Papers - AKASHI Ryo
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Immunoadjuvant activity of crude lectin extracted from Momordica charantia Reviewed
15. Huang L, Ikejiri A, Shimizu Y, Adachi T, Goto Y, Toyama J, Tanaka H, Akashi R, Uchida K, Miyata H, Haga T
Journal of Veterinary Medical Science 70 533 - 535 2008.4
Language：English Publishing type：Research paper (scientific journal)
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Breeding of new Sweet pea cultivar 'Musica Rose' Reviewed
NAKAMURA K., HATTANDA N., FUKUMOTO K., AKASHI R.
7 ( 1 ) 2008.3
Language：English Publishing type：Research paper (scientific journal)
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Effect of peptized silica application on growth and mineral content in creeping bentgrass. Reviewed
Morishita F, Idota S, Akashi R
Bull. Fac. Agr. Univ. Miyazaki 5465 - 68 2008.3
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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Effects of peptized silica application on growth and mineral content in creeping bentgrass Reviewed
Morishita Fumiyasu, Idota Sachiko, Akashi Ryo
Bulletin of the Faculty of Agriculture, Miyazaki University 54 65 - 68 2008.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Miyazaki University
It is already reported that application of silica (SiO2) has some beneficial effects on plants such as rice, for example, to improve the photosynthetic activity, and pest and disease resistance, and to mitigate the environmental stresses, caused by the variations of climates, water and mineral contents of soil. In this study, the application of peptized silica solution ("SILICA-SUI" containing 4% of SiO2, Fuji Chemical Co., Ltd.) was examined for the growth and mineral contents, including of silica, in creeping bentgrass (Agrostis stolonifera L. cv. Penncross). In the leaf blade of aboveground parts, silica content increased with the increase in the concentration of silica solution. The concentration of silica in the leaf blade increase by 3 times as much as the non-applied control. The effect of concentration of silica solution was not apparently found on the nitrogen, phosphorous and potassium contents of leaf blade. In the underground roots, the active white root was detected in the putting green of creeping bentgrass by the application of silica solution. It is suggested that application of silica solution influence root of creeping bentgrass.
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Somatic embyogenesis and multiple-shoot formation from seed-derived shoot apical meristems of rhodesgrass (Chloris gayana Kunth). Reviewed
Gondo T, Matsumoto J, Yamakawa K, Tsuruta S, Ebina M, Akashi R
Grassland Science 53 138 - 142 2007.12
Language：English Publishing type：Research paper (scientific journal)
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Genetic relationship estimation in guineagrass (Panicum maximum Jacq) assessed on the basis of simple sequence repeat markers. Reviewed
Ebina M, Kouki K, Tsuruta S, Akashi R, Yamamoto T, Takahara M, Inafuku M, Okumura K, Nakagawa H, Nakajima K
Grassland Science 53 155 - 164 2007.12
Language：English Publishing type：Research paper (scientific journal)
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Ebina Masumi, Kouki Kaori, Tsuruta Shin-ichi, AKASHI Ryo, YAMAMOTO Toshiya, TAKAHARA Manabu, INAFUKU Masahito, OKUMURA Kenji, NAKAGAWA Hitoshi, NAKAJIMA Kousuke
Grassland science 53 ( 3 ) 155 - 164 2007.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Grassland Science
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Gondo Takahiro, Matsumoto Jun, Yamakawa Kazutomi, TSURUTA Shin-ichi, EBINA Masumi, AKASHI Ryo
Grassland science 53 ( 3 ) 138 - 142 2007.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Grassland Science
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Identification of plant species through the detection of the cell wall components using pyrolysis-gas chromatography. Reviewed
Uchiyama T, Kai S, Tanimura S, Sata K, Kawamura O, Akashi R
Japanese Journal of Science and Technology for Identification 12 185 - 196 2007.9
Language：Japanese Publishing type：Research paper (scientific journal)
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Quantitative trait locus analysis of multiple agronomic traits in the model legume Lotus japonicus. Reviewed
Gondo T, Sato S., Okumura K, Tabata S., Akashi R, Isobe S
Genome 50 627 - 637 2007.9
Language：English Publishing type：Research paper (scientific journal)
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Quantitative trait locus analysis of multiple agronomic traits in the model legume Lotus japonicus Reviewed
Gondo T., Sato S., Okumura K., Tabata S., Akashi R., Isobe S.
