Papers - KUNITAKE Hisato
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Inoue K., Kato T., Nobukuni A., Kunitake H., Yabuya T.
Scientia Horticulturae 110 ( 4 ) 334 - 339 2006.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Scientia Horticulturae
Characteristics such as flower form, size and color of outer and inner perianths, anthocyanins in outer perianths, size, color and fertility of pollen and self-fertility of diploid and tetraploid lines regenerated via protoplast culture of Iris fulva were examined and compared with those of the diploid wild line. Among these characteristics, flower form, inner and outer perianth sizes of the tetraploid lines were noticeable, because these lines had upward flower forms and bigger flowers than diploid lines. Furthermore, reciprocal crosses between diploid or tetraploid lines of I. fulva and I. ensata and those of I. fulva and I. laevigata were performed. Three seedlings were obtained from the cross of tetraploid I. fulva × diploid I. laevigata through embryo rescue. One of them was identified as the interspecific hybrid between tetraploid I. fulva and I. laevigata by flow cytometoric (FCM), cytological and molecular (RAPD) analyses. This is the first report on production of hybrids from these lines. I. fulva has unique brown flowers, and this trait could be very useful for flower color breeding of I. laevigata which lacks this color. Therefore, the hybrid of I. fulva (4×) × I. laevigata may be the best available gene source for brown color breeding of this species. © 2006 Elsevier B.V. All rights reserved.
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Yahata, M., H. Kunitake, K. Yasuda, K. Yamashita, H. Komatsu, R. Matsumoto
Journal of American Society for Horticultural Science 131 ( 6 ) 764 - 769 2006.11
Language：English Publishing type：Research paper (scientific journal)
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Characterization of tetraploid plants regenerated via protoplast culture of Iris falva and their crossability with Japanese irises. （共著） Reviewed
K. Inoue, T. Kato, N. sami, H. Kunitake, T. Yabuya
Scientia Horticulturae 110 334 - 339 2006.11
Language：English Publishing type：Research paper (scientific journal)
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Yahata M., Kunitake H., Yasuda K., Yamashita K., Komatsu H., Matsumoto R.
Journal of the American Society for Horticultural Science 131 ( 6 ) 764 - 769 2006.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of the American Society for Horticultural Science
The reciprocal crosses between two citrus cultivars and Citropsis schweinfurthii (Engl.) Swing. & M. Kell. were conducted. The cross between 'Nanpu' tangor {'Kiyomi' tangor (Citrus unshiu Marc. × C. sinensis Osbeck) × 'Fairchild' tangerine-tangelo [clementina (C. clementina hort. ex Tanaka) × 'Orlando' tangelo (C. paradisi Macf. × C. reticulata Blanco)]} and C. schweinfurthii produced some developed seeds with an average weight approximately 1/10 of that of the seeds obtained from open pollination in 'Nanpu' tangor. These seeds germinated on Murashige and Tucker medium, and three and 28 seedlings were obtained from crosses using C. schweinfurthii as the female and the male parent, respectively. The absolute nuclear genome size of these seedlings [∼0.84 pg of DNA content per somatic nucleus (2C)] was intermediate of that of the 'Nanpu' tangor (0.78 pg/2C) and C. schweinfurthii (0.90 pg/2C) seedlings. The chromosome counts of the young leaves revealed that they were diploids (2n = 2X = 18). Furthermore, the hybridity of the seedlings obtained from the reciprocal crosses between 'Nanpu' tangor and C. schweinfurthii was confirmed by randomly amplified polymorphic DNA (RAPD) analysis and cleaved amplified polymorphic sequence (CAPS) analysis. These hybrids will be utilized as important materials for investigating the phylogenic relationships between these genera in the subfamily Aurantioideae.
