論文 - 國武 久登
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Yahata Masaki, Nukaya Tsunaki, Sudo Miki, Ohta Tomohiro, Yasuda Kiichi, Inagaki Hidehiro, Mukai Hiroo, Harada HIsashi, Takagi Toshihiro, Komatsu Haruki, Kunitake Hisato
The Horticulture Journal 84 ( 1 ) 30 - 36 2015年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.2503/hortj.MI-005
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Yano Tsubasa, Tanaka Hidenori, Kurino Taiki, Yamamoto Akihiro, Kunitake Hisato, Saeki Yuichi, Akashi Ryo
Plant Root 9 6 - 14 2015年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Abe A., Uchida A., Hoshino Y., Sakakibara Y., Suiko M., Kunitake H.
Acta Horticulturae 1065 1267 - 1274 2015年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Acta Horticulturae
To analyze the protein expression of pollen grains and tubes having self-incompatible reactions in Hyuganatsu (Citrus tamurana Hort. ex Tanaka), we cultured mature pollen and treated it with crude extracts from the styles using a mass or single liquid culture system, and we collected pollen grains and tubes for proteome analysis. The relative expression of each identified protein was quantified by an imager and revealed as a value relative to the pollen grain. Eleven identified proteins were remarkably up-regulated (above 1.2-fold) or down-regulated (under 0.8-fold) in treatment with self-incompatible reactions. Eight of these proteins were predicted to be self-incompatible-related proteins with the reported functions. In this study, F-box protein was identified as showing minimum expression in the treatment with crude extracts from styles of Hyuganatsu (self-incompatible reactions). The S-locus gene product expressed in pollen has been identified as an F-box protein in Solanaceae and Rosaceae. It is not clear whether a mechanism similar to that of Solanaceae and Rosaceae exists in the self-incompatible reaction in Hyuganatsu; however, the reduced expression of F-box protein may induce a self-incompatible reaction.
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Yasuda Kiichi, Yahata Masaki, Kunitake Hisato
The Horticulture Journal 0 ( 0 ) 2015年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：The Japanese Society for Horticultural Science
Kumquats (<i>Fortunella</i> spp.) is classified into the subfamily Aurantioideae (family Rutaceae). The taxonomy and phylogeny of this genus are complicated and controversial. Therefore, we carried out a estimation based on chromomycin A<sub>3</sub> (CMA) karyotype composition in order to understand the cytogenetics and evolution of the genus <i>Fortunella</i>. Among the 6 <i>Fortunella</i> species examined, <i>Fortunella hindsii</i> var. <i>chintou</i> Swing. (Hongkong kumquat) showed the simplest CMA karyotype composition. On the other hand, close relationships were found among the 3 species <i>F. margarita</i> (Lour.) Swing. (Oval kumquat), <i>F. japonica</i> (Thunb.) Swing. (Round kumquat), <i>F. crassifolia</i> Swing. (Meiwa kumquat). <i>Fortunella polyandra</i> (Ridl.) Tan. (Malayan kumquat) and <i>F. obovata</i> hort. ex Tan. (Changshou kumquat) had type E chromosomes, which is an elemental chromosome type for <i>Citrus</i>. We concluded that there are only two true species for the genus <i>Fortunella</i>, <i>F. hindsii</i> and <i>F. margarita</i> complex, which includes <i>F. margarita</i>, <i>F. japonica</i>, and <i>F. crassifolia</i>, and that <i>F. polyandra</i> and <i>F. obovata</i> should be classified as natural or horticultural hybrids.
DOI： 10.2503/hortj.MI-078
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Li Y., Uchida A., Abe A., Yamamoto A., Hirano T., Kunitake H.
