Papers - KUNITAKE Hisato
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Yahata Masaki, Nukaya Tsunaki, Sudo Miki, Ohta Tomohiro, Yasuda Kiichi, Inagaki Hidehiro, Mukai Hiroo, Harada HIsashi, Takagi Toshihiro, Komatsu Haruki, Kunitake Hisato
The Horticulture Journal 84 ( 1 ) 30 - 36 2015.2
Language：English Publishing type：Research paper (scientific journal)
DOI： 10.2503/hortj.MI-005
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Functional genetic analysis of Arabidopsis thariana SYNC1 in Lotus corniculatus super-growing roots using the FOX gene-hunting system. Invited Reviewed
Yano Tsubasa, Tanaka Hidenori, Kurino Taiki, Yamamoto Akihiro, Kunitake Hisato, Saeki Yuichi, Akashi Ryo
Plant Root 9 6 - 14 2015.2
Language：English Publishing type：Research paper (scientific journal)
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Abe A., Uchida A., Hoshino Y., Sakakibara Y., Suiko M., Kunitake H.
Acta Horticulturae 1065 1267 - 1274 2015
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Acta Horticulturae
To analyze the protein expression of pollen grains and tubes having self-incompatible reactions in Hyuganatsu (Citrus tamurana Hort. ex Tanaka), we cultured mature pollen and treated it with crude extracts from the styles using a mass or single liquid culture system, and we collected pollen grains and tubes for proteome analysis. The relative expression of each identified protein was quantified by an imager and revealed as a value relative to the pollen grain. Eleven identified proteins were remarkably up-regulated (above 1.2-fold) or down-regulated (under 0.8-fold) in treatment with self-incompatible reactions. Eight of these proteins were predicted to be self-incompatible-related proteins with the reported functions. In this study, F-box protein was identified as showing minimum expression in the treatment with crude extracts from styles of Hyuganatsu (self-incompatible reactions). The S-locus gene product expressed in pollen has been identified as an F-box protein in Solanaceae and Rosaceae. It is not clear whether a mechanism similar to that of Solanaceae and Rosaceae exists in the self-incompatible reaction in Hyuganatsu; however, the reduced expression of F-box protein may induce a self-incompatible reaction.
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Yasuda Kiichi, Yahata Masaki, Kunitake Hisato
The Horticulture Journal 0 ( 0 ) 2015
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society for Horticultural Science
Kumquats (<i>Fortunella</i> spp.) is classified into the subfamily Aurantioideae (family Rutaceae). The taxonomy and phylogeny of this genus are complicated and controversial. Therefore, we carried out a estimation based on chromomycin A<sub>3</sub> (CMA) karyotype composition in order to understand the cytogenetics and evolution of the genus <i>Fortunella</i>. Among the 6 <i>Fortunella</i> species examined, <i>Fortunella hindsii</i> var. <i>chintou</i> Swing. (Hongkong kumquat) showed the simplest CMA karyotype composition. On the other hand, close relationships were found among the 3 species <i>F. margarita</i> (Lour.) Swing. (Oval kumquat), <i>F. japonica</i> (Thunb.) Swing. (Round kumquat), <i>F. crassifolia</i> Swing. (Meiwa kumquat). <i>Fortunella polyandra</i> (Ridl.) Tan. (Malayan kumquat) and <i>F. obovata</i> hort. ex Tan. (Changshou kumquat) had type E chromosomes, which is an elemental chromosome type for <i>Citrus</i>. We concluded that there are only two true species for the genus <i>Fortunella</i>, <i>F. hindsii</i> and <i>F. margarita</i> complex, which includes <i>F. margarita</i>, <i>F. japonica</i>, and <i>F. crassifolia</i>, and that <i>F. polyandra</i> and <i>F. obovata</i> should be classified as natural or horticultural hybrids.
DOI： 10.2503/hortj.MI-078
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Li Y., Uchida A., Abe A., Yamamoto A., Hirano T., Kunitake H.
