論文 - 山﨑 正夫
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食品中のナノ粒子に関する機能性研究 査読あり
山﨑正夫、山﨑有美、大島達也
化学と生物 61 ( 2 ) 78 - 84 2023年2月
担当区分：筆頭著者, 責任著者 記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Nanovesicles derived from edible plants: a new player that contributes to the function of foods
Yamasaki M., Yamasaki Y., Oshima T.
Bioscience, Biotechnology and Biochemistry 89 ( 2 ) 179 - 186 2025年2月
担当区分：筆頭著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Nano-sized vesicles are ubiquitous in vegetables, fruits, and other edible plants. We have successfully prepared nanovesicles (NVs) from over 150 edible plants. These results suggest that the daily intake of NVs from various foods and their roles in food function are promising novel approaches for explaining the health-promoting properties of edible plants. These vesicles contain RNAs, including miRNAs, similar to extracellular NVs, which play pivotal roles in cell-cell communication. Intriguingly, NVs also contain phytochemicals such as polyphenols and carotenoids that are specific to each edible plant. In conclusion, these dietary NVs have the potential to serve as functional packages to deliver RNAs or phytochemicals to target cells across species from plants to humans.
DOI： 10.1093/bbb/zbae198
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Nei S., Matsusaki T., Kawakubo H., Ogawa K., Nishiyama K., Tsend-Ayush C., Nakano T., Takeshita M., Shinyama T., Yamasaki M.
Nutrients 16 ( 17 ) 2024年9月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nutrients
ATP-binding cassette transporter subfamily G member 2 (ABCG2) is responsible for the excretion of foreign substances, such as uric acid (UA) and indoxyl sulfate (IS), from the body. Given the importance of increased ABCG2 expression in UA excretion, we investigated the enhancement of intestinal ABCG2 expression using Lactiplantibacillus plantarum 06CC2 (LP06CC2). Mice were reared on a potassium oxonate-induced high-purine model at doses of 0.02% or 0.1% LP06CC2 for three weeks. Results showed that LP06CC2 feeding resulted in increased ABCG2 expression in the small intestine. The expression level of large intestinal ABCG2 also showed a tendency to increase, suggesting upregulation of the intestinal excretion transporter ABCG2 by LP06CC2. Overall, LP06CC2 treatment increased fecal UA excretion and showed a trend towards increased fecal excretion of IS, suggesting that LP06CC2 treatment enhanced the expression of intestinal ABCG2, thereby promoting the excretion of UA and other substances from the intestinal tract.
DOI： 10.3390/nu16173042
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Takeguchi T., Kai H., Takeshita M., Miura M., Ogawa K., Nishiyama K., Yamasaki M.
Journal of Oleo Science 73 ( 7 ) 991 - 999 2024年7月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oleo Science
In this study, we evaluated the cancer cell killing activity of koji mold-derived extracts using several solvents. The koji mold lipid extract (KML) exhibited potent cytotoxicity against a human leukemia cell line. Fractionation of the KML via silica gel chromatography revealed the presence of active components in fraction (Fr.) 6. Cytotoxic effects of Fr. 6 were inhibited by the ferroptosis inhibitors, ferrostatin-1 and SRS11-92, and the iron chelator, deferoxamine. Interestingly, ferroptosis inhibitors failed to prevent the KML-induced cell death. Fr. 6 decreased the expression of glutathione peroxidase 4 (GPx4) and increased the level of peroxidized plasma membrane lipids. Furthermore, Fr. 6 decreased the intracellular glutathione levels. Overall, our results suggest that Fr. 6 included in KML induces ferroptosis in HL-60 cells.