Journal of the American Society for Horticultural Science 140 ( 2 ) 183 - 190 2015年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of the American Society for Horticultural Science
© 2015, American Society for Horticultural Science. All rights reserved. Citrus is a genus with gametophytic self-incompatibility, which prevents fertilization by self-pollination. Polyamines (PAs), as a class of active small molecules, widely participate in various cellular activities. To investigate the effects of the changes in PA contents [putrescine, spermidine (SPD), and spermine] on pollen tube growth in self-incompatible (SI) plants, we cultured pollen of Citrus maxima ‘Banpeiyu’ and C. tamurana ‘Hyuganatsu’ in a Citrus mature pollen culture system and detected the PA content changes in pollen by high-performance liquid chromatography (HPLC) analysis during germination and after treatment with crude protein extracts of compatible or incompatible styles. We profiled the PA content changes during the germination course in pollen of ‘Banpeiyu’ and ‘Hyuganatsu’ by HPLC, and identified increases of free and perchloric-acid-insoluble SPD contents in pollen tubes in the incompatible treatment. Exogenous SPD treatment obviously increased the SPD contents in the pollen tube, inhibited pollen tube elongation, and resulted in some abnormal morphological alterations, such as tip inflation, tube content leakage and tip twist, which were observed in both ‘Banpeiyu’ and ‘Hyuganatsu’ pollen tubes exposed to incompatible treatment. This finding implied that SPD might participate in the SI response in Citrus.
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クロマメノキとラビットアイブルーベリーT100との節間交雑から得られたF1系統の評価 査読あり
執行 みさと、森田 恭代、西村 謙一、井上 論司、國武 久登、小松 春喜
園芸学研究 13 ( 4 ) 323 - 333 2014年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
DOI： 10.2503/hrj.13.323
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The phenomenon of root elongation and high respiration actibity in the rolB-gene-enhanced FSL#35 variant of Lotus corniculatus FOX-SR line. 査読あり
Yano Tsubasa, Yamamoto Akihiro, Kunitake Hisato, Saeki Yuichi, Akashi Ryo
Plant Root 8 82 - 91 2014年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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我が国自生スノキ属野生種クロマメノキとハイブッシュブルーベリー‘ブルークロップ’との節間交雑から得られたF1系統の評価 査読あり
執行みさと、具志堅 文、桂川明広、臂 光昭、吉岡克則、鹿毛哲郎、國武久登、小松春喜
園芸学研究 13 ( 2 ) 97 - 106 2014年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Comparison of Cultivars and Seasonal Variation in Blueberry (<i>Vaccinium</i> Species) Leaf Extract on Adult T-Cell Leukemia Cell Line Growth Suppression.
Kai H, Fuse T, Kunitake H, Morishita K, Matsuno K
Medicines (Basel, Switzerland) 1 ( 1 ) 3 - 11 2014年6月
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我が国自生スノキ属野生種クロマメノキとハイブッシュブルーベリー ブルークロップとの節間交雑から得られたF1系統の評価 査読あり
執行 みさと、具志堅 文、桂川 明広、臂 光昭、吉岡 克則、鹿毛 哲朗、國武 久登、小松 春喜
園芸学研究 13 ( 2 ) 97 - 106 2014年4月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
DOI： 10.2503/hrj.13.97
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ブルーベリー近縁種ナツハゼとアラゲナツハゼにおける果実の成熟特性と品質評価 査読あり
津田浩利, 小島祥子, 大坪早貴, 小松春喜, 國武久登
園芸学研究 13 ( 1 ) 1 - 9 2014年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Efficient in vitro screening for higher soil pH adaptability of intersectional hybrids in blueberry
Tsuda H., Kunitake H., Aoki Y., Oyama A., Tetsumura T., Komatsu H., Yoshioka K.
