論文 - 山﨑 正夫
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Yamasaki M., Kitagawa T., Chujo H., Koyanagi N., Nishida E., Nakaya M., Yoshimi K., Maeda H., Nou S., Iwata T., Ogita K., Tachibana H., Yamada K.
Journal of Agricultural and Food Chemistry 52 ( 11 ) 3644 - 3648 2004年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Agricultural and Food Chemistry
Previous studies have shown the physiological significance of dietary conjugated linoleic acid (CLA) in various experimental animals and in human beings. One of the important problems to better elucidate is the difference between triglyceride (TG) and free (FFA) dietary CLA. Here, using splenocytes, this study assesses how TG- and FFA-CLA modulate immunoglobulin and various cytokine productions. In this study, C57BL/6N mice were fed an experimental diet containing 0% CLA, 0.1 or 1% FFA-CLA, or 0.1 or 1% TG-CLA for 3 weeks. The production of immunoglobulin tended to be up-regulated by 1% FFA-CLA. As a result of protein array analysis using the supernatant from splenocytes cultured with no CLA, 1% FFA-CLA, and TG-CLA, some cytokine production was shown to be remarkably regulated by dietary FFA- and TG-CLA. A total of 32 cytokines were examined, and 11-14 produced cytokines that were 2-fold up-regulated as compared with control for FFA- or TG-CLA, respectively. Especially, the production of IL-9 and MCP-5 and other cytokines was remarkably up-regulated by both FFA- and TG-CLA. In addition, seven cytokines were 2-fold down-regulated by TG-CLA. These data show that there is a slight but significant difference between the functionalities of FFA- and TG-CLA.
DOI： 10.1021/jf049929j
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Induction of apoptosis by tocotrienol in rat hepatoma dRLh-84 cells. 査読あり
M. Sakai, M. Okabe, M. Yamasaki, H. Tachibana, K. Yamada.
Anticancer Research 24 ( 3a ) 1683 - 1688 2004年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effect of dietary conjugated linoleic acid on liver regeneration after a partial hepatectomy in rats. 査読あり
A. Hirao, M. Yamasaki, H. Chujo, N. Koyanagi, H. Kanouchi, S. Yasuda, A. Matsuo, E. Nishida, E. Tsujita, T. Rikimaru, M. Shimada, Y. Maehara, H. Tachibana and K. Yamada.
Journal of Nutritional Science and Vitaminology 50 ( 1 ) 9 - 12 2004年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Dietary protein modulates the effect of CLA on lipid metabolism in rats 査読あり
A. Akahoshi., K. Koba., F. Ichinose., M. Kaneko., A. Shimoda., K. Nonaka., M. Yamasaki., T. Iwata., Y. Yamauchi., K. Tsutsumi., M. Sugano
Lipids 39 ( 1 ) 25 - 30 2004年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Chujo H., Yamasaki M., Nou S., Koyanagi N., Tachibana H., Yamada K.
Cancer Letters 202 ( 1 ) 81 - 87 2003年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cancer Letters
We evaluated the effect of conjugated linoleic acid (CLA) isomers on the growth factor-induced proliferation of human breast cancer MCF-7 cells. When MCF-7 cells were cultured in RPMI 1640 medium supplemented with 1% fetal bovine serum (FBS), CLA inhibited the proliferation and notably cis9, trans11 (c9,t11)-CLA showed the strongest effect. However, cells barely grew when cultured with 1% charcoal-treated FBS (cFBS). Proliferation was promoted in cFBS cultured cells by the addition of 17β-estradiol (E2), insulin, and epidermal growth factor (EGF). Trans10, cis12 (T10,c12)-CLA inhibited cell proliferation induced by E2 and insulin, but not by EGF. T10,c12-CLA also exhibited cell-killing activity when cells were induced with insulin. On the other hand, c9,t11-CLA was shown to have no effect on MCF-7 cell proliferation induced by and of these three growth factors. In conclusion, although both c9, t11 and t10, c12-CLA can inhibit the proliferation of MCF-7 cells, our results suggested that they have separate mechanisms and different targets of actions. © 2003 Elsevier Ireland Ltd. All rights reserved.
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Estrogenic compounds suppressed interferon-gamma production in mouse splenocytes through direct cell-cell interaction. 査読あり
M. Nakaya, M. Yamasaki, Y. Miyazaki, H. Tachibana, and K. Yamada.
In Vitro Cellular Develomental Biology-Animal 39 ( 8 ) 383 - 387 2003年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Antioxidant and anti-inflammatory effects of a diet supplemented with sesamin on hepatic ischemia-referfusion injury in rats. 査読あり
T. Utsunomiya, M. Shimada, T. Rikimaru, H. Hasegawa, Y. Yamashita, T. Hamatsu, M. Yamasaki, S. Kaku, K. Yamada and K.Sugimachi.
