論文 - 山﨑 正夫
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Toyama Y., Toshima S., Hirano T., Yamasaki M., Kunitake H.
Journal of Berry Research 11 ( 4 ) 689 - 704 2021年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Berry Research
BACKGROUND: In blueberries, the total polyphenol contents, total anthocyanin contents, and antioxidant activities of fruits in blueberries and their relatives are becoming important targets for breeders. Recently, intersectional hybrids between Japanese wild species Shashanbo and highbush blueberry were produced using polyploid breeding for the first time. However, the polyphenol contents, antioxidant activities, and anti-cancer cell proliferation properties of the fruits of these intersectional hybrids have not been studied sufficiently. OBJECTIVE: The objectives of this study were to determine the anthocyanin contents, polyphenol contents, and antioxidant activities at each stage of fruit development in intersectional hybrids, and to examine the effects of the fruit extracts on anti-cancer cell proliferation properties using human promyelocytic leukemia (HL-60) cells. METHODS: Freeze-dried fruit samples of three intersectional hybrids and their parents at five developmental stages were used to evaluate their polyphenol contents, anthocyanin contents, and levels of two types of antioxidant activities (DPPH and ORAC). The anti-cancer cell proliferation properties of each extract were also evaluated according to the survival rate of HL-60 cells. RESULTS: The total polyphenol contents in fruits of the intersectional hybrids gradually decreased as the fruits grew, but the contents increased slightly at the mature stage. Meanwhile, the total anthocyanin contents increased strongly to the mature stage. In addition, the fruit extract of intersectional hybrid JM1 was found to be more effective for inhibiting the growth of HL-60 human leukemia cells in vitro. Total polyphenol content correlated highly with antioxidant activities and anti-cancer cell proliferation properties. CONCLUSIONS: We clarified that the intersectional hybrids had produced have abundant bioactive compounds, high levels of antioxidant activities, and anti-cancer cell proliferation properties. The results proved the effectiveness of the use of Japanese wild Vaccinium species for the strategic breeding of blueberries with high levels of bioactive compounds.
DOI： 10.3233/JBR-210713
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Yamasaki M., Minesaki M., Iwakiri A., Miyamoto Y., Ogawa K., Nishiyama K., Tsend-Ayush C., Oyunsuren T., Li Y., Nakano T., Takeshita M., Arima Y.
Food Science and Nutrition 8 ( 11 ) 6164 - 6173 2020年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Food Science and Nutrition
© 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC Previous study suggested that dietary intake of Lactobacillus plantarum 06CC2 (LP06CC2) isolated from Mongolian dairy products showed various health beneficial effects. Here, the effect of LP06CC2 on the cholesterol metabolism in mice fed a cholesterol-loaded diet was evaluated. Cholesterol and LP06CC2 were incorporated into the AIN93G-based diet to evaluate the effect on cholesterol metabolism in Balb/c mice. Serum and liver cholesterol levels were significantly increased in mice fed a cholesterol-loaded diet whereas the LP06CC2 ingestion suppressed the increase of liver cholesterol. LP06CC2 suppressed the increase of the hepatic damage indices. The increase of the cecal content and fecal butyrate were observed in mice fed LP06CC2. The analysis of bile acids clearly showed that LP06CC2 increased their deconjugation indicating the decrease of bile acid absorption. The protein expression of hepatic Cyp7A1 was also suppressed by LP06CC2 in mice fed cholesterol. Finally, in vitro studies showed that LP06CC2 had the most potent ability to deconjugate bile acids using glycocholate among the tested probiotic lactic acid bacteria isolated from Mongolian dairy products. Taken together, LP06CC2 is a promising microorganism for the reduction of the cholesterol pool via modulation of bile acid deconjugation.
DOI： 10.1002/fsn3.1909
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奄美島豚の肉質評価に関する研究 査読あり
叶内宏明、 山﨑正夫、鞍掛文、木村郁夫、大塚彰
鹿児島大学紀要 70 1 - 6 2020年3月
記述言語：日本語 掲載種別：研究論文（大学，研究機関等紀要）
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Shoji K., Yamasaki M., Kunitake H.
