論文 - 山﨑 正夫
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榊原 陽一, 中原 幸太, 森永 浩通, 山崎 正夫, 西山 和夫, 水光 正仁
生物工学会誌 : seibutsu-kogaku kaishi 89 ( 10 ) 593 - 596 2011年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本生物工学会
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Novel screening method for potential skin-whitening compounds by a luciferase reporter assay
Shirasugi I., Sakakibara Y., Yamasaki M., Nishiyama K., Matsui T., Liu M., Suiko M.
Bioscience, Biotechnology and Biochemistry 74 ( 11 ) 2253 - 2258 2010年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Measurement of the melanin content by using B16 melanoma cells is generally applied to find novel skinwhitening agents. However, this measurement method using B16 melanoma cells has such disadvantages, as the time taken, its sensitivity, and troublesomeness. We therefore attempted in the present study to establish a reporter assay system by measuring the tyrosinase promoter activity to use for convenient, high-throughput screening of new melanogenesis inhibitors. We first confirmed the validity of this reporter assay system by using such known skin-whitening agents, as arbutin, sulforaphane, and theaflavin 3,3'-digallate. We then compared the effect of 56 compounds on the tyrosinase promoter activity to test this reporter assay system. Carnosol, and rottlerin strongly inhibited the tyrosinase promoter activity. Moreover, carnosol and rottlerin decreased melanin synthesis and tyrosinase expression in a dose-dependent manner when using B16 melanoma cells. These results indicate this new luciferase reported assay system to be an effective and convenient method for screening potential skin-whitening compounds.
DOI： 10.1271/bbb.100466
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Genistein induced apoptotic cell death in adult t-cell leukemia cells through estrogen receptors
Yamasaki M., Mukai A., Ohba M., Mine Y., Sakakibara Y., Suiko M., Morishita K., Nishiyama K.
Bioscience, Biotechnology and Biochemistry 74 ( 10 ) 2113 - 2115 2010年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Adult T-cell leukemia (ATL) occurs in human T-lymphotropic virus type I-infected individuals and is endemic to the southwestern area of Kyushu in Japan. Here, we found that nM levels of genistein and 17-estradiol had cytotoxic effects on ATL cells and activated caspase-3. The estrogen receptor antagonist ICI182780 negated the cytotoxic effect of genistein. In addition, G protein-coupled estrogen receptor agonist G-1 also had a cytotoxic effect on ATL cells. This is the first report suggesting that estrogen receptors are a molecular target for ATL therapy.
DOI： 10.1271/bbb.100359
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山崎 正夫, 西山 和夫
バイオサイエンスとインダストリー = Bioscience & industry 68 ( 5 ) 332 - 335 2010年9月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：バイオインダストリー協会
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Effects of highly ripened cheeses on HL-60 human leukemia cells: antiproliferative activity and induction of apoptotic DNA damage. 査読あり
Yasuda S, Ohkura N, Suzuki K, Yamasaki M, Nishiyama K, Kobayashi H, Hoshi Y, Kadooka Y, Igoshi K.
Journal of Dairy Science 93 ( 4 ) 1393 - 1400 2010年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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スイス留学体験記
山崎正夫
生物機能研究会誌 2009年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Omi Y., Fujii N., Ozaki A., Nakama A., Sakakibara Y., Suiko M., Nishiyama K.
Bioscience, Biotechnology and Biochemistry 73 ( 10 ) 2217 - 2221 2009年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Cruciferous vegetables and their isothiocyanates are promising foods and agents for cancer prevention. We focus here on the effects of mustard oil (SMO) in a variety of the Japanese radish, Shibori Daikon (Raphanus sativus), on the proliferation of 3Y1 rat fibroblasts and the H-ras-transformed derivative, HR-3Y1-2. SMO (1.6 μ, g/ml) inhibited the proliferation of HR-3Y1-2, but not 3Y1 after 24h after treatment. A cell cycle analysis showed that SMO induced G2/M arrest after 6 h, although this effect was not observed 24 h after the treatment. SMO transiently decreased the cellular reduced glutathione level accompanied with up-regulation of the intracellular reactive oxygen species 2-3 h post-treatment. Glutathione ethyl ester and N- acetyl-L-cysteine prevented the growth inhibitory effect of SMO. This mustard oil extract consisted of 95.6% 4-methylthio-3-butenyl isothiocyanate and 4.4% 4-methylthiobutyl isothiocyanate. SMO selectively inhibited H-ras-transformed 3Y1 cells associated with transient oxidative stress via reduced glutathione (GSH) depletion.
DOI： 10.1271/bbb.90322
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Fucoidan induces apoptosis through activation of caspase-8 on human breast cancer MCF-7 cells
Yamasaki-Miyamoto Y., Yamasaki M., Tachibana H., Yamada K.
