論文 - 菅本 和寛
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Kai H, Okada Y, Goto Y, Nakayama T, Sugamoto K, Ogawa K, Yamasaki M, Morishita K, Matsuno K, Kunitake H
Plants (Basel, Switzerland) 11 ( 10 ) 2022年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plants
Although Vaccinium virgatum Aiton leaves and stems inhibit adult T-cell leukemia (ATL) cells, leaves and stems can differ between individual plants and by time and location. In this study, leaf and stem components were profiled in the same individual plant using direct-injection electron ionization-mass spectrometry (DI-EI-MS) metabolomics, with the aims of analyzing the anti-ATL activity, and quantifying proanthocyanidins (PACs). Leaves, stems, and leaf/stem mixtures showed distinct and characteristic spectra. Anti-ATL activity was stronger in stems than leaves, and the PAC content was higher in stems than leaves. These data were subjected to bivariate analysis to identify the factor (m/z) responsible for the inhibitory effect of ATL based on the highest coefficient of determination (R2 ). The results of this DI-EI-MS metabolomics analysis suggest that among PACs contained in V. virgatum stems and leaves, the fragment ion at m/z 149 contributes significantly to anti-ATL activity.
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Kai H., Sugamoto K., Toshima S., Goto Y., Nakayama T., Morishita K., Kunitake H.
Plants 11 ( 4 ) 2022年2月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plants
We previously reported that rabbit-eye blueberry (Vaccinium virgatum Aiton) leaves exhibit multiple functions. In this study, we evaluated whether V. virgatum stems can also be used as functional materials similar to leaves and clarified the major constituents and their biological activity (antioxidant activity and anti–adult T cell leukemia (ATL) activity). Water extracts of V. virgatum stems were separated into 19 fractions using a Diaion HP-20 open column. Sugars and organic acids were detected in the highly water-soluble fractions. Polyphenols and proanthocyanidin were detected in the hydrous methanol-soluble fractions. In biological activity evaluations, a difference in antioxidant activity was observed in the water-containing methanol-eluted fractions, and fractions exhibiting anti-ATL activity differed depending on cell type. These results suggest that blueberry stems, like leaves, are rich in polyphenols and exhibit antioxidant activity and inhibit ATL cell growth. In the future, aerial parts of blueberries, including stems and leaves, could be used as functional materials and/or medicinal resources.
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Ichikawa T., Sugamoto K., Matsuura Y., Kunitake H., Shimoda K., Morishita K.
Cancer Science 113 ( 4 ) 1406 - 1416 2022年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Cancer Science
We have previously reported that the proanthocyanidin (PAC) fraction of blueberry leaf extract (BB-PAC) inhibits the proliferation of HTLV-1-infected adult T-cell leukemia (ATL) by inducing apoptosis. In the present study, we further analyzed the structure of BB-PAC and elucidated the molecular mechanism underlying the inhibitory function of HTLV-1-infected and ATL cells. After hot water extraction with fractionation with methanol-acetone, BB-PAC was found to be concentrated in fractions 4 to 7 (Fr7). The strongest inhibition of ATL cell growth was observed with Fr7, which contained the highest BB-PAC polymerization degree of 14. The basic structure of BB-PAC is mainly B-type bonds, with A-type bonds (7.1%) and cinchonain I units as the terminal unit (6.1%). The molecular mechanism of cytotoxicity observed around Fr7 against ATL cells was the degradation of JAK1 to 3 and the dephosphorylation of STAT3/5, which occurs by proteasome-dependent proteolysis, confirming that PAC directly binds to heat shock protein 90 (HSP90). JAK degradation was caused by proteasome-dependent proteolysis, and we identified the direct binding of PAC to HSP90. In addition, the binding of cochaperone ATPase homolog 1 (AHA1) to HSP90, which is required for activation of the cofactor HSP90, was inhibited by BB-PAC treatment. Therefore, BB-PAC inhibited the formation of the HSP90/AHA1 complex and promoted the degradation of JAK protein due to HSP90 dysfunction. These results suggest that the highly polymerized PAC component from blueberry leaves has great potential as a preventive and therapeutic agent against HTLV-1-infected and ATL cells.
DOI： 10.1111/cas.15277
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Yamasaki M., Kiue Y., Fujii K., Sushida M., Yamasaki Y., Sugamoto K., Suzuki Y., Koga Y., Kunitake H., Kai H., Ogawa K., Nishiyama K., Goto Y., Nakayama T.
