論文 - 松本 朋子
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ポルフィリン色素を用いる顕微吸光光度分析による固体酸の酸性度測定 査読あり
松本朋子,廣瀬大貴,松本 仁,白上 努,保田昌秀,笛田佳之
分析化学 61 851 - 856 2012年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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ポルフィリン色素を用いる顕微吸光光度分析による固体酸の酸性度測定
松本朋子, 廣瀬大貴, 松本 仁, 白上 努, 保田昌秀, 笛田佳之
分析化学 61 851 - 856 2012年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Matsumoto T., Hirose D., Yamauchi A., Matsumoto J., Shiragami T., Fueda Y., Yasuda M.
Bunseki Kagaku 61 ( 10 ) 851 - 856 2012年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bunseki Kagaku
The acidity of magnesium salts-silica gel composites was measured by absorption spectrophotometry in the micro-region using a confocal laser scanning microscope (CLSM). It has been reported that MgCl2-SiO2releases protons under dry conditions, and goes back to neutral under wet conditions. At first, using five kinds of tetraarylporphyrins, the absorbance (AP) at 515 nm due to a free base type and that (AG) at 650 nm due to the protonated porphyrins were measured in a CHCl3-MeOH-water solution, whose apparent pH was adjusted by HClO4. Fraction (F-value) of AP [F = AP/(AG+ AP)] was calculated at every apparent pH to produce apparent pH-profiles of the f-values for all porphyrins. The profiles were fitted by sigmoid curves. Second, the porphyrin was adsorbed on MgCl2-SiO2. The porphyrin- adsorbed composite was kept under the given relative humidity conditions for 3 days and subjected to CLSM analysis. The F-values of the porphyrins adsorbed on the composites were obtained from the absorption spectra to determine the apparent pH using fitting curves. As a result, the MgCl2-SiO2had an apparent pH of 1.73 under dry conditions, and 4.39 under wet conditions. Moreover, the pH values of the Mg(NO3)2-SiO2composite and the MgSO4-SiO2composite were measured. © 2012, The Japan Society for Analytical Chemistry. All rights reserved.
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Matsumoto Tomoko, Mitsumura Yoko, Miyamoto Miyuki, MATSUMOTO Jin, SHIRAGAMI Tsutomu, FUEDA Yoshiyuki, NOBUHARA Kazunori, YASUDA Masahide
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 27 ( 6 ) 623 - 628 2011年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japan Society for Analytical Chemistry
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Quantitative Analysis for a Color-Change of Humidity Indicator by Microscopic Absorption Spectrometry 査読あり
T. Matsumoto, Y. Mitsumura, M. Miyamoto, J. Matsumoto, T. Shiragami, Y. Fueda, K. Nobuhara, M. Yasuda,
Analytical Sciences 27 623 - 628 2011年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Quantitative Analysis for a Color-Change of Humidity Indicator by Microscopic Absorption Spectrometry
Tomoko Matsumoto, Yoko Mitsumura, Miyuki Miyamoto, Jin Matsumoto, Tsutomu Shiragami, Yoshiyuki Fueda, Kazunori Nobuhara, Masahide Yasuda
ANALYTICAL SCIENCES 27 ( 6 ) 623 - 628 2011年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：JAPAN SOC ANALYTICAL CHEMISTRY
A sensitive and easily distinguishable cobalt-free humidity indicator of porphyrin-silica gel-MgCl(2) composite was prepared from pH-induced spectra changeable tetraarylporphyrin, silica gel (SiO(2)), and MgCl(2). The pH change arose from proton release under dry conditions, and proton capture under humid conditions by a reversible reaction between MgCl(2) and a silanol group of SiO(2). A pink-orange porphyrin-Si(OH)(2)-MgCl(2) composite was dried to give a green protonated porphyrin-SiO(2)Mg composite. The optimized concentrations of MgCl(2) to make the concentrations of protonated porphyrin maximum under dry conditions were determined by absorption spectrometry of the green composite using a confocal laser scanning microscope as a microscopic spectrometer. Moreover, the green composite was prepared by heating dichloro(tetraarylporphyrinato)phosphorus chloride with MgCl(2) and SiO(2). The humidity-sensitivity of the green composite was evaluated by the absorption spectra under controlled humidity. A distinguishable color change of the green composite took place below 30% of relative humidity.
