Papers - HAYASHI Masahiro
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伏見 浩, 小谷 知也, 鈴木 久英, Hayashi M.
福山大学内海生物資源研究所報告 16 18 - 20 2005.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：福山大学
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Adachi S., Miyamoto E., Watanabe F., Enomoto T., Kuda T., Hayashi M., Nakano Y.
Journal of Liquid Chromatography and Related Technologies 28 ( 16 ) 2561 - 2569 2005.10
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of Liquid Chromatography and Related Technologies
Significant amounts of vitamin B 12 (about 116.3 ∼ 556.3 μg/100 g) were determined in a Japanese salted and fermented salmon kidney "Mefun." A corrinoid compound was purified to homogeneity from Mefun and partially characterized. TLC and HPLC patterns of the purified corrinoid compound were identical to those of authentic vitamin B 12 , but not to inactive corrinoids. The vitamin B 12 found in Mefun was not derived from concomitant vitamin B 12 -synthesizing bacteria, but had accumulated in the salmon kidney. Gel filtration experiments demonstrated that most of the vitamin B 12 found in Mefun was recovered in the free vitamin B 12 fractions. These results indicate that Mefun would be an excellent vitamin B 12 (free form) source for elderly persons with food-bound vitamin B 12 malabsorption. Copyright © Taylor & Francis, Inc.
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ドコサヘキサエン酸富化スピルリナSpilurina platensis の調製と脂質特性
共著者：雪野繼代, 林 雅弘, 井上良計, 今村純子, 長野直樹, 村田 寿
日本水産学会誌71巻 1号74-79頁 2005.1
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Incorporation of exogenous docosahexaenoic acid into triacylglycerols and polar lipids of Chlorella vulgaris
共著者：T. Yoshimatsu, T. Higuchi, D. Zhang, Y. Hamasaki, K. Tanaka, T. Yukino, M. Hayashi
Journal of OleoscienceVol. 54, No. 115-19 2005.1
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YUKINO TUGIYO, HAYASHI MASAHIRO, INOUE YOSHIKAZU, IMAMURA JUNKO, NAGANO NAOKI, MURATA HISASHI, TSUGIYO YUKINO, MASAHIRO HAYASHI, YOSHIKAZU INOUE, JUNKO IMAMURA, NAOKI NAGANO, HISASHI MURATA, United Graduate School of Agriculture Kagoshima University:(Present address)University of Miyazaki, Department of Biological Production and Environmental Science Faculty of Agriculture University of Miyazaki, CycloChem Co. Ltd, Bizen Chemical Co. Ltd., Department of Biological Production and Environmental Science Faculty of Agriculture University of Miyazaki, Department of Biological Production and Environmental Science Faculty of Agriculture University of Miyazaki
NSUGAF 71 ( 1 ) 74 - 79 2005.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Fisheries Science
Nutritional fortification of <i>Spirulina</i> with docosahexaenoic acid (DHA) was attempted. Fish oil, DHA-ethyl and free DHA were supplemented into the culture of <i>Spirulina</i> as a source of DHA. Exogenous DHA in triglycerides and ethyl ester were not taken up into <i>Spirulina</i> cells. However, the cells took up the DHA in free form from the culture media. The cells without supplementation with DHA did not contain DHA in the cellular fatty acids. The DHA content of the cells was greatly increased by supplementation with DHA into the culture media. It reached to 70.2% of the total fatty acid in the cells.<br> DHA was mainly incorporated into the neutral lipids such as monoglycerides and diglycerides in the cells. Free fatty acids were slightly detected in the DHA-fortified cells. A small amount of DHA was also incorporated into glycolipid and phospholipid fractions. Sulfoquinovosyldiacylglycerol in the DHA-fortified <i>Spirulina</i> contained 9.1% of DHA in the total fatty acid. However, only 1-2% of DHA were incorporated into the other polar lipids.<br>
DOI： 10.2331/suisan.71.74
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Incorporation of exogenous docosahexaenoic acid into triacylglycerols and polar lipids of Chlorella vulgaris
共著者：T. Yoshimatsu, T. Higuchi, D. Zhang, Y. Hamasaki, K. Tanaka, T. Yukino, M. Hayashi
Journal of OleoscienceVol. 54, No. 115-19 2005.1
Language：Japanese Publishing type：Research paper (scientific journal)
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ドコサヘキサエン酸富化スピルリナSpilurina platensis の調製と脂質特性
共著者：雪野繼代, 林 雅弘, 井上良計, 今村純子, 長野直樹, 村田 寿
日本水産学会誌71巻 1号74-79頁 2005.1
Language：Japanese Publishing type：Research paper (scientific journal)
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Yukino T., Hayashi M., Maruyama I., Murata H.
