論文 - 山﨑 正夫
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The importance of 1,2-dithiolane structure in α-lipolic acid for the downregulation of cell surface β1-integrin expression of human bladder cancer cells. 査読あり
M. Yamasaki, S. Soda, Y. Sakakibara, M. Suiko, K. Nishiyama
Bioscience Biotechnology Biochemistry 78 ( 11 ) 1939 - 1942 2014年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Ishida Y., Yamasaki M., Yukizaki C., Nishiyama K., Tsubouchi H., Okayama A., Kataoka H.
Human Cell 27 ( 2 ) 68 - 77 2014年4月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Human Cell
Adult T-cell leukemia/lymphoma (ATL) is a fatal malignancy caused by infection with human T-lymphotropic virus type-1 and there is no accepted curative therapy for ATL. We searched for biological active substances for the prevention and treatment of ATL from several species of herbs. The ATL cell growth-inhibitory activity and apoptosis assay showed that carnosol, which is an ingredient contained in rosemary (Rosmarinus officinalis), induced apoptosis in ATL cells. Next, to investigate the apoptosis-inducing mechanism of carnosol, we applied proteomic analysis using fluorescent two-dimensional differential gel electrophoresis and mass spectrometry. The proteomic analysis showed that the expression of reductases, enzymes in glycolytic pathway, and enzymes in pentose phosphate pathway was increased in carnosol-treated cells, compared with untreated cells. These results suggested that carnosol affected the redox status in the cells. Further, the quantitative analysis of glutathione, which plays the central role for the maintenance of intracellular redox status, indicated that carnosol caused the decrease of glutathione in the cells. Further, N-acetyl-l-cystein, which is precursor of glutathione, canceled the efficiency of carnosol. From these results, it was suggested that the apoptosis-inducing activity of carnosol in ATL cells was caused by the depletion of glutathione. © 2013 Japan Human Cell Society and Springer Japan.
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Identification of a novel flavonoid glycoside sulfotransferase in Arabidopsis thaliana. 査読あり
Hashiguchi T, Sakakibara Y, Shimohira T, Kurogi K, Yamasaki M, Nishiyama K, Akashi R, Liu MC, Suiko M.
Journal of Biochemistry 155 ( 2 ) 91 - 97 2014年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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α-Lipoic acid suppresses migration and invasion via downregulation of cell surface β1-integrin expression in bladder cancer cells. 査読あり
Yamasaki M, Iwase M, Kawano K, Sakakibara Y, Suiko M, Ikeda M, Nishiyama K.
Journal of Clinical Biochemistry and Nutrition 54 ( 1 ) 18 - 25 2014年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Induction of apoptotic cell death in HL-60 cells by jacaranda seed oil derived fatty acids
Yamasaki M., Motonaga C., Yokoyama M., Ikezaki A., Kakihara T., Hayasegawa R., Yamasaki K., Sakono M., Sakakibara Y., Suiko M., Nishiyama K.
Journal of Oleo Science 62 ( 11 ) 925 - 932 2013年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oleo Science
Various fatty acids are attracting considerable interest for their anticancer effects. Among them, fatty acids containing conjugated double bonds show one of the most potent cytotoxic effects on cancer cells. Here, we focused on the cancer cell killing activity of jacaranda seed oil. The seed oil of jacaranda harvested from Miyazaki in Japan contained 30.9% cis-8, trans-10, cis-12 octadecatrienoic acid, called jacaric acid (JA). Fatty acid prepared from this oil (JFA) and JA strongly induced cell death in human leukemia HL-60 cells. On the other hand, linoleic acid and trans-10, cis-12 conjugated linoleic acid ( < 10 μM) did not affect cell proliferation and viability. An increase in the sub-G 1 population and internucleosomal fragmentation of DNA was observed in JA- and JFA-treated cells, indicating induction of apoptotic cell death. Finally, the cytotoxic effects of JA and JFA were completely abolished by α-tocopherol. Taken together, these data suggest that jacaranda seed oil has potent apoptotic activity in HL-60 cells through induction of oxidative stress. © 2013 by Japan Oil Chemists' Society.
