論文 - 平野 智也
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Early-flowering mutants developed by heavy-ion-beam irradiation in soybean 査読あり
H. Kuribara, Y. Sadamura, H. Watanuki, T. Hirano, Y. Hayashi, T. Abe
RIKEN Accelerator Progress Report 43 286 2010年9月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
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Sperm dimorphism in terms of nuclear shape and microtubule accumulation in Cyrtanthus mackenii 査読あり
Tomonari Hirano, Yoichiro Hoshino
Sexual Plant Reproduction 23 ( 2 ) 153 - 162 2010年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Dynamic morphologies of pollen plastids visualized by vegetative-specific FtsZ1–GFP in Arabidopsis thaliana 査読あり
Makoto T Fujiwara, Haruki Hashimoto, Yusuke Kazama, Tomonari Hirano, Yasushi Yoshioka, Seishiro Aoki, Naoki Sato, Ryuuichi D Itoh, Tomoko Abe
Protoplasma 242 ( 1-4 ) 19 - 33 2010年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sperm dimorphism in terms of nuclear shape and microtubule accumulation in Cyrtanthus mackenii 査読あり
Hirano T., Hoshino Y.
Sexual Plant Reproduction 23 ( 2 ) 153 - 162 2010年6月
掲載種別：研究論文（学術雑誌） 出版者・発行元：Sexual Plant Reproduction
Pollen tubes of Cyrtanthus mackenii, a species with bicellular pollen, were cultured in vitro to investigate nuclear phase changes during generative cell division and male germ unit (MGU) formation, using flow cytometric analysis. Results revealed that sperm cells were formed after 12 h of culture. During sperm maturation, the nuclei of sperm cells were not associated with the vegetative nucleus (unassociated sperm cells; Sua) and became longer than those of sperm cells associated with the vegetative nucleus (Svn). These findings indicate that the pair of sperm cells in the C. mackenii MGU is dimorphic in terms of nuclear shape. Dimorphism coincides with anti-α-tubulin antibody immunofluorescence, which was higher in the Sua than in Svn. Following treatment with oryzalin, triggering microtubule depolymerization, differences between nuclear shapes in the two sperm nuclei disappeared, suggesting that microtubule accumulation between sperm cells in the MGU correlates with differences in the nuclear shape. © Springer-Verlag 2009.
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Evaluation of Pre-Fertilization Barriers by Observation of Pollen Tube Growth and Attempts for Overcoming Post-Fertilization Barriers in Intergeneric Hybridization between Alstroemeria and Bomarea by Ovule Culture 査読あり
Y. Kashihara, T. Hirano, N. Murata, K. Shinoda, H. Araki, Y. Hoshino
Acta Horticulturae 855 159 - 164 2010年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Detection of changes in the nuclear phase and evaluation of male germ units by flow cytometry during in vitro pollen tube growth in Alstroemeria aurea 査読あり
Tomonari Hirano, Yoichiro Hoshino
Journal of Plant Research 122 ( 2 ) 225 - 234 2009年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Hirano T., Hoshino Y.
Journal of Plant Research 122 ( 2 ) 225 - 234 2009年3月
掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Plant Research
This study aimed to analyze male gamete behavior from mature pollen to pollen tube growth in the bicellular pollen species Alstroemeria aurea. For mature pollen, pollen protoplasts were examined using flow cytometry. The protoplasts showed two peaks of DNA content at 1C and 1.90C. Flow cytometry at different developmental stages of pollen tubes cultured in vitro revealed changes in the nuclear phase at 9 and 18 h after culture. Sperm cell formation occurred at 6-9 h after culture, indicating that the first change was due to the division of the generative cells into sperm cells. After sperm cell formation, the number of vegetative nucleus associations with sperm cells showed a tendency to increase. This association was suggested as the male germ unit (MGU). When sperm cells, vegetative nuclei, and partial MGUs were collected separately from pollen tubes cultured for 18 h and analyzed using a flow cytometer, the sperm cells and vegetative nuclei contained 1C DNA, while the DNA content of partial MGUs was counted as 2C. Therefore, the second change in the nuclear phase, which results in an increase in 2C nuclei, is possibly related to the formation of MGUs. © 2009 The Botanical Society of Japan and Springer.
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Cryopreservation and low temperature storage of seeds of Phaius tankervilleae 査読あり
Tomonari Hirano, Toshinari Godo, Kazumitsu Miyoshi, Keiko Ishikawa, Masaya Ishikawa, Masahiro Mii
Plant Biotechnology Reports 3 ( 1 ) 103 - 109 2009年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Cryopreservation of recalcitrant orchid seeds for long-term germplasm storage
Tomonari Hirano, Masahiro Mii
Proceedings 9th Asia Pacific Orchid Conference 404 - 408 2009年1月
記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス）
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Plant regeneration from suspension cells induced from hypocotyls derived from interspecific cross Alstroemeria pelegrina × A. magenta and transformation with Agrobacterium tumefaciens 査読あり
Yoichiro Hoshino, Yukiko Kashihara, Tomonari Hirano, Naho Murata, Koichi Shinoda
Plant Cell, Tissue and Organ Culture 94 ( 1 ) 45 - 54 2008年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Cryopreservation of immature seeds of Ponerorchis graminiforia var. suzukiana by vitrification 査読あり
Tomonari Hirano, Keiko Ishikawa, Masahiro Mii
Cyroletters 26 ( 3 ) 139 - 146 2005年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Cryopreservation of immature seeds of Bletilla striata by vitrification 査読あり
Tomonari Hirano, Toshinari Godo, Masahiro Mii, Keiko Ishikawa.
Plant Cell Reports 23 ( 8 ) 534 - 539 2005年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）