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Kunitake, H., H. Tsuda, R. Takaki, Y. Ohno, Y. Kuroki, K. Yoshioka, T. Kage, T. Ito, H. Komatsu
Horticultural Research 5 ( 2 ) 105 - 110 2006.6
Language：Japanese Publishing type：Research paper (scientific journal)
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Kunitake Hisato, Tsuda Hirotoshi, Takagi Ryoko, Ohno Yukinori, Kuroki Yoshikazu, Yoshioka Katsunori, Kage Tetsurou, Ito Toshiaki, Komatsu Haruki
Hort. Res. (Japan) 5 ( 2 ) 105 - 110 2006
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
The possibility of using wild blueberry Shashanbo (Vaccinium bracteatum Thunb.) as a rootstock for cultivating northern highbush blueberry in a warm region was investigated. The survival ratios of three different cultivars ('Berkeley', 'Bluecrop', and 'Earliblue') grafted on 3-year-old seedlings of Shashanbo plants were 85.7 to 100%. Furthermore, 41 cultivars included in highbush blueberries and rabbiteye blueberries were successfully grafted on seedlings of Shashanbo rootstock. There were no signs of incompatibility even four years after grafting, and anatomical observations of graft unions supported this result. The shoot growth and fruit weight of 'Earliblue' grafted on seedlings of Shashanbo rootstock were significantly higher than those of plants on their own roots. However, the shoot growth of 'Earliblue' grafted on rooted cuttings of rabbiteye blueberries ('Homebell' and 'Tifblue') was significantly higher than that of those on seedlings of Shashanbo rootstock. In addition, there were no differences in concentrations or component ratio of sugars and organic acids on any rootstock. These findings suggest that Shashanbo has graft compatibility with blueberry and that seedlings could be used as a rootstock for cultivating northern highbush blueberries in southern Kyushu.
DOI： 10.2503/hrj.5.105
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共著者：Yahata, M., H. Kunitake, T. Yabuya, K. Yamashita, Y. Kashihara, H. Komatsu.
Journal of the American Society for Horticultural Science 130 ( 1 ) 34 - 40 2005.11
Language：English Publishing type：Research paper (scientific journal)
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Yahata M., Kunitake H., Yabuya T., Yamashita K., Kashihara Y., Komatsu H.
Journal of the American Society for Horticultural Science 130 ( 6 ) 899 - 903 2005.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of the American Society for Horticultural Science
To produce the homozygous strain of a haploid plant derived from small seed-derived seedlings of 'Banpeiyu' pummelo (Citrus grandis Osbeck), we carried out colchicine treatment to axillary shoot buds of the haploid. Many shoots with cytochimeras (X+2X and 2X+4X) arose from the colchicine-treated axillary buds. When cytochimeric buds of 2X+4X were top-grafted onto trifoliate orange [Poncirus trifoliata (L.) Raf.], a complete diploid shoot with 18 chromosomes was obtained from the cytochimera. This diploid strain showed vigorous growth compared with the original haploid. The leaf weight per unit area and the stomata size in this diploid were significantly larger than those of the original haploid plant, and were almost equal to those of 'Banpeiyu' pummelo. The diploid strain was confirmed to be a doubled haploid of a haploid from 'Banpeiyu' pummelo, based on random amplified polymorphic DNA (RAPD) analysis and chromosome composition analysis by chromomycin A 3 (CMA) staining.
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共著者：Yahata, M., H. Kurogi, H. Kunitake, K. Nagano, T. Yabuya, K. Yamashita , H. Komatsu
Journal of the Japanese Society for Horticultural Science 74 ( 4 ) 281 - 288 2005.7
Language：English Publishing type：Research paper (scientific journal)
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Yahata M., Kurogi H., Kunitake H., Nagano K., Yabuya T., Yamashita K., Komatsu H.
Journal of the Japanese Society for Horticultural Science 74 ( 4 ) 281 - 288 2005.7
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of the Japanese Society for Horticultural Science
To evaluate the reproductive potential of female and male gametes in a haploid plant derived from seedlings of 'Banpeiyu' pummelo, we performed crosses between the haploid and several diploid citrus cultivars. In the crosses with the diploid cultivars as pollen parents, no fruit set on the haploid in all cross combinations. However, developed seeds were obtained in four cross combinations, when some monoembryonic diploid cultivars were pollinated with pollen of the haploid. These seeds germinated normally and developed into diploid, vigorous seedlings with large wing leaves, typical of 'Banpeiyu' pummelo. Furthermore, the hybridity of a seedling obtained from the cross between 'Kiyomi' tangor and the haploid was confirmed by random amplified polymorphic DNA (RAPD) analysis and chromosome composition by chromomycin A 3 (CMA) staining. These results suggest that the haploid produced fertile pollen grains (n=9).
