Papers - KUNITAKE Hisato
-
Somaclonal and chromosomal effects of genotype, ploidy and culture duration in Asparagus officinalis L. Reviewed
共著者: Kunitake, H., T. Nakashima, K. Mori , M. Tanaka
Euphytica 102 ( 3 ) 309 - 316 1998.7
Language：English Publishing type：Research paper (scientific journal)
-
Somatic embryogenesis and its application for breeding and micropropagation in asparagus (Asparagus officinalis L.). Reviewed
共著者: Kunitake, H. , M. Mii
Plant Biotechnology 15 ( 2 ) 51 - 61 1998.6
Language：English Publishing type：Research paper (scientific journal)
-
Kunitake H., Nakashima T., Mori K., Tanaka M.
Euphytica 102 ( 3 ) 309 - 316 1998
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Euphytica
Chromosome and morphological variations of embryogenic calli-derived plants of gynogenic haploid, diploid, triploid and tetraploid asparagus (Asparagus officinalis L.) were investigated. Artificial tetraploids were produced using colchicine treatment of seeds of diploid cultivar, 'Poultom'. 'Haidel' (2X) was crossed with the artificial tetraploids, from which one gynogenic haploid, one diploid, 6 triploid, 3 mixoploids were obtained. Embryogenic calli were first obtained from crown buds, subsequently induced to form somatic embryos, and after 30 days, induced to germinate. Chromosome variation in embryogenic calli-derived plants increased with increasing duration of subculture, particularly when low ploidy levels of plants such as haploid and diploid were used as explants. Approximately 80% of haploid-derived plants showed morphological variations such as dwarfness and abnormal morphological characteristics, although no differences were observed in cladodes and stem characteristics between other polyploid-derived plants and their parents. The data presented here would supply important fundamental information for commercial mass-propagation using somatic embryogenesis.
-
Kunitake H., Masahiro M.
Plant Biotechnology 15 ( 2 ) 51 - 61 1998
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Plant Biotechnology
-
Normalization of asparagus somatic embryogenesis using a maltose-containing medium. Reviewed
共著者: Kunitake, H., T. Nakashima, K. Mori , M. Tanaka
Journal of Plant Physiology 150 ( 4 ) 458 - 461 1997.4
Language：English Publishing type：Research paper (scientific journal)
-
Normalization of asparagus somatic embryogenesis using a maltose-containing medium
Kunitake H., Nakashima T., Mori K., Tanaka M.
Journal of Plant Physiology 150 ( 4 ) 458 - 461 1997
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of Plant Physiology
Embryogenic calli derived from internal buds were cultured and maintained on Murashige and Skoog (MS) medium containing 2 mg/L 2,4-D, 3% sucrose and 0.2% gellan gum. Normal somatic embryos were induced from the embryogenic calli by pretreating with 1/4 MS liquid medium for 7 days, sieving through 600 μm stainless steel mesh and then partially desiccating on plant growth regulator-free MS medium containing a high concentration of gellan gum (1.0% w/v) for 1 week. Furthermore, use of maltose as a sugar and/or osmoticum in the embryo induction medium promoted development of normal somatic embryos. Using these methods, non-vitrified bipolor embryos were induced 30 days after transfer (approximately 1,500 embryos per 0.1 mL packed cell volume). The somatic embryos induced on maltose-containing medium exhibited a much higher germination rate more than 80%) with cold treatment (14 days at 4°C) than that induced on sucrose-containing medium.
-
Polyacetylenes in hairly root cultures of Campanula medium L. Reviewed
共著者: Toda, H., T. Nakashima, H. Kunitake, K. Mori, M. Tanaka , K. Ishimaru
Journal of Plant Physiology 147 ( 5 ) 617 - 619 1996.12
Language：English Publishing type：Research paper (scientific journal)
-
Production of interspecific somatic hybrid plants between Asparagus officina- lis and A. macowanii through electrofusion. Reviewed
共著者: Kunitake, H., T. Nakashima, K. Mori, M. Tanaka, A. Saito , M. Mii
Plant Science 116 ( 2 ) 213 - 222 1996.6
Language：English Publishing type：Research paper (scientific journal)
-
Polyacetylenes in hairy root cultures of Campanula medium L.