Journal of Agricultural and Food Chemistry 57 ( 18 ) 8677 - 8682 2009年9月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Agricultural and Food Chemistry
Fucoidan is an active component of seaweed that has been shown to inhibit proliferation and induce apoptotic cell death in several tumor cells. However, the detailed mechanisms underlying this process have not yet been elucidated. In the present report, we investigated the effect of fucoidan on the induction of apoptosis in human breast cancer MCF-7 cells. Our data demonstrated that fucoidan reduced the viable cell number of MCF-7 cells in a dose- and time-dependent manner. In contrast, fucoidan did not affect the viable cell number of normal human mammary epithelial cells. Results from the apoptosis assay demonstrated that fucoidan induced internucleosomal DNA fragmentation, chromatin condensation, activation of caspase-7, -8, and -9, and cleavage of poly(ADP ribose) polymerase. Furthermore, expression of Bid was decreased, whereas truncated Bid was increased by fucoidan treatment. There was also a decline in cytosolic Bax and a striking increase of cytosolic cytochrome c. Caspase-8-specific inhibitor, z-ITED-fmk, canceled the cytotoxicity of fucoidan, activation of caspase-7, -8, and -9, and a series of changes in Bax, Bid, and cytochrome c. However, caspase-9-specific inhibitor exerted a moderate inhibitory effect on the cytotoxicity of fucoidan. These data indicated that fucoidan could induce apoptotic cell death through a caspase-8-dependent pathway in MCF-7 cells. © 2009 American Chemical Society.
DOI： 10.1021/jf9010406
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Dihydro-alpha-lipoic acid has more potent cytotoxicity than alpha-lipoic acid. 査読あり
M. Yamasaki, A. Kawabe, K. Nishimoto, H. Madhyastha, Y. Sakakibara, M. Suiko, T. Okamoto, T. Suda, K. Uehira, and K. Nishiyama.
In Vitro Cellular Develomental Biology-Animal 45 ( 5-6 ) 275 - 280 2009年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Tachibana H., Yamada A., Ochi Y., Madhyastha H., Nishiyama K., Yamada K.
In Vitro Cellular and Developmental Biology - Animal 44 ( 7 ) 290 - 294 2008年8月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：In Vitro Cellular and Developmental Biology - Animal
Our previous study indicated that oleic acid prevented apoptotic cell death induced by trans10, cis12 (t10, c12)-conjugated linoleic acid in rat hepatoma dRLh-84 cells. The intracellular mechanism of action oleic acid is still unknown. Here, we showed that p38 mitogen-activated protein kinase (MAPK) inhibition using its specific inhibitor SB203580 cancelled the ameliorative effect of oleic acid on the cytotoxicity of t10, c12-conjugated linoleic acid. In addition, SubG1 cell population analysis showed that p38 MAPK played an essential role in the prevention of apoptotic cell death by oleic acid. In fact, p38 phosphorylation level was upregulated in cells treated with oleic acid irrespective of t10, c12-conjugated linoleic acid stimulation. Interestingly, t10, c12-conjugated linoleic acid increased intracellular triglyceride accumulation. However, oleic acid completely inhibited this effect. These observations indicated the involvement of blockade of a p38 MAPK pathway in the ameliorative effect of oleic acid on apoptosis induced by t10, c12-conjugated linoleic acid. © 2008 The Society for In Vitro Biology.
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抗変異原性活性をもつ宮崎県産農産物の探索 査読あり
岩田 喬子、榊原 陽一、赤松 絵奈、酒井 美穂、柚木崎 千鶴子、山崎 正夫、西山 和夫、水光 正仁
宮崎大学農学部研究報告 54 69 - 76 2008年1月
記述言語：日本語 掲載種別：研究論文（大学，研究機関等紀要）
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Induction of endoreduplication by a JNK inhibitor SP600125 in human lung carcinoma A549 cells. 査読あり
Y. Miyamoto-Yamasaki, M. Yamasaki, H. Tachibana, and K. Yamada.
Cell Biology International 31 ( 12 ) 1501 - 1506 2007年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Soy-derived isoflavones inhibit the growth of adult T-cell leukemia cells in vitro and in vivo
YAMASAKI Masao, FUJITA Satoshi, ISHIYAMA Erina, MUKAI Ayako, MADHYASTHA Harishkumar, SAKAKIBARA Yoichi, SUIKO Masahito, HATAKEYAMA Kinta, NEMOTO Takayuki, MORISHITA Kazuhiro, KATAOKA Hiroaki, TSUBOUCHI Hirohito, NISHIYAMA Kazuo
Cancer science 98 ( 11 ) 1740 - 1746 2007年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Reduced expression of perchloric acid-soluble protein after partial hepatectomy in rats
Kanouchi H., Taga M., Okamoto T., Yamasaki M., Oka T., Yamada K., Tone S., Minatogawa Y.
