論文 - 平野 智也
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Vuong Quoc Nhat, Yusuke Kazama, Kotaro Ishii, Sumie Ohbu, Hisato Kunitake, Tomoko Abe, Tomonari Hirano
Plants 10 ( 9 ) 1881 2021年9月
担当区分：最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plants
Two growth processes, cell proliferation and expansion, determine plant species-specific organ sizes. A large flower mutant in Arabidopsis thaliana, ohbana1 (ohb1), was isolated from a mutant library. In the ohb1 flowers, post-mitotic cell expansion and endoreduplication of nuclear DNA were promoted. The whole-genome resequencing and genetic analysis results showed that the loss of function in MEDIATOR16 (MED16), a mediator complex subunit, was responsible for the large flower phenotypes exhibited by ohb1. A phenotypic analysis of the mutant alleles in MED16 and the double mutants created by crossing ohb1 with representative large flower mutants revealed that MED16 and MED25 share part of the negative petal size regulatory pathways. Furthermore, the double mutant analyses suggested that there were genetically independent pathways leading to cell size restrictions in the floral organs which were not related to the MED complex. Several double mutants also formed larger and heavier seeds than the wild type and single mutant plants, which indicated that MED16 was involved in seed size regulation. This study has revealed part of the size-regulatory network in flowers and seeds through analysis of the ohb1 mutant, and that the size-regulation pathways are partially different between floral organs and seeds.
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Toshima S., Hirano T., Kunitake H.
Scientia Horticulturae 285 110204 2021年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Scientia Horticulturae
The breeding of raspberries (Rubus idaeus L.) and blackberries (R. fruticosus Agg.) has shifted in focus toward traits associated with the sensory qualities and potential health benefits of the fruit, and Rubus wild species have attracted a great deal of attention as new sources of genetic diversity. In this study, the anthocyanin and polyphenol contents and compositions, and three types of antioxidant capacities were compared in mature fruits including raspberries, blackberries, and Japanese wild Rubus species. ‘Blackcap’ black raspberry showed the significantly highest values of total anthocyanin content and total polyphenol content among 12 Rubus species or cultivars. Cyanidin-3-xylosyl-rutinoside was contained only in black raspberries, and was characteristic anthocyanin in Rubus species. R. croceacanthus H. Lév., a Japanese wild species that is adapted to warm regions, had a deep red fruit color, and had the highest total anthocyanin and total polyphenol contents among the four Japanese wild species examined. As for the antioxidant capacities, the highest values were observed in ‘Blackcap’ among 12 Rubus species. Furthermore, a high positive correlation was shown between total anthocyanin or total polyphenol content and antioxidant capacities. In particular, black raspberries and blackberries contained high concentrations of rutin. Thus, rutin might contribute to the high antioxidant capacities in these cultivars.
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Changes in polyphenols, anthocyanins, and DPPH radical-scavenging activities in sweetpotato (Ipomoea batatas L.) during tuber growth 査読あり
Soya Nakagawa, Ryo Ohmura, Saki Toshima, HyungjunPark, Yosuke Narasako, Tomonari Hirano, Motoyasu Otani, Hisato Kunitake
Scientia Horticulturae 284 110100 2021年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Kawano M., Yahata M., Shimizu T., Honsho C., Hirano T., Kunitake H.
Scientia Horticulturae 277 109782 2021年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Scientia Horticulturae
The genetic analysis of five haploid plants (BX1-BX5) of ‘Banpeiyu’ pummelo (BP), [Citrus maxima (Burm.) Merr.] using 32 simple sequence repeat (SSR) markers carried out for the purpose of confirming their gynogenetic origin and genetic differences. As the results, all the haploid plants possessed only either one of the BP alleles, confirming that they are gynogenetic origin. We also confirmed that BX1 and its doubled haploid (BX1-DH) have exactly the same SSR alleles. Interestingly, all the five haploids had common alleles on the linkage group (LG) 6 in the case of three markers. Then, we performed self-pollination of DH plants and reciprocal crosses between DH and BP, which has strong self-incompatibility. The seeds obtained by these crosses germinated in vitro or ex vitro normally and developed into diploid seedlings, except for one tetraploid seedling. Genetic analysis of these seedlings with 32 SSR markers revealed that segregation ratios of the 32 loci in the 30 BP inbred, 30 BP x DH and 30 DH x BP progenies mostly fitted to the expected ratios. However, significantly distorted segregation ratios were found in one, five and three SSR markers for BP inbred, BP x DH and DH x BP progenies, respectively. All of the 30 DH inbred progenies showed exactly the same SSR alleles as the original DH. This is the first example in which self and back cross progenies of DH in fruit crops were genetically analyzed using SSR markers.