Journal of the American Society for Horticultural Science 140 ( 2 ) 183 - 190 2015
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of the American Society for Horticultural Science
© 2015, American Society for Horticultural Science. All rights reserved. Citrus is a genus with gametophytic self-incompatibility, which prevents fertilization by self-pollination. Polyamines (PAs), as a class of active small molecules, widely participate in various cellular activities. To investigate the effects of the changes in PA contents [putrescine, spermidine (SPD), and spermine] on pollen tube growth in self-incompatible (SI) plants, we cultured pollen of Citrus maxima ‘Banpeiyu’ and C. tamurana ‘Hyuganatsu’ in a Citrus mature pollen culture system and detected the PA content changes in pollen by high-performance liquid chromatography (HPLC) analysis during germination and after treatment with crude protein extracts of compatible or incompatible styles. We profiled the PA content changes during the germination course in pollen of ‘Banpeiyu’ and ‘Hyuganatsu’ by HPLC, and identified increases of free and perchloric-acid-insoluble SPD contents in pollen tubes in the incompatible treatment. Exogenous SPD treatment obviously increased the SPD contents in the pollen tube, inhibited pollen tube elongation, and resulted in some abnormal morphological alterations, such as tip inflation, tube content leakage and tip twist, which were observed in both ‘Banpeiyu’ and ‘Hyuganatsu’ pollen tubes exposed to incompatible treatment. This finding implied that SPD might participate in the SI response in Citrus.
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クロマメノキとラビットアイブルーベリーT100との節間交雑から得られたF1系統の評価 Reviewed
執行 みさと、森田 恭代、西村 謙一、井上 論司、國武 久登、小松 春喜
園芸学研究 13 ( 4 ) 323 - 333 2014.10
Language：Japanese Publishing type：Research paper (scientific journal)
DOI： 10.2503/hrj.13.323
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The phenomenon of root elongation and high respiration actibity in the rolB-gene-enhanced FSL#35 variant of Lotus corniculatus FOX-SR line. Reviewed
Yano Tsubasa, Yamamoto Akihiro, Kunitake Hisato, Saeki Yuichi, Akashi Ryo
Plant Root 8 82 - 91 2014.8
Language：English Publishing type：Research paper (scientific journal)
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我が国自生スノキ属野生種クロマメノキとハイブッシュブルーベリー‘ブルークロップ’との節間交雑から得られたF1系統の評価 Reviewed
執行みさと、具志堅 文、桂川明広、臂 光昭、吉岡克則、鹿毛哲郎、國武久登、小松春喜
園芸学研究 13 ( 2 ) 97 - 106 2014.6
Language：Japanese Publishing type：Research paper (scientific journal)
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Comparison of Cultivars and Seasonal Variation in Blueberry (<i>Vaccinium</i> Species) Leaf Extract on Adult T-Cell Leukemia Cell Line Growth Suppression.
Kai H, Fuse T, Kunitake H, Morishita K, Matsuno K
Medicines (Basel, Switzerland) 1 ( 1 ) 3 - 11 2014.6
Language：Japanese Publishing type：Research paper (scientific journal)
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我が国自生スノキ属野生種クロマメノキとハイブッシュブルーベリー ブルークロップとの節間交雑から得られたF1系統の評価 Reviewed
執行 みさと、具志堅 文、桂川 明広、臂 光昭、吉岡 克則、鹿毛 哲朗、國武 久登、小松 春喜
園芸学研究 13 ( 2 ) 97 - 106 2014.4
Language：Japanese Publishing type：Research paper (scientific journal)
DOI： 10.2503/hrj.13.97
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ブルーベリー近縁種ナツハゼとアラゲナツハゼにおける果実の成熟特性と品質評価 Reviewed
津田浩利, 小島祥子, 大坪早貴, 小松春喜, 國武久登
園芸学研究 13 ( 1 ) 1 - 9 2014.3
Language：Japanese Publishing type：Research paper (scientific journal)
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Efficient in vitro screening for higher soil pH adaptability of intersectional hybrids in blueberry
Tsuda H., Kunitake H., Aoki Y., Oyama A., Tetsumura T., Komatsu H., Yoshioka K.
