論文 - 岡林 環樹
-
Inagaki H., Saito A., Kaneko C., Sugiyama H., Okabayashi T., Fujimoto S.
Pathogens 10 ( 6 ) 2021年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Pathogens
More than 1 year has passed since social activities have been restricted due to the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). More recently, novel SARS-CoV-2 variants have been spreading around the world, and there is growing concern that they may have higher transmissibility and that the protective efficacy of vaccines may be weaker against them. Immediate measures are needed to reduce human exposure to the virus. In this study, the antiviral efficacy of deep-ultraviolet light-emitting diode (DUV-LED) irradiation (280 ± 5 nm, 3.75 mW/cm2 ) against three SARS-CoV-2 variants was evaluated. For the B.1.1.7, B.1.351, and P.1 variant strains, irradiation of the virus stocks for 1 s resulted in infectious titer reduction rates of 96.3%, 94.6%, and 91.9%, respectively, and with irradiation for 5 s, the rates increased to 99.9%, 99.9%, and 99.8%, respectively. We also tested the effect of pulsed DUV-LED irradiation (7.5 mW/cm2, duty rate: 50%, frequency: 1 kHz) under the same output conditions as for continuous irradiation and found that the antiviral efficacy of pulsed and continuous irradiation was the same. These findings suggest that by further developing and optimizing the DUV-LED device to increase its output, it may be possible to instantly inactivate SARS-CoV-2 with DUV-LED irradiation.
-
Fahkrajang W., Sudaryatma P.E., Mekata H., Hamabe S., Saito A., Okabayashi T.
Veterinary Microbiology 255 109017 2021年4月
担当区分：責任著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Microbiology
Bovine coronavirus (BCoV) is one of the agents causing bovine respiratory disease complex (BRDC), with single infection tending to be mild to moderate; the probability of developing pneumonia in BRDC may be affected by viral and bacterial combinations. Previously, we reported that bovine respiratory syncytial virus (BRSV) infection enhances adherence of Pasteurella multocida (PM) to cells derived from the bovine lower respiratory tract but that BRSV infection in cells derived from the upper respiratory tract reduces PM adherence. In this study, we sought to clarify whether the modulation of bacterial adherence to cells derived from the bovine upper and lower respiratory tract is shared by other BRDC-related viruses by infecting bovine epithelial cells from the trachea, bronchus and lung with BCoV and/or PM. The results showed that cells derived from both the upper and lower respiratory tract were susceptible to BCoV infection. Furthermore, all cells infected with BCoV exhibited increased PM adherence via upregulation of two major bacterial adhesion molecules, intercellular adhesion molecule-1 (ICAM-1) and platelet-activating factor receptor (PAF-R), suggesting that compared with BRSV infection, BCoV infection differentially modulates bacterial adherence. In summary, we identified distinct interaction between bovine respiratory viruses and bacterial infections.
-
Nagai M, Okabayashi T, Akagami M, Matsuu A, Fujimoto Y, Hashem MA, Mekata H, Nakao R, Matsuno K, Katayama Y, Oba M, Omatsu T, Asai T, Nakagawa K, Ito H, Madarame H, Kawai K, Ito T, Nonaka N, Tsukiyama-Kohara K, Inoshima Y, Mizutani T, Misawa N
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 88 104664 2021年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Infection, Genetics and Evolution
Recently, hepe-astrovirus-like RNA viruses named bastroviruses (BastVs), have been found in human, pig, bat, and rat fecal samples. In this study, we determined nearly complete genome sequences of four BastVs in the feces of healthy pigs. Genetic characterization revealed that these porcine BastVs (PBastVs) and BastVs from other animals including humans, had the same genome organization, that is, they contained three predicted conserved domains of viral methyltransferase, RNA helicase, and RdRp in the nonstructural ORF1 and the astrovirus capsid domain in the structural ORF2. Phylogenetic analyses using RNA-dependent RNA polymerase and the capsid region revealed that PBastVs branched with bat and rat BastVs; however, the groups formed by each host were distantly related to human BastVs. Pairwise amino acid sequence comparison demonstrated that PBastVs shared 95.2–98.6% and 76.1–95.5% sequence identity among each other in the ORF1 and ORF2 regions, respectively; the sequence identities between PBastVs and BastVs from other animals were 21.4–42.5% and 9.1–20.6% in the ORF1 and ORF2 regions, respectively. This suggested that BastVs were derived from a common ancestor but evolved independently in each host population during a prolonged period. Putative recombination events were identified in the PBastV genome, suggesting that PBastVs gain sequence diversity and flexibility through recombination events. In an analysis of previously obtained metagenomic data, PBastV sequence reads were detected in 7.3% (23/315) of fecal samples from pigs indicating that PBastVs are distributed among pig populations in Japan.