DOI： 10.5650/jos.62.925
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山崎 正夫
日本栄養・食糧学会誌 : Nippon eiy◆U014D◆ shokury◆U014D◆ gakkaishi = Journal of Japanese Society of Nutrition and Food Science 66 ( 5 ) 241 - 247 2013年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本栄養・食糧学会
共役リノール酸を始めとする共役脂肪酸は, 天然界においては比較的まれな脂肪酸であるが, その極めて強い生理機能が細胞, 動物レベルで示されてきた。このような機能性は私たちの健康に大いに貢献できる可能性を秘めており, 共役脂肪酸は機能性食品素材の候補として期待される。著者らは共役脂肪酸の機能性食品への応用を目指し, その体内動態を解析するため, ガスクロマトグラフィーによる分析法の確立を目的とした前処理方法を確立した。さらに, 細胞試験および動物試験により機能性の探索を進め, 共役脂肪酸は脾臓や腸間膜リンパ節リンパ球の抗体産生増強効果や, アポトーシス誘導による肝ガン細胞の致死活性があることを明らかとした。これらの作用は共役リノール酸の異性体により活性が大きく異なり, 共存するモノ不飽和脂肪酸によって機能性が影響を受けることを示した。本総説では共役リノール酸を中心とするこれらの機能性に関する知見を紹介したい。
DOI： 10.4327/jsnfs.66.241
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Inhibitionof cell motility by fucoidan derived from Cladosiphon okamuranus
Yamasaki Y., Ando Y., Yamasaki M., Tachibana H., Yamada K.
Nippon Shokuhin Kagaku Kogaku Kaishi 60 ( 7 ) 357 - 361 2013年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nippon Shokuhin Kagaku Kogaku Kaishi
Fucoidanis anactive component of seaweed that has beenshownto exhibit a number of biological regulation effects, such as inhibition of proliferation and induction of apoptosis, invarious tumor cells. Inthis study, we demonstrated that fucoidanisolated from Cladosiphon okamuranus significantly inhibited cell motility in several cancer cells in a dose-dependent manner. Fucoidan induced the disruption of stress fibers at concentrations greater than 10 μg/mL. However, fucoidanwas not shownto decrease the phosphorylationof the myosinII regulatory light chain(MRLC) at Thr18/Ser19 inB16 cells. These data suggested that the inhibition of cell motility by treatment with fucoidan was induced via a pathway other than MRLC.
DOI： 10.3136/nskkk.60.357
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Genistein induces apoptotic cell death associated with inhibition of the NF-kB pathway in adult T-cell leukemia cells 査読あり
M. Yamasaki, Y. Mine, M. Nishinura, S. Fujita, Y. Sakakibara, M. Suiko, K.Morishita, K. Nishiyama.
Cell Biology international 37 ( 7 ) 742 - 747 2013年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Adipocyte response to conjugated linoleic acid (Review) 査読あり
M. Yamasaki, T. Yanagita
Obesity Research & Clinical Practice 7 e235 - e242 2013年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Jacaric acid is rapidly metabolized to conjugated linoleic acid in rats
Kijima R., Honma T., Ito J., Yamasaki M., Ikezaki A., Motonaga C., Nishiyama K., Tsuduki T.
Journal of Oleo Science 62 ( 5 ) 305 - 312 2013年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oleo Science
We have shown previously that jacaric acid (JA; 8c,10t,12c-18:3), which has a conjugated triene system, has a strong anti-tumor effect. However, the characteristics of absorption and metabolism of JA have yet to be determined in vivo, and the details of absorption and metabolism of JA in the small intestine are particularly unclear. This information is required for effective use of JA in humans. Therefore, in this study we examined absorption and metabolism of JA using cannulation of the thoracic duct in rats. Emulsions of two test oils, jacaranda seed oil and tung oil, which contain JA and α-eleostearic acid (α-ESA; 9c,11t,13t-18:3), respectively, were administered to rats and lymph from the thoracic duct was collected over 24 h. We examined the rate of absorption of JA and possible conversion to a conjugated linoleic acid (CLA)containing a conjugated diene system. The positional isomerism of the CLA produced by JA metabolism was determined using gas chromatography-electron impact/mass spectrometry. The rate of absorption and percentage conversion of JA were compared with those of α-ESA. We found that JA is rapidly absorbed and converted to a CLA in rats and that the percentage conversion of JA was lower than that of α-ESA. This is the first report on the absorption and metabolism of JA and this information may be important for application of JA as a functional food. ©2013 by Japan Oil Chemists' Society.
DOI： 10.5650/jos.62.305
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Tsuda H., Kunitake H., Kawasaki-Takaki R., Nishiyama K., Yamasaki M., Komatsu H., Yukizaki C.
