論文 - 明石 良
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Effect of palm kernel cake as protein source in a concentrate diet on intake, digestibility and live weight gain of goats fed Napier grass. 査読あり
Rahman M.M., Abdullah R.B., Wan Embong W.K., Nakagawa T., Akashi R.
Tropical Animal Health and Production 2012年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effect of palm kernel cake as protein source in a concentrate diet on intake, digestibility and live weight gain of goats fed Napier grass 査読あり
3. Rahman M.M., Abdullah R.B., Wan Embong W.K., Nakagawa T., Akashi R
Tropical Animal Health and Production 45 873 - 878 2012年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Estimation of genome size of jute (Corchorus capsularis (L.) var. CVL-1 using flow cytometry 査読あり
Akashi R., Fancy N., Tanmoy A., Khan H.
Plant Tissue Culture and Biotechnology 22 ( 1 ) 83 - 86 2012年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plant Tissue Culture and Biotechnology
Genome size is an informative biological marker in understanding its complexity. Moreover, selection of breeding material(s) largely depends on the size of the parental genome. In this study, genome size of one of the most important natural fibers, jute (Corchorus capsularis) is reported. Flow cytometry was used, since it can provide an accurate estimation. The estimated size of the haploid genome (1C) of jute (Corchorus capsularis var. CVL-1) was found to be 274±10.7 MbpC -1 . This information should be helpful to shed more light on the evolutionary history of the genus Corchorus regarding their phylogenetic relationships and chromosomal evolutions as well as selection of breeding material(s) for hybridization.
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Additional cause for reduced JA-Ile in the root of a Lotus japonicus phyB mutant. 査読あり
Shigeyama T., Tominaga A., Arima S., Sakai T., Inada S., Jikumaru Y., Kamiya Y., Uchiumi T., Abe M., Hashiguchi M., Akashi R., Hirsch A.M., Suzuki A.
Plant Signaling Behavior 7 746 - 748 2012年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Additional cause for reduced JA-Ile in the root of a Lotus japonicus phyB mutant. 査読あり
Shigeyama T., Tominaga A., Arima S., Sakai T., Inada S., Jikumaru Y., Kamiya Y., Uchiumi T., Abe M., Hashiguchi M., Akashi R., Hirsch A., Suzuki A.
Plant signaling & behavior 7 ( 7 ) 746 - 748 2012年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plant signaling & behavior
Light is critical for supplying carbon for use in the energetically expensive process of nitrogen-fixing symbiosis between legumes and rhizobia. We recently showed that root nodule formation in phyB mutants [which have a constitutive shade avoidance syndrome (SAS) phenotype] was suppressed in white light, and that nodulation in wild-type is controlled by sensing the R/FR ratio through jasmonic acid (JA) signaling. We concluded that the cause of reduced root nodule formation in phyB mutants was the inhibition of JA-Ile production in root. Here we show that the shoot JA-Ile level of phyB mutants is higher than that of the wild-type strain MG20, suggesting that translocation of JA-Ile from shoot to root is impeded in the mutant. These results indicate that root nodule formation in phyB mutants is suppressed both by decreased JA-Ile production, caused by reduced JAR1 activity in root, and by reduced JA-Ile translocation from shoot to root.
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Additional cause for reduced JA-Ile in the root of a Lotus japonicus phyB mutant 査読あり
Shigeyama T., Tominaga A., Arima S., Sakai T., Inada S., Jikumaru Y., Kamiya Y., Uchiumi T., Abe M., Hashiguchi M., Akashi R., Hirsch A., Suzuki A.
Plant Signaling and Behavior 7 ( 7 ) 1 - 3 2012年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plant Signaling and Behavior
Light is critical for supplying carbon for use in the energetically expensive process of nitrogen-fixing symbiosis between legumes and rhizobia. We recently showed that root nodule formation in phyB mutants [which have a constitutive shade avoidance syndrome (SAS) phenotype] was suppressed in white light, and that nodulation in wild-type is controlled by sensing the R/FR ratio through jasmonic acid (JA) signaling. We concluded that the cause of reduced root nodule formation in phyB mutants was the inhibition of JA-Ile production in root. Here we show that the shoot JA-Ile level of phyB mutants is higher than that of the wild-type strain MG20, suggesting that translocation of JA-Ile from shoot to root is impeded in the mutant. These results indicate that root nodule formation in phyB mutants is suppressed both by decreased JA-Ile production, caused by reduced JAR1 activity in root, and by reduced JA-Ile translocation from shoot to root. © 2012 Landes Bioscience.