DOI： 10.2503/jjshs.74.281
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K. Kawase, M. Yahata, S. Nakagawa, K. Haraguchi, H. Kunitake
Horticultural Research 4 ( 2 ) 141 - 146 2005.6
Language：Japanese Publishing type：Research paper (scientific journal)
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共著者：Takami, K., A. Matsumaru, M.Yahata, H. Kunitake, H. Komatsu.
Sexual Plant Reproduction 18 ( 1 ) 21 - 28 2005.6
Language：English Publishing type：Research paper (scientific journal)
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Takami K., Matsumaru A., Yahata M., Kunitake H., Komatsu H.
Sexual Plant Reproduction 18 ( 1 ) 21 - 28 2005.6
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Sexual Plant Reproduction
Embryogenic protoplasts of 'Shogun' mandarin (Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts from Citropsis gabunensis Swing. & M. Kell, and two green embryoids were regenerated after 3 months of culture. Two months after transfer to the regeneration medium, numerous plantlets were obtained from the embryoids. These plants grew vigorously, had well-developed root systems, and exhibited leaf characteristics intermediate to those of the parents. The absolute nuclear genome size of the regenerated plant SH2 (1.75 pg/4C) was the sum of those of the 'Shogun' mandarin (0.75 pg/2C) and C. gabunensis (0.97 pg/2C). The chromosome counts of the young leaves revealed that they were tetraploids (2n=4x=36). Random amplified polymorphic DNA (RAPD) analysis of the two lines (SH1 and SH2) verified their hybridity. Cytoplasmic genome analysis using universal primers reveal that their chloroplast (cp) DNA banding pattern is identical to that of C. gabunensis, while the banding pattern of the mitochondrial (mt) DNA is identical to that of the 'Shogun' mandarin. These somatic hybrids are important materials for investigating the phylogenetic relationships between these two genera in the subfamily Aurantioideae. © Springer-Verlag 2005.
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共著者：Yahata, M., S. Harusaki, K. Takami, P. Toolapong, H. Kunitake, T. Yabuya, K. Yamashita , H. Komatsu
Journal of the American Society for Horticultural Science 130 ( 1 ) 34 - 40 2005.1
Language：English Publishing type：Research paper (scientific journal)
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Yahata M., Harusaki S., Komatsu H., Takami K., Kunitake H., Yabuya T., Yamashita K., Toolapong P.
Journal of the American Society for Horticultural Science 130 ( 1 ) 34 - 40 2005.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Journal of the American Society for Horticultural Science
The morphological characteristics and reproductive potential of a haploid plant obtained from the cross between 'Banpeiyu' pummelo (Citrus grandis) and 'Ruby Red' grapefruit (C. paradisi Macf.) were investigated. The haploid was confirmed to be derived from female gamete of 'Banpeiyu' pummelo by isozyme and random amplified polymorphic DNA (RAPD) analysis. Flow cytometry analysis revealed that the haploidy was maintained in several tissues and organs of this plant. It also had the typical morphology of a haploid, such as small leaves and flowers, and had slightly fertile pollen grains. Furthermore, diploid progenies were obtained from the cross between 'Kiyomi' tangor and the haploid. The hybridity of these seedlings was confirmed by assessment of the leaf characteristics and RAPD analysis. These results suggest that this haploid produced fertile unreduced pollen grains (n = 9).
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Kunitake Ayumi, Iwasaki Tatsuma, Hidaka Nami, Nagamachi Shigeki, Katsuki Hiroshi, Uno Takeshi, Takasaki Mayumi
PAIN RESEARCH 20 ( 3 ) 117 - 125 2005
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：日本疼痛学会
The effects of spinal cord stimulation (SCS) on the neuronal activity of the brain were examined by single photon emission computed tomography (SPECT) in patients with chronic neuropathic pain. Regional cerebral blood flow (CBF) in each cortical area and the thalamus decreased in several patients without SCS. Patients with central pain due to thalamic hemorrhage showed a decrease in rCBF in the thalamus contralateral to the painful side. During the stimulation period in SCS, parietal rCBF decreased on the side contralateral to the pain. In contrast, rCBF increased in the bilateral frontal and anterior cingulate cortex and in the contralateral temporal lobe in half of the patients in whom SCS was effective in relieving pain. The decrease in thalamic rCBF in two patients with central pain was improved by the SCS therapy; however, pain was relieved in only one of them. In the majority of patients in whom SCS was not effective, there was no change in rCBF in various cortical areas, even after SCS. These results suggest that, in patients with chronic neuropathic pain, SCS modulates the neuronal activities of several brain areas that are believed to be associated with pain processing.
