Details of a Researcher - KANEKO Masatoki

Low maternal immunoglobulin g avidity and single parity as adverse implications of human cytomegalovirus vertical transmission in pregnant women with immunoglobulin m positivity Reviewed

The estimation of a cytomegalovirus immunoglobulin G avidity using the mathematical model Invited Reviewed

Masatoki Kaneko

Journal of Basic and Clinical Pharmacy 13 ( 127 ) 127 2022.3

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.37532/0976-0113.13(1).127

Kaneko M., Muraoka J., Kusumoto K., Minematsu T.

Viruses 13 ( 5 ) 2021.5

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Viruses

Human cytomegalovirus (CMV) is the leading cause of neurological sequelae in infants. Understanding the risk factors of primary CMV infection is crucial in establishing preventive strategies. Thus, we conducted a retrospective cohort study to identify risk factors of vertical transmission among pregnant women with immunoglobulin (Ig) M positivity. The study included 456 pregnant women with IgM positivity. Information on age, parity, occupation, clinical signs, IgM levels, and IgG avidity index (AI) was collected. The women were divided into infected and non-infected groups. The two groups showed significant differences in IgM level, IgG AI, number of women with low IgG AI, clinical signs, and number of pregnant women with single parity. In the multiple logistic regression analysis, pregnant women with single parity and low IgG AI were independent predictors. Among 40 women who tested negative for IgG antibody in their previous pregnancy, 20 showed low IgG AI in their current pregnancy. Among the 20 women, 4 had vertical transmission. These results provide better understanding of the risk factors of vertical transmission in pregnant women with IgM positivity.

DOI： 10.3390/v13050866

A multiple regression model for predicting a high cytomegalovirus immunoglobulin G avidity level in pregnant women with IgM positivity Reviewed

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Kaneko M., Ohhashi M., Fujii Y., Minematsu T., Kusumoto K.

International Journal of Infectious Diseases 100 1 - 6 2020.11

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：International Journal of Infectious Diseases

Objective: To establish a model to predict high cytomegalovirus (CMV) immunoglobulin (Ig)G avidity index (AI) using clinical information, to contribute to the mental health of CMV-IgM positive pregnant women. Methods: We studied 371 women with IgM positivity at ≤14 w of gestation. Information on the age, parity, occupation, clinical signs, IgM and G values, and IgG AI was collected. The IgG AI cut-off value for diagnosing congenital infection was calculated based on a receiver operating characteristic curve analysis. Between-group differences were assessed using the Mann–Whitney U-test or χ2 analysis. The factors predicting a high IgG AI were determined using multiple logistic regression. Results: The women were divided into high or low IgG AI groups based on an IgG AI cut-off value of 31.75. There were significant differences in the IgG and IgM levels, age, clinical signs, and the number of women with one parity between the two groups. In a multiple logistic regression analysis, IgM and the number of women with one parity were independent predictors. This result helped us establish a mathematical model that correctly classified the IgG AI level for 84.6% of women. Conclusion: We established a highly effective model for predicting a high IgG AI immediately after demonstrating IgM positivity.

DOI： 10.1016/j.ijid.2020.08.034

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Intrapartum fetal heart rate monitoring in cases of cytomegalovirus infection Reviewed

金子　政時,鮫島　浩,池田　智明,池ノ上　克,峰松　俊夫

Am. J. Obstet. Gynecol. 191 1257 - 1262 2014.9

Language：English Publishing type：Research paper (scientific journal)

特集ローリスク妊婦ローリスク新生児のケア2025 妊娠時妊婦健診における検体検査 Reviewed

金子政時, 谷口光代

周産期医学 55 ( 1 ) 32 - 34 2025.1

Publishing type：Research paper (scientific journal) Publisher：東京医学社

DOI： 10.24479/peri.0000001987

Effect of domestic violence on the health-related quality of life of pregnant women during the COVID-19 pandemic Reviewed

Honoka Tsuda, Masatoki Kaneko, Kurumi Tsuruta, Keiko Yamazaki, Ayako Tanabe, Saori Yoshinaga, Mitsuyo Taniguchi, Yoshinori Fujii

Journal of Japan Health Medicine Association 33 ( 2 ) 200 - 207 2024.7

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

Impact of COVID-19 on Cytomegalovirus Immunoglobulin M Antibody Index Reviewed

Kaneko M., Muraoka J., Yang L., Tokunaga S., Minematsu T.