Journal of Oleo Science 69 ( 2 ) 143 - 151 2020年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oleo Science
©2020 by Japan Oil Chemists’ Society. Prevention of postprandial hypertriglyceridemia is an important consideration for reducing the risk of developing cardiovascular disease. While blueberry fruits have been reported to ameliorate lipid metabolism in humans, there are only few research reports on the effects of blueberry leaves (BL). Here, we investigated the efficacy of BL on postprandial hyperlipidemia in subjects with high fasting triacylglycerol (TG) concentrations. Randomized, double-blind, cross-over design study was conducted. The subjects consumed a BL containing beverage or a placebo beverage before a fat-enriched test meal. Blood samples were collected prior to and 1, 2, 3, 4, and 5 hours after consuming the test beverage. The postprandial serum TG and remnant-like particle cholesterol (RLP-C) concentrations were significantly lower in the BL beverage compared with those in the placebo beverage. Additionally, BL was more effective in subjects with high fasting ghrelin with gastric emptying function. In current study, fasting ghrelin correlated with the increase in postprandial serum TG, suggesting that BL ameliorates hypertriglyceridemia through delayed gastric emptying. In conclusion, this pilot study suggests that BL may be useful as an early dietary therapy for treating postprandial hyperlipidemia.
DOI： 10.5650/jos.ess19201
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エタノール・アセトアルデヒド併用処理によるアルコール性肝細胞毒性の誘導効果と酸化ストレスとの関係
山﨑 楓, 西山 和夫, 山﨑 正夫
日本醸造協会誌 115 ( 7 ) 419 - 428 2020年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益財団法人 日本醸造協会
本研究は，エタノールとアセトアルデヒドの併用処理による肝細胞への影響とそのメカニズムを解明することが目的である。ラット正常肝細胞において，エタノールとアセトアルデヒドを併用処理すると，生細胞数の有意な減少，G0/G1期細胞の割合の有意な増加，G2/M期細胞の割合の有意な減少が確認された。さらに，細胞内ROS量の有意な増加も確認された。このことから，エタノールとアセトアルデヒドの共存によって酸化ストレス，細胞周期の遅延を介して，肝細胞毒性が誘導されることが示唆された。しかし，細胞内ROS量の増加においては，エタノール単独処理でも同レベルの変化が確認されたことから，エタノールとアセトアルデヒドの共存下における肝細胞毒性の増強には，細胞内ROS量は関与していないが，アセトアルデヒドが細胞内ROS量以外の部分で補助的に作用している可能性がある。また，エタノール・アセトアルデヒド併用処理による細胞内ROS量の増加に対して，CYP2E1の阻害剤を処理すると，有意に改善させたが，NADPHオキシダーゼの阻害剤を処理すると変化しなかったことから，エタノール・アセトアルデヒド併用処理による酸化ストレスの誘導は，CYP2E1の活性が関与していることが示唆された。以上のことから，エタノールとアセトアルデヒドの併用処理は，酸化ストレスの誘導と細胞周期の遅延を介して，アルコール性肝細胞毒性を誘導することが示唆された。そして，エタノール・アセトアルデヒド併用処理による酸化ストレスの誘導は，CYP2E1によるものであることが分かった。
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Kosakai T., Kato H., Sho C., Kawano K., Iwai K., Takase Y., Ogawa K., Nishiyama K., Yamasaki M.