HortScience 49 ( 2 ) 141 - 144 2014年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：HortScience
We tested efficient in vitro methods for screening the genotypes with higher pH tolerance using multiple shoots of intersectional hybrids between Vaccinium corymbosum 'Spartan' and V. bracteatum. The response of the four hybrid clones tested to different pH levels was clone-dependent in vitro. An apparent difference was found in the rooting rate among the hybrid clones even at higher pH levels; the rooting rates of JM4 (91%) at pH 8.0 indicated a significantly high value compared with other clones (JM1: 24%, JM2: 9%, JM3: 8%, 'Spartan': 0%). Furthermore, JM4 showed constantly high rooting rates (91% to 100%) at all pH levels with no significant differences. Similar differences in the root characters of the hybrids were also confirmed by checking the viability of roots using fluorescein diacetate (FDA)/propidium iodide (PI) staining after dipping the roots of in vitro-produced shoots in liquid medium at different pH levels for 6 hours. These results suggest that an in vitro screening method using the rooting rate of multiple shoots and the viability test of roots by FDA/PI staining as a marker could become a very useful tool for the selection of germplasm with tolerance to higher pH within a short time using small planting spaces. In addition, JM4, which showed a high rooting rate at pH 8.0, could be useful in breeding new cultivars with higher pH tolerance.
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ブルーベリー近縁種ナツハゼとアラゲナツハゼにおける果実の成熟特性と品質評価
津田 浩利, 小島 祥子, 大坪 早貴, 小松 春喜, 國武 久登
園芸学研究 13 ( 1 ) 1 - 9 2014年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：園芸学会
我が国に自生するブルーベリー近縁種ナツハゼとアラゲナツハゼをブルーベリーの育種へ利用するための基礎的情報を得ることを目的として，果実の成熟特性調査および果実成分の評価を行った．ナツハゼとアラゲナツハゼは，栽培品種と比較して1果房当たりの果実数が多く細長い穂状の果房を着生した．また，同一果房内のすべての果実が成熟するまで樹上に着生させても，最初に成熟した果実は落果しないため，ブドウのように房取り収穫ができる可能性が示唆された．さらに，果実の成熟期は9～11月となり，ブルーベリーの極晩生品種より1～3か月遅いことが明らかとなった．果実の成分を分析した結果，ナツハゼとアラゲナツハゼ成熟果の糖酸比はブルーベリーと比較して低く食味は劣ったが，ナツハゼ成熟果の総アントシアニン含量はブルーベリーの約6倍であった．総アントシアニン含量はすべての材料において成熟果で最も高い値を示した．一方，総ポリフェノール含量，総プロアントシアニジン含量および抗酸化能の変化を調査したところ，ブルーベリーではいずれも未熟果で最も高い値を示し成熟が進むにつれて減少したが，ナツハゼとアラゲナツハゼでは成熟に伴い再び増加した．また，アラゲナツハゼ成熟果の総ポリフェノール含量は栽培品種の約3倍，総プロアントシアニジン含量では約17倍となった．さらに，供試材料で最も高い値を示したアラゲナツハゼの抗酸化能には，プロアントシアニジンを含むポリフェノールが寄与している可能性が示唆された．以上のように，ナツハゼとアラゲナツハゼは，ブルーベリーの収穫期間を拡大し，房取り収穫可能な高い機能性を有する品種育成のための育種素材として有望と考えられた．
DOI： 10.2503/hrj.13.1
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執行みさと、竹馬昂平、國武久登、小松春喜
園芸学研究 12 ( 4 ) 335 - 342 2013年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Tsuda H., Kunitake H., Yamasaki M., Komatsu H., Yoshioka K.
Journal of the American Society for Horticultural Science 138 ( 4 ) 317 - 324 2013年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of the American Society for Horticultural Science
With crosses between colchicine-induced tetraploid shashanbo (Vacciniumbracteatumsection Bracteata) and tetraploid highbush blueberry 'Spartan' (Vaccinium corymbosum section Cyanococcus), intersectional hybrids were produced. The hybridity of these plants was confirmed based on DNA markers and morphological characteristics. The morphological characteristics, blooming date, and ripening period of the hybridswere intermediate between those of the parents. Ploidy analyses by flow cytometry and chromosome counting revealed that these hybrids were tetraploid. Four hybrids set fruit in the field and these two hybrids showed high pollen stainability. It was noteworthy that fruit of two hybrids had high soluble solids concentration compared with 'Spartan' and the fruit pulp of the hybrids was tinged with red as shashanbo. These hybrids could be useful in breeding new cultivars with high sugar content, abundant phytochemicals, extensive environmental adaptability as well as late flowering and fruit maturity.
