論文 - 平野 智也
-
Isolation of dwarf mutants induced with C-ion beam irradiation in pea cultivar "Kishu-usui" 査読あり
Y. Kotani, S. Ueyama, T. Hirano, T. Abe
RIKEN Accelerator Progress Report 49 264 2017年3月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
-
Sato Y., Hirano T., Niwa K., Suzuki T., Fukunishi N., Abe T., Kawano S.
Journal of Applied Phycology 28 ( 6 ) 3447 - 3458 2016年12月
掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Applied Phycology
Understanding the nutrient uptake kinetics of kelp populations will contribute to an improved understanding of environmental adaptation and the breeding of new cultivars. In this study, we examined the morphological characteristics, carbon (C) and nitrogen (N) contents, and NO<inf>3</inf> <sup>−</sup>–N and NH<inf>4</inf> <sup>+</sup>–N uptake kinetics of Undaria pinnatifida sporophytes cultivated at six industrial farms throughout Japan. We detected significant differences in morphology among sites. At Matsushima Bay (northern Pacific coast of Japan), where autumnal seawater temperatures fall in concert with increasing nutrient concentrations, sporophytes were significantly larger than at other sites from December to February. The C content of the sporophytes was seasonally stable at all of the locations, but the N content of sporophytes declined after February due to a decrease in seawater nutrients. We compared the uptake kinetics of NO<inf>3</inf> <sup>−</sup>–N and NH<inf>4</inf> <sup>+</sup>–N among cultivation sites. V<inf>max</inf> and K<inf>s</inf>, which are Michaelis–Menten parameters that measure adaptation to nutrient concentrations, were highest in the Seto Inland Sea and lowest in the northern sector of the Sea of Japan. The V<inf>max</inf>/K<inf>s</inf> ratio is a measure of adaptation to low nutrient concentrations; the highest values were measured in the northern sector of the Sea of Japan. The parameter ranges were broader than those previously reported for invasive populations of U. pinnatifida in other parts of the world. Thus, we detected population-level adaptations to the various nutrient conditions in Japanese waters, and these results suggest the existence of ecotypes according to nutrient uptake kinetics. The different populations may be used to provide sources of genetic material that could be of value in breeding programmes by improving productivity and quality.
-
レタス（Luctuca sativa L.）プロトプラストへのイオンビーム照射によって出現した低ポリフェノールオキシダーゼ活性を示す突然変異体 査読あり
澤田 倫平, 平野 智也, 飯牟禮 和彦, 阿部 知子, 尾崎 行生
園芸学研究 15 ( 4 ) 347 - 353 2016年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
-
Kotaro Ishii, Yusuke Kazama, Ryouhei Morita, Tomonari Hirano, Tokihiro Ikeda, Sachiko Usuda, Yoriko Hayashi, Sumie Ohbu, Ritsuko Motoyama, Yoshiaki Nagamura, Tomoko Abe
PLoS ONE 11 ( 7 ) e0160061 2016年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
-
New High-Energy Beam Transport Line Dedicated to Biological Applications in RIKEN RI Beam Factory 査読あり
Nobuhisa Fukunishi, Masaki Fujimaki, Misaki Komiyama, Keiko Kumagai, Takeshi Maie, Yutaka Watanabe, Tomonari Hirano, Tomoko Abe
Proceedings of the 13th International Conference on Heavy Ion Accelerator Technology 42 - 44 2016年3月
記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス）
-
Mana Katano, Kazuki Takahashi, Tomonari Hirano, Yusuke Kazama, Tomoko Abe, Hirokazu Tsukaya, Ali Ferjani
Frontiers in Plant Science 7 132 2016年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
-
Ion Beam Breeding and Gene Discovery for Function Analyses Using Mutants 査読あり
Abe T., Kazama Y., Hirano T.