Tada H., Nakashima T., Kunitake H., Mori K., Tanaka M., Ishimaru K.
Journal of Plant Physiology 147 ( 5 ) 617 - 619 1996.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of Plant Physiology
Campanula medium L. hairy roots induced by infection with Agrobacterium rhizogenes A 13 grew well in three hormone-free liquid media, Murashige-Skoog (MS), Gamborg B5 (B5) and Woody Plant (WP). In these media, the hairy roots produced a high amount of the polyacetylene monoglucoside, lobetyolin (maximum content: 3.74% in MS medium, 2.69% in B5 medium and 3.13% in WP medium, dry weight) whose levels were over 130 times higher than that of the intact plant.
-
Kunitake H., Nakashima T., Mori K., Tanaka M., Saito A., Mii M.
Plant Science 116 ( 2 ) 213 - 222 1996
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Plant Science
Interspecific somatic hybridization was performed between embryogenic callus protoplasts of Asparagus officinalis cv. Mary Washington and callus protoplasts of A. macowanii by electrofusion. By utilizing iodoacetamiae (IGA) inactivation treatment for A. officinalis protoplasts and the difference of cell division capacity between the two species, one callus line, which had an ability to produce somatic embryos, was selected as a putative hybrid. Normal germination of the somatic embryos was induced only when they were exposed to a period of cold treatment (14 days at 4°C). However, the regenerated plants showed intermediate but abnormal morphological characters in vitro as well as after transfer to soil. The hybrid nature of the plants derived from this callus was confirmed by isozyme, nuclear rDNA and random amplified polymorphic DNA (RAPD) analyses and counting the chromosome number. © 1996 Elsevier Science Ireland Ltd. All rights reserved.
-
Plant regeneration from mesophyll protoplasts of lisianthus (Eustoma grandi- florum) by adding activated charcoal into protoplast culture medium. Reviewed
共著者: Kunitake, H., T. Nakashima, K. Mori, M. Tanaka , M. Mii
Plant Cell, Tissue and Organ Culture 43 ( 1 ) 59 - 65 1995.12
Language：English Publishing type：Research paper (scientific journal)
-
Polyphenol production in hairly root cultures of Fragaria x ananassa. Reviewed
共著者: Motomori, Y., K. Shimomura, K. Mori, H. Kunitake, T. Nakashima, M. Tanaka, S. Miyazaki , K. Ishimaru
Phytochemistry 40 ( 5 ) 1425 - 1428 1995.12
Language：English Publishing type：Research paper (scientific journal)
-
Polyphenol production in hairy root cultures of Fragaria x ananassa
Motomori Y., Shimomura K., Mori K., Kunitake H., Nakashima T., Tanaka M., Miyazaki S., Ishimaru K.
Phytochemistry 40 ( 5 ) 1425 - 1428 1995.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Phytochemistry
Hairy roots of Fragaria × ananassa cv. Reikou, induced with Agrobacterium rhizogenes ATCC 15834, grew well in hormone-free Murashige-Skoog (MS), root culture and Gamborg B5 liquid media. Particularly, in MS medium, hairy roots whowed maximum growth (539 mg per flask, dry wt at week 8) producing high contents of polyphenols (especially (+)-catechin (0.59% dry wt at week 8) and procyanidin B-3 (0.80% dry wt at week 7). Polyphenol contents in the intact plant (leaf blade, petiole, calyx, receptacle and root) were also investigated. © 1995.
-
Kunitake H., Nakashima T., Mori K., Tanaka M., Mii M.
