町 圭蔵
日本外科学会雑誌 100 210 1999年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Mansho K., Mishima H., Kasai M., Sugano M., Tachibana H., Yamada K.
Bioscience, Biotechnology and Biochemistry 63 ( 6 ) 1104 - 1106 1999年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
We examined the effect of dietary conjugated linoleic acid (CLA) on lipid parameters in the liver, white adipose tissue (WAT) and brown adipose tissue (BAT) of Sprague-Dawley rats and found that it reduced the levels of triglycerides and non-esterified fatty acid in the liver and WAT without significant change in the BAT lipid levels. These results suggest that CLA has an obesity-preventing action. © 1999, Taylor & Francis Group, LLC. All rights reserved.
DOI： 10.1271/bbb.63.1104
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Conjugated linoleic acid modulates tissue levels of chemical mediators and immunoglobulins in rats
Sugano M., Tsujita A., Yamasaki M., Noguchi M., Yamada K.
Lipids 33 ( 5 ) 521 - 527 1998年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Lipids
The effects of conjugated linoleic acid (CLA) on the levels of chemical mediators in peritoneal exudate cells, spleen and lung, and the concentration of immunoglobulins in mesenteric lymph node and splenic lymphocytes and in serum were examined in rats. After feeding diets containing either 0 (control), 0.5 or 1.0% CLA for 3 wk, there was a trend toward a reduction in the release of leukotriene B 4 (LTB 4 ) from the exudate cells in response to the dietary CLA levels. However, CLA did not appear to affect the release of histamine. A similar dose-response pattern also was observed in splenic LTB 4 , lung LTC 4 and serum prostaglandin E 2 levels, and the differences in these indices between the control and 1.0% CLA groups were all statistically significant. The reduction by CLA of the proportions of n-6 polyunsaturated fatty acids in peritoneal exudate cells and splenic lymphocyte total lipids seems to be responsible at least in part for the reduced eicosanoid levels. Splenic levels of immunoglobulin A (IgA), IgG, and IgM increased while those of IgE decreased significantly in animals fed the 1.0% CLA diet. This was reflected in the serum levels of immunoglobulins. The levels of IgA, IgG, and IgM in mesenteric lymph node lymphocytes increased in a dose-dependent manner, while IgE was reduced in those fed the higher CLA intake. However, no differences were seen in the proportion of T-lymphocyte subsets of mesenteric lymph node. These results support the view that CLA mitigates the food- induced allergic reaction.
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Lymphatic recovery, tissue distribution, and metabolic effects of conjugated linoleic acid in rats. 査読あり
M. Sugano, A. Tsujita, M. Yamasaki, K. Yamada, I. Ikeda and D. Kritchevsky
Journal of Nutritional Biochemistry 1997年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）