論文 - 榊原 陽一
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Uchida A., Takenaka S., Takenaka S., Sakakibara Y., Kurogi S., Honsho C., Sassa H., Suiko M., Kunitake H.
Journal of the Japanese Society for Horticultural Science 81 ( 2 ) 150 - 158 2012年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of the Japanese Society for Horticultural Science
In Citrus, self-incompatibility (SI) regulation is gametophytic, and this phenomenon is an economically critical problem for some Citrus cultivars without high parthenocarpic ability. Few molecular biological studies of SI in Citrus have been performed, and the molecular mechanism of SI has not been clarified. To investigate the effect of different stages of style development on pollen tube behavior, flower buds of self-incompatible 'Hyuganatsu' (Citrus tamurana hort. ex Tanaka) were histologically assayed. When the flower buds were observed 168 hours after pollination, pollen tubes in the self-pollinated flower buds 1 and 3 days before anthesis (DBA) were arrested in the upper part of the styles, while those in flower buds self-pollinated 5 DBA reached the base of the styles. These results revealed that SI in 'Hyuganatsu' has not yet occurred in the flower buds 5 DBA, but generated in the flower buds 3 DBA. To search SI-related pistil proteins in Citrus, we profiled a number of protein expressions in 'Hyuganatsu' styles of 1, 3, and 5 DBA by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. One-hundred thirty-eight protein spots were significantly different in abundance among the three stages, and 17 up-regulated and 26 down-regulated protein spots could be identified. Among the 17 up-regulated proteins, nine up-regulated proteins exhibited the expression pattern of 1 DBA ≥ 3 DBA > 5 DBA, a pattern which reflected the transmission from SC to SI, evaluated by pollen tube growth. BLASTP homology search against the peptide sequences of these proteins was carried out and predicted the proteins related to the maintenance of cell shape, the signaling of pollen tube growth, the flavonol biosynthesis, the responses to various stresses, photosynthesis, and the methionine metabolic process. Among the nine proteins, some may be the SI-related pistil proteins; however, it was not possible to determine the SI-related key protein of the style in Citrus. © 2012.
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Alpha Lipoic Acid Selectively Inhibits Proliferation and Adhesion to Fibronectin of v-H-ras-transformed 3Y1 Cells. 査読あり
Yamasaki, M., Iwase, M., Kawano, K., Sakakibara, Y., Suiko, M., and K. Nishiyama.
J. Clin. Biochem. Nutr 50 234 - 240 2012年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Conjugated linoleic acids inhibit hypoxia inducible factor-1alpha stabilization under hypoxic condition in human hepatocellular carcinoma cells. 査読あり
Yamasaki, M., Nagatomo, T., Matsuyama, T., Ikeho, Y., Kato, E., Nishiyama, K., Sakakibara, Y., Suiko, M., Nishiyama, N.
J. Oleo Sci. 61 ( 9 ) 491 - 496 2012年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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自家不和合性の'ヒュウガナツ'（<i>Citrus tamurana</i> hort. ex Tanaka）花柱の異なる発達ステージにおける発現タンパク質の網羅的解析
内田 飛香, 竹中 聡, 榊原 陽一, 黒木 重文, 本勝 千歳, 佐々 英徳, 水光 正仁, 國武 久登
Journal of the Japanese Society for Horticultural Science 81 ( 2 ) 150 - 158 2012年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：一般社団法人 園芸学会
これまでにカンキツにおける自家不和合性の分子生物学的研究が行われてきたが，そのメカニズムについては未だ明らかになっていない．花柱の成育ステージの違いが引き起こす花粉管の挙動の経時的変化を調査するため，自家不和合性品種'ヒュウガナツ'の花蕾の組織学的調査を行った．自家受粉 168 時間後において，開花 1 日前と 3 日前に受粉した花蕾の花粉管は花柱上部に停滞していた．一方，開花 5 日前に受粉した花蕾では花粉管が花柱基部まで到達していた．これらの結果，'ヒュウガナツ'の開花 5 日前の花蕾においては自家不和合性反応は生じていないが，開花 3 日前の花蕾においては自家不和合性反応が生じていることが明らかとなった．そこで，カンキツにおける自家不和合性に関連する雌ずいタンパク質を探索するため，'ヒュウガナツ'の開花 1，3，5 日前の花蕾における発現タンパク質のプロファイリングを二次元電気泳動法と MALDI-TOF/MS 法を用いて行った．3 つの異なるステージにおいて，138 個のタンパク質の発現量に有意な差が観察され，そのうち 17 個の発現量が上昇しているタンパク質と，26 個の発現量が減少しているタンパク質が同定された．17 個の発現量が上昇していたタンパク質のうち，9 個のタンパク質が示す開花 1 日前 ≧ 開花 3 日前 ＞ 開花 5 日前の発現パターンが，花粉管の挙動に基づく自家和合性から自家不和合性への移行を反映していると思われた．BLASTP によるペプチド配列の相同性探索により，これらは細胞形成の維持や花粉管伸長におけるシグナル，フラボノイド生合成系，ストレス応答，光合成系やメチオニン代謝経路に関するタンパク質であると推定された．これら 9 個のタンパク質のうち，いずれかが自家不和合性と関連を持つ花柱タンパク質である可能性があると考えられた．しかしながら，これらの中からカンキツにおける自家不和合性反応の鍵となるタンパク質を明らかにすることはできなかった．<br>
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A comparative study of the sulfation of bile acids and a bile alcohol by the Zebra danio (Danio rerio) and human cytosolic sulfotransferases (SULTs).
Kurogi K, Krasowski MD, Injeti E, Liu MY, Williams FE, Sakakibara Y, Suiko M, Liu MC.
J. Steroid Biochem. Mol. Biol. 127 ( 3-5 ) 307 - 314 2011年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Kurogi K., Krasowski M., Injeti E., Liu M., Williams F., Sakakibara Y., Suiko M., Liu M.
Journal of Steroid Biochemistry and Molecular Biology 127 ( 3-5 ) 307 - 314 2011年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Steroid Biochemistry and Molecular Biology
The current study was designed to examine the sulfation of bile acids and bile alcohols by the Zebra danio (Danio rerio) SULTs in comparison with human SULTs. A systematic analysis using the fifteen Zebra danio SULTs revealed that SULT3 ST2 and SULT3 ST3 were the major bile acid/alcohol-sulfating SULTs. Among the eleven human SULTs, only SULT2A1 was found to be capable of sulfating bile acids and bile alcohols. To further investigate the sulfation of bile acids and bile alcohols by the two Zebra danio SULT3 STs and the human SULT2A1, pH-dependence and kinetics of the sulfation of bile acids/alcohols were analyzed. pH-dependence experiments showed that the mechanisms underlying substrate recognition for the sulfation of lithocholic acid (a bile acid) and 5α-petromyzonol (a bile alcohol) differed between the human SULT2A1 and the Zebra danio SULT3 ST2 and ST3. Kinetic analysis indicated that both the two Zebra danio SULT3 STs preferred petromyzonol as substrate compared to bile acids. In contrast, the human SULT2A1 was more catalytically efficient toward lithocholic acid than petromyzonol. Collectively, the results imply that the Zebra danio and human SULTs have evolved to serve for the sulfation of, respectively, bile alcohols and bile acids, matching the cholanoid profile in these two vertebrate species. © 2010 Elsevier Ltd. All rights reserved.
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Enzymatic sulfation of tocopherols and tocopherol metabolites by human cytosolic sulfotransferases 査読あり
Hashiguchi T., Hashiguchi T., Kurogi K., Kurogi K., Sakakibara Y., Sakakibara Y., Yamasaki M., Yamasaki M., Nishiyama K., Yasuda S., Liu M., Suiko M., Suiko M.
Bioscience, Biotechnology and Biochemistry 75 ( 10 ) 1951 - 1956 2011年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Tocopherols are essential micronutrients for mammals widely known as potent lipid-soluble antioxidants that are present in cell membranes. Recent studies have demonstrated that most of the carboxychromanol (CEHC), a tocopherol metabolite, in the plasma exists primarily in sulfate- and glucuronide- conjugated forms. To gain insight into the enzymatic sulfation of tocopherols and their metabolites, a systematic investigation was performed using all 14 known human cytosolic sulfotransferases (SULTs). The results showed that the members of the SULT1 family displayed stronger sulfating activities toward tocopherols and their metabolites. These enzymes showed a substrate preference for γ-tocopherol over α-tocopherol and for γ-CEHC over other CEHCs. Using A549 human lung epithelial cells in a metabolic labeling study, a similar trend in the sulfation of tocopherols and CEHCs was observed. Collectively, the results obtained indicate that SULTmediated enzymatic sulfation of tocopherols and their metabolites is a significant pathway for regulation of the homeostasis and physiological functions of these important compounds.