Clinical and Experimental Obstetrics and Gynecology 50 ( 12 ) 2023.12

Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Clinical and Experimental Obstetrics and Gynecology

Background: Coronavirus disease 2019 (COVID-19) influenced the prevalence of other infectious diseases, including congenital cytomegalovirus (CMV) infection. However, the effect of COVID-19 on antibody titers has not been reported. This study aimed to explore the influence of COVID-19 on levels of CMV immunoglobulin M (IgM) in pregnant women. Methods: This cross-sectional study included pregnant women who visited the University Hospital due to CMV IgM positivity during the 7th and 8th waves of COVID-19. Data, including maternal characteristics, history of COVID-19, CMV immunoglobulin G (IgG) and IgM index, and IgG avidity index (AI) were collected. Chemiluminescent immunoassay was performed to measure levels of IgG and IgM. Polymerase chain reaction using neonatal urine was performed to confirm congenital infection. Results: Of the 89 pregnant women, 36 (40%) (low IgG AI: n = 10; high IgG AI: n = 26) contracted COVID-19. Among 21 women with low IgG AI, 9 (false IgM positive: n = 8; primary infection: n = 1) had an IgG AI of 0. Among the eight women with false IgM positivity, six (75%) contracted COVID-19. The IgM index of pregnant women with false IgM positivity was 12.6 ± 10.9. Meanwhile, the CMV IgM index of pregnant women with false IgM positivity in the non-COVID-19-infected group was 1.7 ± 0.5. When the IgM indices of women who contracted (n = 36) and did not contract (n = 53) COVID-19 were compared, the IgM index of infected women (4.4 ± 5.7) was higher than those of non-infected women (2.7 ± 3.0) (p = 0.01). Regarding IgM and IgG AI, multiple logistic regression analysis revealed that there were no significantly different variables between the two groups. Conclusions: High prevalence of false IgM positivity was observed among women who contracted COVID-19. The IgM index of pregnant women with false IgM positivity was high. Caution should be exercised in interpreting CMV IgM indices in pregnant women with a history of COVID-19.

DOI： 10.31083/j.ceog5012279

特集数値からみる周産期医療産科編先天性感染症 Reviewed

金子政時

周産期医学 53 ( 8 ) 1214 - 1216 2023.8

Publishing type：Research paper (scientific journal) Publisher：東京医学社

DOI： 10.24479/peri.0000001051

Cell-mediated and humoral immune responses to human cytomegalovirus in pregnant women with vertically transmitted infection following primary infection: A case report Reviewed

Yamada N., Kaneko M., Yang L., Matsuzawa S., Minematsu T., Kodama Y.

