論文 - 榊原　陽一

Sulfation of environmental estrogen-like chemicals by human cytosolic sulfotransferases

Suiko, M., Sakakibara, Y., Liu, M.-C.

Biochemical and Biophysiccal ResearchCommunications Vol. 267, No. 180-84   2000年1月

Maturation activation and protection of dendritic cells induced by double-stranded RNA

Cella, M. , Salio, M., Sakakibara, Y. Langen, H.その他２名

Journal of Experimental MedicineVol. 189, No. 5821-829   1999年3月

Bacterial expression, purification, and characterization of a novel mouse sulfotransferase that catalyzes the sulfation of eicosanoids

Liu, M.-C., Sakakibara, Y., Liu, C.-C.

Biochemical and Biophysiccal ResearchCommunicationsVol. 254, No. 165-69   1999年1月

T lymphocyte costimulation mediated byreorganization of membrane microdomains

Viola, A., Schroeder, S., Sakakibara, Y. Lanzavecchia, A.

ScienceVol. 283, No. 5402680-682   1999年1月

Structural identification of sulfated tyrosine in human urine

Ranasinghe, J.G.S., Sakakibara, Y.Harada, M., Nishiyama, K.その他２名

Bioscience,Biotechnologyand BiochemistryVol. 63, No. 1229-231   1999年1月

Molecular cloning, expression, and characterization of novel human SULTIC sulfotransferases that catalyze the sulfonation of N-hydroxy-2- acetylaminofluorene 査読あり

Sakakibara Y., Yanagisawa K., Katafuchi J., Ringer D., Takami Y., Nakayama T., Suiko M., Liu M., Liu M.

Journal of Biological Chemistry   273 ( 51 )   33929 - 33935   1998年12月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Journal of Biological Chemistry  

Upon sulfonation, carcinogenic hydroxyarylamines such as N-hydroxy-2- acetylaminofiuorene (N-OH-2AAF) can be further activated to form ultimate carcinogens in vivo. Previous studies have shown that a SULT1C1 sulfotransferase is primarily responsible for the sulfonation of N-OH-2AAF in rat liver. In the present study, two novel human sulfotransferases shown to be members of the SULT1C sulfotransferase subfamily based on sequence analysis have been cloned, expressed, and characterized. Comparisons of the deduced amino acid sequence encoded by the human SULTIC sulfotransferase cDNA 1 reveal 63.7, 61.6, and 85.1% identity to the amino acid sequences of rat SULT1C1 sulfotransferase, mouse SULTIC1 sulfotransferase, and rabbit SULT1C sulfotransferase. In contrast, the deduced amino acid sequence of the human SULT1C sulfotransferase 2 cDNA displays 62.9, 63.1, 63.1, and 62.5% identity to the amino acid sequences of the human SULT1C sulfotransferase 1, rat SULT1C1 sulfotransferase, mouse SULT1C1 sulfotransferase, and rabbit SULT1C sulfotransferase. Recombinant human SULT1C sulfotransferases 1 and 2, expressed in Escherichia coli and purified to near electrophoretic homogeneity, were shown to cross-react with the antiserum against the rat liver SULT1C1 sulfotransferase and exhibited sulfonating activities with N- OH-2AAF as substrate. Tissue-specific expression of these novel human SULT1C sulfotransferases were examined by employing the Northern blotting technique. The results provide a foundation for the investigation into the functional relevance of these new SULT1C sulfotransferases in different human tissues/organs.

DOI： 10.1074/jbc.273.51.33929

Scopus

Molecular cloning, expression, and characterization of novel human SULT1C sulfotransferases that catalyze the sulfonation of N-hydroxy-2-acetylaminofluorene

Sakakibara, Y., Yanagisawa, K.Katafuchi, J., Ringer, D.P.その他４名

The Journal ofBiological ChemistryVol. 273, No. 5133929-33935   1998年12月

Substrate specificity of human monoamine (M)-form phenol sulfotransferase: Preparation and analysis of dopa 3-O-sulfate and dopa 4-O-sulfate

Suiko, M., Sakakibara, Y.Awan-Khan, R., Sakaida, H.その他３名

Journal ofBiochemistryVol. 124, No. 4707-711   1998年10月

Molecular cloning, expression, and characterization of a novel mouse liver SULT1B1 sulfotransferase

Saeki, Y., Sakakibara, Y., Araki, Y.Yanagisawa, K.その他３名

Journal ofBiochemistryVol. 124, No. 155-64   1998年7月

Molecular cloning, expression, and functional characterization of novel mouse sulfotransferases