DOI： 10.1271/bbb.110352
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プロテオーム解析による食品機能の研究(<特集>食の機能・安全を科学する-おいしさから生理活性,安全性の評価まで)
榊原 陽一, 中原 幸太, 森永 浩通, 山崎 正夫, 西山 和夫, 水光 正仁
生物工学会誌 : seibutsu-kogaku kaishi 89 ( 10 ) 593 - 596 2011年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人日本生物工学会
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食品成分の網羅的な機能性評価法(<特集>食の機能・安全を科学する-おいしさから生理活性,安全性の評価まで)
江藤 望, 山森 一人, 永濱 清子, 榊原 陽一, 西山 和夫, 吉原 郁夫, 水光 正仁
生物工学会誌 : seibutsu-kogaku kaishi 89 ( 10 ) 589 - 592 2011年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：公益社団法人日本生物工学会
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Efficient Approach for Simultaneous Estimation of the Multiple Health-Promoting Effects of Foods 査読あり
Nagahama, Kiyoko; Eto, Nozomu; Yamamori, Kunihito; Nishiyama, Kazuo; Sakakibara, Yoichi; Iwata, Takako; Uchida, Asuka; Yoshihara, Ikuo; Suiko, Masahito
Journal of Agricultural and Food Chemistry 59 ( 16 ) 8575 - 8588 2011年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Efficient approach for simultaneous estimation of multiple health-promoting effects of foods 査読あり
Nagahama K., Eto N., Eto N., Yamamori K., Nishiyama K., Sakakibara Y., Sakakibara Y., Iwata T., Iwata T., Uchida A., Uchida A., Yoshihara I., Suiko M., Suiko M.
Journal of Agricultural and Food Chemistry 59 ( 16 ) 8575 - 8588 2011年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Agricultural and Food Chemistry
The investigation of new food constituents for purposes of disease prevention or health promotion is an area of increasing interest in food science. This paper proposes a new system that allows for simultaneous estimation of the multiple health-promoting effects of food constituents using informatics. The model utilizes expression data of intracellular marker proteins as descriptors that reply to stimulation of a constituent. To estimate three health-promoting effects, namely, cancer cell growth suppression activity, antiviral activity, and antioxidant stress activity, each model was constructed using expression data of marker proteins as input data and health-promoting effects as the output value. When prediction performances of three types of mathematical models constructed by simple, multiple regressions, or artificial neural network (ANN), were compared, the most adequate model was the one constructed using an ANN. There were no statistically significant differences between the actual data and estimated values calculated by the ANN models. This system was able to simultaneously estimate health-promoting effects with reasonable precision from the same expression data of marker proteins. This novel system should prove to be an interesting platform for evaluation of the health-promoting effects of food. © 2011 American Chemical Society.
DOI： 10.1021/jf201836g
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Enzymatic Sulfation of Tocopherols and Tocopherol Metabolites by Human Cytosolic Sulfotransferases. 査読あり
Hashiguchi, T., Kurogi, K., Sakakibara, Y.ほか
Bioscience, Biotechnology, and Biochemistry 75 ( 10 ) 1951 - 1956 2011年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Identification of self-incompatibility related proteins in the pistil of Japanese pear (Pyrus pyrifolia (Burm.f.)) by proteome analysis 査読あり
Uchida, A., Sassa, H., Takenaka, S., Sakakibara, Y., Suiko, M., Kunitake, H.
Plant Omics J. 2011年8月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Inhibition of proliferation by agricultural plant extracts in seven human adult T-cell leukaemia (ATL)-related cell lines. 査読あり
Kai H, Akamatsu E, Torii E, Kodama H, Yukizaki C, Sakakibara Y, Suiko M, Morishita K, Kataoka H, Matsuno K.
J. Nat. Med. 65 ( 3-4 ) 651 - 655 2011年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sulfation of chlorotyrosine and nitrotyrosine by human lung endothelial and epithelial cells: Role of the human SULT1A3. 査読あり
Yasuda S, Yasuda T, Liu MY, Shetty S, Idell S, Boggaram V, Suiko M, Sakakibara Y, Fu J, Liu MC.