Journal of Oleo Science 54 ( 1 ) 15 - 19 2005
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of Oleo Science
Docosahexaenoic acid (DHA)-enriched Chlorella vulgaris is commonly used as a feed in fisheries and as a dietary supplement. The time course DHA uptake into the Chlorella cells and incorporation of DHA into the triacylglycerols (TAG) and polar lipids (PL) were examined. After the addition of DHA into the Chlorella culture, DHA was rapidly taken up by the Chlorella cells and was mainly incorporated into TAG. However, some DHA was also incorporated into PL molecules. Although the percentage of DHA in the total fatty acids of total cellular lipids reached at 67%, the percentage in the total fatty acids of PL was 27.5%. As for the effects of pH, exogenous DHA was mainly incorporated into TAG molecules at pH 5-7. At pH 3-4 and 8, the DHA accumulated as free fatty acid in the cells. The exogenous DHA was rapidly incorporated into TAG molecules within 24 hours at pH 5-8. The findings should contribute profitably to production of better fishery feed and food supplement by using DHA-enriched Chlorella. © 2005, Japan Oil Chemists' Society. All rights reserved.
DOI： 10.5650/jos.54.15
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DHA強化クロレラ Chlorella vulgaris K-22 によるワムシ培養と栄養強化
雪野 繼代, 林 雅弘, 吉松 隆夫, 丸山 功, 村田 寿
水産増殖 52 ( 4 ) 381 - 386 2004.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：水産増殖談話会
ドコサヘキサエン酸（DHA）を強化したクロレラ Chlorella vulgaris K-22によるワムシ培養の効果を検討した。各種濃度でDHAを強化したクロレラ細胞には主として中性脂質にDHAが取り込まれており、総脂質中のDHA含有率は最高82.9％に達した。DHA強化クロレラを給餌したワムシ中には6時間で乾物中3.3％のDHAが蓄積し、短時間でワムシの栄養強化が可能であった。また、3日間によるワムシ培養では通常のクロレラ給餌より高い増殖が得られた。培養期間中、ワムシ中のDHA含有量は安定して高い値を示し、DHA強化クロレラ給餌によるワムシ培養で、栄養強化処理の必要ないレベルのDHAを含有するワムシを安定して得ることができた。
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Nutritional enrichment and cultivation of rotifers by feeding of docosahexaenoic acid enriched Chlorella vulgaris K-22
共著者：Yukino, T., Hayashi, M., Yoshimatsu, T., Maruyama, I., Murata, H.
SuisanzoushokuVol. 52, No. 4381-386 2004.8
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Yasmin Afsana, Takeuchi Toshio, Hayashi Masahiro, HIROTA TETSUYA, ISHIZUKA WAICHI, ISHIDA SHUZO
Fisheries science : FS 70 ( 3 ) 473 - 481 2004.6
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Fisheries Science
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A Technique to Enrich α-tocopherol in Clilorella and Marine Rotifers
NAGANO Naoki, HAYASHI Masahiro, YUKINO Tsugiyo, MURATA Hisashi
水産増殖 = The aquiculture 51 ( 4 ) 459 - 460 2003.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：日本水産増殖学会
α-Tocopherol (TOC) and various fatty acids were supplemented into the <I>Chlorella vulgaris</I> strain K-22 culture at 28°C for 24 h, and TOC content of the <I>Chlorella</I> cells was determined. The addition of TOC alone into the <I>Chlorella</I> culture medium did not give an increase of the cellular TOC content. However, the TOC contents in the <I>Chlorella</I> cells supplemented with TOC and various fatty acids were much higher than those supplemented with TOC alone. Moreover, the TOC content in the cells supplemented with a mixture of TOC and oleic acid was higher than those supplemented with a mixture of TOC and docosahexaenoic acid (DHA) . The TOC content of the rotifers fed on the TOC-enriched <I>Chlorella</I> was much higher than rotifers fed on unenriched <I>Chlorella</I>. On the other hand, rotifers could be easily enriched with TOC by feeding the TOC-enriched <I>Chlorella</I> cells.
