論文 - 明石 良
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Ishigaki G., Gondo T., Suenaga K., Akashi R.
Grassland Science 55 ( 1 ) 46 - 51 2009年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Grassland Science
We have established a plant regeneration system via multiple shoot formation, or somatic embryogenesis from seed-derived shoot apical meristems of ruzigrass (Brachiaria ruziziensis). Shoot apices as initial explants were isolated from aseptically germinated seedlings, and cultured in vitro. The most effective treatments for multiple shoots and embryogenic calli formation were 0.5 mg L -1 2,4-dichlorophnoxyacetic acid (2,4-D) plus 2.0 mg L -1 6-benzylaminopurine (BAP) and 4.0 mg L -1 2,4-D plus 0.2 mg L -1 BAP, respectively. Plant regeneration from multiple shoot-clumps was achieved by culturing on solid MS medium containing 1.0 mg L -1 BAP or 2.0 mg L -1 kinetin plus 2.0 mg L -1 gibberellic acid. Somatic embryos regenerated plantlets on a MS medium without plant hormones. Regenerated shoots were germinated precociously on MS medium containing 0.01 or 0.1 mg L -1 naphthalene acetic acid and 2.0 mg L -1 BAP. All regenerants were successfully grown up to maturity. The plant regeneration via multiple-shoot clumps and embryogenic calli formation can be used as an alternative regenerable target tissue for genetic transformation using particle bombardment of ruzigrass. © Journal Compilation © 2009 Japanese Society of Grassland Science.
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Molecular characterization of a galactose-binding lectin from Momordica charantia seeds and its expression in tobacco cells. 査読あり
Tanaka H, Toyama J, Akashi R
Asian Journal of Plant Sciences 8 ( 8 ) 544 - 550 2009年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Establishment of multiple-shoot formation from apical meristems and dwarf strain induced by Heavy Iron Beam irradiation in cogongrass (Imperata cylindrical L.). 査読あり
Shigeki Y, Kutsuna Y, Yamamoto K, Isobe H, Abe T, Hashiguchi M, Akashi R
Jpn J Grassl Sci 55 227 - 232 2009年4月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Enhanced nodulation and nitrogen fixation in the abscisic acid low-sensitive mutant enhanced nitrogen fixation1 of Lotus japonicus 査読あり
Tominaga A, Nagata M, Futsuki K, Abe H, Uchiumi T, Abe M, Kucho KI, Hashiguchi M, Akashi R, Hirsch AM, Arima S, Suzuki A
Plant Physiology 151 ( 4 ) 1965 - 1976 2009年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Gondo T., Matsumoto J., Tsuruta S., Yoshida M., Kawakami A., Terami F., Ebina M., Yamada T., Akashi R.
Journal of Plant Physiology 166 ( 4 ) 435 - 441 2009年3月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Plant Physiology
Rhodes grass (Chloris gayana) is one of the most important warm-season forage grasses. It is cultivated in tropical and subtropical parts of the world and is mostly used for grazing and hay production. We have established a particle-bombardment transformation protocol for rhodes grass using multiple-shoot clumps (MSCs) as the target tissue. A vector pAHC25 containing a herbicide-resistance gene (bar) together with the β-glucuronidase (GUS) gene was used in transformation experiments. The most efficient recovery of bialaphos-resistant tissue was achieved when the bombarded MSCs were first cultured for 15 d on bialaphos-free medium before being subjected to selection pressure. The resistant tissues regenerated transgenic plants that displayed GUS gene expression. Under optimized conditions, 251 target pieces yielded 46 transgenic plants from 4 independent transgenic lines. © 2008 Elsevier GmbH. All rights reserved.