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共焦点レーザー顕微鏡を活用した微小領域分光分析法の開発と応用
松本 朋子
2011年4月
記述言語：日本語 掲載種別：学位論文（博士）
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Matsumoto J., Shinbara T., Tanimura S., Matsumoto T., Shiragami T., Yokoi H., Nosaka Y., Okazaki S., Hirakawa K., Yasuda M.
Journal of Photochemistry and Photobiology A: Chemistry 218 ( 1 ) 178 - 184 2011年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Photochemistry and Photobiology A: Chemistry
In an effort to develop water-soluble singlet-oxygen (1O 2) photosensitizer, we synthesized a series of phosphorus porphyrin complexes (1, (RO)2P(tpp)+Cl-, tpp = tetraphenylporphyrinato ligand, R = -(CH2CH2O) m-(CH2)n-H, m = 0-3, n = 1, 2, 4, 6) and evaluated their performance characteristics. Water-solubilities of 1 (m = 1, 2 and n = 1, 2, 4, 6) were determined to be >1.0 mM, which was higher than the parent (HO)2P(tpp)+Cl-. The quantum yields for the generation of 1O2 in water based on the amount of 11* were determined to be 0.73, 0.69, and 0.62 for 1d (m = 2, n = 4), 1e (m = 2, n = 2), and 1f (m = 2, n = 1), respectively. Photo-inactivation by 1 was performed with yeast (Saccharomyces cerevisiae), and was evaluated using a inactivation factor AF = ([1]M × T1/2)-1, where [1]M is the minimum effective concentration of 1 and T1/2 is a half-life of the yeast initially used. In the case of 1b (m = 1, n = 6) and 1c (m = 2, n = 6), [1] M was determined to be 5 nM in the photo-sterilization. Bio-affinity was evaluated by the adsorbed concentration of 1 ([P]ad) into the yeast, which was measured by absorption spectrophotometry using a confocal laser-scanning microscope. These findings revealed that a large amount of 1 was adsorbed into the yeast. The estimated amount of 1b obtained from 10 μM aqueous solution came to 171 mM (17,100 fold) in terms of concentration. As an increase in carbon number (n) of the alkyl group on axial ligands, AF and [P]ad increased. It is strongly suggested that the photo-inactivation took place with the assistance of the attack of 1O2 inside the cells. © 2011 Elsevier B.V.
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Water-soluble phosphorous porphyrins with high activity for visible light-assisted inactivation of Saccharomyces cerevisiae 査読あり
J. Matsumoto, T. Shinbara, S. Tanimura, T. Matsumoto, T. Shiragami, H. Yokoi, Y. Nosaka, S. Okazaki, K. Hirakawa, M.Yasuda
Journal of Photochemistry and Photobiology A: Chemistry 218 178 - 184 2011年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Water-soluble phosphorous porphyrins with high activity for visible light-assisted inactivation of Saccharomyces cerevisiae
J. Matsumoto, T. Shinbara, S. Tanimura, T. Matsumoto, T. Shiragami, H. Yokoi, Y. Nosaka, S. Okazaki, K. Hirakawa, M.Yasuda
Journal of Photochemistry and Photobiology A: Chemistry 218 178 - 184 2011年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Matsumoto T., Mitsumura Y., Miyamoto M., Matsumoto J., Shiragami T., Fueda Y., Nobuhara K., Yasuda M.