-
Kabali E., Pandey G.S., Munyeme M., Kapila P., Mukubesa A.N., Ndebe J., Muma J.B., Mubita C., Muleya W., Muonga E.M., Mitoma S., Hang’ombe B.M., Wiratsudakul A., Ngan M.T., Elhanafy E., El Daous H., Huyen N.T., Yamazaki W., Okabayashi T., Abe M., Norimine J., Sekiguchi S.
Antibiotics 10 ( 3 ) 1 - 17 2021年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Antibiotics
A cross-sectional study was used to identify and assess prevalence and phenotypic antimicrobial resistance (AMR) profiles of Escherichia coli and other enterobacteria isolated from healthy wildlife and livestock cohabiting at a 10,000 acres game ranch near Lusaka, Zambia. Purposive sampling was used to select wildlife and livestock based on similarities in behavior, grazing habits and close interactions with humans. Isolates (n = 66) from fecal samples collected between April and August 2018 (n = 84) were examined following modified protocols for bacteria isolation, biochemical identification, molecular detection, phylogenetic analysis, and antimicrobial susceptibility testing by disc diffusion method. Data were analyzed using R software, Genetyx ver.12 and Mega 6. Using Applied Profile Index 20E kit for biochemical identification, polymerase chain reaction assay and sequencing, sixty-six isolates were identified to species level, of which Escherichia coli (72.7%, 48/66), E. fergusonii (1.5%, 1/66), Shigella sonnei (22.7%, 14/66), Sh. flexinerri (1.5%, 1/66) and Enterobacteriaceae bacterium (1.5%, 1/66), and their relationships were illustrated in a phylogenetic tree. Phenotypic antimicrobial resistance or intermediate sensitivity expression to at least one antimicrobial agent was detected in 89.6% of the E. coli, and 73.3% of the Shigella isolates. The E. coli isolates exhibited the highest resistance rates to ampicillin (27%), ceftazidime (14.3%), cefotaxime (9.5%), and kanamycin (9.5%). Multidrug resistance (MDR) was detected in 18.8% of E. coli isolates while only 13.3% Shigella isolates showed MDR. The MDR was detected among isolates from impala and ostrich (wild animals in which no antimicrobial treatment was used), and in isolates from cattle, pigs, and goats (domesticated animals). This study indicates the possible transmission of drug-resistant microorganisms between animals cohabiting at the wildlife-livestock interface. It emphasizes the need for further investigation of the role of wildlife in the development and transmission of AMR, which is an issue of global concern.
-
Sato Y, Mekata H, Sudaryatma PE, Kirino Y, Yamamoto S, Ando S, Sugimoto T, Okabayashi T
Vector borne and zoonotic diseases (Larchmont, N.Y.) 21 ( 5 ) 378 - 384 2021年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Vector-Borne and Zoonotic Diseases
Severe fever with thrombocytopenia syndrome (SFTS), caused by Dabie bandavirus, generally called SFTS virus (SFTSV), is an emerging zoonosis in East Asia. In Japan, 50-100 cases of SFTS have been reported each year since the first case was reported in 2013. SFTS is a tick-borne infectious disease, and SFTSV has been isolated from ticks in China and South Korea. Haemaphysalis longicornis and Amblyomma testudinarium are considered the primary vectors in Japan. However, the other tick species seldom feeding on humans might also play an important role in maintaining the virus in nature. In this study, we collected ticks on vegetation around the location where two SFTS patients were estimated to have been infected in Miyazaki Prefecture, Japan, isolated live SFTSV, and performed a phylogenetic analysis. A total of 257 ticks were collected, and SFTSV RNA was detected in 19.5% (9/46) of tick pools. A total of 10 infectious SFTSVs were successfully isolated from A. testudinarium, Haemaphysalis flava, Haemaphysalis formosensis, Haemaphysalis hystricis, and Haemaphysalis megaspinosa. Furthermore, the whole viral sequences isolated from ticks were highly homologous to sequences isolated from SFTS patients in the same sampling area in the past. These results suggest that SFTSVs are maintained in these tick species in the sampling area and sporadically transmitted to humans. Surveillance of SFTSV in ticks provides important information about the risk of incidental transmission to humans.
