Papers - HAYASHI Masahiro
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Regulation of TG accumulation and lipid droplet morphology by the novel TLDP1 in Aurantiochytrium limacinum F26-b Reviewed
Takashi Watanabe, Ryo Sakiyama, Yuya Iimi, Satomi Sekine, Eriko Abe, Kazuko H. Nomura, Kazuya Nomura, Yohei Ishibashi, Nozomu Okino, Masahiro Hayashi, Makoto Ito
Journal of Lipid Research 58 2334 - 2347 2017.10
Language：English Publishing type：Research paper (scientific journal)
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Degradation of distillery lees (Shochu kasu) by cellulase-producing thraustochytrids Reviewed
Taoka Y., Nagano N., Kai H., Hayashi M.
Journal of Oleo Science 66 ( 1 ) 31 - 40 2017.5
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Oleo Science
© 2017 by Japan Oil Chemists’ Society. Single cell oils produced by oleaginous microorganisms have attracted increasing interests as a petroleum alternative energy. Marine eukaryotes, thraustochytrids were heterotrophic, and can grow rapidly and accumulate large amount of lipids containing functional fatty acids, such as docosahexaenoic acid (DHA) in their cells body. In this investigation, thraustochytrids isolated from marine environment were cultured in the medium containing an industrial waste and an unused resource, distillery lees (Shochu kasu) to produce biofuel or functional fatty acids by microorganisms. Sixty-nine thraustochytrids and Schizochytrium aggregatum ATCC 28209 were screened for cellulase production, and the activities were detected using sodium carboxymethyl cellulose (CMC) as a substrate. Based on the screening test, strain TM02Bc identified to Schizochytrium sp. was selected for the Shochu kasu degradation test and compared with S. aggregatum ATCC 28209 previously known as a cellulase-producing thraustochytrid. Strains TM02Bc and ATCC 28209 were cultured in artificial seawater containing Shochu kasu for 15 days. The two strains could degrade Schochu kasu, especially that from sweet potato Shochu (Imo Shochu). Cellulase (CMCase) and protease activities were detected in culture supernatant of both strains, and the ratio of polyunsaturated fatty acids (PUFAs) significantly increased as a result of incubation of Shochu kasu with two strains. This preliminary study indicated that strain TM02Bc was a potent candidate for Shochu kasu treatment and fatty acid production.
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Lipid Droplet-Related Protein 1 (LDRP1) Regulates the Triacylglycerol Accumulation and Lipid Droplet Morphology in a Thraustochytrid, Aurantiochytrium limacinum F26-b Reviewed
Takashi Watanabe1, Ryo Sakiyama1, Yuya Iimi, Satomi Sekine1, Eriko Abe, Kazuko H. Nomura, Kazuya Nomura, Yohei Ishibashi, Nozomu Okino, Masahiro Hayashi, Makoto Ito
Journal of Biological Chemistry 2017.1
Language：English Publishing type：Research paper (scientific journal)
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微細藻類プラスチックの開発 Invited Reviewed
林 雅弘、芝上 基成
化学工学 80 ( 5 ) 2 - 4 2016.12
Authorship：Lead author Language：Japanese Publishing type：Research paper (scientific journal)
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Synthesis of nanofiber-formable carboxymethylated Euglena-derived beta-1,3-glucan Reviewed
Motonari Shibakami, Gen Tsubouchi, Mitsugu Sohma, Masahiro Hayashi
Carbohydrate Polymers 152 468 - 478 2016.6
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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微細藻類とホワイトバイオテクノロジー Reviewed
末永智幸、林 雅弘
応用細胞生物学研究 28 ( 1 ) 10 - 14 2015.12
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)
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Water-soluble jack-knife prawn extract inhibits 5-hydroxytryptamine-induced vasoconstriction and platelet aggregation in humans Reviewed
Shuji Gamoh, Tasuku Kanai, Naoko Tanaka-Totoribe, Masamichi Ohkura, Masachika Kuwabara, Eisaku Nakamura, Atsuko Yokota, Tetsuo Yamasaki, Akiko Watanabe, Masahiro Hayashi, Shouichi Fujimoto and Ryuichi Yamamoto
Food & Function 6 444 - 449 2015.12
Language：English Publishing type：Research paper (scientific journal)
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油糧海洋微生物ラビリンチュラの産業利用 Invited Reviewed
林 雅弘、松田綾子、松田高宜、泉 可也
酵素工学ニュース ( 74 ) 6 - 9 2015.10
Authorship：Lead author Language：Japanese Publishing type：Research paper (scientific journal)
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Preparation of transparent self-standing thin films made from acetylated euglenoid β-1,3-glucans Reviewed
Shibakami M., Tsubouchi G., Sohma M., Hayashi M.
Carbohydrate Polymers 133 421 - 428 2015.8
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Carbohydrate Polymers
© 2015 Elsevier Ltd. All rights reserved. The feasibility of using acetylparamylon to prepare self-standing optical films was investigated. Its thermal stability, represented by the glass transition temperature, and 5% weight loss temperature are equivalent to those of acetylcellulose. The mechanical properties (maximum stress, elongation at break, and elastic modulus) of acetylparamylon films prepared by solution casting were comparable to those of acetylcellulose films. The degrees of their transparency and birefringence were the same as those of the acetylcellulose film. These results, together with the finding that acetylparamylon with a higher degree of acetylation formed a thin film with fewer micrometer-sized defects and that acetylparamylon with a lower degree of acetylation formed a thin film with a nanometer-level flat surface, suggest that acetylparamylon is a feasible material for self-standing optical film.