Plants 10 ( 12 ) 2021年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Plants
Blueberry (Vaccinium virgatum Aiton; Kinisato 35 Gou) leaves have recently attracted in-creasing attention as a useful material for the prevention of lifestyle diseases. Here, we examined the effects of the hot water extract of blueberry leaves (BLEx) on lipogenesis and uric acid production in 3T3-L1 adipocytes. The results showed that BLEx suppressed lipid accumulation and the mRNA expression of differentiation markers in 3T3-L1 adipocytes. A fractionation study showed that the highly polymerized proanthocyanidin-rich fraction was responsible for this effect. Upon maturation to adipocytes, 3T3-L1 cells produced uric acid and tumor necrosis factor-α, and hypoxia stimulated the production of uric acid and xanthine oxidoreductase activity. BLEx suppressed the production of uric acid under these conditions. Although BLEx inhibited the enzymatic activity of xanthine oxidase, this activity was observed in several fractions containing catechin, epicatechin, chlorogenic acid, rutin, and low molecular weight proanthocyanidins. Taken together, these results indicate that BLEx contains various compounds with the ability to suppress lipid accumulation and uric acid production in adipocytes.
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野菜ジュースカプセルを用いたカタラーゼ活性を視覚化した 体験教材の開発
菅本 和寛
宮崎大学工学部紀要 50 1 - 2 2021年9月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：宮崎大学工学部
The purpose of this study is to develop teaching materials for visualization of catalase activities. In this study, I
present a comparison of catalase activity in different encapsulated vegetable juices. Several vegetable juice
capsules were easily prepared from sodium alginate and calcium chloride. The catalase activity of vegetable juice capsules can be compared by measuring the floating time in H2O2 solution. -
野菜ジュースカプセルを用いたカタラーゼ活性を可視化した体験教材の開発 査読あり
菅本 和寛
宮崎大学工学部紀要 50 1 - 2 2021年9月
担当区分：筆頭著者, 責任著者 記述言語：日本語 掲載種別：研究論文（大学，研究機関等紀要）
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Matsumoto M., Ito T., Kanemaru S., Baba Y., Sugamoto K.
Solvent Extraction and Ion Exchange 39 ( 5-6 ) 573 - 583 2021年3月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Solvent Extraction and Ion Exchange
Demand for scandium (Sc) has been increasing in recent years. However, there are almost no ores containing Sc, and the supply is insufficient because the separation and recovery of Sc as a byproduct from oxide minerals such as ilmenite ore, bauxite, and laterite are difficult. In this study, extraction and separation of Sc from acidic chloride or acidic nitrate solutions containing transition metals such as Fe , Co , Ni , Zn , and Pd were studied using N-lauroylsarcosine (NLS) and tri-n-octylphosphine oxide (TOPO), which together create a “deep eutectic solvent” (DES). Owing to the respective selectivity of NLS and TOPO by different extractive interactions between metal ions and the two extractants in toluene, Sc was effectively and selectively separated even from Fe in the aqueous ammonium nitrate solution, the mutual separation of which is difficult with NLS alone. The mutual separation of Sc and Fe in acidic chloride solution is in general very difficult, while the mutual separation of these metals from acidic nitrate solution is successful. This is due to either anions forming complexes with Sc in the aqueous phase (chloride) or not (nitrate). The potential use of the combination of the NLS with TOPO for selective recovery of Sc from Fe in a refining process is demonstrated. 3+ 3+ 2+ 2+ 2+ 2+ 3+ 3+ 3+ 3+ 3+ 3+ 3+
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Dietary phytanic acid-induced changes in tissue fatty acid profiles in mice 査読あり
Nakanishi T., Kagamizono K., Yokoyama S., Suzuki R., Sakakibara H., Sugamoto K., Erickson L., Kawahara S.
Journal of Dairy Research 87 ( 4 ) 498 - 500 2020年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Dairy Research
The aims of this research communication were to investigate the in vivo tissue accumulation of phytanic acid (PA) and any changes in the tissue fatty acid profiles in mice. Previous in vitro studies have demonstrated that PA is a milk component with the potential to cause both beneficial effects on lipid and glucose metabolism and detrimental effects on neuronal cells. However, there is limited information about its in vivo actions. In this study, mice were fed diets containing either 0.00 or 0.05% 3RS, 7R, 11R-PA, which is the isomer found in milk and the human body. After 4 weeks, adipose tissue, liver and brain were harvested and their fatty acid profiles were determined by gas chromatographic analysis. The results showed that PA and its metabolite pristanic acid accumulated in the adipose tissue of PA-fed mice, and that dietary PA decreased the hepatic compositions of several saturated fatty acids such as palmitic acid while increasing the compositions of polyunsaturated fatty acids including linoleic acid and docosahexaenoic acid. However, dietary PA neither accumulated nor had a high impact on the fatty acid profile in the brain. These results suggested that dietary PA could exert its biological activities in adipose tissue and liver, although the brain is relatively less affected by dietary PA. These data provide a basis for understanding the in vivo physiological actions of PA.
