Papers - HAYASHI Masahiro
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Increase of EPA in Thraustochytrids through Expression of a Fatty Acid 5 Desaturase Gene driven by the Thraustochytrid Ubiquitin Promoter
Takumi Kobayashi1, Keishi Sakaguchi1, Takanori Matsuda1, Eriko Abe1, Yoichiro Hama2, Masahiro Hayashi3, Daisuke Honda4, Yuji Okita5, Shinichi Sugimoto5,Nozomu Okino1 and Makoto Ito1,6,
Journal of Biochemistry 2011.1
Language：Japanese Publishing type：Research paper (scientific journal)
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Use of an antifungal drug, amphotericin B for isolation of thraustochytrids
TAOKA Yousuke, NAGANO Naoki, OKITA Yuji, IZUMIDA Hitoshi, SUGIMOTO Shinichi, HAYASHI Masahiro
Journal of bioscience and bioengineering 110 ( 6 ) 720 - 723 2010.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Society for Biotechnology, Japan
The inhibitory effect of amphotericin B (AMPH) on the growth of fungi during the isolation of thraustochytrids was examined. The growth of fungi was significantly inhibited by addition of AMPH, and therefore colonies of thraustochytrids were not overlaid with fungal mycelia, which resulted in increased efficiency of thraustochytrids isolation.
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Effect of nutritional enrichment method on fatty acid contents of rotifer Brachionus plicatilis
Kotani T., Genka T., Tanabe M., Miyashima A., Fushimi H., Hayashi M.
Journal of the World Aquaculture Society 41 ( 6 ) 884 - 892 2010.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of the World Aquaculture Society
Nutritional enrichment for rotifer is necessary for the stable production in larviculture. Although manufacturers recommend feeding amount for enrichment diets, the effects of these recommended amounts on nutritional enrichment of rotifer are unknown. We assessed the effects of normal and overdose amount of enrichment diets on nutritional enrichment of rotifer using batch and continuous culture methods. Rotifer populations in two culture systems were enriched using four enrichment conditions, matched two diet levels, and two enrichment durations: 0.25 g/L for 8 h (normal enrichment) 0.25 g/L for 24 h (longer enrichment), 0.75 g/L for 8 h (overdose enrichment), and 0.75 g/L for 24 h (overdose + longer enrichment). When the batch-cultured rotifer populations were enriched, longer and overdose enrichments could not make the fatty acid contents differ from the normal one. Overdose enrichment was most effective of all enrichment conditions when continuous-cultured rotifer populations were used. In both culture methods, the docosahexaenoic acid (DHA) content under overdose + longer enrichment conditions was two to three times as high as normal enrichment conditions and it resulted in two- to threefold increase in DHA/eicosapentaenoic acid ratio by overdose + longer enrichment. Accordingly, it is possible to alter the fatty acid composition of rotifer by changing the enrichment method. © by the World Aquaculture Society 2010.
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物質生産ツールとしての微細藻 : 電子指向型バイオテクノロジー(e-バイオ)を起点とする藻類工学の夜明け(第62回大会シンポジウム報告)
石井 正治, 林 雅弘
生物工学会誌 : seibutsu-kogaku kaishi 88 ( 12 ) 2010
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：日本生物工学会
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Nagano N., Taoka Y., Honda D., Hayashi M.
Journal of Oleo Science 58 ( 12 ) 623 - 628 2009.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of Oleo Science
The effects of carbon sources, seawater concentration and seawater component in a culture medium were investigated to optimize culture conditions for growth by a marine thraustochytrid Aurantiochytrium limacinum strain mh0186. Strain mh0186 could utilize D-glucose, D-fructose and D- mannose as carbon sources. Seawater concentrations between 12.5 - 200% were required for good growth, and a single omission of magnesium sulfate from the seawater reduced the growth of the cells. Jar fermentor trials were carried out for the purpose of docosahexaenoic acid (DHA) production by strain mh0186. The total fatty acid content of the cell was 466.5 mg/g dry cells, and biomass and DHA yield were estimated as 23.1 g/L and 4.3 g/L, respectively, at 26 h. The daily production of DHA by the strain was 4.0 g/L/d, suggesting that the higher DHA production rate of our strain mh0186 should be appropriate for industrial production of DHA. © 2009 by Japan Oil Chemists' Society.