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油糧微生物ラビリンチュラ Invited Reviewed
林 雅弘
生物工学 93 ( 7 ) 426 - 429 2015.7
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)
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One-pot synthesis of thermoplastic mixed paramylon esters using trifluoroacetic anhydride Reviewed
Motonari Shibakami, Gen Tsubouchi, Masahiro Hayashi
Carbohydrate Polymers 119 1 - 7 2015.4
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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林 雅弘
生物工学会誌 : seibutsu-kogaku kaishi 93 ( 7 ) 426 - 429 2015
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：日本生物工学会
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Abe E., Ikeda K., Nutahara E., Hayashi M., Yamashita A., Taguchi R., Doi K., Honda D., Okino N., Ito M.
PLoS ONE 9 ( 8 ) 2014.8
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：PLoS ONE
N-3 polyunsaturated fatty acids (PUFA), such as docosahexaenoic acid (DHA, 22:6n-3), have been reported to play roles in preventing cardiovascular diseases. The major source of DHA is fish oils but a recent increase in the global demand of DHA and decrease in fish stocks require a substitute. Thraustochytrids, unicellular marine protists belonging to the Chromista kingdom, can synthesize large amounts of DHA, and, thus, are expected to be an alternative to fish oils. DHA is found in the acyl chain(s) of phospholipids as well as triacylglycerols in thraustochytrids; however, how thraustochytrids incorporate DHA into phospholipids remains unknown. We report here a novel lysophospholipid acyltransferase (PLAT1), which is responsible for the generation of DHA-containing phosphatidylcholine and phosphatidylethanolamine in thraustochytrids. The PLAT1 gene, which was isolated from the genomic DNA of Aurantiochytrium limacinum F26-b, was expressed in Saccharomyces cerevisiae, and the FLAG-tagged recombinant enzyme was characterized after purification with anti-FLAG affinity gel. PLAT1 shows wide specificity for donor substrates as well as acceptor substrates in vitro, i.e, the enzyme can adopt lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylserine and lysophosphatidylinositol as acceptor substrates, and 15:0/16:0-CoA and DHA-CoA as donor substrates. In contrast to the in vitro experiment, only lysophosphatidylcholine acyltransferase and lysophosphatidylethanolamine acyltransferase activities were decreased in plat1-knockout mutants, resulting in a decrease of 16:0-DHA-phosphatidylcholine (PC) [PC(38:6)] and 16:0-DHA-phosphatidylethanolamine (PE) [PE(38:6)] , which are two major DHA-containing phospholipids in A. limacinum F26-b. However, the amounts of other phospholipid species including DHA-DHA-PC [PC(44:12)] and DHA-DHA-PE [PE(44:12)] were almost the same in plat-knockout mutants and the wild-type. These results indicate that PLAT1 is the enzyme responsible for the generation of 16:0-DHA-PC and 16:0-DHA-PE in the thraustochytrid. © 2014 Abe et al.
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Thermoplasticization of euglenoid beta-1,3-glucans by mixed esterification Reviewed
Motonari Shibakami, Gen Tsubouchi, Masahiro Hayashi
Carbohydrate Polymers 105 90 - 96 2014.5
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
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ミドリムシを主原料としたバイオプラスチックの開発 Invited Reviewed
芝上基成、林 雅弘
プラスチックエージ ( 12 ) 92 - 96 2013.12
Authorship：Lead author Language：Japanese Publishing type：Research paper (other academic)
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Effect of graded levels of dietary DHA included in rotifers Brachionus plicatilis on laviculture performance of red sea bream Pagrus major Reviewed
Kotani, T., H. Fushimi, Y. Ohta, A. Miyashita, K. Sudoh, M. Hayashi, N. Satoh, S. Satoh
Aquaculture Science 61 ( 4 ) 321 - 330 2013.12
Language：English Publishing type：Research paper (scientific journal)
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藻類を活用する食品素材開発 Invited Reviewed
林 雅弘
生物工学 91 ( 11 ) 621 - 624 2013.11
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)
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Food material development to take advantage of algae Invited Reviewed
Hayashi M.
Seibutsu-kogaku Kaishi 91 ( 11 ) 621 - 624 2013.11
Authorship：Lead author, Corresponding author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Seibutsu-kogaku Kaishi
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ミドリムシプラスチックとミドリムシナノファイバーの開発 Invited Reviewed
芝上基成、林 雅弘
自動車技術 67 ( 9 ) 88 - 89 2013.9
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (other academic)
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Effect of trace elements on growth of marine eukaryotes, tharaustochytrids Reviewed
Nagano Naoki, Taoka Yousuke, Honda Daiske, Hayashi Masahiro
Journal of bioscience and bioengineering 116 ( 3 ) 337 - 339 2013.9
Authorship：Corresponding author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：公益社団法人日本生物工学会
We determined the effect of trace elements on the growth of thraustochytrids. The growth of the strains cultured with the trace elements was much higher than that of the strains cultured without any trace element. Iron and zinc were particularly important to obtaining the optimum growth of thraustochytrids.