Nuclear Physics News 25 ( 4 ) 30 - 34 2015年10月
掲載種別：研究論文（学術雑誌） 出版者・発行元：Nuclear Physics News
The RIKEN RI Beam Factory (RIBF) is the one of the biggest facilities to accelerate heavy ions in the world. Nuclear physics is the primary subject of the RIBF. You may know RIKEN as nuclear physics laboratories. Its activities cover every scientific and technological fields, even in agriculture. We have developed a unique technology for mutation induction by using heavy-ion beams from particle accelerators at the RIBE, through an efficient synergistic link between agricultural science and accelerator physics. At relatively low doses, ion beams induce mutations at a high rate without severely inhibiting growth. The irradiation treatment given to the various plant materials is quick, lasting between a few seconds and a few minutes, but is sufficient to induce mutation. By using this method, we already put 26 new cultivars on the market in Japan, the United States, Canada, and the European Union since 2001. For example, rose induced mutations are utilized in vegetative propagated crops to shorten the time necessary to breed for desirable characters (Figure 1). In the present article, we introduce the development and practical applications of ion-beam breeding, effects of Linear Energy Transfer (LET) on lethality and mutagenesis, and future perspectives on biological applications of heavy-ion beams.
-
Estimation method of microbeam divergence from glass capillaries for biological use 査読あり
R. J. Bereczky, T. Ikeda, T. Kobayashi, M. Utsugi, T. Hirano, Y. Sakai, T. Abe
RIKEN Accelerator Progress Report 48 316 2015年10月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
-
Ion track observation in cell nucleus irradiated by 3 MeV He ion microbeams produced with glass capillaries 査読あり
T. Ikeda, M. Izumi, V. Mäckel, T. Kobayashi, R. J. Bereczky, T. Hirano, Y. Yamazaki, T. Abe
RIKEN Accelerator Progress Report 48 315 2015年10月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
-
Rapid screening of heavy-ion-induced large deletion mutants by using quantitative real-time PCR in Arabidopsis thaliana 査読あり
Y. Kazama, T. Hirano, K. Ishii, M. Yamada, Y. Shirakawa, S. Ohbu, T. Abe
RIKEN Accelerator Progress Report 48 307 2015年10月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
-
Li Y., Abe A., Fuse T., Hirano T., Hoshino Y., Kunitake H.
Journal of the American Society for Horticultural Science 140 ( 4 ) 339 - 345 2015年7月
掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of the American Society for Horticultural Science
Self-incompatibility (SI) is an important mechanism in higher plants that promotes outcrossing and prevents self-fertilization. ‘Banpeiyu’ (Citrus maxima) and ‘Hyuganatsu’ (Citrus tamurana), two of the Citrus cultivars distributed in Kyusyu, Japan, show gametophytic SI. In this study, we used the Citrus mature pollen culture system and stylar crude protein extracts to simulate compatible (C) and SI responses in ‘Banpeiyu’ pollen tubes. We analyzed the protein changes in pollen tubes with the C- and SI-like treatments by nano-liquid chromatography-mass spectrometry (nano-LC-MS); 14 and 27 proteins were identified in C- and SI-like treatments, respectively. We picked up some candidate genes that were particularly prevalent in SI-like treatment and analyzed their expression level changes during C- and SI-like treatments in ‘Banpeiyu’ and ‘Hyuganatsu’ pollen tubes. The expression levels of copper/zinc superoxide dismutase (Cu/Zn SOD), manganese SOD (Mn SOD), catalase (CAT), and cysteine protease (CYP) increased after SI-like treatment. We used a fluorescent probe to visualize reactive oxygen species (ROS) level changes in ‘Banpeiyu’ and ‘Hyuganatsu’ pollen tubes after C- and SI-like treatments and found that 2-hour SI-like treatment induced ROS levels to increase in the pollen tubes of both cultivars. These results suggest that an ROS increase could be one of the key phenomena in the SI response of Citrus and that gene expression changes were responses to ROS generation.
-
Hirano T., Kazama Y., Ishii K., Ohbu S., Shirakawa Y., Abe T.
