Papers - KUNITAKE Hisato
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Efficient production of polyploid plants via protoplast culture of Iris fulva. Reviewed
共著者：Inoue, K., T. Kato, H. Kunitake , T. Yabuya
Cytologia 69 ( 3 ) 327 - 333 2004.9
Language：English Publishing type：Research paper (scientific journal)
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Efficient production of polyploid plants via protoplast culture of Iris fulva
Inoue K., Kato T., Kunitake H., Yabuya T.
Cytologia 69 ( 3 ) 327 - 333 2004.9
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Cytologia
In protoplast culture of the Louisiana iris species, Iris fulva (2n=42), N6 medium supplemented with 1 mg/l 2,4-D, 1 mg/l kinetin, 200 mg/l casein hydrolysate, 250 mg/l proline, 0.3 M glucose and 2.5 g/l gellan gum was suitable for cell division and colony formation. When colonies formed were transferred to plant growth regulator free MS medium, 61 plant lines were regenerated via somatic embryogenesis. Flow cytometric analysis revealed that leaf tissue of I. fulva consisted of 2C, 4C and 6C cells. Consequently, we identified this species as polysomatic in the genus Iris. Such polysomaty was also recognized in the suspension calli. Sixty one plant lines obtained by protoplast culture consisted of 25 diploid (41.0%), 1 triploid (1.6%), 29 tetraploid (47.5%) and 6 hexaploid lines (9.9%). In addition, chromosome analysis showed that somatic chromosome numbers of the diploid, hypertriploid and tetraploid lines were 2n=42, 67 and 84, respectively. On the other hand, all lines regenerated from suspension culture of I. fulva were diploid lines in contrast to the results from protoplast culture. Thus, it can be concluded that protoplast culture of this species is an efficient technique for production of polyploid plants.
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共著者：Takami, K., A. Matsumaru, M. Yahata, H. Kunitake , H. Komatsu
Plant Cell Reports 23 ( 1 ) 39 - 45 2004.8
Language：English Publishing type：Research paper (scientific journal)
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Takami K., Matsumara A., Yahata M., Imayama T., Kunitake H., Komatsu H.
Plant Cell Reports 23 ( 1-2 ) 39 - 45 2004.8
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Plant Cell Reports
Intergeneric somatic hybrids between embryogenic callus-derived protoplasts of round kumquat (Fortunella japonica Swingle) and 'Morita navel' orange (Citrus sinensis Osbeck) were produced by electrofusion. Among the eight different fusion strains obtained, six showed normal morphology, whereas the remaining two showed malformation. All the regenerated plants were intermediate in leaf morphology and had thick and round leaves, which are typical characteristics of polyploids. Ploidy analyses by flow cytometry and chromosome counting in root-tip cells revealed that these plants are amphidiploid (2n=4x=36). Hybridity of the fusion products was confirmed by random amplified polymorphic DNA and cleaved amplified polymorphic sequence (CAPS) analyses. Furthermore, analyses of chloroplast (cp) and mitochondrial (mt) DNA by CAPS showed that these somatic hybrids contained cp- and mt-DNA of round kumquat without recombination in the regions analyzed. © Springer-Verlag 2004.
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Yahata, M., Y. Kashihara, H. Kunitake, H. Komatsu
Horticultural Research 3 ( 1 ) 11 - 16 2004.4
Language：English Publishing type：Research paper (scientific journal)
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Yahata Masaki, Kashihara Yukiko, Kurogi Hironori, Kunitake Hisato, Komatsu Haruki
Hort. Res. (Japan) 3 ( 1 ) 11 - 16 2004
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
In vitro chromosome doubling of nucellar embryos was investigated in Meiwa kumquat (Fortunella crassifolia Swingle). Nucellar embryos were aseptically treated with two antimitotic agents, colchicine and oryzalin at different concentrations and times. After these treatments, nucellar embryos were successively cultured on MT medium containing 500 mg/liter^<-1> malt extract. Ploidy levels of seedlings were evaluated by both flow cytometry and chromosome counting of root tips. Frequency of tetraploid production depended on the kind of antimitotic agents as well as on the concentration and time of treatment. Among the treatment conditions tested, colchicine gave better results than oryzalin on inducing chromosome doubling. Tetraploid plants were obtained from approximately 50% of the nucellar seedlings treated with colchicine at 0.05% for 48 hours. These tetraploids showed poor growth during the early stage after acclimatization, but grew as vigorously as the diploid plants when grafted onto 3-year-old seedlings of trifoliate orange. These results showed that colchicine treatment combined with embryo culture is particularly efficient to induce chromosome doubling of nucellar embryos in Meiwa kumquat.