Analytical Sciences 27 ( 6 ) 623 - 628 2011年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Analytical Sciences
A sensitive and easily distinguishable cobalt-free humidity indicator of porphyrin-silica gel-MgCl 2 composite was prepared from pH-induced spectra changeable tetraarylporphyrin, silica gel (SiO 2 ), and MgCl 2 . The pH change arose from proton release under dry conditions, and proton capture under humid conditions by a reversible reaction between MgCl 2 and a silanol group of SiO 2 . A pink-orange porphyrin-Si(OH) 2 -MgCl 2 composite was dried to give a green protonated porphyrin-SiO 2 Mg composite. The optimized concentrations of MgCl 2 to make the concentrations of protonated porphyrin maximum under dry conditions were determined by absorption spectrometry of the green composite using a confocal laser scanning microscope as a microscopic spectrometer. Moreover, the green composite was prepared by heating dichloro(tetraarylporphyrinato)phosphorus chloride with MgCl 2 and SiO 2 . The humidity-sensitivity of the green composite was evaluated by the absorption spectra under controlled humidity. A distinguishable color change of the green composite took place below 30% of relative humidity. 2011 © The Japan Society for Analytical Chemistry.
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Matsumoto T., Nakahara T., Matsumoto J., Shiragami T., Yasuda M.
Bunseki Kagaku 58 ( 5 ) 357 - 361 2009年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bunseki Kagaku
An evaluation of the bio-affinity of methoxo(alkyloxo)-tetraphenylporphyrinatoantimony bromide (1) was examined by an absorption experiment of 1 toward Saccharomyces cerevisiae. An aqueous solution of 1 was added to an aqueous solution of S. cerevisiae. Then an aqueous solution of agar was added into the solution in order to stop the Brownian motion of bacteria during analysis. A prepared aqueous solution containing 1(10 μmol dm-3), S. cerevisiae (1.0 ×104 cells cm-3) and agar (0.4 wt%) was taken inside a space surrounded by a silicone spacer (1 cm ×1 cm, thickness 50 μm) put on a slide glass, which was set on a confocal laser scanning microscope (CLSM) stage. By measuring the diameter of the cell and the absorbance on CLSM, the concentrations of 1 adsorbed inside S. cerevisia were determined to be 54.2 ±7.3 for 1a, 49.8 ±6.2 for 1b, and 22.5 ±15.5 mmol dm-3 for 1c according to Lambert-Beer's law. © 2009 The Japan Society for Analytical Chemistry.
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Visible light-assisted sterilization activity of water-soluble antimonyporphyrin toward Saccharomyces cerevisiae 査読あり
M. Yasuda, T. Nakahara, T. Matsumoto, T. Shiragami, J. Matsumoto, H. Yokoi, T. Hirano, K. Hirakawa
Journal of Photochemistry and Photobiology A: Chemistry 205 210 - 214 2009年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Visible light-assisted sterilization activity of water-soluble antimonyporphyrin toward Saccharomyces cerevisiae
M. Yasuda, T. Nakahara, T. Matsumoto, T. Shiragami, J. Matsumoto, H. Yokoi, T. Hirano, K. Hirakawa
Journal of Photochemistry and Photobiology A: Chemistry 205 210 - 214 2009年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Yasuda M., Nakahara T., Matsumoto T., Shiragami T., Matsumoto J., Yokoi H., Hirano T., Hirakawa K.
Journal of Photochemistry and Photobiology A: Chemistry 205 ( 2-3 ) 210 - 214 2009年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Photochemistry and Photobiology A: Chemistry
Sterilization activities of seven kinds of water-soluble dialkyloxo(tetraphenylporphyrinato)antimony(V) bromides (1) were examined against a yeast, Saccharomyces cerevisiae, under visible light irradiation. Sterilization activities were analyzed by the first-order kinetics involving the generation of singlet oxygen (1O2) in the complex between 1 and the cells. The formation of the complexes was confirmed by the measurements of the saturated adsorption concentrations of 1 inside yeast by absorption spectrophotometry using a confocal laser scanning microscope. The quantum yields of 1 for the generation of 1O2 were determined to be 0.48-0.65. In order to ensure efficient sterilization, the requisite was that 1 were both water-soluble and oil-soluble. Presumably the oil-soluble feature of 1 was advantageous in passing through the cell wall of the yeast which consisted of hydrophobic peptideglucan and the water-soluble feature was advantageous in the adsorption occurring at hydrophilic sites inside the cell, causing fatal damage to the yeast. © 2009 Elsevier B.V. All rights reserved.
