Papers - KUNITAKE Hisato
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Kunitake H., Kagami H., Mii M.
Scientia Horticulturae 47 ( 1-2 ) 27 - 33 1991.6
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Scientia Horticulturae
Protoplasts were isolated from embryogenic calli of 'Satsuma' mandarin (Citrus unshiu Marc. cultivar 'Tokumori Wase') and cultured in Gellan gum-embedded Murashige and Tucker (MT) medium containing 40 mg l -1 adenine, 0.15 M sucrose and 0.45 M mannitol. The percentage of colony formation (plating efficiency) 40 days after culture was 46%, much higher than that in MT medium without adenine. Most of the colonies grew about 1 mm after 2 months of culture and were successfully transferred onto MT medium containing 5% lactose for embryoid formation. Five percent of the resulting embryoids germinated normally and grew into plantlets, 30-60 days after transplanting on MT medium containing 1 mg l -1 gibberellic acid, 3% sucrose and 0.2% Gellan gum. Plant regeneration from protoplasts was also obtained in other important selections, cultivars 'Miyagawa Wase' and 'Okitsu Wase', using the same methods. © 1991.
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Somatic embryogenesis and plant regeneration from protoplasts of ‘satsuma’mandarin (Citrus unshiu Marc.).
共著者：Kunitake, H., H. Kagami , M. Mii
Scientia HorticulturaeVol. 47, No. 1-2, 27-33 1991.2
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Plant regeneration from cell culture-derived protoplasts of statice (Limonium perezii). Reviewed
共著者：Kunitake, H. M. Mii
Plant Science 70 ( 1 ) 115 - 119 1990.11
Language：English Publishing type：Research paper (scientific journal)
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Plant regeneration from protoplasts isolated from embryogenic callus of satsuma(Citrus unshiu Marc.) Reviewed
共著者：Ling, J.T., N. Nito,M. Iwamasa, H. Kunitake
HortScience 25 ( 8 ) 970 - 972 1990.8
Language：English Publishing type：Research paper (scientific journal)
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Somatic embryogenesis and plant regeneration from protoplast of asparagus (Asparagus officinalis L.). Reviewed
共著者：Kunitake, H. M. Mii
Plant Cell Reports 8 ( 12 ) 706 - 710 1990.8
Language：English Publishing type：Research paper (scientific journal)
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Kunitake H., Mii M.
Plant Cell Reports 8 ( 12 ) 706 - 710 1990.5
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Plant Cell Reports
Protoplasts were isolated from embryogenic calli of Asparagus officinalis L. cv. Mary Washington and cultured in 1/2 MS medium with 1 mg/l NAA, 0.5 mg/l zeatin, 1 g/l L-glutamine, 0.6 M glucose and 0.1% Gellan Gum. Protoplasts started to divide after 3-4 d of culture and formed visible colonies after 30 d of culture. The percentage of colony formation (plating efficiency) was 7.2%. The colonies were then transferred onto Gellan Gum-solidified MS medium containing 1 mg/l 2,4-D and 3% sucrose for further growth. Somatic embryos were induced from all colonies of 0.5-1.0 mm size after transferring to 1/2 MS medium lacking growth regulators. After treating these somatic embryos (1-3 mm) in distilled water for a week, 30-40% of them germinated normally and grew into plantlets 20-30 d after transplanting on 1/2 MS medium containing 1 mg/l IBA, 1 mg/l GA 3 and 1% sucrose. These protoplast-derived plants were diploid with 20 chromosomes. © 1990 Springer-Verlag.
DOI： 10.1007/BF00272099
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Plant regeneration from cell culture-derived protoplasts of statice (Limonium perezii Hubbard)
Kunitake H., Mii M.
Plant Science 70 ( 1 ) 115 - 119 1990
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Plant Science
Protoplasts were isolated from leaf segment-derived suspension cultures of Limonium perezii Hubbard and cultured in Gellan Gum-solidified 1 2 Murashige and Skoog (MS) medium containing 1 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), 1 mg/l naphthaleneacetic acid (NAA), 1 mg/l 6-benzylaminopurine (BA), 250 mg/l casein hydrolysate, 3% (w/v) sucrose and 0.5 M mannitol. The protoplasts started to divide within 2-3 days of culture and formed colonies (0.5-1 mm) after 2 months of culture. These colonies were then transferred to MS medium containing 1 mg/l 2,4-D, 3% sucrose and 0.2% (w/v) Gellan Gum for callus proliferation. The calli were then transferred for shoot regeneration to MS medium with or without 2 mg/l zeatin. These shoots were rooted on MS medium containing 0.5 mg/l indole-3-butyric acid (IBA), 3% sucrose and 0.2% Gellan Gum and transferred to pots. No abnormalities in leaf shape and growth habit were observed in these plants. © 1990.