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Breeding of Summer-Autumn-flowering Small-flowered Spray-type Chrysanthemums cultivar ‘Nagasaki SWC1’ by heavy-ion beam irradiation 査読あり
A. Hisamura, J. Morooka, T. Abe, Y. Hayashi, T. Hirano
RIKEN Accelerator Progress Report 2019 53 204 2020年10月
記述言語：英語 掲載種別：研究論文（大学，研究機関等紀要）
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Mutant induction in gametophytes of Undaria pinnatifida (Phaeophyceae) by heavy ion beam irradiation 査読あり
Hirano T., Sato Y., Ichinose K., Yamada M., Hayashi Y., Fukunishi N., Abe T.
Phycological Research 68 ( 1 ) 63 - 69 2020年1月
担当区分：筆頭著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Phycological Research
© 2019 Japanese Society of Phycology Undaria pinnatifida, the brown macroalga, is a major commercial edible seaweed, and there is increasing interest in breeding new, improved cultivars for market expansion. In this study, we attempted to establish mutagenesis in U. pinnatifida using Ar and C ion beams as mutagens to meet future demands. To screen irradiated generations for mutants, U. pinnatifida zoospores irradiated with Ar and C ion beams were cultivated in plastic Petri dishes. Some gametophytes derived from the irradiated zoospores showed growth arrest or cell death at the initial developmental stage. Although the growth inhibition and lethal effects were observed at high doses of each ion irradiation, the Ar ion irradiation had high biological effects on cell division and growth. The gametophytes that showed a reduction in cell elongation were designated as an inhibited cell elongation mutant. A comparison of the mutant induction frequencies revealed that the C ion beam showed a higher frequency than the Ar ion beam. The highest frequency was 0.83% at 12.5 Gy of the C ion beam. We determined the total number of sporophytes and embryos per female gametophyte after sporophyte induction. High-dose irradiation with the Ar ion beam decreased the embryo and sporophyte formation, suggesting that the Ar ion beam also has exhibited high biological effects on the fertilization or embryogenesis processes or both. The developed heavy ion mutagenesis and mutant screening methods would be useful for mutation breeding and constructing specific mutant libraries in brown algae, and not only in U. pinnatifida.
DOI： 10.1111/pre.12397
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Death of pollen tetrads caused by chromosomal rearrangement 査読あり
Y. Kazama, H. Abe, K. Ishii, M. Sato, T. Hirano, K. Toyooka, T. Abe
RIKEN Accelerator Progress Report 2018 52 215 2019年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effects of carbon-ion irradiation to male gametes on double fertilization in Cyrtanthus mackenii 査読あり
T. Hirano, Y. Watarikawa, Y. Hayashi, T. Abe, H. Kunitake
RIKEN Accelerator Progress Report 2018 52 214 2019年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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New ornamental cherry cultivars induced by heavy-ion beam irradiation 査読あり
Hayashi Y., Ishii S., Hirano T., Ichinose K., Kazama Y., Abe T.
Acta Horticulturae 1235 99 - 104 2019年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Acta Horticulturae
© 2019 International Society for Horticultural Science. All rights reserved. We have developed a unique technology for mutation induction using heavy-ion beams. The irradiation treatment is quick, lasting between a few seconds and a few minutes, and is sufficient to induce mutation. Some new cherry blossom cultivars have already been introduced to the market. Pale-yellow cherry blossom 'Nishina Zao' was created by irradiating greenish 'Gyoiko' (Prunus lannesiana) scions with carbon ions. Unlike normal cherry blossom trees (P. pauciflora 'Keio-zakura Yamagata No.13'), 'Nishina Otome' does not require a certain period under cold winter weather to induce flowering in the spring. Both cultivars are propagated by cloning branches of mutant plants. We have also succeeded in creating new cultivars using progeny of irradiated plants that are not changed with respect to either flower color or shape. Although the original cultivar, P lannesiana 'Beni-yutaka', has a double flower with 15-20 petals, the new cultivar 'Nishina Tomoka' has a large, single flower with 5 petals. Heavy-ion beams are thus effective tools for creating new variations of Prunus trees through mutation breeding.