Journal of Infection and Chemotherapy 29 1071 - 1074 2023.7

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Infection and Chemotherapy

Human cytomegalovirus (HCMV) is the major cause of neurological sequelae in infants. Immune control of primary HCMV infection appears to depend on the interaction between humoral and cell-mediated immune responses. We report the case of an HCMV-transmitter mother observed with dissociation between humoral and cell-mediated immune responses. The patient had immunoglobulin (Ig) G and M positivity at 11 weeks of gestation and showed fetal hyperechoic bowel and minimal ascites at 21 weeks of gestation. At 25 weeks of gestation, the polymerase chain reaction result for HCMV using amniotic fluid was positive. The numbers of spots in the enzyme-linked immunosorbent spot (ELISPOT) assay at 25, 36, and 39 weeks of gestation were three, five, and six spots/2 × 105 peripheral blood mononuclear cells, respectively. Furthermore, IgG avidity indexes (AIs) at 21, 25, 36, and 39 weeks of gestation were 37.6, 49.7, 72.5, and 74.3, respectively. At 40+1 weeks of gestation, the patient delivered a symptomatic infected newborn with a weight of 2,384 g (−2.6 SD) and a head circumference of 30 cm (−2.6 SD). The neonate had a petechial rash and bilateral hearing loss although did not show liver dysfunction or thrombocytopenia. Cranial magnetic resonance imaging revealed mild ventriculomegaly, left lateral/parietal polymicrogyria, and a punctate white matter lesion. This case showed that IgG AI increased with increasing gestational age, whereas the numbers of spots in the ELISPOT assay had no change. The dissociation between humoral and cell-mediated immune responses may be characteristic of the immune response of a transmitter mother.

DOI： 10.1016/j.jiac.2023.07.004

Work environment to achieve work-life balance in workers raising children: A questionnaire survey of young workers in Miyazaki City Reviewed

Tanabe Ayako, Hisadome Aoi, Yamamoto Asuka, Suetsugu Norie, Kaneko Masatoki

The South Kyusyu journal of nursing 21 ( 1 ) 1 - 8 2023.3

Language：Japanese Publishing type：Research paper (scientific journal)

Prevalence of congenital cytomegalovirus infection according to the type of maternal infection in Japan Reviewed

Work environment to achieve work-life balance in workers raising children: A questionnaire survey of young in Miyazaki Cityy Reviewed

21 ( 1 ) 1 - 8 2023.3

Authorship：Corresponding author Language：Japanese Publishing type：Research paper (bulletin of university, research institution)

A qualitative study of maternal and child health coordinators on mothers' concerns Reviewed

kaho Yamshita, Keiko Yamazaki, Ayaka Matsuoka, Masatoki Kaneko

20 ( 1 ) 9 - 16 2023.3

Authorship：Corresponding author Language：Japanese Publishing type：Research paper (bulletin of university, research institution)

Kaneko M., Yang L., Tanabe A., Fujii Y., Nakao H., Minematsu T.

Journal of Infection and Chemotherapy 29 485 - 489 2023

Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Infection and Chemotherapy

Introduction: Variable rates of cytomegalovirus (CMV) seropositivity in mothers from different individual's background may translate to distinct epidemiological patterns of congenital CMV infection. Methods: The prospective cohort study was conducted in Japan to evaluate the prevalence of vertical transmission rate according to the type of maternal infection. Post hoc power as a follow-up analysis was evaluated to compare the statistical power with other studies from France, Finland and Brazil. One thousand one hundred sixty-three pregnant women were measured IgG, IgM and IgG avidity index. The urine samples of neonates of these women were evaluated using polymerase chain reaction to diagnose the vertical transmission. Results: The prevalence of congenital CMV infection in the study population was 0.4%. The proportions of patients with primary and nonprimary infections were 60% and 40%, respectively, with a maternal seroprevalence of 82.5%. The rate of vertical transmission among the seronegative pregnant women before pregnancy was statistically higher than that among the seropositive pregnant women before pregnancy (p < 0.05), with a study power of 52.7%. The same difference was observed in France and Finland for maternal seroprevalence of 61% and 72% and statistical power of 56.9% and 66.7%, respectively. Conclusion: The maternal seroprevalence of the present study conducted in Japan was much higher than that of studies in France and Finland. Nevertheless, seronegative pregnant women had a higher risk of vertical transmission before pregnancy.