Hepato-Gastroenterology 50 ( 53 ) 1609 - 1613 2003年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Chujo H., Nou S., Tachibana H., Yamada K.
Cancer Letters 196 ( 2 ) 187 - 196 2003年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cancer Letters
We showed the inhibitory effect of oleic and palmitoleic acids (OA and POA) on this trans10, cis12 (10t, 12c)-CLA induced cytotoxic activity. When cells were cultured in the presence of 10t, 12c-CLA, this potent cytotoxic effect on dRLh-84 cells was clearly obvious when compared to the control vehicle group. It was revealed that the levels of cellular OA and POA levels decreased upon cis9, trans11 (9c,11t) or 10t, 12c-CLA treatment in a time course dependent manner. OA or POA demonstrates a dose dependent inhibition of the 10t, 12c-CLA induced cytotoxicity. Notable nuclear fragmentation or activation of caspase-3 and 9 by 10t, 12c-CLA in dRLh-84 was counteracted by treatment with OA or POA. Results also suggest that 10t, 12c-CLA induced apoptosis can be inhibited by treatment with OA or POA. © 2003 Elsevier Science Ltd. All rights reserved.
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共役脂肪酸の免疫調節機能 査読あり
山崎正夫、山田耕路
食品工業 46 ( 6 ) 31 - 35 2003年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Yamasaki M., Chujo H., Hirao A., Koyanagi N., Okamoto T., Tojo N., Oishi A., Iwata T., Yamauchi-Sato Y., Yamamoto T., Tsutsumi K., Tachibana H., Yamada K.
Journal of Nutrition 133 ( 3 ) 784 - 788 2003年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Nutrition
We evaluated the effect of cis-9, trans-11 (9c, 11t) and trans-10, cis-12 (10t, 12c) conjugated linoleic acid (CLA) on the immune system in C57BL/6J mice. Mice were fed experimental diets containing 0% CLA (controls), 1% 9c, 11t-CLA, 1% 10t, 12c-CLA or a 1:1 mixture (0.5% + 0.5%) of these two CLA isomers for 3 wk. Relative spleen weights of all CLA fed mice were greater than the controls. Spleen lymphocytes isolated from the mice fed 10t, 12c-CLA produced more immunoglobulin (Ig)A and IgM but not IgG when stimulated with concanavalin A (ConA) compared with controls. IgA production from unstimulated spleen lymphocytes was greater in the 10t, 12c-CLA group than in controls. Conversely, 9c, 11t-CLA did not affect the production of any of the Ig subclasses. Lymphocytes isolated from 9c, 11t-CLA fed mice produced more tumor necrosis factor-α than the control group. The proportion of B cells in the spleen lymphocyte population was significantly lower in the 9c, 11t-CLA group, and higher in the 10t, 12c-CLA group than in the controls. Compared with the control group, the percentage of CD4 + T cells was lower in the 10t, 12c-CLA group, and the percentage of CD8 + T cells was higher in the 9c, 11t-CLA group. Furthermore, the percentage of CD8 + T cells was higher in the 1:1 mixture group than in controls. The CD4 + /CD8 + ratio was lower in the 1:1 mixture group than in controls. These results suggest that 9c, 11t and 10t, 12c-CLA can stimulate different immunological effects and that the simultaneous intake of the two isomers can change the T cell population.
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Yamasaki M., Ikeda A., Oji M., Tanaka Y., Hirao A., Kasai M., Iwata T., Tachibana H., Yamada K.
Nutrition 19 ( 1 ) 30 - 35 2003年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nutrition
OBJECTIVES: In this study, we examined the effect of dietary conjugated linoleic acid (CLA) on body fat levels in Sprague-Dawley rats. Methods: Rats were fed AIN-93G type diets containing 4%, 7%, and 10% fats with or without 1.5% CLA. Results: Three weeks after the onset of the experimental period, the weights of perirenal white adipose tissue were lower in CLA-fed rats. The weights of epididymal white adipose tissue also were lower in CLA-fed rats than in control rats, but this effect disappeared with increased dietary fat level. Serum leptin levels tended to be lower in the CLA group, especially the low-fat diet group, than in the control group. There were significant positive correlations between serum leptin level and weights of perirenal and epididymal white adipose tissues in control groups, but these correlations were weaker in the CLA groups. Serum tumor necrosis factor-α levels also tended to be lower in CLA-fed rats, and this tendency was most remarkable in the rats fed 7% fat diets. Conclusions: In conclusion, dietary CLA, especially the low-fat diet, reduced body fat without hepatic injury to Sprague-Dawley rats. © Elsevier Science Inc. 2003.
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Yamasaki M., Chujo H., Koga Y., Oishi A., Rikimaru T., Shimada M., Sugimachi K., Tachibana H., Yamada K.