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宮崎大学工学部で行った高校生「総合的な探究の時間」の評価と課題
田村 宏樹, 森田 千尋, 盆小原 康博, 荒井 昌和, 菅本 和寛, 坂本 眞人, 石津 貴弘
宮崎大学工学部紀要 49 17 - 22 2020年9月
記述言語：日本語 掲載種別：研究論文（大学，研究機関等紀要） 出版者・発行元：宮崎大学工学部
In 2019, we provided educational guidance for second-year students at Miyazaki Minami High School. We hope that this project will lead high school students to interest in the engineering field and lead to a change in awareness of research and development. In this paper, we introduce the six research projects for which research guidance was provided from profeesor of University of Miyzaki in 2019, and report on the evaluation of the research guidance and their issues.
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鹿屋 京平, 菅本 和寛, 馬場 由成, 塚崎 孝規
環境資源工学 67 ( 1 ) 5 - 12 2020年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：一般社団法人 環境資源工学会
The organic solvent is a liquid organic compound capable of dissolving a water-insoluble compound. In the solvent extraction method which is one of metal refining techniques, the large amount of organic solvent plays an important role not only to solubilize the extractant but also to affect the extraction behavior of metal complexes formed in the organic phase. However, most organic solvents have high volatility and toxicity, and their use is limited owing to health damage and environmental burden. In this study, we synthesized activated carbon impregnated extractant without organic solvent and aimed to separate Co and Ni. Specifically, activated carbon was impregnated with 2-isonitrosopropiophenone as extractant, and the effect on Ni and Co separation characteristics was investigated. As a result, the activated carbon was able to selectively adsorb Co over Ni compared to toluene diluent and that this activated carbon had high adsorption capacity for Co and leakage of extractant lower.
DOI： 10.4144/rpsj.67.5
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TKaede Yamasaki, Kazuhiro Sugamoto, Teruaki Arakawa, Kazuo Nishiyama1 and Masao Yamasaki
PeerJ 2019年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
Excessive alcohol consumption is a risk factor for liver diseases. Enhancement of alcohol
metabolism could be an effective strategy to prevent these adverse effects since it
promotes the clearance of ethanol and acetaldehyde from the serum. Polyphenol-rich
products have shown to protect against alcohol-related liver damage. Blueberry leaves
have attracted attention as they are rich polyphenols such as proantocyanidins and
chlorogenic acid. In this study, we investigated the effects of a high dose of blueberry
leaf extract (BLEx) on alcohol metabolism during chronic intake of ethanol. Sevenweek old Sprague-Dawley (SD) rats were divided into four groups: normal liquid diet
group (NLD), normal liquid diet + BLEx group (NLD + BLEx), alcohol liquid diet
group (ALD), and alcohol liquid diet + BLEx (ALD + BLEx). Then, rats were fed
experimental diet for 5 weeks and at the end of feeding period, body weight, food intake,
liver weight, indices of liver injury, expression and activity of alcohol metabolismrelated and anti-oxidative enzymes, and levels of carbonyl protein, triglyceride (TG),
and total cholesterol (T-Chol) were measured. Body weight and food intake decreased,
whereas liver aldehyde dehydrogenase (ALDH) activity, liver microsomal cytochrome
P450 2E1 (CYP2E1) protein and mRNA expression, and heme oxygenase 1 (HO-1)
mRNA expression were upregulated by ethanol intake. Dietary BLEx, however, did
not affect any of these ethanol-related changes. Indices of liver injury, expression and
activity of other alcohol metabolism-related enzymes, liver carbonyl protein, TG, and
T-Chol levels were not altered by ethanol and BLEx. Thus, chronic BLEx intake does
not ameliorate the harmful effects of ethanol.DOI： 10.7717/peerj.6989
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Terao R., Murata A., Sugamoto K., Watanabe T., Nagahama K., Nakahara K., Kondo T., Murakami N., Fukui K., Hattori H., Eto N.