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LET dependence on killing effect and mutagenicity in the model filamentous fungus Neurospora crassa. 査読あり
Ma L, Kazama Y, Hirano T, Morita R, Tanaka S, Abe T, Hatakeyama S
International journal of radiation biology 94 ( 12 ) 1125 - 1133 2018年10月
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Analysis of DNA damage response in Cyrtanthus pollen after Ar-ion beam irradiation 査読あり
T. Hirano, A. Hanada, Y. Hayashi, T. Abe, H. Kunitake
RIKEN Accelerator Progress Report 51 240 2018年10月
記述言語：英語 掲載種別：研究論文（大学，研究機関等紀要）
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Kazama Y., Hirano T., Abe T., Matsunaga S.
Cytologia 83 ( 2 ) 125 - 128 2018年6月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cytologia
Chromosomal rearrangements are thought to play a role in speciation, adaptation and evolution. The effects of chromosomal rearrangements on gene expression have been reported in many species and their close relatives, and may illustrate the consequences of adaptation. To investigate the effects of chromosomal rearrangements on phenotypic changes directly, studies of experimentally induced rearrangements are required; they allow gene expression and epigenetic states to be compared between mutants and wild-types. We have developed an effective method for inducing chromosomal rearrangements with heavy-ion beam irradiation, which is a sort of ionizing radiation. Reconstruction of such rearrangements by genome editing will enable reproducible studies on the effects of chromosomal rearrangements. In the future, therefore, these techniques will usher in a new era in which researchers can create novel species with designer chromosomes.
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Kazama Y., Ishii K., Hirano T., Wakana T., Yamada M., Ohbu S., Abe T.
Plant Journal 92 ( 6 ) 1020 - 1030 2017年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plant Journal
© 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd Heavy-ion irradiation is a powerful mutagen that possesses high linear energy transfer (LET). Several studies have indicated that the value of LET affects DNA lesion formation in several ways, including the efficiency and the density of double-stranded break induction along the particle path. We assumed that the mutation type can be altered by selecting an appropriate LET value. Here, we quantitatively demonstrate differences in the mutation type induced by irradiation with two representative ions, Ar ions (LET: 290 keV μm −1 ) and C ions (LET: 30.0 keV μm −1 ), by whole-genome resequencing of the Arabidopsis mutants produced by these irradiations. Ar ions caused chromosomal rearrangements or large deletions (≥100 bp) more frequently than C ions, with 10.2 and 2.3 per mutant genome under Ar- and C-ion irradiation, respectively. Conversely, C ions induced more single-base substitutions and small indels ( < 100 bp) than Ar ions, with 28.1 and 56.9 per mutant genome under Ar- and C-ion irradiation, respectively. Moreover, the rearrangements induced by Ar-ion irradiation were more complex than those induced by C-ion irradiation, and tended to accompany single base substitutions or small indels located close by. In conjunction with the detection of causative genes through high-throughput sequencing, selective irradiation by beams with different effects will be a powerful tool for forward genetics as well as studies on chromosomal rearrangements.
DOI： 10.1111/tpj.13738
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Sato Y., Hirano T., Ichida H., Murakami M., Fukunishi N., Abe T., Kawano S.