Plant Cell, Tissue and Organ Culture 43 ( 1 ) 59 - 65 1995.10
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Plant Cell, Tissue and Organ Culture
Plant regeneration from isolated protoplasts of 8 cultivars of lisianthus, Eustoma grandiflorum (Griseb.) Schinners, has been established by using activated charcoal. Protoplasts were isolated from lisianthus leaves grown in vitro and started to divide within 3-4 days of culture, but successful colony formation was only achieved by adding gellan gum blocks containing 1% (w/v) activated charcoal immediately after culture. Colonies consisting of as many as 50-100 cells formed after 30 days of culture and were transferred to fresh medium for callus proliferation and shoot regeneration, respectively. These shoots rooted on MS medium containing 0.5 mg l -1 indolebutyric acid(IBA) and the plantlets were finally transplanted to pots. Morphological characteristics, growth habit and pollen fertility of protoplast-derived plants of one cultivar were not different from those of seed-grown plants as control. © 1995 Kluwer Academic Publishers.
DOI： 10.1007/BF00042672
-
Morphological and cytological characteristics of protoplast-derived plants of statice (Limonium perezii Hubbard).
共著者：Kunitake, H., K. Koreeda , M. Mii
Scientia Horticulturae 60 ( 3-4 ) 305 - 312 1995.3
Language：English Publishing type：Research paper (scientific journal)
-
Kunitake H., Koreeda K., Mii M.
Scientia Horticulturae 60 ( 3-4 ) 305 - 312 1995.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Scientia Horticulturae
Morphological characteristics of 56 protoplast-derived plants (protoclones) of statice (Limonium perezii Hubbard) were compared with meristem-derived plants (controls). Some protoclones had smaller leaf shape index (length/width) and shorter length of flower stalk and calyx than the controls. However, no distinct somaclonal variations were observed in other characteristics such as color of calyx and number of petals and stamens, except for protoclone No. 1-7, which had flowers with abnormal petals and low pollen fertility (25%). Microscopic observation showed that all of the 17 protoclones examined, including clone No. 1-7, had the normal diploid chromosome number of 14. © 1995.
-
Isolation and culture of asparagus microspore protoplasts. Reviewed
共著者：Kunitake, H., T. Godo , M. Mii
Japanese Journal of Breeding 43 ( 2 ) 231 - 238 1993.6
Language：English Publishing type：Research paper (scientific journal)
-
Somatic embryogenesis and plant regeneration from immature seed-derived calli of rugosa rose (Rosa rugosa Thunb.). Reviewed
共著者：Kunitake, H., H. Imamizo , M. Mii
Plant Science 90 ( 2 ) 187 - 194 1993.4
Language：English Publishing type：Research paper (scientific journal)
-
Kunitake H., Imamizo H., Mii M.
Plant Science 90 ( 2 ) 187 - 194 1993
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Plant Science
A procedure for plant regeneration from immature seed-derived calli of rugosa rose (Rosa rugosa Thunb.) via somatic embryogenesis is described. Embryogenic calli were initiated from immature seeds 2-3 weeks after anthesis on Murashige and Skoog (MS) medium without growth regulators. Induced calli had a white, friable and nodular appearance with several proembryos. These calli were subcultured at 20-day intervals on MS medium containing 0.1-0.2 M galactose on which they grew rapidly; but somatic embryogenesis was inhibited. Somatic embryos were again induced from the subcultured calli after transferring to MS medium containing 0.1 M M fructose or sucrose but lacking growth regulators. After transferring these embryos (1-2 mm) to MS medium containing 0.1 M sorbitol, 3% of them germinated and grew into plantlets which showed sustained growth on the MS medium containing only 0.1 M sorbitol as the sole carbon source. © 1993.
-
Plant regeneration from protoplasts of ‘satsuma’mandarin (Citrus unshiu Marc.). Reviewed
共著者：Kunitake, H., H. Kagami , M. Mii
Japan Agricultural Research QuarterlyVol. 24, No. 4, 287-291 24 ( 4 ) 287 - 291 1991.7
Language：English Publishing type：Research paper (scientific journal)