DOI： 10.5650/jos.ess24043
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山﨑 有美, 河野 愛未, 松本 朋子, 大島 達也, 山﨑 正夫
日本食品科学工学会誌 71 ( 5 ) 181 - 181 2024年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本食品科学工学会
第71巻3号P.83–91 単位修正p.84右側51行目 （誤）100 mg/mL →（正）100 μg/mLp.85左側16行目 （誤）0.45 mm →（正）0.45 μmp.85左側30行目 （誤）1 mL →（正）1 μLp.85右側10行目 （誤）100 mg/mL →（正）100 μg/mLp.85右側20行目 （誤）2.1 mL →（正）2.1 μLp.85右側25行目 （誤）300 mL →（正）300 μLp.85右側35行目 （誤）0.4 mm →（正）0.4 μmp.86左側12行目 （誤）750 mL →（正）750 μLp.86左側30行目 （誤）188.5 mg/100 g →（正）188.5 μg/100 gp.86左側31行目 （誤）248.2 mg/100 g →（正）248.2 μg/100 gp.86右側7行目 （誤）123.7 mg →（正）123.7 μgp.86右側9行目 （誤）112.2 mg →（正）112.2 μgp.87左側1行目 （誤）149.4 mg →（正）149.4 μgp.87左側3行目 （誤）138.0 mg →（正）138.0 μgp.87左側5行目 （誤）111.7 mg →（正）111.7 μgp.88上 図6・y軸単位 （誤）(μg) →（正）(ng)p.88左側29行目 （誤）1.02 mg →（正）1.02 ngp.88右側2行目 （誤）15.62 mg →（正）15.62 ngp.88右側6行目 （誤）13.38 mg →（正）13.38 ng
DOI： 10.3136/nskkk.71.181
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ヒト腸管上皮細胞モデル (Caco-2) におけるキンカン由来β-クリプトキサンチンの膜透過性に及ぼす麹菌発酵乳飲料の影響 査読あり
山﨑 有美, 河野 愛未, 松本 朋子, 大島 達也, 山﨑 正夫
日本食品科学工学会誌 71 ( 3 ) 83 - 91 2024年3月
担当区分：最終著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本食品科学工学会
キンカンを加工し機能性成分であるβ-クリプトキサンチンの含有量を比較したところ, ペーストにより多くのβ-クリプトキサンチンが含まれ, ペーストと麹菌発酵乳飲料を混合しても, β-クリプトキサンチン含有量は変化しなかった. また, キンカンペースト添加麹菌発酵乳飲料を65 °C 及び 85 °Cで殺菌処理したところ, 65 °C試験区において多くのβ-クリプトキサンチンが残存することが示された. キンカン由来β-クリプトキサンチン－麹菌発酵乳飲料混合物のβ-クリプトキサンチン腸管吸収能を評価した結果, β-クリプトキサンチン単独区と比較し, 麹菌発酵乳飲料混合区の腸管吸収量は約14倍に上昇することが明らかとなった.
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<i>Lactiplantibacillus plantarum</i> 06CC2 upregulates intestinal ZO-1 protein and bile acid metabolism in Balb/c mice fed high-fat diet 査読あり
YAMASAKI Masao, MIYAMOTO Yuko, OGAWA Kenjirou, NISHIYAMA Kazuo, TSEND-AYUSH Chuluunbat, LI Yiran, MATSUSAKI Tatsuya, NAKANO Tomoki, TAKESHITA Masahiko, ARIMA Yuo
Bioscience of Microbiota, Food and Health advpub ( 0 ) 2024年1月
担当区分：最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：BMFH出版会
The effects of <i>Lactiplantibacillus plantarum </i>06CC2 (LP06CC2), which was isolated from a Mongolian dairy product, on lipid metabolism and intestinal tight junction–related proteins in Balb/c mice fed a high-fat diet (HFD) were evaluated. The mice were fed the HFD for eight weeks, and the plasma and hepatic lipid parameters, as well as the intestinal tight junction–related factors, were evaluated. LP06CC2 slightly reduced the adipose tissue mass. Further, it dose-dependently decreased plasma total cholesterol (TC). The HFD tended to increase the plasma level of endotoxin and suppressed intestinal ZO-1 expression, whereas a low LP06CC2 dose increased ZO-1 expression and tended to reduce the plasma lipopolysaccharide level. Furthermore, a low LP06CC2 dose facilitated a moderate accumulation of Lactobacillales, a significant decrease in <i>Clostridium </i>cluste<i>r </i>IV, and an increase in <i>Clostridium </i>cluster<i></i>XVIII. The results obtained from analyzing the bile acids (BAs) in feces and cecum contents exhibited a decreasing trend for secondary and conjugated BAs in the low LP06CC2–dose group. Moreover, a high LP06CC2 dose caused excess accumulation of Lactobacillales and failed to increase intestinal ZO-1 and occludin expression, while the fecal butyrate level increased dose dependently in the LP06CC2-fed mice. Finally, an appropriate LP06CC2 dose protected the intestinal barrier function from the HFD and modulated BA metabolism.
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Role of Extracellular Vesicles in Absorption and Functional Mechanisms of Quercetin 査読あり
Ishisaka A., Sugimoto R., Marumo H., Doi T., Hamada K., Fujimoto M., Fujiwara N., Yamasaki M., Murakami A.