Sakakibara, Y., Yanagisawa, K.Takami, Y., Nakayama, T.その他２名

Biochemical and Biophysiccal ResearchCommunicationsVol. 247, No. 3681-686   1998年6月

cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase enzyme

Yanagisawa, K., Sakakibara, Y.Suiko, M., Takami, Y.その他５名

Bioscience, Biotechnologyand BiochemistryVol. 62, No. 51037-1040   1998年5月

Localization and functional analysis of the substrate specificity/catalytic domains of human M-form and P-form phenol sulfotransferases

Sakakibara, Y., Takami, Y., Nakayama, T.Suiko, M., Liu, M.-C.

The Journal ofBiological ChemistryVol. 273, No. 116242-6247   1998年3月

Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites

Gowda, K., Black, S., Moeller, I.Sakakibara, Y.その他２名

Gene Vol. 207, No. 2197-207   1998年1月

cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/Adenosine 5′-phosphosulfate kinase enzyme 査読あり

Yanagisawa K., Sakakibara Y., Suiko M., Takami Y., Nakayama T., Nakajima H., Takayanagi K., Natori Y., Liu M.

Bioscience, Biotechnology and Biochemistry   62 ( 5 )   1037 - 1040   1998年

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Bioscience, Biotechnology and Biochemistry  

A cDNA encoding the human bifunctional ATP sulfurylase/adenosine 5′-phosphosulfate (APS) kinase was cloned and sequenced. The enzyme contains an APS kinase domain in its N-terminal portion and an ATP sulfurylase domain in its C-terminal portion. Recombinant full-length enzyme and its constituent APS kinase and ATP sulfurylase domains were individually expressed, purified, and shown to have their respective enzymatic activities. © 1998, Taylor & Francis Group, LLC. All rights reserved.

DOI： 10.1271/bbb.62.1037

Scopus

Characterization of bovine heart sulfotransferase catalyzing the sulfation of tyrosine-containing peptides

Suiko, M., Fern o, P.H.P.Sakakibara, Y., Kudo, H.その他２名

Journal of Nutritional Science andVitaminologyVol. 43, No. 3485-490   1997年8月

Tissue-specific and developmental stage-dependent expression of a novel rat dopa/tyrosine sulfotransferase

Araki, Y., Sakakibara, Y., Boggaram, B.Katafuchi, J.その他３名

International Journal of Biochemistry andCell BiologyVol. 29, No. 5801-806   1997年5月

Biochemistry of the sulfation of dopa and tyrosine isomers: investigation of a novel dopa/tyrosine sulfotransferase

Sakakibara, Y., Suiko, M., Nishiyama, K.Nakajima, H., Liu, M.-C.

Animal CellTechnology:Basic & Applied AspectsVol. 8309-314   1997年4月

Role of a putative tyrosine-O-sulfate receptor in the targeting and/or intracellular transport of tyrosine-sulfated proteins

Liu, M.-C., Sakakibara, Y., Suiko, M.

CytotechnologyVol. 23, No. 1-3143-149   1997年3月

Manganese-dependent Dopa/tyrosine sulfation in HepG2 human hepatoma cells: Novel Dopa/tyrosine sulfotransferase activities associated with the human monamine-form phenol sulfotransferase 査読あり

Sakakibara Y., Katafuchi J., Katafuchi J., Takami Y., Nakayama T., Suiko M., Suiko M., Nakajima H., Liu M.

Biochimica et Biophysica Acta - Molecular Cell Research   1355 ( 2 )   102 - 106   1997年2月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Biochimica et Biophysica Acta - Molecular Cell Research  

Human monoamine (M)-form phenol sulfotransferase (PST) was PCR-cloned and transiently expressed in COS-7 cells. The recombinant enzyme was demonstrated to display not only the previously reported sulfotransferase activity toward dopamine, but also novel manganese-dependent Dopa/tyrosine sulfotransferase activities. These results imply a new functional role of the human M-form PST in the homeostatic regulation of Dopa and tyrosine.

DOI： 10.1016/S0167-4889(96)00166-8

Scopus

Manganese-dependent dopa/tyrosine sulfation in HepG2 human hepatoma cells: novel dopa/tyrosine sulfotransferase activities associated with the human monoamine-form phenol sulfotransferase

Sakakibara, Y., Katafuchi, J.Takami, Y., Nakayama, T.その他２名

BiochimicaBiophysicaActaVol. 1355, No. 2102-106   1997年2月