HortScience 49 ( 2 ) 141 - 144 2014.2
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：HortScience
We tested efficient in vitro methods for screening the genotypes with higher pH tolerance using multiple shoots of intersectional hybrids between Vaccinium corymbosum 'Spartan' and V. bracteatum. The response of the four hybrid clones tested to different pH levels was clone-dependent in vitro. An apparent difference was found in the rooting rate among the hybrid clones even at higher pH levels; the rooting rates of JM4 (91%) at pH 8.0 indicated a significantly high value compared with other clones (JM1: 24%, JM2: 9%, JM3: 8%, 'Spartan': 0%). Furthermore, JM4 showed constantly high rooting rates (91% to 100%) at all pH levels with no significant differences. Similar differences in the root characters of the hybrids were also confirmed by checking the viability of roots using fluorescein diacetate (FDA)/propidium iodide (PI) staining after dipping the roots of in vitro-produced shoots in liquid medium at different pH levels for 6 hours. These results suggest that an in vitro screening method using the rooting rate of multiple shoots and the viability test of roots by FDA/PI staining as a marker could become a very useful tool for the selection of germplasm with tolerance to higher pH within a short time using small planting spaces. In addition, JM4, which showed a high rooting rate at pH 8.0, could be useful in breeding new cultivars with higher pH tolerance.
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Tsuda Hirotoshi, Kojima Shoko, Otsubo Saki, Komatsu Haruki, Kunitake Hisato
Hort. Res. (Japan) 13 ( 1 ) 1 - 9 2014
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
To obtain fundamental data for using Natsuhaze and Aragenatsuhaze in the breeding and cultivation of blueberries, we examined the fruit growth characteristics and fruit components. Natsuhaze and Aragenatsuhaze had many fruits per fruit cluster compared with blueberry cultivars, and the fruit clusters were long and slender. Although the maturation period of the fruits within the same fruit cluster was different, the matured fruits did not fall for a long period. Therefore, this species may be harvested by cluster harvesting, as with grapes. The maturation periods of both Natsuhaze and Aragenatsuhaze were from September to November, one to three months later than the latest-maturing blueberry cultivars. Although the sugar-acid ratio in the fruits of Natsuhaze and Aragenatsuhaze was lower than in blueberry cultivars and the taste was inferior, the total anthocyanin content of fruits in Natsuhaze was about 6 times that of blueberries. The total polyphenol content, total proanthocyanidin content, and antioxidant activities showed high values in unripe fruits, and increased as maturity progressed in these species. Mature fruit of Aragenatsuhaze had 3 times more total polyphenols and 17 times more total proanthocyanidin than blueberry cultivars; it was suggested that total polyphenols including proanthocyanidin contribute to antioxidant activities. Natsuhaze and Aragenatsuhaze could be useful breeding materials for new cultivar production by expanding the crop season of blueberries, enabling cluster harvesting, and offering high biological functionality.
DOI： 10.2503/hrj.13.1
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Evaluation of Some Wild Species of Rubus Native to Japan as Breeding Materials. Reviewed
12 ( 4 ) 335 - 342 2013.12
Language：Japanese Publishing type：Research paper (scientific journal)
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Tsuda H., Kunitake H., Yamasaki M., Komatsu H., Yoshioka K.
Journal of the American Society for Horticultural Science 138 ( 4 ) 317 - 324 2013.7
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of the American Society for Horticultural Science
With crosses between colchicine-induced tetraploid shashanbo (Vacciniumbracteatumsection Bracteata) and tetraploid highbush blueberry 'Spartan' (Vaccinium corymbosum section Cyanococcus), intersectional hybrids were produced. The hybridity of these plants was confirmed based on DNA markers and morphological characteristics. The morphological characteristics, blooming date, and ripening period of the hybridswere intermediate between those of the parents. Ploidy analyses by flow cytometry and chromosome counting revealed that these hybrids were tetraploid. Four hybrids set fruit in the field and these two hybrids showed high pollen stainability. It was noteworthy that fruit of two hybrids had high soluble solids concentration compared with 'Spartan' and the fruit pulp of the hybrids was tinged with red as shashanbo. These hybrids could be useful in breeding new cultivars with high sugar content, abundant phytochemicals, extensive environmental adaptability as well as late flowering and fruit maturity.
