論文 - 明石 良
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Use of green fluorescent protein to trace the N2-fixing endophytic bacterium Herbaspirillum inoculated in a sugarcane plant. 査読あり
Shimizu T, Toyosato M, Kutsuna Y, Saeki Y, Minamizawa K, Nakanishi Y, Prisca NJ, Akashi R, Akao S
Soil Microorganisms 60 3 - 9 2006年4月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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ＤＮａｓｅ Ｉを用いた精液・ちつ液混合体液斑からの精子ＤＮＡ抽出法 査読あり
内山 岳人, 久澄 倫之介, 前田 育子, 中田 大仁, 大野 里香, 長谷川 哲朗, 清水 健史, 明石 良
日本法科学技術学会誌 11 ( 1 ) 105 - 112 2006年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本法科学技術学会
Typing of sperm DNA found in vaginal fluid is an important component of investigations of sexual crimes. A standard, two-step differential extraction of sperm and vaginal epithelial cells from vaginal fluid mixed with semen is usually used for DNA isolation. However, this method is considerably time-consuming and, due to often insufficient separation of sperm and vaginal pithelial DNA in the centrifugation process, DNA contamination may occur. Therefore, we have developed a new and more reliable method of sperm DNA extraction from vaginal fluid. Using a three-step, complete digesion of vaginal fluid with deoxyribonuclease I (DNase I) we were able to isolate pure sperm DNA in a shorter time. It can be expected that this improved method of sperm DNA isolation will lead to more reliable DNA typing and, significantly improve the efficiency of scientific criminal investigation such as DNA typing.
DOI： 10.3408/jafst.11.105
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Genetic diversity in zoysiagrass ecotypes based on morphological characteristics and SSR markers 査読あり
Hashiguchi M., Tsuruta S., Matsuo T., Ebina M., Kobayashi M., Akamine H., Akashi R.
Molecular Breeding for The Genetic Improvement of Forage Crops and Turf: Proceedings of the 4th International Symposium on The Molecular Breeding of Forage and Turf, a Satellite Workshop of the XXth International Grassland Congress, July 2005, Aberystwyth, Wales 2005年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Molecular Breeding for The Genetic Improvement of Forage Crops and Turf: Proceedings of the 4th International Symposium on The Molecular Breeding of Forage and Turf, a Satellite Workshop of the XXth International Grassland Congress, July 2005, Aberystwyth, Wales
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Development and characterization of simple sequence repeat markers in Zoysia japonica Steud 査読あり
Tsuruta Shin-Ichi, Hashiguchi Masatsugu, Ebina Masumi, MATSUO Taiyo, YAMAMOTO Toshiya, KOBAYASHI Makoto, TAKAHARA Manabu, NAKAGAWA Hitoshi, AKASHI Ryo
Grassland science 51 ( 3 ) 249 - 257 2005年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japanese Society of Grassland Science
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Green, herbicide-resistant plants by particle inflow gun-mediated gene transfer to diploid bahiagrass (Paspalum notatum) 査読あり
共著：Gondo T., S. Tsuruta, R. Akashi, O. Kawamura , F. Hoffmann
Journal of Plant Physiology 162(8), 864-872 2005年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Development and characterization of simple sequence repeat markers in Zoysia japonica Steud. 査読あり
共著：S.Tsuruta, M. Hashiguchi, M.Ebina, T.Matsuo, T. Yamamoto, M. Kobayashi, M. Takahara, H. Nakagawa, R. Akashi
Grassland Science 51: 249-257 2005年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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西洋ミヤコグサ由来"Super-growing Root" (Lotus corniculatus L.)の発根・伸長に及ぼす亜鉛添加の効果 査読あり
共著者：小口慶子・井上昭彦・明石 良・佐伯雄一・長友由隆・赤尾勝一郎・高木 浩
日本土壌肥料学雑誌 75(1), 9-14 2004年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Nutritive evaluation of forage plants grown in South Sulawesi, Indonesia. 2. Mineral composition. 査読あり
Nasurula, Niimi M, Akashi R, Kawamura O
Asian-Australasian Journal of Animal Sciences 17 63 - 67 2004年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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BAPおよび硫酸銅がバヒアグラス（Paspalum notatum Flugge）の種子由来エンブリオジェニックカルス形成と植物体再分化に及ぼす効果
共著者：権藤崇裕，明石 良，川村修
Grassland Science,50(1): 55-58 2004年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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霍田 真一, 蝦名 真澄, 橋口 正嗣, 松尾 太陽, 明石 良, 高原 学, 小林 真
日本草地学会誌 50 262 - 263 2004年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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久綱 泰代, 繁木 雄一, 明石 良
日本草地学会誌 50 516 - 517 2004年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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ローズグラス (Chloris gayana) における遺伝子組換え体の作出 査読あり
松元 潤, 権藤 崇裕, 明石 良
日本草地学会誌 50 284 - 285 2004年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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バヒアグラスの組織培養における硫酸銅添加培地での連続的な不定胚形成 査読あり
権藤 崇裕, 山川 一富, 明石 良, 川村 修
日本草地学会誌 50 494 - 495 2004年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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バヒアグラス(Paspalum notatum Flugge)の懸濁培養細胞由来再分化個体における化学組成およびin vitro消化率 査読あり
Saleng Kamaruddin, 新美 光弘, 明石 良, ほか1名
宮崎大学農学部研究報告 50 ( 1 ) 25 - 30 2004年3月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：宮崎大学農学部
バヒアグラス(品種 Pensacola)の懸濁培養細胞由来再分化個体における細胞壁構成物質含量およびルーメン微生物によるin viro消化率を調査した。再分化個体群のNDF含量は、一般に、種子からの植物体（対照個体群）より高かった。リグニン含量には、両個体群の間で、統計的に有意な差がなかった。in viro乾物およびNDF消化率においては、再分化個体群が対照個体群より高かった。これらの結果は、組織培養が暖地型牧草の品質改善のための育種操作として有用であることを示唆している。
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Nutritive Evaluation of Forage Plants Grown in South Sulawesi, Indonesia II. Mineral Composition 査読あり
Nasrullah, Niimi M., Akashi R., Kawamura O.