Food and Function 10 ( 1 ) 38 - 48 2019年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Food and Function
© 2019 The Royal Society of Chemistry. Natural killer (NK) cells play an important role in the innate immune system by eliminating cancer cells and virally infected cells. Aging and stress attenuate the activity of NK cells, thereby increasing the risk of various diseases. In this study, we demonstrated that the consumption of a small number of kumquats in an in vivo model could suppress elevated plasma corticosterone levels and reverse the decline in splenocyte cytotoxicity caused by restraint stress. Our results identified β-cryptoxanthin (BCX) as an active kumquat component with a NK cell-activating effect, and R-limonene as an active component that mediates not only the anti-stress effect but also NK cell activation by oral administration. In addition, BCX, R-limonene, and R-limonene metabolites were found to enhance IFN-γ production in KHYG-1 cells, a human NK cell line. Collectively, our findings suggest that the ingestion of a few kumquats on a daily basis can help to combat stress and enhance NK cell activity.
DOI： 10.1039/c8fo01971a
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Nakanishi T., Motoba I., Anraku M., Suzuki R., Yamaguchi Y., Erickson L., Eto N., Sugamoto K., Matsushita Y., Kawahara S.
Lipids in Health and Disease 17 ( 1 ) 2018年6月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Lipids in Health and Disease
© 2018 The Author(s). Background: Among the eight stereoisomers of phytanic acid (PA), the 3RS, 7R, 11R-isomer is naturally occurring and is present in foods and the human body. PA is considered to have possible health benefits in the immune system. However, it remains undetermined whether these effects are elicited by the 3RS, 7R, 11R-PA isomer, because previous studies used a commercially available PA whose isomer configuration is unknown. In this study, we synthesized a preparation of 3RS, 7R, 11R-PA, and investigated its in vitro immunomodulatory effects, especially the T-cell production of interferon (IFN)-γ, which is associated with various autoimmune diseases. This study also investigated the effects of 3RS, 7R, 11R-PA on NF-κB activity in order to address the mechanism of its immunomodulatory effects. Methods: Mouse splenocytes and purified T-cells were stimulated with T-cell mitogens and incubated with 3RS, 7R, 11R-PA, followed by evaluation of IFN-γ production. The effect of 3RS, 7R, 11R-PA on NF-κB activity was also investigated using an A549 cell line with stable expression of an NF-κB-dependent luciferase reporter gene. Results: 3RS, 7R, 11R-PA significantly reduced in vitro IFN-γ production at both the protein and mRNA levels, and was accompanied by decreased expression of T-bet, a key regulator of Th1 cell differentiation. The results indicated that NF-κB-mediated transcriptional activity was significantly decreased by 3RS, 7R, 11R-PA and that GW6471, an antagonist of peroxisome proliferator activated receptor α (PPARα), abrogated the inhibitory effect of 3RS, 7R, 11R-PA on NF-κB activity. Conclusions: The present study suggests that 3RS, 7R, 11R-PA is a functional and bioactive fatty acid, and has a potentially beneficial effect for amelioration of T-cell mediated autoimmune diseases. This study also indicates that interference in the NF-κB pathway via PPARα activation is a potential mechanism of the immunomodulatory effects of 3RS, 7R, 11R-PA.
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Shiitake (Lentinula edodes) cultivation in sawdust media consisting of kunugi (Quercus acutissima) mixed with sugi (Cryptomeria japonica): optimization of gaseous phase rate in media by threephase- structure analysis 査読あり
Takeshi Nitta, Ichiro Kamei, Kazuhiro Sugamoto, Sadatoshi Meguro
Journal of Wood Science 62 ( 5 ) 452 - 459 2016年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
The effects of the gaseous phase rate of kunugi
(Quercus acutissima) sawdust media mixed with sugi
(Cryptomeria japonica) determined by a three-phasestructure
analysis of the fruiting body yields of shiitake
were investigated. The fruiting body yield on kunugi media
was significantly lower than that on commercially available
hardwood-sawdust-mixture (HSM) media with 64 % water
content. Three-phase-structure analysis showed that the
gaseous phase rate in kunugi media was lower than that in
HSM media. When the gaseous phase rate in kunugi media
was increased to the level in HSM media by decreasing the
water content to 56 %, the fruiting body yield on kunugi
media also increased. These results suggested that kunugi
sawdust could be used for shiitake cultivation if the gaseous
phase rate in the media was optimized. Because sugi
has a lower specific gravity and higher porosity than
kunugi, mixing sugi sawdust up to 30 % with kunugi media
caused an increase in the gaseous phase rate, and the
fruiting body yield reached the same level as that in HSM
media. These results suggested that kunugi media could be
used for shiitake cultivation by mixing with sugi sawdust. -
Nisigaki E., Sugamoto K., Nishida M., Matsushita Y.