PeerJ 2019 ( 9 ) 2019年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：PeerJ
Copyright © 2019 Kosakai et al. It has been reported that fermented products (FPs) prepared from sweet potatoshochu distillery by-product suppressed weight gain and decreased serum cholesterol levels in mice under normal dietary conditions. Furthermore, from the information gained from the above data regarding health benefits of the FPs, the aim of this study was evaluating the effects of dietary FPs on lipid accumulation and gut microbiota in mice with or without cholesterol-load in the diet. C57BL/6N mice were fed normal (CO) diet, CO with 10% FPs (CO + FPs) diet, cholesterol loaded (HC) diet, or HC with 10% FPs (HC + FPs) diet for 8 weeks. The mice were then euthanized, and blood samples, tissue samples, and feces were collected. The adipose tissue weight and liver triglyceride levels in the HC + FPs diet groups were significantly reduced compared to that in the HC diet groups. However, FPs significantly increased the serum non-high-density lipoprotein cholesterol (HDL-C) levels, the ratio of non-HDL-C to HDL-C and hepatic total cholesterol levels in mice fed cholesterolloaded diet compared with that of the HC diet group. Since dietary FPs significantly decreased the protein expression levels of cholesterol 7 alpha-hydroxylase 1 in the HC + FPs diet groups, the cholesterol accumulation in FPs group may be explained by insufficient catabolism from cholesterol to bile acid. In addition, the dietary FPs tended to increase Clostridium cluster IV and XIVa, which are butyrate-producing bacteria. Related to the result, n-butyrate was significantly increased in the CO + FPs and the HC + FPs diet groups compared to their respective control groups. These findings suggested that dietary FPs modulated the lipid pool and gut microbiota.
DOI： 10.7717/peerj.7671
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Inada A., Oue T., Yamashita S., Yamasaki M., Oshima T., Matsuyama H.
European Journal of Pharmaceutical Sciences 136 2019年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：European Journal of Pharmaceutical Sciences
© 2019 Elsevier B.V. The water-solubility of coenzyme Q10 (CoQ10) is extremely low because of its hydrophobicity, which results in low bioavailability. In this study, peptide mixtures derived from different proteins (casein, albumin, gelatin, lysozyme, zein and hemoglobin) were prepared and used as dispersants ofCoQ10. Most peptide mixtures, except for that derived from lysozyme, enhanced the water-dispersibility of CoQ10 by forming complexes with CoQ10. In particular, the apparent solubility of CoQ10complexed with the casein hydrolysate (Pepcas) was the highest. Pepcas was fractionated by ammonium sulfate precipitation and ultrafiltration to find the most effective peptides for enhancing water-dispersibility of CoQ10. The peptide fraction that had a relatively hydrophobic nature and peptides of higher-than-average molecular weights (PepcasA) was found to be the most effective. Results from differential thermal analysis and powder X-ray diffraction revealed that the complex between CoQ10 and PepcasA in the solid state was amorphous. In solution, the complex is a hydrocolloid with particle sizes of 154–279 nm.
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Novel fermented products made from sweet potato-shochu distillery by-products reduces body fat and serum cholesterol in mice. 査読あり
T. Kosakai, R. Nobetsu, C. Sho, K. Kawano, K. Iwai, Y. Takase, K. Nishiyama, M. Yamasaki
Journal of The Brewing Society of Japan 114 ( 5 ) 294 - 301 2019年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Chronic intake of high-dose of blueberry leaf extract does not augment the harmful effects of ethanol in rats. 査読あり
K. Yamasaki, K. Sugamoto, T. Arakawa, K. Nishiyama, M. Yamasaki
PeerJ 2019年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Blueberry Leaf Polyphenols Prevent Body Fat Accumulation in Mice Fed High-fat, High-sucrose Diet
Fujii K., Ota Y., Nishiyama K., Kunitake H., Yamasaki Y., Tari H., Araki K., Arakawa T., Yamasaki M.
