Papers - HAYASHI Masahiro
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Yoshimatsu T., Furuichi M., Kitajima C., Hayashi M., Toda K.
Nippon Suisan Gakkaishi (Japanese Edition) 61 ( 6 ) 912 - 918 1995
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Nippon Suisan Gakkaishi (Japanese Edition)
A feeding experiment for redlip mullet larvae (4.58 ±0.25 mm TL) on the EFA requirement was conducted by using rotifers enriched with ethyl esters of four kinds of fatty acids: linoleic acid, LA; linolenic acid, LNA; eicosapentaenoic acid, EPA; docosahexaenoic acid, DHA. Larvae receiving rotifers enriched with LA and LNA showed typical symptoms of EFA deficiency syndromes, and obtained poor growth and high mortality. On the other hand, the larvae receiving rotifers enriched with EPA and/or DHA showed better growth, activity, and survival rates. These results suggested that the redlip mullet larvae required n-3HUFA like EPA and DHA as their essential fatty acids. Also, the supplemental effect of unicellular alga Nannochloropsis sp. to rearing water was investigated from the view point of EFA requirement. The n-3HUFA content in rotifer cultured with Nannochloropsis decreased linearly after they transferred into sea water. However, when 40–50 x 10 4 cells/m/ of Nannochloropsis was introduced to a rearing tank, the n-3HUFA content in rotifer remained without decreasing for 6 hours. The larvae reared in the tank with Nannochloropsis supplement showed better growth than those of non- or fewer-supplement groups. © 1995, The Japanese Society of Fisheries Science. All rights reserved.
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Effects of shifting pH in thestationary phase of growth on the hemical composition of Euglena racilis Reviewed
Hayashi, M., Toda, K., Ishiko, H., Komatsu, R., Kitaoka, S.
Biosci. Biotechnol. Biochem., Vol. 58, No. 11, 1964-1967 58 ( 11 ) 1964 - 1967 1994.11
Language：English Publishing type：Research paper (scientific journal)
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ユーグレナ Euglena gracilis による生物餌料の栄養強化とマダイ仔魚に対する餌料価値 Reviewed
林 雅弘，戸田享次，米司 隆，佐藤 修，北岡正三郎
日本水産学会誌 59 ( 6 ) 1051 - 1058 1993.6
Language：Japanese Publishing type：Research paper (scientific journal)
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エイコサペンタエン酸およびドコサヘキサエン酸強化 Euglena gracilisの調製
林 雅弘，戸田享次，三澤嘉久，北岡正三郎
水産増殖 41 ( 2 ) 169 - 176 1993.6
Language：Japanese Publishing type：Research paper (scientific journal)
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Enriching Euglena with unsaturated fatty acids Reviewed
Hayashi, M., Toda, K., Kitaoka, S.
Biosci. Biotechnol. Biochem., Vol. 57,No.2, 352-353 57 ( 2 ) 352 - 353 1993.2
Language：English Publishing type：Research paper (scientific journal)
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Dietary Value of Rotifers and Artemia Enriched with Euglena gracilis for Red Sea Bream
Hayashi M., Toda K., Yoneji T., Sato O., Kitaoka S.
NIPPON SUISAN GAKKAISHI 59 ( 6 ) 1051 - 1058 1993
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：NIPPON SUISAN GAKKAISHI
Since Euglena gracilis incorporate and accumulate n-3 highly unsaturated fatty acids (n-3 HUFA), the fatty acid composition of the cells can be controlled by supplementation of the fatty acids into growth media. Rotifers and Artemia were enriched with Euglena that accumulated docosahexaenoic acid (DHA). The DHA content of the rotifers fed on more than 100000 cells/m/ of Euglena increased in 12 hours. Also, the feeding density of more than 100000 cells/m/ gave an increase in the DHA content in Artemia. As for Artemia, however, DHA was degraded fast after 6 hours of enrichment. A feeding experiment was conducted to evaluate the dietary value of living feed enriched with Euglena. Rotifers and Artemia enriched with Euglena contained much more DHA than those enriched with Nannochloropsis or methyl esters of n-3 HUFA. The larval red sea bream fed on living feed containing more DHA showed higher activity in the activity test. Thus, the dietary value of rotifers and Artemia to red sea bream was effectively improved by enrichment with Euglena. Euglena is expected to be profitable feed for the DHA enrichment of rotifers and Artemia. © 1993, The Japanese Society of Fisheries Science. All rights reserved.
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微生物によるワックスエステルの生産
奥村昌和,井伊 茂,市川伸哉,林 雅弘
油化学, 36巻, 10号, 802-807頁 36 ( 10 ) 802 - 807 1987.10
Language：Japanese Publishing type：Research paper (scientific journal)