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Estimation of genome size of Jute (Corchorus capsularis (L.) var. CVL-1 using flow cytometry. 査読あり
Akashi R., Fancy N.N., Tanmoy A.M., Khan H.
Plant Tissue Culture & Biotechnology 22 83 - 86 2012年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Quantitative trait locus analysis of symbiotic nitrogen fixation activity in the model legume Lotus japonicus. 査読あり
Tominaga A., Gondo T., Akashi R., Zheng S.H., Arima S., Suzuki A.
Journal of Plant Research 125 395 - 406 2012年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Fertile transgenic Brachiaria ruziziensis (ruzigrass) plants by particle bombardment of tetraploidized callus. 査読あり
Ishigaki G., Gondo T., Suenaga K., Akashi R.
Journal of Plant Physiology 169 546 - 549 2012年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Ishigaki G., Gondo T., Suenaga K., Akashi R.
Journal of Plant Physiology 169 ( 5 ) 546 - 549 2012年3月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Plant Physiology
We have produced transgenic plants of the tropical forage crop Brachiaria ruziziensis (ruzigrass) by particle bombardment-mediated transformation of multiple-shoot clumps and embryogenic calli. Cultures of multiple-shoot clumps and embryogenic calli were induced on solidified MS medium supplemented with 0.5. mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 2. mg/L 6-benzylaminopurine (BAP) or 4. mg/L 2,4-D and 0.2. mg/L BAP, respectively. Both cultures were bombarded with a vector containing an herbicide resistance gene (bar) as a selectable marker and the β-glucuronidase (GUS) reporter gene. Sixteen hours after bombardment, embryogenic calli showed a significantly higher number of transient GUS expression spots per plate and callus than multiple-shoot clumps, suggesting that embryogenic callus is the more suitable target tissue. Following bombardment and selection with 10. mg/L bialaphos, herbicide-resistant embryogenic calli regenerated shoots and roots in vitro, and mature transgenic plants have been raised in the greenhouse. Polymerase chain reaction (PCR) and DNA gel blot analysis verified that the GUS gene was integrated into the genome of the two regenerated lines. In SacI digests, the two transgenic lines showed two or five copies of GUS gene fragments, respectively, and integration at different sites. Histochemical analysis revealed stable expression in roots, shoots and inflorescences. Transgenic plants derived from diploid target callus turned out to be sterile, while transgenics from colchicine-tetraploidized callus were fertile. © 2011 Elsevier GmbH.
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The National BioResource Project (NBRP) Lotus and Glycine in Japan. 査読あり
Hashiguchi M., Abe J., Aoki T., Anai T., Suzuki A., Akashi R.
Breeding Science 61 453 - 461 2012年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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宮崎県のスイートピー生産者における生産費，作業および省力形質に対する意識調査 査読あり
中村 薫, 福元 孝一, 郡司 定雄, 明石 良
園芸学研究 11 ( 4 ) 439 - 447 2012年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：園芸学会
省力的形質とされる無巻きひげ形質を持つスイートピー切り花品種の育成の有望性を検討するため，生産者に対し2009年にアンケート調査を実施した．調査対象は宮崎県の97戸の経営主と家族専従者とし，設問は生産規模，最も注意する生産費，最も労力を必要とすると思われる作業項目，巻きひげの必要性，無巻きひげ品種の作付けに対する意向および望ましい品種の形質とした．回答が得られた46人の経営主の平均栽培面積は17.3 aであった．栽培従事者1人当たりの栽培面積は3.2 aであり，栽培面積と栽培従事者数の関係は有意な正の相関を示した（r = 0.91**）．78人の回答者を経営主か家族専従者か，雇用の有無，栽培面積の大きさという3つのカテゴリーで分けて集計を行った．最も注意する生産費では，経営主と家族専従者の意識に差は無く，「雇用費」が最も多く選択されたが，雇用を有しない生産者および栽培面積が10 a未満の生産者では「出荷経費」が最も多く選択され，雇用を有する生産者および10 a以上の面積の生産者では「雇用費」であった．最も労力が必要と思われる作業項目は「誘引」と「つる下げ」が，巻きひげの必要性では「どちらでもかまわない」が回答者の属性にかかわらず最も多く選択された．無巻きひげ品種の作付けに対する意識では，経営主では「導入する品種候補に入れる」が最も多く選択され，経営主が省力効果に期待していることが明らかになった．望ましいと考える品種の形質では経営主，家族専従者とも「難落蕾」，「花持ち」，「高規格率」および「高収量」が「省力」よりも多く選択された．<br>
DOI： 10.2503/hrj.11.439