Journal of oleo science 68 ( 5 ) 471 - 479 2019年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of oleo science
Blueberry leaf is currently a popular dietary supplement. Effects of dietary blueberry leaf and its active components on body fat accumulation were examined. C57BL/6J mice were fed high-fat, high-sucrose diet with or without 3% blueberry leaf extract (BLEx) or 3% concentrated-polyphenolic BLEx (CP BLEx) for 8 weeks. Compared to mice fed a high-fat, high-sucrose diet without blueberry leaf, BLEx and CP BLEx significantly reduced body weight and adipose tissue weight gain. Adipocytes were also smaller and and liver lipid accumulatioin was significantly inhibited in mice fed either BLEx or CP BLEx. These effects tended to be more pronounced in mice fed CP BLEx compared to in mice fed BLEx. Together, results suggest that blueberry leaf inhibits body fat accumulation typically observed in mice fed a high-fat, high-sucrose diet, and that inhibition is attributable to polyphenolic components in leaf extracts.
DOI： 10.5650/jos.ess18226
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Yamasaki M., Hamada K., Fujii K., Nishiyama K., Yamasaki Y., Tari H., Araki K., Arakawa T.
Biochemistry and Biophysics Reports 14 182 - 187 2018年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Biochemistry and Biophysics Reports
© 2018 The Authors Blueberry (Vaccinium ashei) leaf has recently attracted attention as an anti-obesity food component. In this study, we examined the effects of blueberry leaf extract (BLEx) on insulin signaling in C2C12 differentiated myoblasts. The results showed that BLEx promotes the intracellular uptake of 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]−2-deoxy-D-glucose (2-NBDG) and phosphorylation of Akt under insulin stimulation. In addition, pretreatment with BLEx ameliorated TNF-α-nduced insulin resistance with regard to 2-NBDG uptake and Akt phosphorylation. Moreover, BLEx prevented the TNF-α−induced activation of JNK and NF-kB pathways and phosphorylation of IRS-1 at serine residue. BLEx failed to induce phosphorylation of AMPK as well as did not prevent the restoration of 2-NBDG uptake under TNF-α–induced insulin resistance. Overall, skeletal muscle is a putative target for the anti-diabetic effect of BLEx by amelioration of insulin resistance.
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Dietary borage oil promotes gamma-linoleic acid accumulation in dextran sulfate sodium-treated mice but does not manipulate the severity of colitis 査読あり
R. Fujioka, K. Nishiyama, M. Yamasaki
Journal of Food and Nutrition Research 5 ( 3 ) 151 - 155 2017年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Nakanishi T., Tokunaga Y., Yamasaki M., Erickson L., Kawahara S.
Animal Science Journal 87 ( 12 ) 1554 - 1561 2016年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Animal Science Journal
© 2016 Japanese Society of Animal Science Conjugated linoleic acid (CLA) is one of the constituents of animal products with possible health benefits such as anti-carcinogenic and anti-obesity effects. In this study, we investigated the immunomodulatory effects of CLA using a mouse model of allergic dermatitis. Mice were orally administered either a CLA mixture containing equal amounts of 9c, 11 t-CLA and 10 t, 12c-CLA, or high linoleic acid safflower oil, and allergic dermatitis was induced on the ear by repeated topical applications of oxazolone. Oral administration of the CLA mixture but not the high linoleic safflower oil attenuated the symptoms of allergic dermatitis in both ear weights and clinical scores. This effect was associated with decreased levels of ear interleukin-4 (IL-4) and plasma immunoglobulin E. The immunomodulatory effects of the CLA isomers were compared by an in vitro cytokine production assay. The results showed that 9c, 11 t-CLA, the most predominant isomer in animal products, significantly inhibited IL-4 and interferon-γ production from mouse splenocytes with similar potency to 10 t, 12c-CLA. These findings suggest that CLA, a constituent of animal products, has a potentially beneficial effect for amelioration of allergic dermatitis.