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Tsuda, H., Kunitake, H., Ryoko Kawasaki-Takaki, R., Nishiyama, K., Yamasaki, M., Komatsu, H., Yukizaki
Plants 2 ( 1 ) 57 - 71 2013.3
Language：English Publishing type：Research paper (scientific journal)
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Antioxidant Activities and Anti-Cancer Cell Proliferation Properties of Natsuhaze (Vaccinium oldhamii Miq.), Shashanbo (V. bracteatum Thunb.) and Blueberry Cultivars.
Tsuda H, Kunitake H, Kawasaki-Takaki R, Nishiyama K, Yamasaki M, Komatsu H, Yukizaki C
Plants (Basel, Switzerland) 2 ( 1 ) 57 - 71 2013.2
Language：Japanese Publishing type：Research paper (scientific journal)
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Evaluation of Some Wild Species of <i>Rubus</i> Native to Japan as Breeding Materials
Shigyo Misato, Chikuma Kouhei, Kunitake Hisato, Komatsu Haruki
Hort. Res. (Japan) 12 ( 4 ) 335 - 342 2013
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
There is increasing interest in fruits of bramble crops (<i>Rubus</i> spp.) because of the possible benefical health effects associated with their phenolic content. Almost all cultivars are from Europe and the United States, although there are many wild <i>Rubus</i> species native to Japan. In order to obtain basic knowledge for the horticultural application of those wild species, we collected and evaluated some wild species native to Kyusyu Island as genetic resources. All raspberry cultivars were diploid, but almost all wild species were diploid with a different genome size, except for a few hexaploids. The aggregate fruit weights of wild species were not smaller than cultivars except for <i>Rubus lambertianus</i> Seringe. The ripening fruits of some wild species, <i>R. crataegifolius</i> Bubge., <i>R. trifidus</i> Thunb., and <i>R. palmatus</i> Thunb., were higher than the fruits of raspberry cultivars regarding total sugar contents and lower in total organic acid contents. The fruits of <i>R. crataegifolius</i> Bubge., <i>R. palmatus</i> Thunb., and <i>R. lambertianus</i> Seringe also showed a higher total polyphenol content and antioxidant activity than the cultivars. Some wild species native to Japan may be useful as breeding material to establish some new cultivars adaptable to Japan with high quality and functionality.<br>
DOI： 10.2503/hrj.12.335
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Effective micropropagation of rabbiteye blueberries for leaf tea production
Tetsumura T., Kajiwara Y., Honsho C., Sato-Yamauchi M., Sugimoto Y., Kunitake H.
Environmental Control in Biology 50 ( 3 ) 289 - 296 2012.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Environmental Control in Biology
Micropropagation of two clones of rabbiteye blueberry (Vaccinium virgatum Aiton) developed for leaf tea production was investigated for supply in high quantities to plantations and plant factories. Shoots of a new cultivar, 'Kunisato 35 Gou', were multiplied efficiently on MW, a mixture of equal parts of MS and WPM, supplemented with 20 μM zeatin by using 2-node segments. For No. 19, another promising clone, 5-node segments were the most productive for shoot multiplication. Among basal media tested for micropropagation, MW medium was found to be the most efficient. Although shoots of 'Kunisato 35 Gou' grew better in vitro than those of No. 19, both clones had equivalent rooting abilities. More than 90% of the shoots rooted and survived in vermiculite after culturing in Gellan gum-solidified rooting medium without plant growth regulators. The growth of shoots with roots in pots was significantly influenced by the potting soil. The plantlets in the soils mixed with peat moss, which made the soil acid, grew vigorously, while those in the soils without peat moss grew moderately or poorly, depending on soil type.
DOI： 10.2525/ecb.50.289
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Identification of self-incompatibility related proteins in the pistil of Japanese pear (Pyrus pyrifolia (Burm.f.)) by proteome analysis. Reviewed
1 Uchida, U., H. Sassa, S. Takenaka, Y. Sakakibara, M. Suiko, H. Kunitake
Plant Omics Journal ５ ( ４ ) 320 - 325 2012.8
Language：English Publishing type：Research paper (scientific journal)