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Particle inflow gun-mediated transformation of multiple-shoot clumps in rhodesgrass (Chloris gayana). 査読あり
Gondo T, Matsumoto J, Tsuruta S, Yoshida M, Kawakami A, Terami F, Ebina M, Yamada T,
Journal of Plant Physiology 166 435 - 441 2009年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Immunoadjuvant activity of crude lectin extracted from Momordica charantia seed 査読あり
Li Huang, Ai Ikejiri, Yuya Shimizu, Takumi Adachi, Yoshitaka Goto, Jun Toyama, Hidenori Tanaka, Ryo Akashi, Kazuyuki Uchida, Hironori Miyata, Takeshi Haga
J. Vet. Med. Sci. 70 ( 5 ) 533 - 535 2009年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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チガヤ(Imperata cylindrica L.)における多芽体誘導と重イオンビーム照射による矮性系統の選抜 査読あり
繁木 雄一, 久綱 泰代, 山本 耕造, 磯部 弘, 阿部 知子, 橋口 正嗣, 明石 良
日本草地学会誌 55 ( 3 ) 227 - 232 2009年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本草地学会
チガヤの生長点から多芽体カルスを誘導し,効率的な植物体再分化系を確立するとともに,この多芽体カルスを用いて重イオンビームを照射し,矮性系統の作出を試みた。葉原基を1-2枚残した生長点は,MS培地に,2,4-DおよびBAPをそれぞれ組み合わせて添加し培養を行った。その結果,0.1mg/L 2,4-Dおよび2.0mg/L BAPの添加で最も高い形成率を示した。その後,この多芽体カルスは再分化培地でシュートを形成させ,発根培地で発根を促すことで,多くの植物体を得ることができた。チガヤの生長点由来多芽体カルスは,重イオンビーム(ネオンイオンおよび炭素イオン)を照射したところ,3年間の育成期間と形態的特性調査により,ネオンイオン20Gy照射区から草丈の極めて低い矮性系統が見いだされた。
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Characterization of lectin isolated from Momordica charantia seed as a B cell activator 査読あり
Huang L., Adachi T., Shimizu Y., Goto Y., Toyama J., Tanaka H., Akashi R., Sawaguchi A., Iwata H., Haga T.
Immunology Letters 121 ( 2 ) 148 - 156 2008年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Immunology Letters
Lectin isolated from the seeds of Momordica charantia (MCL) is a galactose-specific glycoprotein. To investigate the effects of MCL on cell activation, we analyzed the responses of BALB/c splenocytes, thymocytes, T cells and B cells on MCL stimulation. Proliferation assays showed that MCL selectively stimulates the B cell subset of splenocytes (p < 0.05) in a dose and time dependent manner and that this activation proceeds without the involvement of T cells. Flow cytometric analysis revealed that the fluorescein isothiocyanate (FITC)-labeled MCL binds to B cells, which was inhibited by specific sugars, including galactose. Mouse immunoglobulin (Ig) was able to inhibit MCL-induced proliferation of mouse B cells, suggesting MCL stimulates B cell activation via membrane Ig in the B cell surface. Moreover, after 96-h co-culture, MCL triggered splenocytes to produce a large amount of non-specific IgM in culture supernatants (p < 0.01). Additionally, MCL was shown to up-regulate the cell activation marker CD86, in a B cell subpopulation distinct from that affected by LPS. These data suggest that MCL is a T cell-independent B cell activator and a polyclonal Ig inducer, and provide further information on the immunomodulatory effect of MCL. © 2008 Elsevier B.V. All rights reserved.