Cancer Letters 188 ( 1-2 ) 171 - 180 2002年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cancer Letters
We evaluated the cytotoxic effect of conjugated linoleic acid (CLA) on rat hepatoma dRLh-84 cells in vitro. When cells were cultured in the presence of CLA, strong cytotoxic effect on dRLh-84 cells was recognized at 1μM level compared to the control vehicle group, and trans10, cis12-CLA but not cis9, trans11-CLA was shown to be an active isomer for inducing this effect. Increase of the sub-G1 population and activation of caspase-3 and 9 accompanied with a time-dependent cleavage of poly(ADP-ribose) polymerase were recognized in dRLh-84 cells treated with trans10, cis12-CLA. In addition, we could see nuclear fragmentation in dRLh-84 cells treated with trans10, cis12-CLA by laser scanning confocal microscopy observation. Cytotoxic effect of trans10, cis12-CLA on normal hepatocytes was weaker than on dRLh-84 cells. These data indicate trans10, cis12-CLA has a potent cytotoxic effect on dRLh-84 cells through at least in part by an apoptotic pathway. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
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Inhibitory effect of conjugated linoleic acid isomers on the proliferation of human breast cancer cells
H. Chujo, M. Yamasaki, N. Koyanagi, H. Tachibana, and K. Yamada
Animal Cell Technology for creation of new era 13 465 - 470 2002年12月
記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス）
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Dose dependent effect of dietary conjugated linoleic acid on the growth of rat hepatoma dRLh-84 cells in vivo. 査読あり
M. Yamasaki, A. Ikeda, A. Hirao, Y. Tanaka, T. Rikimaru, M. Shimada, K. Sugimachi, H. Tachibana, and K. Yamada.
Journal of Nutritional Science and Vitaminology 48 ( 6 ) 505 - 511 2002年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Conjugated linoleic acid acid reduces body fats and cytokine level of mice. 査読あり
Akahoshi, Y. Goto, K. Murao, T. Miyazaki, M. Yamasaki, M. Nonaka, K. Yamada and M. Sugano
Bioscience Biotechnology Biochemistry 66 ( 4 ) 916 - 920 2002年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Dietary conjugated linolenic acid in relation to CLA differently modifies body fat mass and serum and liver lipid levels in rats. 査読あり
K. Koba, A. Akahoshi, M. Yamasaki, K. Tanaka, K. Yamada, T. Iwata, T. Kamegai, K. Tsutsumi, and M. Sugano.
Lipids 37 ( 4 ) 343 - 350 2002年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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共役リノール酸の免疫調節効果 招待あり
山崎正夫、山田耕路
日本食品化学工学会誌 49 ( 2 ) 130 - 134 2002年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Dietary manipulations of body fat-reducing potential of conjugated linoleic acid in rats 査読あり
M. Sugano, A. Akahoshi, K. Koba, K. Tanaka, T. Okumura, H. Matsuyama, Y. Goto, T. Miyazaki, K. Murao, M. Yamasaki, M. Nonaka, and Koji Yamada.
Bioscience Biotechnology Biochemistry 2001年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Oxidative stress by visible right irradiation suppresses immunoglobulin production in mouse spleen lymphocytes. 査読あり
Y. Miyazaki, M. Yamasaki, H. Mishima, K. Mansho, H. Tachibana, and K. Yamada
Bioscience Biotechnology Biochemistry 2001年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effect of dietary conjugated linoleic acid on the in vivo growth of rat hepatoma dRLh-84
Yamasaki M., Ikeda A., Hirao A., Tanaka Y., Miyazaki Y., Rikimaru T., Shimada M., Sugimachi K., Tachibana H., Yamada K.
Nutrition and Cancer 40 ( 2 ) 140 - 148 2001年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nutrition and Cancer
We examined the effect of dietary conjugated linoleic acid (CLA) on the growth of injected hepatoma dRLh-84 in Donryu rats. After experimental diets containing 0% or 2% CLA were given to male Donryu rats for 3 wk, dRLh-84 cells were injected into the left lobe of the hepatic capsule, and the experimental diet was continued. The cells formed a solid tumor ≥1 wk after the injection, and thereafter the tumor grew with feeding duration. In a morphological study, this tumor appeared to be a low-differentiated hepatoma, and there was no remarkable difference in the morphology of the tumor between 0% and 2% CLA groups. Tumor weight was significantly higher in the 2% CLA group than in the 0% CLA group throughout the feeding period after the injection. Serum glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase activities were significantly higher in 2% CLA-injected rats than in 0% CLA-injected rats at 3 wk after the injection. CLA upregulated acyl-CoA oxidase activity, especially 1 wk after the injection. However, dietary CLA did not activate carnitine palmitoyl transferase II, which is a rate-limiting enzyme in the mitochondrial β-oxidation pathway. Natural killer cell activity in the spleen tended to be higher in injected rats, but a significant effect of dietary CLA was not recognized. Serum interferon-γ and tumor necrosis factor-α levels were higher in injected than in sham rats. More-over, these levels were higher in 2% CLA groups than in the respective 0% CLA groups.