-
Kirino Y, Ishijima K, Miura M, Nomachi T, Mazimpaka E, Sudaryatma PE, Yamanaka A, Maeda K, Sugimoto T, Saito A, Mekata H, Okabayashi T
Viruses 13 ( 2 ) 2021年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Viruses
Severe fever with thrombocytopenia syndrome virus (SFTSV) is the causative agent of SFTS, an emerging tick-borne disease in East Asia, and is maintained in enzootic cycles involving ticks and a range of wild animal hosts. Direct transmission of SFTSV from cats and dogs to humans has been identified in Japan, suggesting that veterinarians and veterinary nurses involved in smallanimal practice are at occupational risk of SFTSV infection. To characterize this risk, we performed a sero-epidemiological survey in small-animal-practice workers and healthy blood donors in Miyazaki prefecture, which is the prefecture with the highest per capita number of recorded cases of SFTS in Japan. Three small-animal-practice workers were identified as seropositive by ELISA, but one had a negative neutralization-test result and so was finally determined to be seronegative, giving a seropositive rate of 2.2% (2 of 90), which was significantly higher than that in healthy blood donors (0%, 0 of 1000; p < 0.05). The seroprevalence identified here in small-animal-practice workers was slightly higher than that previously reported in other high-risk workers engaged in agriculture and forestry in Japan. Thus, enhancement of small-animal-practice workers’ awareness of biosafety at animal hospitals is necessary for control of SFTSV.
DOI： 10.3390/v13020229
-
Ito S, Hirai T, Hamabe S, Subangkit M, Okabayashi T, Goto Y, Nishida S, Kurita T, Yamaguchi R
The Journal of veterinary medical science 83 ( 1 ) 146 - 150 2021年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本獣医学会
On a coastline in Miyazaki Prefecture, Japan, a wild subadult female striped dolphin was found dead. Necropsy revealed poor nutritional status and bilateral pneumonia, which was histologically diagnosed as severe suppurative necrotizing bronchopneumonia. Special staining detected numerous intralesional filamentous, branching bacteria, which was identified as <i>Nocardia cyriacigeorgica</i> by sequencing of 16S ribosomal RNA and gyrB genes. Other main histological findings included lymphoid depletion in the spleen and superficial cervical and pulmonary lymph nodes. Suppurative nocardiosis without a granulomatous reaction is uncommon, and it is assumed its pathogenesis was related to the host's immune status. This paper discusses the variable inflammatory response to nocardiosis and describes the first case of <i>N. cyriacigeorgica</i> infection in a wild striped dolphin in Japan.
DOI： 10.1292/jvms.20-0234
-
Rapid Inactivation of SARS-CoV-2 with Ozonated Water
Inagaki H., Saito A., Sudaryatma P.E., Sugiyama H., Okabayashi T., Fujimoto S.
Ozone: Science and Engineering 43 ( 3 ) 208 - 212 2021年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Ozone: Science and Engineering
Although ozonated water is one of the promising candidates for hand hygiene to prevent fomite infection, the detailed effects of ozonated water on SARS-CoV-2 have not been clarified. We evaluated the inactivating effect of ozonated water against SARS-CoV-2 by its concentration and exposure time. The reduction rates of virus titer after 5 sec treatment with ozone concentrations of 1, 4, 7, and 10 mg/L were 81.4%, 93.2%, 96.6%, and 96.6%, respectively. No further decrease in virus titer was observed by the extended exposure time over 5 sec. High-concentration ozonated water, such as more than 7 mg/L, was considered to be effective in promptly inactivating SARS-CoV-2 virus in aqueous solution.
-
Kirino Y., Yamamoto S., Nomachi T., Mai T.N., Sato Y., Sudaryatma P.E., Norimine J., Fujii Y., Ando S., Okabayashi T.
Veterinary Medicine and Science 8 ( 2 ) 877 - 885 2021年
担当区分：責任著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Medicine and Science
Background: Miyazaki Prefecture is one of the hotspots of severe fever with thrombocytopenia syndrome (SFTS) cases and related deaths in Japan since 2013 and other pathogens of tick-borne diseases (TBDs). Japanese spotted fever and scrub typhus are also endemic in this region. Objectives: A total of 105 wild boars, hunted in 2009, were serologically examined as sentinels for TBDs to indirectly demonstrate the potential hazard of ticks transmitting pathogens to humans in the studied area. Methods: The collected blood and spleens of the wild boars underwent serological and molecular tests for SFTSV, Rickettsia japonica (Rj) [antibody to spotted fever group rickettsiae (SFGR) were tested by using species-common antigen], and Orientia tsutsugamushi (Ot). Results: Seroprevalences of SFTSV, SFGR, and Ot were 41.9%, 29.5%, and 33.3%, respectively. SFTS viral RNA was identified in 7.6% of the sera, whereas DNA of Rj or Ot was not detected in any sample. In total, 43.8% of the boars possessed an infection history with SFTSV (viral gene and/or antibody). Of these, 23.8% had multiple-infection history with SFGR and/or Ot. Conclusions: The high prevalence of SFTSV in wild boars might reflect the high risk of exposure to the virus in the studied areas. In addition, SFTSV infection was significantly correlated with Ot infection, and so were SFGR infection and Ot infection, indicating that these pathogens have common factors for infection or transmission. These data caution of the higher risk of SFTSV infection in areas with reported cases of other TBDs.
DOI： 10.1002/vms3.696
-
岡林 環樹
産学官連携ジャーナル 17 ( 9 ) 25 - 28 2021年
担当区分：筆頭著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：国立研究開発法人 科学技術振興機構
※本記事に抄録はありません。
-
Rapid inactivation of SARS-CoV-2 with deep-UV LED irradiation. 査読あり
Saito A2,
Emerging Microbes and Infections 2020年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
-
Sekiguchi Y., Nagata A., Sunaga F., Oi T., Imai R., Madarame H., Katayama Y., Oba M., Okabayashi T., Misawa N., Oka T., Mizutani T., Nagai M.
Archives of Virology 165 ( 12 ) 2909 - 2914 2020年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Archives of Virology
© 2020, Springer-Verlag GmbH Austria, part of Springer Nature. Two and three genotypes of enterovirus G (EV-G) carrying a papain-like cysteine protease (PL-CP) sequence were detected on two pig farms and classified into genotypes G1 and G10, and G1, G8, and G17, respectively, based on VP1 sequences. A G10 EV-G virus bearing a PL-CP sequence was detected for the first time. Phylogenetic analysis of the P2 and P3 regions grouped the viruses by farm with high sequence similarity. Furthermore, clear recombination break points were detected in the 2A region, suggesting that PL-CP EV-G-containing strains gained sequence diversity through recombination events among the multiple circulating EV-G genotypes on the farms.
-
Yamanaka A., Kirino Y., Fujimoto S., Ueda N., Himeji D., Miura M., Sudaryatma P.E., Sato Y., Tanaka H., Mekata H., Okabayashi T.
Emerging Infectious Diseases 26 ( 12 ) 2994 - 2998 2020年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Emerging Infectious Diseases
© 2020 Centers for Disease Control and Prevention (CDC). All rights reserved. Two veterinary personnel in Japan were infected with severe fever with thrombocytopenia syndrome virus (SFTSV) while handling a sick cat. Whole-genome sequences of SFTSV isolated from the personnel and the cat were 100% identical. These results identified a nosocomial outbreak of SFTSV infection in an animal hospital without a tick as a vector.
-
Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012 査読あり
Atsushi Yamanaka, Mami Matsuda , Tamaki Okabayashi, Pannamthip Pitaksajjakul , Pongrama Ramasoota , Kyoko Saito, Masayoshi Fukasawa, Kentaro Hanada, Tomokazu Matsuura, Masamichi Muramatsu, Tatsuo Shioda, Ryosuke Suzuki
mSphere 6 ( 4 ) 2020年8月
-
Heavy Metals in Tuna Species Meat and Potential Consumer Health Risk: A Review
Norhazirah A.A., Shazili N.A.M., Kamaruzzaman Y., Sim S.F., Ahmad A., Ong M.C.