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Ji Hong, Om Ahmad Daud, Yoshimatsu Takao, HAYASHI MASAHIRO, UMINO TETSUYA, NAKAGAWA HEISUKE, ASANO MASAYA, NAKAGAWA ATSUSHI
Fisheries science : FS 69 ( 5 ) 1001 - 1009 2003.10
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Fisheries Science
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NOGUCHI Satoko, AKIYAMA Jun, HADA Takahiro, INOUE Yoshikazu, ARAKI Shigeru, YUKINO Tsugiyo, HAYASHI Masahiro, TADA Mikiro, TAKAHATA Kyoya
Japanese journal of food chemistry 10 ( 2 ) 101 - 107 2003.8
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Food Chemistry
About 70% of total lipids in porphyra yezoensis, which is processed for use as edible sea alga, is composed with three types of glycolipids : MGDG (monogalactosyldiacylglycerol), DGDG (digalactosyldiacylglycerol), and SQDG (sulfoquinovosylgalactosyldiacylglycerol). In these glycolipids, SQDG is reported as an inhibitor of DNA synthesis. In this paper, with the aim of studying more about the physiological functions of SQDG extracted from sea alga (A-SQDG), we investigate its effects on the growth and differentiation of murine neuroblastoma cells (Neuro2a). These glycolipids (MGDG, DGDG, and SQDG) extracted from sea alga inhibited the cell growth of Neuro2a, but this inhibition was most potent in A-SQDG, which was extracted from alga. We therefore compared this inhibitory effect of A-SQDG with S-SQDG or C-SQDG extracted from spinach or chlorella, respectively. It was found that A-SQDG and S-SQDG had an equivalent inhibitory effect on cell growth, but C-SQDG had none at all. Furthermore, we investigated the effects of these three types of SQDG on neuritogenesis in Neuro2a cells. We found that A-SQDG revealed a potent effect on neuritogenesis than that by retinoic acid (RA) known as a potent reagent of neuritogenesis. It was also found that S-SQDG revealed the same degree of neuritogenesis as RA, but no neuritogenesis could be found in C-SQDG. The different effects of these three types of SQDG are thought to be originated in their molecular compositions of acyl group. The caspase-3 activity was known as a maker enzyme of apoptosis. As a result, A-SQDG, S-SQDG and C-SQDG almost inhibited etoposide-induced apoptosis. These SQDG were expected to be reagents to prevent neuronal cell death in Alzheimer's disease.
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Preparation and Utilization of the Chlorella Cells Enriched with Docosahexaenoic Acid
HAYASHI Masahiro
NIPPON SHOKUHIN KOGYO GAKKAISHI 50 ( 6 ) 289 - 292 2003.6
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Society for Food Science and Technology
DOI： 10.3136/nskkk.50.289
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ドコサヘキサエン酸富化Chlorella vulgaris CK22株のヒトにおける安全性および血清脂質濃度に対する影響の予備的検討
共著者田中邦明，小西史子，丸山 功，雪野繼代，木柎久雄，熊谷多妙子，羽田尚彦，林 雅弘
日本栄養・食糧学会誌，55巻，6号，323-330頁 2002.12
Language：Japanese Publishing type：Research paper (scientific journal)
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ドコサヘキサエン酸富化Chlorella vulgaris CK22株の脂質特性
共著者：雪野繼代，田中邦明，丸山 功，小西史子，熊谷多妙子，羽田尚彦，林 雅弘
日本栄養・食糧学会誌，55巻，6号，331-337頁 2002.12
Language：Japanese Publishing type：Research paper (scientific journal)
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Distribution of docosahexaenoic acid in DHA-enriched Euglena gracilis
共著者：Hayashi, M., Yukino, T., Park, B. S.
Fisheries Science, Vol. 68, Supplement 1,1002-1003 2002.11
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A preliminary experiment on the dietary value of vitamin E-fortified Chlorella for rotifer
共著者Yoshimatsu, T., Furusawa, T., Hayashi, M.：
Fisheries Science, Vol. 68, Supplemen1,1004-1005 2002.11
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Effect of Cobalt Compound on Population Growth of Rotifer
共著者：Yoshimatsu, T., Higuchi, T., Shirakawa, K., Hayashi, M., Tanaka, K., Yoshimura, K.
Proceeding of the 5th Korea-Japan (Japan-Korea) Biannual Joint Meeting, Kunsan National University, Kunsan, Koreap68 2002.10