DOI： 10.2503/hrj.3.11
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Yahata Masaki, Oka Nobutaka, Kunitake Hisato, Yamaguchi Seiji, Komatsu Haruki
Horticultural research (Japan) 2 ( 4 ) 247 - 252 2003.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society for Horticultural Science
Relationship between weight of seeds and ploidy level of the seed-derived plantlets obtained from reciprocal crosses between diploid 'Banpeiyu' pummelo and two tetraploid varieties were examined to efficiently select triploid progenies for further breeding of the pummelo. Percentages of fully developed seeds obtained from the cross of 'Banpeiyu' pummelo with 4X Yuzu or 4X Natsudaidai were decreased by 48.0% and 8.4% as compared with the control, respectively. Seedlings obtained from these two crosses included triploids, tetraploids and hexaploid. 'Banpeiyu' pummelo as seed parent yielded a high percentage of triploids (88.0%) when crossed with 4X Yuzu, but low percentage (30.2%) when crossed with 4X Natsudaidai. In both crosses, the average weight of triploid seeds was significantly lighter than that of tetraploid seeds. Furthermore, all seedlings derived from undeveloped seeds were triploids. Percentages of fully developed seeds obtained from 4X Yuzu or 4X Natsudaidai X 'Banpeiyu' pummelo were not different from those of seeds from open pollinated fruits, but weights of these seeds were extremely decreased compared to seeds from open pollinated fruits. Approximately 50% of in vitro cultured seedlings obtained from both crosses were triploid, while the others were tetraploids, which might be derived from nucellar embryos of seed parents. There was no difference in the average weights of seeds between have seeds yielding triploid seedlings and those yielding tetraploids that might have originated from nucellar tissue.
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M. Yahata, N. Oka, H. Kunitake, H. Komatsu
2 ( 4 ) 247 - 252 2003.12
Language：Japanese Publishing type：Research paper (scientific journal)
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Yahata Masaki, Oka Nobutaka, Kunitake Hisato, Yamaguchi Seiji, Komatsu Haruki
Hort. Res. (Japan) 2 ( 4 ) 247 - 252 2003
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
Relationship between weight of seeds and ploidy level of the seed-derived plantlets obtained from reciprocal crosses between diploid 'Banpeiyu' pummelo and two tetraploid varieties were examined to efficiently select triploid progenies for further breeding of the pummelo. Percentages of fully developed seeds obtained from the cross of 'Banpeiyu' pummelo with 4X Yuzu or 4X Natsudaidai were decreased by 48.0% and 8.4% as compared with the control, respectively. Seedlings obtained from these two crosses included triploids, tetraploids and hexaploid. 'Banpeiyu' pummelo as seed parent yielded a high percentage of triploids (88.0%) when crossed with 4X Yuzu, but low percentage (30.2%) when crossed with 4X Natsudaidai. In both crosses, the average weight of triploid seeds was significantly lighter than that of tetraploid seeds. Furthermore, all seedlings derived from undeveloped seeds were triploids.<br> Percentages of fully developed seeds obtained from 4X Yuzu or 4X Natsudaidai × 'Banpeiyu' pummelo were not different from those of seeds from open pollinated fruits, but weights of these seeds were extremely decreased compared to seeds from open pollinated fruits. Approximately 50% of <i>in vitro</i> cultured seedlings obtained from both crosses were triploid, while the others were tetraploids, which might be derived from nucellar embryos of seed parents. There was no difference in the average weights of seeds between have seeds yielding triploid seedlings and those yielding tetraploids that might have originated from nucellar tissue.<br>
DOI： 10.2503/hrj.2.247
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共著者:Kunitake, H., K. Nagasawa, K. Takami, H. Komatsu
Plant Biotechnology 19 ( 5 ) 345 - 352 2002.12
Language：English Publishing type：Research paper (scientific journal)
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共著者:Rachanee C., H. Kunitake, N. Sattayasai, S. Nuchadomrong
Science Asia 28 ( 3 ) 221 - 225 2002.9
Language：English Publishing type：Research paper (scientific journal)
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Kunitake H., Nagasawa K., Komatsu H.