DOI： 10.1016/j.jiac.2023.01.020

Pulmonary lymphangiectasia in an extremely preterm infant complicated with <i>Ureaplasma</i> pneumonitis Reviewed

Sakakibara Kouhei, Yamada Naoshi, Kodama Yuki, Obata Shizuka, Tsuzuki Yasue, Muraoka Junsuke, Aoki Yoshinori, Yamashita Rie, Nakame Kazuhiko, Kaneko Masatoki, Katsuragi Shinji, Tsuzuki Ryo, Sato Yuichiro

Journal of Japan Society of Perinatal and Neonatal Medicine 59 ( 1 ) 116 - 121 2023

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society of Perinatal and Neonatal Medicine

Here we report a case of pulmonary lymphangiectasia that caused exacerbation of respiratory status after an infection. A preterm infant born at 23 weeks of gestation without obvious clinical intrauterine infection showed worsening respiratory status and increased c-reactive protein on day 15 of life. Chest X-ray showed consolidation in right upper lobe and whole left lung. Several broad-spectrum antibiotics were administered unsuccessfully. Finally, <i>Ureaplasma urealyticum</i> was detected in sputum culture, and azithromycin was administered. Although the inﬂammation improved, tension pneumothorax and pulmonary hypertension developed and he died on day 44 of life. At autopsy, there were few ﬁndings of lung inﬂammation, and congenital lymphangiectasia was diagnosed. It seems that exacerbation was triggered by the infection, although there was no remarkable evidence to support causality. There have been few case reports of pulmonary lymphangiectasia in extremely low birth weight infants. This case was considered to be classiﬁed as primary, although the respiratory condition at birth was relatively good. There are no clear diagnostic criteria for this disease and no eﬀective treatments. Clinically, neonatologists should keep in mind pulmonary lymphangiectasia if respiratory failure in newborns does not respond to common treatment approach.

DOI： 10.34456/jjspnm.59.1_116

先天性感染症

金子政時

周産期医学 53 1214 - 1216 2023

Publishing type：Research paper (scientific journal)

増刊号 191の疑問に答える周産期の栄養産科編Q & A 妊娠中期 Q80 食後の胸焼けがひどくなりました。　食事はどうしたらよいですか?　薬はありますか? Reviewed

金子政時, 前山彩, 松岡あやか

周産期医学 52 ( 13 ) 179 - 180 2022.11

Publishing type：Research paper (scientific journal) Publisher：東京医学社

DOI： 10.24479/peri.0000000490

Antepartum Antibiotic Therapy under 34 Weeks of Gestation and Its Impact on Early-Onset Neonatal Infection and Maternal Vaginal Microbiota Reviewed

Muraoka J., Kaneko M., Doi K., Kodama Y., Sameshima H.

Microbiology Research 13 ( 3 ) 598 - 608 2022.9

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Microbiology Research

The use of prenatal antibiotics should be carefully considered, owing to their potential adverse effects on neonatal outcomes. This study aimed to identify the contributing factors to early-onset neonatal infection and to determine the influence of antepartum antibiotics on women and neonates. This study included 127 pregnant women without obvious intra-amniotic infection on admission, who delivered under 34 weeks of gestation. Information on maternal and neonatal characteristics was obtained from their medical charts. Vaginal swabs were taken from all women on admission. In total, 29 (22.8%) neonates developed early-onset infection. Multivariate analysis revealed that antepartum antibiotics were the most strongly associated factor for early-onset neonatal infection (odds ratio, 11.2; 95% confidence interval, 4.08–31.02). The frequency of early-onset neonatal infection was significantly higher in women who received antibiotic therapy than in those who did not; no significant difference in prolonging their gestation or neonatal morbidities was observed. The prevalence of women who hosted vaginal microorganisms on admission was similar to that in women whose infants subsequently developed early-onset neonatal infection compared with that of women whose infants did not. Among infants of the 40 women who received antepartum antibiotic therapy, 21 developed early-onset infection. Of the women who delivered these 21 infants, 62% (13/21) showed reduced lactobacilli and 43% (9/21) had resistant bacterial strains in their vaginal microbiota at the time of delivery. The use of antepartum antibiotics is the most strongly associated factor in early-onset neonatal infection; it does not prolong gestation and would change the vaginal environment.

DOI： 10.3390/microbiolres13030042