Genome 50 ( 7 ) 627 - 637 2007.7
Language：English Publishing type：Research paper (scientific journal) Publisher：Genome
The first quantitative trait locus (QTL) analysis of multiple agronomic traits in the model legume Lotus japonicus was performed with a population of recombinant inbred lines derived from Miyakojima MG-20 x Gifu B-129. Thirteen agronomic traits were evaluated in 2004 and 2005: traits of vegetative parts (plant height, stem thickness, leaf length, leaf width, plant regrowth, plant shape, and stem color), flowering traits (flowering time and degree), and pod and seed traits (pod length, pod width, seeds per pod, and seed mass). A total of 40 QTLs were detected that explained 5%-69% of total variation. The QTL that explained the most variation was that for stem color, which was detected in the same region of chromosome 2 in both years. Some QTLs were colocated, especially those for pod and seed traits. Seed mass QTLs were located at 5 locations that mapped to the corresponding genomic positions of equivalent QTLs in soybean, pea, chickpea, and mung bean. This study provides fundamental information for breeding of agronomically important legume crops. © 2007 NRC.
DOI： 10.1139/G07-040
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Analysis of genetic resource in Zoysia spp. 2. Indigenous to southwest islands of Japan based on simple sequence repeat markers. Reviewed
Hashiguchi M, Tsuruta S, Matsuo T, Ebina M, Kobayashi M, Akamine H, Akashi R
Jpn J Grassl Sci 53 133 - 137 2007.6
Language：Japanese Publishing type：Research paper (scientific journal)
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Isolation and characterization of cDNA encoding cinnamyl alcohol dehydrogenase (CAD) in sorghum (Sorghum bicolor (L.)Moench) Reviewed
Tsuruta S, Ebina M, Nakagawa H, Kawamura O, Akashi R
Grassland Science 53 103 - 106 2007.4
Language：English Publishing type：Research paper (scientific journal)
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Hashiguchi Masatsugu, Tsuruta Shin-ichi, Matsuo Taiyo, Ebina Masumi, Kobayashi Makoto, Akamine Hikaru, Akashi Ryo
Japanese Journal of Grassland Science 53 ( 2 ) 133 - 137 2007
Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Grassland Science
We conducted a study of genetic diversity in zoysiagrass indigenous to Southwest Islands of Japan based on simple sequence repeat (SSR) markers. Forty-one lines of zoysiagrass, which included 19 Zoysia matrella, 18 Z. tenuifolia and 4 cultivars (Asagake: Z.japonica, Emeralda: Z.japonica×Z.tenuifolia, Tottori-korai: Z matrella, Velvet: Z. tenuifolia), were used in this study. From 12 SSR markers used, a total of 155 SSR bands were scored. The number of putative alleles ranged from 6 to 22, with average value of 12.9.The polymorphic information contents (PIC) of Z.matrella and Z.tenuifolia were 0.79 and 0.72, respectively. Z.matrella had significantly higher PIC than Z.tenuifolia (p=0.02). Cluster analysis based on the 155 SSR bands revealed that the 41 lines were classified into 7 groups.
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Uchiyama Taketo, Kai Sayumi, Tanimura Shinichi, Sata Kazuhiro, Kawamura Osamu, Akashi Ryo
Japanese Journal of Forensic Science and Technology 12 ( 2 ) 185 - 196 2007
Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Association of Forensic Science and Technology
Identification of plant species is one of the clues, which is an important tool in criminal investigation. However, morphological test and DNA extraction are very difficult in minute plant pieces. Thus, it is necessary to develop a technique for classification and identification based on biological and chemical components, which does not require DNA analysis. This study analyzed the lignin of the cell wall components, which are products of secondary metabolism of a plant and also examined the species peculiarity and utility in forensic science. An analysis method of lignin is Pyrolysis-Gas chromatography (Py-GC). Analysis of trace amounts of dried plant samples using Py-GC shows results in waveform data, called pyrogram. Powdered samples each of 1.4 mg were dried, in all 22 plant species including <i>Gramineae</i> and <i>Leguminosae</i> were used for Py-GC. Thirteen characteristic peaks were chosen among a pyrogram of <i>Gramineaes</i> and it was converted into a bar graph and compared with other plant species. As a result, same plant species showed the similar pattern, and the same plant that was taken from a different place also showed the same pattern, and reproduction characteristics were recognized. Therefore, the possibility that difference in plant species is specific was shown by the pyrogram pattern. Even if it was the sample, in which DNA cannot be extracted, as for this method, a classification of a plant species was possible. Preprocessing of a sample is simple, as it has to only be dried. In conclusion, the application of this method in criminal investigation will involve swiftness and also forms a simple classification method for plant species.<br>
DOI： 10.3408/jafst.12.185
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Analysis of genetic resource in Zoysia spp. 1. Morphological characteristics and covering gain in zoysiagrass indigenous to southwest islands of Japan. Reviewed
Hashiguchi M, Tsuruta S, Matsuo T, Akamine H, Akashi R
Jpn J Grassl Sci 52 189 - 137 2006.12
Language：Japanese Publishing type：Research paper (scientific journal)
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Njoloma J., Tanaka K., Shimizu T., Nishiguchi T., Zakria M., Akashi R., Oota M., Akao S.
Biology and Fertility of Soils 43 ( 2 ) 137 - 143 2006.12
Language：English Publishing type：Research paper (scientific journal) Publisher：Biology and Fertility of Soils
In this study, we used Herbaspirillum sp. B501gfp1 (B501gfp1), an isolate from wild rice, to investigate the interaction between a non-host nitrogen-fixing endophytic bacterium and micropropagated sugarcane plants under aseptic condition. Two Japanese sugarcane plants (Saccharum sp.) cultivars (cvs) NiF8 and Ni15 were inoculated using B501gfp1 in two inoculum doses of 10 8 and 102 bacterial-cells-per-milliliter suspension. The results showed that bacterial cells colonized both the root and stem tissues, and colonization was apparent in the intercellular spaces. Higher bacterial numbers were detected in plant tissues inoculated with the higher inoculum concentration treatment. Bacterial numbers also varied between the two cultivars, with the higher values determined in cv Ni15. This study provides evidence that Herbaspirillum sp. B501gfp1, a rice isolate, could colonize sugarcane tissues, suggesting non-specificity of host plant among endophytes. © 2006 Springer-Verlag.
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Dry matter production and in vitro digestibility in BC1 progeny of bahiagrass (Paspalum notatum Flugge) regenerated from suspension culture. Reviewed
共著：K.Saleng, A. Sawai, T. Nagatani, Y. Tateyama, N. Mochidome, M. Niimi, R. Akashi, O. Kawamura
Grassland Science 52 (in press) 2006.8
Language：English Publishing type：Research paper (scientific journal)
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Saleng Kamaruddin, Sawai Akira, Nagatani Takeshi, TATEYAMA Yoshio, MOCHIDOME Nobuo, NIIMI Mitsuhiro, AKASHI Ryo, KAWAMURA Osamu
Grassland science 52 ( 2 ) 81 - 84 2006.6
Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Grassland Science
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DNA extraction from sperm found in mixed stains of seminal fluid and vaginal secretion using DNase I. Reviewed
Uciyama T, Hisazumi R, Maeda I, Nakata M, Ohno R, Hasegawa T, Shimizu K, Akashi R
日本法科学技術学会誌 11：105-112 11 105 - 112 2006.4
Language：Japanese Publishing type：Research paper (scientific journal)