Plants 2 ( 1 ) 57 - 71 2013年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plants
© 2013 by the authors; licensee MDPI, Basel, Switzerland. Antioxidants are abundant in blueberries, and while there are many studies concerning the bioactive compound of fruit, it is only recently that the wild Vaccinium species has attracted attention for their diverse and abundant chemical components. The aim of this study was to investigate the bioactive compounds of blueberry cultivars and wild species found in Japan. Among the five extracts of the Vaccinium species, Natsuhaze (Vaccinium oldhamii Miq.) was found to be the most effective at inhibiting the growth of HL-60 human leukemia cells in vitro. Although all ethanol extracts showed a growth inhibitory effect on HL-60 cells, the degree of the effects differed among the species. The extract of Natsuhaze induced apoptotic bodies and nucleosomal DNA fragmentation in the HL-60 cells. Of the extracts tested, that of Natsuhaze contained the largest amount of total polyphenols and showed the greatest antioxidant activity, but the anthocyanin content of Natsuhaze was similar to that of rabbiteye blueberry (V. virgatum Ait.). The results showed that total polyphenols contributed to the high antioxidant activity and growth inhibitory effect on HL-60 human leukemia cells of Natsuhaze extract.
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Yamasaki M., Mine Y., Nishimura M., Fujita S., Sakakibara Y., Suiko M., Morishita K., Nishiyama K.
Cell Biology International 37 ( 7 ) 742 - 747 2013年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cell Biology International
We have shown that genistein inhibits the growth of adult T-cell leukemia (ATL) cells in vitro and in vivo, and this leads to pronounced G2/M arrest. This report shows that genistein induces apoptotic death in ATL cells. Although the pan-caspase inhibitor, Z-VAD-fmk, did not inhibit genistein-induced apoptosis, release of apoptosis-inducing factor (AIF) into the cytosol occurred. Poly-ADP ribose polymerase inhibition also abrogated genistein-induced apoptosis. Genistein decreased nuclear p65 translocation and IkBα phosphorylation, and downregulated the anti-apoptotic proteins, XIAP, cIAP and survivin, NF-kBresponsive gene products. Thus, genistein is a promising agent for ATL that induces caspase-independent apoptosis through inhibition of the NF-kB pathway. © 2013 International Federation for Cell Biology.
DOI： 10.1002/cbin.10101
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Shinohara N., Ito J., Tsuduki T., Honma T., Kijima R., Sugawara S., Arai T., Yamasaki M., Ikezaki A., Yokoyama M., Nishiyama K., Nakagawa K., Miyazawa T., Ikeda I.
Journal of Oleo Science 61 ( 8 ) 433 - 441 2012年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Oleo Science
Conjugated fatty acid is a collective term used for fatty acids with conjugated double bond systems. Seed oils from certain plants include conjugated linolenic acids, which have a conjugated triene system and are geometrical and positional isomers of α-linolenic acid. One of these isomers, jacaric acid (JA, 8c, 10t, 12c-18:3), has not been examined widely. Therefore, we investigated the absorption and metabolism of JA in normal animals (ICR mice). An oral dose of JA of 5 mg/day for 1 week had no effects on body weight, food intake and tissue weight of mice. JA was detected in the serum, kidney, liver, lung and epididymal white adipose tissue. Analysis of the fatty acid composition in liver and white adipose tissue showed a tendency to increase levels of saturated fatty acids (SFAs) such as palmitic acid (16:0) and stearic acid (18:0) and to decrease levels of monounsaturated fatty acids (MUFAs) such as palmitoleic acid (16:1) and oleic acid (18:1). Thus, JA treatment decreased the desaturation index (16:1/16:0, 18:1/18:0) in liver and white adipose tissue. This index is used as an indicator of the activity of stearoyl coenzyme A desaturase (SCD), an endoplasmic reticulum enzyme that catalyzes the biosynthesis of MUFAs from SFAs. The change in this index indicates that JA inhibited SCD activity in ICR mice, and further experiments showed that JA also decreased the expression level of SCD-1 mRNA. Inhibition of SCD activity may have anti-obesity and anti-diabetes effects, and therefore the findings in this study suggest that JA may be effective for preventing obesity and diabetes. ©2012 by Japan Oil Chemists' Society.
DOI： 10.5650/jos.61.433
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Conjugated linoleic acids inhibit hypoxia inducible factor-1alpha stabilization under hypoxic condition in human hepatocellular carcinoma cells. 査読あり
M. Yamasaki, T Nagatomo, T Matsuyama, Y Ikeho, E Kato, K Nishiyama, Y. Sakakibara, M Suiko, K Nishiyama
Journal of oleo science 61 ( 9 ) 491 - 496 2012年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Jacaric acid, a linolenic acid isomer with a conjugated triene system, has a strong antitumor effect in vitro and in vivo. 査読あり
N Shinohara, T Tsuduki, J Ito, T Honma, R Kijima, S Sugawara, T Arai, M Yamasaki, A Ikezaki, M Yokoyama, K Nishiyama, K Nakagawa, T Miyazawa, I Ikeda.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 1821 ( 7 ) 980 - 988 2012年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Important role of β 1-integrin in fucoidan-induced apoptosis via caspase-8 activation
Yamasaki Y., Yamasaki M., Tachibana H., Yamada K.