Plant Journal 82 ( 1 ) 93 - 104 2015年4月
掲載種別：研究論文（学術雑誌） 出版者・発行元：Plant Journal
Heavy-ion beams are widely used for mutation breeding and molecular biology. Although the mutagenic effects of heavy-ion beam irradiation have been characterized by sequence analysis of some restricted chromosomal regions or loci, there have been no evaluations at the whole-genome level or of the detailed genomic rearrangements in the mutant genomes. In this study, using array comparative genomic hybridization (array-CGH) and resequencing, we comprehensively characterized the mutations in Arabidopsis thaliana genomes irradiated with Ar or Fe ions. We subsequently used this information to investigate the mutagenic effects of the heavy-ion beams. Array-CGH demonstrated that the average number of deleted areas per genome were 1.9 and 3.7 following Ar-ion and Fe-ion irradiation, respectively, with deletion sizes ranging from 149 to 602 180 bp; 81% of the deletions were accompanied by genomic rearrangements. To provide a further detailed analysis, the genomes of the mutants induced by Ar-ion beam irradiation were resequenced, and total mutations, including base substitutions, duplications, in/dels, inversions, and translocations, were detected using three algorithms. All three resequenced mutants had genomic rearrangements. Of the 22 DNA fragments that contributed to the rearrangements, 19 fragments were responsible for the intrachromosomal rearrangements, and multiple rearrangements were formed in the localized regions of the chromosomes. The interchromosomal rearrangements were detected in the multiply rearranged regions. These results indicate that the heavy-ion beams led to clustered DNA damage in the chromosome, and that they have great potential to induce complicated intrachromosomal rearrangements. Heavy-ion beams will prove useful as unique mutagens for plant breeding and the establishment of mutant lines. Significance Statement Heavy-ion beams, a form of ionizing radiation, efficiently induced both large deletion mutations and genomic rearrangements. The heavy-ion beams led to clustered DNA damage in the chromosome, and that they have a great potential to induce complicated intrachromosomal rearrangements.
DOI： 10.1111/tpj.12793
-
Effects of Polyamines on Self-incompatibility-like Responses in Pollen Tubes of Citrus Cultivars, Banpeiyu and Hyuganatsu. 査読あり
Yiran Li, Asuka Uchida, Akiha Abe, Akihiro Yamamoto, Tomonari Hirano, Hisato Kunitake
Journal of the American Society for Horticultural Science 140 ( 2 ) 183 - 190 2015年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
-
Observation of unrepairable lesions at DNA by using 3-MeV proton microbeams produced by glass capillaries 査読あり
T. Ikeda, M. Izami, V. Mäckel, T. Kobayashi, K. Ogawa, T. Hirano, Y. Yamazaki, T. Abe
RIKEN Accelerator Progress Report 47 282 2015年2月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
-
Effects of heavy-ion beam irradiation on sporophyte survival and growth in Undaria pinnatifida 査読あり
Y. Sato, T. Hirano, Y. Hayashi, M. Kasahara, N. Fukunishi, T. Abe, S. Kawano
RIKEN Accelerator Progress Report 47 300 2015年2月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
-
Rapid evaluation of mutational effects of heavy-ion irradiation to Undaria pinnatifida 査読あり
T. Hirano, Y. Sato, K. Ichinose, Y. Hayashi, N. Fukunishi, T. Abe
RIKEN Accelerator Progress Report 47 299 2015年2月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
-
Effects of heavy-ion-beam irradiation on flower-color mutation in chrysanthemum 査読あり
Y. Tanokashira, S. Nagayoshi, T. Hirano, T. Abe
RIKEN Accelerator Progress Report 47 297 2015年2月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
-
Flower color mutants of chrysanthemum obtaioned using C-ion beam irradiation 査読あり
K. Tamaki, M. Yamanaka, Y. Mizutani, Y. Hayashi, T. Hirano, T. Abe
RIKEN Accelerator Progress Report 47 296 2015年2月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
-
Effect of C-ion beam irradiation on survival rates and flower color mutations in statice (Limonium sinuatum Mill.) 査読あり
D. Ogawa, T. Fujioka, T. Hirano, T. Abe
RIKEN Accelerator Progress Report 47 295 2015年2月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）
-
Relationship between gene expression level and LET immediately after heavy-ion beam irradiation in rice 査読あり
K. Ishii, R. Moria, T. Hirano, Y. Kazama, T. Ikeda, Y. Nagamura, T. Abe
RIKEN Accelerator Progress Report 47 290 2015年2月
記述言語：英語 掲載種別：研究論文（その他学術会議資料等）