Molecular Nutrition and Food Research 67 ( 21 ) e2300225 2023年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Molecular Nutrition and Food Research
Scope: Quercetin (QUE), a phytochemical found in various plant foods, has been shown to have a variety of physiological activities in vivo, though biological sites where it has activities and the mechanisms of transport have not been fully elucidated. Methods and results: In the present study, intracellular uptake of QUE into HT-29 human colon adenocarcinoma cells is found to result in spontaneous release of extracellular vesicles (EVs), which are subsequently embedded with QUE. In addition, QUE-embedded EVs are detected in serum of QUE-administered Sprague-Dawley rats. Interestingly, the rate of cellular uptake of QUE-encapsulated EVs (EV-QUE) into RAW264.7 macrophages is markedly higher than that of free QUE. Moreover, EV-QUE suppresses lipopolysaccharide (LPS)-induced nitric oxide at a lower concentration than free QUE. Conclusion: The present findings suggest that QUE may be embedded in EVs in the gastrointestinal tract, then become absorbed and enter the bloodstream to exhibit biological activities.
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Ogawa Kenjirou, Karitani Takara, Otsu Wataru, Nishiyama Kazuo, Kunitake Hisato, Goto Yo, Nomiyama Shota, Hara Hideaki, Yamasaki Masao
BPB Reports 6 ( 3 ) 87 - 97 2023年5月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本薬学会
<i>Background</i>: Blue light causes retinal photoreceptor damage via oxidative and endoplasmic reticulum (ER) stress. A previous study showed that blueberry stem extract (BStEx) and its active components have cytoprotective effects against blue-light-induced photoreceptor cell damage by suppressing oxidative stress. This study demonstrated the inhibitory effect of BStEx against blue light-induced ER stress in photoreceptor cells. <i>Methods</i>: The photoreceptor cells treated with BStEx or the antioxidant <i>N</i>-Acetyl-L-cysteine (NAC) as a positive control were used and then exposed to blue light. The cytoprotective effects of BStEx and NAC were evaluated using CCK-8. The ER stress-related protein expression changes over time, and its levels were measured after each exposure time to blue light in photoreceptor cells treated with BStEx or NAC. <i>Results</i>: BStEx and NAC showed protective effects against blue-light-induced photoreceptor morphological abnormalities and cell damage. Although blue light triggered ER stress factors such as BiP, PERK, ATF6, eIF2α, ATF4, and CHOP, which in turn stimulated cell cycle arrest factors p53 and p21 and upregulation of apoptosis-inducing factors caspase-3. However, BStEx suppressed the increase in expression of BiP, ATF4, ATF6, CHOP, p53, p21, and caspase-3, but not mitochondrial apoptotic factors Bax and cytochrome c. Furthermore, the antioxidant NAC showed similar suppressive effects on BStEx. <i>Conclusion</i>: Our findings suggest that blue light-induced ER stress is primarily caused by oxidative stress, and BStEx might suppress ER stress via an antioxidant effect. The antioxidant NAC contributes to the cell proliferative capacity and suppression of apoptosis in photoreceptor cells.
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Ogawa K., Ohno Y., Tagashira A., Urata K., Satoh K., Fujimoto N., Sonoda H., Ikeda M., Matsuzaki T., Nishiyama K., Kunitake H., Goto Y., Yamasaki M.
In Vivo 37 ( 3 ) 1003 - 1015 2023年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：In Vivo
Background/Aim: Tears secreted from the lacrimal gland are essential for preserving the ocular surface. Thus, dysfunction of the lacrimal gland in Sjögren’s syndrome (SS) can lead to dry eye, resulting in a reduced quality of life. We previously reported that blueberry ‘leaf’ water extract prevents lacrimal hyposecretion in male non-obese diabetic (NOD) mice in a SS-like model. In this study, we investigated the effect of blueberry ‘stem’ water extract (BStEx) on lacrimal hyposecretion in NOD mice. Materials and Methods: Male NOD mice were fed 1% BStEx or control (AIN-93G) for 2, 4, or 6 weeks from 4 weeks of age. Pilocarpine-induced tear secretion was measured using a phenol red-impregnated thread. The lacrimal glands were histologically evaluated by HE staining. Inflammatory cytokine levels in the lacrimal glands were measured using ELISA. Immunostaining was performed to examine aquaporin 5 (AQP5) localization. The expression levels of autophagy-related proteins, AQP5, and phosphorylated AMPK were measured using western blotting. Results: After feeding BStEx to mice for 4 or 6 weeks, tear volume was observed to have increased in the BStEx group compared with that in the control group. There were no significant differences in inflammatory cell infiltration, autophagy-related protein expression, or the localization and expression of AQP5 in the lacrimal glands between the two groups. In contrast, AMPK phosphorylation increased in the BStEx group. Conclusion: BStEx prevented lacrimal hyposecretion in the SS-like model of male NOD mice, probably by opening tight junctions via the activation of AMPK in lacrimal acinar cells.