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わい性ネピアグラスにおける腋芽生長点からの多芽体形成と効率的な苗生産 査読あり
Nafiatul UMAMI, 権藤 崇裕, 石垣 元気, Mohammad Mijanur RAHMAN, 明石 良
日本暖地畜産学会報 55 ( 2 ) 121 - 127 2012年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本暖地畜産学会
本研究は，わい性ネピアグラスにおける効率的な苗生産を行うための組織培養法の改善を図り，また，苗生産において重要な課題である順化の効率化やソマクローナル変異の誘発などの問題を軽減したものである．外植体として用いた腋芽は，分げつから無菌的に摘出して，2,4-DおよびBAPを組み合わせて添加したMS固形培地で多芽体を誘導した．その結果，多芽体は0.1 mg L<sup>-1 </sup>2,4-Dおよび2.0 mg L<sup>-1 </sup>BAP添加の培地で29%と最も高い誘導率を示した．培養培地への硫酸銅の添加は，多芽体の増殖率を向上し，50 μMの硫酸銅の添加が増殖に最も有効であった．増殖した多芽体は，0.1 mg L<sup>-1 </sup>NAAおよび2.0 mg L<sup>-1 </sup>BAP添加のMS固形培地で84％と最も高い再分化率を示し，多くの植物体が再分化した．また，再分化植物体の全ては順化することができ，土壌中で旺盛に生長した．再分化植物体および栄養苗から生長させた植物体について形態を調査したところ，組織培養による形態的な変異は認められず，両者間に統計的な有意差（5％水準）はなかった．また，フローサイトメーターによる倍数性の調査では，再分化植物体における染色体数の倍加は認められなかった．以上のことから，本研究で改良した培養法により，わい性ネピアグラスの高品質な苗生産技術を確立できたものと判断した．
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ISS education program "JAXA seeds in space I"" 査読あり
Nakano T., Hirohama E., Ogawa S., Tanigaki F., Abe T., Hayashi Y., Nitasaka E., Akashi R., Hashiguchi M., Aoki T.
62nd International Astronautical Congress 2011, IAC 2011 11 8833 - 8843 2011年12月
記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス） 出版者・発行元：62nd International Astronautical Congress 2011, IAC 2011
In order to have students experience the Science, participate in the real scientific investigation, we have begun the "seeds in space" education programs. Concretely, the main purpose of JAXA Student experiments is for students to learn the mutation effects of space-flight on plant seeds. In March 2008, seeds of Asagao (Japanese morning glory), Miyako-gusa (Japanese bird's foot trefoil) and Sunflower were launched to the International Space Station (ISS) aboard Endeavour Shuttle Mission STS-123. The specimens were then remained on the ISS for near 9 months, when Endeavour Shuttle Mission STS-126 retrieved these educational biosamples, the seeds were returned to Earth. In case plant seeds were exposed to radiation as mutagen, the resulting mutations would be recessive in almost all examples. In breeding science, the above views are commonly well known. So, one could not identify mutants in the first generation cultivated from space-flight seeds judged by the observation of their phenotypes such as the figures, colors and so on. However, in the second generation, we can easily find the recessive homozygote as a mutant from their looks, if the plant has the characteristics of not only diploid but also self-pollination. Although there are also many types of plants characteristic of polyploidy, or cross pollination, the criteria described above could not be applied to them at all. It is summarized as follows: we have chosen seeds of Asagao and Miyako-gusa featuring with diploid and self-pollination as biospecimens for this education experiment, which could be used to identify the mutants from their phenotypes on the M2 generation. The space-flight seeds of Asagao and Miyako-gusa were then distributed to some hundred of schools in Japan, on May 2010 with another sets of ground (negative)-control (i.e., seeds that never left Earth) and positive control (seeds irradiated with carbon ion beams at RIKEN RI-Beam Factory). Participating students aged 3 to 18 year-old were given every 10 seeds of flight, ground control and positive control as one set for Asagao and, for Miyako-gusa, 20 every seeds were distributed. The students have been still conducting the cultivation of their plants twice for two seasons to find the mutants until fall of 2011. It is our hope that the students will precisely understand that the mutation rates of seeds in such a space-flight event, are extremely low compared to their expectations, and also they will actually learn what scientific experiments are.