Asian-Australasian Journal of Animal Sciences 17 ( 1 ) 63 - 67 2004年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Asian-Australasian Journal of Animal Sciences
In order to evaluate the nutritive value of the forage plants in South Sulawesi, Indonesia, 266 samples (61 grasses and 65 legumes grown in the dry season, 60 grasses and 80 legumes grown in the rainy season) were collected from the highland and the lowland in 1998 to 2000, and were subjected to the determination of mineral composition. The mean contents of Ca, Mg, P, Na and K in grasses were 0.6, 0.3, 0.5, 0.1 and 2.3%, respectively, and in legumes were 1.8, 0.5, 0.8, 0.1 and 1.7%, respectively. The least-squares analysis of variance demonstrated as follows; For the grasses, Ca content was significantly affected by the yearxseason, yearxaltitude, and the seasonxaltitude interactions. Mg content was significantly affected by year and season and P content was significantly affected by altitude, year and by the yearxaltitude interaction. For the legumes, Ca content was significantly affected by altitude and the yearxseason, seasonxaltitude and the yearxaltitude interactions and Mg content was significantly affected by season or altitude and by the yearxseason interaction. These results indicate that Ca content of forage plants grown at the lowland in rainy season was higher than at the highland in dry season. Mg content of forage plants grown at the lowland in dry season was higher than at the highland in rainy season and P content of forage plants grown in the highland was higher than in the lowland.
DOI： 10.5713/ajas.2004.63
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Nuritive evalution of forage plants grown in South Sulawesi, Indonesia 査読あり
Nasrullah, Niimi M., Akashi R., Kawamura O.
Asian-Australasian Journal of Animal Sciences 16 ( 5 ) 693 - 701 2003年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Asian-Australasian Journal of Animal Sciences
In order to evaluate the nutritive value of the forage plants in South Sulawesi, Indonesia, 266 samples (61 grasses and 65 legumes grown in the dry season, 60 grasses and 80 legumes grown in the rainy season) were collected from the highland and lowland in 1998 to 2000, and were subjected to the determination of chemical composition and digestibility. The least-squares analysis of variance demonstrated that the in vitro dry matter digestibility (IVDMD) of grasses was not significantly affected by season or altitude. On the other hand, the some proximate components and cell wall components were significantly affected by season and altitude including the seasonxaltitude interaction. For the legumes, the in vitro neutral detergent fiber digestibility (IVNDFD) and cellulose content were significantly affected by season. On the other hand, the ether extract (EE) content was significantly affected by season and altitude. The interaction of the seasonxaltitude for IVDMD, of the yearxseason for some proximate components and of the yearxseason and the seasonxaltitude for some cell wall components were significant. These results indicate that the forages grown at highland in dry season have a relatively high quality. The means of the total digestible nutrient (TDN) content estimated from IVDMD in grasses and in legumes were 50.3% and 57.4%, respectively, and the crude protein contents were 7.7% and 17.6%, respectively. The correlation coefficients between IVDMD and the contents of crude fiber, neutral detergent fiber and acid detergent fiber were relatively high in all of forage plants, suggesting that these components would provide an accurate prediction of digestibility or TDN content. A close relationship between IVNDFD and lignin content indicates that the lignin would be the most accurate predictor of cell wall digestibility.
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Nutritive evaluation of forage plants grown in South Sulawesi, Indonesia. 1. Chemical composition and in vitro digestibility.
共著者：ナスルラ，新美光弘，明石 良，川村修
Asian-Australasian Journal of Animal Sciences 16(5), 693-701, 2003年5月
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バヒアグラス（Paspalum notatum Flugge）における効率的な種子由来エンブリオジェニックカルス誘導法とパーティクルガンによる形質転換条件の検討
共著者：権藤崇祐，石井由紀子，明石 良，川村修
日本草地学会誌 49: 33-37 2003年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Super roots in Lotus corniculatus: a unique tissue culture and regeneration system in a legume species.
共著者：R. Akashi, T. Kawano, M. Hashiguchi, Y. Kutsuna, S.-S Hoffmann-Tsay,F. Hoffmann
Plant and Soil 255: 27-33 2003年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Variations in morphological and feeding characteristics in plants regenerated from embryogenic suspension cultures derived from a single genotype of bahiagrass (Paspalum notatum Flugge).
共著者：カマルディン・サレン，那須幹夫，新美光弘，明石 良，川村 修
Grassland Science,49(5): 456-459 2003年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）