Bioscience, Biotechnology and Biochemistry 70 ( 1 ) 290 - 292 2006年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
We examined the expression of perchloric acid-soluble protein (PSP) during liver regeneration after partial hepatectomy (PH) in rats. Liver regeneration was almost complete at 7-d after PH. Expression of PSP protein and mRNA decreased and then gradually increased during liver regeneration. An immunohistochemical study showed that PSP is distributed in cytosol and nuclei in normal liver, but localization in the nuclei was not be recognized in the regenerated liver.
DOI： 10.1271/bbb.70.290
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Dietary effect of pomegranate seed oil on immune function and lipid metabolism in mice. 査読あり
M. Yamasaki, T. Kitagawa, H. Chujo, N. Koyanagi, H. Maeda, J. Kohno-Murase, J. Imamura, H. Tachibana, and K. Yamada
Nutrition 22 ( 1 ) 54 - 59 2006年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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リポ酸による白血病細胞HL-60のアポトーシス誘導効果 招待あり
山崎正夫、山根理学、西本健太朗、榊原陽一、水光正仁、西山和夫
Food Function 2 ( 1 ) 1 - 5 2006年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Miyamoto Y., Chujo H., Nishiyama K., Tachibana H., Yamada K.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 1735 ( 3 ) 176 - 184 2005年8月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Conjugated linoleic acid (CLA) is a powerful anti-carcinogenic fatty acid. Previously, we showed that 10trans 12cis (10t, 12c) CLA induced apoptotic cell death in rat hepatoma. Here, we demonstrated significant cytotoxic effects of 1 μM 10t, 12c-CLA, but not 9c, 11t-CLA, on dRLh-84 rat hepatoma cells. 9t, 11t and 9c, 11c-CLA also showed low levels of cytotoxic activity. 10t, 12c-CLA activated caspase-3, 9 followed by cytochrome c release from mitochondria into the cytosol. Inhibitors of caspase-3, 9 blocked the cytotoxicity of 10t, 12c-CLA. 10t, 12c-CLA also induced translocation of Bax protein into the mitochondrial membrane and cleavage of Bid protein. Lysosomal destabilization induced by 10t, 12c-CLA was observed by monitoring the re-localization of Acridine Orange and the leakage of β-hexosaminidase from lysosomes. 10t, 12c-CLA directly degraded the isolated lysosomes from the rat liver. Our observations indicate that 10t, 12c-CLA induces mitochondria-related apoptosis accompanied by lysosomal destabilization in rat hepatoma cells. © 2005 Elsevier B.V. All rights reserved.
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Cytotoxity of the trans10, cis12 isomer of conjugated linoleic acid on rat hepatoma and its modulation by other fatty acids and tocopherol and tocotrienol. 査読あり
M. Yamasaki, E. Nishida, S. Nou, H. Tachibana, K. Yamada.
In Vitro Cellular Develomental Biology-Animal 41 ( 7 ) 239 - 244 2005年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Nakaya M., Yamasaki M., Tachibana H., Yamada K.
Immunology Letters 98 ( 2 ) 225 - 231 2005年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Immunology Letters
Estrogens have diverse effects on cell growth, differentiation and homeostatic functions, and have been shown to play an important role in regulating immune system. In this study, we examined the effect of 17β-estradiol (E2) on antibody production by splenocytes isolated from C57BL/6N mice. Our results suggest that the activation of immunoglobulin (Ig) M production by E2 requires direct cell-cell interaction between adherent and non-adherent cells in mouse splenocyte population, and the primary target of E2 is adherent cell population. In addition, we indicated that ER antagonist ICI 182780 suppressed this enhancing effect of E2. Both ERα agonist and ERα agonist enhanced IgM production of mouse splenocytes. ERs are expressed on plasma membrane as well as in nucleus. However, a plasma membrane-associated ER specific ligand has no stimulation effect on IgM production. In conclusion, our results indicate that adherent cells stimulated by E2 up-regulate IgM production of lymphocytes through the direct cell-cell interactions, and the enhancing effect of E2 is arouse through ERα and ERβ on these cells. © 2004 Elsevier B.V. All rights reserved.
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Gamma-linoleic acid(GLA)による肝再生促進効果に関する研究 査読あり
辻田英司, 島田光生、武冨紹信、祇園智信、調憲、田中真二、伊藤心二、山崎正夫、山田耕路、前原喜彦
外科と代謝・栄養 38 ( 3 ) 119 2004年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌）