論文 - 岡林 環樹
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Sudaryatma P., Mekata H., Kubo M., Subangkit M., Goto Y., Okabayashi T.
Veterinary Microbiology 235 80 - 85 2019年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Microbiology
© 2019 Bovine respiratory disease complex is a major disease affecting the global cattle industry. Multiple infections by viruses and bacteria increase disease severity. Previously, we reported that bovine respiratory syncytial virus (BRSV) infection increases adherence of Pasteurella multocida to human respiratory and bovine kidney epithelial cells. To examine the interaction between the virus and bacteria in bovine respiratory cells, we generated respiratory epithelial cell lines from bovine trachea (bTEC), bronchus (bBEC), and lung (bLEC). Although all established cell lines were infected by BRSV and P. multocida susceptibility differed according to site of origin. The cells derived from the lower respiratory tract (bBEC and bLEC) were significantly more susceptible to BRSV than those derived from the upper respiratory tract (bTEC). Pre-infection of bBEC and bLEC with BRSV increased adherence of P. multocida; this was not the case for bTEC. These results indicate that BRSV may reproduce better in the lower respiratory tract and encourage adherence of bacteria. Thus, we identify one possible mechanism underlying severe pneumonia.
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Development of a LAMP assay for rapid and sensitive detection and differentiation of Mycobacterium avium subsp. avium and subsp. hominissuis. 査読あり
Yashiki N, Yamazaki Y, Subangkit M, Okabayashi T, Yamazaki W, Goto Y
Letters Appl. Microbiol. 2019年6月
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Genotyping of swine Mycobacterium avium subsp. hominissuis isolates from Kyushu, Japan. 査読あり
Subangkit M, Yamamoto T, Ishida M, Nomura A, Yasiki N, Sudaryatma PE, Goto Y, Okabayashi T
The Journal of veterinary medical science 2019年6月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本獣医学会
The incidence of diseases caused by nontuberculous mycobacteria (NTM) is increasing annually worldwide, including Japan.<i> Mycobacterium avium </i>subsp.<i> hoiminissuis </i>(MAH) is one of the most common NTM species responsible for chronic lung diseases in animals and humans. In the current study, mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing was employed to characterize the genetic diversity of swine MAH isolates from Kyushu, Japan. In total, 309 isolates were obtained from the lymph nodes of 107 pigs not displaying any clinical signs of disease, of which 307 were identified as MAH, comprising 173 strains. Based on eight established MIRU-VNTR loci, the MAH strains represented 50 genotypes constituting three lineages, and 29 had not been described in the Mac French National Institute for Agricultural Research Nouzilly MIRU-VNTR (Mac-INVM) database. MAH was the dominant <i>M. avium</i> complex (MAC) in pigs from Kyushu, and there was high genetic diversity among genotype profiles of MAH from Kyushu. We identified three predominant genotype profiles in the tested area sharing high relatedness with genotype profiles of strains isolated in European countries. MAH was the most common NTM in pigs from Kyushu and exhibited high diversity, with new strain-derived genotypes.
DOI： 10.1292/jvms.19-0048
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Kosoltanapiwat N., Reamtong O., Okabayashi T., Ampawong S., Rungruengkitkun A., Thiangtrongjit T., Thippornchai N., Leaungwutiwong P., Mahittikorn A., Mori H., Yoohanngoa T., Yamwong P.
BMC Microbiology 18 ( 1 ) 135 2018年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：BMC Microbiology
© 2018 The Author(s). Background: The pteropine orthoreovirus (PRV) was isolated from monkey (Macaca fascicularis) faecal samples collected from human-inhabited areas in Lopburi Province, Thailand. These samples were initially obtained to survey for the presence of hepatitis E virus (HEV). Results: Two virus isolates were retrieved by virus culture of 55 monkey faecal samples. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was successfully used to identify the viruses as the segmented dsRNA orthoreovirus. Phylogenetic analysis of the Lopburi orthoreovirus whole-genomes revealed relationships with the well-characterised PRVs Pulau (segment L1), Cangyuan (segments L2, M3 and S3), Melaka (segments L3 and M2), Kampar (segments M1 and S2) and Sikamat (segments S1 and S4) of Southeast Asia and China with nucleotide sequence identities of 93.5-98.9%. RT-PCR showed that PRV was detected in 10.9% (6/55) and HEV was detected in 25.5% (14/55) of the monkey faecal samples. Conclusions: PRV was isolated from monkey faeces for the first time in Thailand via viral culture and LC-MS/MS. The genetic diversity of the virus genome segments suggested a re-assortment within the PRV species group. The overall findings emphasise that monkey faeces can be sources of zoonotic viruses, including PRV and HEV, and suggest the need for active virus surveillance in areas of human and monkey co-habitation to prevent and control emerging zoonotic diseases in the future.
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Koike N., Mai TN., Shirai M., Kubo M., Hata K., Marumoto N., Watanabe S., Sasaki Y., Mitoma S., Notsu K., Okabayashi T., Wiratsubaki A. ,Kabali E., Norimine J., Sekiguchi S.
Journal of Veterinary Medical Science 80 ( 11 ) 1782 - 1786 2018年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
The purpose of this study was to detect porcine epidemic diarrhea virus (PEDV) subclinically infected pigs shipped from non-case farms to slaughterhouses. Systematic sampling was conducted at two slaughterhouses. A total of 1,556 blood samples were collected from 80 case and non-case farms from pigs over 6 months old. Blood samples were centrifuged to obtain sera. Serial serum dilutions were subjected to serological examination for PEDV presence using Neutralization test (NT). The cut-off titer was set at titer of 1:2 dilution and farms with at least one positive sample in duplicate were classified as PED-positive farms. Several non-case farms (9.4%, 6/64) and 100% (16/16) of the case farms were indeed positive for PEDV. The proportion of seropositive animals from case farms was 63.7%, significantly different from that of non-case farms (4.3%, <i>P</i><0.05). In both case and non-case farms, the proportion of seropositive animals in farrow-to-finish farms was significantly higher than in wean-to-finish farms (<i>P</i><0.05). Seropositive animals in non-case farms were detected by NT in a sero-survey by sampling at slaughterhouses. Therefore, subclinically infected pigs should be considered prior to shipment.
DOI： 10.1292/jvms.18-0132
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Sudaryatma P., Nakamura K., Mekata H., Sekiguchi S., Kubo M., Kobayashi I., Subangkit M., Goto Y., Okabayashi T.
Veterinary Microbiology 220 33 - 38 2018年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Veterinary Microbiology
© 2018 Elsevier B.V. Primary infection with bovine respiratory syncytial virus (BRSV) predisposes cattle to secondary infection with bacteria that cause bovine respiratory disease complex (BRDC). However, the interaction between BRSV and bacteria is unclear. This in vitro study examined the adherence of Pasteurella multocida (PM) to BRSV-infected cells was assessed in colony forming unit assays, by flow cytometry analysis, and by indirect immunofluorescence analysis (IFA) of epithelial cells (A549, HEp-2, and MDBK). An in vitro model based on infection of BRSV-infected epithelial cells revealed that PM adherence to BRSV-infected cells was 2- to 8-fold higher than uninfected cells. This was confirmed by flow cytometry analysis and IFA. Epithelial cell expression of mRNA encoding cytokines and chemokines increased after exposure to PM, but increased further after co-infection with BRSV and PM. BRSV-mediated adherence of PM to epithelial cells may underlie the serious symptoms of BRDC.
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Mai T., Nguyen V., Yamazaki W., Okabayashi T., Mitoma S., Notsu K., Sakai Y., Yamaguchi R., Norimine J., Sekiguchi S.
BMC Veterinary Research 14 ( 1 ) 172 2018年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：BMC Veterinary Research
© 2018 The Author(s). Background: Porcine epidemic diarrhoea (PED) is an emerging disease in pigs that causes massive economic losses in the swine industry, with high mortality in suckling piglets. Early identification of PED virus (PEDV)-infected herd through surveillance or monitoring strategies is necessary for mass control of PED. However, a common working diagnosis system involves identifying PEDV-infected animals individually, which is a costly and time-consuming approach. Given the above information, the thrusts of this study were to develop a real-time fluorescent reverse transcription loop-mediated isothermal amplification (RtF-RT-LAMP) assay and establish a pooled testing system using faecal sample to identify PEDV-infected herd. Results: In this study, we developed an accurate, rapid, cost-effective, and simple RtF- RT-LAMP assay for detecting the PEDV genome targeting M gene. The pooled testing system using the RtF-RT-LAMP assay was optimized such that a pool of at least 15 individual faecal samples could be analysed. Conclusions: The developed RtF-RT-LAMP assay in our study could support the design and implementation of large-scaled epidemiological surveys as well as active surveillance and monitoring programs for effective control of PED.
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Jain J., Okabayashi T., Kaur N., Nakayama E., Shioda T., Gaind R., Kurosu T., Sunil S.
Virology Journal 15 ( 1 ) 84 2018年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Virology Journal
© 2018 The Author(s). Background: Chikungunya virus (CHIKV) and dengue virus (DENV) are arboviruses that share the same Aedes mosquito vector, and there is much overlap in endemic areas. In India, co-infection with both viruses is often reported. Clinical manifestations of Chikungunya fever is often confused with dengue fever because clinical symptoms of both infections are similar. It is, therefore, difficult to differentiate from those of other febrile illnesses, especially dengue fever. We previously developed a CHIKV antigen detection immunochromatography (IC) rapid diagnosis kit [1]. The current study examined the efficacy of previously mentioned IC kit in India, a dengue-endemic country. Methods: Sera from 104 CHIKV-positive (by qRT-PCR) and/or IgM-positive (ELISA) subjects collected in 2016, were examined. Fifteen samples from individuals with CHIKV-negative/DENV-positive and 4 samples from healthy individuals were also examined. Of the 104 CHIKV-positive sera, 20 were co-infected with DENV. Results: The sensitivity, specificity and overall agreement of the IC assay were 93.7, 95.5 and 94.3%, respectively, using qRT-PCR as a gold standard. Also, there was a strong, statistically significant positive correlation between the IC kit device score and the CHIKV RNA copy number. The IC kit detected CHIKV antigen even in DENV-co-infected patient sera and did not cross-react with DENV NS1-positive/CHIKV-negative samples. Conclusions: The results suggest that the IC kit is useful for rapid diagnosis of CHIKV in endemic areas in which both CHIKV and DENV are circulating.
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Molecular epidemiological survey and phylogenetic analysis of bovine influenza D virus in Japan 査読あり
Mekata H., Yamamoto M., Hamabe S., Tanaka H., Omatsu T., Mizutani T., Hause B., Okabayashi T.
Transboundary and Emerging Diseases 65 ( 2 ) e355 - e360 2018年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Transboundary and Emerging Diseases
© 2017 Blackwell Verlag GmbH The influenza D virus, a new member of the Orthomyxoviridae family, is predominantly found in cattle. Although viral pathology and clinical disease in cattle appear mild, this virus plays an important role as a trigger of bovine respiratory disease (BRD). BRD is a costly illness worldwide. Thus, epidemiological surveys of the influenza D virus are necessary. Here, we conducted a molecular epidemiological survey for the influenza D virus in healthy and respiratory-diseased cattle in Japan. We found that 2.1% (8/377) of the cattle were infected with influenza D. The cattle with and without respiratory symptoms had approximately equal amounts of the virus. A full-genome sequence analysis revealed that the influenza D virus that was isolated in Japan formed an individual cluster that was distinct from the strains found in other countries. These results suggest that this virus might have evolved uniquely in Japan over a long period of time and that the viral pathology of Japanese strains might be different from the strains found in other countries. Continuous surveillance is required to determine the importance of this virus and to characterize its evolution.