Chemistry Letters 45 ( 7 ) 746 - 748 2016年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Chemistry Letters
© 2016 The Chemical Society of Japan. (+)-Pisiferic acid (1) was converted into (+)-12-O-methoxymethylpisiferol (5c) in 3 steps. The rearrangement of 5c with trifluoromethanesulfonic anhydride in pyridine provided (1)- 12-O-methoxymethylpisiferin (6c), which was converted into icetexane phenols 6a, 11, and 14. The ortho-selective oxygenation of 6a and 14 with stabilized 2-iodoxybenzoic acid (SIBX), followed by the reduction of intermediary quinones afforded (1)-barbatusol (2) and (+)-rosmaridiphenol (4) in 49% and 35%, respectively. The oxygenation of 11 using potassium 5-methyl- 2-iodobenzenesulfonate (pre-MIBS) with Oxone® followed by reduction gave (+)-demethylsalvicanol (3) in 59% yield.
DOI： 10.1246/cl.160221
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Suppressing effect of cordycepin on the lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells 「(共著)」 査読あり
Kenta Imamura, Momoko Asai, Kazuhiro Sugamoto, Tomoko Matsumoto, Yumi Yamasaki, Ichiro Kamei, Takahiro Hattori, Masaoki Kishimoto, Seiji Niisaka, Masaomi Kubo, Kazuo Nishiyama, Masao Yamasaki
Bioscience, Biotechnology, and Biochemistry 79 ( 6 ) 1021 - 1025 2015年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Imamura K., Asai M., Sugamoto K., Matsumoto T., Yamasaki Y., Kamei I., Hattori T., Kishimoto M., Niisaka S., Kubo M., Nishiyama K., Yamasaki M.
Bioscience, Biotechnology and Biochemistry 79 ( 6 ) 1021 - 1025 2015年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
© 2015 Japan Society for Bioscience, Biotechnology, and Agrochemistry. In this study, we focused on the anti-inflammatory effect of cordycepin, 3′-deoxyadenosine. Cordycepin potently suppressed nitric oxide production in lipo-polysaccharide-stimulated RAW 264.7 cells in an adenosine receptor-independent manner. In addition, inhibitors for adenosine kinase and nucleoside transporter abrogated the action of cordycepin. Thus, we considered that intracellular metabolism cordycepin is important for the anti-inflammatory effect of cordycepin.
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大学におけるアロマセラピーの取り組みとその現状-宮崎の未利用資源を活かした取り組み 招待あり
「菅本 和寛」 「松下 洋一」 「永田 祐子」 「廣澤 直也」
aromatopia 23 ( 2 ) 60 - 61 2014年3月
記述言語：日本語 掲載種別：研究論文（その他学術会議資料等）
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Nagahama K., Eto N., Sakakibara Y., Matsusita Y., Sugamoto K., Morishita K., Suiko M.
Journal of Functional Foods 6 ( 1 ) 356 - 366 2014年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Functional Foods
Adult T-cell leukemia/lymphoma (ATLL) is an aggressive disease caused by human T-cell lymphotropic virus type 1 (HTLV-1). As ATLL develops over many years, daily diet may play an important role in its occurrence. In this study, we investigated the growth inhibitory effect of extracts of blueberry leaf fractions on HTLV-1-associated cell lines. The most effective fraction contained highly oligomeric proanthocyanidins (OPA) with a mean degree of polymerization of 3.1. Cell growth inhibition by the OPA fraction was higher in all HTLV-1-associated cell lines than in the MOLT-4 HTLV-1-negative T-cells. It inhibited growth of the HTLV-1-infected T-cell line MT2 and ATLL-derived cell line Su9T01 by inducing apoptosis and G2/M cell cycle arrest. OPA fraction-induced apoptosis of HTLV-1-associated cells required death receptor-mediated caspase-dependent pathways. Treatment-induced G2/M arrest resulted from down-regulation of cyclin B1 and cdc2. The blueberry leaf OPA fraction may be a source of novel compounds for reducing the risk of developing ATLL. © 2013 Elsevier Ltd.
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Sasaki S., Tozawa T., Sugamoto K., Matsushita Y., Satoh T.
Bioscience, Biotechnology and Biochemistry 77 ( 10 ) 2131 - 2133 2013年11月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Bioscience, Biotechnology and Biochemistry
Cryptoquinone, an abietane-type diterpene paraquinone from the bark of Cryptomeria japonica has antifungal and cytotoxic activities, but its biological actions are largely unknown. In this study, we found that para-hydroquinone derivatives inhibited adipocyte differentiation. The actions might have been mediated, at least in part, by activation of the antioxidant-response element induction of phase 2 enzymes and increases in total glutathione.
DOI： 10.1271/bbb.130156