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Toyama Y., Fujita Y., Toshima S., Hirano T., Yamasaki M., Kunitake H.
Plants 12 ( 4 ) 2023年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plants
Polyphenol-rich rabbiteye blueberry (Vaccinium virgatum Aiton) leaves have attracted attention as a food material. In this study, we compared the total polyphenols, total proanthocyanidin content, and antioxidant activity of the leaves of 18 blueberry varieties and investigated the seasonal variation in polyphenols. We also evaluated the anti-cancer cell proliferation properties of the rabbiteye blueberry leaf specific cultivar ‘Kunisato 35 Gou’. Rabbiteye blueberry leaves had significantly higher total polyphenol and total proanthocyanidin values than northern highbush blueberry and southern highbush blueberry leaves. The antioxidant activity of blueberry leaves was highly positively correlated with both the total polyphenol and total proanthocyanidin content. Variations were observed in the total polyphenol and total proanthocyanidin content of rabbiteye blueberry leaves harvested at different points in the growing season; leaves collected in fall to winter contained more epicatechin in addition to proanthocyanidins. In the evaluation of anti-cancer cell proliferation properties against HL-60 promyelocytic leukemia cells, the September-harvested extracts of rabbiteye blueberry ‘Kunisato 35 Gou’ showed strong properties, and the use of an FITC Annexin V apoptosis detection kit with propidium iodide confirmed that this HL-60 cell death occurred via apoptosis. Limiting the harvest time would make rabbiteye blueberry leaves a more functional food ingredient.
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Blueberry stem extract and stem active components prevent blue light-emitting diode light-induced retinal photoreceptor cell damage in vitro 査読あり
Kenjirou Ogawa, Karin Urata, Yosuke Suzuki, Kazuhiro Sugamoto, Yo Goto, Takayuki Nakayama, Kazuo Nishiyama, Hisato Kunitake, Masao Yamasaki
Bioscience, Biotechnology, and Biochemistry 2023年1月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1093/bbb/zbad001
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Ogawa K., Urata K., Maeda S., Ohno Y., Satoh K., Yamada Y., Suzuki Y., Koga Y., Sugamoto K., Kawaguchi M., Kunitake H., Nishiyama K., Goto Y., Nakayama T., Yamasaki M.
In Vivo 37 ( 1 ) 149 - 162 2023年1月
担当区分：最終著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：In Vivo
Background/Aim: This study evaluated the effect of blueberry leaf hot water extract (BLEx) on Sjögren's syndrome (SS)-like lacrimal hyposecretion in male non-obese diabetic (NOD) mice. Materials and Methods: NOD or BALB/c mice were fed 1% BLEx or control (AIN-93G) for 2 weeks from the age of 4 to 6 weeks. Pilocarpine-induced tear volume was measured using a phenol red-impregnated thread. The lacrimal glands were evaluated histologically by H&E staining. The IL-1β and TNF-α levels in the lacrimal gland tissue were measured by ELISA. The mRNA expression levels of secretion-related proteins were measured by real-time PCR. LC3 I/II and arginase 1 expression levels were measured by western blot. Results: After feeding with BLEx, pilocarpine-induced tear secretion in NOD mice was increased. In contrast, the mRNA expression levels of the cholinergic muscarinic M3 receptor, aquaporin 5, and ion channels related to lacrimal secretion were not changed by BLEx administration. In addition, the protein expression of arginase 1, which was recently reported to be involved in tear hyposecretion in NOD mice, was also not improved by BLEx administration. Although infiltration in the lacrimal gland of NOD mice was not decreased, the levels of TNF-α and the autophagy-related protein LC3 were significantly suppressed by BLEx treatment. Conclusion: BLEx treatment may ameliorate lacrimal hyposecretion in NOD mice by delaying the progression of autoimmune disease by suppressing autophagy in lacrimal glands.