IOP Conference Series: Earth and Environmental Science 494 ( 1 ) 2020年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：IOP Conference Series: Earth and Environmental Science
Marine fishes are one of the protein sources and they form a significant part of human diet around the world. In particular, tuna fisheries are considered as the largest and most specialized commercially important group of species among all commercial fishes. The preparing tuna methods such as cooking and canning might alter the level of heavy metals inside the meat. The heat that applied to the meat might be decreased the moisture content in tuna meat, thus give some effects to the heavy metal level. Other than that, the different composition and function of different parts of tuna can contribute to the various level of heavy metals analysed. The metal contamination in tuna species meat has put serious question to the safety level of fish intake to the community. It represent an abiding threat to human health as it has been linked to some adverse health effects such as mental retardation, kidney damage, and various types of cancer and even worse, death could occur.The following review articles presents the findings of the work carried out by the various researchers in the past on the heavy metal pollution in samples of Tuna species around the world.
-
Bovine respiratory syncytial virus enhances the adherence of Pasteurella multocida to bovine respiratory tract epithelial cells by upregulating the Platelet-acting factor receptor. 査読あり
Sudaryatma P., Saito A., Mekara H., Kubo M., Fahkrajang W., Mazimpaka E., Okabayashi T.
Frontiers in Microbiology 2020年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
-
Molecular epidemiological survey and phylogenetic analysis of bovine respiratory coronavirus in Japan from 2016 to 2018 査読あり
Hirohisa MEKATA, Saori HAMABE, Putu Eka SUDARYATMA, Ikuo KOBAYASHI, Toru KANNO, Tamaki OKABAYASHI
2020年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1292/jvms.19-0587
-
Bovine respiratpry Syncytiak Virus decreased Pasteurella multocida adherence by downregulation the expression of intercellurar adhesion molecule-1 on the surface of upper respiratory epitheliacl cells. 査読あり
Sudaryatma PE,Saito A,Mekata H,Kubo M,Fahkrajang W,Tamaki Okabayashi T
Veterinary Microbiology 2020年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
-
Nagata A., Sekiguchi Y., Oi T., Sunaga F., Madarame H., Imai R., Sano K., Katayama Y., Omatsu T., Oba M., Furuya T., Shirai J., Okabayashi T., Misawa N., Oka T., Mizutani T., Nagai M.
Journal of General Virology 101 ( 8 ) 840 - 852 2020年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of General Virology
© 2020 Microbiology Society. All rights reserved. The genetic diversity of enterovirus G (EV-G) was investigated in the wild-boar population in Japan. EV-G-specific reverse transcription PCR demonstrated 30 (37.5%) positives out of 80 faecal samples. Of these, viral protein 1 (VP1) fragments of 20 samples were classified into G1 (3 samples), G4 (1 sample), G6 (2 samples), G8 (4 samples), G11 (1 sample), G12 (7 samples), G14 (1 sample) and G17 (1 sample), among which 11 samples had a papain-like cysteine protease (PL-CP) sequence, believed to be the first discoveries in G1 (2 samples) or G17 (1 sample) wild-boar EV-Gs, and in G8 (2 samples) or G12 (6 samples) EV-Gs from any animals. Sequences of the non-structural protein regions were similar among EV-Gs possessing the PL-CP sequence (PL-CP EV-Gs) regardless of genotype or origin, suggesting the existence of a common ancestor for these strains. Interestingly, for the two G8 and two G12 samples, the genome sequences contained two versions, with or without the PL-CP sequence, together with the homologous 2C/PL-CP and PL-CP/3A junction sequences, which may explain how the recombination and deletion of the PL-CP sequences occured in the PL-CP EV-G genomes. These findings shed light on the genetic plasticity and evolution of EV-G.
DOI： 10.1099/jgv.0.001446
-
Slaughterhouse survey for detection of bovine viral diarrhea infection among beef cattle in Kyushu, Japan. 査読あり
Agah MA, Notsu K, El-Khaiat HM, Arikawa G, Kubo M, Mitoma S, Okabayashi T, Mekata H, Elhanafy E, El Daous H, Mai TN, Nguyen TH, Isoda N, Sakoda Y, Norimine J, Sekiguchi S
Journal of Veterinary Medical Science 2019年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）