DOI： 10.11154/pain.20.117
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河瀬 憲次, 八幡 昌紀, 中川 匠子, 原口 加奈, 國武 久登
園芸学研究 4 ( 2 ) 141 - 146 2005
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
実生の根の形態を指標としてニンポウキンカンの珠心胚実生から四倍体を選抜し, その形態調査を行った.<br>ニンポウキンカンの完全種子を播種して得られた457本の実生から, 太くて短い細根を有する1個体を見出した. この実生の倍数性を調査した結果, 36本の染色体を有しており, 四倍体であることが明らかとなった. また, RAPD分析により, この四倍体はニンポウキンカンの珠心胚由来であることが推察された. この四倍体の形態について調査したところ, 二倍体と比べ, 葉は丸くて厚く, 気孔, 花および花粉は大きくなっており, 四倍体特有の形態を示した. 四倍体の果実は, 果実の重さや大きさにおいて二倍体と同等の形質を示した. さらに, 可食部である果皮が厚くなり, 完全種子数が少なくなっていた. 将来, 本研究で得られたニンポウキンカンの四倍体は直接的な品種としての利用や三倍体育種の親として重要な素材となるであろう.
DOI： 10.2503/hrj.4.141
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二倍体カンキツ品種との交雑による半数体ブンタンの生殖機能の評価
八幡 昌紀, 黒木 宏憲, 國武 久登, 長野 克也, 藪谷 勤, 山下 研介, 小松 春喜
園藝學會雜誌 74 ( 4 ) 281 - 288 2005
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：園藝學會
'晩白柚'の実生から得られた半数体における雌性および雄性配偶子の生殖機能を評価するために, 半数体と種々の二倍体カンキツ品種との交雑を行った.半数体に二倍体の花粉を授粉した場合では全く着果しなかったが, 半数体を花粉親とした場合, 4つの交雑組合せにおいて完全種子が得られた.これらの完全種子は正常に発芽し, 多くの二倍体実生が得られた.これらの実生は旺盛に成長し, '晩白柚'の形態的特徴である翼葉を有していた.さらに, '清見'と半数体との交雑から得られた1個体の実生について, RAPD分析およびCMA染色による染色体構成を解析したところ, 雑種であることが確認された.これらの結果から, 本半数体では正常な花粉(n=9)が形成されていることが示唆された.
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Kawase Kenji, Yahata Masaki, Nakagawa Syoko, Haraguchi Kana, Kunitake Hisato
Horticultural research (Japan) 4 ( 2 ) 141 - 146 2005
Language：Japanese Publishing type：Research paper (scientific journal)
A tetraploid of Meiwa kumquat (Fortunella crassifolia Swingle) was selected from nucellar seedlings using root morphology, and its morphological characteristics were investigated. One seedling with thick short rootlets was obtained from 457 seedlings. Flow cytometry and chromosome observation demonstrated that the seedling was tetraploid (2n=4X=36). This tetraploid was presumed by Randam Amplified Polymorphic DNA (RAPD) analysis to have derived from nucellar embryos. The tetraploid showed similar morphology to other Citrus tetraploids such as thick, round leaves and larger stomata, flowers, and pollen grains compared with the original diploid. The tetraploid fruit was almost equal in weight and size to that of the diploid, but had a thicker peel and smaller number of developed seeds than the diploid fruit. In the future, the tetraploid obtained in the present study will be important material as a new cultivar and a parent of triploid breeding.
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Efficient Production of Polyploid Plants via Protoplast Culture of Iris fulva
INOUE Kouichi, KATO Tomomi, KUNITAKE Hisato, YABUYA Tsutomu
Cytologia : international journal of cytology 69 ( 3 ) 327 - 333 2004.11
Language：Japanese Publishing type：Research paper (scientific journal)