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Multiple shoot formation, somatic embryogenesis and plant regeneration from seed-derived shoot apical meristems in ruzigrass (Brachiaria ruziziensis). 査読あり
Ishigaki G, Gondo T, Suenaga K, Akashi R
Grassland Science 55 2008年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sequencing and analysis of approximately 40,000 soybean cDNA clones from a full-length enriched cDNA library. 査読あり
Umezawa T, Sakurai T, Totoki Y, Toyoda A, Seki M, Ishiwata A, Akiyama K, Kurotani A, Yoshida T, Mochida K, Kasuga M, Todaka D, Maruyama K, Nakashima K, Enju A, Mizukado S, Ahmed S, Yoshiwara K, Harada K, Tsubokura Y, Hayashi M, Sato S, Anai T, Ishimoto M, Funatsuki H, Teraishi M, Osaki M, Shinano T, Akashi R, Sakaki Y, Yamaguchi-Shinozaki K, Shinozaki K
DNA Research 2008年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Purification and characterization of Anti-H lectin from the seed of Momordica charantia and inter-specific differences of hemagglutinating activity in Cucurbitaceae 査読あり
Jun Toyama, Hidenori Tanaka, Akiko Horie, Tetsuya Uchiyama, Ryo Akashi
Asian Journal of Plant Sciences 7 ( 7 ) 647 - 653 2008年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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'Mimi' sweet pea for forcing culture 査読あり
Kaoru Nakamura, Hirotoshi Hino, Sadao Gunji, Norio Hattanda, Toshio Murata, Hiroshi Tominaga, Koichi Fukumoto, Ryo Akashi
HortScience 43 ( 7 ) 2238 - 2239 2008年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Toyama J., Tanaka H., Horie A., Uchiyama T., Akashi R.
Asian Journal of Plant Sciences 7 ( 7 ) 647 - 653 2008年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Asian Journal of Plant Sciences
Thirteen Cucurbitaceae species have been investigated for anti-H activity of seed lectins. The lectin was extracted from seed powder and concentrated by ethanol precipitation method. Momordica charantia, Trichosanthes kirilowii, T. cucumeroides and T. bracteata had potent hemagglutinating (HA) activity toward human type-H(O) erythrocytes, in which M. charantia exhibited considerably lower activity toward human type-O m h (para-Bombay, H-deficient). Hence, it was characterized as anti-H activity. Eight Japanese cultivars exhibited almost same degrees of anti-H activity. A lectin from seeds of M. charantia has been purified by affinity chromatography and gel-permeation. The lectin was shown to be a glycoprotein containing approximately 10% neutral sugar, which gave a single band on native polyacrylamide gel electrophoresis (PAGE) and four bands of 31.5, 30.5, 30.0 and 28.5 kDa on SDS-PAGE under reducing conditions suggesting that the lectin is a tetramer. The HA activity was stable at 50°C for 1 h, but sharply decreased beyond 55°C. The lectin agglutinated human type-O erythrocytes and the agglutination was inhibited by D-galactose and its derivatives, particularly human blood type-H (O) antigen trisaccharide (Fucα1→2Galβ1→4GlcNAc). These results suggest that M. charantia seed lectin has anti-H (O) activity and D-galactose specificity. Inter-specific differences in anti-H activity of the seed among Cucurbitaceae may exist. © 2008 Asian Network for Scientific Information.
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Umezawa T., Sakurai T., Totoki Y., Toyoda A., Seki M., Ishiwata A., Akiyama K., Kurotani A., Yoshida T., Mochida K., Kasuga M., Todaka D., Maruyama K., Nakashima K., Enju A., Mizukado S., Ahmed S., Yoshiwara K., Harada K., Tsubokura Y., Hayashi M., Sato S., Anai T., Ishimoto M., Funatsuki H., Teraishi M., Osaki M., Shinano T., Akashi R., Sakaki Y., Yamaguchi-Shinozaki K., Shinozaki K.
DNA Research 15 ( 6 ) 333 - 346 2008年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：DNA Research
A large collection of full-length cDNAs is essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We obtained a total of 39 936 soybean cDNA clones (GMFL01 and GMFL02 clone sets) in a full-length-enriched cDNA library which was constructed from soybean plants that were grown under various developmental and environmental conditions. Sequencing from 5′ and 3′ ends of the clones generated 68 661 expressed sequence tags (ESTs). The EST sequences were clustered into 22 674 scaffolds involving 2580 full-length sequences. In addition, we sequenced 4712 full-length cDNAs. After removing overlaps, we obtained 6570 new full-length sequences of soybean cDNAs so far. Our data indicated that 87.7 of the soybean cDNA clones contain complete coding sequences in addition to 5′- and 3′-untranslated regions. All of the obtained data confirmed that our collection of soybean full-length cDNAs covers a wide variety of genes. Comparative analysis between the derived sequences from soybean and Arabidopsis, rice or other legumes data revealed that some specific genes were involved in our collection and a large part of them could be annotated to unknown functions. A large set of soybean full-length cDNA clones reported in this study will serve as a useful resource for gene discovery from soybean and will also aid a precise annotation of the soybean genome.