Bioscience, Biotechnology and Biochemistry 76 ( 6 ) 1163 - 1168 2012年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Fucoidan induces apoptosis by activating caspase-8 in human MCF-7 breast cancer cells, but the detailed mechanism for this is not understood. We demonstrate here that fucoidan interacted with the cell surface, and silencing the β 1 -integrin gene expression inhibited fucoidan-induced apoptosis accompanied by caspase-8 activation. Fucoidan induced formation of the β 1 -integrin-caspase-8 complex. These data indicate that β 1 -integrin is an important factor for the cell-surface binding of fucoidan and plays an important role in fucoidan-induced apoptosis. Fucoidan also induced recruitment of caspase-8 to the β 1 -integrin intracellular domain, cleaved it into the activated protein by direct combination with β 1 -integrin, and induced apoptosis via the caspase cascade in MCF-7 cells.
DOI： 10.1271/bbb.111001
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Yamasaki M., Iwase M., Kawano K., Sakakibara Y., Suiko M., Nishiyama K.
Bioscience, Biotechnology and Biochemistry 76 ( 6 ) 1177 - 1181 2012年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
We determined the effects of apocynin, a representative inhibitor of NADPH oxidase, on the proliferative and adhesive properties of 3Y1 rat fibroblasts and the 3Y1 v-H-ras-transformed derivative, HR-3Y1-2. Apocynin inhibited the proliferation of HR-3Y1-2 but not 3Y1 cells at 10μM and 100μM. Apocynin also decreased the intracellular reactive oxygen species (ROS) level in HR-3Y1-2 but not 3Y1 cells. We also evaluated the effects of apocynin on cell adhesion to fibronectin and found decreased adhesion of HR-3Y1-2 cells to fibronectincoated plates. Our results indicate that apocynin selectively down-regulated β1-integrin cell surface expression on the HR-3Y1-2 cells. It also inhibited the migration and invasion of these cells. These data suggest that reducing the production of NADPH oxidasemediated ROS could be an effective means for ameliorating the abnormal growth, adhesion and motility of v-H-ras-transformed cells.
DOI： 10.1271/bbb.120061
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Effect of highly lipolyzed goat cheese on HL-60 human leukemia cells: antiproliferative activity and induction of apoptotic DNA damage. 査読あり
asuda, S., Kuwata, H., Kawamoto, K., Shirakawa, J., Hoshi, J., Yamasaki M., Nishiyama K., Tachibana,H., Yamada, K., Kobayashi, H., Igoshi, K.
Journal of Dairy Science 95 ( 5 ) 2248 - 2260 2012年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Alpha Lipoic Acid Selectively Inhibits Proliferation and Adhesion to Fibronectin of v-H-ras-transformed 3Y1 Cells. 査読あり
M. Yamasaki, M. Iwase, K. Kawano, Sakakibara, M. Suiko, and K. Nishiyama.
Journal of Clinical Biochemistry and Nutrition 50 ( 3 ) 234 - 240 2012年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Enzymatic sulfation of tocopherols and tocopherol metabolites by human cytosolic sulfotransferases
Hashiguchi T., Kurogi K., Sakakibara Y., Yamasaki M., Nishiyama K., Yasuda S., Liu M., Suiko M.
Bioscience, Biotechnology and Biochemistry 75 ( 10 ) 1951 - 1956 2011年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Tocopherols are essential micronutrients for mammals widely known as potent lipid-soluble antioxidants that are present in cell membranes. Recent studies have demonstrated that most of the carboxychromanol (CEHC), a tocopherol metabolite, in the plasma exists primarily in sulfate- and glucuronide- conjugated forms. To gain insight into the enzymatic sulfation of tocopherols and their metabolites, a systematic investigation was performed using all 14 known human cytosolic sulfotransferases (SULTs). The results showed that the members of the SULT1 family displayed stronger sulfating activities toward tocopherols and their metabolites. These enzymes showed a substrate preference for γ-tocopherol over α-tocopherol and for γ-CEHC over other CEHCs. Using A549 human lung epithelial cells in a metabolic labeling study, a similar trend in the sulfation of tocopherols and CEHCs was observed. Collectively, the results obtained indicate that SULTmediated enzymatic sulfation of tocopherols and their metabolites is a significant pathway for regulation of the homeostasis and physiological functions of these important compounds.
DOI： 10.1271/bbb.110352