DOI： 10.1111/tbed.12765
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KOIKE Naoki, NOTSU Kosuke, OKABAYASHI Tamaki, WIRATSUDAKUL Anuwat, KABALI Emmanuel, NORIMINE Junzo, SEKIGUCHI Satoshi, MAI Thi Ngan, SHIRAI Mamoru, KUBO Meiko, HATA Kazuhiro, MARUMOTO Nobuyuki, WATANABE Shinji, SASAKI Yosuke, MITOMA Shuya
Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 80 ( 11 ) 1782 - 1786 2018年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人 日本獣医学会
The purpose of this study was to detect porcine epidemic diarrhea virus (PEDV) subclinically infected pigs shipped from non-case farms to slaughterhouses. Systematic sampling was conducted at two slaughterhouses. A total of 1,556 blood samples were collected from 80 case and non-case farms from pigs over 6 months old. Blood samples were centrifuged to obtain sera. Serial serum dilutions were subjected to serological examination for PEDV presence using Neutralization test (NT). The cut-off titer was set at titer of 1:2 dilution and farms with at least one positive sample in duplicate were classified as PED-positive farms. Several non-case farms (9.4%, 6/64) and 100% (16/16) of the case farms were indeed positive for PEDV. The proportion of seropositive animals from case farms was 63.7%, significantly different from that of non-case farms (4.3%, <i>P</i><0.05). In both case and non-case farms, the proportion of seropositive animals in farrow-to-finish farms was significantly higher than in wean-to-finish farms (<i>P</i><0.05). Seropositive animals in non-case farms were detected by NT in a sero-survey by sampling at slaughterhouses. Therefore, subclinically infected pigs should be considered prior to shipment.
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Rahpaya S., Tsuchiaka S., Kishimoto M., Oba M., Katayama Y., Nunomura Y., Kokawa S., Kimura T., Kobayashi A., Kirino Y., Okabayashi T., Nonaka N., Mekata H., Aoki H., Shiokawa M., Umetsu M., Morita T., Hasebe A., Otsu K., Asai T., Yamaguchi T., Makino S., Murata Y., Abi A., Omatsu T., Mizutani T.
Journal of Veterinary Science 19 ( 3 ) 350 - 357 2018年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Veterinary Science
© 2018 The Korean Society of Veterinary Science. Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain reaction (PCR), which is based on TaqMan real-time PCR. Fifty-seven samples were positive for at least one pathogen, including bovine viral diarrhea virus, bovine enterovirus, Salmonella enterica ser. Dublin, Salmonella enterica ser. Typhimurium, and Neospora caninum; some samples were positive for multiple pathogens. Bovine viral diarrhea virus and bovine enterovirus were the most frequently detected pathogens, especially in flies, suggesting an important role of flies in the transmission of these viruses. Additionally, we detected the N. caninum genome from a cockroach sample for the first time. Our data suggest that insects (particularly flies), birds, and rodents are potential vectors and reservoirs of abortion, diarrhea, and respiratory infectious agents, and that they may transmit more than one pathogen at the same time.
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Dengue Virus-Induced Reactive Oxygen Species Production in Rat Microglial Cells. 査読あり
Suwanprinya L, Phumala Morales N, Sanvarinda P, Dieng H, Okabayashi T, Enrique Morales Vargas R
Japanese journal of infectious diseases 70 ( 4 ) 383 - 387 2017年7月
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Diagnostic accuracy of a rapid E1-antigen test for chikungunya virus infection in a reference setting. 査読あり
Huits R, Okabayashi T, Cnops L, Barbé B, Van Den Berg R, Bartholomeeusen K, Ariën KK, Jacobs J, Bottieau E, Nakayama EE, Shioda T, Van Esbroeck M
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases 2017年6月
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Sabike, I., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Okabayashi, T., Goto, Y., Yamazaki, W.