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Genetic diversity of chloroplast DNA in Zoysia and other warm-season turfgrasses 査読あり
Tsuruta Shin-ichi, Hosaka Fumiko, Otabara Tesshu, HASHIGUCHI Masatsugu, YAMAMOTO Toshiya, AKASHI Ryo
Grassland science 54 ( 3 ) 151 - 159 2008年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japanese Society of Grassland Science
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Genetic diversity of chloroplast DNA in Zoysia and other warm-season turfgrasses. 査読あり
Tsuruta S, Hosaka F., Otabara T., Hashiguchi M., Yamamoto T., Akashi R
Grassland Science 54 151 - 159 2008年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Transgenic superroots of Lotus corniculatus can be regenerated from superroot-derived leaves following Agrobacterium-derived transformation. 査読あり
Tanaka H, Toyama J, Hashiguchi M, Kutsuna Y, Tsuruta S, Akashi R, Hoffmann F
Journal of Plant Physiology 165 1313 - 1316 2008年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Tanaka H., Toyama J., Hashiguchi M., Kutsuna Y., Tsuruta S., Akashi R., Hoffmann F.
Journal of Plant Physiology 165 ( 12 ) 1313 - 1316 2008年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Plant Physiology
Super-growing roots (superroots; SR), which have been established in the legume species Lotus corniculatus, are a fast-growing root culture that allows continuous root cloning, direct somatic embryogenesis and mass regeneration of plants under entirely growth regulator-free culture conditions. These features are unique for non-hairy root cultures, and they are now stably expressed since the culture was isolated more than 10 years ago (1997). Attempts to achieve direct and stable transformation of SR turned out to be unsuccessful. Making use of the supple regeneration plasticity of SR, we are reporting here an indirect transformation protocol. Leaf explants, derived from plants regenerated from SR, were inoculated with Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pBI121, which contains the neomycin phosphotransferase II (NPTII) and β-glucuronidase (GUS) genes as selectable and visual markers, respectively. After co-cultivation, the explants were selected on solidified MS medium with 0.5 mg/L benzylamino purine (BAP), 100 mg/L kanamycin and 250 mg/L cefotaxime. Kanamycin-resistant calli were transferred to liquid rooting medium. The newly regenerated, kanamycin-resistant roots were harvested and SR cultures re-established, which exhibited all the characteristics of the original SR. Furthermore, kanamycin-resistant roots cultured onto solidified MS medium supplemented with 0.5 mg/L BAP produced plants at the same rate as control SR. Six months after gene transfer, PCR analysis and histochemical locating indicated that the NPTII gene was integrated into the genome and that the GUS gene was regularly expressed in leaves, roots and nodules, respectively. The protocol makes it now possible to produce transformed SR and nodules as well as transgenic plants from transformed SR. © 2008 Elsevier GmbH. All rights reserved.
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ニガウリ種子由来粗精製レクチンの免疫アジュバント効果 査読あり
黄 莉, 池尻 藍, 清水 佑也, 足立 匠, 後藤 義孝, 外山 潤, 田中 秀典, 明石 良, 内田 和幸, 宮田 博規, 芳賀 猛
The journal of veterinary medical science 70 ( 5 ) 533 - 535 2008年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：社団法人日本獣医学会
ニガウリ種子由来粗精製レクチン（crMCL）のアジュバント効果について、ベータガラクトシダーゼをモデル抗原として解析した。BALB／cマウスを抗原単独投与群と抗原＋crMCL投与群に分け、血清中の抗原特異的IgG抗体価を測定したところ、1回免疫では両群で差がなかったが、追加免疫で抗原＋crMCL投与群が有意に高かった。crMCLはプライムブースト投与法に有用なアジュバントであることが示唆された。