DOI： 10.1111/asj.12603
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共役脂肪酸の多様な機能性とその利用 臨床利用への足掛かり 招待あり 査読あり
木下和昭、野見山将太、山崎正夫
日本農芸化学会誌「化学と生物」 2016年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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A single dose of blueberry leaf extract suppresses serum ethanol levels after oral ethanol administration in rats. 査読あり
Yamasaki K, Nagatomo H, Kawamura Y, Sugamoto K, Kai T, Kamenaga K, Takeshita M, Kikuchi Y, Matsuura Y, Yukizaki C, Nishiyama K, Yamasaki M
European Journal of Scientific Research 2016年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Preparation of conjugated linoleic acid nanoemulsions and their biodistribution. 査読あり
K. Kishita, K. Ibaraki, S. Itakura, Y. Yamasaki, N. Nishikata, K. Yamamoto, M. Shimizu, K. Nishiyama, M. Yamasaki
Journal of Oleo Science 65 ( 11 ) 949 - 954 2016年
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Blueberry (Vaccinium virgatum Aiton) leaf extract ameliorates insulin resistance in mice fed a high-fat, high-sucrose diet. 査読あり
Yamasaki M., Matsuyama Y., Hayasegawa R., Hamada K., Nishiyama K., Kai T., Kamenaga K., Arakawa T., Tari H., Shimizu Y., Matsuura Y., Yukizaki C
Food Science and Technology Research 21 ( 6 ) 827 - 833 2015年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Nano-emulsions of conjugated linoleic acid suppresses inflammatory response in macrophage but not in experimental inflammatory bowel disease condition. 査読あり
M. Yamasaki, H. Kanazaki, R. Fujioka, K. Kishita, K. Nishiyama, K. Yamamoto, S. Hamayama, M. Shimizu
European Journal of. Scientific Research 2015年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Imamura K., Asai M., Sugamoto K., Matsumoto T., Yamasaki Y., Kamei I., Hattori T., Kishimoto M., Niisaka S., Kubo M., Nishiyama K., Yamasaki M.
Bioscience, Biotechnology and Biochemistry 79 ( 6 ) 1021 - 1025 2015年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
© 2015 Japan Society for Bioscience, Biotechnology, and Agrochemistry. In this study, we focused on the anti-inflammatory effect of cordycepin, 3′-deoxyadenosine. Cordycepin potently suppressed nitric oxide production in lipo-polysaccharide-stimulated RAW 264.7 cells in an adenosine receptor-independent manner. In addition, inhibitors for adenosine kinase and nucleoside transporter abrogated the action of cordycepin. Thus, we considered that intracellular metabolism cordycepin is important for the anti-inflammatory effect of cordycepin.
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Yamasaki M., Nishimura M., Sakakibara Y., Suiko M., Morishita K., Nishiyama K.
Food and Function 5 ( 11 ) 2842 - 2849 2014年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Food and Function
© 2014 the Partner Organisations. Here, we examined the effect of tocotrienols (T3) on the growth of adult T-cell leukemia (ATL) cells. All three forms (β-, γ-, and δ-T3) inhibited cell proliferation in a dose-dependent manner; δ-T3 showed the strongest growth-inhibitory effect. δ-T3 increased the G1, G2/M, and subG1 populations and induced internucleosomal DNA fragmentation. δ-T3 treatment also increased the levels of cleaved caspase-3, -6, -7, -9, and poly-ADP ribose polymerase (PARP), and this was accompanied by downregulation of Bcl-2, Bcl-xL, and XIAP. Moreover, δ-T3 decreased nuclear p65 NF-κB levels, indicating downregulation of NF-κB activity. This cytotoxic effect of δ-T3 was abrogated by squalene (SQL) but not mevalonate (MVL), farnesyl diphosphate (FPP), geranylgeranyl diphosphate (GGPP), or cholesterol (CL). δ-T3 decreased intracellular SQL levels, and inhibition of de novo cholesterol synthesis did not affect the action of SQL. Furthermore, δ-T3 significantly decreased farnesyl-diphosphate farnesyltransferase 1 (FDFT1) expression. Taken together, it is evident that δ-T3, due to its ability to potently induce apoptosis via the depletion of intracellular SQL, shows the potential to be considered a therapeutic agent in patients with ATL.
DOI： 10.1039/c4fo00635f