Journal of Applied Phycology 29 ( 5 ) 2287 - 2295 2017年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Applied Phycology
© 2017, Springer Science+Business Media Dordrecht. Undaria pinnatifida is grown for food and industrial materials worldwide; therefore, advanced breeding is needed to meet quality and productivity requirements. In this study, we examined regional lines of U. pinnatifida from five cultivation sites in Japan with different environmental conditions: Oga (OGA, the northern Sea of Japan coast), Hirota Bay (HRT, the northeastern Pacific coast), Matsushima Bay (MAT, the northeastern Pacific coast), Naruto (the Seto Inland Sea coast) and Shimonoseki (SIM, the southern Sea of Japan coast). The sporophytes of these lines were cultured in a tank culture system under controlled environmental conditions, and their morphological characteristics, nutrient uptake kinetics (V max , K s and V max /K s ), and carbon, nitrogen and phosphorus contents were determined. Sporophytes from MAT grew faster, whereas those from SIM were smaller than those from the other sites. Although the blade thickness of sporophytes cultivated in the sea significantly differs among cultivation sites in the previous study, there was no significant difference in blade thickness among the regional lines cultivated in the tank. Sporophytes from OGA had the greatest V max /K s values and significantly greater nitrogen contents than the other lines. Therefore, the morphological characteristics of MAT and SIM sporophytes, and the nutrient uptake kinetics of OGA sporophytes may have a genetic origin. This indicates that these lines may represent useful resources for selective breeding, with MAT sporophytes providing faster growth and OGA sporophytes being well-adapted to low-nutrient conditions.
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New cherry blossom cultivars induced by C-ion beam irradiation 査読あり
S. Ishii, Y. Hayashi, T. Hirano, Y. Kazama, T. Abe
RIKEN Accelerator Progress Report 50 275 2017年10月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
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Production of flower color mutants of spray-mum 'Southern Chelsea' by Ar-ion beam irradiation 査読あり
M. Tamari, Y. Tanokashira, Y. Hayashi, T. Hirano, T. Abe
RIKEN Accelerator Progress Report 50 274 2017年10月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
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Sato, Y., Yamaguchi, M., Hirano, T., Fukunishi, N., Abe, T., Kawano, S.
Journal of Applied Phycology 29 ( 3 ) 1429 - 1436 2017年6月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Applied Phycology
© 2016, Springer Science+Business Media Dordrecht. Kelps are economically valuable primary producers; therefore, many studies on breeding have attempted to increase kelp productivity and quality. However, most cultivation tests have been performed in the ocean, thereby limiting the development of new cultivars. To reduce the breeding duration period and confirm cultivar phenotypes, we developed a novel tank culture system, referred to as a circulation and floating culture system (CFCS), for cultivating macroalgae. In the CFCS, kelp can be cultivated under controlled environmental conditions. Water velocity in the CFCS can be regulated by changing the angle of a seawater inlet spout without changing the volume of seawater in the tank. Undaria pinnatifida and Saccharina japonica cultivated in the CFCS exhibited morphological features very similar to those of plants grown naturally in the ocean. The result suggests that the facility is useful for identifying water motion conditions suitable for increasing the production of any macroalgae species. Using this facility, both species were grown from juvenile sporophytes (20 mm) to maturity; for U. pinnatifida, the subsequent g eneration was successfully cultivated. Improved growth of U. pinnatifida was achieved in fast flows compared with slow flows, whereas S. japonica developed a wider shape and heavier biomass in slow flows compared with fast flows. We discuss the application and implication of the CFCS for breeding research and the physiological ecology of macroalgae.
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AMAP: A pipeline for whole-genome mutation detection in Arabidopsis thaliana.
Ishii K, Kazama Y, Hirano T, Hamada M, Ono Y, Yamada M, Abe T
Genes & genetic systems 91 ( 4 ) 229 - 233 2017年3月
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Induction of flower color mutants by heavy-ion irradiation to leaf blades of spray-mum 'Southern Chelsea' 査読あり
Y. Tanokashira, M. Tamari, S. Nagayoshi, Y. Hayashi, T. Hirano, T. Abe
RIKEN Accelerator Progress Report 49 261 2017年3月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
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Breeding of Summer-Autumn Flowering Chrysanthemum cv. Hakuryo with a little generation of malformed flower 査読あり
A. Hisamura, D. Mine, T. Takeda, T. Abe, Y. Hayashi, T. Hirano,
RIKEN Accelerator Progress Report 49 24 2017年3月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）