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Lotus japonicus nodulation is photomorphogenetically controlled by sensing the red/far red (R/FR) ratio through jasmonic acid (JA) signaling. 査読あり
Suzuki A., Suriyagoda L., Shigeyama T., Tominaga A., Sasaki M., Hiratsuka Y., Yoshinaga A., Arima S., Agarie S., Sakai T., Inada S., Jikumaru Y., Kamiya Y., Uchiumi T., Abe M., Hashiguchi M., Akashi R., Sato S., Kaneko T., Tabata S., Hirsch A.M.
Proceedings of the National Academy of Sciences of the United States of America 108 16837 - 16842 2011年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Suzuki A., Suriyagoda L., Shigeyama T., Tominaga A., Sasaki M., Hiratsuka Y., Yoshinaga A., Arima S., Agarie S., Sakai T., Inada S., Jikumaru Y., Kamiya Y., Uchiumi T., Abe M., Hashiguchi M., Akashi R., Sato S., Kaneko T., Tabata S., Hirsch A.
Proceedings of the National Academy of Sciences of the United States of America 108 ( 40 ) 16837 - 16842 2011年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Proceedings of the National Academy of Sciences of the United States of America
Light is critical for supplying carbon to the energetically expensive, nitrogen-fixing symbiosis between legumes and rhizobia. Here, we show that phytochrome B (phyB) is part of the monitoring system to detect suboptimal light conditions, which normally suppress Lotus japonicus nodule development after Mesorhizobium loti inoculation. We found that the number of nodules produced by L. japonicus phyB mutants is significantly reduced compared with the number produced of WT Miyakojima MG20. To explore causes other than photoassimilate production, the possibility that local control by the root genotype occurred was investigated by grafting experiments. The results showed that the shoot and not the root genotype is responsible for root nodule formation. To explore systemic control mechanisms exclusive of photoassimilation, we movedWTMG20 plants from white light to conditions that differed in their ratios of low or high red/far red (R/FR) light. In low R/FR light, the number of MG20 root nodules dramatically decreased compared with plants grown in high R/FR, although photoassimilate content was higher for plants grown under low R/FR. Also, the expression of jasmonic acid (JA) -responsive genes decreased in both low R/FR light-grownWT and white light-grown phyB mutant plants, and it correlated with decreased jasmonoyl-isoleucine content in the phyB mutant. Moreover, both infection thread formation and root nodule formation were positively influenced by JA treatment of WT plants grown in low R/FR light and white light-grown phyB mutants. Together, these results indicate that root nodule formation is photomorphogenetically controlled by sensing the R/FR ratio through JA signaling.
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Morphological traits of Lotus japanicus(Regal) ecotypes collected in Japan. 査読あり
10. Hashiguchi M., Tsuruta S., Akashi R.
Interdiscipinary Bio Central 3 1 - 7 2011年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Discovery of natural Miscanthus (Poaceae) triploid plants in sympatric populations of Miscanthus sacchariflorus and Miscanthus sinensis in southern Japan 査読あり
Nishiwaki A, Mizuguti A, Kuwabara S, Toma Y, Ishigaki G, Miyashita T, Yamada T, Matuura H, Yamaguchi S, Lane Rayburn A, Akashi R, Ryan Stewart J
American Journal of Botany 98 154 - 159 2011年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Asanuma Y., Jinkawa T., Tanaka H., Gondo T., Zaita N., Akashi R.
Transgenic Research 20 ( 1 ) 91 - 97 2011年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Transgenic Research
Environmental risk assessment of transgenic crops is implemented under the Cartagena Protocol domestic law in accordance with guidelines for implementing the assessment established by the Ministry of Agriculture, Forestry and Fisheries (MAFF) and the Ministry of Environment (MOE) in Japan. Environmental risk assessments of transgenic crops are implemented based on the concept of 'substantial equivalence' to conventional crops. A unique requirement in Japan to monitor the production of harmful substances, or allelochemicals, is unparalleled in other countries. The potential for allelochemicals to be secreted from the roots of transgenic crops to affect other plants or soil microflora or for substances in the plant body to affect other plants after dying out must be evaluated. We evaluated the allelopathic potential of seven transgenic oilseed rape (Brassica napus L.) lines that express glufosinate tolerance in terms of substantial equivalence to conventional oilseed rape lines, and established evaluation methods. Our results indicate no potential production of allelochemicals for any of the seven transgenic oilseed rape lines compared with conventional oilseed rape lines. © 2010 Springer Science+Business Media B.V.
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Tsuruta Shin-ichi, Ebina Masumi, Kobayashi Makoto, AKASHI Ryo, KAWAMURA Osamu
Breeding science 60 ( 4 ) 314 - 323 2010年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japanese Society of Breeding