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Autophagy in adipogenesis: Molecular mechanisms and regulation by bioactive compounds 査読あり 国際共著
Khan F, Khan H, Khan A, Yamasaki M, Moustaid-Moussa N, Al-Harrasi A, Rahman SM.
Biomed Pharmacother 113715 2022年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Kai H., Okada Y., Goto Y., Nakayama T., Sugamoto K., Ogawa K., Yamasaki M., Morishita K., Matsuno K., Kunitake H.
Plants 11 ( 10 ) 2022年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plants
Although Vaccinium virgatum Aiton leaves and stems inhibit adult T-cell leukemia (ATL) cells, leaves and stems can differ between individual plants and by time and location. In this study, leaf and stem components were profiled in the same individual plant using direct-injection electron ionization-mass spectrometry (DI-EI-MS) metabolomics, with the aims of analyzing the anti-ATL activity, and quantifying proanthocyanidins (PACs). Leaves, stems, and leaf/stem mixtures showed distinct and characteristic spectra. Anti-ATL activity was stronger in stems than leaves, and the PAC content was higher in stems than leaves. These data were subjected to bivariate analysis to identify the factor (m/z) responsible for the inhibitory effect of ATL based on the highest coefficient of determination (R2 ). The results of this DI-EI-MS metabolomics analysis suggest that among PACs contained in V. virgatum stems and leaves, the fragment ion at m/z 149 contributes significantly to anti-ATL activity.
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Yamasaki M., Kiue Y., Fujii K., Sushida M., Yamasaki Y., Sugamoto K., Suzuki Y., Koga Y., Kunitake H., Kai H., Ogawa K., Nishiyama K., Goto Y., Nakayama T.
Plants 10 ( 12 ) 2021年12月
担当区分：筆頭著者, 責任著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plants
Blueberry (Vaccinium virgatum Aiton; Kinisato 35 Gou) leaves have recently attracted in-creasing attention as a useful material for the prevention of lifestyle diseases. Here, we examined the effects of the hot water extract of blueberry leaves (BLEx) on lipogenesis and uric acid production in 3T3-L1 adipocytes. The results showed that BLEx suppressed lipid accumulation and the mRNA expression of differentiation markers in 3T3-L1 adipocytes. A fractionation study showed that the highly polymerized proanthocyanidin-rich fraction was responsible for this effect. Upon maturation to adipocytes, 3T3-L1 cells produced uric acid and tumor necrosis factor-α, and hypoxia stimulated the production of uric acid and xanthine oxidoreductase activity. BLEx suppressed the production of uric acid under these conditions. Although BLEx inhibited the enzymatic activity of xanthine oxidase, this activity was observed in several fractions containing catechin, epicatechin, chlorogenic acid, rutin, and low molecular weight proanthocyanidins. Taken together, these results indicate that BLEx contains various compounds with the ability to suppress lipid accumulation and uric acid production in adipocytes.
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Enhanced water dispersibility and permeability through a Caco-2 cell monolayer of β-cryptoxanthin extracted from kumquats by complexation with casein 査読あり
Ohima T, Takanashi K, Inada A, Yamasaki M, Yamasaki Y, Eto N
Food Chemistry 360 129822 2021年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, however, Their Intracellular Incorporation by Endocytosis Was Not Involved in this Effect on RAW264 Cells. 査読あり
Yamasaki M, Yamasaki Y, Furusho R, Kimura H, Kamei I, Sonoda H, Ikeda M, Oshima T, Ogawa K, Nishiyama K
Molecules 26 ( 9 ) 2763 2021年5月
担当区分：筆頭著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Role of macrophage autophagy in atherosclerosis: modulation by bioactive compounds. 査読あり
Zahid MK, Sufian HB, Choudhury M, Yamasaki M, Al-Harrasi A, Moustaid-Moussa N, Rahman SM
Biochemical Journal 478 1359 - 1375 2021年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1042/BCJ20200894
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地域素材を活用した高機能性食品の開発 -宮崎完熟きんかん「たまたま」を使用した加工特性解析と加工品の開発-
山﨑 有美, 坂元 亜美, 河野 愛未, 大島 達也, 髙橋 幸毅, 篠原 有紀子, 福井 敬一, 吉山 佳世, 近藤 知巳, 山﨑 正夫
宮崎大学地域資源創成学部紀要 ( 4 ) 129 - 142 2021年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：宮崎大学地域資源創成学部