Plant Biotechnology 19 ( 5 ) 345 - 352 2002
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Plant Biotechnology
Somatic hybrids were produced between embryogenic callus protoplasts of 'Shogun' mandarin (Citrus reticulata Blanco, 2n=2x=18) and leaf protoplasts of grapefruit (C. paradisi Macf., 2n=2x=18) by electrofusion. Hybridity of the two regenerated plants was confirmed by leaf characteristics and nuclear genome analysis using RAPD and PCR RFLP. Flow cytometry and root-tip cell count of the two hybrids revealed a chromosome number of 27. These triploid somatic hybrids on transfer to field showed normal morphology and vigorous growth. Field performances as well as disease resistance are under investigation. © 2002, Japanese Society for Plant Cell and Molecular Biology. All rights reserved.
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Production of inter- specific hybrids between Lycoris incarnata and four other species through embryo culture. Reviewed
共著者:Biao Ma, I. Tarumoto, N. Nkakamura , H. Kunitake
Journal of the Japanese Society for Horticultural Science 70 ( 6 ) 697 - 703 2001.12
Language：English Publishing type：Research paper (scientific journal)
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Ma B., Tarumoto I., Nakamura N., Kunitake H.
Journal of the Japanese Society for Horticultural Science 70 ( 6 ) 697 - 703 2001.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of the Japanese Society for Horticultural Science
Interspecific crosses were made between a sterile species (L. incarnata) and four fertile species (L. sanguinea, L. sprengeri, L. radiata var. pumila (L. pumila, hereafter), and L. aurea) to breed varieties with new characters. Embryos, excised from ovules 20-30 days after pollination, were cultured on a modified MS medium containing 500 mg· liter -1 casein hydrolysate (Ma et al., 2001). Interspecific crosses using L. incarnata as a female parent yielded 26 hybrid plants, whereas that of L. sanguinea X L. incarnata produced none. Cytological studies revealed that all hybrids were triploid, which would have originated from the fertilization of an unreduced 2n - egg of the female parent (L. incarnata) and a normal haploid gamete of the male parent. The hybridity was also confirmed by isozyme analysis in 26 hybrid plants and partly by character observation in hybrid plants of L. incarnata X L. pumila.
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Effects of temperature and CO2 concentration on the growth of Citrus plantlets. "jointly worked" Reviewed
K. Nagasawa, H. Kunitake, H. Komatsu
Proceedings of the school of Agriculture, Kyushu Tokai University 20 ( 1 ) 9 - 14 2001.4
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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Production of triploid somatic hybrids between mandarin and grapefruit through electrofusion. Reviewed
H. Kunitake
Journal of Plant Biotechnology 2 ( 2 ) 109 - 113 2000.4
Language：English Publishing type：Research paper (scientific journal)
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Determination of ploidy levels of Citrus species using flow cytometry. "jointly worked" Reviewed
S. Harusaki, D. Kokuryo, H. Kunitake, H. Komatsu
Proceedings of the school of Agriculture, Kyushu Tokai University 19 ( 1 ) 45 - 52 2000.3
Language：Japanese Publishing type：Research paper (scientific journal)
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Survey and evaluation of old mango trees in Ishigaki city, Okinawa. "jointly worked" Reviewed
K. Sakihara, S. Ogawa, K. Kitajima, N. Nakazato, Y. Kawamitsu, H. Tsukayama, H. Kunitake, H. Komatsu
19 ( 1 ) 53 - 60 2000.3
Language：Japanese Publishing type：Research paper (scientific journal)
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Effect of in vitro environmental conditions on somatic embryogenesis and its germination from necellar calli of Ohta Ponkan(Citrus reticulata Blanco.)."jointly worked" Reviewed
Nagasawa, K., T. Kouichi, H. Kunitake, H. Komatsu
Proceedings of school of agriculture, Kyushu Tokai University 18 11 - 18 1999.3
Language：English Publishing type：Research paper (scientific journal)
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Polyphenols in Fararia x ananassa. "jointly worked" Reviewed
5 ( 2 ) 201 - 205 1998.12
Language：Japanese Publishing type：Research paper (scientific journal)