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Effect of cultivation methods on nutritional enrichment of euryhaline rotifer Brachionus plicatilis
KOTANI Tomonari, GENKA Teruhisa, FUSHIMI Hiroshi, HAYASHI Masahiro, DIERCKENS Kristof, SORGELOOS Patrick
Fisheries science : FS 75 ( 4 ) 975 - 984 2009.7
Language：Japanese Publishing type：Research paper (scientific journal)
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Influence of Culture Temperature on the Growth, Lipid Content and Fatty Acid Composition of Aurantiochytrium sp. Strain mh0186 Reviewed
Yousuke Taoka, Naoki Nagano, Yuji Okita, Hitoshi Izumida, Shinichi Sugimoto, Masahiro Hayashi
Marine Biotechnology 11 368 - 374 2009.6
Language：English Publishing type：Research paper (scientific journal)
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Extracellular enzymes produced by marine eukaryotes, thraustochytrids
Taoka Y., Nagano N., Okita Y., Izumida H., Sugimoto S., Hayashi M.
Bioscience, Biotechnology and Biochemistry 73 ( 1 ) 180 - 182 2009.2
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Bioscience, Biotechnology and Biochemistry
Extracellular enzymes produced by six strains of thraustochytrids, Thraustochytrium, Schizochytrium, and Aurantiochytrium, were investigated. These strains produced 5 to 8 kinds of the extracellular enzymes, depending on the species. Only the genus Thraustochytrium produced amylase. When insoluble cellulose was used as substrate, cellulase was not detected in the six strains of thraustochytrids. This study indicates that marine eukaryotes, thraustochytrids, produced a wide variety of extracellular enzymes.
DOI： 10.1271/bbb.80416
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Taoka Yousuke, Nagano Naoki, Okita Yuji, IZUMIDA Hitoshi, SUGIMOTO Shinichi, HAYASHI Masahiro
Journal of bioscience and bioengineering 105 ( 5 ) 562 - 565 2008.5
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：公益社団法人日本生物工学会
Tween 80, KH_2PO_4 and tomato juice were added to basal medium for the isolation of thraustochytrids. By the addition of Tween 80 and KH_2PO_4, the number of thraustochytrids isolated from seawater increased. KH_2PO_4 and Tween 80 were considered to be useful for isolating thraustochytrids.
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Kotani Tomonari, Genka Teruhisa, Fushimi Hiroshi, Hayashi Masahiro, Dierckens Kristof, Sorgeloos Patrick
福山大学内海生物資源研究所報告 18 11 - 12 2008.2
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：福山大学
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Miyamoto E., Tanioka Y., Yukino T., Hayashi M., Watanabe F., Nakano Y.
Journal of Nutritional Science and Vitaminology 53 ( 6 ) 471 - 475 2007.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of Nutritional Science and Vitaminology
A marine eukaryotic microorganism, Schizochytrium limacinum SR21, had the ability to absorb and accumulate exogenous cobalamin, which was converted to the cobalamin coenzymes 5′-deoxyadenosylcobalamin (20.1%) and methylcobalamin (29.6%). A considerably high activity (about 38 mU/mg protein) of 5′-deoxyadenosylcobalamin-dependent methylmalonyl-CoA mutase (EC 5.4.99.2) involved in amino acid and odd-chain fatty acid metabolism was found in the cell homogenate of S. limacinum SR21. The enzyme was purified to homogeneity and characterized.
DOI： 10.3177/jnsv.53.471
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Kotani Tomonari, Genka Teruhisa, Fushimi Hiroshi, Hayashi Masahiro
Annual report of Faculty of Life Science and Biotechnology, Fukuyama University 6 35 - 49 2007.9
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Fukuyama University
It is important to evaluate the effect of the primary cultivation method of rotifers on the secondary cultivation as nutritional enrichment. So far, various methods of rotifer cultivation have been developed. Recently, mainly two methods are performed, batch or continuous culture. This study aimed to clarify the fatty acid contents after the nutritional enrichment in order to evaluate the quality of rotifers cultured with different methods. Two primary rotifer cultures were performed using batch or continuous methods. From the batch culture, three experimental populations were used; they were taken from the culture one, 24 and 48 hour after inoculation of the rotifers. The continuous culture was performed with two tanks; one was for cultivation with continuous feeding and water supply (cultivation tank), and another was for stocking from cultivation tank by over flow (harvest tank). From the continuous culture, two experimental populations were used from the cultivation and harvest tanks. Secondary cultures were performed after each primary culture and each rotifer population was nutritionally enriched with Nannochloropsis oculata and a commercial nutritional enrichment diet. After secondary culture, the fatty acid contents of each population were analyzed using gas chromatography. Although there was no significant difference of lipid quantity among primary rotifer cultures, in both cases of secondary culture, total n-3 HUFA quantity from both continuous culture populations was higher than that from batch culture population one and 48 hour after inoculation. When the enrichment was performed with N. oculata, rotifer populations from the two tanks of continuous culture and the batch culture tank 24 hour after inoculation contained a higher quantity of ARA and EPA than those from the two other samples taken from the batch culture. When the enrichment was performed with enrichment diet, populations from the two tanks of continuous culture and the sample taken 24 hour after inoculation from the batch culture tank contained higher quantities of ARA, EPA and DHA than those from the two other samples taken from the batch culture.