Toxicol. Appl. Pharmacol. 251 ( 2 ) 104 - 109 2011年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Yasuda S., Yasuda S., Yasuda T., Liu M., Shetty S., Idell S., Boggaram V., Suiko M., Sakakibara Y., Fu J., Liu M.
Toxicology and Applied Pharmacology 251 ( 2 ) 104 - 109 2011年3月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Toxicology and Applied Pharmacology
During inflammation, potent reactive oxidants formed may cause chlorination and nitration of both free and protein-bound tyrosine. In addition to serving as biomarkers of inflammation-mediated oxidative stress, elevated levels of chlorotyrosine and nitrotyrosine have been linked to the pathogenesis of lung and vascular disorders. The current study was designed to investigate whether the lung cells are equipped with mechanisms for counteracting these tyrosine derivatives. By metabolic labeling, chlorotyrosine O-[35S]sulfate and nitrotyrosine O-[35S]sulfate were found to be generated and released into the labeling media of human lung endothelial and epithelial cells labeled with [35S]sulfate in the presence of added chlorotyrosine and nitrotyrosine. Enzymatic assays using the eleven known human cytosolic sulfotransferases (SULTs) revealed SULT1A3 as the enzyme responsible for catalyzing the sulfation of chlorotyrosine and nitrotyrosine. Reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated the expression of SULT1A3 in the lung endothelial and epithelial cells used in this study. Kinetic constants of the sulfation of chlorotyrosine and nitrotyrosine by SULT1A3 were determined. Collectively, these results suggest that sulfation by SULT1A3 in lung endothelial and epithelial cells may play a role in the inactivation and/or disposal of excess chlorotyrosine and nitrotyrosine generated during inflammation. © 2010 Elsevier Inc.
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Identification, characterization, and ontogenic study of a catechol O-methyltransferase from zebrafish. 査読あり
Alazizi A, Liu MY, Williams FE, Kurogi K, Sakakibara Y, Suiko M, Liu MC.
Aquat. Toxicol. 102 ( 1-2 ) 18 - 23 2011年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Alazizi A., Liu M., Williams F., Kurogi K., Kurogi K., Sakakibara Y., Suiko M., Liu M.
Aquatic Toxicology 102 ( 1-2 ) 18 - 23 2011年3月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Aquatic Toxicology
To establish the zebrafish as a model for investigating the methylation pathway of drug metabolism, we embarked on the molecular cloning of the zebrafish catechol O-methyltransferase (COMT). By searching the GenBank database, a zebrafish nucleotide sequence encoding a putative COMT was identified. Based on the sequence information, we designed and synthesized oligonucleotides corresponding to its 5'- and 3'-coding regions of this zebrafish COMT. Using the first-strand cDNA reverse-transcribed from the total RNA isolated from a 3-month-old adult female zebrafish as the template, the cDNA encoding the zebrafish COMT was PCR-amplified. The recombinant zebrafish COMT protein was subsequently expressed in and purified from BL21 (DE3) Escherichia coli cells transformed with the pGEX-2TK expression vector harboring the zebrafish COMT cDNA. Upon enzymatic characterization, purified COMT displayed methylating activity toward dopamine, dopa, and catecholestrogens, as well as three representative catechol drugs, methyldopa, dobutamine, and isoproterenol. A reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed developmental stage-dependent expression of the zebrafish COMT during embryonic development and throughout the larval stage onto maturity. These results provide a foundation for investigating the involvement of COMT-mediated methylation in protection against the adverse effects of catechol drugs and other xenobiotic catechols during the developmental process. © 2010 Elsevier B.V.
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中国餅麹(曲)由来の新規黒色系糸状菌の焼酎醸造特性 査読あり
野崎 直樹ほか6名
生物工学会誌 89 ( 1 ) 20 - 26 2011年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Novel screening method for potential skin-whitening compounds by a luciferase reporter assay. 査読あり
Shirasugi I, Sakakibara Y, Yamasaki M, Nishiyama K, Matsui T, Liu MC, Suiko M.
Biosci. Biotechnol. Biochem. 74 ( 11 ) 2253 - 2258 2010年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）