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共焦点レ-ザー走査型顕微鏡を用いる酵母菌体へ吸着した水溶性ポルフィリン誘導体の吸光光度定量 査読あり
松本 朋子，中原 卓郎，松本 仁，白上 努，保田 昌秀
分析化学 58 357 - 361 2009年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Microscopic spectrophotometry using confocal laser scanning microscope
Matsumoto T., Shiraki R., Matsumoto J., Shiragami T., Yasuda M.
Bunseki Kagaku 57 ( 10 ) 819 - 824 2008年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bunseki Kagaku
In order to elucidate the applicable range and accuracy of absorption spectrophotometry in the micro-region using a confocal laser scanning microscope (CLSM), micro-silica gel beads (2) immobilizing a given molar concentration (C1m) of dihydroxo(tetraphenylporphyrinato)-antimony(V) bromide (1) were prepared. The measurable area on the beads was determined to be a circle of 2.14 μm diameter by magnifications of an objective lens, the diameter of fiber core, and the diameter of a pinhole. Moreover, the range of the diameter of the beads and the spectral range were decided to be μm ∼ 350 μm and 488-775 nm, respectively. Using a halogen lamp as a light source on CLSM, the molar concentrations (C2) of 1 in 2 were determined by absorption spectrophotometry. The value of C2 showed good agreement with C1m with 0.11 -0.73 of the standard deviation. Thus, absorption spectrophotometry in the micro-region will be a powerful tool for the quantitative analysis of transparent materials of micron-order. © 2008 The Japan Society for Analytical Chemistry.
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共焦点レーザー走査型顕微鏡を用いる顕微吸光度分析 査読あり
松本 朋子，白木 隆一，松本 仁，白上 努，保田 昌秀
分析化学 57 819 - 824 2008年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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共焦点レーザー走査型顕微鏡を用いる顕微吸光度分析
松本 朋子, 白木 隆一, 松本 仁, 白上 努, 保田 昌秀
分析化学 57 819 - 824 2008年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Preparation and characterization of porphyrin chromophores immobilized on micro-silica gel beads
Matsumoto J., Matsumoto T., Senda Y., Shiragami T., Yasuda M.
Journal of Photochemistry and Photobiology A: Chemistry 197 ( 1 ) 101 - 109 2008年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Photochemistry and Photobiology A: Chemistry
Micro-silica gel beads (1a-c) immobilizing dihydroxoantimony(V) tetraphenylporphyrin (SbTpp), free base tetraphenylporphyrin (H2Tpp), and zinc(II) tetraphenylporphyrin (ZnTpp) chromophores were prepared by the reactions of 3-aminopropyl silica gel (SiO2-NH2) with the dihydroxoantimony(V) complex (2a), the free base (2b), and the zinc complex (2c) of 5-[4-(succinimidyloxycarbonyl)phenyl]-10,15,20-triphenylporphyrin, respectively. Absorption spectrophotometry was performed on 1a-c using a confocal laser scanning microscope (CLSM) and determined that the yields for the immobilization of SbTpp, H2Tpp, and ZnTpp on SiO2-NH2 were 31.7, 95.9, and 45.1%, respectively. Moreover, 1a was converted into its acetyl analog (1a') by the acetylation of the axial hydroxo ligands and aminopropyl of 1a with Ac2O, since the interactions of the SbTpp chromophore with the residual amino group on SiO2 was observed by spectroscopic analysis using CLSM. In micro-channel reactors (MCR), the photoreaction of 1a' with Et2NH induced demetallation to produce 1b immobilizing H2Tpp chromophore. Moreover, the reaction of 1b with Zn(OAc)2 produced 1c immobilizing ZnTpp chromophore. Thus, micro-silica gel beads (1a) were transformed into 1c in a micro-region of a silica gel surface by way of photochemical reaction with Et2NH and subsequent metallation with Zn(OAc)2. © 2008 Elsevier B.V. All rights reserved.