Frontiers in Microbiology 7 ( SEP ) 1582 2016年9月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Frontiers in Microbiology
© 2016 Sabike, Uemura, Kirino, Mekata, Sekiguchi, Okabayashi, Goto and Yamazaki. Rapid identification of Campylobacter-positive flocks before slaughter, following freezing and heat treatment for the Campylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. We evaluated a loop-mediated isothermal amplification (LAMP) assay for the direct screening of naturally contaminated chicken cloacal swabs for C. jejuni/C. coli to compare this assay with conventional qu antitative culture methods. In a comparison study of 165 broilers, the LAMP assay showed 82.8% (48/58 by conventional culture) sensitivity, 100% (107/107) specificity, 100% (48/48) positive predictive value (PPV), and 91.5% (107/117) negative predictive value (NPV). In a comparison of 55 flocks, LAMP showed 90.5% (19/21) sensitivity, 100% (34/34) specificity, 100% (19/19) PPV, and 94.4% (34/36) NPV. In the cumulative total of 28 farm-level comparisons, LAMP showed 100% (12/12) sensitivity, 100% (16/16) specificity, 100% (12/12) PPV, and 100% (16/16) NPV. The LAMP assay required less than 90 min from the arrival of the fecal samples to final results in the laboratory. This suggests that the LAMP assay will facilitate the identification of C. jejuni/C. coli-positive broiler flocks at the farm level or in slaughterhouses before slaughtering, which would make it an effective tool in preventing the spread of Campylobacter contamination.
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Dengue virus infection-enhancing antibody activities against Indonesian strains in inhabitants of central Thailand.
Yamanaka, A., Oddgun, D., Chantawat, N., Okabayashi, T., Ramasoota, P., Churrotin, S., Kotaki, T., Kameoka, M., Soegijanto, S., Konishi, E.
Microbes and infection 18 ( 4 ) 277 - 84 2016年4月
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Correction for Okabayashi et al., Detection of Chikungunya Virus Antigen by a Novel Rapid Immunochromatographic Test.
Okabayash,i T., Sasaki, T., Masrinoul, P., Chantawat, N., Yoksan, S., Nitatpattana, N., Chusri, S., Morales, Vargas. RE, Grandadam, M., Brey, PT., Soegijanto, S., Mulyantno, KC, Churrotin S, Kotaki, T., Faye, O., Faye, O., Sow, A., Sall, AA., Puiprom, O., Chaichana P., Kurosu, T., Kato, S., Kosaka, M., Ramasoota, P., Ikuta, K.
Journal of clinical microbiology 54 ( 4 ) 1173 - 4 2016年4月
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Detection of chikungunya virus antigen by a novel rapid immunochromatographic test.
Okabayashi T, Sasaki T, Masrinoul P, Chantawat N, Yoksan S, Nitatpattana N, Chusri S, Morales Vargas RE, Grandadam M, Brey PT, Soegijanto S, Mulyantno KC, Churrotin S, Kotaki T, Faye O, Faye O, Sow A, Sall AA, Puiprom O, Chaichana P, Kurosu T, Kato S, Kosaka M, Ramasoota P, Ikuta K
Journal of clinical microbiology 53 ( 2 ) 382 - 8 2015年2月
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Chikungunya virus was isolated in Thailand, 2010.
Sasayama M, Benjathummarak S, Kawashita N, Rukmanee P, Sangmukdanun S, Masrinoul P, Pitaksajjakul P, Puiprom O, Wuthisen P, Kurosu T, Chaichana P, Maneekan P, Ikuta K, Ramasoota P, Okabayashi T, Singhasivanon P, Luplertlop N
Virus genes 49 ( 3 ) 485 - 9 2014年12月
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Monoclonal antibody targeting chikungunya virus envelope 1 protein inhibits virus release.
Masrinoul P, Puiprom O, Tanaka A, Kuwahara M, Chaichana P, Ikuta K, Ramasoota P, Okabayashi T
Virology 464-465 111 - 7 2014年9月
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Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus.
Pitaksajjakul P, Benjathummarak S, Pipattanaboon C, Wongwit W, Okabayashi T, Kuhara M, Misaki R, Fujiyama K, Ramasoota P
Biochemical and biophysical research communications 446 ( 2 ) 475 - 80 2014年4月