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Effect of vitamin B12-enriched thraustochytrids on the population growth of rotifers
Hayashi M., Yukino T., Watanabe F., Miyamoto E., Nakano Y.
Bioscience, Biotechnology and Biochemistry 71 ( 1 ) 222 - 225 2007.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Bioscience, Biotechnology and Biochemistry
Newly isolated thraustochytrids showed uptake of vitamin B12 from the culture into the cells. Cultivation of thraustochytrids in a medium containing 1 μg/ml of vitamin B12 greatly increased the contents of vitamin B12 in the cells. Similarly to Schizochytrium limacinum, odd numbered fatty acids decreased in the cells of new isolates cultivated with vitamin B12. Vitamin B12-enriched thraustochytrids, strain mh0186, enhanced the population growth of rotifers fed on the cells as sole feed.
DOI： 10.1271/bbb.60308
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Rotifers fed with n-3 highly unsaturated fatty acid-enriched Chlorella vulgaris are suitable for the rearing of larval red sea bream Pagrus major. Reviewed
Maruyama, I., Yamamoto, S., Hayashi, M., Murata, O.
Aquaculture Science 54 229 - 230 2006.11
Language：English Publishing type：Research paper (scientific journal)
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Hemolytic suppression roles of taurine in yellowtail Seriola quinqueradiata fed non-fishmeal diet based on soybean protein
Takagi, S., Murata, H., Goto, T., Hayashi, M., Hatate, H., Endo, M., Yamashita, M., Ukawa, M.
Fisheries Science 72 546 - 555 2006.10
Language：Japanese Publishing type：Research paper (scientific journal)
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Abe Eriko, Hayashi Yasuhiro, Hama Yoichiro, HAYASHI Masahiro, INAGAKI Masanori, ITO Makoto
The journal of biochemistry 140 ( 2 ) 247 - 253 2006.8
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Biochemical Society
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n-3高度不飽和脂肪酸強化クロレラChlorella vulgarisで培養したシオミズツボワムシによるマダイPagrus major仔魚の飼育
MARUYAMA Isao, YAMAMOTO Shinji, HAYASHI Masahiro, MURATA Osamu
水産増殖 54 ( 2 ) 229 - 230 2006.6
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：水産増殖談話会
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MARUYAMA Isao, YAMAMOTO Shinji, HAYASHI Masahiro, MURATA Osamu
水産増殖 54 ( 2 ) 229 - 230 2006
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：日本水産増殖学会
Rearing of larval red sea bream <I>Pagrus major</I> was performed by feeding with rotifers cultured in <I>Chlorella vulgaris</I> K-22 enriched with n-3HUFAs (mainly DHA) . Results for larval growth, survival rate, activity, and air-bladder opening rate suggested the successful rearing of the red sea bream. The total content of n-3HUFAs in the larvae at the end of the rearing was even higher than the level immediately after hatching. It can therefore be concluded that the enhanced n-3HUFAs in <I>C. vulgaris</I> were effective via rotifer for the rearing of red sea bream larvae. The n-3HUFA-enriched <I>C. vulgaris</I> makes it possible to realize the rearing of red sea bream without a secondary rotiferculture process for enhancement of n-3HUFAs.
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Effect of dietary laurate on lipid accumulation and vitality in juvenile Pagrus major
共著者：Hong, J., Om, A. D., Umino, T., Yoshimatsu, T., Hayashi, M., Nakagawa, H., Asano, M., Nakagawa, A.
Journal of Fisheries of ChinaVol. 29, 804-810 2005.12
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2・3 ワムシの栄養強化方法(2.集中課題「ワムシ-その生物学、培養法から餌料価値-」,第三回因島種苗生産技術交流会,学会講演要旨)
林 雅弘
福山大学内海生物資源研究所報告 16 25 - 26 2005.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：福山大学