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Tsuda, H., Kunitake, H., Ryoko Kawasaki-Takaki, R., Nishiyama, K., Yamasaki, M., Komatsu, H., Yukizaki
Plants 2 ( 1 ) 57 - 71 2013年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Antioxidant Activities and Anti-Cancer Cell Proliferation Properties of Natsuhaze (Vaccinium oldhamii Miq.), Shashanbo (V. bracteatum Thunb.) and Blueberry Cultivars.
Tsuda H, Kunitake H, Kawasaki-Takaki R, Nishiyama K, Yamasaki M, Komatsu H, Yukizaki C
Plants (Basel, Switzerland) 2 ( 1 ) 57 - 71 2013年2月
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執行 みさと, 竹馬 昂平, 國武 久登, 小松 春喜
園芸学研究 12 ( 4 ) 335 - 342 2013年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：園芸学会
キイチゴ（<i>Rubus</i> spp.）は，果実中に含有されるフェノール化合物が健康維持に効果的と考えられることから注目されつつある果樹である．我が国にも多くの野生種が自生しているが，栽培されている品種のほとんどは欧米で改良されたものである．本研究では，野生種を園芸的に利用するための基礎的な知見を得るため，九州地方に自生するいくつかの野生種を収集し，育種素材としての評価を行った．栽培種はいずれも二倍体であったが，野生種はゲノムサイズの異なる二倍体と少数の六倍体であった．シマバライチゴを除く野生種の集合果は，栽培種と比較して小さくなかった．野生種の中にはクマイチゴ，カジイチゴおよびナガバモミジイチゴのように栽培種に比べ果実中の糖含量が高く，有機酸含量が低いものがあった．また，クマイチゴ，ナガバモミジイチゴおよびシマバライチゴは栽培種に比べ果実中の総ポリフェノール含量あるいは抗酸化活性が高かった．我が国自生の野生種のいくつかは，栽培し易く，果実品質が優れかつ機能性の高い品種を育成するための育種素材となり得るものと思われた．<br>
DOI： 10.2503/hrj.12.335
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Effective micropropagation of rabbiteye blueberries for leaf tea production
Tetsumura T., Kajiwara Y., Honsho C., Sato-Yamauchi M., Sugimoto Y., Kunitake H.
Environmental Control in Biology 50 ( 3 ) 289 - 296 2012年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Environmental Control in Biology
Micropropagation of two clones of rabbiteye blueberry (Vaccinium virgatum Aiton) developed for leaf tea production was investigated for supply in high quantities to plantations and plant factories. Shoots of a new cultivar, 'Kunisato 35 Gou', were multiplied efficiently on MW, a mixture of equal parts of MS and WPM, supplemented with 20 μM zeatin by using 2-node segments. For No. 19, another promising clone, 5-node segments were the most productive for shoot multiplication. Among basal media tested for micropropagation, MW medium was found to be the most efficient. Although shoots of 'Kunisato 35 Gou' grew better in vitro than those of No. 19, both clones had equivalent rooting abilities. More than 90% of the shoots rooted and survived in vermiculite after culturing in Gellan gum-solidified rooting medium without plant growth regulators. The growth of shoots with roots in pots was significantly influenced by the potting soil. The plantlets in the soils mixed with peat moss, which made the soil acid, grew vigorously, while those in the soils without peat moss grew moderately or poorly, depending on soil type.
DOI： 10.2525/ecb.50.289
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Identification of self-incompatibility related proteins in the pistil of Japanese pear (Pyrus pyrifolia (Burm.f.)) by proteome analysis. 査読あり
1 Uchida, U., H. Sassa, S. Takenaka, Y. Sakakibara, M. Suiko, H. Kunitake
Plant Omics Journal ５ ( ４ ) 320 - 325 2012年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）